qfilt

This simple program is meant to filter sequencing data with the option to:

• Remove or split reads with poor quality scores
• Retain only fragments from reads that are tagged with a given 5' sequence.

Example Output

>GM98SRO01B77KU rank=0000671 x=796.0 y=1996.0 length=58

CCACGCGTATCGATGTCGACTTTTTTTTCTTTTCTTACATAGTAG

>GM98SRO01BA3RP rank=0000953 x=419.5 y=1603.5 length=87

CTGATGCTGCACCAACTGTACTCCCTCGCGATA

>GM98SRO01E1BKW rank=0001233 x=1948.0 y=846.0 length=66

TACAGTTGGTGCAGCATCAGAAAAGTACGACATCGATACGCGTGGTCCTCGCGA

>GM98SRO01DVVNY rank=0001304 x=1476.0 y=636.5 length=84

ACGGCTGATGCTGCACCAACTGTACTCCCTCGCGATA

>GM98SRO01D6FIX rank=0001416 x=1596.0 y=1415.0 length=91

CGGCTGATGCTGCACCAACTGTACTCCCTCGCGATA