Note. Database searches may take substantial amount of time. For large input datasets it is advisable to allow overnight processing.

What it does

This tool runs megablast function of BLAST+ blastn tool - a high performance nucleotide local aligner developed by Webb Miller and colleagues.

Output format

Output of this tool contains 13 columns delimited by Tabs:

1. Id of your sequence
2. GI of the database hit
3. Length of the database hit
4. % identity
5. Alignment length
6. # mismatches
7. # gaps
8. Start position in your sequence
9. End position in your sequence
10. Start position in database hit
11. End position in database hit
12. E-value
13. Bit score

Reference

Zhang et al. A Greedy Algorithm for Aligning DNA Sequences. 2000. JCB: 203-214.