Repository 'prinseq'
hg clone https://toolshed.g2.bx.psu.edu/repos/iuc/prinseq

Changeset 5:1ee282794de3 (2022-03-20)
Previous changeset 4:654b3a274ed5 (2021-07-07)
Commit message:
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/prinseq/ commit 34e8262534e22f0d391a81b06374744c4af8da24"
modified:
prinseq.xml
added:
test-data/pairend_strandspecific_51mer_hg19_chr1_1-100000.R1.fastq
test-data/pairend_strandspecific_51mer_hg19_chr1_1-100000.R1.fastq.gz
test-data/pairend_strandspecific_51mer_hg19_chr1_1-100000.R2.fastq
test-data/pairend_strandspecific_51mer_hg19_chr1_1-100000.R2.fastq.gz
test-data/prinseq_input_sequences.fastq.gz
removed:
test-data/prinseq_good_sequences.fastq
test-data/prinseq_input_sequences.fastq
b
diff -r 654b3a274ed5 -r 1ee282794de3 prinseq.xml
--- a/prinseq.xml Wed Jul 07 09:22:27 2021 +0000
+++ b/prinseq.xml Sun Mar 20 10:51:09 2022 +0000
[
b'@@ -1,5 +1,8 @@\n-<tool id="prinseq" name="PRINSEQ" version="@TOOL_VERSION+galaxy1">\n+<tool id="prinseq" name="PRINSEQ" version="@TOOL_VERSION+galaxy2" profile="20.05">\n     <description>to process quality of sequences</description>\n+    <xrefs>\n+        <xref type="bio.tools">prinseq</xref>\n+    </xrefs>\n     <macros>\n         <token name="@TOOL_VERSION">0.20.4</token>\n     </macros>\n@@ -8,7 +11,6 @@\n     </requirements>\n \n     <stdio>\n-        <exit_code range="1:"   level="fatal"   description="" />\n         <regex match="ERROR"\n                source="stderr"\n                level="fatal"\n@@ -25,36 +27,57 @@\n ]]>\n     </version_command>\n \n-    <command>\n+    <command detect_errors="exit_code">\n <![CDATA[\n-        mkdir tmp/\n+        mkdir tmp/ &&\n+\n+        #if $seq_type.seq_type_opt == "single"\n+            #set fwd = $seq_type.input_singles\n+            #set rev = None\n+        #else if $seq_type.seq_type_opt == "paired"\n+            #set fwd = $seq_type.input_mate1\n+            #set rev = $seq_type.input_mate2\n+        #else\n+            #set fwd = $seq_type.input_collection.forward\n+            #set rev = $seq_type.input_collection.reverse\n+        #end if\n \n-        &&\n+        #if $rev and $fwd.ext != $rev.ext:\n+            >&2 echo \'Both pairs from your paired-end library need to be from the same filetype.\' &&\n+            exit 1;\n+        #end if\n+        \n+        #if $fwd.ext.endswith(".gz")\n+            gunzip -c \'$fwd\' > fwd.fastq &&\n+        #else\n+            ln -s \'$fwd\' fwd.fastq &&\n+        #end if\n+        \n+        #if $rev\n+            #if $rev.ext.endswith(".gz")\n+                gunzip -c \'$rev\' > rev.fastq &&\n+            #else\n+                ln -s \'$rev\' rev.fastq &&\n+            #end if\n+        #end if\n+\n+        ## create empty output files\n+        #if $seq_type.seq_type_opt == "single"\n+            touch tmp/good_sequences.fastq tmp/rejected_sequences.fastq &&\n+        #else\n+            touch tmp/good_sequences_1.fastq tmp/good_sequences_1_singletons.fastq tmp/rejected_sequences_1.fastq &&\n+            touch tmp/good_sequences_2.fastq tmp/good_sequences_2_singletons.fastq tmp/rejected_sequences_2.fastq &&\n+        #end if\n+\n \n         prinseq-lite.pl\n-            #if $seq_type.seq_type_opt == "single":\n-                -fastq \'$seq_type.input_singles\'\n-                #if $seq_type.input_singles.is_of_type(\'fastqillumina\'):\n-                    -phred64\n-                #end if\n-            #elif $seq_type.seq_type_opt == "paired":\n-                -fastq \'$seq_type.input_mate1\'\n-                -fastq2 \'$seq_type.input_mate2\'\n-                #if $seq_type.input_mate1.ext != $seq_type.input_mate2.ext:\n-                    #import sys\n-                    #silent sys.stderr.write( \'Both pairs from your paired-end library need to be from the same filetype.