Repository 'rseqc'
hg clone https://toolshed.g2.bx.psu.edu/repos/nilesh/rseqc

Changeset 54:5873cd7afb67 (2018-06-13)
Previous changeset 53:080fd5739bb4 (2018-03-20) Next changeset 55:b0b62dcd0a35 (2018-09-18)
Commit message:
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rseqc commit 62a9135bf04aec398d3172d17ccd60f5242d8e82
modified:
FPKM_count.xml
RNA_fragment_size.xml
RPKM_saturation.xml
geneBody_coverage.xml
geneBody_coverage2.xml
infer_experiment.xml
inner_distance.xml
junction_annotation.xml
junction_saturation.xml
read_distribution.xml
rseqc_macros.xml
tin.xml
removed:
test-data/output.RNA_fragment_size.txt
test-data/output.tin.summary.txt
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diff -r 080fd5739bb4 -r 5873cd7afb67 FPKM_count.xml
--- a/FPKM_count.xml Tue Mar 20 05:37:48 2018 -0400
+++ b/FPKM_count.xml Wed Jun 13 18:02:25 2018 -0400
b
@@ -1,4 +1,4 @@
-<tool id="rseqc_FPKM_count" name="FPKM Count" version="@WRAPPER_VERSION@">
+<tool id="rseqc_FPKM_count" name="FPKM Count" version="@WRAPPER_VERSION@.1">
     <description>calculates raw read count, FPM, and FPKM for each gene</description>
 
     <macros>
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diff -r 080fd5739bb4 -r 5873cd7afb67 RNA_fragment_size.xml
--- a/RNA_fragment_size.xml Tue Mar 20 05:37:48 2018 -0400
+++ b/RNA_fragment_size.xml Wed Jun 13 18:02:25 2018 -0400
b
@@ -1,4 +1,4 @@
-<tool id="rseqc_RNA_fragment_size" name="RNA fragment size" version="@WRAPPER_VERSION@">
+<tool id="rseqc_RNA_fragment_size" name="RNA fragment size" version="@WRAPPER_VERSION@.1">
     <description>
      calculates the fragment size for each gene/transcript
     </description>
@@ -35,7 +35,18 @@
         <test>
             <param name="input" value="pairend_strandspecific_51mer_hg19_chr1_1-100000.bam" />
             <param name="refgene" value="hg19_RefSeq_chr1_1-100000.bed" />
-            <output name="output" file="output.RNA_fragment_size.txt" />
+            <output name="output">
+                <assert_contents>
+                    <has_line_matching expression="^chrom\ttx_start\ttx_end\tsymbol\tfrag_count\tfrag_mean\tfrag_median\tfrag_std$" />
+                    <has_line_matching expression="^chr1\t11873\t14409\tNR_046018\t1\t0\t0\t0$" />
+                    <has_line_matching expression="^chr1\t14361\t29370\tNR_024540\t14\t66.5\t51.0\t41.119599080\d+$" />
+                    <has_line_matching expression="^chr1\t17368\t17436\tNR_106918\t0\t0\t0\t0$" />
+                    <has_line_matching expression="^chr1\t17368\t17436\tNR_107062\t0\t0\t0\t0$" />
+                    <has_line_matching expression="^chr1\t34610\t36081\tNR_026818\t0\t0\t0\t0$" />
+                    <has_line_matching expression="^chr1\t34610\t36081\tNR_026820\t0\t0\t0\t0$" />
+                    <has_line_matching expression="^chr1\t69090\t70008\tNM_001005484\t0\t0\t0\t0$" />
+                </assert_contents>
+            </output>
         </test>
     </tests>
 
b
diff -r 080fd5739bb4 -r 5873cd7afb67 RPKM_saturation.xml
--- a/RPKM_saturation.xml Tue Mar 20 05:37:48 2018 -0400
+++ b/RPKM_saturation.xml Wed Jun 13 18:02:25 2018 -0400
b
@@ -1,4 +1,4 @@
-<tool id="rseqc_RPKM_saturation" name="RPKM Saturation" version="@WRAPPER_VERSION@">
+<tool id="rseqc_RPKM_saturation" name="RPKM Saturation" version="@WRAPPER_VERSION@.1">
     <description>calculates raw count and RPKM values for transcript at exon, intron, and mRNA level</description>
 
