| Previous changeset 0:bfa132aacee6 (2016-01-25) Next changeset 2:fcb4e6e95544 (2016-02-18) |
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Commit message:
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit fef8b344925620444d93d8159c0b2731a5777920 |
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modified:
deepTools_macros.xml plotCorrelation.xml readme.rst test-data/bamCompare_result2.bw test-data/bamCoverage_result1.bw test-data/bamCoverage_result2.bw test-data/bamCoverage_result4.bw test-data/bamPEFragmentSize_result1.txt test-data/bigwigCompare_result1.bw test-data/computeMatrix2.bed test-data/computeMatrix2.bw test-data/correctGCBias_result1.bam test-data/plotCoverage_result1.png test-data/plotCoverage_result1.tabular test-data/sequence.2bit test-data/test.bw tool-data/deepTools_seqs.loc.sample tool_dependencies.xml |
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added:
deepTools_macros.xml.orig static/images/GC_bias_simulated_reads_2L.png static/images/QC_plotCoverage.png static/images/bamCompare_output.png static/images/bamCoverage_output.png static/images/bamPEFragmentSize_output.png static/images/bigwigCompare_output.png static/images/computeGCBias_output.png static/images/computeMatrix_advancedOutput.png static/images/computeMatrix_output.png static/images/computeMatrix_overview.png static/images/computeMatrix_selectRegions.png static/images/computeMatrix_selectScores.png static/images/multiBamSummary_output.png static/images/multiBigwigSummary_output.png static/images/plotCorrelate_RNAseq.png static/images/plotCorrelation_output.png static/images/plotCorrelation_scatterplot_PearsonCorr_bigwigScores.png static/images/plotCoverage_annotated.png static/images/plotCoverage_output.png static/images/plotFingerprint_output.png static/images/plotHeatmap_example.png static/images/plotHeatmap_example02.png static/images/plotPCA_annotated.png static/images/plotProfiler_examples.png tool_dependencies.xml.orig |
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static/images/flowChart_computeMatrixetc.png |
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| diff -r bfa132aacee6 -r 0ed13872209b deepTools_macros.xml --- a/deepTools_macros.xml Mon Jan 25 20:22:46 2016 -0500 +++ b/deepTools_macros.xml Mon Feb 15 10:31:24 2016 -0500 |
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| @@ -72,12 +72,12 @@ </xml> <token name="@THREADS@">--numberOfProcessors "\${GALAXY_SLOTS:-4}"</token> - <token name="@WRAPPER_VERSION@">2.0.1</token> + <token name="@WRAPPER_VERSION@">2.1.0</token> <xml name="requirements"> <requirements> <requirement type="package" version="2.7.10">python</requirement> <requirement type="binary">@BINARY@</requirement> - <requirement type="package" version="2.0.1">deepTools</requirement> + <requirement type="package" version="2.1.0">deepTools</requirement> <yield /> </requirements> <expand macro="stdio" /> @@ -104,6 +104,7 @@ <param name="clustering_options" type="select" label="Clustering algorithm"> <option value="none">No clustering</option> <option value="kmeans">Kmeans clustering</option> + <option value="hclust">Hierarchical clustering</option> </param> <when value="kmeans"> <param name="k_kmeans" type="integer" value="0" label="Number of clusters to compute" @@ -112,6 +113,15 @@ If more specific clustering methods are required it is advisable to save the underlying matrix and run the clustering using other software."