Repository 'fastx_nucleotides_distribution'
hg clone https://toolshed.g2.bx.psu.edu/repos/devteam/fastx_nucleotides_distribution

Changeset 4:21796f46e0ba (2018-05-08)
Previous changeset 3:18a51655db17 (2015-11-11) Next changeset 5:ad116ae3f4d9 (2020-01-30)
Commit message:
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/fastx_toolkit/fastx_nucleotides_distribution commit bbb2e6b6769b03602a8ab97001f88fbec52080a1
modified:
fastx_nucleotides_distribution.xml
added:
macros.xml
test-data/fastq_stats1.out
test-data/fastx_nt_distribution.png
test-data/fastx_nucleotides_distribution-in1.txt
test-data/fastx_nucleotides_distribution-out1.png
removed:
tool_dependencies.xml
b
diff -r 18a51655db17 -r 21796f46e0ba fastx_nucleotides_distribution.xml
--- a/fastx_nucleotides_distribution.xml Wed Nov 11 12:39:18 2015 -0500
+++ b/fastx_nucleotides_distribution.xml Tue May 08 13:27:48 2018 -0400
[
@@ -1,19 +1,28 @@
-<tool id="cshl_fastx_nucleotides_distribution" version="1.0.0" name="Draw nucleotides distribution chart">
- <description></description>
-    <requirements>
-        <requirement type="package" version="0.0.13">fastx_toolkit</requirement>
-    </requirements>
- <command>fastx_nucleotide_distribution_graph.sh -t '$input.name' -i $input -o $output</command>
-
- <inputs>
- <param format="txt" name="input" type="data" label="Statistics Text File" help="output of 'FASTX Statistics' tool" />
- </inputs>
-
- <outputs>
- <data format="png" name="output" metadata_source="input" />
- </outputs>
-<help>
-
+<tool id="cshl_fastx_nucleotides_distribution" version="1.0.1" name="Draw nucleotides distribution chart">
+    <description></description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements" />
+    <command detect_errors="exit_code"><![CDATA[
+fastx_nucleotide_distribution_graph.sh
+-t '$input.name'
+-i '$input'
+-o '$output'
+    ]]></command>
+    <inputs>
+        <param name="input" type="data" format="txt" label="Statistics text file" help="Output of 'FASTX Statistics' tool" />
+    </inputs>
+    <outputs>
+        <data name="output" format="png" metadata_source="input" />
+    </outputs>
+    <tests>
+        <test>
+            <param name="input" value="fastx_nucleotides_distribution-in1.txt" />
+            <output name="output" file="fastx_nucleotides_distribution-out1.png" compare="sim_size" delta="512" />
+        </test>
+    </tests>
+    <help><![CDATA[
 **What it does**
 
 Creates a stacked-histogram graph for the nucleotide distribution in the Solexa library.
@@ -29,7 +38,7 @@
 The following chart clearly shows the barcode used at the 5'-end of the library: **GATCT**
 
 .. image:: fastq_nucleotides_distribution_1.png

+
 In the following chart, one can almost 'read' the most abundant sequence by looking at the dominant values: **TGATA TCGTA TTGAT GACTG AA...**
 
 .. image:: fastq_nucleotides_distribution_2.png
@@ -47,7 +56,7 @@
 This tool is based on `FASTX-toolkit`__ by Assaf Gordon.
