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digest (version 5.0.0)
Sequence:
Enzyme/Reagent:
Trypsin will not normally cut after a K if it is followed by (e.g.) another K or a P. Specifying this shows those cuts, as well as the favoured ones:
Used for partial digestion. Shows all cuts from favoured cut sites plus 1..3, 2..4, 3..5 etc but not (e.g.) 2..5. Overlaps are therefore fragments with exactly one potential cut site within it:
As for overlap but fragments containing more than one potential cut site are included:
Output report file format:
You can view the original documentation
here
.