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Sambamba flagstat (version 1.0.1+galaxy2)

Sambamba_flagstat outputs statistics drawn from read flags.

The first line contains numbers of QC-passed and QC-failed reads. Then come pairs of numbers, the former for QC-passed reads, the latter for QC-failed ones:
  • duplicates
  • mapped reads (plus percentage relative to the numbers from the first line)
  • reads with 'is_paired' flag set
  • paired reads which are first mates
  • paired reads which are second mates
  • paired reads with 'proper_pair' flag set (plus percentage relative to the numbers of QC-passed/failed reads with 'is_paired' flag set)
  • paired reads where both mates are mapped
  • paired reads where read itself is unmapped but mate is mapped
  • paired reads where mate is mapped to a different chromosome
  • the same as previous but mapping quality is not less than 5