| Next changeset 1:642849141ae9 (2014-04-10) |
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Commit message:
Imported from capsule None |
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added:
basecoverage.xml gops_basecoverage.py operation_filter.py test-data/1.bed test-data/gops_basecoverage_out.txt test-data/gops_basecoverage_out2.txt test-data/gops_bigint.interval tool_dependencies.xml |
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| diff -r 000000000000 -r 1e6a9e97fa41 basecoverage.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/basecoverage.xml Tue Apr 01 10:53:19 2014 -0400 |
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| @@ -0,0 +1,49 @@ +<tool id="gops_basecoverage_1" name="Base Coverage" version="0.0.1"> + <description>of all intervals</description> + <command interpreter="python">gops_basecoverage.py $input1 $output -1 ${input1.metadata.chromCol},${input1.metadata.startCol},${input1.metadata.endCol},${input1.metadata.strandCol}</command> + <requirements> + <requirement type="package" version="0.7.1">bx-python</requirement> + <requirement type="package" version="1.0.0">galaxy-ops</requirement> + </requirements> + <inputs> + <param format="interval" name="input1" type="data"> + <label>Compute coverage for</label> + </param> + </inputs> + <outputs> + <data format="txt" name="output" /> + </outputs> + <code file="operation_filter.py"/> + <tests> + <test> + <param name="input1" value="1.bed" /> + <output name="output" file="gops_basecoverage_out.txt" /> + </test> + <test> + <param name="input1" value="gops_bigint.interval" /> + <output name="output" file="gops_basecoverage_out2.txt" /> + </test> + </tests> + <help> + +.. class:: infomark + +**TIP:** If your dataset does not appear in the pulldown menu, it means that it is not in interval format. Use "edit attributes" to set chromosome, start, end, and strand columns. + +This operation counts the total bases covered by a set of intervals. Bases that are covered by more than one interval are **not** counted more than once towards the total. + +----- + +**Screencasts!** + +See Galaxy Interval Operation Screencasts_ (right click to open this link in another window). + +.. _Screencasts: http://wiki.g2.bx.psu.edu/Learn/Interval%20Operations + +**Example** + +.. image:: ${static_path}/operation_icons/gops_baseCoverage.gif + + +</help> +</tool> |
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| diff -r 000000000000 -r 1e6a9e97fa41 gops_basecoverage.py --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/gops_basecoverage.py Tue Apr 01 10:53:19 2014 -0400 |
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| @@ -0,0 +1,48 @@ +#!/usr/bin/env python +""" +Count total base coverage. + +usage: %prog in_file out_file + -1, --cols1=N,N,N,N: Columns for start, end, strand in first file +""" + +import sys, traceback, fileinput +from warnings import warn +from bx.intervals import * +from bx.intervals.io import * +from bx.intervals.operations.base_coverage import * +from bx.cookbook import doc_optparse +from galaxy.tools.util.galaxyops import * + +assert sys.version_info[:2] >= ( 2, 4 ) + +def main(): + upstream_pad = 0 + downstream_pad = 0 + + options, args = doc_optparse.parse( __doc__ ) + try: + chr_col_1, start_col_1, end_col_1, strand_col_1 = parse_cols_arg( options.cols1 ) + in_fname, out_fname = args + except: + doc_optparse.exception() + + g1 = NiceReaderWrapper( fileinput.FileInput( in_fname ), + chrom_col=chr_col_1, + start_col=start_col_1, + end_col=end_col_1, + strand_col = strand_col_1, + fix_strand=True ) + + try: + bases = base_coverage(g1) + except ParseError, exc: + fail( "Invalid file format: %s" % str( exc ) ) + out_file = open( out_fname, "w" ) + out_file.write( "%s\n" % str( bases ) ) + out_file.close() + if g1.skipped > 0: + print skipped( g1, filedesc="" ) + +if __name__ == "__main__": + main() |