     <macros>
b
diff -r 080fd5739bb4 -r 5873cd7afb67 geneBody_coverage.xml
--- a/geneBody_coverage.xml Tue Mar 20 05:37:48 2018 -0400
+++ b/geneBody_coverage.xml Wed Jun 13 18:02:25 2018 -0400
b
@@ -1,4 +1,4 @@
-<tool id="rseqc_geneBody_coverage" name="Gene Body Converage (BAM)" version="@WRAPPER_VERSION@.1">
+<tool id="rseqc_geneBody_coverage" name="Gene Body Converage (BAM)" version="@WRAPPER_VERSION@.2">
   <description>
     Read coverage over gene body.
   </description>
b
diff -r 080fd5739bb4 -r 5873cd7afb67 geneBody_coverage2.xml
--- a/geneBody_coverage2.xml Tue Mar 20 05:37:48 2018 -0400
+++ b/geneBody_coverage2.xml Wed Jun 13 18:02:25 2018 -0400
b
@@ -1,4 +1,4 @@
-<tool id="rseqc_geneBody_coverage2" name="Gene Body Converage (Bigwig)" version="@WRAPPER_VERSION@">
+<tool id="rseqc_geneBody_coverage2" name="Gene Body Converage (Bigwig)" version="@WRAPPER_VERSION@.1">
     <description>
         Read coverage over gene body
     </description>
b
diff -r 080fd5739bb4 -r 5873cd7afb67 infer_experiment.xml
--- a/infer_experiment.xml Tue Mar 20 05:37:48 2018 -0400
+++ b/infer_experiment.xml Wed Jun 13 18:02:25 2018 -0400
b
@@ -1,4 +1,4 @@
-<tool id="rseqc_infer_experiment" name="Infer Experiment" version="@WRAPPER_VERSION@">
+<tool id="rseqc_infer_experiment" name="Infer Experiment" version="@WRAPPER_VERSION@.1">
     <description>speculates how RNA-seq were configured</description>
 
     <macros>
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diff -r 080fd5739bb4 -r 5873cd7afb67 inner_distance.xml
--- a/inner_distance.xml Tue Mar 20 05:37:48 2018 -0400
+++ b/inner_distance.xml Wed Jun 13 18:02:25 2018 -0400
b
@@ -1,4 +1,4 @@
-<tool id="rseqc_inner_distance" name="Inner Distance" version="@WRAPPER_VERSION@">
+<tool id="rseqc_inner_distance" name="Inner Distance" version="@WRAPPER_VERSION@.1">
     <description>calculate the inner distance (or insert size) between two paired RNA reads</description>
 
     <macros>
b
diff -r 080fd5739bb4 -r 5873cd7afb67 junction_annotation.xml
--- a/junction_annotation.xml Tue Mar 20 05:37:48 2018 -0400
+++ b/junction_annotation.xml Wed Jun 13 18:02:25 2018 -0400
b
@@ -1,4 +1,4 @@
-<tool id="rseqc_junction_annotation" name="Junction Annotation" version="@WRAPPER_VERSION@">
+<tool id="rseqc_junction_annotation" name="Junction Annotation" version="@WRAPPER_VERSION@.1">
     <description>compares detected splice junctions to reference gene model</description>
 
     <macros>
b
diff -r 080fd5739bb4 -r 5873cd7afb67 junction_saturation.xml
--- a/junction_saturation.xml Tue Mar 20 05:37:48 2018 -0400
+++ b/junction_saturation.xml Wed Jun 13 18:02:25 2018 -0400
b
@@ -1,4 +1,4 @@
-<tool id="rseqc_junction_saturation" name="Junction Saturation" version="@WRAPPER_VERSION@">
+<tool id="rseqc_junction_saturation" name="Junction Saturation" version="@WRAPPER_VERSION@.1">
     <description>detects splice junctions from each subset and compares them to reference gene model</description>
 