 
  .. __: http://hannonlab.cshl.edu/fastx_toolkit/
-
-</help>
-<!-- FASTQ-Nucleotides-Distribution is part of the FASTX-toolkit, by A.Gordon (gordon@cshl.edu) -->
+    ]]></help>
+    <expand macro="citations" />
+    <!-- FASTQ-Nucleotides-Distribution is part of the FASTX-toolkit, by A.Gordon (gordon@cshl.edu) -->
 </tool>
b
diff -r 18a51655db17 -r 21796f46e0ba macros.xml
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/macros.xml Tue May 08 13:27:48 2018 -0400
[
@@ -0,0 +1,54 @@
+<?xml version="1.0"?>
+<macros>
+    <token name="@CATS@">
+        #if $input.is_of_type('fasta.gz', 'fastqsanger.gz', 'fastqsolexa.gz', 'fastqillumina.gz'):
+            zcat -f '$input' |
+        #elif $input.is_of_type('fastqsanger.bz2', 'fastqsolexa.bz2', 'fastqillumina.bz2'):
+            bzcat -f '$input' |
+        #else:
+            cat '$input' |
+        #end if
+    </token>
+    <token name="@FQQUAL@">
+        <![CDATA[
+            #if $input.is_of_type('fastqsanger', 'fastqsanger.gz', 'fastqsanger.bz2'):
+                -Q 33
+            #elif $input.is_of_type('fastqsolexa', 'fastqsolexa.gz', 'fastqsolexa.bz2', 'fastqillumina', 'fastqillumina.gz', 'fastqillumina.bz2'):
+                -Q 64
+            #end if
+        ]]>
+    </token>
+    <xml name="requirements">
+        <requirements>
+            <requirement type="package" version="@VERSION@">fastx_toolkit</requirement>
+            <yield />
+        </requirements>
+    </xml>
+    <token name="@VERSION@">0.0.14</token>
+    <token name="@SANGER@">fastqsanger,fastqsanger.gz,fastqsanger.bz2</token>
+    <token name="@SOLEXA@">fastqsolexa,fastqsolexa.gz,fastqsolexa.bz2</token>
+    <token name="@ILLUMINA@">fastqillumina,fastqillumina.gz,fastqillumina.bz2</token>
+    <token name="@FASTQS@">@SANGER@,@SOLEXA@,@ILLUMINA@</token>
+    <token name="@FASTAS@">fasta,fasta.gz</token>
+    <xml name="citations">
+        <citations>
+            <citation type="bibtex">
+                @UNPUBLISHED{agordon,
+                    author = "Assaf Gordon",
+                    title = "FASTQ/A short-reads pre-processing tools",
+                    year = "2010",
+                    note = "http://hannonlab.cshl.edu/fastx_toolkit/",
+                    url = "http://hannonlab.cshl.edu/fastx_toolkit/"}
+            </citation>
+        </citations>
+    </xml>
+    <xml name="fasta_input">
+        <param name="input" type="data" format="@FASTAS@" label="Input FASTA file" />
+    </xml>
+    <xml name="fastq_input">
+        <param name="input" type="data" format="@FASTQS@" label="Input FASTQ file" />