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| diff -r 000000000000 -r 1e6a9e97fa41 operation_filter.py --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/operation_filter.py Tue Apr 01 10:53:19 2014 -0400 |
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| @@ -0,0 +1,99 @@ +# runs after the job (and after the default post-filter) +import os +from galaxy import eggs +from galaxy import jobs +from galaxy.tools.parameters import DataToolParameter + +from galaxy.jobs.handler import JOB_ERROR + +# Older py compatibility +try: + set() +except: + from sets import Set as set + +#def exec_before_process(app, inp_data, out_data, param_dict, tool=None): +# """Sets the name of the data""" +# dbkeys = sets.Set( [data.dbkey for data in inp_data.values() ] ) +# if len(dbkeys) != 1: +# raise Exception, '<p><font color="yellow">Both Queries must be from the same genome build</font></p>' + +def validate_input( trans, error_map, param_values, page_param_map ): + dbkeys = set() + data_param_names = set() + data_params = 0 + for name, param in page_param_map.iteritems(): + if isinstance( param, DataToolParameter ): + # for each dataset parameter + if param_values.get(name, None) != None: + dbkeys.add( param_values[name].dbkey ) + data_params += 1 + # check meta data + try: + param = param_values[name] + if isinstance( param.datatype, trans.app.datatypes_registry.get_datatype_by_extension( 'gff' ).__class__ ): + # TODO: currently cannot validate GFF inputs b/c they are not derived from interval. + pass + else: # Validate interval datatype. + startCol = int( param.metadata.startCol ) + endCol = int( param.metadata.endCol ) + chromCol = int( param.metadata.chromCol ) + if param.metadata.strandCol is not None: + strandCol = int ( param.metadata.strandCol ) + else: + strandCol = 0 + except: + error_msg = "The attributes of this dataset are not properly set. " + \ + "Click the pencil icon in the history item to set the chrom, start, end and strand columns." + error_map[name] = error_msg + data_param_names.add( name ) + if len( dbkeys ) > 1: + for name in data_param_names: + error_map[name] = "All datasets must belong to same genomic build, " \ + "this dataset is linked to build '%s'" % param_values[name].dbkey + if data_params != len(data_param_names): + for name in data_param_names: + error_map[name] = "A dataset of the appropriate type is required" + +# Commented out by INS, 5/30/2007. What is the PURPOSE of this? +def exec_after_process(app, inp_data, out_data, param_dict, tool=None, stdout=None, stderr=None): + """Verify the output data after each run""" + items = out_data.items() + + for name, data in items: + try: + if stderr and len( stderr ) > 0: + raise Exception( stderr ) + + except Exception, exc: + data.blurb = JOB_ERROR + data.state = JOB_ERROR + +## def exec_after_process(app, inp_data, out_data, param_dict, tool=None, stdout=None, stderr=None): +## pass + + +def exec_after_merge(app, inp_data, out_data, param_dict, tool=None, stdout=None, stderr=None): + exec_after_process( + app, inp_data, out_data, param_dict, tool=tool, stdout=stdout, stderr=stderr) + + # strip strand column if clusters were merged + items = out_data.items() + for name, data in items: + if param_dict['returntype'] == True: + data.metadata.chromCol = 1 + data.metadata.startCol = 2 + data.metadata.endCol = 3 + # merge always clobbers strand + data.metadata.strandCol = None + + +def exec_after_cluster(app, inp_data, out_data, param_dict, tool=None, stdout=None, stderr=None): + exec_after_process( + app, inp_data, out_data, param_dict, tool=tool, stdout=stdout, stderr=stderr) + + # strip strand column if clusters were merged + if param_dict["returntype"] == '1': + items = out_data.items() + for name, data in items: + data.metadata.strandCol = None |