/> </when> + <when value="hclust"> + <param name="n_hclust" type="integer" value="0" label="number of clusters to compute." + help="WARNING: This option causes the tool to run for a very long time! When this option is + set, the matrix is split into clusters using the hierarchical clustering algorithm, using ward linkage. + This only works for data that is not grouped, otherwise only the first group will be clustered. + Note that you must have used the '--missingDataAsZero' option within computeMatrix! + If more specific clustering methods are required it is advisable to save the underlying matrix and + run the clustering using other software."/> + </when> <when value="none" /> </conditional> </when> @@ -126,6 +136,11 @@ --kmeans $advancedOpt.used_multiple_regions.clustering.k_kmeans #end if #end if + #if $advancedOpt.used_multiple_regions.clustering.clustering_options == 'hclust': + #if int($advancedOpt.used_multiple_regions.clustering.n_hclust) > 0: + --hclust $advancedOpt.used_multiple_regions.clustering.n_hclust + #end if + #end if #end if </token> @@ -293,6 +308,12 @@ </xml> <xml name="multiple_input_bams"> + <param argument="--bamfiles" type="data" format="bam" min="1" + label="Bam file" multiple="true" + help="The BAM file must be sorted."/> + </xml> + + <xml name="multiple_input_bams_min2"> <param argument="--bamfiles" type="data" format="bam" min="2" label="Bam file" multiple="true" help="The BAM file must be sorted."/> @@ -310,6 +331,7 @@ help="Title of the plot, to be printed on top of the generated image." /> </xml> + <token name="@multiple_input_bams@"> <![CDATA[ #set files=[] |
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| diff -r bfa132aacee6 -r 0ed13872209b deepTools_macros.xml.orig --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/deepTools_macros.xml.orig Mon Feb 15 10:31:24 2016 -0500 |
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| b'@@ -0,0 +1,639 @@\n+<macros>\n+\n+ <xml name="advancedOpt_scaffold">\n+ <conditional name="advancedOpt">\n+ <param name="showAdvancedOpt" type="select" label="Show advanced options" >\n+ <option value="no" selected="true">no</option>\n+ <option value="yes">yes</option>\n+ </param>\n+ <when value="no" />\n+ <when value="yes">\n+ <yield/>\n+ </when>\n+ </conditional>\n+ </xml>\n+\n+ <token name="@ADVANCED_OPTS_READ_PROCESSING@">\n+ #if $advancedOpt.doExtendCustom.doExtend == \'custom\':\n+ --extendReads $advancedOpt.doExtendCustom.extendReadsValue\n+ #else if $advancedOpt.doExtendCustom.doExtend == \'yes\':\n+ --extendReads\n+ #end if\n+ $advancedOpt.ignoreDuplicates\n+ $advancedOpt.centerReads\n+ #if $advancedOpt.minMappingQuality:\n+ --minMappingQuality \'$advancedOpt.minMappingQuality\'\n+ #end if\n+ #if $advancedOpt.samFlagInclude:\n+ --samFlagInclude $advancedOpt.samFlagInclude\n+ #end if\n+ #if $advancedOpt.samFlagExclude:\n+ --samFlagExclude $advancedOpt.samFlagExclude\n+ #end if\n+ </token>\n+\n+ <xml name="heatmap_options">\n+ <expand macro="zMin_zMax" />\n+ <expand macro="colorMap" />\n+ <expand macro="plotTitle" />\n+ <expand macro="plotNumbers" />\n+ </xml>\n+\n+ <token name="@HEATMAP_OPTIONS@">\n+ #if str($plotting_type.zMin) != "":\n+ --zMin $plotting_type.zMin\n+ #end if\n+ #if str($plotting_type.zMax) != "":\n+ --zMax $plotting_type.zMax\n+ #end if\n+ --colorMap \'$plotting_type.colorMap\'\n+ $plotting_type.plotNumbers\n+ --plotTitle \'$plotting_type.plotTitle\'\n+ </token>\n+\n+\n+ <xml name="includeZeros">\n+ <param argument="--includeZeros" type="boolean" truevalue="--includeZeros" falsevalue=""\n+ label="Include zeros"\n+ help="If set, then regions with zero counts for *all* BAM files are included. The default behavior is to ignore such regions." />\n+ </xml>\n+\n+ <xml name="zMin_zMax">\n+ <param argument="--zMin" type="float" value="" optional="true" label="Minimum value for the heatmap intensities"\n+ help="If not specified the value is set automatically."/>\n+ <param argument="--zMax" type="float" value="" optional="true" label="Maximum value for the heatmap intensities"\n+ help="If not specified the value is set automatically."/>\n+ </xml>\n+\n+ <xml name="region_limit_operation">\n+ <param argument="--region" type="text" value=""\n+ label="Region of the genome to limit the operation to"\n+ help="This is useful when testing parameters to reduce the time required. The format is chr:start:end, for example "chr10" or "chr10:456700:891000"." />\n+ </xml>\n+\n+ <token name="@THREADS@">--numberOfProcessors "\\${GALAXY_SLOTS:-4}"</token>\n+<<<<<<< HEAD\n+ <token name="@WRAPPER_VERSION@">2.0.1.0</token>\n+=======\n+ <token name="@WRAPPER_VERSION@">2.1.0</token>\n+>>>>>>> d56067f86089e75354a2fad266002db3730766d9\n+ <xml name="requirements">\n+ <requirements>\n+ <requirement type="package" version="2.7.10">python</requirement>\n+ <requirement type="binary">@BINARY@</requirement>\n+<<<<<<< HEAD\n+ <requirement type="package" version="2.0.1">deepTools</requirement>\n+=======\n+ <requirement type="package" version="2.1.0">deepTools</requirement>\n+>>>>>>> d56067f86089e75354a2fad266002db3730766d9\n+ <yield />\n+ </requirements>\n+ <expand macro="stdio" />\n+ <version_command>@BINARY@ --version</version_command>\n+ </xml>\n+\n+ <xml name="smoothLength">\n+ <param argument="--smoothLength" type="integer" value="" optional="True" min="1"\n+ label="Smooth values using the following length (in bases)"\n+ help '..b'k2 reversed</option>\n+ <option value="Reds_r">Reds reversed</option>\n+ <option value="Oranges_r">Oranges reversed</option>\n+ <option value="Greens_r">Greens reversed</option>\n+ <option value="Blues_r">Blues reversed</option>\n+ <option value="Greys_r">Greys reversed</option>\n+ <option value="Purples_r">Purples reversed</option>\n+ <option value="Paired_r">Paired reversed</option>\n+ <option value="Pastel1_r">Pastel1 reversed</option>\n+ <option value="Pastel2_r">Pastel2 reversed</option>\n+ <option value="spring_r">spring reversed</option>\n+ <option value="summer_r">summer reversed</option>\n+ <option value="autumn_r">autumn reversed</option>\n+ <option value="winter_r">winter reversed</option>\n+ <option value="hot_r">hot reversed</option>\n+ <option value="coolwarm_r">coolwarm reversed</option>\n+ <option value="cool_r">cool reversed</option>\n+ <option value="seismic_r">seismic reversed</option>\n+ <option value="terrain_r">terrain reversed</option>\n+ <option value="ocean_r">ocean reversed</option>\n+ <option value="rainbow_r">rainbow reversed</option>\n+ <option value="bone_r">bone reversed</option>\n+ <option value="flag_r">flag reversed</option>\n+ <option value="prism_r">prism reversed</option>\n+ <option value="cubehelix_r">cubehelix reversed</option>\n+ <option value="binary_r">binary reversed</option>\n+ <option value="pink_r">pink reversed</option>\n+ <option value="gray_r">gray reversed</option>\n+ <option value="copper_r">copper reversed</option>\n+ <option value="BrBG_r">BrBG reversed</option>\n+ <option value="BuGn_r">BuGn reversed</option>\n+ <option value="BuPu_r">BuPu reversed</option>\n+ <option value="GnBu_r">GnBu reversed</option>\n+ <option value="OrRd_r">OrRd reversed</option>\n+ <option value="PiYG_r">PiYG reversed</option>\n+ <option value="PRGn_r">PRGn reversed</option>\n+ <option value="PuOr_r">PuOr reversed</option>\n+ <option value="PuRd_r">PuRd reversed</option>\n+ <option value="PuBu_r">PuBu reversed</option>\n+ <option value="RdBu_r">RdBu reversed</option>\n+ <option value="RdGy_r">RdGy reversed</option>\n+ <option value="RdPu_r">RdPu reversed</option>\n+ <option value="YlGn_r">YlGn reversed</option>\n+ <option value="PuBuGn_r">PuBuGn reversed</option>\n+ <option value="RdYlBu_r">RdYlBu reversed</option>\n+ <option value="RdYlGn_r">RdYlGn reversed</option>\n+ <option value="YlGnBu_r">YlGnBu reversed</option>\n+ <option value="YlOrBr_r">YlOrBr reversed</option>\n+ <option value="YlOrRd_r">YlOrRd reversed</option>\n+ <option value="gist_gray_r">gist_gray reversed</option>\n+ <option value="gist_stern_r">gist_stern reversed</option>\n+ <option value="gist_earth_r">gist_earth reversed</option>\n+ <option value="gist_yarg_r">gist_yarg reversed</option>\n+ <option value="gist_ncar_r">gist_ncar reversed</option>\n+ <option value="gist_rainbow_r">gist_rainbow reversed</option>\n+ <option value="gist_heat_r">gist_heat reversed</option>\n+ <option value="gnuplot_r">gnuplot reversed</option>\n+ <option value="gnuplot2_r">gnuplot2 reversed</option>\n+ <option value="CMRmap_r">CMRmap reversed</option>\n+ <option value="bwr_r">bwr reversed</option>\n+ <option value="hsv_r">hsv reversed</option>\n+ <option value="brg_r">brg reversed</option>\n+ <option value="jet_r">jet reversed</option>\n+ <option value="afmhot_r">afmhot reversed</option>\n+ </param>\n+\n+ </xml>\n+\n+</macros>\n' |
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| diff -r bfa132aacee6 -r 0ed13872209b plotCorrelation.xml --- a/plotCorrelation.xml Mon Jan 25 20:22:46 2016 -0500 +++ b/plotCorrelation.xml Mon Feb 15 10:31:24 2016 -0500 |
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| @@ -1,5 +1,5 @@ <tool id="deeptools_plot_correlation" name="plotCorrelation" version="@WRAPPER_VERSION@.0"> - <description>creates a heatmap or scatterplot of correlation scores between different samples </description> + <description>Create a heatmap or scatterplot of correlation scores between different samples </description> <macros> <token name="@BINARY@">plotCorrelation</token> <import>deepTools_macros.xml</import> @@ -88,34 +88,51 @@ </tests> <help> <![CDATA[ -**What it does** +What it does +-------------- + +This tools takes the default output of ``multiBamSummary`` or ``multiBigwigSummary``, and computes the pairwise correlation among samples. +Results can be visualized as **scatterplots** or as +a **heatmap** of correlation coefficients (see below for examples). -This tools takes a compressed matrix of scores (such as read coverages) for a number of genomic regions -and different samples. It can visualize the correlation among samples as scatterplots or as -a heatmap of correlation coefficients. Further output files are optional. -The compressed input matrices are easily generated using the "multiBamSummary" and "multiBigwigSummary" tools. +Background +------------ + +The result of the correlation computation is a **table of correlation coefficients** that indicates how "strong" the relationship between two samples is and it will consist of numbers between -1 and 1. (-1 indicates perfect anti-correlation, 1 perfect correlation.) +We offer two different functions for the correlation computation: *Pearson* or *Spearman*. -.. image:: $PATH_TO_IMAGES/QC_multiBamSummary_humanSamples.png - :alt: Heatmap of RNA Polymerase II ChIP-seq - +The *Pearson method* measures the **metric differences** between samples and is therefore influenced by outliers. +The *Spearman method* is based on **rankings**. -You can find more details on plotCorrelation here http://deeptools.readthedocs.org/en/master/content/tools/plotCorrelation.html +Output +-------- +The default output is a **diagnostic plot** -- either a scatterplot or a clustered heatmap displaying the values for each pair-wise correlation (see below for example plots). -**Output files**: +Optionally, you can also obtain a table of the pairwise correlation coefficients. -- **diagnostic plot**: Either a scatterplot