qiime demux emp-paired (version 2019.4)

--m-barcodes-files

--m-barcodes-file 0

--i-seqs: ARTIFACT EMPPairedEndSequences The paired-end sequences to be demultiplexed. [required]:

--m-barcodes-column: COLUMN MetadataColumn[Categorical] The sample metadata column containing the per-sample barcodes. [required]:

--p-no-golay-error-correction: Do not perform 12nt Golay error correction on the barcode reads. [default: False]:

--p-rev-comp-barcodes: --p-no-rev-comp-barcodes If provided, the barcode sequence reads will be reverse complemented prior to demultiplexing. [default: False]:

--p-rev-comp-mapping-barcodes: --p-no-rev-comp-mapping-barcodes If provided, the barcode sequences in the sample metadata will be reverse complemented prior to demultiplexing. [default: False]:

Demultiplex paired-end sequence data generated with the EMP protocol.

Demultiplex paired-end sequence data (i.e., map barcode reads to sample ids) for data generated with the Earth Microbiome Project (EMP) amplicon sequencing protocol. Details about this protocol can be found at http://www.earthmicrobiome.org/protocols-and-standards/

Parameters

seqs : EMPPairedEndSequences: The paired-end sequences to be demultiplexed.
barcodes : MetadataColumn[Categorical]: The sample metadata column containing the per-sample barcodes.
golay_error_correction : Bool, optional: Perform 12nt Golay error correction on the barcode reads.
rev_comp_barcodes : Bool, optional: If provided, the barcode sequence reads will be reverse complemented prior to demultiplexing.
rev_comp_mapping_barcodes : Bool, optional: If provided, the barcode sequences in the sample metadata will be reverse complemented prior to demultiplexing.

Returns

per_sample_sequences : SampleData[PairedEndSequencesWithQuality]: The resulting demultiplexed sequences.
error_correction_details : ErrorCorrectionDetails: Detail about the barcode error corrections.