This tool works on binary images, so other image types will automatically be converted to binary before they are analyzed. This step is performed using the ImageJ2 Make Binary command with the following settings: Iterations: 1, Count: 1, Pad edges when eroding: No. The tool allows you to choose the Black background setting. If these settings are not appropriate, first manually convert the image to binary using the Convert to binary (black and white) tool, which allows you to change them.
What it does
Counts and measures objects in binary or thresholded images. It works by scanning the image until it finds the edge of an object. It then outlines the object, measures it, fills it to make it invisible, then resumes scanning until it reaches the end of the image. Features of thresholded images can be extracted by specifying suitable Size and Circularity ranges and/or by choosing if particles should be traced by their outer edge or by flood filling.