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| diff -r 000000000000 -r 1e6a9e97fa41 test-data/1.bed --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/1.bed Tue Apr 01 10:53:19 2014 -0400 |
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| @@ -0,0 +1,65 @@ +chr1 147962192 147962580 CCDS989.1_cds_0_0_chr1_147962193_r 0 - +chr1 147984545 147984630 CCDS990.1_cds_0_0_chr1_147984546_f 0 + +chr1 148078400 148078582 CCDS993.1_cds_0_0_chr1_148078401_r 0 - +chr1 148185136 148185276 CCDS996.1_cds_0_0_chr1_148185137_f 0 + +chr10 55251623 55253124 CCDS7248.1_cds_0_0_chr10_55251624_r 0 - +chr11 116124407 116124501 CCDS8374.1_cds_0_0_chr11_116124408_r 0 - +chr11 116206508 116206563 CCDS8377.1_cds_0_0_chr11_116206509_f 0 + +chr11 116211733 116212337 CCDS8378.1_cds_0_0_chr11_116211734_r 0 - +chr11 1812377 1812407 CCDS7726.1_cds_0_0_chr11_1812378_f 0 + +chr12 38440094 38440321 CCDS8736.1_cds_0_0_chr12_38440095_r 0 - +chr13 112381694 112381953 CCDS9526.1_cds_0_0_chr13_112381695_f 0 + +chr14 98710240 98712285 CCDS9949.1_cds_0_0_chr14_98710241_r 0 - +chr15 41486872 41487060 CCDS10096.1_cds_0_0_chr15_41486873_r 0 - +chr15 41673708 41673857 CCDS10097.1_cds_0_0_chr15_41673709_f 0 + +chr15 41679161 41679250 CCDS10098.1_cds_0_0_chr15_41679162_r 0 - +chr15 41826029 41826196 CCDS10101.1_cds_0_0_chr15_41826030_f 0 + +chr16 142908 143003 CCDS10397.1_cds_0_0_chr16_142909_f 0 + +chr16 179963 180135 CCDS10401.1_cds_0_0_chr16_179964_r 0 - +chr16 244413 244681 CCDS10402.1_cds_0_0_chr16_244414_f 0 + +chr16 259268 259383 CCDS10403.1_cds_0_0_chr16_259269_r 0 - +chr18 23786114 23786321 CCDS11891.1_cds_0_0_chr18_23786115_r 0 - +chr18 59406881 59407046 CCDS11985.1_cds_0_0_chr18_59406882_f 0 + +chr18 59455932 59456337 CCDS11986.1_cds_0_0_chr18_59455933_r 0 - +chr18 59600586 59600754 CCDS11988.1_cds_0_0_chr18_59600587_f 0 + +chr19 59068595 59069564 CCDS12866.1_cds_0_0_chr19_59068596_f 0 + +chr19 59236026 59236146 CCDS12872.1_cds_0_0_chr19_59236027_r 0 - +chr19 59297998 59298008 CCDS12877.1_cds_0_0_chr19_59297999_f 0 + +chr19 59302168 59302288 CCDS12878.1_cds_0_0_chr19_59302169_r 0 - +chr2 118288583 118288668 CCDS2120.1_cds_0_0_chr2_118288584_f 0 + +chr2 118394148 118394202 CCDS2121.1_cds_0_0_chr2_118394149_r 0 - +chr2 220190202 220190242 CCDS2441.1_cds_0_0_chr2_220190203_f 0 + +chr2 220229609 220230869 CCDS2443.1_cds_0_0_chr2_220229610_r 0 - +chr20 33330413 33330423 CCDS13249.1_cds_0_0_chr20_33330414_r 0 - +chr20 33513606 33513792 CCDS13255.1_cds_0_0_chr20_33513607_f 0 + +chr20 33579500 33579527 CCDS13256.1_cds_0_0_chr20_33579501_r 0 - +chr20 33593260 33593348 CCDS13257.1_cds_0_0_chr20_33593261_f 0 + +chr21 32707032 32707192 CCDS13614.1_cds_0_0_chr21_32707033_f 0 + +chr21 32869641 32870022 CCDS13615.1_cds_0_0_chr21_32869642_r 0 - +chr21 33321040 33322012 CCDS13620.1_cds_0_0_chr21_33321041_f 0 + +chr21 33744994 33745040 CCDS13625.1_cds_0_0_chr21_33744995_r 0 - +chr22 30120223 30120265 CCDS13897.1_cds_0_0_chr22_30120224_f 0 + +chr22 30160419 30160661 CCDS13898.1_cds_0_0_chr22_30160420_r 0 - +chr22 30665273 30665360 CCDS13901.1_cds_0_0_chr22_30665274_f 0 + +chr22 30939054 30939266 CCDS13903.1_cds_0_0_chr22_30939055_r 0 - +chr5 131424298 131424460 