Trinity assembles transcript sequences from Illumina RNA-Seq data. This tool performs some Quality Checks on a RNASeq experiment, analysing the abundance estimation for different samples using a transcriptome assembled with Trinity.
Inputs
This tool uses the matrix produced by 'Build expression matrix for a de novo assembly of RNA-Seq data by Trinity' tool.
You must describe your samples and replicates with a tabular file looking like this:
| ConditionA | CondA_replicate1 |
| ConditionA | CondA_replicate2 |
| ConditionB | CondB_replicate1 |
| ConditionB | CondB_replicate2 |
| ConditionC | CondC_replicate1 |
| ConditionC | CondC_replicate2 |
| ConditionC | CondC_replicate3 |
This file can be generated with the 'Describe samples and replicates' tool. The names in column 2 must match the names given in the tool 'Build expression matrix for a de novo assembly of RNA-Seq data by Trinity'.
Output
This tool will produce several PDF files, see the following page for more information: