| Next changeset 1:0632744d5a5f (2020-05-23) |
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Commit message:
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/medaka commit 28167363fc58681984e067445dd9c6a25fd33a6c" |
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added:
medaka.xml test-data/medaka/consensus.fasta test-data/plasmid.fasta test-data/reads.fastq |
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| diff -r 000000000000 -r 1c4402b9244d medaka.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/medaka.xml Sun Feb 23 09:04:41 2020 -0500 |
| [ |
| @@ -0,0 +1,69 @@ +<tool id="medaka_consensus" name="medaka_consensus" version="@VERSION@" profile="19.01"> + <description> creates a consensus sequence from nanopore sequencing data</description> + <macros> + <token name="@VERSION@">0.11.5</token> + </macros> + <requirements> + <requirement type="package" version="@VERSION@">medaka</requirement> + </requirements> + <version_command>medaka --version</version_command> + <command detect_errors="exit_code"><![CDATA[ + medaka_consensus + -i '${basecalled}' + -d '${draft}' + -o out_dir + -t \${GALAXY_SLOTS:-4} + -m '${model}' > log.txt + ]]> </command> + + <inputs> + <param name="basecalled" type="data" format="fastq,fastq.gz,fastqsanger,fastqsanger.gz,fasta,fasta.gz" + label="Basecalled data"/> + <param name="draft" type="data" format="fasta,fasta.gz" label="Draft assemblies" + help="The input assembly should be preprocessed with racon"/> + <param name="model" type="select" label="Model"> + <option value="r941_min_fast_g303">r941_min_fast_g303</option> + <option value="r941_min_high_g303" selected="True">r941_min_high_g303</option> + <option value="r941_min_high_g330">r941_min_high_g330</option> + <option value="r941_min_high_g344">r941_min_high_g344</option> + <option value="r941_prom_fast_g303">r941_prom_fast_g303</option> + <option value="r941_prom_high_g303">r941_prom_high_g303</option> + <option value="r941_prom_high_g344">r941_prom_high_g344</option> + <option value="r941_prom_high_g330">r941_prom_high_g330</option> + <option value="r10_min_high_g303">r10_min_high_g303</option> + <option value="r10_min_high_g340">r10_min_high_g340</option> + <option value="r103_min_high_g345">r103_min_high_g345</option> + <option value="r941_prom_snp_g303">r941_prom_snp_g303</option> + <option value="r941_prom_variant_g303">r941_prom_variant_g303</option> + <option value="r941_min_high_g340_rle">r941_min_high_g340_rle</option> + </param> + </inputs> + <outputs> + <!