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Commit message:
Deleted selected files |
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removed:
compStrains.pl |
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| diff -r 8da644edd7f3 -r 57d168d307a8 compStrains.pl --- a/compStrains.pl Wed Mar 29 14:14:00 2017 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 |
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| @@ -1,208 +0,0 @@ -#!/usr/bin/perl -w - -#Margaret Antonio 16.01.13 - -#DESCRIPTION: Takes two aggregate.pl outputs and compares them using mean difference, pval for each -#gene. Can compare, for example, 19F in glucose and TIGR4 in glucose. -#DIFFERENT GENOMES (ie. diff. strains). -#Requires CONVERSION FILE - -#USAGE: perl compStrains.pl -c <conversion.csv> <options> - #[<aggregateFile1.csv aggregateFile2.csv> OR -indir <indir/>] - -use Data::Dumper; -use strict; -use Getopt::Long; -use warnings; -use File::Path; -use File::Basename; -use Statistics::Distributions; - -#ASSIGN INPUTS TO VARIABLES USING FLAGS -our ($input1,$input2,$out,$sortkey,$round,$l1,$l2,$cfile); -GetOptions( -'h' => \$h, -'o:s' =>\$out, -'s:i' => \$sortkey, -'r:i'=> \$round, -'l1:s'=> \$l1, -'l2:s'=> \$l2, -'c:s'=> \$cfile, -'input1:s'=>\$input1, -'input2:s'=>\$input2 -); - -sub print_usage() { - print "\n"; - print "\n##################################################################\n"; - print "compStrains.pl: compare genes from a tn-seq experiment\n"; - print "\tfor two DIFFERENT strains/genomes using aggregate files\n"; - - print "\nDESCRIPTION: Takes two aggregate.pl outputs and compares them by\n"; - print "calculating the difference in mean fitness.\n"; - - print "Example: two strains tested under same condition.\n"; - print "Note: For same strains (genomes), use compGenes.pl\n"; - - print "\nUSAGE:\n"; - print "perl compStrains.pl -c conversion.csv -d inputs/\n"; - - print "\nREQUIRED:\n"; - print " -d\tDirectory containing all input files (files from\n"; - print " \taggregate fitness script)\n"; - print " \tOR\n"; - print " \tIn the command line (without a flag), input the name(s) of\n"; - print " \ttwo files containing aggregate gene fitness values. \n\n"; - print " -c\tConversion file: two columns with homologs for both organisms\n"; - - print "\nOPTIONAL:\n"; - print " -h\tPrints usage and exits program\n"; - print " -o\tOutput file for comparison data. Default: label1label2.csv\n"; - print " -s\tSort output by this index of the file (indices begin at 0).\n"; - print " \tDefault: by mean\n"; - print " -r\tRound final output numbers to this number of decimals\n"; - print " -l\tLabels for input files. Default: filenames\n"; - print " \tTwo strings, comma separated (i.e. -l expt1,expt2).\n"; - print " \tOrder should match file order.\n"; - print " \n~~~~Always check that file paths are correctly specified~~~~\n"; - print "\n##################################################################\n"; -} - -#THE @files ARRAY WILL CONTAIN INPUT FILE NAMES -my @files=($input1,$input2) - -#GET LABELS: -my @labels = ($l1,$l2); - - -#CHECK IF REQ. VARIABLES WERE DEFINED USING FLAGS. IF NOT THEN USE DEFAULT VALUES - -if (!$out) {$out="comp.".$labels[0].$labels[1].".csv"} -if (!$round){$round='%.4f'} - -#OPEN INPUTTED AGGREGATE GENE FILES AND STORE THEIR CONTENTS INTO TWO HASHES -#FILE1 GOES INTO HASH %ONE AND FILE2 GOES INTO HASH %TWO. - -#FILE1 OPENING ---> %one WHERE KEY:VALUE IS GENE_ID:(GENE_ID,INSERTIONS,MEAN,ETC.) -my @header; -my %one; - -open (F1,'<',$files[0]); - -#STORE COLUMN NAMES (FIRST LINE OF FILE1) FOR HEADER AND APPEND LABELS -my $head=<F1>; #the header in the file -my @cols=split(',',$head); -@cols=@cols[0,1,2,3,4,5,6]; #get rid of blank columns -for (my $j=0;$j<scalar @cols;$j++){ - $cols[$j]=$cols[$j].'