| Previous changeset 6:c7ff01bd331b (2024-06-21) |
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Commit message:
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/DIMet commit 6da96d865a3a557cfa3ad09e1cfa830519e73748 |
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modified:
dimet_metabologram.xml macros.xml |
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added:
test-data/AMINOACIDS-L-Cycloserine-T0-Control-T0--DEG_comparison1-abundances-cell.svg test-data/CENTRAL_CARBON_METABOLISM-L-Cycloserine-T0-Control-T0--DEG_comparison1-abundances-cell.svg |
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removed:
test-data/AMINOACIDS-Control-T0-L-Cycloserine-T0--DEG_comparison1-abundances-cell.svg test-data/CENTRAL_CARBON_METABOLISM-Control-T0-L-Cycloserine-T0--DEG_comparison1-abundances-cell.svg |
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| diff -r c7ff01bd331b -r 06f8d9ba09cd dimet_metabologram.xml --- a/dimet_metabologram.xml Fri Jun 21 18:49:45 2024 +0000 +++ b/dimet_metabologram.xml Tue Aug 06 17:36:05 2024 +0000 |
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| b'@@ -32,7 +32,7 @@\n colors_divergent_palette: [\'royalblue\', \'white\', \'red\'],\n edge_color: [\'black\',\'black\'],\n line_width:[\'1\',\'1.2\'],\n- display_label_and_value: true,\n+ display_label_and_value: ${output_options.write_values},\n font_size: ${output_options.font_size},\n fig_width: ${output_options.fig_width},\n figure_format:${output_options.figure_format},\n@@ -76,6 +76,7 @@\n <expand macro="input_parameters_metabologram"/>\n <expand macro="deg_list"/>\n <expand macro="compartments_metabologram"/>\n+\n <param name="correction_method" type="select" value="bonferroni" display="radio" label="Select multiple test correction to apply" help="Please enter at max 1 method">\n <option value="bonferroni">bonferroni</option>\n <option value="holm-sidak">holm-sidak</option>\n@@ -93,6 +94,8 @@\n <option value="pdf">Pdf</option>\n <option value="svg">Svg</option>\n </param>\n+ <param name="write_values" type="boolean" value="false" label="Write the values alongside the metabolites and genes names (e.g. L-Alanine: -0.44)"\n+ help="Default value is false."/>\n <param name="fig_width" type="integer" min="5" max="20" value="7" label="width of figures"\n help="Default value is 7."/>\n <param name="fig_height" type="integer" min="5" max="20" value="7" label="heigt of each figure"\n@@ -115,7 +118,6 @@\n <param name="path_kegg_transcripts" ftype="tabular" value="pathways_kegg_transcripts.csv"/>\n <param name="abundance_file" ftype="tabular" value="rawAbundances.csv"/>\n <param name="metadata_path" ftype="tabular" value="example2_metadata.csv"/>\n- <param name="metabolites_list" value="Fumaric_acid,Glycine,L-Proline"/>\n <param name="statistical_test_type" value="parametric"/>\n <param name="stat_test" value="Tt"/>\n <repeat name="deg_list">\n@@ -124,21 +126,22 @@\n <param name="valuecol" ftype="integer" value="3"/>\n <param name="timepoint" value=\'T0\'/>\n <repeat name="factor_list">\n- <param name="condition" value="Control"/>\n+ <param name="condition" value="L-Cycloserine"/>\n </repeat>\n <repeat name="factor_list">\n- <param name="condition" value="L-Cycloserine"/>\n+ <param name="condition" value="Control"/>\n </repeat>\n </repeat>\n <section name="output_options">\n+ <param name="show_values" value="false"/>\n <param name="figure_format" value="svg"/>\n <param name="figure_width" value="7"/>\n <param name="figure_height" value="7"/>\n <param name="font_size" value="12"/>\n </section>\n <output_collection name="report" type="list" count="3">\n- <element file="AMINOACIDS-Control-T0-L-Cycloserine-T0--DEG_comparison1-abundances-cell.svg" name="AMINOACIDS-Control-T0-L-Cycloserine-T0--DEG_comparison1-abundances-cell" ftype="svg" compare="sim_size" delta="100"/>\n- <element file="CENTRAL_CARBON_METABOLISM-Control-T0-L-Cycloserine-T0--DEG_comparison1-abundances-cell.svg" name="CENTRAL_CARBON_METABOLISM-Control-T0-L-Cycloserine-T0--DEG_comparison1-abundances-cell" ftype="svg" compare="sim_size" delta="100"/>\n+ <element file="AMINOACIDS-L-Cycloserine-T0-Control-T0--DEG_comparison1-abundances-cell.svg" name="AMINOACIDS-L-Cycloserine-T0-Control-T0--DEG_comparison1-abundances-cell" ftype="svg" compare="sim_size" delta="100"/>\n+ <element file="CENTRAL_CARBON_METABOLISM-L-Cycloserine-T0-Control-T0--DEG_comparison1-abundances-cell.svg" name="CENTRAL_CARBON_METABOLISM-L-Cycloserine-T0-Control-T0--DEG_compar'..b"provide the file with the results of the differential