or clustered heatmap (select above) displaying the values for each pair-wise correlation, - see below for an example -- data matrix (optional): if you want to analyze or plot the correlation values using a different program, e.g. R, this matrix can be used +.. image:: $PATH_TO_IMAGES/plotCorrelation_output.png + :width: 600 + :height: 271 -**Output with test dataset**: +Example plots +-------------- -The following is the output of plotCorrelation with our test ChIP-Seq datasets. Average coverages were computed over 10kb bins for chromosome X, -from bigwig files using multiBigwigSummary. The output was used by plotCorrelation to make a heatmap of spearman correlation between samples. +The following is the output of ``plotCorrelation`` with our test ChIP-Seq datasets (to be found under "Shared Data" --> "Data Library"). + +Average coverages were computed over 10 kb bins for chromosome X, +from bigWig files using ``multiBigwigSummary``. This was then used with ``plotCorrelation`` to make a heatmap of Spearman correlation coefficients. .. image:: $PATH_TO_IMAGES/plotCorrelation_galaxy_bw_heatmap_output.png + :width: 600 + :height: 518 +The scatterplot could look like this: + +.. image:: $PATH_TO_IMAGES/plotCorrelation_scatterplot_PearsonCorr_bigwigScores.png + :width: 600 + :height: 600 ----- |
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| diff -r bfa132aacee6 -r 0ed13872209b test-data/bamPEFragmentSize_result1.txt --- a/test-data/bamPEFragmentSize_result1.txt Mon Jan 25 20:22:46 2016 -0500 +++ b/test-data/bamPEFragmentSize_result1.txt Mon Feb 15 10:31:24 2016 -0500 |
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| @@ -2,19 +2,19 @@ Fragment lengths: -Min.: 241 +Min.: 241.0 1st Qu.: 241.5 Mean: 244.666666667 Median: 242.0 3rd Qu.: 246.5 -Max.: 251 +Max.: 251.0 Std: 4.49691252108 Read lengths: -Min.: 251 +Min.: 251.0 1st Qu.: 251.0 Mean: 251.0 Median: 251.0 3rd Qu.: 251.0 -Max.: 251 +Max.: 251.0 Std: 0.0 |
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| diff -r bfa132aacee6 -r 0ed13872209b test-data/plotCoverage_result1.tabular --- a/test-data/plotCoverage_result1.tabular Mon Jan 25 20:22:46 2016 -0500 +++ b/test-data/plotCoverage_result1.tabular Mon Feb 15 10:31:24 2016 -0500 |
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| b"@@ -1,16570 +1,16570 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t0.0\t0.0\n+chrM\t16565\t16566\t0.0\t0.0\n+chrM\t16566\t16567\t0.0\t0.0\n+chrM\t16567\t16568\t0.0\t0.0\n+chrM\t16568\t16569\t0.0\t0.0\n' |
| b |
| diff -r bfa132aacee6 -r 0ed13872209b tool_dependencies.xml --- a/tool_dependencies.xml Mon Jan 25 20:22:46 2016 -0500 +++ b/tool_dependencies.xml Mon Feb 15 10:31:24 2016 -0500 |
| b |
| @@ -1,9 +1,9 @@ <?xml version="1.0"?> <tool_dependency> <package name="python" version="2.7.10"> - <repository changeset_revision="0339c4a9b87b" name="package_python_2_7_10" owner="iuc" toolshed="https://toolshed.g2.bx.psu.edu" /> + <repository changeset_revision="0339c4a9b87b" name="package_python_2_7_10" owner="iuc" prior_installation_required="True" toolshed="https://toolshed.g2.bx.psu.edu" /> </package> - <package name="deepTools" version="2.0.1"> - <repository changeset_revision="1fb61fa32733" name="package_python_2_7_deeptools_2_0_1" owner="iuc" toolshed="https://toolshed.g2.bx.psu.edu" /> + <package name="deepTools" version="2.1.0"> + <repository changeset_revision="2c54c7c38299" name="package_python_2_7_deeptools_2_1_0" owner="iuc" toolshed="https://toolshed.g2.bx.psu.edu" /> </package> </tool_dependency> |
| b |
| diff -r bfa132aacee6 -r 0ed13872209b tool_dependencies.xml.orig --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool_dependencies.xml.orig Mon Feb 15 10:31:24 2016 -0500 |
