| Next changeset 1:5d05b34a5fdf (2014-05-28) |
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added:
otu_clustering/README.txt otu_clustering/cluster_otu.xml otu_clustering/test-data/seqs_derep.fasta otu_clustering/test-data/seqs_otus.fasta |
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| diff -r 000000000000 -r 52043d3d71f5 otu_clustering/README.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/otu_clustering/README.txt Wed May 28 21:22:08 2014 -0400 |
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| @@ -0,0 +1,56 @@ +Galaxy wrappers for USEARCH - UPARSE (OTU Clustering) +===================================================== + +USEARCH requires a licence. Therefore an automated installation is not +possible at the moment. + +Requirements +===================================================== +Get your licenced USEARCH version 7, but not later then 7.0.1002 from here: +http://www.drive5.com/usearch/download.html + + +Manual Installation Steps +===================================================== +USEARCH is distributed as one file, known as the binary file or executable +file. It is completely self-contained: it does not require configuration +files, environment variables, third-party libraries or other external +dependencies. There is no setup script or installer because they're not +needed. To install it, all you do is download or copy the binary to a +directory that is accessible from the computer where you want to run the code. + +Step1: +Rename the binary file to usearch. + +Step2: +Move the binary file (usearch) to /usr/local/bin +Ensure /usr/local/bin is in your path. If needed add /usr/local/bin/ to your +path. + +Step3: +Ensure that you have read and execute permissions for the binary file. +If needed, use the chmod command to set the execute bit, e.g.: +chmod +x /usr/local/bin/usearch + + +Further installation information and help can be found at: +http://drive5.com/usearch/manual/install.html + + +Disclaimer +===================================================== +This source code is provided by QFAB "as is", in the hope that it will be +useful, and any express or implied warranties, including, but not limited to, +the implied warranties of merchantability and fitness for a particular purpose +are disclaimed. +IN NO EVENT SHALL QFAB BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, +SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY +OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT(INCLUDING +NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOURCE +CODE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. + +License +===================================================== +This work by QFAB Bioinformatics (as part of the GVL project http://genome.edu.au) +is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 +International License. |
