| Next changeset 1:ec202446408a (2014-06-04) |
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Commit message:
Uploaded v0.0.3, first stable release. |
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test-data/demo_nuc_align.fasta test-data/demo_nucs.fasta test-data/demo_nucs_trailing_stop.fasta test-data/demo_prot_align.fasta tools/align_back_trans/README.rst tools/align_back_trans/align_back_trans.py tools/align_back_trans/align_back_trans.xml tools/align_back_trans/tool_dependencies.xml |
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| diff -r 000000000000 -r 0c24e4e2177d test-data/demo_nuc_align.fasta --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/demo_nuc_align.fasta Thu Mar 06 08:58:13 2014 -0500 |
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| @@ -0,0 +1,6 @@ +>Alpha +GATGAGGAACGA +>Beta +GATGAG---CGU +>Gamma +GAT------CGG |
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| diff -r 000000000000 -r 0c24e4e2177d test-data/demo_nucs.fasta --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/demo_nucs.fasta Thu Mar 06 08:58:13 2014 -0500 |
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| @@ -0,0 +1,6 @@ +>Alpha +GATGAGGAACGA +>Beta +GATGAGCGU +>Gamma +GATCGG |
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| diff -r 000000000000 -r 0c24e4e2177d test-data/demo_nucs_trailing_stop.fasta --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/demo_nucs_trailing_stop.fasta Thu Mar 06 08:58:13 2014 -0500 |
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| @@ -0,0 +1,6 @@ +>Alpha +GATGAGGAACGA +>Beta +GATGAGCGU +>Gamma +GATCGGTAA |
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| diff -r 000000000000 -r 0c24e4e2177d test-data/demo_prot_align.fasta --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/demo_prot_align.fasta Thu Mar 06 08:58:13 2014 -0500 |
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| @@ -0,0 +1,6 @@ +>Alpha +DEER +>Beta +DE-R +>Gamma +D--R |
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| diff -r 000000000000 -r 0c24e4e2177d tools/align_back_trans/README.rst --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tools/align_back_trans/README.rst Thu Mar 06 08:58:13 2014 -0500 |
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| @@ -0,0 +1,108 @@ +Galaxy tool to back-translate a protein alignment to nucleotides +================================================================ + +This tool is copyright 2012-2014 by Peter Cock, The James Hutton Institute +(formerly SCRI, Scottish Crop Research Institute), UK. All rights reserved. +See the licence text below (MIT licence). + +This tool is a short Python script (using Biopython library functions) to +load a protein alignment, and matching nucleotide FASTA file of unaligned +sequences, which are threaded onto the protein alignment in order to produce +a codon aware nucleotide alignment - which can be viewed as a back translation. + +This tool is available from the Galaxy Tool Shed at: + +* http://toolshed.g2.bx.psu.edu/view/peterjc/align_back_trans + + +Automated Installation +====================== + +This should be straightforward using the Galaxy Tool Shed, which should be +able to automatically install the dependency on Biopython, and then install +this tool and run its unit tests. + + +Manual Installation +=================== + +There are just two files to install to use this tool from within Galaxy: + +* ``align_back_trans.py`` (the Python script) +* ``align_back_trans.xml`` (the Galaxy tool definition) + +The suggested location is in a dedicated ``tools/align_back_trans`` folder. + +You will also need to modify the ``tools_conf.xml`` file to tell Galaxy to offer +the tool. One suggested location is in the multiple alignments section. Simply +add the line:: + + <tool file="align_back_trans/align_back_trans.xml" /> + +You will also need to install Biopython 1.62 or later. If you want to run +the unit tests, include this line in ``tools_conf.xml.sample`` and the sample +FASTA files under the ``test-data`` directory. Then:: + + ./run_functional_tests.sh -id align_back_trans + +That's it. + + +History +======= + +======= ====================================================================== +Version Changes +------- ---------------------------------------------------------------------- +v0.0.1 - Initial version, based on a previously written Python script +v0.0.2 - Optionally check the translation is consistent +======= ====================================================================== + + +Developers +========== + +This script was initially developed on this repository: +https://github.com/peterjc/picobio/blob/master/align/align_back_trans.py + +With the addition of a Galaxy wrapper, developement moved here: +https://github.com/peterjc/pico_galaxy/tree/master/tools/align_back_trans + +For making the "Galaxy Tool Shed" http://toolshed.g2.bx.psu.edu/ tarball use +the following command from the Galaxy root folder:: + + $ tar -czf align_back_trans.tar.gz tools/align_back_trans/README.rst tools/align_back_trans/align_back_trans.py tools/align_back_trans/align_back_trans.xml tools/align_back_trans/tool_dependencies.xml test-data/demo_nucs.fasta test-data/demo_nucs_trailing_stop.fasta test-data/demo_prot_align.fasta test-data/demo_nuc_align.fasta + +Check this worked:: + + $ tar -tzf align_back_trans.tar.gz + tools/align_back_trans/README.rst + tools/align_back_trans/align_back_trans.py + tools/align_back_trans/align_back_trans.xml + tools/align_back_trans/tool_dependencies.xml + test-data/demo_nucs.fasta + test-data/demo_nucs_trailing_stop.fasta + test-data/demo_prot_align.fasta + test-data/demo_nuc_align.fasta + + +Licence (MIT) +============= + +Permission is hereby granted, free of charge, to any person obtaining a copy +of this software and associated documentation files (the "Software"), to deal +in the Software without restriction, including without limitation the rights +to use, copy, modify, merge, publish, distribute, sublicense, and/or sell +copies of the Software, and to permit persons to whom the Software is +furnished to do so, subject to the following conditions: + +The above copyright notice and this permission notice shall be included in +all copies or substantial portions of the Software. + +THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR +IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, +FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE +AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER +LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, +OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN +THE SOFTWARE. |
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| diff -r 000000000000 -r 0c24e4e2177d tools/align_back_trans/align_back_trans.py --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tools/align_back_trans/align_back_trans.py Thu Mar 06 08:58:13 2014 -0500 |
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| @@ -0,0 +1,193 @@ +#!/usr/bin/env python +"""Back-translate a protein alignment to nucleotides + +This tool is a short Python script (using Biopython library functions) to +load a protein alignment, and matching nucleotide FASTA file of unaligned +sequences, in order to produce a codon aware nucleotide alignment - which +can be viewed as a back translation. + +The development repository for this tool is here: + +* https://github.com/peterjc/pico_galaxy/tree/master/tools/align_back_trans + +This tool is available with a Galaxy wrapper from the Galaxy Tool Shed at: + +* http://toolshed.g2.bx.psu.edu/view/peterjc/align_back_trans + +See accompanying text file for licence details (MIT licence). + +This is version 0.0.3 of the script. +""" + +import sys +from Bio.Seq import Seq +from Bio.Alphabet import generic_dna, generic_protein +from Bio.Align import MultipleSeqAlignment +from Bio import SeqIO +from Bio import AlignIO +from Bio.Data.CodonTable import ambiguous_generic_by_id + +if "-v" in sys.argv or "--version" in sys.argv: + print "v0.0.3" + sys.exit(0) + +def stop_err(msg, error_level=1): + """Print error message