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Split fastq libraries (version 1.9.1.0)
It must be one per input file path (used when data is not multiplexed)
Sample ID will be 'Unassigned'
This is applied after quality trimming, and is total over combined paired end reads if applicable
It is useful if barcodes in mapping file are reverse complements of golay codes
E.g., for Q20 and better, 19 must be specified

What it does

This tool performs demultiplexing of Fastq sequence data where barcodes and sequences are contained in two separate fastq files (common on Illumina runs).

More information about this tool is available on QIIME documentation.