Purpose
The Trycycler subsample tool tries to make maximally-independent read subsets of an appropriate depth for your genome. The goal is to use each subsample for generating varios assemblies by using different assemblers. It will also be handy to know the approximate size of your genome. If you're assembling something novel (i.e. you don't know the genome size), you could do a quick initial assembly at this point to get an estimate.
Input
This tool requires a long-read dataset. Ideally your reads should have a coverage of at least 100x.
Output
The result is a number of subsampled read sets, whose number is determined by the count parameter.
Trycycler pipeline
Trycycler subsample: make maximally-independent read subsets of an appropriate depth. ⬇ Trycycler cluster: carries out complete-linkage clustering of all contig sequences. ⬇ Trycycler reconcile/msa: reconcile the contigs within each cluster and perform a multiple sequence alignment. ⬇ Trycycler partition: split the reads between the different clusters. ⬇ Trycycler consensus: generate a consensus contig sequence for each cluster.