\' )\n-                #end if\n-                #if $seq_type.input_mate1.is_of_type(\'fastqillumina\'):\n-                    -phred64\n-                #end if\n-            #else \n-                -fastq \'$seq_type.input_collection.forward\'\n-                -fastq2 \'$seq_type.input_collection.reverse\'\n-                #if $seq_type.input_collection.forward.is_of_type(\'fastqillumina\'):\n-                    -phred64\n-                #end if\n+            -fastq fwd.fastq\n+            #if $rev\n+                -fastq2 rev.fastq\n             #end if\n-\n+            #if $fwd.ext.startswith(\'fastqillumina\'):\n+                -phred64\n+            #end if\n             -out_good "tmp/good_sequences"\n             -out_bad "tmp/rejected_sequences"\n \n@@ -226,9 +249,16 @@\n \n         prinseq-graphs-noPCA.pl -i "tmp/stats.gd" -html_all -o stats_html\n         *#\n+\n+        #if $fwd.ext.endswith(\'.gz\')\n+            && for f in tmp/*.fastq;\n+            do\n+                gzip -c \\$f > tmp_file &&\n+                mv tmp_file \\$f;\n+            done\n+        #end if\n ]]>\n     </command>\n-\n     <inputs>\n         <conditional name="seq_type">\n             <param nam'..b'treatments\' value="true"/>\n+            <param name="min_length_filter_treatment_value" value="50"/>\n+            <param name=\'apply_max_length_filter_treatments\' value="false" />\n+            <param name=\'apply_quality_filter_treatments\' value="true"/>\n+            <param name=\'apply_min_quality_filter_treatments\' value="false" />\n+            <param name=\'apply_max_quality_filter_treatments\' value="false"/>\n+            <param name=\'apply_mean_quality_filter_treatments\' value="true"/>\n+            <param name=\'apply_min_mean_quality_filter_treatments\' value="true"/>\n+            <param name="min_mean_quality_filter_treatment_value" value="15"/>\n+            <param name=\'apply_max_mean_quality_filter_treatments\' value="false"/>\n+            <param name=\'apply_base_content_filter_treatments\' value="true"/>\n+            <param name=\'apply_GC_perc_content_filter_treatments\' value="false"/>\n+            <param name=\'apply_N_number_content_filter_treatments\' value="false"/>\n+            <param name=\'apply_N_percentage_content_filter_treatments\' value="true"/>\n+            <param name="N_percentage_content_filter_treatment_value" value="2"/>\n+            <param name=\'apply_other_base_content_filter_treatments\' value="false"/>\n+            <param name=\'apply_complexity_filter_treatments\' value="false"/>\n+            <param name=\'apply_trimming_treatments\' value="true" />\n+            <param name=\'apply_length_trimming_treatments\' value="false"/>\n+            <param name=\'apply_position_trimming_treatments\' value="false"/>\n+            <param name=\'apply_tail_trimming_treatments\' value="false"/>\n+            <param name=\'apply_quality_trimming_treatments\' value="true"/>\n+            <param name=\'apply_left_quality_trimming_treatments\' value="false"/>\n+            <param name=\'apply_right_quality_trimming_treatments\' value="true" />\n+            <param name="right_quality_trimming_treatment_value" value="20"/>\n+            <param name="type_quality_trimming_treatments" value="min"/>\n+            <param name="rule_quality_trimming_treatments" value="lt" />\n+            <param name="window_quality_trimming_treatments" value="1"/>\n+            <param name="step_quality_trimming_treatments" value="1"/>\n+            <output_collection name="good_sequences_collection" type="paired">\n+                <element name="forward" ftype="fastqsanger.gz">\n+                    <assert_contents>\n+                        <has_size value="605" delta="100"/>\n+                    </assert_contents>\n+                </element>\n+                <element name="reverse" ftype="fastqsanger.gz">\n+                    <assert_contents>\n+                        <has_size value="667" delta="100"/>\n+                    </assert_contents>\n+                </element>\n+            </output_collection>\n+            <output_collection name="singletons_collection" type="paired">\n+                <element name="forward" ftype="fastqsanger.gz">\n+                    <assert_contents>\n+                        <has_size value="720" delta="100"/>\n+                    </assert_contents>\n+                </element>\n+                <element name="reverse" ftype="fastqsanger.gz">\n+                    <assert_contents>\n+                        <has_size value="219" delta="100"/>\n+                    </assert_contents>\n+                </element>\n+            </output_collection>\n+            <output_collection name="rejected_sequences_collection" type="paired">\n+                <element name="forward" ftype="fastqsanger.gz">\n+                    <assert_contents>\n+                        <has_size value="0" delta="0"/>\n+                    </assert_contents>\n+                </element>\n+                <element name="reverse" ftype="fastqsanger.gz">\n+                    <assert_contents>\n+                        <has_size value="718" delta="100"/>\n+                    </assert_contents>\n+                </element>\n+            </output_collection>\n         </test>\n     </tests>\n \n'
b
diff -r 654b3a274ed5 -r 1ee282794de3 test-data/pairend_strandspecific_51mer_hg19_chr1_1-100000.R1.fastq
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/pairend_strandspecific_51mer_hg19_chr1_1-100000.R1.fastq Sun Mar 20 10:51:09 2022 +0000
[
@@ -0,0 +1,80 @@
+@seq.11990047/1
+ACGGCCGACTTGGATCACACTCTTGCAGGGCCATCAGGCACCAAAGGGATT
++
+hgffghhhhfhhchfhghhhhhhhggahh[chffhfhghhhhhhafehfeh
+@seq.14614493/1
+AGAGGAGGACGAGGACGACTGGGAATCGTAGGGGGCTCCATGACACCTTCC
++
+hhgghehehghgghhaffffcggchghhhhgfhfhahghhhghhghhhhhe
+@seq.24018133/1
+CGGGTGGATTTTCTGTGGGTTTGTTAAGTGGTCAGAAATTCTCAATTTTTT
++
+`aggggecfffa\\^Ua``\af`fffcffffafaffcffffec``fWfffe
+@seq.10608403/1
+GTATGGCCAGAGGGCAGGGCCGAGGGGTGTGGGCGGGAGGCCCGGCCTGGC
++
+_dd_]bfggfgcg[egdbdbdXc`cfggaagdgggf^ggfdfggggggggg
+@seq.10820209/1
+GTGCTGGCCCCAGTTTTCTAACCAGGTGTTGAATGAACTGGATGGACTCTG
++
+ghhccgfgghhhdhfhhghhhfffdf_hfhhhffhhhgdchhhhhgfhahh
+@seq.1537155/1
+GGGAGTGTGCAGAGACTGGAGGGGATGACAGTCACCCTCTGTTTTCTGTGG
++
+aag`hhhhhgghhhhhhfhhchgfacchhhhah]hhdcafhhhhffachhg
+@seq.25274725/1
+AGGGTGTGGGGCAAGGCAGTGAGTGAAGAGTTGGGATGAGTGAGTTAGGGC
++
+hhhhdhhhhhhffffcfdffff_fdffffffhchahhhhchgfhhfhfhhh
+@seq.26326595/1
+GGGAAGGGGGTGCTTCTGCATGGGAAGCACAGACAGCGCTGCCTCTCCCTT
++
+Wbfdf]ddggggdgggdfdfWggdggf]fffadfffffVfffgfgfdgggg
+@seq.28833653/1
+TGGGGCCAGGGGACTATGACACACCACTTGGCTTAGACTGAGGAGCTCTGT
++
+_cffafhdghhhhhhhhhhhghhaffhhhhdhfhhehhhhhhgghfhghhh
+@seq.25049090/1
+AGGGCGAGATTGATTGTTAATTGCTAGCATGAACCGCGTGGGCTTCTCAGG
++
+fdffbfdddb[_afffacdggafdbc[fcfcgggfgffccfgagggggfgc
+@seq.23476912/1
+GGCCTCTCCACCATGTGCTCCACCTCGTGCTGGACCTTAAGAGATACCAAT
++
+fgggggeggecfefffd^^aY]fdfcaggggfdefdggggggggggggggg
+@seq.28059536/1
+GGGATGAGGAGAGGGCAGGAAGGCATTTCCTGGGTAGTGGAGTGCTGTGTT
++
+B_bbea[_V[WZVY`\Pacaaebecd]]fddbaed[decbe]fd`fggggg
+@seq.13270875/1
+TGCCCCGAGTTTGTCAAGAATGTCCCAGTAACCAGGGGACACACAGTGAAG
++
+ffffafgagcggggfgfcfffccfcffg]ggggfgcgggggggggggggcf
+@seq.2214586/1
+GTGGGAGGGGCTGAAGTGAGAGCCCAACTTGGAAGCTTTTACTCCTGGGAG
++
+gghghghhhhhhhhhhffefafhfhhhhhgghhhghhehhhhehhhhhhhh
+@seq.31061198/1
+GAGGAGCTAGGGTTTCTCATAAAACTCCCTGATAGAAGACGACTTTTGATA
++
+cd\WaaaRcaacJdd[dff_f_ffcfddfff_dafffcd[cd\aW\eedcc
+@seq.13835843/1
+GGGGAGGCAGAGGTTGCAGTGAGCCGAGATCATGTCACTGCACTCCAGGCT
++
+ggcgafggfgggggggfgggagggagggefgdffffdadeggggggggegg
+@seq.13539256/1
+GTGGGGAAACCTAGAATTGCTGTAGAGAAAATGCCCTAGAGCAGCTCTAGA
++
+hfhhchfhhhfhghhhhhhhhghghhhgfhhhhhhhhhghhhhfhhhhfhh
+@seq.5556605/1
+GGGATGAGGCCAAATCTTTCTGAATCTGAGATAGCCTCTCAGCCTATGCAT
++
+hfhchhchhhehhhhhhhhdhh_hghchgfhdhhhhhhhhhchhghhhhhh
+@seq.32597077/1
+GAGAGACGGGGTTTCACCATGTTGGCCAGGATGGTCTTGATCTCTTGACCT
++
+dcba]fffcdfdWccf\``S_da_cdc_fdafggggfffcfcfcfddafff
+@seq.20367385/1
+TTAAGTGCACTCAAATAATGTGATTTTATGAGGCTATAGGAGAAAAAAATT
++
+fdddZ`dc[cdadJ_ddScad[[^\^ddadad__daa^a^\]QY\T^ZZZY
b
diff -r 654b3a274ed5 -r 1ee282794de3 test-data/pairend_strandspecific_51mer_hg19_chr1_1-100000.R1.fastq.gz
