Bracken Database Builder (version 2.8+galaxy0)

kraken_db:

Use Pre-built DB:

Use existing pre-built DB. This only works for kraken DBs, that already contain additional bracken DBs. This is the case for DBs downloaded from https://benlangmead.github.io/aws-indexes/k2.

K-mer length:

The K-mer length of the bracken DB should be identical to the K-mer length used to build the kraken2 DB.

Read length:

The read length of the bracken DB should be the same as the length of the reads to be analysis (one read in case of paired reads).

Database Name:

Please add a clear reference to the corresponding kraken2 DB the read length to the name.

A bracken DB can only be built as an addition to an existing kracken2 DB (see Bracken docs).

Using prebuilt DBs

The prebuilt option does use existing bracken DBs, that are shipped with kraken2 DBs. This is the case for DBs downloaded from https://benlangmead.github.io/aws-indexes/k2. All prebuilt databases contain a Kraken 2 database along with Bracken databases built for 50, 75, 100, 150, 200, 250 and 300 read lengths (refering to the lenght of the sequenced reads to be analysis using that database (one read in case of paired reads)). In this case the data manager points to the same DB as the kracken2 DB. The prebuilt option must not be used for custom kraken2 DBs ! For this the bracken DB needs to be build.

Building new DBs

Use the same K-mer length as the kraken2 DB and choose read lengths that are close to the read length of analysis you want to perform (one read in case of paired reads).