-'.$labels[0]; #mark each column name with file it comes from -} -push (@header,@cols); - -while (my $line=<F1>){ - chomp $line; - my @info=split(",",$line); - #Only keep the first 7 columns (Ones about blanks aren't needed for comparisons) - @info=@info[0,1,2,3,4,5,6]; - #Sometimes genes that don't have a gene name can't be blank, so fill with NA - if (!$info[5]){ - $info[5]="NA"; - } - #If there are no insertions in the column "total", then make it =0 rather than blank - if (!$info[6]){ - $info[6]=0; - } - $one{$info[0]}=\@info; -} -close F1; - -#FILE2 OPENING ---> %two WHERE KEY:VALUE IS GENE_ID:(GENE_ID,INSERTIONS,MEAN,ETC.) - -my %two; -open (F2,'<',$files[1]); - -#STORE COLUMN NAMES (FIRST LINE OF FILE2) FOR HEADER AND APPEND LABELS -$head=<F2>; #the header in the file -@cols=split(',',$head); -@cols=@cols[0,1,2,3,4,5,6]; #get rid of blank columns -for (my $j=0;$j<scalar @cols;$j++){ - $cols[$j]=$cols[$j].'-'.$labels[1]; #mark each column name with file it comes from -} -push (@header,@cols); - -while (my $line=<F2>){ - chomp $line; - my @info=split(",",$line); - @info=@info[0,1,2,3,4,5,6]; - if (!$info[5]){ - $info[5]="NA"; - } - if (!$info[6]){ - $info[6]=0; - } - $two{$info[0]}=\@info; -} -close F2; - - -#READ CONVERSION FILE INTO ARRAY. -#Conversion file must have strain 1 for file 1 in column 1 (index 0) and - #strain 2 for file 2 in column 2 (index 1) - #conversion file must be tab delimited with no NA fields -#If homologs (exist then take info from hashes (%one and %two) by referring to gene_id in KEY - -my @all; #store all homologs in this hash -open (CONV,'<',$cfile); -while (my $line=<CONV>){ - chomp $line; - my @genes=split("\t",$line); #Array @genes will contain two genes (SP_0000,SPT_0000) - if (scalar @genes==2 and $genes[0] ne "" and $genes[1] ne ""){ - my @info; - my @oneArray=@{$one{$genes[0]}}; - my @twoArray=@{$two{$genes[1]}}; - push (@info,@oneArray,@twoArray); - my $diff=sprintf("$round",($info[1]-$info[8])); - my $total1=$info[6]; - my $total2=$info[13]; - my $sd1=$info[3]; - my $se1=$info[4]; - my $sd2=$info[10]; - my $se2=$info[11]; - my $df=$total1+$total2-2; - my $tdist; - my $pval; - #TDIST, PVAL calculations with fail if standard dev, error, or counts are not real numbers - #or if 0 ends up in denominator - if ($se1 eq "X" or $se2 eq "X" or $sd1 eq "X" or $sd2 eq "X" or $total1==0 or $total2==0 or $sd1==0 or $sd2==0){ - ($tdist,$pval)=("NA","NA"); - } - else{ - $tdist=sqrt((($diff)/(sqrt((($sd1**2)/$total1)+(($sd2**2)/$total2))))**2); - $pval=Statistics::Distributions::tprob($df,$tdist); - } - push (@info,$diff,$df,$tdist,$pval); - push (@all,\@info); - } -} -close CONV; - -#SORT THE HOMOLOGS BY THE SORTKEY OR BY DEFAULT DIFFERENCE IN MEAN FITNESSES -if (!$sortkey){ - $sortkey=14; #for mean difference -} -my @sorted = sort { $b->[$sortkey] <=> $a->[$sortkey] } @all; - -#FINISH THE HEADER BY ADDING COLUMN NAMES FOR MEAN-DIFF, DOF, TDIST, AND PVALUE -my $field="MeanDiff(".$labels[0].'.'.$labels[1].")"; -push (@header,$field,"DOF","TDIST","PVALUE"); - -#PRINT MATCHED HOMOLOG INFORMATION INTO A SINGLE OUTPUT FILE -open OUT, '>',"$out"; -print OUT (join(',',@header),"\n"); -foreach (@sorted){ - my @woo=@{$_}; - print OUT join(',',@woo),"\n"; - } - -close OUT; - - |