b
Binary file test-data/pairend_strandspecific_51mer_hg19_chr1_1-100000.R1.fastq.gz has changed
b
diff -r 654b3a274ed5 -r 1ee282794de3 test-data/pairend_strandspecific_51mer_hg19_chr1_1-100000.R2.fastq
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/pairend_strandspecific_51mer_hg19_chr1_1-100000.R2.fastq Sun Mar 20 10:51:09 2022 +0000
[
@@ -0,0 +1,80 @@
+@seq.11990047/2
+CCCGTGGGCAGAGCAAAGGAAGGGCACAGCGCCAGGCAGTGGTGCAGCTGC
++
+hfadfddd`]f[fa_fh]hhcagffhWhhhh]eeehhhha^hfhhhhghhf
+@seq.14614493/2
+TCCCCTCCCAAGGAAGTAGGTCTGAGCAGCTTGTCCTGGCTGTGTCCATGT
++
+hhhhhhhhhehhhhehehhhghhehfhahhdhfdhhfhhhdf]f_ffbdfa
+@seq.24018133/2
+TACCACTATTTTATGAACCAAGTATAACAAGATACTTGAAATGGAAACTAT
++
+fLffddhhhhag_gefafffhaefhfffffchffhhggahhhhRhhgccgh
+@seq.10608403/2
+GGGACCTGCTGTTCCATCGGCTCTCTCTTGCTGATGGACAAGGGGGCATCA
++
+hefchhhfda`]]b]a^aLa^[Za^WdWb[faff]fd]defQacffdRd]f
+@seq.10820209/2
+GCCCGGGGAAAACATGCATCACAGTTCATCTCGAGTCAGCAGGATTTTGAC
++
+ffcddeed]eaTfccfffffceee]ffdcdcee[efdaffffdSfhhdc]d
+@seq.1537155/2
+TNNATCAATCAGCAGGNNNCGTGCACTCTCTTTGAGCCACCACAGAAAACA
++
+VBBVT^WZ^^I[]V]YBBBIVS[W[eeKceccaccUfaffff_afghg`gd
+@seq.25274725/2
+GGCNCCTCCNTGCCCTNCTNAAAANNCAATCACAGCTCCCTAACAGTCCTG
++
+^UZB]]]Y]B][IS[[B]]BW][XBB\WaadddddhhghgffffaGVV[Se
+@seq.26326595/2
+CATGCGTGCCCTGCTCGANATCCAATCACAGCTCCCTAACACTCCTGAATC
++
+^K^K_YT[YVe_eLe[INBTYZUV^S`babhacfhhccghhahghhdaghW
+@seq.28833653/2
+CAGCAGCTATTTCCTGNTNACTCAANCAATGGCCCCATTTCCCTGGTGGAA
++
+fLdYfeYdddXbbabSBWBTY[[[]BdeedfffffghhhghdfgLfbfddg
+@seq.25049090/2
+CTNCCCTTANTCCGAANGCNGCTCNNCTGATTGGTTAATTTTTGCGTAGCT
++
+VVBNZT[]]BSHZWS[BZHBTQPOBBZUZO]bZ^^hfehffff[fcfd_]g
+@seq.23476912/2
+TNACTGATTNCTCTCCACTNTAGANNCTGAGAAGCCCACGCTGTTCATGCT
++
+`Ba_a^]^\Bbab\aa`b`BV]^VBBZV[Z`a^abffYfaa^e^dedbdd]
+@seq.28059536/2
+CGTNTGACTCTAGACCNTNNGAAGCCCACGCGGTTCATTCTAGCAAGTAAC
++
+ZXZBY`\`]][dcKcUBVBBWNVV]ghchfdcc]ecccLa`edecf_cfdf
+@seq.13270875/2
+TAGATTATCAACAGGGGAGAGATAGCATTTCCTGAAGGCTTCCTAGGTGCC
++
+eghggd_hhhfahhg\K^[[ffafchehg_ffWffhgceghhhhhffLfcY
+@seq.2214586/2
+GNNTGCANANATAGANNTTNCCACACTGCCTTGCACAGGAGCACTGCGGGG
++
+VBBSITZBVBTTRHXBBZYBVUUVHH[QV[chhghacKaa_eeeeghgghg
+@seq.31061198/2
+GNCGGAAAAAAAAATTNNNNAAAATNCGTCTGCTATCAGGGAGTTTTATGA
++
+VB[NV^\_]_`hfccXBBBBTUT[TBa_aLTRNQQaYcaeaKa^adcS\Vd
+@seq.13539256/2
+CCTATTTTTTTTTTTTTTTTTGACACAGGTTCTCTGTCACCCAGCCTGGGG
++
+hhhhhhhhhhhghhhghhghfccWKVVYZRd[_[aZQYZ^``WT`[L^^Q\
+@seq.13835843/2
+TCCATCTTTTTTTTTTTTTTTTTGACACAGGTTCTCTGTCACCCAGCCTGG
++
+ghhhggffhhhhhhhhhhhghhh]fMPLPVW^^WXUZ\WXL[VVJY`\Wbb
+@seq.5556605/2
+GCAGCTANGNCCATCNNNTTTGAAANCCAGATTTCGTTTTAAACCAGAGGA
++
+fLfLf]VBTBI]]]]BBB[^WW[]XBdede`eedeffhhehffhhhhahee
+@seq.20367385/2
+ATTTGGCAGAGAAGCAAACACCAGTCGGAGAGCTGGGGCCCTCCCAGCCCT
++
+W_\_^W___WdcfceVIW[T^aa\[aaacaQYYSZY`KK````^[GaQZ\Y
+@seq.17373919/2
+TTTTTGTTTTTTTTTTTTTTTGAGTCAGAATCTCGCTCTGTTGCCCAGGCT
++
+hgghhhhhhSfffffhgh`h__Wb`ZZZ_]PVPUSVYVQVaWWacaQa^BB
b
diff -r 654b3a274ed5 -r 1ee282794de3 test-data/pairend_strandspecific_51mer_hg19_chr1_1-100000.R2.fastq.gz
b
Binary file test-data/pairend_strandspecific_51mer_hg19_chr1_1-100000.R2.fastq.gz has changed
b
diff -r 654b3a274ed5 -r 1ee282794de3 test-data/prinseq_good_sequences.fastq
--- a/test-data/prinseq_good_sequences.fastq Wed Jul 07 09:22:27 2021 +0000
+++ /dev/null Thu Jan 01 00:00:00 1970 +0000
b
b'@@ -1,13608 +0,0 @@\n-@HWI-M00234:263:000000000-ADM55:1:1101:7508:4067 1:N:0:ATCACG\n-GGTGCACTAGGATCGTAGTTGGCTACTTTCCCGTTTTCAATGTATACGCAAGGTACACGGTCAGCGGTAGCTGCCATAATGTAATGGTAGTCAAATCCTAAGTCTCCCAATGCACATGGCAGTGGAGCGTTCCAATCTTGTGTACCGGCTTTGTCTCCTAATCCCAAGTGCCATTTTCCGATAGCTCCGGTAGCATAACCGGTACTTTTGAACATATCCGCCATGGTATATTGTTCGGGACGGATAATCATGCCGGCATTTCCGGCAGCTACGTCTGTTCCTTTTTTGCGCCATGCATATTCTCCTG\n-+HWI-M00234:263:000000000-ADM55:1:1101:7508:4067 1:N:0:ATCACG\n-CCCCCGFGED8DDCAFDAEE9DFGGGG9CFAFFCC@@CFGFGGCGFGG>GGGFFGDGEFFEFG8>4GF@@FGCFGGCFCFCFFCFG>9=FFGFFGGGGFFFGGGGGFFF=FG<GGGF<F8C=@AC<GDCC:EGGGCCF<FFFFEFGFCGGGGGGD<EGFD;=EFFFGFGGGGAFGGGGGGC>>@DCFGGEFEGFFGF>AFFE>C<DFEFFFFEF8B4DGF9FFEFGGEFDE=GGEEECEFEFF<<FCCDGG<GGGCAGGCEGGFF9EC8FGDGGGGGFFCCDDF:FCGGGGEF9GEFGFCE>CCCB9\n-@HWI-M00234:263:000000000-ADM55:1:1101:26232:4161 