Previous changeset 1:606da4e1d925 (2013-08-19) Next changeset 3:72f03c2102ee (2013-08-21) |
Commit message:
Uploaded v0.0.2, updated tool versions to solve warning from core Galaxy filter tool being updated. No functional changes. |
modified:
README.rst secreted_protein_workflow.ga |
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diff -r 606da4e1d925 -r 3a0c0d1c388f README.rst --- a/README.rst Mon Aug 19 11:37:04 2013 -0400 +++ b/README.rst Wed Aug 21 11:58:54 2013 -0400 |
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@@ -4,12 +4,26 @@ It runs SignalP v3.0 (Bendtsen et al. 2004) and selects only proteins with a strong predicted signal peptide, and then runs TMHMM v2.0 (Krogh et al. 2001) on those, and selects only proteins without a predicted trans-membrane helix. -This workflow was used in Kikuchi et al (2001), and is a simplification of -the candidate effector protocol described in Jones et al (2009). +This workflow was used in Kikuchi et al. (2011), and is a simplification of +the candidate effector protocol described in Jones et al. (2009). See http://www.galaxyproject.org for information about the Galaxy Project. +Sample Data +=========== + +This workflow was developed and run on several nematode species. For example, +try the protein set for Bursaphelenchus xylophilus (Kikuchi et al. 2011): + +ftp://ftp.sanger.ac.uk/pub/pathogens/Bursaphelenchus/xylophilus/Assembly-v1.2/BUX.v1.2.genedb.protein.fa.gz + +You can upload this directly into Galaxy via this URL. Galaxy will handle +removing the gzip compression to give you the FASTA protein file which has +18,074 sequences. The expected result (selecting organism type Eukaryote) +is a FASTA protein file of 2,297 predicted secreted protein sequences. + + Citation ======== @@ -78,6 +92,19 @@ installation (SignalP v3.0 and TMHMM v2.0). +History +======= + +======= ====================================================================== +Version Changes +------- ---------------------------------------------------------------------- +v0.0.1 - Initial release to Tool Shed (May, 2013) + - Expanded README file to include example data +v0.0.2 - Updated versions of the tools used, inclulding core Galaxy Filter + tool to avoid warning about new ``header_lines`` parameter. +======= ====================================================================== + + Developers ========== |
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diff -r 606da4e1d925 -r 3a0c0d1c388f secreted_protein_workflow.ga --- a/secreted_protein_workflow.ga Mon Aug 19 11:37:04 2013 -0400 +++ b/secreted_protein_workflow.ga Wed Aug 21 11:58:54 2013 -0400 |
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@@ -62,8 +62,8 @@ }, "tool_errors": null, "tool_id": "signalp3", - "tool_state": "{\"organism\": \"{\\\"__class__\\\": \\\"RuntimeValue\\\"}\", \"fasta_file\": \"null\", \"chromInfo\": \"\\\"/opt/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\", \"truncate\": \"\\\"60\\\"\", \"__page__\": 0}", - "tool_version": "0.0.8", + "tool_state": "{\"__page__\": 0, \"truncate\": \"\\\"60\\\"\", \"chromInfo\": \"\\\"/opt/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\", \"fasta_file\": \"null\", \"organism\": \"{\\\"__class__\\\": \\\"RuntimeValue\\\"}\", \"__rerun_remap_job_id__\": null}", + "tool_version": "0.0.12", "type": "tool", "user_outputs": [] }, @@ -104,7 +104,7 @@ }, "tool_errors": null, "tool_id": "Filter1", - "tool_state": "{\"__page__\": 0, \"cond\": \"\\\"c14=='Y' or c15=='S'\\\"\", \"chromInfo\": \"\\\"/opt/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\", \"input\": \"null\"}", + "tool_state": "{\"__page__\": 0, \"__rerun_remap_job_id__\": null, \"cond\": \"\\\"c14=='Y' or c15=='S'\\\"\", \"input\": \"null\", \"header_lines\": \"\\\"0\\\"\", \"chromInfo\": \"\\\"/opt/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\"}", "tool_version": "1.1.0", "type": "tool", "user_outputs": [] @@ -152,8 +152,8 @@ }, "tool_errors": null, "tool_id": "seq_filter_by_id", - "tool_state": "{\"__page__\": 0, \"output_choice_cond\": \"{\\\"output_choice\\\": \\\"pos\\\", \\\"__current_case__\\\": 1}\", \"input_file\": \"null\", \"input_tabular\": \"null\", \"chromInfo\": \"\\\"/opt/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\", \"columns\": \"{\\\"__class__\\\": \\\"UnvalidatedValue\\\", \\\"value\\\": [\\\"1\\\"]}\"}", - "tool_version": "0.0.1", + "tool_state": "{\"__page__\": 0, \"output_choice_cond\": \"{\\\"output_choice\\\": \\\"pos\\\", \\\"__current_case__\\\": 1}\", \"input_file\": \"null\", \"__rerun_remap_job_id__\": null, \"input_tabular\": \"null\", \"chromInfo\": \"\\\"/opt/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\", \"columns\": \"{\\\"__class__\\\": \\\"UnvalidatedValue\\\", \\\"value\\\": [\\\"1\\\"]}\"}", + "tool_version": "0.0.5", "type": "tool", "user_outputs": [] }, @@ -187,8 +187,8 @@ }, "tool_errors": null, "tool_id": "tmhmm2", - "tool_state": "{\"__page__\": 0, \"fasta_file\": \"null\", \"chromInfo\": \"\\\"/opt/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\"}", - "tool_version": "0.0.7", + "tool_state": "{\"__page__\": 0, \"fasta_file\": \"null\", \"chromInfo\": \"\\\"/opt/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\", \"__rerun_remap_job_id__\": null}", + "tool_version": "0.0.11", "type": "tool", "user_outputs": [] }, @@ -229,7 +229,7 @@ }, "tool_errors": null, "tool_id": "Filter1", - "tool_state": "{\"__page__\": 0, \"cond\": \"\\\"c5== 0\\\"\", \"chromInfo\": \"\\\"/opt/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\", \"input\": \"null\"}", + "tool_state": "{\"__page__\": 0, \"__rerun_remap_job_id__\": null, \"cond\": \"\\\"c5== 0\\\"\", \"input\": \"null\", \"header_lines\": \"\\\"0\\\"\", \"chromInfo\": \"\\\"/opt/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\"}", "tool_version": "1.1.0", "type": "tool", "user_outputs": [] @@ -279,8 +279,8 @@ }, "tool_errors": null, "tool_id": "seq_filter_by_id", - "tool_state": "{\"__page__\": 0, \"output_choice_cond\": \"{\\\"output_choice\\\": \\\"pos\\\", \\\"__current_case__\\\": 1}\", \"input_file\": \"null\", \"input_tabular\": \"null\", \"chromInfo\": \"\\\"/opt/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\", \"columns\": \"{\\\"__class__\\\": \\\"UnvalidatedValue\\\", \\\"value\\\": [\\\"1\\\"]}\"}", - "tool_version": "0.0.1", + "tool_state": "{\"__page__\": 0, \"output_choice_cond\": \"{\\\"output_choice\\\": \\\"pos\\\", \\\"__current_case__\\\": 1}\", \"input_file\": \"null\", \"__rerun_remap_job_id__\": null, \"input_tabular\": \"null\", \"chromInfo\": \"\\\"/opt/galaxy-dist/tool-data/shared/ucsc/chrom/?.len\\\"\", \"columns\": \"{\\\"__class__\\\": \\\"UnvalidatedValue\\\", \\\"value\\\": [\\\"1\\\"]}\"}", + "tool_version": "0.0.5", "type": "tool", "user_outputs": [] } |