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Galaxy | Tool Preview

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Clip (version 1.0.3+galaxy2)

Input file in FASTA or FASTQ format:

Minimum sequence length (after clipping, sequences shorter than this length will be discarded:

Source:

Choose Adapter:

Enter a non-zero value to keep the adapter sequence and x bases that follow it:

Use this for hairpin barcoding. keep at 0 unless you know what you're doing

Discard sequences with unknown (N) bases:

Output options:

What it does

This tool clips adapters from the 3'-end of the sequences in a FASTA/FASTQ file.

Clipping Illustration:

Clipping Example:

In the above example:

Sequence no. 1 was discarded since it wasn't clipped (i.e. didn't contain the adapter sequence). (Output parameter).
Sequence no. 5 was discarded --- it's length (after clipping) was shorter than 15 nt (Minimum Sequence Length parameter).

This tool is based on FASTX-toolkit by Assaf Gordon.