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| diff -r 000000000000 -r 52043d3d71f5 otu_clustering/cluster_otu.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/otu_clustering/cluster_otu.xml Wed May 28 21:22:08 2014 -0400 |
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| @@ -0,0 +1,81 @@ +<tool id="cluster_otu" name="Cluster OTU" version="1.0.0"> + <description>Cluster OTUs</description> + <command> + usearch -cluster_otus $input -otus $output -otuid $radius 2>1 + </command> + +<inputs> + <param name="input" type="data" format="fasta" label="Input sequence file" + help="Size annotation is required, see description below!"/> + <param name="radius" type='float' value='0.97' label='Minimum cluster radius' + help="default=0.97, corresponds to minimum of 97% sequence identity" /> +</inputs> + +<outputs> + <data name='output' format="fasta" /> +</outputs> + +<help> +=========== +Description +=========== + +Performs OTU clustering using the USEARCH-Tool-Suite_. + +.. _USEARCH-Tool-Suite: http://www.drive5.com/usearch/ + +----- + +----- +Input +----- + +FASTA file with size annotation in the input line, e.g. >sequenceXXXX;size=1443; + +.. class:: warningmark + +Size annotation is required. + +---------- +Parameters +---------- + +radius + (Usearch v7.0.1002 and earlier) specifies the OTU 'radius' as the minimum identity between an OTU member sequence and the representative sequence as a fractional identity 0.0 to 1.0. Default is 0.97, which corresponds to a minimum identity of 97%. + +------ +Output +------ + +FASTA output of the representative sequences. + +.. class:: warningmark + +If there is a "size=XX" annotation in the identifier line of the output file, please note this is NOT the number of sequences in this cluster. The size annotation is then coming from the input file. + +----- + +========= +Resources +========= + +UPARSE_ (OTU Clustering) + +.._UPARSE: http://drive5.com/usearch/manual/cluster_otus.html + +**Author** + +Robert C. Edgar (bob@drive5.com) + +**Wrapper Author** + +QFAB Bioinformatics (support@qfab.org) +</help> +<tests> + <test> + <param name="input" value="seqs_derep.fasta" /> + <param name="radius" value="0.97" /> + <output name="output" file="seqs_otus.fasta" ftype="fasta" lines_diff="10" /> + </test> +</tests> +</tool> |
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| diff -r 000000000000 -r 52043d3d71f5 otu_clustering/test-data/seqs_derep.fasta --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/otu_clustering/test-data/seqs_derep.fasta Wed May 28 21:22:08 2014 -0400 |
