Galaxy | Tool Preview

Convert (version 0.1.7.3)
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fastq input datasets
fastq input dataset 0
A SAM file with header information, as generated, for example, by the NGS Run Annotation Tool, that will be used to attach metainformation to the results file.

What it does

The tool converts between different file formats used for storing next-generation sequencing data.

As input file types it can handle uncompressed or gzipped fastq, SAM or BAM format, which it can convert to SAM or BAM format.

Notes:

  1. In its standard configuration Galaxy will decompress any .gz files during their upload, so the option to convert gzipped fastq input is useful only with customized Galaxy instances or by using linked files as explained in our recipe for using gzipped fastq files in Galaxy from the MiModD user guide.

  2. The tool can convert fastq files representing data from paired-end sequencing runs to appropriate SAM/BAM format provided that the mate information is split over two fastq files in corresponding order.

    TIP: If your paired-end data is arranged differently, you may look into the fastq splitter and fastq de-interlacer tools for Galaxy from the Fastq Manipulation category of the Galaxy Tool Shed to see if they can convert your files to the expected format.

  3. Merging partial fastq (or gzipped fastq) files into a single SAM/BAM file is supported both for single-end and paired-end data. Simply add additional input datasets and select the appropriate files (pairs of files in case of paired-end data).

    Concatenation of SAM/BAM file during conversion is currently not supported.

  4. For input in fastq format a SAM header file providing run metadata has to be specified. The information in this file will be used as the header data of the new SAM/BAM file. You can use the NGS Run Annotation tool to generate a new header file for your data.

    For input in SAM/BAM format the tool will simply copy the existing header data to the new file. To modify the header of an existing SAM/BAM file, use the Reheader BAM file tool instead.