Sambamba_flagstat outputs statistics drawn from read flags.
- The first line contains numbers of QC-passed and QC-failed reads. Then come pairs of numbers, the former for QC-passed reads, the latter for QC-failed ones:
- duplicates
- mapped reads (plus percentage relative to the numbers from the first line)
- reads with 'is_paired' flag set
- paired reads which are first mates
- paired reads which are second mates
- paired reads with 'proper_pair' flag set (plus percentage relative to the numbers of QC-passed/failed reads with 'is_paired' flag set)
- paired reads where both mates are mapped
- paired reads where read itself is unmapped but mate is mapped
- paired reads where mate is mapped to a different chromosome
- the same as previous but mapping quality is not less than 5