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| b'@@ -0,0 +1,28685 @@\n+>258155;size=2;\n+AGAGTTTGATCATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGATGAAGCACCTTCGGGTGT\n+GAATTAGCGGCGAACGGGTGAGGAACACGTGAGAAATCTGCCTTCAACACTGGGATAACTCCGGGAAACCGGGGCTAATA\n+CCGGATATGAAATCTGCGGGCATCCGCGGATTTGGAAAGTTTTTCGGTTGAAGATGATCTCGCGGCCTATCAGCTTGTTG\n+GTGAGGTAATGGCTCACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCC\n+CAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGACCCAGCGACGCCGCGTGCGGGATGA\n+AGGCCTTCGGGTTGTAAACCGCTTTCAGCAGGGAAGAAGCGAAAGTGACGGTACCTGCAGAAGAAGCACCGGCTAACTAT\n+GTGCCAGCAGCCGCGGTAATACATAGGGTGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGTGGTTCGTC\n+ACGTCGGATGTGAAACTCTGGGGCTTAACCCCAGACCTGCATTCGATACGGGCGAGCTTGAGTATGGTAGGGGAGTCTTG\n+AATTCCTGGTGTAGCGGTGGAATGCGCAGATATCAGGAGGAACACCAATGGCGAAGGCAGGACTCTGGGCCATTACTGAC\n+ACTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGGTGGGCACTAGTTGTG\n+GGGACCTTCCACGGTCTCTGCGACGCAGCTAACGCATTAAGTGCCCCGCCTGGGGAGTACGATCGCAAGATTAAAACTCA\n+AAGGAATTGACGGGGCCCCGCACAAGCAGCGGAGCATGCGGCTTAATTCGACGCAACGCGAAGAACCTTACCAAGGCTTG\n+ACATATACAGGAATATGGCAGAGATGTCATAGCCGCAAGGTCTGTATACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTC\n+GTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCGTTCTGTGTTGCCAGCATTTAGTTGGGGACTCACAGGAGA\n+CTGCCGGGGTTAACTCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGTCTTGGGCTGCACGCATGCTAC\n+AATGGCTGGTACAAACGGCTGCAATACCGCAAGGTGGAGCGAATCCGAGAAAGCCAGTCTCAGTTCGGATTGGGGTCTGC\n+AACTCGACCCCATGAAGTCGGAGTTGCTAGTAATCATAGATCAGCAACGCTACGGTGAATACGTTCCCGGGGCTTGTACA\n+CACCGCCCGTCACGTCACGAGAGTCGGTAACACCCGAAGTCAGTGGCCCAACCGCAAGGAGGGAGCTGCCGAAGGTGGGA\n+TCGGTGATTGGGACGAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATCACCTCCTTT\n+>248442;size=2;\n+AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGCGAAATCTCCTTCGGGAGT\n+GAGTAGAGCGGCGGACGGGTGAGTAACGCGTAGGAATCTACCCAGTGGTGGGGGACAACCTGGGGAAACCCAGGCTAATA\n+CCGCATACGCCCTACGGGGGAAAGCGGGGGCTCTCTTCGGAGACCTCGCGCCATTGGATGAGCCTGCGTTGGATTAGCTA\n+GTTGGTGGGGTAAAGGCCTACCAAGGCGACGATCCATAGCTGGTCTGAGAGGACGATCAGCCACACTGGGACTGAGACAC\n+GGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCCATGCCGCGTGTGTG\n+AAGAAGGCTCTAGGGTTGTAAAGCACTTTCAGTAGGGAGAAAAAGCTCATGTTTAATAGATGTGAGTGTTGATGTTACCT\n+ACAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG\n+TAAAGCGCGCGTAGGCGGTTTGTTAAGTCGGATGTGAAAGCCCCGGGCTCAACCTGGGAATTGCATTCGATACTGATCGA\n+CTAGAGTACGAGAGAGGGAGGTAGAATTCCACGTGTAGCGGTGAAATGCGTAGATATGTGGAGGAATACCGGTGGCGAAG\n+GCGGCCTCCTGGCTCGATACTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGC\n+CGTAAACGATGTCAACTAGCCGTTGGGGAACTTGATTCCTTAGTGGCGCAGCTAACGCAATAAGTTGACCGCCTGGGGAG\n+TACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAAC\n+GCGAAGAACCTTACCAGGTCTTGACATCCTGAAAACTTTCCAGAGATGGATTGGTGCCTTCGGGAATTCAGTGACAGGTG\n+CTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGTAACGAGCGCAACCCTTGTCCTTAGTTGCCA\n+GCACGTAATGGTGGGAACTCTAAGGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGACGACGTCAAGTCATCATGGCC\n+CTTACGACCTGGGCTACACACGTGCTACAATGGTCGGTACAGACGGCTGCGAACCCGCGAGGGGGAGCGAATCCGAGAAA\n+ACCGATCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGCGAATCAGAATGTCGC\n+GGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCTCCAGAAGTGGTTAGCCTAACC\n+TTAGGGAGGGCGATCACCACGGAGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACC\n+>229462;size=1;\n+AGAGTTTGATCATGGCTCAGGATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGTAACAGGGATTGCTTGCA\n+ATCCGCTGACGACCGGCGCACGGGTGCGTAACGCGTATGCAACTTACCTTTTACTGGGGGATAGTCAAGAGAAATTTTGA\n+ATAATACCCCATACGATCTAACTCACTCCTGTGAGATAGAAGAAAATTTCGATGGTAAAAGATAGGCATGCGTCCTATTA\n+GTTTGTTGGTGAGGTAACGGCTTACCAAGACTACGATAGGTAGGGGTCCCGAGAGGGAGATCCCCCACACTGGTACTGAG\n+ACACGGACCAGACTCCTACGGGAGGCAGCAGTGAGGAATATTGGACAATGGAGGCAACTCTGATCCAGCCATGCCGCGTG\n+CAGGAAGACAGCCCTATGGGTTGTAAACTGCTTTTATACAGGAAGAAACGTTAGTACGTGTACTAGCCTGACGGTACTGT\n+AAGAATAAGGATCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGATCCAAGCGTTATCCGGAATCATTGGGTTT\n+AAAGGGTCCGCAGGCGGTCTATTAAGTCAGAGGTGAAATCCTATCGCTCAACGATAGAACTGCCTTTGATACTGCTAGAC\n+TTGAGTTATTGTGAAGTAGTTAGAATGTGTAGTGTAGCGGTGAAATGCATAGATATTACACAGAATACCGATTGCGAAGG\n+CAGATTACTAACAATACACTGACGCTCAGGGACGAAAGCGTGGGTAGCGAACAGGATTAGATACCCTGGTAGTCCACGCC\n+GTAAACGATGGTCACTAGCTGTTT'..b'AATTACTGGGCTTAAAGAGCTCGTAGGTGGTTAAAAAAGTTGATGGTGAAATCCCAAGGCTCAAC\n+CTTGGAACTGCCATCAAAACTTTTTAGCTAGAGTGTGATAGAGGAAAGTGGAATTTCTAGTGTAGAGGTGAAATTCGTAG\n+ATATTAGAAAGAACACCAAATGCGAAGGCAACTTTCTGGGTCACTACTGACACTGAGGAGCGAAAGCATGGGTAGCGAAG\n+AGGATTAGATACCCTCGTAGTCCATGCCGTAAACGATGTGTGCTAGACGTTGGAAATATATTTTTCAGTGTCGCAGCGAA\n+AGCATTAAGCACACCGCCTGGGGAGTACGACCGCAAGGTTAAAACTCAAATGAATTGACGGGGACCCGCACAAGCAGTGG\n+AGCATGTGGTTTAATTCGAAGATACGCGCAGAACCTTACCAACACTTGACATGTTCGTCGCGAGACCAAGAGATTGGTTT\n+CTTCATTTAGTTGGACGAAACACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAA\n+CGAGCGCAACCCCTACTTTTAGTTGCCACCATTTAGTTGGGCACTTTAAAAGAACTGCCAGTGATAAGCTGGAGGAAGGT\n+GGGGATGACGTCAAGTCCTCATGGCCCTTACGTGTTGGGCTACACACGTGCTACAATGGCACTTACAATGGGAAGCAAAG\n+AGGTGACTCCTAGCTAATCCCAAAAATGTGTCTCAGTTCGGATTGCACTCTGCAACTCGAGTGCATGAAGCTGGAATTGC\n+TAGTAATCGCGAATCAGCGCGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGAAGTTGGT\n+TACACCTTAAGGCAAATCGTCAACCTTCGGGAGACATTTGACTACGGTACGATCAGCAACTGGGGT\n+>356409;size=1;\n+AGGGTTTGATCATGGCTCAGATTGAACGCTGGCGGTAGGCTTAACACATGCAAGTCGTGCGAGAAAGTACCTTCGGGTGC\n+GAGTAGAGCGGCGGACGGGTGAGTAACGCGTAGGAATCTACCTAGTAGAAGGGGATAGCCCGGGGAAACTCGGATTAATA\n+CCGTATACCTCCTTAGGGAGAAAGAGGGCTTAGCTTTGATGCTCTCGCTATTAGATGAGCCTGCGTAAGATTAGCTTGTT\n+GGTGAGGTAATGGCTCACCAAGGCGACGATCTTTAGCTGGTCTGAGAGGACGATCAGCCACATTGGGACTGAGACACGGC\n+CCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATACCGCGTGTGTGAAG\n+AAGGCCTTCGGGTTGTAAAGCACTTTAAGTAAGGAGGAAAAGATTGTAGTTAATACCTGCAATCCGTGACGTTACTTACA\n+GAATAAGGACCGGCTAATTCCGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAAGCGTTAATCGGAATTACTGGGCGTAA\n+AGCGCGCGTAGGTGGTTTTTTAAGTTGGATGTGAAAGCCCTGGGCTCAACCTAGGAACTGCATCCAAAACTAGATGACTA\n+GAGTACGAAAGAGGGAAGTAGAATTCACAGTGTAGCGGTGGAATGCGTAGATATTGTGAAGAATACCAATGGCGAAGGCA\n+GCTTCCTGGTTCTGTACTGACACTGAGGTGCGAAAGCGTGGGTAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGT\n+AAACGATGACAACTAGCTGTTGGGAGACAAGATCTCTCAGTGGCGCAGCTAACGCTTTAAGTTGTCCGCCTGGGGAGTAC\n+GGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCG\n+AAAAACCTTACCTACTCTTGACATACTTGGAAGCTCTTGTAATGAGAGTGTGCTTTTAGAGCCAAGATACAGGTGCTGCA\n+TGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCATAACGAGCGCAACCCTTACCCTTATTTGCCAGCGGT\n+TCGGCCGGGAACTATAAGGGGACTGCCGGTGACAAACCGGAGGAAGGTGAGGACGACGTCAAGTCATCATGGCCCTTACG\n+AGTAGGGCTACACACGTGCTACAATGGGGAATACAGACGGACGCTAAAGCGTGAGCTGGTGCTAATCCTAAAAAATTTCT\n+CGTAGTCCGGATTGCAGTCTGCAACTCGACTGCATGAAGTCGGAATCGCTAGTAATCGCGAATCAGCATGTCGCGGTGAA\n+TACGTTCTCGGGTCTTGTACACACCGCCCGTCACACCATGGAAGTGTGTTGCACCAGAAGTAGGTAGTCTAACCTTCGGG\n+AAGGCGCTTACCACGGTGTGATCCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTA\n+>354371;size=1;\n+AGAGTTTGATCATGGCTCAGAATGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGAGATCTTCGGATCTAGTGG\n+CAGACGGGTGAGTAACGCGTGGGAACCTGCCCAGTAGTAGAGAATAACTTGGGGAAACTTAAGCTAATACTTTATACGTC\n+CTTCGGGAGAAAGCTTTATGCGCTATTGGATGGGCCCGCGTTAGATTAGTTTGTTGGTGAGGTAACGGCTCACCAAGGCG\n+ACGATCTATAGCTGGTCTGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGT\n+GGGGAATATTGGACAATGGGGGCAACCCTGATCCAGCGATGCCGCGTGAGTGATGAAGGCCCTAGGGTTGTAAAACTCTT\n+TCGTCAGGGAAGATAATGACGGTACCTGAAGAAGAAGATCCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGG\n+TCTAGCGTTATTCGGAATTACTGGGCTTAAAGAGTTCGTAGGTGGTTGAAAAAGTTGGTGGTGAAATCCCAGAGCTTAAC\n+TCTGGAACTGCCATCAAAACTTTTCAGCTAGAGTTTGATAGAGGAAAGCAGAATTTCTAGTGTAGAGGTGAAATTCGTAG\n+ATATTAGAAAGAATACCAATTGCGAAGGCAGCTTTCTGGATCATTACTGACACTGAGGAACGAAAGCATGGGTAGCGAAG\n+AGGATTAGATACCCTCGTAGTCCATGCCGTAAACGATGTGTGTTAGACGTTGGAAATTTATTTTCAGTGTCGCAGCGAAA\n+GCGATAAACACACCGCCTGGGGAGTACGACCGCAAGGTTAAAACTCAAATGAATTGACGGGGACCCGCACAAGTAGTGGA\n+GCATGTGGTTTAATTCGAAGATACGCGCAGAACCTTACCAACACTTGACATGTTCGTCGCGACTTTAAGAGATTAAAGTT\n+TTCGGTTCGGCCGGACGAAACACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAA\n+CGAGCGCAACCCTCACTTTTAGTTGCCATCATTTAGTTGGGCACTCTGAAAGAACTGCCAGTGATAAGCTGGAGGAAGGT\n+GGGGATGACGTCAAGTCCTCATGGCCCTTACGTGTTGGGCTACACACGTGCTACAATGGTATCTACAACAGGAAGCAAAA\n+CAGCGATGTTAAGCAAATCCTTAAAAGATACCTCAGTTCGGATTGCACTCTGCAACTCGAGTGCATGAAGCTGGAATTAC\n+TAGTAATCGTGGATCAGCGTGCCACGGTGAATGCGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTTGGT\n+TCTACCTTAAGGCAAGGTTTAATACCCTTGACCACGGTATAGTCAGCGACTGGGGTGAAGTCATAACAAGGTAGCCGTA\n' 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| diff -r 000000000000 -r 52043d3d71f5 otu_clustering/test-data/seqs_otus.fasta --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/otu_clustering/test-data/seqs_otus.fasta Wed May 28 21:22:08 2014 -0400 |
