Pre-processing

APOSTL is able to recognize either a Scaffold "Samples Report" file (tab-delimited txt file) or the "peptides.txt" file output in the MaxQuant "txt" output folder. No modifications should be made to these files. Using the "Bait Create" tool, you can create your "bait.txt" file. It is important that the individual bait names match the bait names within your scaffold or MaxQuant output. APOSTL uses the bait file to findthe user's baits of interest. Additionally there is an option to make the prey file (Y/N). When making a prey file, APOSTL queries Uniprot ( http://www.uniprot.org ) in order to extract protein amino acidlengths and gene names. This takes several minutes depending on your internet connection. Some users may want to run SAINTexpress using the same data set while changing which baits are considered test or control It is useful to toggle "Make Prey" off in order to save time by circumventing this step as the same prey file can be used for both SAINTexpress runs.

INPUTS:

Scaffold file:

Scaffold "Samples Report" output (tab-delimited txt file)

MaxQuant file:

MaxQuant "peptides.txt" file (tab-delimited txt file)