Repository 'rgrnastar'
hg clone https://toolshed.g2.bx.psu.edu/repos/iuc/rgrnastar

Changeset 15:5ec75f5dae3c (2020-08-05)
Previous changeset 14:b9e04854e2bb (2019-11-28) Next changeset 16:e132e7d02a3e (2020-12-04)
Commit message:
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar commit a8e319862d723654c372a6d71e5de76e052586a9"
modified:
macros.xml
rg_rnaStar.xml
test-data/test-cache/tophat-test/genomeParameters.txt
tool_data_table_conf.xml.sample
tool_data_table_conf.xml.test
added:
test-data/filtered.barcodes.txt
test-data/filtered3.Homo_sapiens.GRCh38.100.chr21.gtf
test-data/filtered3.Homo_sapiens.GRCh38.dna.chromosome.21.fa.gz
test-data/filtered3.bam
test-data/pbmc_1k_v2_L001.R1.10k.fastq.gz
test-data/pbmc_1k_v2_L001.R2.10k.fastq.gz
test-data/rnastar_index2x_versioned.loc
test-data/test-cache/tophat-test/Log.out
tool-data/rnastar_index2x_versioned.loc.sample
removed:
test-data/41737_R1_sub240k.fastq.gz
test-data/41737_R2_sub240k.fastq.gz
test-data/737K-august-2016.small.txt.gz
test-data/SNORD83B.22.fa
test-data/SNORD83B.22.gtf
test-data/rnastar_index2_versioned.loc
tool-data/rnastar_index2_versioned.loc.sample
b
diff -r b9e04854e2bb -r 5ec75f5dae3c macros.xml
--- a/macros.xml Thu Nov 28 15:56:07 2019 -0500
+++ b/macros.xml Wed Aug 05 09:40:33 2020 -0400
b
@@ -5,7 +5,7 @@
     the index versions in sync, but you should manually adjust the +galaxy
     version number. -->
     <!-- STAR version to be used -->
-    <token name="@VERSION@">2.7.2b</token>
+    <token name="@VERSION@">2.7.5b</token>
     <!-- STAR index version compatible with this version of STAR
     This is the STAR version that introduced the index structure expected
     by the current version.
@@ -13,7 +13,8 @@
     STAR -h | grep versionGenome
     or by looking for the versionGenome parameter in source/parametersDefault
     of STAR's source code -->
-    <token name="@IDX_VERSION@">2.7.1a</token>
+    <token name="@IDX_VERSION@">2.7.4a</token>
+    <token name="@IDX_DATA_TABLE@">rnastar_index2x_versioned</token>
 
     <xml name="requirements">
         <requirements>
@@ -27,7 +28,7 @@
         <param argument="--genomeDir" name="genomeDir" type="select"
         label="Select reference genome"
         help="If your genome of interest is not listed, contact the Galaxy team">
-            <options from_data_table="rnastar_index2_versioned">
+            <options from_data_table="@IDX_DATA_TABLE@">
                 <filter type="static_value" column="4" value="@WITH_GENE_MODEL@" />
                 <filter type="static_value" column="5" value="@IDX_VERSION@" />
                 <filter type="sort_by" column="2" />
@@ -53,7 +54,7 @@
             <conditional name="refGenomeSource.geneSource">
                 <when value="indexed">
                     <action type="metadata" name="dbkey">
-                        <option type="from_data_table" name="rnastar_index2_versioned" column="1" offset="0">
+                        <option type="from_data_table" name="@IDX_DATA_TABLE@" column="1" offset="0">
                             <filter type="param_value" column="0" value="#" compare="startswith" keep="False"/>
                             <filter type="param_value" ref="refGenomeSource.GTFconditional.genomeDir" column="0"/>
                         </option>
@@ -76,10 +77,21 @@
             --genomeDir 'tempstargenomedir'
             --genomeFastaFiles '${refGenomeSource.genomeFastaFiles}'
             ## Handle difference between indices with/without annotations
-            #if str($refGenomeSource.GTFconditional.GTFselect) == 'with-gtf':
-                --sjdbOverhang '${refGenomeSource.GTFconditional.sjdbOverhang}'
-                --sjdbGTFfile '${refGenomeSource.GTFconditional.sjdbGTFfile}'
-                #if str($refGenomeSource.GTFconditional.sjdbGTFfile.ext) == 'gff3':
+            #if 'GTFconditional' in $refGenomeSource:
+                ## GTFconditional exists only in STAR, but not STARsolo
+                #if str($refGenomeSource.GTFconditional.GTFselect) == 'with-gtf':
+                    --sjdbOverhang '${refGenomeSource.GTFconditional.sjdbOverhang}'
+                    --sjdbGTFfile '${refGenomeSource.GTFconditional.sjdbGTFfile}'
+                    #if str($refGenomeSource.GTFconditional.sjdbGTFfile.ext) == 'gff3':
+                        --sjdbGTFtagExonParentTranscript Parent
+                    #end if
+                #end if
+            #else:
+                ## ref genome selection is less complex for STARsolo cause
