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APOSTL_Static_Bubblegraph_Generator.xml |
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| diff -r 66faf09e5e8e -r 412e18203eb5 APOSTL_Static_Bubblegraph_Generator.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/APOSTL_Static_Bubblegraph_Generator.xml Tue Mar 15 15:19:13 2016 -0400 |
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| @@ -0,0 +1,131 @@ +<tool id="APOSTL_Static_Bubblegraph_Generator" name="APOSTL Static Bubblegraph Generator"> + <description></description> + <command interpreter="python">APOSTL_Static_Bubblegraph_Generator.py $list_file $prey_file $crapome $color $label $cutoff $type $inc_file $exc_file $outfile $NSAF_zoom $SAINT_zoom $SAINT $NSAF \$INSTALL_RUN_PATH/</command> + <requirements> + <requirement type="set_environment">INSTALL_RUN_PATH</requirement> + </requirements> + <inputs> + <param type="select" name="type" label="MaxQuant or Scaffold"> + <option value="MQ">MaxQuant</option> + <option value="SC">Scaffold</option> + </param> + <param format="txt" name="list_file" type="data" label="List File"/> + <param format="txt" name="prey_file" type="data" label="Prey File"/> + <param format="txt" name="crapome" type="data" label="Crapome File" optional="true"/> + <param name="color" type="select" label="Color" optional="true"> + <option value="crapome">Crapome</option> + <option value="red">Red</option> + <option value="blue">Blue</option> + </param> + <param type="boolean" name="label" checked="true" label="Use Labels"/> + <param type="float" name="cutoff" label="SAINT Score Cutoff" help="Select a value between 0 and 1 written like 0.8." value="0.8"/> + <param format="txt" name="inc_file" type="data" label="List of Uniprot IDs to Include" optional="true"/> + <param format="txt" name="exc_file" type="data" label="List of Uniprot IDs to Exclude" optional="true"/> + </inputs> + <outputs> + <data format="txt" name="outfile" label="Outfile"/> + <data format="png" name="NSAF_zoom" label="Zoom NSAF" /> + <data format="png" name="SAINT_zoom" label="Zoom SAINT" /> + <data format="png" name="NSAF" label="NSAF" /> + <data format="png" name="SAINT" label="SAINT" /> + </outputs> + <stdio> + <regex match="error" + source="stdout" + level="fatal" + description="Unknown error"/> + </stdio> + + <tests> + <test> + <param name="input" value="fa_gc_content_input.fa"/> + <output name="out_file1" file="fa_gc_content_output.txt"/> + </test> + </tests> + <help> +Post-processing: +Once SAINTexpress has been run, APOSTL is able to read the resulting "list.txt" file. Fromhere APOSTL does a number of things: + +APOSTL calculates NSAF values for each prey based on the average spectra observed for each bait + +OPTIONAL: APOSTL calculates the probability of a specific interaction based on prey revalence in the CRAPome + +Bubble graphs are generated for each bait where: + +- x axis is the natural log of the NSAF values + +- y axis is the observed log2 fold change (as compared to control) + +- bubble radius is proportional to the average observed spectra + +OPTIONAL: bubble color corresponds to the CRAPome probability of a specific interaction in which an 80% cutoff is applied where prey with less than 80% are colored tan + +APOSTL queries ConsensusPathDB for protein-protein interactions within your data and then formats the resulting network for simple cytoscape import using the "import network from file" option. + +INPUTS: + +List File: + +- SAINTexpress generated "list.txt" file + +Prey File: + +- SAINT pre-processing generated "prey.txt" file used to run SAINTexpress + +Crapome File: + +- raw output from Crapome Workflow 1 query (http://www.crapome.org) + +Color: + +- Specify the color of the bubbles within the graph. + +- If "crapome" is chosen and 'crapome' file is specified, bubbles will be color based on crapome specificity + +Use labels: + +- Adds gene name labels to bubbles within the "zoomed in" graphs +SAINT Score Cutoff: + +- Choose Saintscore cutoff (between 0-1) to be used for filtering the "zoomed in" graphs (default = 0.8) + +- Also used for filtering during the generation of the cytoscape network + +Species: + +- Human, mouse, or yeast + +Interaction Confidence: + +- Interaction confidence value (0-1) used to filter the interactions with the ConsensusPathDB database + +- Suggestions: + +* low: 0 + +* medium: 0.5 + +* high: 0.7 + +* very high: 0.9 + +OUTPUTS: + +Bubble Graphs: + +- Unfiltered Data: + +* NSAF v. Log2(FoldChange) + +* SAINTscore v. Log2(FoldChange) + +- Filtered by SAINT Score + +* NSAF v. Log2(FoldChange) + +* SAINTscore v. Log2(FoldChange) + +Output.txt +- SAINTexpress "list.txt" output with additional columns that were used during the analysis + </help> +</tool> |