This tool works on binary images, so other image types will automatically be converted to binary
before they are analyzed. This step is performed using the ImageJ2 Make Binary command with
the following settings: Iterations: 1, Count: 1, Pad edges when eroding: No. The tool
allows you to choose the Black background setting. If these settings are not appropriate,
first manually convert the image to binary using the Convert to binary (black and white)
tool, which allows you to change them.
What it does
Counts and measures objects in binary or thresholded images. It works by scanning the image until
it finds the edge of an object. It then outlines the object, measures it, fills it to make it
invisible, then resumes scanning until it reaches the end of the image. Features of thresholded
images can be extracted by specifying suitable Size and Circularity ranges and/or by choosing
if particles should be traced by their outer edge or by flood filling.
- Size - Particles with size (area) outside the range specified in this field are ignored. Values may range between 0 and ‘Infinity’. Enter a single value and particles smaller than that value will be ignored.
- Cicularity - Particles with size circularity values outside the range specified in the min/max fields are also ignored.
- Show: Nothing - No image will be generated, but Display Results will be set to Yes. Note that the particle analyzer will display a blank image when the count of detected particles is zero and Show is not Nothing.
- Show: Outlines - Generates an 8-bit image containing numbered outlines of the measured particles (gray levels: Outlines: 0; Labels: 1; Background: 255).
- Show: Bare Outlines - Generates an 8-bit image containing simple outlines of the measured particles without labels (graylevels: Outlines: 0; Background: 255).
- Show: Ellipses - Generates an 8-bit image containing the best fit ellipse of each measured particle (gray levels: Ellipses: 0; Background: 255).
- Show: Masks - Generates an 8-bit image containing filled outlines of the measured particles (gray levels: Masks: 0; Background: 255).
- Show: Count Masks - Generates a 16-bit image containing filled outlines of the measured particles painted with a grayscale value corresponding to the particle number.
- Display Results - If Yes, the measurements for each particle will be generated.
- All results - If No, results will be restricted to the particle count, total particle area, average particle size, and area fraction.
- Exclude on Edges - If Yes, particles touching the edge of the image will be ignored.
- Include Holes - If Yes, interior holes will be included (set to No to exclude interior holes and to measure particles enclosed by other particles).