to stdout and quit with given error level.""" + sys.stderr.write("%s\n" % msg) + sys.exit(error_level) + +def check_trans(identifier, nuc, prot, table): + """Returns nucleotide sequence if works (can remove trailing stop)""" + if len(nuc) % 3: + stop_err("Nucleotide sequence for %s is length %i (not a multiple of three)" + % (identifier, nuc)) + + p = str(prot).upper().replace("*", "X") + t = str(nuc.translate(table)).upper().replace("*", "X") + if len(t) == len(p) + 1: + if str(nuc)[-3:].upper() in ambiguous_generic_by_id[table].stop_codons: + #Allow this... + t = t[:-1] + nuc = nuc[:-3] #edit return value + if len(t) != len(p) and p in t: + stop_err("%s translation matched but only as subset of nucleotides, " + "wrong start codon?" % identifier) + if len(t) != len(p) and p[1:] in t: + stop_err("%s translation matched (ignoring first base) but only " + "as subset of nucleotides, wrong start codon?" % identifier) + if len(t) != len(p): + stop_err("Inconsistent lengths for %s, ungapped protein %i, " + "tripled %i vs ungapped nucleotide %i" % + (identifier, + len(p), + len(p) * 3, + len(nuc))) + + + if t == p: + return nuc + elif p.startswith("M") and "M" + t[1:] == p: + #Close, was there a start codon? + if str(nuc[0:3]).upper() in ambiguous_generic_by_id[table].start_codons: + return nuc + else: + stop_err("Translation check failed for %s\n" + "Would match if %s was a start codon (check correct table used)\n" + % (identifier, nuc[0:3].upper())) + else: + #Allow * vs X here? e.g. internal stop codons + m = "".join("." if x==y else "!" for (x,y) in zip(p,t)) + if len(prot) < 70: + sys.stderr.write("Protein: %s\n" % p) + sys.stderr.write(" %s\n" % m) + sys.stderr.write("Translation: %s\n" % t) + else: + for offset in range(0, len(p), 60): + sys.stderr.write("Protein: %s\n" % p[offset:offset+60]) + sys.stderr.write(" %s\n" % m[offset:offset+60]) + sys.stderr.write("Translation: %s\n\n" % t[offset:offset+60]) + stop_err("Translation check failed for %s\n" % identifier) + +def sequence_back_translate(aligned_protein_record, unaligned_nucleotide_record, gap, table=0): + #TODO - Separate arguments for protein gap and nucleotide gap? + if not gap or len(gap) != 1: + raise ValueError("Please supply a single gap character") + + alpha = unaligned_nucleotide_record.seq.alphabet + if hasattr(alpha, "gap_char"): + gap_codon = alpha.gap_char * 3 + assert len(gap_codon) == 3 + else: + from Bio.Alphabet import Gapped + alpha = Gapped(alpha, gap) + gap_codon = gap*3 + + ungapped_protein = aligned_protein_record.seq.ungap(gap) + ungapped_nucleotide = unaligned_nucleotide_record.seq + if table: + ungapped_nucleotide = check_trans(aligned_protein_record.id, ungapped_nucleotide, ungapped_protein, table) + elif len(ungapped_protein) * 3 != len(ungapped_nucleotide): + stop_err("Inconsistent lengths for %s, ungapped protein %i, " + "tripled %i vs ungapped nucleotide %i" % + (aligned_protein_record.id, + len(ungapped_protein), + len(ungapped_protein) * 3, + len(ungapped_nucleotide))) + + seq = [] + nuc = str(ungapped_nucleotide) + for amino_acid in aligned_protein_record.seq: + if amino_acid == gap: + seq.append(gap_codon) + else: + seq.append(nuc[:3]) + nuc = nuc[3:] + assert not nuc, "Nucleotide sequence for %r longer than protein %r" \ + % (unaligned_nucleotide_record.id, aligned_protein_record.id) + + aligned_nuc = unaligned_nucleotide_record[:] #copy for most annotation + aligned_nuc.letter_annotation = {} #clear this + aligned_nuc.seq = Seq("".join(seq), alpha) #replace this + assert len(aligned_protein_record.seq) * 3 == len(aligned_nuc) + return aligned_nuc + +def alignment_back_translate(protein_alignment, nucleotide_records, key_function=None, gap=None, table=0): + """Thread nucleotide sequences onto a protein alignment.""" + #TODO - Separate arguments for protein and nucleotide gap characters? + if key_function is None: + key_function = lambda x: x + if gap is None: + gap = "-" + + aligned = [] + for protein in protein_alignment: + try: + nucleotide = nucleotide_records[key_function(protein.id)] + except