+                ## with-gtf is mandatory there
+                --sjdbOverhang '${refGenomeSource.sjdbOverhang}'
+                --sjdbGTFfile '${refGenomeSource.sjdbGTFfile}'
+                #if str($refGenomeSource.sjdbGTFfile.ext) == 'gff3':
                     --sjdbGTFtagExonParentTranscript Parent
                 #end if
             #end if
@@ -110,6 +122,13 @@
         #end if
         #end if
         ]]></token>
+    <xml name="ref_selection">
+        <param argument="--genomeFastaFiles" type="data" format="fasta" label="Select a reference genome" />
+        <!-- Currently, this parameter is not exposed in the wrapper,
+             but used only in the tests to avoid excessive index sizes for
+             the tiny test genomes. -->
+        <param name="genomeSAindexNbases" type="hidden" value="" />
+    </xml>
     <xml name="stdio" >
         <stdio>
             <regex match="FATAL error" source="both" level="fatal"/>
@@ -119,48 +138,4 @@
             <yield />
         </stdio>
     </xml>
-    <xml name="refgenomehandling" >
-        <conditional name="refGenomeSource">
-            <param name="geneSource" type="select" label="Custom or built-in reference genome" help="Built-ins were indexed using default options">
-                <option value="indexed" selected="true">Use a built-in index</option>
-                <option value="history">Use reference genome from history and create temporary index</option>
-            </param>
-            <when value="indexed">
-                <conditional name="GTFconditional">
-                    <param name="GTFselect" type="select"
-                           label="Reference genome with or without an annotation"
-                           help="Select the '... with builtin gene-model' option to select from the list of available indexes that were built with splice junction information. Select the '... without builtin gene-model' option to select from the list of available indexes without annotated splice junctions.">
-                        <option value="without-gtf" selected='true'>use genome reference without builtin gene-model</option>
-                        <option value="with-gtf">use genome reference with builtin gene-model</option>
-                    </param>
-                    <when value="with-gtf">
-                        <expand macro="index_selection" with_gene_model="1" />
-                    </when>
-                    <when value="without-gtf">
-                        <expand macro="index_selection" with_gene_model="0" />
-                        <expand macro="@SJDBOPTIONS@" />
-                    </when>
-                </conditional>
-            </when>
-            <when value="history">
-                <param argument="--genomeFastaFiles" type="data" format="fasta" label="Select a reference genome" />
-                <!-- Currently, this parameter is not exposed in the wrapper,
-                     but used only in the tests to avoid excessive index sizes for
-                     the tiny test genomes. -->
-                <param name="genomeSAindexNbases" type="hidden" value="" />
-                <conditional name="GTFconditional">
-                    <param name="GTFselect" type="select"
-                           label="Build index with our without known splice junctions annotation"
-                           help="To build an index with known splice junctions annotated, you will have to provide a GTF or GFF3 dataset that describes the gene models (the location of genes, transcripts and exons) known for the reference genome.">
-                        <option value="without-gtf">build index without gene-model</option>
-                        <option value="with-gtf">build index with gene-model</option>
-                    </param>
-                    <when value="with-gtf">
-                        <expand macro="@SJDBOPTIONS@" optional="false"/>
-                    </when>
-                    <when value="without-gtf" />
-                </conditional>
-            </when>
-        </conditional>
-    </xml>
 </macros>
b
diff -r b9e04854e2bb -r 5ec75f5dae3c rg_rnaStar.xml
--- a/rg_rnaStar.xml Thu Nov 28 15:56:07 2019 -0500
+++ b/rg_rnaStar.xml Wed Aug 05 09:40:33 2020 -0400
b
@@ -246,7 +246,44 @@
         </conditional>
 
         <!-- Genome source. -->
-        <expand macro="refgenomehandling" />
+        <conditional name="refGenomeSource">
+            <param name="geneSource" type="select" label="Custom or built-in reference genome" help="Built-ins were indexed using default options">
+                <option value="indexed" selected="true">Use a built-in index</option>
+                <option value="history">Use reference genome from history and create temporary index</option>
+            </param>
+            <when value="indexed">
+                <conditional name="GTFconditional">
+                    <param name="GTFselect" type="select"
+                           label="Reference genome with or without an annotation"
+                           help="Select the '... with builtin gene-model' option to select from the list of available indexes that were built with splice junction information. Select the '... without builtin gene-model' option to select from the list of available indexes without annotated splice junctions, and, optionally, provide your own splice-junction annonations.">
+                        <option value="without-gtf" selected='true'>use genome reference without builtin gene-model</option>
+                        <option value="with-gtf">use genome reference with builtin gene-model</option>
+                    </param>
+                    <when value="with-gtf">
+                        <expand macro="index_selection" with_gene_model="1" />
+                    </when>
+                    <when value="without-gtf">
+                        <expand macro="index_selection" with_gene_model="0" />
+                        <expand macro="@SJDBOPTIONS@" />
+                    </when>
+                </conditional>
+            </when>
+            <when value="history">
+                <expand macro="ref_selection" />
+                <conditional name="GTFconditional">
+                    <param name="GTFselect" type="select"
+                           label="Build index with or without known splice junctions annotation"
+                           help="To build an index with known splice junctions annotated, you will have to provide a GTF or GFF3 dataset that describes the gene models (the location of genes, transcripts and exons) known for the reference genome.">
+                        <option value="without-gtf">build index without gene-model</option>
+                        <option value="with-gtf">build index with gene-model</option>
+                    </param>
+                    <when value="with-gtf">
+                        <expand macro="@SJDBOPTIONS@" optional="false"/>
+                    </when>
+                    <when value="without-gtf" />
+                </conditional>
+            </when>
+        </conditional>
 
         <conditional name="twopass">
             <param argument="--twopassMode" type="select"
b
diff -r b9e04854e2bb -r 5ec75f5dae3c test-data/41737_R1_sub240k.fastq.gz
b
Binary file test-data/41737_R1_sub240k.fastq.gz has changed
b
diff -r b9e04854e2bb -r 5ec75f5dae3c test-data/41737_R2_sub240k.fastq.gz
b
Binary file test-data/41737_R2_sub240k.fastq.gz has changed
b
diff -r b9e04854e2bb -r 5ec75f5dae3c test-data/737K-august-2016.small.txt.gz
b
Binary file test-data/737K-august-2016.small.txt.gz has changed
b
diff -r b9e04854e2bb -r 5ec75f5dae3c test-data/SNORD83B.22.fa
--- a/test-data/SNORD83B.22.fa Thu Nov 28 15:56:07 2019 -0500
+++ /dev/null Thu Jan 01 00:00:00 1970 +0000
b
@@ -1,17 +0,0 @@
->22 dna:chromosome chromosome:GRCh38:22:39313740:50818468:1 REF
-CACACTCAAAGCAGCAAACAGCCCAGCAAGGCCAGACTGGGAATTTCCTCATCTCAGGAC
-TTCAAAGCCAGTGTGAAAGGACTGCCAACACCCTCTCCTTCCTTTCCTCTCCCACCACAG
-GGCCACCAGCGTCTGTGGCCTTGGATCCTCCCTCTACAAGAGCCCCCCCATGACAAGTCA
-GGACCTGCCTCACCTTGCGGAGGGTGAGCACCCGCTTCTTGGTTCCCACCACACAGCCTT
-TCAGCATGACAAAGTCATTGGTCACTTCACCATAGTGGACAAAGCCACCCTGGAAAACGA
-GCATCGGATCAGCACAGGCCCAGGAGGGGATTGTCGTGCAGATGACCCCTCCAGGTTCAG
-GCCCTCCCTGACCACAGGGCTGTTCTCAGAAGGAAGGCAACAAGGAACGGTTCCGCAGTC
-TGTCTCGGGCGCTGTGCCCAGCGCACATTCCAGGCCTCATCACTGAACAGCTGAGCCTGA
-GACCCCACTTCTCACCAGCCAACCCCGACGAGTGGACTCAGATGACAACATGCCACTTAC
-AAGGGACACAGCTAGGTGTTGTGTTGGCTTCAGTTAACGATCCTGCTAGCAGCCCCTAGG
-AAGCAGCCTATCCCCAAAAGCACGAGGCCTGGGATGGCCTCACAGAGCAGAACACCCATT
-ACTTACCAGAGGGTTGATGCTCTTGTCAGATAGGTCATAGTCAGTGGAGGCATTGTTCTT
-GATCAGCTTGCCGTCCTTGATAAGGTAGCCCTGGCCAATCTTATAAATCTGAATGAACAA
-GAAGGGTGTAAGGCTGGGGCATTAGGGACAAATAACCCAGACATGCCAGTGTGCTGACCT
-GCAAAGCACGCTAGAAGGCAGCTGAGGCCTCAGTCCCAGTCACAGCGTATCCCAAGGTCA
-GAGCAAAAAGCTGGCTGGCCCTCCAGGTTCCTTTCTGTAAGGCGGCTGGGCTAAAACTAA
\ No newline at end of file
b