expression analysis of your transcriptomics data. It is recommended to use only the statistically significant **Differentially Expressed Genes (DEG)**. We provide examples in the zenodo (see section **Available data for testing**)\n+\n \n \n 3. **The pathways files**:\n@@ -296,16 +300,17 @@\n \n **Running the analysis**\n \n-You can precise how you want your analysis to be executed, with the parameters:\n+You can precise how you want your analysis to be executed with the parameters, which are of two types:\n \n+**a. Parameters proper to the metabolome differential analysis (that runs automatically before the integration)**:\n \n-a. Parameters proper to the metabolomics analysis (that runs automatically before the integration):\n+- **Conditions and timepoint**:\n \n-- **comparisons** : the pairs of [condition, timepoint] groups to compare\n+For each comparison to run on the metabolomics data, you can define the Conditions to compare, at a chosen time point, through the box 'Deregulated gene set': follow the instructions and choose the correct order of Conditions (the last must be the reference or control).\n+To add a second comparison, click the 'Insert Deregulated gene set' button.\n+You can insert and define as many boxes ('Deregulated gene set') as comparisons you want to perform.\n \n-- **datatypes** : the measures type that you want to run, that must be only one (see above in **Input data files** section)\n-\n-- **statistical_test** : choose the specific statistical test to be applied.\n+- **statistical_test**: choose the specific statistical test to be applied.\n \n Kruskal-Wallis, Mann-Whitney, Wilcoxon\xe2\x80\x99s signed rank test, Wilcoxon\xe2\x80\x99s rank sum test\n t-test, and permutation test are currently offered (we use the trusted functions from scipy library https://docs.scipy.org/doc/scipy/reference/stats.html).\n@@ -318,19 +323,24 @@\n whereas if 'distance/span' < 0 there is no separation.\n To use with caution in case of important dispersion of your intra-group values. Default is -0.3 (not stringent)\n \n-- **correction_method** : one of the methods for multiple testing correction available in statsmodels library (bonferroni, fdr_bh, sidak, among others, see https://www.statsmodels.org/dev/generated/statsmodels.stats.multitest.multipletests.html).\n+- **correction_method**: one of the methods for multiple testing correction available in statsmodels library (bonferroni, fdr_bh, sidak, among others, see https://www.statsmodels.org/dev/generated/statsmodels.stats.multitest.multipletests.html).\n \n-- **compartment** : one of the compartments present in your data.\n+- **compartment**: one of the compartments present in your data.\n \n-b. Parameters proper to the integration (that runs automatically after the metabolites analysis):\n+**b. Parameters proper to the integration with transcriptomics (that runs automatically after the metabolome differential analysis)**:\n \n+- **Deregulated genes set file**: Corresponds to the transcriptomics data, as explained in **Input data files** section.\n \n-- **transcripts** : the file(s) with the results of the differential expression analysis. They must be as many as the number of comparisons (metabolomics analysis) and keep a coherent order with them.\n+For each 'Deregulated gene set' box, a single **Differentially Expressed Genes (DEG)** file must be added, and must match with the metabolomics comparison set in the same box. Additional files can be added with the 'Insert deregulated gene set' button,\n+see also the subsection 'Conditions and timepoint' above.\n \n - **pathways** : files for the pathways, as explained in **Input data files** section\n \n \n-There exist hints on use that will guide you, next to the parameters.\n+Overall, the metabologram module provides hints on use that will guide you, in the same menus and boxes of the parameters.\n+\n+\n+**Output**\n \n The output consists of one figure by metabologram, and a color key bar legend valid for all metabolograms produced\n \n" |
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| diff -r c7ff01bd331b -r 06f8d9ba09cd macros.xml --- a/macros.xml Fri Jun 21 18:49:45 2024 +0000 +++ b/macros.xml Tue Aug 06 17:36:05 2024 +0000 |