+    </xml>
+    <xml name="fastx_input">
+        <param name="input" type="data" format="@FASTAS@,@FASTQS@" label="Input file in FASTA or FASTQ format" />
+    </xml>
+</macros>
b
diff -r 18a51655db17 -r 21796f46e0ba test-data/fastq_stats1.out
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/fastq_stats1.out Tue May 08 13:27:48 2018 -0400
b
@@ -0,0 +1,37 @@
+column count min max sum mean Q1 med Q3 IQR lW rW A_Count C_Count G_Count T_Count N_Count Max_count
+1 9 23 34 288 32.00 33 33 33 0 33 33 3 1 4 1 0 9
+2 9 28 33 287 31.89 31 33 33 2 28 33 3 3 2 1 0 9
+3 9 13 34 268 29.78 28 33 33 5 21 34 5 1 0 3 0 9
+4 9 17 33 261 29.00 30 33 33 3 26 33 1 2 3 3 0 9
+5 9 22 33 269 29.89 30 33 33 3 26 33 3 3 3 0 0 9
+6 9 22 33 277 30.78 30 33 33 3 26 33 5 3 0 1 0 9
+7 9 21 33 258 28.67 24 33 33 9 21 33 4 1 3 1 0 9
+8 9 12 33 263 29.22 32 33 33 1 31 33 2 1 1 5 0 9
+9 9 29 33 290 32.22 33 33 33 0 33 33 3 3 2 1 0 9
+10 9 23 33 277 30.78 32 33 33 1 31 33 1 4 2 2 0 9
+11 9 12 33 245 27.22 21 31 33 12 12 33 5 2 1 1 0 9
+12 9 13 33 214 23.78 15 24 33 18 13 33 2 4 2 1 0 9
+13 9 5 33 249 27.67 29 31 33 4 23 33 2 1 1 5 0 9
+14 9 5 33 233 25.89 24 33 33 9 11 33 3 3 2 1 0 9
+15 9 15 33 251 27.89 24 33 33 9 15 33 5 1 1 2 0 9
+16 9 23 34 269 29.89 24 33 33 9 23 34 3 1 2 3 0 9
+17 9 13 34 266 29.56 33 33 33 0 33 33 2 3 1 3 0 9
+18 9 21 34 272 30.22 31 33 33 2 28 34 0 5 1 3 0 9
+19 9 5 34 244 27.11 27 30 33 6 18 34 4 4 1 0 0 9
+20 9 11 34 241 26.78 23 32 33 10 11 34 3 4 2 0 0 9
+21 9 13 33 240 26.67 24 27 33 9 13 33 1 4 0 4 0 9
+22 9 5 33 190 21.11 13 21 33 20 5 33 1 4 0 3 1 9
+23 9 5 33 205 22.78 16 26 33 17 5 33 4 4 1 0 0 9
+24 9 5 33 247 27.44 28 31 33 5 21 33 1 5 1 2 0 9
+25 9 11 34 241 26.78 24 33 33 9 11 34 3 4 0 2 0 9
+26 9 5 33 212 23.56 18 31 33 15 5 33 0 6 0 3 0 9
+27 9 5 33 227 25.22 21 26 33 12 5 33 3 4 1 1 0 9
+28 9 21 33 255 28.33 24 31 33 9 21 33 2 4 3 0 0 9
+29 9 5 33 228 25.33 21 30 33 12 5 33 2 4 1 2 0 9
+30 9 10 33 213 23.67 16 28 33 17 10 33 3 4 2 0 0 9
+31 9 5 33 236 26.22 21 31 33 12 5 33 1 4 1 3 0 9
+32 9 5 33 210 23.33 12 29 33 21 5 33 3 3 0 3 0 9
+33 9 5 33 183 20.33 9 21 33 24 5 33 1 4 2 2 0 9
+34 9 5 33 150 16.67 7 17 22 15 5 33 3 4 1 1 0 9
+35 9 13 33 217 24.11 21 24 29 8 13 33 1 4 1 3 0 9
+36 9 5 33 195 21.67 18 21 32 14 5 33 3 2 1 3 0 9
b
diff -r 18a51655db17 -r 21796f46e0ba test-data/fastx_nt_distribution.png
b
Binary file test-data/fastx_nt_distribution.png has changed
b
diff -r 18a51655db17 -r 21796f46e0ba test-data/fastx_nucleotides_distribution-in1.txt
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/fastx_nucleotides_distribution-in1.txt Tue May 08 13:27:48 2018 -0400
b
@@ -0,0 +1,37 @@
+column count min max sum mean Q1 med Q3 IQR lW rW A_Count C_Count G_Count T_Count N_Count Max_count
+1 9 23 34 288 32.00 33 33 33 0 33 33 3 1 4 1 0 9
+2 9 28 33 287 31.89 31 33 33 2 28 33 3 3 2 1 0 9
+3 9 13 34 268 29.78 28 33 33 5 21 34 5 1 0 3 0 9
+4 9 17 33 261 29.00 30 33 33 3 26 33 1 2 3 3 0 9
+5 9 22 33 269 29.89 30 33 33 3 26 33 3 3 3 0 0 9
+6 9 22 33 277 30.78 30 33 33 3 26 33 5 3 0 1 0 9
+7 9 21 33 258 28.67 24 33 33 9 21 33 4 1 3 1 0 9
+8 9 12 33 263 29.22 32 33 33 1 31 33 2 1 1 5 0 9
+9 9 29 33 290 32.22 33 33 33 0 33 33 3 3 2 1 0 9
+10 9 23 33 277 30.78 32 33 33 1 31 33 1 4 2 2 0 9
+11 9 12 33 245 27.22 21 31 33 12 12 33 5 2 1 1 0 9
+12 9 13 33 214 23.78 15 24 33 18 13 33 2 4 2 1 0 9
+13 9 5 33 249 27.67 29 31 33 4 23 33 2 1 1 5 0 9
+14 9 5 33 233 25.89 24 33 33 9 11 33 3 3 2 1 0 9
+15 9 15 33 251 27.89 24 33 33 9 15 33 5 1 1 2 0 9
+16 9 23 34 269 29.89 24 33 33 9 23 34 3 1 2 3 0 9
+17 9 13 34 266 29.56 33 33 33 0 33 33 2 3 1 3 0 9
+18 9 21 34 272 30.22 31 33 33 2 28 34 0 5 1 3 0 9
+19 9 5 34 244 27.11 27 30 33 6 18 34 4 4 1 0 0 9
+20 9 11 34 241 26.78 23 32 33 10 11 34 3 4 2 0 0 9
+21 9 13 33 240 26.67 24 27 33 9 13 33 1 4 0 4 0 9
+22 9 5 33 190 21.11 13 21 33 20 5 33 1 4 0 3 1 9
+23 9 5 33 205 22.78 16 26 33 17 5 33 4 4 1 0 0 9
+24 9 5 33 247 27.44 28 31 33 5 21 33 1 5 1 2 0 9
+25 9 11 34 241 26.78 24 33 33 9 11 34 3 4 0 2 0 9
+26 9 5 33 212 23.56 18 31 33 15 5 33 0 6 0 3 0 9
+27 9 5 33 227 25.22 21 26 33 12 5 33 3 4 1 1 0 9
+28 9 21 33 255 28.33 24 31 33 9 21 33 2 4 3 0 0 9
+29 9 5 33 228 25.33 21 30 33 12 5 33 2 4 1 2 0 9
+30 9 10 33 213 23.67 16 28 33 17 10 33 3 4 2 0 0 9
+31 9 5 33 236 26.22 21 31 33 12 5 33 1 4 1 3 0 9
+32 9 5 33 210 23.33 12 29 33 21 5 33 3 3 0 3 0 9
+33 9 5 33 183 20.33 9 21 33 24 5 33 1 4 2 2 0 9
+34 9 5 33 150 16.67 7 17 22 15 5 33 3 4 1 1 0 9
+35 9 13 33 217 24.11 21 24 29 8 13 33 1 4 1 3 0 9
+36 9 5 33 195 21.67 18 21 32 14 5 33 3 2 1 3 0 9
b
diff -r 18a51655db17 -r 21796f46e0ba test-data/fastx_nucleotides_distribution-out1.png
b
Binary file test-data/fastx_nucleotides_distribution-out1.png has changed
b
diff -r 18a51655db17 -r 21796f46e0ba tool_dependencies.xml
--- a/tool_dependencies.xml Wed Nov 11 12:39:18 2015 -0500
+++ /dev/null Thu Jan 01 00:00:00 1970 +0000
b
@@ -1,6 +0,0 @@
-<?xml version="1.0"?>
-<tool_dependency>
-    <package name="fastx_toolkit" version="0.0.13">
-        <repository changeset_revision="ec66ae4c269b" name="package_fastx_toolkit_0_0_13" owner="devteam" toolshed="https://toolshed.g2.bx.psu.edu" />
-    </package>
-</tool_dependency>