diff -r b9e04854e2bb -r 5ec75f5dae3c test-data/SNORD83B.22.gtf
--- a/test-data/SNORD83B.22.gtf Thu Nov 28 15:56:07 2019 -0500
+++ /dev/null Thu Jan 01 00:00:00 1970 +0000
b
@@ -1,8 +0,0 @@
-#!genome-build GRCh38.p12
-#!genome-version GRCh38
-#!genome-date 2013-12
-#!genome-build-accession NCBI:GCA_000001405.27
-#!genebuild-last-updated 2018-11
-22 ensembl gene 39313819 39313911 . - . gene_id "ENSG00000209480"; gene_version "1"; gene_name "SNORD83B"; gene_source "ensembl"; gene_biotype "snoRNA";
-22 ensembl transcript 39313819 39313911 . - . gene_id "ENSG00000209480"; gene_version "1"; transcript_id "ENST00000386745"; transcript_version "1"; gene_name "SNORD83B"; gene_source "ensembl"; gene_biotype "snoRNA"; transcript_name "SNORD83B-201"; transcript_source "ensembl"; transcript_biotype "snoRNA"; tag "basic"; transcript_support_level "NA";
-22 ensembl exon 39313819 39313911 . - . gene_id "ENSG00000209480"; gene_version "1"; transcript_id "ENST00000386745"; transcript_version "1"; exon_number "1"; gene_name "SNORD83B"; gene_source "ensembl"; gene_biotype "snoRNA"; transcript_name "SNORD83B-201"; transcript_source "ensembl"; transcript_biotype "snoRNA"; exon_id "ENSE00001501751"; exon_version "1"; tag "basic"; transcript_support_level "NA";
b
diff -r b9e04854e2bb -r 5ec75f5dae3c test-data/filtered.barcodes.txt
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/filtered.barcodes.txt Wed Aug 05 09:40:33 2020 -0400
b
@@ -0,0 +1,394 @@
+AAACCTGAGCGCTCCA
+AAACGGGGTTTGTGTG
+AAAGATGAGTACTTGC
+AAAGCAATCCACGAAT
+AAAGTAGGTAGCAAAT
+AACCATGGTAGTACCT
+AACCGCGCACATCTTT
+AACCGCGCATGCCTAA
+AACGTTGTCACAGTAC
+AACTTTCTCTGTTTGT
+AAGACCTTCAAACGGG
+AAGGAGCAGTATCGAA
+AAGGAGCAGTGGTAGC
+AAGGAGCCAGCTTAAC
+AAGGAGCTCTAAGCCA
+ACACCAACAGCTGTAT
+ACACCGGTCTAACGGT
+ACATACGCACACCGCA
+ACATACGTCTGCCCTA
+ACATACGTCTGGCGAC
+ACATCAGCAAACGCGA
+ACATGGTCAGACTCGC
+ACCAGTAAGCCTCGTG
+ACCGTAACACAACGCC
+ACCGTAAGTTCAGTAC
+ACCGTAATCTTGGGTA
+ACGAGCCCAAGAGTCG
+ACGAGCCGTGCTGTAT
+ACGAGGATCCTATGTT
+ACGAGGATCTTTAGTC
+ACGATACCAAAGTCAA
+ACGCAGCTCACCGTAA
+ACGCCGAAGCGCTTAT
+ACGGAGACATCTATGG
+ACGGCCACACCGAAAG
+ACGGGCTGTCGGCACT
+ACGGGTCCACACCGCA
+ACTGAGTAGACGACGT
+ACTGAGTTCGGAGCAA
+ACTGAGTTCGTCTGAA
+ACTGATGCAAGTTCTG
+ACTGCTCGTGCAGGTA
+ACTGTCCAGAGCTGCA
+ACTGTCCTCCCACTTG
+ACTTACTAGTTAGCGG
+ACTTACTGTCCCTTGT
+ACTTACTGTTACCAGT
+ACTTTCAGTCTCCACT
+ACTTTCAGTTAAAGTG
+AGAATAGCAGCTGTAT
+AGACGTTCAAGGCTCC
+AGACGTTCAGACTCGC
+AGACGTTGTCAAAGCG
+AGACGTTTCAGTTTGG
+AGAGCTTGTGACGCCT
+AGAGTGGCAGATGAGC
+AGAGTGGTCTTTAGGG
+AGCATACCAGGGTATG
+AGCCTAAGTTTAGGAA
+AGCCTAATCCCGACTT
+AGCGGTCAGTACATGA
+AGCGGTCGTTCCACGG
+AGCTCCTGTGTTTGGT
+AGCTCTCAGCAGCCTC
+AGCTCTCAGTGTTAGA
+AGGCCACGTCAGCTAT
+AGGCCACGTGCAGGTA
+AGGCCACTCGTGGTCG
+AGGGAGTAGGGTCTCC
+AGGGAGTTCGATGAGG
+AGGGATGGTTCAGGCC
+AGGTCATAGCTCCTCT
+AGGTCATCATCCGTGG
+AGTAGTCAGGATGTAT
+AGTAGTCCAATGGATA
+AGTCTTTAGCAGGCTA
+AGTGAGGCAATCGGTT
+AGTGAGGTCGAACGGA
+AGTGTCAAGGCGATAC
+AGTGTCAGTTCCATGA
+ATAACGCGTCGGCACT
+ATAAGAGCACCAGGTC
+ATAAGAGCACTTCTGC
+ATAAGAGTCGGGAGTA
+ATAGACCGTCCTAGCG
+ATCACGACATATGCTG
+ATCACGAGTCGACTGC
+ATCATGGTCCGTACAA
+ATCCACCCATTACGAC
+ATCGAGTCAGGTCTCG
+ATCGAGTGTTCCGTCT
+ATCTGCCCACCAGGCT
+ATCTGCCTCGCCATAA
+ATGAGGGGTCTTCAAG
+ATGTGTGAGGCTCAGA
+ATTACTCAGACCCACC
+ATTCTACAGTTTCCTT
+ATTCTACTCATTGCGA
+ATTTCTGTCTAACTCT
+CAACCAAGTCCGTCAG
+CAACTAGTCATGGTCA
+CAAGAAAGTTCCGTCT
+CAAGATCTCGTAGGTT
+CAAGGCCCAATCTACG
+CAAGGCCGTTATTCTC
+CAAGTTGTCGTACGGC
+CACAAACAGCCCAATT
+CACACCTTCCCAACGG
+CACACTCCATATGAGA
+CACAGGCCAGTCCTTC
+CACAGGCGTCATGCCG
+CACAGTAAGCTGTCTA
+CACAGTACAGTCGTGC
+CACATAGTCGTACCGG
+CACCACTAGTGTACCT
+CACCACTCATACGCCG
+CACCTTGAGTGTACGG
+CAGAATCGTGAAGGCT
+CAGAATCGTTACGACT
+CAGAGAGTCTTGTACT
+CAGCAGCGTGCAACGA
+CAGCATAAGTGGCACA
+CAGCCGAGTTCTGAAC
+CAGCTAACACCTCGTT
+CAGCTAAGTCAGTGGA
+CATATTCCACCTCGTT
+CATCAAGAGACAGAGA
+CATCCACAGGCGACAT
+CATCCACTCTGCCAGG
+CATGCCTGTTCTCATT
+CCAATCCAGACCTAGG
+CCACCTACAGAAGCAC
+CCACCTACAGCCTGTG
+CCCAATCTCCTAAGTG
+CCCAGTTAGTCCAGGA
+CCCAGTTCAAGGTTCT
+CCCATACAGCACCGCT
+CCCATACGTACCGCTG
+CCCATACGTGTTTGTG
+CCCTCCTGTGTGCCTG
+CCCTCCTTCGGTCCGA
+CCGGTAGGTGCAGTAG
+CCGGTAGTCTTCCTTC
+CCGTACTCATGGTCTA
+CCGTACTTCACTTCAT
+CCTAAAGGTGAGGGAG
+CCTACCAAGTGGGATC
+CCTACCAGTAAGTGGC
+CCTAGCTAGTCAAGCG
+CCTAGCTGTTTGCATG
+CGACCTTAGCCGTCGT
+CGACCTTAGCGATCCC
+CGACCTTTCTGTCAAG
+CGACTTCGTTCGAATC
+CGACTTCGTTGTGGCC
+CGACTTCTCATATCGG
+CGAGAAGTCTTGCATT
+CGAGCACAGAAGGTTT
+CGAGCACCAACGATGG
+CGAGCACTCAGAAATG
+CGAGCCACACCAGCAC
+CGATCGGCATAACCTG
+CGATGGCGTGCACGAA
+CGATGGCGTGGTTTCA
+CGCGGTACAGTATGCT
+CGCGGTACATGGATGG
+CGCTATCTCATCTGTT
+CGCTGGAAGGAGCGTT
+CGCTTCAAGTACGCGA
+CGCTTCAAGTTCGCGC
+CGCTTCACAATCAGAA
+CGGACACTCTATCCCG
+CGGACGTTCAGATAAG
+CGGACGTTCTCATTCA
+CGGACTGTCTAACTCT
+CGGAGCTCACGAAGCA
+CGGAGTCTCAACACCA
+CGGGTCAGTCGACTAT
+CGGGTCATCATCGCTC
+CGGTTAAGTTCAGTAC
+CGGTTAAGTTGTGGCC
+CGTCACTTCGAATGGG
+CGTGTCTAGACTGTAA
+CGTGTCTAGATAGCAT
+CGTTAGACAAGGGTCA
+CGTTCTGGTACATGTC
+CTAACTTGTATGCTTG
+CTAACTTTCTAACCGA
+CTAAGACGTTTGTGTG
+CTACATTAGAAACGCC
+CTACATTAGGTGACCA
+CTACATTTCTCGAGTA
+CTAGAGTAGACACGAC
+CTAGAGTGTCGCGAAA
+CTCACACCATTAACCG
+CTCACACGTGCTTCTC
+CTCAGAATCGGATGGA
+CTCATTATCTAGAGTC
+CTCCTAGTCACGCATA
+CTCGGAGAGCTGAAAT
+CTCGGAGTCTTAGAGC
+CTCGGGACACACGCTG
+CTCGGGATCGTAGGAG
+CTCGTACAGGTAAACT
+CTCTAATGTCCAGTTA
+CTCTGGTAGAGTGACC
+CTGAAGTCAATTGCTG
+CTGAAGTGTATAATGG
+CTGAAGTGTGTCGCTG
+CTGATCCAGTAGCGGT
+CTGATCCTCGTCACGG
+CTGCCTAGTTCGTGAT
+CTGCGGAAGACTAGAT
+CTGCTGTCAAGGTTCT
+CTGCTGTCATTATCTC
+CTGCTGTTCAACTCTT
+CTGGTCTTCATAAAGG
+CTGTGCTAGCAGGTCA
+CTGTGCTCATATACGC
+CTGTTTAAGGGATGGG
+CTTACCGGTGGACGAT
+CTTAGGACAAACCTAC
+CTTCTCTAGATGGCGT
+CTTGGCTAGTCAAGGC
+CTTTGCGAGATCCTGT
+CTTTGCGGTTTAAGCC
+CTTTGCGGTTTGACAC
+GAAATGATCTCCTATA
+GAATAAGGTCAAAGAT
+GAATGAATCATCACCC
+GACCAATCAGCTGGCT
+GACCAATGTCATGCAT
+GACGCGTTCCGATATG
+GACGGCTCATGATCCA
+GACGTGCCATACGCTA
+GACTGCGCAATGGACG
+GACTGCGCATCTACGA
+GACTGCGGTCGGCACT
+GATCGCGTCGTAGGTT
+GATCTAGGTCTAGCGC
+GATGAAATCACAACGT
+GATGCTAGTATATGGA
+GATTCAGCATGCGCAC
+GCAAACTAGAGGGCTT
+GCAAACTGTGTCAATC
+GCAAACTTCTGCGTAA
+GCACTCTAGCACAGGT
+GCAGCCACAGGACCCT
+GCAGCCATCCTCAATT
+GCATGATGTCAACATC
+GCATGTACACAGCGTC
+GCATGTAGTTCAACCA
+GCATGTAGTTGCCTCT
+GCGACCACAGACAAAT
+GCGAGAACAGATGGGT
+GCGCAACCACCTGGTG
+GCGCAACTCGTCACGG
+GCGCAGTAGGAGTACC
+GCGCAGTGTTCGCTAA
+GCGCGATAGGATGGAA
+GCGCGATGTAGCTTGT
+GCGGGTTCATCCGTGG
+GCGGGTTTCGTTTATC
+GCTCTGTGTGCCTGGT
+GCTGCAGTCTGAGTGT
+GCTTCCAAGCAGGCTA
+GCTTCCAGTGCAGTAG
+GCTTGAAAGAAGGACA
+GGAATAACACCAGGTC
+GGACAAGAGTACGCCC
+GGACATTCACTGCCAG
+GGACATTTCACAGTAC
+GGACGTCAGTGGGATC
+GGATGTTTCCACTGGG
+GGCAATTCACCATCCT
+GGCGACTTCAACACCA
+GGCTGGTTCTCGTTTA
+GGGAATGAGGGTTTCT
+GGGACCTCAGTCGATT
+GGGACCTTCGGAGGTA
+GGGATGAGTGCAGACA
+GGTGCGTTCAGCTCGG
+GGTGCGTTCGCTAGCG
+GTACGTAGTATCAGTC
+GTACTCCCACAGACAG
+GTAGGCCCACTGCCAG
+GTATCTTAGACGCTTT
+GTATCTTTCCTCAACC
+GTATTCTAGGTTCCTA
+GTCACAATCTCAACTT
+GTCACGGAGCACACAG
+GTCATTTGTGCTTCTC
+GTGCAGCTCTTCATGT
+GTGCATAAGGAGCGTT
+GTGCGGTAGCTACCGC
+GTGCGGTAGGCCGAAT
+GTGCGGTCATGCCACG
+GTGCTTCAGATGAGAG
+GTGGGTCCATCGGGTC
+GTGGGTCGTTCGAATC
+GTTAAGCAGCCACCTG
+GTTAAGCAGTACATGA
+GTTAAGCCAATTGCTG
+GTTACAGGTCAGGACA
+GTTACAGTCCAGTAGT
+GTTCTCGTCTAACTGG
+TAAACCGCAAGGACAC
+TAAACCGCATACGCCG
+TAAACCGCATGGTAGG
+TAAGCGTAGCTCCTCT
+TACACGATCGTGGGAA
+TACAGTGAGTGTCCCG
+TACAGTGCAGCTCGCA
+TACGGATCAGCTATTG
+TACGGGCCACAACGTT
+TACGGTAAGGATGCGT
+TACTTACAGGCTATCT
+TACTTGTAGACTTTCG
+TACTTGTCACATAACC
+TAGCCGGCAGGGTTAG
+TATGCCCCAATGTAAG
+TATGCCCGTACTTAGC
+TCAACGAAGCTAGTGG
+TCAATCTAGGATGCGT
+TCACAAGCATCCTTGC
+TCACGAATCACTCCTG
+TCAGATGTCGTTGCCT
+TCAGCAATCAGCACAT
+TCAGGATGTATAGGGC
+TCGCGAGGTTCCAACA
+TCGCGAGGTTGTCTTT
+TCGTACCAGGCTCAGA
+TCGTAGAGTCTTGTCC
+TCTATTGCATGCCTAA
+TCTCTAACAGATCCAT
+TCTTTCCTCCACTCCA
+TGACAACGTTGTGGCC
+TGACTAGGTTCGGGCT
+TGACTAGTCACATACG
+TGACTTTCATTGGCGC
+TGAGAGGCATGTCGAT
+TGAGCCGAGCGAAGGG
+TGAGCCGCAGTCACTA
+TGAGGGAAGAGACTTA
+TGAGGGACATCCAACA
+TGCACCTCATGCAATC
+TGCCAAACAAACCTAC
+TGCCAAATCGAATCCA
+TGCCCTAAGTGTACTC
+TGCGCAGAGGAGTTTA
+TGCGCAGGTGGGTATG
+TGCGTGGGTCTCCACT
+TGCTACCCATGACGGA
+TGCTACCTCGGCTACG
+TGCTGCTGTTATTCTC
+TGCTGCTGTTCGGCAC
+TGCTGCTTCAAAGTAG
+TGGACGCAGACCACGA
+TGGACGCCAGAGTGTG
+TGGCGCACAGATGGGT
+TGGCTGGCACCCATTC
+TGGGAAGCATCCCATC
+TGGTTAGTCAACACAC
+TGGTTAGTCACAATGC
+TGGTTCCAGTATTGGA
+TGGTTCCCAAGCCGCT
+TGGTTCCCATCGTCGG
+TGTATTCGTAGCGTGA
+TGTCCCATCAACACTG
+TGTGGTAAGGGCATGT
+TTAACTCCAACACCTA
+TTAGGACCAGCCTTTC
+TTAGGACGTCTTTCAT
+TTAGTTCCAAGTAGTA
+TTATGCTCACCAACCG
+TTATGCTTCCACGTTC
+TTATGCTTCCCTTGTG
+TTCCCAGTCTTGCATT
+TTCGGTCAGCTAAACA
+TTCTCAAAGCTACCTA
+TTCTCAATCCACGTTC
+TTCTCAATCGCCTGAG
+TTCTCCTTCGCCAAAT
+TTCTTAGTCAGCTCGG
+TTGAACGAGCTTCGCG
+TTGAACGCACGAAAGC
+TTGCGTCGTTATTCTC
+TTGTAGGTCGGATGTT
+TTTACTGAGGCTAGGT
+TTTACTGCAGTGGAGT
+TTTCCTCCACTTGGAT
+TTTCCTCCATCTCGCT
+TTTGGTTAGCACCGTC
+TTTGGTTAGTGGGCTA
b
diff -r b9e04854e2bb -r 5ec75f5dae3c test-data/filtered3.Homo_sapiens.GRCh38.100.chr21.gtf
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/filtered3.Homo_sapiens.GRCh38.100.chr21.gtf Wed Aug 05 09:40:33 2020 -0400
b
b'@@ -0,0 +1,461 @@\n+#!genome-build GRCh38.p13\n+#!genome-version GRCh38\n+#!genome-date 2013-12\n+#!genome-build-accession NCBI:GCA_000001405.28\n+#!genebuild-last-updated 2019-06\n+21\thavana\tgene\t1000\t6346\t.\t+\t.\tgene_id "ENSG00000279493"; gene_version "1"; gene_name "FP565260.4"; gene_source "havana"; gene_biotype "protein_coding";\n+21\thavana\ttranscript\t1000\t6346\t.\t+\t.\tgene_id "ENSG00000279493"; gene_version "1"; transcript_id "ENST00000624081"; transcript_version "1"; gene_name "FP565260.4"; gene_source "havana"; gene_biotype "protein_coding"; transcript_name "FP565260.4-201"; transcript_source "havana"; transcript_biotype "protein_coding"; tag "cds_start_NF"; tag "mRNA_start_NF"; tag "basic"; transcript_support_level "5";\n+21\thavana\texon\t1000\t1075\t.\t+\t.\tgene_id "ENSG00000279493"; gene_version "1"; transcript_id "ENST00000624081"; transcript_version "1"; exon_number "1"; gene_name "FP565260.4"; gene_source "havana"; gene_biotype "protein_coding"; transcript_name "FP565260.4-201"; transcript_source "havana"; transcript_biotype "protein_coding"; exon_id "ENSE00003760288"; exon_version "1"; tag "cds_start_NF"; tag "mRNA_start_NF"; tag "basic"; transcript_support_level "5";\n+21\thavana\tCDS\t1000\t1075\t.\t+\t0\tgene_id "ENSG00000279493"; gene_version "1"; transcript_id "ENST00000624081"; transcript_version "1"; exon_number "1"; gene_name "FP565260.4"; gene_source "havana"; gene_biotype "protein_coding"; transcript_name "FP565260.4-201"; transcript_source "havana"; transcript_biotype "protein_coding"; protein_id "ENSP00000485664"; protein_version "1"; tag "cds_start_NF"; tag "mRNA_start_NF"; tag "basic"; transcript_support_level "5";\n+21\thavana\texon\t1749\t1888\t.\t+\t.\tgene_id "ENSG00000279493"; gene_version "1"; transcript_id "ENST00000624081"; transcript_version "1"; exon_number "2"; gene_name "FP565260.4"; gene_source "havana"; gene_biotype "protein_coding"; transcript_name "FP565260.4-201"; transcript_source "havana"; transcript_biotype "protein_coding"; exon_id "ENSE00003758404"; exon_version "1"; tag "cds_start_NF"; tag "mRNA_start_NF"; tag "basic"; transcript_support_level "5";\n+21\thavana\tCDS\t1749\t1888\t.\t+\t2\tgene_id "ENSG00000279493"; gene_version "1"; transcript_id "ENST00000624081"; transcript_version "1"; exon_number "2"; gene_name "FP565260.4"; gene_source "havana"; gene_biotype "protein_coding"; transcript_name "FP565260.4-201"; transcript_source "havana"; transcript_biotype "protein_coding"; protein_id "ENSP00000485664"; protein_version "1"; tag "cds_start_NF"; tag "mRNA_start_NF"; tag "basic"; transcript_support_level "5";\n+21\thavana\texon\t3587\t3672\t.\t+\t.\tgene_id "ENSG00000279493"; gene_version "1"; transcript_id "ENST00000624081"; transcript_version "1"; exon_number "3"; gene_name "FP565260.4"; gene_source "havana"; gene_biotype "protein_coding"; transcript_name "FP565260.4-201"; transcript_source "havana"; transcript_biotype "protein_coding"; exon_id "ENSE00003755466"; exon_version "1"; tag "cds_start_NF"; tag "mRNA_start_NF"; tag "basic"; transcript_support_level "5";\n+21\thavana\tCDS\t3587\t3672\t.\t+\t0\tgene_id "ENSG00000279493"; gene_version "1"; transcript_id "ENST00000624081"; transcript_version "1"; exon_number "3"; gene_name "FP565260.4"; gene_source "havana"; gene_biotype "protein_coding"; transcript_name "FP565260.4-201"; transcript_source "havana"; transcript_biotype "protein_coding"; protein_id "ENSP00000485664"; protein_version "1"; tag "cds_start_NF"; tag "mRNA_start_NF"; tag "basic"; transcript_support_level "5";\n+21\thavana\texon\t6136\t6346\t.\t+\t.\tgene_id "ENSG00000279493"; gene_version "1"; transcript_id "ENST00000624081"; transcript_version "1"; exon_number "4"; gene_name "FP565260.4"; gene_source "havana"; gene_biotype "protein_coding"; transcript_name "FP565260.4-201"; transcript_source "havana"; transcript_biotype "protein_coding"; exon_id "ENSE00003755385"; exon_version "1"; tag "cds_start_NF"; tag "mRNA_start_NF"; tag "basic"; transcript_support_level "5";\n+21\thavana\tCDS\t6136\t6298\t.\t+\t1\tgene_id "ENSG00000279493"; gene_'..b'\tgene\t586591\t586691\t.\t+\t.\tgene_id "ENSG00000277777"; gene_version "1"; gene_name "CU633967.2"; gene_source "ensembl"; gene_biotype "misc_RNA";\n+21\tensembl\ttranscript\t586591\t586691\t.\t+\t.\tgene_id "ENSG00000277777"; gene_version "1"; transcript_id "ENST00000610788"; transcript_version "1"; gene_name "CU633967.2"; gene_source "ensembl"; gene_biotype "misc_RNA"; transcript_name "CU633967.2-201"; transcript_source "ensembl"; transcript_biotype "misc_RNA"; tag "basic"; transcript_support_level "NA";\n+21\tensembl\texon\t586591\t586691\t.\t+\t.\tgene_id "ENSG00000277777"; gene_version "1"; transcript_id "ENST00000610788"; transcript_version "1"; exon_number "1"; gene_name "CU633967.2"; gene_source "ensembl"; gene_biotype "misc_RNA"; transcript_name "CU633967.2-201"; transcript_source "ensembl"; transcript_biotype "misc_RNA"; exon_id "ENSE00003748388"; exon_version "1"; tag "basic"; transcript_support_level "NA";\n+21\thavana\tgene\t692383\t694838\t.\t-\t.\tgene_id "ENSG00000279186"; gene_version "1"; gene_name "FP236315.2"; gene_source "havana"; gene_biotype "TEC";\n+21\thavana\ttranscript\t692383\t694838\t.\t-\t.\tgene_id "ENSG00000279186"; gene_version "1"; transcript_id "ENST00000624506"; transcript_version "1"; gene_name "FP236315.2"; gene_source "havana"; gene_biotype "TEC"; transcript_name "FP236315.2-201"; transcript_source "havana"; transcript_biotype "TEC"; tag "basic"; transcript_support_level "NA";\n+21\thavana\texon\t692383\t694838\t.\t-\t.\tgene_id "ENSG00000279186"; gene_version "1"; transcript_id "ENST00000624506"; transcript_version "1"; exon_number "1"; gene_name "FP236315.2"; gene_source "havana"; gene_biotype "TEC"; transcript_name "FP236315.2-201"; transcript_source "havana"; transcript_biotype "TEC"; exon_id "ENSE00003756739"; exon_version "1"; tag "basic"; transcript_support_level "NA";\n+21\thavana\tgene\t694546\t696361\t.\t+\t.\tgene_id "ENSG00000279784"; gene_version "1"; gene_name "FP236315.3"; gene_source "havana"; gene_biotype "lncRNA";\n+21\thavana\ttranscript\t694546\t696361\t.\t+\t.\tgene_id "ENSG00000279784"; gene_version "1"; transcript_id "ENST00000623587"; transcript_version "1"; gene_name "FP236315.3"; gene_source "havana"; gene_biotype "lncRNA"; transcript_name "FP236315.3-201"; transcript_source "havana"; transcript_biotype "lncRNA"; tag "basic"; transcript_support_level "2";\n+21\thavana\texon\t694546\t694654\t.\t+\t.\tgene_id "ENSG00000279784"; gene_version "1"; transcript_id "ENST00000623587"; transcript_version "1"; exon_number "1"; gene_name "FP236315.3"; gene_source "havana"; gene_biotype "lncRNA"; transcript_name "FP236315.3-201"; transcript_source "havana"; transcript_biotype "lncRNA"; exon_id "ENSE00003758868"; exon_version "1"; tag "basic"; transcript_support_level "2";\n+21\thavana\texon\t696164\t696361\t.\t+\t.\tgene_id "ENSG00000279784"; gene_version "1"; transcript_id "ENST00000623587"; transcript_version "1"; exon_number "2"; gene_name "FP236315.3"; gene_source "havana"; gene_biotype "lncRNA"; transcript_name "FP236315.3-201"; transcript_source "havana"; transcript_biotype "lncRNA"; exon_id "ENSE00003757033"; exon_version "1"; tag "basic"; transcript_support_level "2";\n+21\thavana\tgene\t696205\t698657\t.\t-\t.\tgene_id "ENSG00000279064"; gene_version "1"; gene_name "FP236315.1"; gene_source "havana"; gene_biotype "lncRNA";\n+21\thavana\ttranscript\t696205\t698657\t.\t-\t.\tgene_id "ENSG00000279064"; gene_version "1"; transcript_id "ENST00000623723"; transcript_version "1"; gene_name "FP236315.1"; gene_source "havana"; gene_biotype "lncRNA"; transcript_name "FP236315.1-201"; transcript_source "havana"; transcript_biotype "lncRNA"; tag "basic"; transcript_support_level "2";\n+21\thavana\texon\t696205\t696358\t.\t-\t.\tgene_id "ENSG00000279064"; gene_version "1"; transcript_id "ENST00000623723"; transcript_version "1"; exon_number "2"; gene_name "FP236315.1"; gene_source "havana"; gene_biotype "lncRNA"; transcript_name "FP236315.1-201"; transcript_source "havana"; transcript_biotype "lncRNA"; exon_id "ENSE00003756092"; exon_version "1"; tag "basic"; transcript_support_level "2";\n'
b
diff -r b9e04854e2bb -r 5ec75f5dae3c test-data/filtered3.Homo_sapiens.GRCh38.dna.chromosome.21.fa.gz
b
Binary file test-data/filtered3.Homo_sapiens.GRCh38.dna.chromosome.21.fa.gz has changed
b
diff -r b9e04854e2bb -r 5ec75f5dae3c test-data/filtered3.bam
b
Binary file test-data/filtered3.bam has changed
b
diff -r b9e04854e2bb -r 5ec75f5dae3c test-data/pbmc_1k_v2_L001.R1.10k.fastq.gz
b
Binary file test-data/pbmc_1k_v2_L001.R1.10k.fastq.gz has changed
b
diff -r b9e04854e2bb -r 5ec75f5dae3c test-data/pbmc_1k_v2_L001.R2.10k.fastq.gz
b
Binary file test-data/pbmc_1k_v2_L001.R2.10k.fastq.gz has changed
b
diff -r b9e04854e2bb -r 5ec75f5dae3c test-data/rnastar_index2_versioned.loc
--- a/test-data/rnastar_index2_versioned.loc Thu Nov 28 15:56:07 2019 -0500
+++ /dev/null Thu Jan 01 00:00:00 1970 +0000
b
@@ -1,4 +0,0 @@
-##STAR versioned indexes
-#build_id dbkey display_name path with_gene_model version
-000 ? test_index ${__HERE__}/test-cache/tophat-test 0 2.7.1a