-- <collection name="list_output" type="list" label="Output"> + <discover_datasets pattern="__name_and_ext__" directory="out_dir"/> + </collection> --> + <data name="consensus" format="fasta" label="${tool.name} on ${on_string}: consensus" from_work_dir="out_dir/consensus.fasta"/> + <data name="log" format="txt" label="${tool.name} on ${on_string}: Log" from_work_dir="log.txt"/> + </outputs> + <tests> + <test> + <param name="draft" value="plasmid.fasta" /> + <param name="basecalled" value="reads.fastq" /> + <param name="model" value="r941_min_fast_g303"/> + <output name="consensus" file="medaka/consensus.fasta" ftype="fasta" /> + </test> + </tests> + <help><![CDATA[ + +.. class:: infomark + +**What it does** + +medaka is a tool to create a consensus sequence from nanopore sequencing data. +This task is performed using neural networks applied from a pileup of individual sequencing +reads against a draft assembly. It outperforms graph-based methods operating on basecalled data, +and can be competitive with state-of-the-art signal-based methods, whilst being much faster. + + + ]]></help> +</tool> |
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| diff -r 000000000000 -r 1c4402b9244d test-data/medaka/consensus.fasta --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/medaka/consensus.fasta Sun Feb 23 09:04:41 2020 -0500 |
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| @@ -0,0 +1,2 @@ +>4_segment0 4:1.0-3792.0 +TTGCGGTGTCTGCGTCAGAATCGCGTTCAGCGCGTTTCAGCGGTGCGTACAATTACAGCATTATGTTAAATTTTATAATTGTCTTTAGTCATTGCGCTATGATTTTGTAGGTGGAGTTGTTTCATATTTTTGATATAAATTCTTTTATCTCACTGAAGCAGTCATCGTCAGTATCAATGGTTTTGTTCTTCATTGATATGCCTATTCTAGAATCTGATTCCTTATTACAACTAAGGTAAACTTGAACTTTTGCTTTCTTTTCATTATTTGGGTAAGCGTAATCAGGATCTAAGGATGTAATAATAGATTCTTGTTCTTTTATTTTATTGAATAGATCGTCTATATATCTAAACACCTCTTTCTTACACGCGTGAGCAATGATTAGATCTTCTATTGCGCCATTCTTGTTTTTTCCTGGCATGATAAAGAAAGATATTTTTGTGCTTCCGTTAATGGCTATTTCATTATGCGAGCTTGGTGATGAAATGTTAAGCTCATTTAATGTTGAAATTATCGATCTTTCCGTGTCAGAAAACGATTCGTCGGCATCGATGATTAAGAATATACTTTCTTTTTCATTAAAAGACTCTATGCTTTTAAATGCTCTAAGGGTTTGTTTTAGTTTTGTCTTTCCATTTATTTTTTCAACATAAACATCATTAATTCCTTCTATTTCAAGTAGATTGCAAAGGAAGTGAAAATCATCATCACCCTCAACGAAAATGACTTTTTTATAATCATTTATTTTTCTCGGTTTCATTTATCTCACCTCCCAGCCATTTTCAACTGAAAAAGAAAAATCATCCATGTTAAATTGCATTGCTGTAGGTTTTTGAGATTCCTTGTCTCTTCCCAGTCTTATGTAAGATATATCTTTTCTTTTTTCATCTAATTCGTTTATCGCACATATTACATCATGGCTGTGTGTTGTTGTAAATATTTGATTATTATTGGTTTTTGCTGCTTCTATCAAAGCGTTGATCATATCTTTGATTACTGAGTAATGAATTCCATTTTCTATCTCATCAATGAGAATAATTGAGTCTTTTTGAACTAAGATTGAGCTAATGAAAGACAGCGCTCTGCTTACTCCTTCACCAAGCATTGATATTTCATTTAATTCACTTAGTCCTATATCTAAAAAAATCTCTTTGTTTTGATCTGTTGCTATTATTGCAATATCGTTAATTCTATTATCTATTCTTTTTAGGTTGCCTATTATTTCATCTTTTCTTTTTTGTGTTAGTAGTTTTGATACGTTTTTTATCGTTGCTTCTTTGTTTATGTTTCTTGATGTGGTTATTATCGCTGCGGTTTTGAATTCATATAGCTTACCTTTTTTAGTTTGTTTTATTTCTCCGTTTATTTCTTGTGCTTTTTGTGTTATTTTTATAGTAATCAATTCTTCTGGGGGGTTCTTTTTCCGTTCTGTGTTTCACTAACCTTAATCATTAAAACATTCCCCTTTTCCTGAGAGCCGAGGGTTTGTCGCAATGGAGGGGTGTTTGATAATATTGACTCAACATTGAGGGACACGCTTGATTCACTTTGTGTGTTATCTATCGTTGAGTATTTTTGTGTTACTTCTGACCTTTCATCTCTTATTGTTATGGACATTTCTTTGGATGTGTCCATGTTATAAAAATAGCTATCCCAAAATGAGTTTTCTTTATTTAAAAGAGATGCTTCATTTCTAAAGCTTTTTACTTTTAACAGAGCTGCTGGGTTTTTTACGTCATAAAATGAAAATATCGCGTCTAATATCGATGTTTTCCCAAAGTTGTTTTTTCCGGATATTACATTAAGTGTTTTTAGTTGTTTGAGAGTTATGCTCTCGAAACACTTAAAATTTTTAAGTGGATATCTTTTATCATGTTTAAACTTGCTCTCCATTTCTGTTTACGTTTACATAGTAATAATGTGTTTATTTATGCATCACTATGCCACTGTTTCTTTTCTAGAAGTATAATCAAAATAATATTACTTTATAGTCCTGTTTGGATTGATTTTAACCAGTTTGTCGGAGTTTCTTCTTGTGATTATTGTGTTCTGGTCGTAATCTCCTAAACTGAAAACGAAAAAACCACCTGGGGAGGTGGTTTGATCGAAGGTTAAGTCAGTTGGGGAACTGCTTAACCAGGTAACTGTCTTGTGCGAGACGAGTCACCAAAACTGTCCTTTCAGTGTAGCTGTAGTCAGGCCATCACTTCAAGTACTCTCTTGAACCTCTCGCACATCGACTTTTACCAATGGCTGCTGCCAGTGGCGCTTTGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACCGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACACCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGGCTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGCGGAGCCTATGGAAAAACGCCTGCGGCGCTGGCTTCCTCCGGTGCTTTGCTTTTTGCTCACATGTTCTTTCCGACTTTATCCCCCGATTCTGTGGATAACCGTATTACCGCTTTTGAGTGAGCTGACACCGCTCGCCGCAGTCGAACGACCGAGCGTAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTTATGTGACATTTTCTCCTTACGCTCTGTTGTGCCGTTCGGCATCCTGTCCTGAGCGTTATCTCTCTGTGCTATTTTCTACTTCAAAGCGTGTCTGGATGCTGTTCTGGAGTTCTTCTGCGAGTTCGTGCAGCTTCTCACACATGGCAGCCTGTTCGTCAGCATCGGGTGCGTCCAGTTTTTCGAGCAGCGTCAGGCTCTGACTTTTTATGAATCCCGCCATGTTGAGTACGGCTTGCTGCTGCTTGTTCATCTTTTCGTTTTCTCCGTTCTGTCTGTCATCTGCGTTGTGTGATTATATCGCGTACCACTTTTCGACTGTTTTGCTGCCGTTATTCTGCCGCTTGGCTTTTTGACGGGCATTTCTGTCAGACAACACTGTCACTGCCAAAAAACTGCCGTGCCTTTGTCGGTAATTCGAGCTTGCTGACAGGACAGGATGTGCAATTGTTATACCGCGCATACATGCACGCTATTACAATTGCCCTGGTCAGGGCTTTGCCCCGACACCCATGTCAGATACGGAGCCATGTTTTATGACAAAACGAAGTGGAAGTAATACGCGCAGGCGGGCTATCAGTCGCCCTGTTCGTCTGACGGCAGAAGAAGACCAGGAAATCAGAAAAAGGGCTGCTGAATGCGGCAAGACCGTTTCCGGTTTTTTACGGGCGGCAGCTCTCGGTAAGAAAGTTAACTCACTGACTGATGATCGGGTACTGAAAGAAGTTATGAGACTGGGGGCGTTACAGAAAAAACTCTTTATCGACGGCAAGCGTGTCGGGGACAGGGAGTATGCGGAGGTGCTGATCGCTATTACGGAGTATCACCGTGCCCTGTTATCCAGACTTATGGCAGATTAGCTTCCCGGAGAGAAAACTGTCGAAAACAGACGGTATGAACACCGTAAGCTCCCAAAGTGATCACCATTCGCTTTCATGCATAGCTATGCAGCGAGCTGAAAACGATCCTGACGCATCCTTCCTGTTTTCCCGGGGTAAAACATCTCTTT |
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| diff -r 000000000000 -r 1c4402b9244d test-data/plasmid.fasta --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/plasmid.fasta Sun Feb 23 09:04:41 2020 -0500 |
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| @@ -0,0 +1,56 @@ +>4 length=3792 depth=114.52x circular=true +TTGCGGTGTCTGCGTCAGAATCGCGTTCAGCGCGTTTCAGCGGTGCGTACAATTACAGCATTATGTTAAA +TTTTATAATTGTCTTTAGTCATTGCGCTATGATTTTGTAGGTGGAGTTGTTTCATATTTTTGATATAAAT +TCTTTTATCTCACTGAAGCAGTCATCGTCAGTATCAATGGTTTTGTTCTTCATTGATATGCCTATTCTAG +AATCTGATTCCTTATTACAACTAAGGTAAACTTGAACTTTTGCTTTCTTTTCATTATTTGGGTAAGCGTA +ATCAGGATCTAAGGATGTAATAATAGATTCTTGTTCTTTTATTTTATTGAATAGATCGTCTATATATCTA +AACACCTCTTTCTTACACGCGTGAGCAATGATTAGATCTTCTATTGCGCCATTCTTGTTTTTTCCTGGCA +TGATAAAGAAAGATATTTTTGTGCTTCCGTTAATGGCTATTTCATTATGCGAGCTTGGTGATGAAATGTT +AAGCTCATTTAATGTTGAAATTATCGATCTTTCCGTGTCAGAAAACGATTCGTCGGCATCGATGATTAAG +AATATACTTTCTTTTTCATTAAAAGACTCTATGCTTTTAAATGCTCTAAGGGTTTGTTTTAGTTTTGTCT +TTCCATTTATTTTTTCAACATAAACATCATTAATTCCTTCTATTTCAAGTAGATTGCAAAGGAAGTGAAA +ATCATCATCACCCTCAACGAAAATGACTTTTTTATAATCATTTATTTTTCTCGGTTTCATTTATCTCACC +TCCCAGCCATTTTCAACTGAAAAAGAAAAATCATCCATGTTAAATTGCATTGCTGTAGGTTTTTGAGATT +CCTTGTCTCTTCCCAGTCTTATGTAAGATATATCTTTTCTTTTTTCATCTAATTCGTTTATCGCACATAT +TACATCATGGCTGTGTGTTGTTGTAAATATTTGATTATTATTGGTTTTTGCTGCTTCTATCAAAGCGTTG +ATCATATCTTTGATTACTGAGTAATGAATTCCATTTTCTATCTCATCAATGAGAATAATTGAGTCTTTTT +GAACTAAGATTGAGCTAATGAAAGACAGCGCTCTGCTTACTCCTTCACCAAGCATTGATATTTCATTTAA +TTCACTTAGTCCTATATCTAAAAAAATCTCTTTGTTTTGATCTGTTGCTATTATTGCAATATCGTTAATT +CTATTATCTATTCTTTTTAGGTTGCCTATTATTTCATCTTTTCTTTTTTGTGTTAGTAGTTTTGATACGT +TTTTTATCGTTGCTTCTTTGTTTATGTTTCTTGATGTGGTTATTATCGCTGCGGTTTTGAATTCATATAG +CTTACCTTTTTTAGTTTGTTTTATTTCTCCGTTTATTTCTTGTGCTTTTTGTGTTATTTTTATAGTAATC +AATTCTTCTGGGGGGGTTCTTTTTCCGTTCTGTGTTTCACTAACCTTAATCATTAAAACATTCCCCTTTT +CCTGAGAGCCGAGGGTTTGTCGCAATGGAGGGGTGTTTGATAATATTGACTCAACATTGAGGGACACGCT +TGATTCACTTTGTGTGTTATCTATCGTTGAGTATTTTTGTGTTACTTCTGACCTTTCATCTCTTATTGTT +ATGGACATTTCTTTGGATGTGTCCATGTTATAAAAATAGCTATCCCAAAATGAGTTTTCTTTATTTAAAA +GAGATGCTTCATTTCTAAAGCTTTTTACTTTTAACAGAGCTGCTGGGTTTTTTACGTCATAAAATGAAAA +TATCGCGTCTAATATCGATGTTTTCCCAAAGTTGTTTTTTCCGGATATTACATTAAGTGTTTTTAGTTGT +TTGAGAGTTATGCTCTCGAAACACTTAAAATTTTTTAAGTGGATATCTTTTATCATGTTTAAACTTGCTC +TCCATTTCTGTTTACGTTTACATAGTAATAATGTGTTTATTTATGCATCACTATGCCACTGTTTCTTTTC +TAGAAGTATAATCAAAATAATATTACTTTATAGTCCTGTTTGGATTGATTTTAACCAGTTTGTCGGAGTT +TCTTCTTGTGATTATTGTGTTCTGGTCGTAATCTCCTAAACTGAAAACGAAAAAACCACCTGGGGAGGTG +GTTTGATCGAAGGTTAAGTCAGTTGGGGAACTGCTTAACCAGGTAACTGTCTTGTGCGAGACGAGTCACC +AAAACTGTCCTTTCAGTGTAGCTGTAGTCAGGCCATCACTTCAAGTACTCTCTTGAACCTCTCGCACATC +GACTTTTACCAATGGCTGCTGCCAGTGGCGCTTTGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTT +ACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACC +TACACCGAACCGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGG +ACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACACCTG +GTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGGCTTGAGCGTCGATTTTTGTGATGCTCGTCAGGG +GGGCGGAGCCTATGGAAAAACGCCTGCGGCGCTGGCTTCCTCCGGTGCTTTGCTTTTTGCTCACATGTTC +TTTCCGACTTTATCCCCCGATTCTGTGGATAACCGTATTACCGCTTTTGAGTGAGCTGACACCGCTCGCC +GCAGTCGAACGACCGAGCGTAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTTATGTGACATTTTCT +CCTTACGCTCTGTTGTGCCGTTCGGCATCCTGTCCTGAGCGTTATCTCTCTGTGCTATTTTCTACTTCAA +AGCGTGTCTGGATGCTGTTCTGGAGTTCTTCTGCGAGTTCGTGCAGCTTCTCACACATGGCAGCCTGTTC +GTCAGCATCGGGTGCGTCCAGTTTTTCGAGCAGCGTCAGGCTCTGACTTTTTATGAATCCCGCCATGTTG +AGTACGGCTTGCTGCTGCTTGTTCATCTTTTCGTTTTCTCCGTTCTGTCTGTCATCTGCGTTGTGTGATT +ATATCGCGTACCACTTTTCGACTGTTTTGCTGCCGTTATTCTGCCGCTTGGCTTTTTGACGGGCATTTCT +GTCAGACAACACTGTCACTGCCAAAAAACTGCCGTGCCTTTGTCGGTAATTCGAGCTTGCTGACAGGACA +GGATGTGCAATTGTTATACCGCGCATACATGCACGCTATTACAATTGCCCTGGTCAGGGCTTTGCCCCGA +CACCCATGTCAGATACGGAGCCATGTTTTATGACAAAACGAAGTGGAAGTAATACGCGCAGGCGGGCTAT +CAGTCGCCCTGTTCGTCTGACGGCAGAAGAAGACCAGGAAATCAGAAAAAGGGCTGCTGAATGCGGCAAG +ACCGTTTCCGGTTTTTTACGGGCGGCAGCTCTCGGTAAGAAAGTTAACTCACTGACTGATGATCGGGTAC +TGAAAGAAGTTATGAGACTGGGGGCGTTACAGAAAAAACTCTTTATCGACGGCAAGCGTGTCGGGGACAG +GGAGTATGCGGAGGTGCTGATCGCTATTACGGAGTATCACCGTGCCCTGTTATCCAGACTTATGGCAGAT +TAGCTTCCCGGAGAGAAAACTGTCGAAAACAGACGGTATGAACACCGTAAGCTCCCAAAGTGATCACCAT +TCGCTTTCATGCATAGCTATGCAGCGAGCTGAAAACGATCCTGACGCATCCTTCCTGTTTTCCCGGGGTA +AAACATCTCTTT \ No newline at end of file |
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| diff -r 000000000000 -r 1c4402b9244d test-data/reads.fastq --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/reads.fastq Sun Feb 23 09:04:41 2020 -0500 |
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| b"@@ -0,0 +1,1016 @@\n+@215f1e0c-27d1-4446-bca5-cb0fd6a8c054\n+TTGCAGTATCTGCGTCAGAATCGCATTCCAGCCGCAGGTGTTCAGCGGTGCGTACAATTACAGCATTATGTTAAATTTTATAATTGTCTTTAGTCATTGCGCTATGATTTGTAGGTGGAGTTGTTTCATATTTTGATATAAATTCTTTTATCTCACCAAACAGTCATCGTCAGTATCAATGGTTTTGTTCTTCATTGATATTACCTATTCTAGAATCTGATTCCTTGTACAACTAAGGTAAACTTGAACTTTGCTTTCTTTTCATTATTTGGGTAAGCGTAATGATGTAGGATCTAGGATATATCTATAATAATAATTCTTGTTCTTTTTTTATTTTATTGAATATGAATCAATACCTGTCTAAGCACACCTCTTTCTTACGCGTGAGCAATGGTTAGATGGATCTTCTGGCGGCCTTGCGCCATTCCTGTTTGTTC\n++\n+?B4-)&'%<ABC>:883110,;*-$/+%)(.