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RNASeq samples quality check (version 2.15.1+galaxy0)
Output of abundance_estimates_to_matrix tool
File describing samples and replicates

Trinity assembles transcript sequences from Illumina RNA-Seq data. This tool performs some Quality Checks on a RNASeq experiment, analysing the abundance estimation for different samples using a transcriptome assembled with Trinity.

Inputs

This tool uses the matrix produced by 'Build expression matrix for a de novo assembly of RNA-Seq data by Trinity' tool.

You must describe your samples and replicates with a tabular file looking like this:

ConditionA CondA_replicate1
ConditionA CondA_replicate2
ConditionB CondB_replicate1
ConditionB CondB_replicate2
ConditionC CondC_replicate1
ConditionC CondC_replicate2
ConditionC CondC_replicate3

This file can be generated with the 'Describe samples and replicates' tool. The names in column 2 must match the names given in the tool 'Build expression matrix for a de novo assembly of RNA-Seq data by Trinity'.

Output

This tool will produce several PDF files, see the following page for more information: