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Metastats (version 1.39.5.0)
design has 2 columns: group(col 1) and grouping(col 2) (separated by a TAB character) use make.design

Mothur Overview

Mothur is a comprehensive suite of tools for microbial ecology community. It is initiated by Dr. Patrick Schloss and his software development team in the Department of Microbiology and Immunology at The University of Michigan. For more information, see Mothur-Wiki.

Command Documentation

The metastats command generate principle components plot data.

v.1.21.0: Updated to mothur 1.33