(($$#%&$$#%14+6-/=1@5@A>GE@F@<B8B..0F>?AA13EJHTIDHBDAJB@BOM908=B;=>49/(?>>?>?@?(004?C57/9<B>DEEE@@@C?=>=<912+,*'1/1396;78+&$&,15(85::C@>BA>9(,</(B:;G?B;@K=<HF?JF5**+&&0/#%$;>BCB=(4<.=91<4@ADC9:AC$=9>555=3412;6;+=>?@>2$%+839@BH=CAOHFCI<.98<-0/*$%)0**()(')&$)$474)+/,'267432&&'&''''$%&'*')>?C3=;467===95489G:./5229,$$&$(&&$%%(''')+8%/0%.-%0$')&'*=566(-(685''57./4%)44+/*$$$'42:;6($$##$$$'-0(%67112$$'$())'7;:\n+@baf1c4f7-8f02-4c92-be5f-0431ca399c18\n+GGTATTACTTCGTTCAGTTACGTATTGCTGCGCGCACTCTCTGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACAGGGGAGTTCGTGCACACAGCCCGAAGCTTGGAGCGAACGACCTACGCGAACCGAGATACCTACAGCGTGAGCTATGAAAGCGCCACGCTTCCCGAAGGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCGGGGGAAACACACCTGGTATCTTTTTATAGTCCTGTCCGGGTTTCGCCACCTCTGGCAGGCGTCGATTTTTGTGATGCTCGTCAGAGCGGAATTTATGGAAACGCCTGCGGCGCTGGCTTCCTCCGATGCTGCTTTTTGCTCACATATTCTTTCCGACTTTATCCCCCGATTCTGTGGATAACCGTATTACCGCTGCAGGTGAGCTGACACGCTCGCCGCAGTCGAACGACCGAGAGCGTAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTATGTGACATTTTCTCCTTACGCTCTGTTGTGCCGTTCGGCATCCTGTCTGAGCGTTATCTCTCTGTGCTATTTTTACTTCAAAGCGTGTCTGGATGCTGTTCTGGAGTTCTTCTGCGAGTTCGTGCAGCTTCTCACACATGGCAGCCTGTTCGTCAGCATCGGGTGCGTCCAGTTTTCGAGCAGCATTCAGGCTCTGACTTTTATGAATCCCGCCATGTTGGTACGGCTTTTCTTTCTTGTTCATCTTTTCGTTTTCTCCGTTCTGTCTGTCATCTGCGTTGTGTATGATTATATCGCGTACCACTTTTCGGCTGTTTGCTGCCGTTATTCTGCGCCGCTTGGCTTTTTGACGGGCATTTCTGTCAGACAACACTGTCACTGCCAAAAAACTGCCGTGCCTTTGTCGGTAATTCGAGCTTGCTGACAGGACAGGATGTACAATTGTTATACCGCGCATACATGCACGCTATTACAATTGCCCTGGTCAGGAGCTTTGCCCCGACACCCATGTCCAGATACGGAGCCATGTTTTGCTGACAAAACGAAGTGGAAGTAATACGCGCAGGCGGGCTATCAGTCCCCTGTTCGTCTGACGGCAGAGAGAAGACCAGGAAATCAGAAAAAGGGCTGCTGAATGCGGCAAGACCGTTTCGGTTTTTACGGGCGGCAGCTCTCGGTAAGAAAGTTAACCCACTGACTGATGATCGGGTACTGAAAGAAGTTATGAGACTGGGAGCGTTACAGAAAAAACTCTTTATCGACGGCAAGCGTGTCGGGGACAGGAGTATGCGTTTGATCGCTATTACGGGTATCACGTGCCCTGTTATCAGACTTATGGCAGATTGGCTTCCCGGAGAGAAAACTGTCGAAAACAGACGGTATGAACACCGTAAGCTCCCAAAGTGATCACCATTCGCTTTCATGCATAGCTATGCAGCGAGCTGAAAACGATCCTGACGCATCCTTCCTGTTTTCCCGGGGTAAACATCTTTTTTGCAGTGTCTGCGTCAGAATCGCGTTCAGCGCGTTTCAGCAGTGCGTACAATTACAGCATTATGTTAAATTTTATAGTGTCTTTAGTCATTTGCGCTATGATTTTGTAGGTGGAGTTGCTTCATATTTTTGATATAAAGTTCTTTTCCCATCTGCTAGAGCAGTCATCGTCAGTATCAATGGTTTGTTCTTCATTGATATGCCTATTCTAGAATCTGGTCCTTATTACAACTAAGGTAAACTTGAACTTTTGCTTTCTTTTCATTATTTGGGTAAGCGTAATCAGGATCTAAGGATGTAATAATAGATTCTTGTTCTTTTTTATTTTATTGAATAGATCGTCTATATATCTAAACACCTCTTTCTTACACGCGTGAGCAATAGTTGGAGTCTTCTATTGCGCCATTCTTGTTTTTCCTGGCATGATAAAGAAGATATTTTTGTGCTTCCCGTTAATGGCTATTTCATTGTGCGAGCTTGGTGATAAATGTTAAGCTCATTTAATGTTGAAATTATCTGATCTTTCCGTGTCAGAAAAACGATTCCGTCGGCATCGATGATTAAGAATATACTTTCTTTTCCATTAAAGACTCTATGCTTTTAAATGCTCTAAGGGTTTGTTTTAGTTTGTCTTTCCATTTTATTTTTTCAACATAAACATCATTAATTCCTTCTATTTCAAGTAGATTTAGGAAGTGAAAATCATCATCACCCTCAACAAAAAATGACTTTTTTATAATCATTTATTTTCTCGGTTTCATTTATCTCACCTCCCAGCCATTTTCAACTGAAAAGGAAAATCATCCATGTTAAATTGCATTGCTGTAGGTTTTGGGAATTGCTTGTCTCTTCCCAGTCTTATGTAAGATATATCTTTCTTTTTCATCTAATTCGTTTATCGCACATATTACATCATGGCTGTGTGTTGTTGTAAATATTTGATTATTATTGGTTTTGCTGCTTCTATCAAAGCGTTGATCATATCTTTGATTACTGAGTAATGAATTCCATTTTCTATCTCATCAATGAGAATAATTGAGTCTTTTTGAACTAAGATTGAGCTAATGAAAGACAGCGCTCTGCTTACTCCTTCACCAAGCATTGATATTTCATTTAATTCACTTAGTCCTATATCTAAAAAAATCTCTTTGTTTTGATCTGTTGCTATTATTGCAATATCGTTAATTCTATTATCTATTCTTTTAGGTTGCCTATTATTTCATCTTTCTTTTGTGTTAGTAGTTTGACCGTTTTTATCGTTGCTTCTTTGTTTATGCTTCTTGATGTGGTTATTACCGCGCTGCGGTTTTGAACTCATATAGCTTACACACTTTTTAGTTTGTTTTATTTCTCCGTTTATTTCTTGTGCTTTTTGTGTTATTTTTATAGTAATCAATTCTTCTGGGGGTTCTTTTCCGTTCTGTGTTTCACTAACCTTAATCGTTAAAACATTCCCCTTTTCCTGAGAAT"..b"TCCTGTTTTCGGGGTAAAACATCTCTTT\n++\n+(?A@=CE-3()(49))6,69>H>::/(79,%3(0:&&%*3)-0/23-5$)**+&6;<),%:CC=5?7562**'%%61>=?:;FDF@?:4>56>?3/(<;)0*02/36<<;AAABCDFE::JIF<9;5:4<:?>@-)'3'.1$&&$&,;:00C?'4=>((,@D9H?<B<>@B<;=HG=<L(=()(&7<>@=F<;CB>D?=\n+@7a97d860-e33a-4e27-b5be-c4754a2eb907\n+ATTACGAAGTATCACGTGCCCTGTTATCCAGACTTATGGCAGATTAGCTTCCCGGAGAGAAAACTGTCGAAAACAGACGGTATGAACACCGTAAGCTCCAAAGTGATCACCATTCGCTTTCATGCGCCTTGGCTATGCAGCGAGCTGAAAACGATCCTGACGCATCCTTCCTGTTTTCCCGAAGGGTAAAACATCTCTTT\n++\n+?>A?'-$%+?'%8$03F;;;=8*57>((?3:E?E?65%,0,.,B>77?AA6<G<9C(,):0IGAD=;AEIJLC@'?3A+/68966>9?<>??:</:<-.'(%32228@;:=CGDA>78:@97424%%$'%')460((8,:;1/4////@;5;?@//5896:.+?:<ACA>@KEA@LD=C22.+01849?D<>@;A=<K5/\n+@cc6e152f-3be2-4154-95c1-265c4f77a7ac\n+TACGGAGTATCACCGTGCCTGTTATCCAGACTTATGGCAGATTAGCTTCCCGGAGAAAACTGCTCCAACAGACGGTATGAACACCGTAAGCTCCCAGAAGTGATCACCATTCGCTTTCTCATGCATAGCTATGCGGCGAGCTGAAAAGCTGATCCTGACGCATCCTTCCTGTTTTTCCCGGGAGTAAAACATCTCTTT\n++\n+,*635,44()6,87.6'66=(@;0**E=DFCL?77-56732,/(-./1-.//17&(5EE;:)')(6,,4=8>C29;5>8832,>>459;+;;)-(+$%$*77;6CAABDB?-0*+,(&$+&**8:85=??@))?%.7+*4/+(*.'($#%,,++4;EBB:B>78=8<=@GBDCGB@5;A+1<$6:EGDAG;:>>@@?H\n+@749ba682-4ec2-4367-8bb3-51878f06992d\n+CCGGGAAAGCGGAAGGATCGTCAGGATCGTTGCTACAGCTCTGCTACATATGCATGAAAGCGAATGGTGATGCTTTGGGACTTTACGGTGTTTCATACCGTCTGTTTTCGACAGTTTTCTCTCCAGGGAAACTAATCTGCCAGCAAGTCTGGATAACAGGG\n++\n+#$$(+('&%,$)5;:8/&(,121165++.-/$$(%)(%#%'#$$$$&&%$%(3/1<222(666:=8<47/&$&)),&'&',-/.39=B>B?=-89;=>=;;;335686E;=9499=PA@-8<%/'/*+,)$77=7/-5//.