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| b'@@ -0,0 +1,11017 @@\n+>258155;size=2;\n+AGAGTTTGATCATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGATGAAGCACCTTCGGGTGT\n+GAATTAGCGGCGAACGGGTGAGGAACACGTGAGAAATCTGCCTTCAACACTGGGATAACTCCGGGAAACCGGGGCTAATA\n+CCGGATATGAAATCTGCGGGCATCCGCGGATTTGGAAAGTTtttCGGTTGAAGATGATCTCGCGGCCTATCAGCTTGTTG\n+GTGAGGTAATGGCTCACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCC\n+CAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGACCCAGCGACGCCGCGTGCGGGATGA\n+AGGCCTTCGGGTTGTAAACCGCTTTCAGCAGGGAAGAAGCGAAAGTGACGGTACCTGCAGAAGAAGCACCGGCTAACTAT\n+GTGCCAGCAGCCGCGGTAATACATAGGGTGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGTGGTTCGTC\n+ACGTCGGATGTGAAACTCTGGGGCTTAACCCCAGACCTGCATTCGATACGGGCGAGCTTGAGTATGGTAGGGGAGTCTTG\n+AATTCCTGGTGTAGCGGTGGAATGCGCAGATATCAGGAGGAACACCAATGGCGAAGGCAGGACTCTGGGCCATTACTGAC\n+ACTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGGTGGGCACTAGTTGTG\n+GGGACCTTCCACGGTCTCTGCGACGCAGCTAACGCATTAAGTGCCCCGCCTGGGGAGTACGATCGCAAGATTAAAACTCA\n+AAGGAATTGACGGGGCCCCGCACAAGCAGCGGAGCATGCGGCTTAATTCGACGCAACGCGAAGAACCTTACCAAGGCTTG\n+ACATATACAGGAATATGGCAGAGATGTCATAGCCGCAAGGTCTGTATACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTC\n+GTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCGTTCTGTGTTGCCAGCATTTAGTTGGGGACTCACAGGAGA\n+CTGCCGGGGTTAACTCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGTCTTGGGCTGCACGCATGCTAC\n+AATGGCTGGTACAAACGGCTGCAATACCGCAAGGTGGAGCGAATCCGAGAAAGCCAGTCTCAGTTCGGATTGGGGTCTGC\n+AACTCGACCCCATGAAGTCGGAGTTGCTAGTAATCATAGATCAGCAACGCTACGGTGAATACGTTCCCGGGGCTTGTACa\n+cacCGCCCGTCACGTCACGAGAGTCGGTAACACCCGAAGTCAGTGGCCCAACCGCAAGGAGGGAGCTGCCGAAGGTGGGA\n+TCGGTGATTGGGACGAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATCACCTCCTTT\n+>248442;size=2;\n+AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGCGAAATCTCCTTCGGGAGT\n+GAGTAGAGCGGCGGACGGGTGAGTAACGCGTAGGAATCTACCCAGTGGTGGgggACAACCTGGGGAAACCCAGGCTAATA\n+CCGCATACGCCCTACGGgggAAAGCGGgggCTctctTCGGAGACCTCGCGCCATTGGATGAGCCTGCGTTGGATTAGCTA\n+GTTGGTGGGGTAAAGGCCTACCAAGGCGACGATCCATAGCTGGTCTGAGAGGACGATCAGCCACACTGGGACTGAGACAC\n+GGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGgggAAACCCTGACGCAGCCATGCCGCGTgtgtg\n+AAGAAGGCTCTAGGGTTGTAAAGCACTTTCAGTAGGGAGAAaaaGCTCATGTTTAATAGATGTGAGTGTTGATGTTACCT\n+ACAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG\n+TAAAGCgcgcgTAGGCGGTTTGTTAAGTCGGATGTGAAAGCCCCGGGCTCAACCTGGGAATTGCATTCGATACTGATCGA\n+CTAGAGTACGAgagagGGAGGTAGAATTCCACGTGTAGCGGTGAAATGCGTAGATATGTGGAGGAATACCGGTGGCGAAG\n+GCGGCCTCCTGGCTCGATACTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGC\n+CGTAAACGATGTCAACTAGCCGTTGGGGAACTTGATTCCTTAGTGGCGCAGCTAACGCAATAAGTTGACCGCCTGGGGAG\n+TACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGgggCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAAC\n+GCGAAGAACCTTACCAGGTCTTGACATCCTGAAAACTTTCCAGAGATGGATTGGTGCCTTCGGGAATTCAGTGACAGGTG\n+CTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGTAACGAGCGCAACCCTTGTCCTTAGTTGCCA\n+GCACGTAATGGTGGGAACTCTAAGGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGACGACGTCAAGTCATCATGGCC\n+CTTACGACCTGGGCTACacacGTGCTACAATGGTCGGTACAGACGGCTGCGAACCCGCGAGGgggAGCGAATCCGAGAAA\n+ACCGATCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGCGAATCAGAATGTCGC\n+GGTGAATACGTTCCCGGGCCTTGTACacacCGCCCGTCACACCATGGGAGTGGGTTGCTCCAGAAGTGGTTAGCCTAACC\n+TTAGGGAGGGCGATCACCACGGAGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACC\n+>229462;size=1;\n+AGAGTTTGATCATGGCTCAGGATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGTAACAGGGATTGCTTGCA\n+ATCCGCTGACGACCGGCGCACGGGTGCGTAACGCGTATGCAACTTACCTTTTACTGGgggATAGTCAAGAGAAATTTTGA\n+ATAATACCCCATACGATCTAACTCACTCCTGTGAGATAGAAGAAAATTTCGATGGTAAAAGATAGGCATGCGTCCTATTA\n+GTTTGTTGGTGAGGTAACGGCTTACCAAGACTACGATAGGTAGGGGTCCCGAGAGGGAGATCCcccACACTGGTACTGAG\n+ACACGGACCAGACTCCTACGGGAGGCAGCAGTGAGGAATATTGGACAATGGAGGCAACTCTGATCCAGCCATGCCGCGTG\n+CAGGAAGACAGCCCTATGGGTTGTAAACTGCTTTTATACAGGAAGAAACGTTAGTACGTGTACTAGCCTGACGGTACTGT\n+AAGAATAAGGATCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGATCCAAGCGTTATCCGGAATCATTGGGTTT\n+AAAGGGTCCGCAGGCGGTCTATTAAGTCAGAGGTGAAATCCTATCGCTCAACGATAGAACTGCCTTTGATACTGCTAGAC\n+TTGAGTTATTGTGAAGTAGTTAGAATGTGTAGTGTAGCGGTGAAATGCATAGATATTACACAGAATACCGATTGCGAAGG\n+CAGATTACTAACAATACACTGACGCTCAGGGACGAAAGCGTGGGTAGCGAACAGGATTAGATACCCTGGTAGTCCACGCC\n+GTAAACGATGGTCACTAGCTGTTT'..b'ACTCCGTG\n+CCAGCAGCCGCGGTAATACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTAGGCGGGTCTTTAAG\n+TCAGTGGTGAAAGCCTGCAGCTTAACTGTAGAATTGCCATTGATACTGAAGGCCTTGAGTGTAGTAGAAGTAGGCGGAAT\n+ATGGCATGTAGCGGTGAAATGCATAGATATGCCATAGAACACCGATTGCGAAGGCAGCTTACTATGTTACAACTGACGCT\n+GAGGGACGAAAGCGTGGGTAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATTACTCGCTGTTAGC\n+GATATACAGTTAGCGGCCAAGCGAAAGCATTAAGTAATCCACCTGGGGAGTACGCCGGCAACGGTGAAACTCAAAGGAAT\n+TGACGGgggCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGATACGCGAGGAACCTTACCAGGGTTTAAATGGAA\n+ATTGACAGACGCCGAAAGGTGTCCTTCTTCGGACAATTTTCAAGGTGCTGCATGGTTGTCGTCAGCTCGTGCCGTGAGGT\n+GTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTACTGTTAGTTGCCAGCGGATAACGCCGGGGACTCTAGCAGAACTGCC\n+AGCGCAAGCTGAGAGGAAGGTGGGGACGACGTCAAATCATCACGGGCCTTACATCCTGGGCCAcacacGTGCTACAATGG\n+TAGTGACAGANNnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn\n+nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn\n+nnnnnnnnnnnnnnnnnnTGGGAGTGCCTGAAGTCGGTAACCGAAAGGAGCTGCCTAGGGTAAAACTAGTAACTAGGGCT\n+AAGTCGTAACAAGGTAGCCGTACCGGAAGGTGTGGCTGGAACACCTCCTT\n+>103462;size=1;\n+GATGAACGCTAGCGGTGTACTTTAGGTATGCAAGTCGAACGGTCCGGGTTTACTCGGACAGTGGCAGACGGGTGAGTAAC\n+GCGTTGGTACTCGCCCTCTGGTGTGGAATAGCCTGCTGAAAGGTGGGGTAATACCGCATAAGTCCGACCTAGTCGGATAA\n+AGGGCATAACTGCCCGCCGGAGGAGAGGCCTGCGTCCTATCAGCTTGTTGGTGAGGTAATGGCTCACCAAGGCGATGACG\n+GGTAGCTGGTGTGAGAGCACGATCAGCCACAGGGTCACTGAGACACGGGGCCCACACCTACGGGTGGCAGCAACCAGGAA\n+TATTGCGCAATGGGCGAAAGCCTGACGCAGTGACACCGCGTGAAGGATGAAGGCTTTCGGGTCGTAAACTTCTTTTCTAA\n+AGGAAGATAATGACGGTACTTTAGGAATAAGCACCTGCAAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGC\n+GTTATCCGGATTTACTGGGCGTAAAGAGTTTCGTAGGCGTCTTTCTGCATCTTGTTTTAAATCCCAAGGCTTAACCTTGG\n+AAAAGAACAAGAGATGGGTAAGATAGAACTAGGTTGGgggTGCTGGAACTCCCGGTGGAGGGGTGAAATCCGTTGATATC\n+GGGAAGAACACCAAGGGCGAAGGCAGGCACCTAGGCCTTAGTTGACGCTGATGAACGAAAGCTAGGGTAGCAAAAGGGAT\n+TAGAGACCCCTGTAGTCCTAGCCGTAAACGATCCCTACTAGTTGCCCCGATTTATATTGGGGTGGCGTAAGCTAACGCGT\n+TAAGTAGGGCGCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAAGGAATTGACGGGGATCTGCACAACCAGGGGAGCAT\n+GTGGTTCAATTCGATACGAAGCGTAGGACCTTACCTGGGCTTGACATGCTACTGCATACTTTGGGAAACCAGAGTAGCCT\n+TAGAGGGTGTAGCACAGCTGCTGCATGGCTGTCGTCAGCTCTAGTCGTGAGATGTTCCCTTAAGTGGGGTATAGAGCGCA\n+ACCCTTACTCCGTGTTAtatatCACGGAGAACTGCCTCAGTTTTACTGGGGAGGAAGGAGAGGATGACGTCAAGTCAGCA\n+CGGCGCTTACGTCCAGGGCGACacacaTCCTACAATCCCTACTACAACGAGTTGCGAAAGAGTAATCTGGAGCTAATCTC\n+TTAAAAGTAGGGTCAGTTCAGATTGTGGGCTGAAACCCGCCCACATGAAGCCGGAATTGGTAGTAATCGCGGGTCAGCAG\n+ACCGCGGTGAATACGTTCTCAGATCTTGTACacacTGCCCGTCAAGCGAGGAAAGTTGGgggTACCCGAAGC\n+>257959;size=1;\n+TGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGCGAAAGCCCTTTGGGGTGAGTAGAGTGGCGGACGGGTGAGT\n+AATGtgtgGGAATCTGCCTTGCAGTGGgggACAACCCGGGGAAACTCGGGCTAATACCGCATACGTCCTACGGgggAAAG\n+GgggCCGCAAGGCTCTCGCTGCAAGATGAGCCCACATCCGATTAGCTAGTTGGCAGGGTAAGGGCCTACCAAGCCAACGA\n+TCGGTAGCTGGTCTGAGAGGATGACCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGG\n+AATATTGGACAATGGGCGCAAGCCTGATCCAGCAATGCCGCGTgtgtgAAGAAGGCCTGCGGGTTGTAAAGCACTTTCAG\n+TGGGGAAGAACGCCTTAAGGTTAATACCCTTGGgggTTGACGTTACCCACAGAAGAAGCACCGGCTAACTCCGTGCCAGC\n+AGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTACGTAGGCGGCACGGTAAGTCAGA\n+TGTGAAAGCCCCGGGCTCAACCTGGGAACTGCATTTGATACTGTCGCGCTCGAGTCTGAgagagGggggTGGAATTCCAG\n+GTGTAGCGGTGAAATGCGTAGATATCTGGAGGAACACCAGTGGCGAAGGCGGCCcccTGGCTCAAGACTGACGCTGAGGT\n+ACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCTGTAAACGATGTCGACTAGCCGTTGGGTCCAT\n+TTAAGGGCTTAGTGGCGCAGCTAACGCGATAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAAGGAATT\n+GACGGgggCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCAGCCCTTGACATCCTT\n+GGAATCCTGCAGAGATGTGGGAGTGCCTTCGGGAGCCAAGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAG\n+ATGTTGGGTTAAGTCCCGTAACGAGCGCAACCCTTGCCCTTAGTTGCCAGCACGTCATGGTGGGAACTCTAAGGGGACTG\n+CCGGTGATAAACCGGAGGAAGGTGGGGACGACGTCAAGTCATCATGGCCCTTATGGGCTGGGCTACacacGTGCTACAAT\n+GGTCGGTACAGAGGGTCGCAAACCCGCGAGGgggAGCCAATCTCACAAAACCGGTCGTAGTCCGGATCGCAGTCTGCAAC\n+TCGACTGCGTGAAGTCGGAATCGCTAGTAATCGCGAATCAGCATGTCGCGGTGAATACGTTCCCGGGCCTTGTACacacC\n+GCCCGTCACACCATGGGAGTTGGTTGCACCAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCACGGTGTGGTCAA\n+TGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACC\n' |