KeyError: + raise ValueError("Could not find nucleotide sequence for protein %r" \ + % protein.id) + aligned.append(sequence_back_translate(protein, nucleotide, gap, table)) + return MultipleSeqAlignment(aligned) + + +if len(sys.argv) == 4: + align_format, prot_align_file, nuc_fasta_file = sys.argv[1:] + nuc_align_file = sys.stdout + table = 0 +elif len(sys.argv) == 5: + align_format, prot_align_file, nuc_fasta_file, nuc_align_file = sys.argv[1:] + table = 0 +elif len(sys.argv) == 6: + align_format, prot_align_file, nuc_fasta_file, nuc_align_file, table = sys.argv[1:] +else: + stop_err("""This is a Python script for 'back-translating' a protein alignment, + +It requires three, four or five arguments: +- alignment format (e.g. fasta, clustal), +- aligned protein file (in specified format), +- unaligned nucleotide file (in fasta format). +- aligned nucleotiode output file (in same format), optional. +- NCBI translation table (0 for none), optional + +The nucleotide alignment is printed to stdout if no output filename is given. + +Example usage: + +$ python align_back_trans.py fasta demo_prot_align.fasta demo_nucs.fasta demo_nuc_align.fasta + +Warning: If the output file already exists, it will be overwritten. + +This script is available with sample data and a Galaxy wrapper here: +https://github.com/peterjc/pico_galaxy/tree/master/tools/align_back_trans +http://toolshed.g2.bx.psu.edu/view/peterjc/align_back_trans +""") + +try: + table = int(table) +except: + stop_err("Bad table argument %r" % table) + +prot_align = AlignIO.read(prot_align_file, align_format, alphabet=generic_protein) +nuc_dict = SeqIO.index(nuc_fasta_file, "fasta") +nuc_align = alignment_back_translate(prot_align, nuc_dict, gap="-", table=table) +AlignIO.write(nuc_align, nuc_align_file, align_format) |
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| diff -r 000000000000 -r 0c24e4e2177d tools/align_back_trans/align_back_trans.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tools/align_back_trans/align_back_trans.xml Thu Mar 06 08:58:13 2014 -0500 |
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| @@ -0,0 +1,131 @@ +<tool id="align_back_trans" name="Thread nucleotides onto a protein alignment (back-translation)" version="0.0.3"> + <description>Gives a codon aware alignment</description> + <requirements> + <requirement type="package" version="1.63">biopython</requirement> + <requirement type="python-module">Bio</requirement> + </requirements> + <version_command interpreter="python">align_back_trans.py --version</version_command> + <command interpreter="python"> +align_back_trans.py $prot_align.ext "$prot_align" "$nuc_file" "$out_nuc_align" "$table" + </command> + <stdio> + <!-- Anything other than zero is an error --> + <exit_code range="1:" /> + <exit_code range=":-1" /> + </stdio> + <inputs> + <param name="prot_align" type="data" format="fasta,muscle,clustal" label="Aligned protein file" help="Mutliple sequence file in FASTA, ClustalW or PHYLIP format." /> + <param name="table" type="select" label="Genetic code" help="Tables from the NCBI, these determine the start and stop codons"> + <option value="1">1. Standard</option> + <option value="2">2. Vertebrate Mitochondrial</option> + <option value="3">3. Yeast Mitochondrial</option> + <option value="4">4. Mold, Protozoan, Coelenterate Mitochondrial and Mycoplasma/Spiroplasma</option> + <option value="5">5. Invertebrate Mitochondrial</option> + <option value="6">6. Ciliate Macronuclear and Dasycladacean</option> + <option value="9">9. Echinoderm Mitochondrial</option> + <option value="10">10. Euplotid Nuclear</option> + <option value="11">11. Bacterial</option> + <option value="12">12. Alternative Yeast Nuclear</option> + <option value="13">13. Ascidian Mitochondrial</option> + <option value="14">14. Flatworm Mitochondrial</option> + <option value="15">15. Blepharisma Macronuclear</option> + <option value="16">16. Chlorophycean Mitochondrial</option> + <option value="21">21. Trematode Mitochondrial</option> + <option value="22">22. Scenedesmus obliquus</option> + <option value="23">23. Thraustochytrium Mitochondrial</option> + <option value="0">Don't check the translation</option> + </param> + <param name="nuc_file" type="data" format="fasta" label="Unaligned nucleotide sequences" help="FASTA format, using same identifiers as your protein alignment" /> + </inputs> + <outputs> + <data name="out_nuc_align" format="fasta" label="${prot_align.name} (back-translated)"> + <!