-&(()**7',./102,&,&'\n+@3d6bdcab-ef5c-4ff8-a928-23066fb4e887\n+CTGTTATCAGACTTATGGCAGATTAGCTTCCCGGAGAAGAAAACTGTCGAAAACAGACGGTATGAACACCGTAAGCTCCCAAAAGTGATCACCATTCATCATGCATAGCTATGCGGGCAGACTGCGAAAACGATCCTGACTCCTTCCTGTTTTCCGGGGTAAAACATCTCTTT\n++\n+12551)$>,,/,,,AE??>==?6/.'''-8>B03<+,,-=CA@<A@<A98=<17581:*0<BC66@AA<>;;/09:6470999-%171+98==8<;6#'36;AC;7:689<<89%*./(&$++$-+--<-;=>55:4;*%&%*%/454@CAHA/318?G?4599=9?DEA@?E\n+@0b41ea60-ea37-43ef-a912-260c4d33a356\n+CTGTTATCCAGACTTATGGCAGATTAGCTTCCGGAGAAAACTGCTTCGAAAAACAGACGGTATGAACACCGTAAGCTCCCAAAGTGATCACCATTCGCTTTCATGCATAGCGCTATGCAGCGAGCTGAAAACGATCTGACGCATCCTTCCTGTTTTCCCGGGGTAAAACATCTCTTT\n++\n+=B>B3+++7-2-))%&.%,3**749689:=1B0.3&)(-'$$%#%(*(((('$,)+(/+,,/48:>;62+7:)*.<;9>@DE75@?<?GFGD@DDA$$=>;>?::>-043+).2?;<(&551.;><:8G@6E@<6@7<6@1CE78BDB>?=?57I@'-G3;@B=>@F@?8<?>794-\n+@cd8d2944-a807-434b-92d5-bfa925697c85\n+GTTATCCAGACTTATGGCAGATTAGCTTCCCGGAGAGAAAACTGGCATGAAAACAGACAGGTATGAACACCGTAAACTCGGTGATCACCATTCGCTTTCATGCATAGCTATGCAGCGAGCTGAAAACGATCCTGACGCATCTCCTTCCTGTTTTCCGGGGTAAAACATCTCTTT\n++\n+8?>>%%06/B@FEFG7=854742')9<=+3B9:<98%;<>3..-(&$*/13,*;8;1+&59ADB55<94<<:;99(&)+#&62-'('668:7855;=C><24B=>==>;B<:30=;=1;9575IF9A=E55<:@?GC;77=069<=25152/56)/,45?>@BE162;>:9DCE\n+@a492dd23-2c03-4d9b-a606-dee7268bd4fa\n+AAAGAGATGTTTTTGCGGAAAACAGGAAGGATGCGTCAGGATCGTTTTCAGCGCTATAGCTATGCATGAAAGCGAATGGTGATCCTTTGGGAGCTTACGGTGTTCATGCCAATCTGTTTTCGACAGTTTCTCTCCGAGGAAGCTAATCTGCCGCAAGTCTAGTTAAC\n++\n+F:&-/77;:@??=8%%$(#'+()%+9.99AB?==<<7/;;4/;;B>?-+(*2%%&0-1-=5;99GD=88>9>??=IC2:B?:%%*#*-&.1**,--;A::8BID?4&&%8'(<AE3??;C@>B64>:;31&*,(5('7A04.>@CC;AA7%1-1%&-/5'%*%-+,/\n+@4a58265a-1d25-47a1-b851-e7687203734f\n+GGGAAACCCAGGAGGATCGTCAGGATCGTTTTTTCAGCTCGCTGCTATGTATGCGCTGAAAGCGAATGGTGATCACTTTGGGGAGCTTACGGTGTTCATACTGTCTGTTTTCGACAGTTTTCTCTCCGGGAAGCTAATCTGCCATAAGTCTGGATAAC\n++\n+)**%&&%5'%)*$..5%$$,70?B?:>?GBLB<0))%&'-78>.11/$$+($&$&%***2),=88=@,1A=:3<25;<959E;.9?BB*A<CCHBBB==BCC>=B>B9?LL;AA74C>CNEDF<C%?7<D78.=>C=4=?@BB9@>>2-A14:C;..5\n+@f9a56724-1fa4-4807-bacc-f0a85587afd7\n+GAACACCGTAAGCTCCCAAAGTGATCACCATTCGCTTTTTCATGCATAGCTATGCAGCGACTGGCGATCCTGACGCATCTTCTGTTTTCC\n++\n+414===BA;3-.21$+&')'%-20-,((+6:57(*+,139@@?A534546/24022**)$$$#&(--++-7<+6*.*(())(,-03<:(,\n+@e525f31a-e4d6-4fe6-a703-7038efa2f178\n+GCTATGCAGCGAGCTGAAAACGATCCTGACGCATCCTTCCTGTTTCCCGGGTAAAACATCTCTTT\n++\n+;B@E::0+5511=2122@>52((''*<G=E:?A45:79$=JFCC?AJG*024<>HECB@C@?KHI\n+@eeef3ccf-2188-4382-8399-a9442bbbf40a\n+GCTATGCAGCGAGCTGAAAACGATCCTGACGCATCCTTCCTGTTTTCCCGGGGTAAAACATCTCTTT\n++\n+@=:3335937))43378KC?<?A<>CKMEH?BC55=4C>?KDFCLC4@G363877800+7<=;3;;>\n" |