CCDS4149.1_cds_0_0_chr5_131424299_f 0 + +chr5 131556601 131556672 CCDS4151.1_cds_0_0_chr5_131556602_r 0 - +chr5 131621326 131621419 CCDS4152.1_cds_0_0_chr5_131621327_f 0 + +chr5 131847541 131847666 CCDS4155.1_cds_0_0_chr5_131847542_r 0 - +chr6 108299600 108299744 CCDS5061.1_cds_0_0_chr6_108299601_r 0 - +chr6 108594662 108594687 CCDS5063.1_cds_0_0_chr6_108594663_f 0 + +chr6 108640045 108640151 CCDS5064.1_cds_0_0_chr6_108640046_r 0 - +chr6 108722976 108723115 CCDS5067.1_cds_0_0_chr6_108722977_f 0 + +chr7 113660517 113660685 CCDS5760.1_cds_0_0_chr7_113660518_f 0 + +chr7 116512159 116512389 CCDS5771.1_cds_0_0_chr7_116512160_r 0 - +chr7 116714099 116714152 CCDS5773.1_cds_0_0_chr7_116714100_f 0 + +chr7 116945541 116945787 CCDS5774.1_cds_0_0_chr7_116945542_r 0 - +chr8 118881131 118881317 CCDS6324.1_cds_0_0_chr8_118881132_r 0 - +chr9 128764156 128764189 CCDS6914.1_cds_0_0_chr9_128764157_f 0 + +chr9 128787519 128789136 CCDS6915.1_cds_0_0_chr9_128787520_r 0 - +chr9 128882427 128882523 CCDS6917.1_cds_0_0_chr9_128882428_f 0 + +chr9 128937229 128937445 CCDS6919.1_cds_0_0_chr9_128937230_r 0 - +chrX 122745047 122745924 CCDS14606.1_cds_0_0_chrX_122745048_f 0 + +chrX 152648964 152649196 CCDS14733.1_cds_0_0_chrX_152648965_r 0 - +chrX 152691446 152691471 CCDS14735.1_cds_0_0_chrX_152691447_f 0 + +chrX 152694029 152694263 CCDS14736.1_cds_0_0_chrX_152694030_r 0 - |
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| diff -r 000000000000 -r 1e6a9e97fa41 test-data/gops_basecoverage_out.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/gops_basecoverage_out.txt Tue Apr 01 10:53:19 2014 -0400 |
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| @@ -0,0 +1,1 @@ +17987 |
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| diff -r 000000000000 -r 1e6a9e97fa41 test-data/gops_basecoverage_out2.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/gops_basecoverage_out2.txt Tue Apr 01 10:53:19 2014 -0400 |
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| @@ -0,0 +1,1 @@ +12117 |
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| diff -r 000000000000 -r 1e6a9e97fa41 test-data/gops_bigint.interval --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/gops_bigint.interval Tue Apr 01 10:53:19 2014 -0400 |
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| @@ -0,0 +1,9 @@ +chrM 69 852 uc009vev.1_exon_0_0_chrM_70_f 0 + +chrM 1148 3703 uc009vew.1_exon_0_0_chrM_1149_f 0 + +chrM 3848 4933 uc009vex.1_exon_0_0_chrM_3849_f 0 + +chrM 5326 6938 uc009vey.1_exon_0_0_chrM_5327_f 0 + +chrM 7009 7699 uc009vez.1_exon_0_0_chrM_7010_f 0 + +chrM 7765 8607 uc009vfa.1_exon_0_0_chrM_7766_f 0 + +chrM 9875 11542 uc009vfb.1_exon_0_0_chrM_9876_f 0 + +chrM 12405 15288 uc009vfc.1_exon_0_0_chrM_12406_f 0 + +chrM 4294967295 4294967322 EAS38_1_45_638_677 2 + \ No newline at end of file |
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| diff -r 000000000000 -r 1e6a9e97fa41 tool_dependencies.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool_dependencies.xml Tue Apr 01 10:53:19 2014 -0400 |
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| @@ -0,0 +1,9 @@ +<?xml version="1.0"?> +<tool_dependency> + <package name="bx-python" version="0.7.1"> + <repository changeset_revision="41eb9d9f667d" name="package_bx_python_0_7" owner="devteam" prior_installation_required="False" toolshed="http://toolshed.g2.bx.psu.edu" /> + </package> + <package name="galaxy-ops" version="1.0.0"> + <repository changeset_revision="4e39032e4ec6" name="package_galaxy_ops_1_0_0" owner="devteam" prior_installation_required="False" toolshed="http://toolshed.g2.bx.psu.edu" /> + </package> +</tool_dependency> |