     <macros>
b
diff -r 080fd5739bb4 -r 5873cd7afb67 read_distribution.xml
--- a/read_distribution.xml Tue Mar 20 05:37:48 2018 -0400
+++ b/read_distribution.xml Wed Jun 13 18:02:25 2018 -0400
b
@@ -1,4 +1,4 @@
-<tool id="rseqc_read_distribution" name="Read Distribution" version="@WRAPPER_VERSION@">
+<tool id="rseqc_read_distribution" name="Read Distribution" version="@WRAPPER_VERSION@.1">
     <description>calculates how mapped reads were distributed over genome feature</description>
 
     <macros>
b
diff -r 080fd5739bb4 -r 5873cd7afb67 rseqc_macros.xml
--- a/rseqc_macros.xml Tue Mar 20 05:37:48 2018 -0400
+++ b/rseqc_macros.xml Wed Jun 13 18:02:25 2018 -0400
b
@@ -26,7 +26,7 @@
     </xml>
 
     <xml name="refgene_param">
-        <param name="refgene" type="data" format="bed" label="Reference gene model" help="(--refgene)"/>
+        <param name="refgene" type="data" format="bed12" label="Reference gene model" help="(--refgene)"/>
     </xml>
 
     <xml name="mapq_param">
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diff -r 080fd5739bb4 -r 5873cd7afb67 test-data/output.RNA_fragment_size.txt
--- a/test-data/output.RNA_fragment_size.txt Tue Mar 20 05:37:48 2018 -0400
+++ /dev/null Thu Jan 01 00:00:00 1970 +0000
b
@@ -1,8 +0,0 @@
-chrom tx_start tx_end symbol frag_count frag_mean frag_median frag_std
-chr1 11873 14409 NR_046018 1 0 0 0
-chr1 14361 29370 NR_024540 14 66.5 51.0 41.119599080869314
-chr1 17368 17436 NR_106918 0 0 0 0
-chr1 17368 17436 NR_107062 0 0 0 0
-chr1 34610 36081 NR_026818 0 0 0 0
-chr1 34610 36081 NR_026820 0 0 0 0
-chr1 69090 70008 NM_001005484 0 0 0 0
b
diff -r 080fd5739bb4 -r 5873cd7afb67 test-data/output.tin.summary.txt
--- a/test-data/output.tin.summary.txt Tue Mar 20 05:37:48 2018 -0400
+++ /dev/null Thu Jan 01 00:00:00 1970 +0000
b
@@ -1,2 +0,0 @@
-Bam_file TIN(mean) TIN(median) TIN(stdev)
-input.bam 8.870967741935486 8.870967741935486 0.0
b
diff -r 080fd5739bb4 -r 5873cd7afb67 tin.xml
--- a/tin.xml Tue Mar 20 05:37:48 2018 -0400
+++ b/tin.xml Wed Jun 13 18:02:25 2018 -0400
b
@@ -1,4 +1,4 @@
-<tool id="rseqc_tin" name="Transcript Integrity Number" version="@WRAPPER_VERSION@">
+<tool id="rseqc_tin" name="Transcript Integrity Number" version="@WRAPPER_VERSION@.1">
     <description>
         evaluates RNA integrity at a transcript level
     </description>
@@ -50,7 +50,12 @@
         <test>
             <param name="input" value="pairend_strandspecific_51mer_hg19_chr1_1-100000.bam"/>
             <param name="refgene" value="hg19_RefSeq_chr1_1-100000.bed"/>
-            <output name="outputsummary" file="output.tin.summary.txt"/>
+            <output name="outputsummary">
+                <assert_contents>
+                    <has_line_matching expression="^Bam_file\tTIN\(mean\)\tTIN\(median\)\tTIN\(stdev\)$" />
+                    <has_line_matching expression="^input\.bam\t8\.8709677419\d+\t8\.8709677419\d+\t0\.0$" />
+                </assert_contents>
+            </output>
             <output name="outputxls" file="output.tin.xls"/>
         </test>
     </tests>