1:N:0:ATCACG\n-CTCTTCTTAAGCTCGAACAGCTTCTTCAGTCTTACCTGCCATTTCATCTAAAATTTTTAAAGCTCGCTCTAAAACAGTAGTATCATCAATCATAACCAATCCACCATCTGGTCCAGGTTCCAAGTGAATCCCAACACTTTTCCCCTCCTTAAAATTGTGTCCACCGAAAAAATTTCTTACAGTGTCAACATTCAGTCCAAGTTCATCGGCTATTCTATGCATACTACCACTAGGCAATGAGTCTTTAATCTTACGTAATTCATTAAATGTTATTGTTCTCATATTCATAAATTTAATGGTTAATA\n-+HWI-M00234:263:000000000-ADM55:1:1101:26232:4161 1:N:0:ATCACG\n-CCCCCGGFGFFFGEGGGGCFGGGEGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCFGGF=EGFGGGGFGFGFGGGFAFDGCFGG=GFGGGFGFGFGGGGGGGGFGGGG?FFGGFDFFFGGGGGFGGGGGGCE=GC@FGGGGGGGFGGGGGGFBDFGGCGGGGFFFGGGGGGGGDFGGFFGFDGGGGFFFGGGGF>GFFFAGGFFFFFFGGGGGFEEFFFFFFEFE@GGGGGGGGGFGGGGGGGFGFFGGGGGGGGGGGFGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGGGCCCCC\n-@HWI-M00234:263:000000000-ADM55:1:1101:11727:5103 1:N:0:ATCACG\n-CTCCCGTATAGGATGGATCGAAAGCAATCGTCAGGAATCCACGTTCTGCCAATGTCTGCGCATACAAGCCCGAAGCTTGTTCCTTCACGGCACCAAATGGGCCACTGATGGCAATGGCAGGCAATTTACCCGTCACATTCTTGGGTACATACAAATCGGCGGCAAGTGTAATGCCGTAACGGTTGTGGAAAGTTATCTTTTTATGGTCTACTTTATCACTCTGTGGGAATGTCTTGTCCCACTCTTGAGTCAGATTCAAATTCTCTTCCATAAATTTATTATCATTATTAGGATTATTTTCTGTTGCAGACAAATGTCCTGCAGCACAAAGGCTAATAACCATCGCCGCACATATGTTTT\n-+HWI-M00234:263:000000000-ADM55:1:1101:11727:5103 1:N:0:ATCACG\n-CCCCCGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGGGGEGGGEGGGGGGGEGEEGGGGGGGGGGGGGGGGGGGGGEFFGGGGGGGGGFGGGEGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFFGG>GGGGGGGGGGGFGGGGF@GGGGGGGGGGGGDCFGFFGGFGFFEFFFGFAFGFCGDCFGGGGGGGGDEGGFFGGGGGGEFGGGGGGFGFGGFCGGFGGGFFFFAFGGGGGGGGGGGGGG@GGGGGGGFGGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGGGGGGFFGDGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCCCCC\n-@HWI-M00234:263:000000000-ADM55:1:1101:15029:5173 1:N:0:ATCACG\n-GGCCTATTCTCTGCGGCCGCCTTTCGGCGGCACCCCTTCTCCCGAAGTTACGGGGTCATTTTGCCGAGTTCCTTAACAATGCTTCTTCCGTCGGCCTTAGGATTCTCTCCTCATCCACCTGTGTCGGTTTACGGTACGGGTACAATATAAACAATAGCGGCTTTTCTTGACGGCCGGCTCATGGGCTTCCCTACTTTAGTTCGGTCCGCATCACGCCTTCGGATTGTTCAGCGGATTTGCCTACTGAACTCCTACCTCGCTTGCACCGGATTTTCCGTTTCCGGCTCCCACTTTCCTTCCGGGTCCCCACAGTTCTGTTATATTGCAGTACAGGAATCTCAACCTGTTGTCCATCGGCTACGGCTTTCGCCCTCGCCTTAGGCCCCGACTTACCCAGAGCAGATCAGCTTTACTCTGGAAACCTTAGATATTCGGCCAAAA\n-+HWI-M00234:263:000000000-ADM55:1:1101:15029:5173 1:N:0:ATCACG\n-CCCCCGGGGGGGGGGGGGGGGGGGGEGG7FECGFGGGGGEGGGGGEEGGGGGGGGGGGGGGGFGGGGGGGGGGGGFGGGCGGGGGGGGGGGGGGGFFGGGGGGGGGGFFG9FCGGGFGGGGGFFFDGGEECGGGGGGGGGGGEGGGGGGGGECEGEEGD>ACG>=98E59=CBDDD4D3BGF=FGGGDGFFFFFFFF6BDEFD<88>>DF:C7??3GBB7?:1:@FADC9<C>D>GFFAAF>7:7<4FGCD?AFGGC><9:?2<FECCA,@*;*CDCC@+E@EE>FFFGGDDCGE6C6@6+++GCCE,BCED@B9DFCFCFFEEF,9FGGE9GFF9DFGGGGFGGGGGGGCFFFGGGEGCGGFGFGGGGGGCDDGGGGFEGGGGGGGFGFEFEGGGGCGGGGGGGGCEAGFFGGGGCGGGEGFFCFGECGGCGGDGCC?CC\n-@HWI-M00234:263:000000000-ADM55:1:1101:11799:5476 1:N:0:ATCACG\n-CCTAAATTTTCGAGTATAAGCAGGACCGAAGCCACCAACTTATACCTTATCCTCTAAATTTTCGTAAGAGACATGAAGCGGATCTCTAACTATTCCCGATTTTACTGCACCACTTGATCAAACGCTTCGGAACAAGAGCTTTTGAGTGATGTCTCGTTTCCACCACTTTGGCAGAAATAAAGCTAATCTACTATGATTCGATTAAGCAGCAAGAGCATAATTTTCGTTGCCAGATAAAATTTTGAAAACTGGGATTATAGTGCCAATCAACCACGCACTGCATGCTTACATACCACTTAGCCACGCTGTCAAATCCAG\n-+HWI-M00234:263:000000000-ADM55:1:1101:11799:5476 1:N:0:ATCACG\n-CCCC<FFFGGF@FFFGFFGAGCCDGGGEFGGD;CFFGDGGGEGGCFF@DFGGC<CEGGGCEFGEGF@E@@,BFFGFGGGGG7+@AFEAEFDGEGGGGGFFEGGFGGGCEGEFDGFG=FGGGD>CED@DGFFGDCFECCCFDGGGDFGGGGF=DEEEDGFDEGFF@@,==+=FGEGFGG'..b'CFC@C,B@,F<F9GEF7DEF7F@@EEEEGEFC9GGGF<<<A8\n-@HWI-M00234:263:000000000-ADM55:1:2119:14032:23863 1:N:0:ATCACG\n-CGCCCTTCTTTTCCATATAGTTTTTGTAAGTAGCGATGGCTTGTTCGCCCTTACCGTCGCGGCTATACAGTTTAGCCAACTCCTTGTAGTTGTTGAGCTTAGAGGGGTCCTTAGCCAAAGCTTTTTCGTAAGCCGTGATAGCAGCGGGGAAATCCTGCTTTTCCTTAGCCAAAGCAGCAGCATATTCGTAGTCTACCGAAATGTAGTTGCTGTCAGCGTATTCAGGATCGCTGAGGTAAGCCGAAGCTTCTTCAGCCGTTTTGATAGCTGCATCGTAGTCCATAGCTTCGCCAATCTTGTTTACTTTG\n-+HWI-M00234:263:000000000-ADM55:1:2119:14032:23863 