| [ |
| @@ -0,0 +1,76 @@ +<?xml version='1.0' encoding='utf-8'?> +<tool_dependency> + <package name="numpy" version="1.9"> + <repository name="package_python_2_7_numpy_1_9" owner="iuc" prior_installation_required="True" /> + </package> + <package name="pybigwig" version="0.2.4"> + <repository name="package_python_2_7_10_pybigwig_0_2_4" owner="iuc" prior_installation_required="True"/> + </package> + <package name="matplotlib" version="1.4"> + <repository name="package_python_2_7_matplotlib_1_4" owner="iuc" prior_installation_required="True" /> + </package> + <package name="scipy" version="0.14"> + <repository name="package_python_2_7_scipy_0_14" owner="iuc" prior_installation_required="True" /> + </package> +<<<<<<< HEAD + <package name="deepTools" version="2.0.1"> + <repository name="package_python_2_7_deeptools_2_0_1" owner="iuc"/> +======= + <package name="pysam" version="0.8.3"> + <repository name="package_python_2_7_pysam_0_8_3" owner="iuc" prior_installation_required="True" /> + </package> + <package name="bx-python" version="0.7.2"> + <repository name="package_python_2_7_bx_python_0_7" owner="iuc" prior_installation_required="True" /> + </package> + <package name="python" version="2.7.10"> + <repository name="package_python_2_7_10" owner="iuc" prior_installation_required="True" /> +>>>>>>> d56067f86089e75354a2fad266002db3730766d9 + </package> + <package name="deepTools" version="2.1.0"> + <install version="1.0"> + <actions> + <action type="setup_python_environment"> + <repository name="package_python_2_7_10" owner="iuc"> + <package name="python" version="2.7.10" /> + </repository> + <repository name="package_python_2_7_pysam_0_8_3" owner="iuc"> + <package name="pysam" version="0.8.3" /> + </repository> + <repository name="package_python_2_7_numpy_1_9" owner="iuc"> + <package name="numpy" version="1.9" /> + </repository> + <repository name="package_python_2_7_matplotlib_1_4" owner="iuc"> + <package name="matplotlib" version="1.4" /> + </repository> + <repository name="package_python_2_7_scipy_0_14" owner="iuc"> + <package name="scipy" version="0.14" /> + </repository> + <repository name="package_python_2_7_bx_python_0_7" owner="iuc"> + <package name="bx-python" version="0.7.2" /> + </repository> + <repository name="package_python_2_7_10_pybigwig_0_2_4" owner="iuc"> + <package name="pybigwig" version="0.2.4" /> + </repository> + <package sha256sum="3bf835afde02cd5247198db3e01f690899e1fdb86e1821a83ac940d807baa857">https://pypi.python.org/packages/source/d/deepTools/deepTools-2.1.0.tar.gz</package> + </action> + + <action type="set_environment"> + <environment_variable name="PATH" action="prepend_to">$INSTALL_DIR/bin</environment_variable> + <environment_variable name="PYTHONPATH" action="prepend_to">$INSTALL_DIR</environment_variable> + <environment_variable name="DEEPTOOLS_ROOT_PATH" action="set_to">$INSTALL_DIR</environment_variable> + <environment_variable name="DEEPTOOLS_PYTHONPATH" action="set_to">$INSTALL_DIR</environment_variable> + <environment_variable name="DEEPTOOLS_PATH" action="set_to">$INSTALL_DIR/bin</environment_variable> + <!-- libpng lib path --> + <environment_variable action="set_to" name="LIBPNG_LIB_PATH">$ENV[LIBPNG_LIB_PATH]</environment_variable> + <environment_variable action="prepend_to" name="LD_LIBRARY_PATH">$ENV[LIBPNG_LIB_PATH]</environment_variable> + <!-- disable the config file of deepTools --> + <environment_variable name="DEEP_TOOLS_NO_CONFIG" action="set_to">TRUE</environment_variable> + </action> + </actions> + </install> + <readme> + Installation of deepTools from Fidel Ramirez. + https://github.com/fidelram/deepTools + </readme> + </package> +</tool_dependency> |