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| @@ -1,6 +1,6 @@ <macros> <token name="@TOOL_VERSION@">0.2.4</token> - <token name="@VERSION_SUFFIX@">3</token> + <token name="@VERSION_SUFFIX@">4</token> <xml name="requirements"> <requirements> <requirement type="package" version="@TOOL_VERSION@">dimet</requirement> @@ -49,6 +49,7 @@ </xml> <xml name="citations"> <citations> + <citation type="doi">10.1093/bioinformatics/btae282</citation> <citation type="bibtex"> @software{Galvis_Rodriguez_DIMet, author = {Galvis Rodriguez, Johanna and Guyon, Joris and Dartigues, Benjamin and Specque, Florian and Daubon, Thomas and Karkar, Slim and Nikolski, Macha}, @@ -56,7 +57,6 @@ title = {{DIMet}}, url = {https://github.com/cbib/DIMet} } - </citation> </citations> @@ -95,58 +95,23 @@ </xml> <xml name="input_parameters_metabologram"> <conditional name="data_input"> - <param name="data_input_selector" type="select" label="Abundance, Enrichment or Isotopologues quantification files" help="Select between raw abundance and mean enrichment files"> + <param name="data_input_selector" type="select" label="Abundance or Enrichment quantification files" help="Select between metabolite total abundances and mean enrichment files"> <option value="abundance" selected="True">abundance</option> <option value="mean_enrichment">mean_enrichment</option> </param> <when value="abundance"> <expand macro="abundance_file_macros"/> - <param name="metabolites_list" type="select" optional="false" multiple="true" - label="Select Metabolite(s) for condition 1 to plot (1 Min.). You have to load a abundance file prior to have access to metabolite list"> - <validator type="length" min="1" message="Please enter at max 2 compartments"/> - <options from_dataset="abundance_file"> - <column name="metabolite_or_isotopologue" index="0"/> - <column name="value" index="0"/> - <filter type="unique_value" name="metabolite_or_isotopologue" column="0"/> - <filter type="remove_value" value="metabolite_or_isotopologue"/> - </options> - <sanitizer> - <valid initial="default"> - <add preset="string.printable"/> - <add value="\t"/> - <remove value="""/> - <remove value="'"/> - </valid> - </sanitizer> - </param> <expand macro="statistical_test"/> + </when> <when value="mean_enrichment"> <expand macro="enrichment_file_macros"/> - <param name="metabolites_list" type="select" optional="false" multiple="true" - label="Select Metabolite(s) for condition 1 to plot (1 Min.). You have to load a abundance file prior to have access to metabolite list"> - <validator type="length" min="1" message="Please enter at max 2 compartments"/> - <options from_dataset="me_or_frac_contrib_file"> - <column name="metabolite_or_isotopologue" index="0"/> - <column name="value" index="0"/> - <filter type="unique_value" name="metabolite_or_isotopologue" column="0"/> - <filter type="remove_value" value="metabolite_or_isotopologue"/> - </options> - <sanitizer> - <valid initial="default"> - <add preset="string.printable"/> - <add value="\t"/> - <remove value="""/> - <remove value="'"/> - </valid> - </sanitizer> - </param> <expand macro="statistical_test"/> </when> </conditional> + <expand macro="metadata_file_macros"/> <param name="path_kegg_metabolites" type="data" format="tabular" label="Pathways kegg metabolites file" help="A file with the pathways and respective metabolites ID, that must match with those in your metabolomics data. The names of the columns must be the pathways' names, see the minimal data example downloaded from zenodo as explained above. (see help below for more details)"/> <param name="path_kegg_transcripts" type="data" format="tabular" label="Pathways kegg transcripts file" help="A file with the pathways and respective gene symbols, which must match with those present in the transcriptomics data. The names of the columns must be the pathways' names, see the minimal data example downloaded from zenodo as explained above. (see help below for more details)"/> - <expand macro="metadata_file_macros"/> </xml> <xml name="input_parameters_bivar_analysis"> <conditional name="data_input"> |
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| diff -r c7ff01bd331b -r 06f8d9ba09cd test-data/AMINOACIDS-Control-T0-L-Cycloserine-T0--DEG_comparison1-abundances-cell.svg --- a/test-data/AMINOACIDS-Control-T0-L-Cycloserine-T0--DEG_comparison1-abundances-cell.svg Fri Jun 21 18:49:45 2024 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 |
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| diff -r c7ff01bd331b -r 06f8d9ba09cd test-data/AMINOACIDS-L-Cycloserine-T0-Control-T0--DEG_comparison1-abundances-cell.svg --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/AMINOACIDS-L-Cycloserine-T0-Control-T0--DEG_comparison1-abundances-cell.svg Tue Aug 06 17:36:05 2024 +0000 |
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