1:N:0:ATCACG\n-CCCCCFEFGFDGGCABGCCECEFEGGGFAFFEGGCGGFGGGGC<FCGGFGGGGGFFAF++@7FG7FF*C@FGGC?F4CFGCFEGGAFEEFF<FFGGG=?EFGGDGFFGGFGF<FDBGCF><FEFF<4@EFG@ECFGEFGCEFCC@BD9FECFGGFEEGCAGE?EFEGGGGGEFFGGG,DEEACAFCGFAFFECGGG=GGGFB?GGGGGFFDF@AEGGGGGE30B=BCGGFFFFF3F8FCECCFGGGFDGFE@F7EC>GF@FFE9FGGFC>@,;BAGGGGFECGD@@+FCDGGGFE@GCF<GGGCCCCC\n-@HWI-M00234:263:000000000-ADM55:1:2119:16782:24009 1:N:0:ATCACG\n-CGGTAAATATCTTCTGACTCAACACTTTTGGCAGAAGTTCCGCTATCAGTTCGCCGACGGAAGGTTCCACAATGTAATCATTATTAAAACTGCGGGCATCCTCCGGTACATCTTCTGCCGCCTCCTGCGCTACTTGCGTCAGGTCAATGGGCAGATAATTCTCGCGGAGCAGCACCTGCGAACCCATCGATTTGAAGTGATGGTAAATCAGCTCCACATGGTCAATCTGCTTTTCCACAAACTCATGCATCAACTTCTCTGCCAGCTCATACGCTTCAACGTACGAAGGCTTGTCTGCCATCACCTGGTAGCTGCCTTGCGGCACATATCCCATTTTCTTG\n-+HWI-M00234:263:000000000-ADM55:1:2119:16782:24009 1:N:0:ATCACG\n-CCCCCGFFGAFGCGGAFG;EFGGGGGGGGFCCEFGGGFFGGGEGCFGGGDFGG@7@FGDFEGDGACFFEFGEDFDGGDEF9FD>EFGGFCEFDGFFFEGG=GFGF?CCEF?GG>GGGFDF>E7FGEF8FCCFGGEFFFGGGGGGGGFCFGFCFFGGGGGGGGGG<GGGGGGGGG>CFGGGEGDECFFGGFGGGFFFGFGGGGGGFFCACF=DCFGFFFGFGEBCGFGFFEFFFGGGGF9FACFEAFGFEAAFBFFEB<EFFGCGGFGGEFFCGFGFFFFGFECBFFC:1@CGFF@FFFGFFDEEFEC@CFCGFGF@GECCEFAFE<GGGGFF@@,GCC<CC\n-@HWI-M00234:263:000000000-ADM55:1:2119:11921:24379 1:N:0:ATCACG\n-CCTTTCGTTCTCGTTCCAAAATTTTATCCGGATATTCTTTTTCTCTTATACTTTTTTCTGTTCAGAAGTCAGATATCAATTTTCTCTTATTATTATAAATTATCAATTTAAAAAATTGTTCTCTACACACTCCGCTTACAAAACGCTCATAACCAAAATGTATAGTTGCTTTTTTAAAACTTTTTAACCTTCAAATAACAATTATTCTTGTTACCCTTTTCTGATCTGGTTCTTCATCTTATACTCTTGGCGAGTATATATGAAACTATGTAACAGATTAAAAAAATATCTTTTTGAAATTCCCTTTTTCTTCAGGAACTT\n-+HWI-M00234:263:000000000-ADM55:1:2119:11921:24379 1:N:0:ATCACG\n-BCCCCGGGGGGGGFGGGCD@FGFFGGFFGGDGGGFGAFFG9FFGDFGDGCAFGE9FGEGGGGAFBECFFEEEGGCCDGGGGGGGGGGGGFAFGFGGAE9DFE<FFFFFCGFFGB@EGGGGGE;GFCAB;CEFGGGDGFGGGFEG5GGGGGGGGDGF@F<FDAFFDDEFGGC=@GGGFGFGGGF(DFFGGDCGG<CG9<ECFDGG@FGFA9G<FFGBEFC,EAB7D@FF8:87FEDFF-FFFF0EE86FB6:0<55@4CECCEECC==EE4CEC@F>CC5CEAEGEFGCGCFFEC79DE<C,,,@C6,,6;,6,=,,A--@A\n-@HWI-M00234:263:000000000-ADM55:1:2119:17687:24444 1:N:0:ATCACG\n-CGGCAGGCGTTCTCGGCGTCGTGCACGAGGAACTTCTTGGACTTGATGATGCGCTTGCCGTAAACGGGGTGCATGGCATGCCGGGTCACACGGACAGAGATCGTCTTCTCCATCTTGTCGCTGATAACGATTCCGATTTTCATCTTGCGATGAGGGGTTTTCGCTTCCATTGAGCCTTACCTCCTTCCGGTGTTCACGTTCAGTCCCTGTTCTTTTTCGTGAACAATGGTAAGGACTCTCGCGATGGTCTTCTTGACCTCTTTGATTCTGCTGGTGTTCTTCAACTGGACAACAGCATTCTGGAAGCGCAGGTTAAAAAGCTCTTCTTTGGACTGGTCGTGCTTGTTCATGAGCTC\n-+HWI-M00234:263:000000000-ADM55:1:2119:17687:24444 1:N:0:ATCACG\n-CCCC@@FEGGGGGG7<778FFGFFGC<;CGGGGFEFC,;E8<CACFAD9FFCFGCFGF8@8BC@EF77FFBFEGCFG<<BAFACCFCGFGGG@>+CFFC:,?=:F8AD,BED=<,CECAC@FGE*E,A>+3@CD@7@=FEG7=;@CDEC)63@FGEC:BC@E>?CDEGFGG@FGDFECFGCEEEDCCCBEEDGGGF@A38@EB@FGFFEDFGCECCFAC?GEGF=@FEFGGFDEE:FF>69@9584<GFFF9F=F8DGF9GGFGGFFE<<6B6F<BFFCFF<FEC<::*,FEFFF<E<<CFFB,EDGEFFGGFC,<<,9EE<CFFF@FEC,GFEF:B@F8FFFFFFC,<FC,CCCA\n-@HWI-M00234:263:000000000-ADM55:1:2119:12876:24965 1:N:0:ATCACG\n-CTAAATTTTCGAGTATAAGCAGGACCGAAGCCACCAACTTATACCTTATCCTCTAAATTTTCGTAAGAGACATGAAGCGGATCTCTAACTATTCCCGATTTTACTGCACCACTTGATCAAACGCTTCGGAACAAGAGCTTTTGAGTGATGTCTCGTTTCCACCACTTTGGCAGAAATAAAGCTAATCTACTATGATTCGATTAAGCAGCAAGAGCATAATTTTCGTTGCCAGATAAAATTTTGAAAACTGGGATTATAGTGCCAATCAACCACGCACTGCATGCTTACATACCACTTAGCCACGCTGTCAAATCCAG\n-+HWI-M00234:263:000000000-ADM55:1:2119:12876:24965 1:N:0:ATCACG\n-CCCCCGGGGGGGGGGGGGFGGGGEGCFCGGGGEFGGGGFGGGGGDCFCFFFGGFFFDFEGGEGGGGGGGGGGGGGGFFGG>FFGGFFFGGGGDEFGEDFGAFEFFAFAFGGGGGD@BCEEEFGGE@BFFECEEGGGGGGFFFCGGGGFGCFFFAEFGFGGFGGGGGGGGGGFEDFF9AECFGFGGGGGGGGGFG@CFFGGGGGGFEGF?CFGFAGGFFFBGEFFCGFGGGDECFFGEGFFFFAGFGGGFFEGGF@GFFGFGCGEFEDGFFFF<GGGFCGFGGGGGFFFGGGGGFECDFEE7EEFGDFF9AADCCC@@\n'
b
diff -r 654b3a274ed5 -r 1ee282794de3 test-data/prinseq_input_sequences.fastq
--- a/test-data/prinseq_input_sequences.fastq Wed Jul 07 09:22:27 2021 +0000
+++ /dev/null Thu Jan 01 00:00:00 1970 +0000
b