-- TODO - Replace this with format="input:prot_align" if/when that works --> + <change_format> + <when input_dataset="prot_align" attribute="extension" value="clustal" format="clustal" /> + <when input_dataset="prot_align" attribute="extension" value="phylip" format="phylip" /> + </change_format> + </data> + </outputs> + <tests> + <test> + <param name="prot_align" value="demo_prot_align.fasta" /> + <param name="nuc_file" value="demo_nucs.fasta" /> + <param name="table" value="0" /> + <output name="out_nuc_align" file="demo_nuc_align.fasta" /> + </test> + <test> + <param name="prot_align" value="demo_prot_align.fasta" /> + <param name="nuc_file" value="demo_nucs_trailing_stop.fasta" /> + <param name="table" value="11" /> + <output name="out_nuc_align" file="demo_nuc_align.fasta" /> + </test> + </tests> + <help> +**What it does** + +Takes an input file of aligned protein sequences (typically FASTA or Clustal +format), and a matching file of unaligned nucleotide sequences (FASTA format, +using the same identifiers), and threads the nucleotide sequences onto the +protein alignment to produce a codon aware nucleotide alignment - which can +be viewed as a back translation. + +If you specify one of the standard NCBI genetic codes (recommended), then the +translation is verified. This will allow fuzzy matching if stop codons in the +protein sequence have been reprented as X, and will allow for a trailing stop +codon present in the nucleotide sequences but not the protein. + +Note - the protein and nucleotide sequences must use the same identifers. + +Note - If no translation table is specified, the provided nucleotide sequences +should be exactly three times the length of the protein sequences (exluding the gaps). + +Note - the nucleotide FASTA file may contain extra sequences not in the +protein alignment, they will be ignored. This can be useful if for example +you have a nucleotide FASTA file containing all the genes in an organism, +while the protein alignment is for a specific gene family. + +**Example** + +Given this protein alignment in FASTA format:: + + >Alpha + DEER + >Beta + DE-R + >Gamma + D--R + +and this matching unaligned nucleotide FASTA file:: + + >Alpha + GATGAGGAACGA + >Beta + GATGAGCGU + >Gamma + GATCGG + +the tool would return this nucleotide alignment:: + + >Alpha + GATGAGGAACGA + >Beta + GATGAG---CGU + >Gamma + GAT------CGG + +Notice that all the gaps are multiples of three in length. + + +**Citation** + +This tool uses Biopython, so if you use this Galaxy tool in work leading to a +scientific publication please cite the following paper: + +Cock et al (2009). Biopython: freely available Python tools for computational +molecular biology and bioinformatics. Bioinformatics 25(11) 1422-3. +http://dx.doi.org/10.1093/bioinformatics/btp163 pmid:19304878. + +This tool is available to install into other Galaxy Instances via the Galaxy +Tool Shed at http://toolshed.g2.bx.psu.edu/view/peterjc/align_back_trans + </help> +</tool> |
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| diff -r 000000000000 -r 0c24e4e2177d tools/align_back_trans/tool_dependencies.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tools/align_back_trans/tool_dependencies.xml Thu Mar 06 08:58:13 2014 -0500 |
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| @@ -0,0 +1,6 @@ +<?xml version="1.0"?> +<tool_dependency> + <package name="biopython" version="1.63"> + <repository changeset_revision="a5c49b83e983" name="package_biopython_1_63" owner="biopython" toolshed="http://toolshed.g2.bx.psu.edu" /> + </package> +</tool_dependency> |