b'@@ -1,13812 +0,0 @@\n-@HWI-M00234:263:000000000-ADM55:1:1101:7508:4067 1:N:0:ATCACG\n-GGTGCACTAGGATCGTAGTTGGCTACTTTCCCGTTTTCAATGTATACGCAAGGTACACGGTCAGCGGTAGCTGCCATAATGTAATGGTAGTCAAATCCTAAGTCTCCCAATGCACATGGCAGTGGAGCGTTCCAATCTTGTGTACCGGCTTTGTCTCCTAATCCCAAGTGCCATTTTCCGATAGCTCCGGTAGCATAACCGGTACTTTTGAACATATCCGCCATGGTATATTGTTCGGGACGGATAATCATGCCGGCATTTCCGGCAGCTACGTCTGTTCCTTTTTTGCGCCATGCATATTCTCCTG\n-+\n-CCCCCGFGED8DDCAFDAEE9DFGGGG9CFAFFCC@@CFGFGGCGFGG>GGGFFGDGEFFEFG8>4GF@@FGCFGGCFCFCFFCFG>9=FFGFFGGGGFFFGGGGGFFF=FG<GGGF<F8C=@AC<GDCC:EGGGCCF<FFFFEFGFCGGGGGGD<EGFD;=EFFFGFGGGGAFGGGGGGC>>@DCFGGEFEGFFGF>AFFE>C<DFEFFFFEF8B4DGF9FFEFGGEFDE=GGEEECEFEFF<<FCCDGG<GGGCAGGCEGGFF9EC8FGDGGGGGFFCCDDF:FCGGGGEF9GEFGFCE>CCCB9\n-@HWI-M00234:263:000000000-ADM55:1:1101:26232:4161 1:N:0:ATCACG\n-CTCTTCTTAAGCTCGAACAGCTTCTTCAGTCTTACCTGCCATTTCATCTAAAATTTTTAAAGCTCGCTCTAAAACAGTAGTATCATCAATCATAACCAATCCACCATCTGGTCCAGGTTCCAAGTGAATCCCAACACTTTTCCCCTCCTTAAAATTGTGTCCACCGAAAAAATTTCTTACAGTGTCAACATTCAGTCCAAGTTCATCGGCTATTCTATGCATACTACCACTAGGCAATGAGTCTTTAATCTTACGTAATTCATTAAATGTTATTGTTCTCATATTCATAAATTTAATGGTTAATA\n-+\n-CCCCCGGFGFFFGEGGGGCFGGGEGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCFGGF=EGFGGGGFGFGFGGGFAFDGCFGG=GFGGGFGFGFGGGGGGGGFGGGG?FFGGFDFFFGGGGGFGGGGGGCE=GC@FGGGGGGGFGGGGGGFBDFGGCGGGGFFFGGGGGGGGDFGGFFGFDGGGGFFFGGGGF>GFFFAGGFFFFFFGGGGGFEEFFFFFFEFE@GGGGGGGGGFGGGGGGGFGFFGGGGGGGGGGGFGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGGGCCCCC\n-@HWI-M00234:263:000000000-ADM55:1:1101:11727:5103 1:N:0:ATCACG\n-CTCCCGTATAGGATGGATCGAAAGCAATCGTCAGGAATCCACGTTCTGCCAATGTCTGCGCATACAAGCCCGAAGCTTGTTCCTTCACGGCACCAAATGGGCCACTGATGGCAATGGCAGGCAATTTACCCGTCACATTCTTGGGTACATACAAATCGGCGGCAAGTGTAATGCCGTAACGGTTGTGGAAAGTTATCTTTTTATGGTCTACTTTATCACTCTGTGGGAATGTCTTGTCCCACTCTTGAGTCAGATTCAAATTCTCTTCCATAAATTTATTATCATTATTAGGATTATTTTCTGTTGCAGACAAATGTCCTGCAGCACAAAGGCTAATAACCATCGCCGCACATATGTTTT\n-+\n-CCCCCGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGGGGEGGGEGGGGGGGEGEEGGGGGGGGGGGGGGGGGGGGGEFFGGGGGGGGGFGGGEGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFFGG>GGGGGGGGGGGFGGGGF@GGGGGGGGGGGGDCFGFFGGFGFFEFFFGFAFGFCGDCFGGGGGGGGDEGGFFGGGGGGEFGGGGGGFGFGGFCGGFGGGFFFFAFGGGGGGGGGGGGGG@GGGGGGGFGGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGGGGGGFFGDGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCCCCC\n-@HWI-M00234:263:000000000-ADM55:1:1101:15029:5173 1:N:0:ATCACG\n-GGCCTATTCTCTGCGGCCGCCTTTCGGCGGCACCCCTTCTCCCGAAGTTACGGGGTCATTTTGCCGAGTTCCTTAACAATGCTTCTTCCGTCGGCCTTAGGATTCTCTCCTCATCCACCTGTGTCGGTTTACGGTACGGGTACAATATAAACAATAGCGGCTTTTCTTGACGGCCGGCTCATGGGCTTCCCTACTTTAGTTCGGTCCGCATCACGCCTTCGGATTGTTCAGCGGATTTGCCTACTGAACTCCTACCTCGCTTGCACCGGATTTTCCGTTTCCGGCTCCCACTTTCCTTCCGGGTCCCCACAGTTCTGTTATATTGCAGTACAGGAATCTCAACCTGTTGTCCATCGGCTACGGCTTTCGCCCTCGCCTTAGGCCCCGACTTACCCAGAGCAGATCAGCTTTACTCTGGAAACCTTAGATATTCGGCCAAAA\n-+\n-CCCCCGGGGGGGGGGGGGGGGGGGGEGG7FECGFGGGGGEGGGGGEEGGGGGGGGGGGGGGGFGGGGGGGGGGGGFGGGCGGGGGGGGGGGGGGGFFGGGGGGGGGGFFG9FCGGGFGGGGGFFFDGGEECGGGGGGGGGGGEGGGGGGGGECEGEEGD>ACG>=98E59=CBDDD4D3BGF=FGGGDGFFFFFFFF6BDEFD<88>>DF:C7??3GBB7?:1:@FADC9<C>D>GFFAAF>7:7<4FGCD?AFGGC><9:?2<FECCA,@*;*CDCC@+E@EE>FFFGGDDCGE6C6@6+++GCCE,BCED@B9DFCFCFFEEF,9FGGE9GFF9DFGGGGFGGGGGGGCFFFGGGEGCGGFGFGGGGGGCDDGGGGFEGGGGGGGFGFEFEGGGGCGGGGGGGGCEAGFFGGGGCGGGEGFFCFGECGGCGGDGCC?CC\n-@HWI-M00234:263:000000000-ADM55:1:1101:11799:5476 1:N:0:ATCACG\n-CCTAAATTTTCGAGTATAAGCAGGACCGAAGCCACCAACTTATACCTTATCCTCTAAATTTTCGTAAGAGACATGAAGCGGATCTCTAACTATTCCCGATTTTACTGCACCACTTGATCAAACGCTTCGGAACAAGAGCTTTTGAGTGATGTCTCGTTTCCACCACTTTGGCAGAAATAAAGCTAATCTACTATGATTCGATTAAGCAGCAAGAGCATAATTTTCGTTGCCAGATAAAATTTTGAAAACTGGGATTATAGTGCCAATCAACCACGCACTGCATGCTTACATACCACTTAGCCACGCTGTCAAATCCAG\n-+\n-CCCC<FFFGGF@FFFGFFGAGCCDGGGEFGGD;CFFGDGGGEGGCFF@DFGGC<CEGGGCEFGEGF@E@@,BFFGFGGGGG7+@AFEAEFDGEGGGGGFFEGGFGGGCEGEFDGFG=FGGGD>CED@DGFFGDCFECCCFDGGGDFGGGGF=DEEEDGFDEGFF@@,==+=FGEGFGGGGGGGGGGFFGFFGFFFFFFFGGGFGFFFEFFFFA8A@GGGFFEGGGFEGEAFGFFGGCEAE>CGFFFF<F@CFFGEAGFEDEGDEEGGGGFCFFCE>CCGC?GDGFFGGGGGGFEFF,FDGDGFGFFE,GDEE<<CCC@\n-@HWI-M00234:263:000000000-ADM55:1:1101:11348:5668 1:N:0:ATCACG\n-GCCTAAATTTTCGTGCAGCGGCAGAACCAAAGCCATCAACCGTTGCCTTATCCTCTAAATTTTCACCCGCGCCACGAGGCTGACACGAACTATCCCCG'..b'GFGF@FGE7FCCEFGCGFGFGFFG@CFFGCGGFFGAF9FDDFDF<B<<FEEEGGC9B?FFCGF,BEBF<?DEFEGGECC@FGFFGGGF=F?DGGGECFGFGEGGGG;7A3@,AD7FGFF997CD>=@DDDC87DCEE;535$F@785CCFF@<ACE:=9E<CFGGGGEDCFCGGGCFGFDFE9;@:3(CCB9FGFF@FCECEF<DEFFFFFGFCCAFEA=<?EA<ECGFGFFFA(EFA?AC:EC><?7CGF@CAF?B:C+FEF:GGEECAEAC,<CFE@CCFECFFCFE<@FFF@+E@9FCCF8C:FCEACFC@C,B@,F<F9GEF7DEF7F@@EEEEGEFC9GGGF<<<A8\n-@HWI-M00234:263:000000000-ADM55:1:2119:14032:23863 1:N:0:ATCACG\n-CGCCCTTCTTTTCCATATAGTTTTTGTAAGTAGCGATGGCTTGTTCGCCCTTACCGTCGCGGCTATACAGTTTAGCCAACTCCTTGTAGTTGTTGAGCTTAGAGGGGTCCTTAGCCAAAGCTTTTTCGTAAGCCGTGATAGCAGCGGGGAAATCCTGCTTTTCCTTAGCCAAAGCAGCAGCATATTCGTAGTCTACCGAAATGTAGTTGCTGTCAGCGTATTCAGGATCGCTGAGGTAAGCCGAAGCTTCTTCAGCCGTTTTGATAGCTGCATCGTAGTCCATAGCTTCGCCAATCTTGTTTACTTTG\n-+\n-CCCCCFEFGFDGGCABGCCECEFEGGGFAFFEGGCGGFGGGGC<FCGGFGGGGGFFAF++@7FG7FF*C@FGGC?F4CFGCFEGGAFEEFF<FFGGG=?EFGGDGFFGGFGF<FDBGCF><FEFF<4@EFG@ECFGEFGCEFCC@BD9FECFGGFEEGCAGE?EFEGGGGGEFFGGG,DEEACAFCGFAFFECGGG=GGGFB?GGGGGFFDF@AEGGGGGE30B=BCGGFFFFF3F8FCECCFGGGFDGFE@F7EC>GF@FFE9FGGFC>@,;BAGGGGFECGD@@+FCDGGGFE@GCF<GGGCCCCC\n-@HWI-M00234:263:000000000-ADM55:1:2119:16782:24009 1:N:0:ATCACG\n-CGGTAAATATCTTCTGACTCAACACTTTTGGCAGAAGTTCCGCTATCAGTTCGCCGACGGAAGGTTCCACAATGTAATCATTATTAAAACTGCGGGCATCCTCCGGTACATCTTCTGCCGCCTCCTGCGCTACTTGCGTCAGGTCAATGGGCAGATAATTCTCGCGGAGCAGCACCTGCGAACCCATCGATTTGAAGTGATGGTAAATCAGCTCCACATGGTCAATCTGCTTTTCCACAAACTCATGCATCAACTTCTCTGCCAGCTCATACGCTTCAACGTACGAAGGCTTGTCTGCCATCACCTGGTAGCTGCCTTGCGGCACATATCCCATTTTCTTG\n-+\n-CCCCCGFFGAFGCGGAFG;EFGGGGGGGGFCCEFGGGFFGGGEGCFGGGDFGG@7@FGDFEGDGACFFEFGEDFDGGDEF9FD>EFGGFCEFDGFFFEGG=GFGF?CCEF?GG>GGGFDF>E7FGEF8FCCFGGEFFFGGGGGGGGFCFGFCFFGGGGGGGGGG<GGGGGGGGG>CFGGGEGDECFFGGFGGGFFFGFGGGGGGFFCACF=DCFGFFFGFGEBCGFGFFEFFFGGGGF9FACFEAFGFEAAFBFFEB<EFFGCGGFGGEFFCGFGFFFFGFECBFFC:1@CGFF@FFFGFFDEEFEC@CFCGFGF@GECCEFAFE<GGGGFF@@,GCC<CC\n-@HWI-M00234:263:000000000-ADM55:1:2119:11921:24379 1:N:0:ATCACG\n-CCTTTCGTTCTCGTTCCAAAATTTTATCCGGATATTCTTTTTCTCTTATACTTTTTTCTGTTCAGAAGTCAGATATCAATTTTCTCTTATTATTATAAATTATCAATTTAAAAAATTGTTCTCTACACACTCCGCTTACAAAACGCTCATAACCAAAATGTATAGTTGCTTTTTTAAAACTTTTTAACCTTCAAATAACAATTATTCTTGTTACCCTTTTCTGATCTGGTTCTTCATCTTATACTCTTGGCGAGTATATATGAAACTATGTAACAGATTAAAAAAATATCTTTTTGAAATTCCCTTTTTCTTCAGGAACTT\n-+\n-BCCCCGGGGGGGGFGGGCD@FGFFGGFFGGDGGGFGAFFG9FFGDFGDGCAFGE9FGEGGGGAFBECFFEEEGGCCDGGGGGGGGGGGGFAFGFGGAE9DFE<FFFFFCGFFGB@EGGGGGE;GFCAB;CEFGGGDGFGGGFEG5GGGGGGGGDGF@F<FDAFFDDEFGGC=@GGGFGFGGGF(DFFGGDCGG<CG9<ECFDGG@FGFA9G<FFGBEFC,EAB7D@FF8:87FEDFF-FFFF0EE86FB6:0<55@4CECCEECC==EE4CEC@F>CC5CEAEGEFGCGCFFEC79DE<C,,,@C6,,6;,6,=,,A--@A\n-@HWI-M00234:263:000000000-ADM55:1:2119:17687:24444 1:N:0:ATCACG\n-CGGCAGGCGTTCTCGGCGTCGTGCACGAGGAACTTCTTGGACTTGATGATGCGCTTGCCGTAAACGGGGTGCATGGCATGCCGGGTCACACGGACAGAGATCGTCTTCTCCATCTTGTCGCTGATAACGATTCCGATTTTCATCTTGCGATGAGGGGTTTTCGCTTCCATTGAGCCTTACCTCCTTCCGGTGTTCACGTTCAGTCCCTGTTCTTTTTCGTGAACAATGGTAAGGACTCTCGCGATGGTCTTCTTGACCTCTTTGATTCTGCTGGTGTTCTTCAACTGGACAACAGCATTCTGGAAGCGCAGGTTAAAAAGCTCTTCTTTGGACTGGTCGTGCTTGTTCATGAGCTC\n-+\n-CCCC@@FEGGGGGG7<778FFGFFGC<;CGGGGFEFC,;E8<CACFAD9FFCFGCFGF8@8BC@EF77FFBFEGCFG<<BAFACCFCGFGGG@>+CFFC:,?=:F8AD,BED=<,CECAC@FGE*E,A>+3@CD@7@=FEG7=;@CDEC)63@FGEC:BC@E>?CDEGFGG@FGDFECFGCEEEDCCCBEEDGGGF@A38@EB@FGFFEDFGCECCFAC?GEGF=@FEFGGFDEE:FF>69@9584<GFFF9F=F8DGF9GGFGGFFE<<6B6F<BFFCFF<FEC<::*,FEFFF<E<<CFFB,EDGEFFGGFC,<<,9EE<CFFF@FEC,GFEF:B@F8FFFFFFC,<FC,CCCA\n-@HWI-M00234:263:000000000-ADM55:1:2119:12876:24965 1:N:0:ATCACG\n-CTAAATTTTCGAGTATAAGCAGGACCGAAGCCACCAACTTATACCTTATCCTCTAAATTTTCGTAAGAGACATGAAGCGGATCTCTAACTATTCCCGATTTTACTGCACCACTTGATCAAACGCTTCGGAACAAGAGCTTTTGAGTGATGTCTCGTTTCCACCACTTTGGCAGAAATAAAGCTAATCTACTATGATTCGATTAAGCAGCAAGAGCATAATTTTCGTTGCCAGATAAAATTTTGAAAACTGGGATTATAGTGCCAATCAACCACGCACTGCATGCTTACATACCACTTAGCCACGCTGTCAAATCCAG\n-+\n-CCCCCGGGGGGGGGGGGGFGGGGEGCFCGGGGEFGGGGFGGGGGDCFCFFFGGFFFDFEGGEGGGGGGGGGGGGGGFFGG>FFGGFFFGGGGDEFGEDFGAFEFFAFAFGGGGGD@BCEEEFGGE@BFFECEEGGGGGGFFFCGGGGFGCFFFAEFGFGGFGGGGGGGGGGFEDFF9AECFGFGGGGGGGGGFG@CFFGGGGGGFEGF?CFGFAGGFFFBGEFFCGFGGGDECFFGEGFFFFAGFGGGFFEGGF@GFFGFGCGEFEDGFFFF<GGGFCGFGGGGGFFFGGGGGFECDFEE7EEFGDFF9AADCCC@@\n'
b
diff -r 654b3a274ed5 -r 1ee282794de3 test-data/prinseq_input_sequences.fastq.gz
b
Binary file test-data/prinseq_input_sequences.fastq.gz has changed