-001 ? test_index_with_gene_model ${__HERE__}/test-cache/tophat-test 1 2.7.1a
b
diff -r b9e04854e2bb -r 5ec75f5dae3c test-data/rnastar_index2x_versioned.loc
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/rnastar_index2x_versioned.loc Wed Aug 05 09:40:33 2020 -0400
b
@@ -0,0 +1,4 @@
+##STAR versioned indexes
+#build_id dbkey display_name path with_gene_model version
+000 ? test_index ${__HERE__}/test-cache/tophat-test 0 2.7.4a
+001 ? test_index_with_gene_model ${__HERE__}/test-cache/tophat-test 1 2.7.4a
b
diff -r b9e04854e2bb -r 5ec75f5dae3c test-data/test-cache/tophat-test/Log.out
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/test-cache/tophat-test/Log.out Wed Aug 05 09:40:33 2020 -0400
b
@@ -0,0 +1,50 @@
+STAR version=2.7.5a
+STAR compilation time,server,dir=Tue Jun 16 12:17:16 EDT 2020 vega:/home/dobin/data/STAR/STARcode/STAR.master/source
+##### Command Line:
+STAR --runMode genomeGenerate --genomeDir tempstargenomedir --genomeFastaFiles tools/rgrnastar/test-data/tophat_test.fa --genomeSAindexNbases 5
+##### Initial USER parameters from Command Line:
+###### All USER parameters from Command Line:
+runMode                       genomeGenerate     ~RE-DEFINED
+genomeDir                     tempstargenomedir     ~RE-DEFINED
+genomeFastaFiles              tools/rgrnastar/test-data/tophat_test.fa        ~RE-DEFINED
+genomeSAindexNbases           5     ~RE-DEFINED
+##### Finished reading parameters from all sources
+
+##### Final user re-defined parameters-----------------:
+runMode                           genomeGenerate
+genomeDir                         tempstargenomedir
+genomeFastaFiles                  tools/rgrnastar/test-data/tophat_test.fa   
+genomeSAindexNbases               5
+
+-------------------------------
+##### Final effective command line:
+STAR   --runMode genomeGenerate   --genomeDir tempstargenomedir   --genomeFastaFiles tools/rgrnastar/test-data/tophat_test.fa      --genomeSAindexNbases 5
+----------------------------------------
+
+Number of fastq files for each mate = 1
+Finished loading and checking parameters
+--genomeDir directory created: tempstargenomedir/
+Jul 29 17:04:16 ... starting to generate Genome files
+tools/rgrnastar/test-data/tophat_test.fa : chr # 0  "test_chromosome" chrStart: 0
+Estimated genome size=201262144 = 262144 + 201000000
+GstrandBit=32
+Number of SA indices: 1300
+Jul 29 17:04:16 ... starting to sort Suffix Array. This may take a long time...
+Number of chunks: 1;   chunks size limit: 18599685304 bytes
+Jul 29 17:04:16 ... sorting Suffix Array chunks and saving them to disk...
+Writing 10400 bytes into tempstargenomedir//SA_0 ; empty space on disk = 66320359424 bytes ... done
+Jul 29 17:04:16 ... loading chunks from disk, packing SA...
+Jul 29 17:04:16 ... finished generating suffix array
+Jul 29 17:04:16 ... generating Suffix Array index
+Jul 29 17:04:16 ... completed Suffix Array index
+Jul 29 17:04:16 ... writing Genome to disk ...
+Writing 262144 bytes into tempstargenomedir//Genome ; empty space on disk = 66320355328 bytes ... done
+SA size in bytes: 5366
+Jul 29 17:04:16 ... writing Suffix Array to disk ...
+Writing 5366 bytes into tempstargenomedir//SA ; empty space on disk = 66320093184 bytes ... done
+Jul 29 17:04:16 ... writing SAindex to disk
+Writing 8 bytes into tempstargenomedir//SAindex ; empty space on disk = 66320084992 bytes ... done
+Writing 48 bytes into tempstargenomedir//SAindex ; empty space on disk = 66320084992 bytes ... done
+Writing 5971 bytes into tempstargenomedir//SAindex ; empty space on disk = 66320084992 bytes ... done
+Jul 29 17:04:16 ..... finished successfully
+DONE: Genome generation, EXITING
b
diff -r b9e04854e2bb -r 5ec75f5dae3c test-data/test-cache/tophat-test/genomeParameters.txt
--- a/test-data/test-cache/tophat-test/genomeParameters.txt Thu Nov 28 15:56:07 2019 -0500
+++ b/test-data/test-cache/tophat-test/genomeParameters.txt Wed Aug 05 09:40:33 2020 -0400
b
@@ -1,10 +1,13 @@
-### STAR   --runMode genomeGenerate   --genomeDir test-cache/tophat_test/   --genomeFastaFiles tophat_test.fa      --genomeSAindexNbases 5
+### STAR   --runMode genomeGenerate   --genomeDir tempstargenomedir   --genomeFastaFiles tools/rgrnastar/test-data/tophat_test.fa      --genomeSAindexNbases 5
 ### GstrandBit 32
-versionGenome 2.7.1a
-genomeFastaFiles tophat_test.fa 
+versionGenome 2.7.4a
+genomeType Full
+genomeFastaFiles tools/rgrnastar/test-data/tophat_test.fa 
 genomeSAindexNbases 5
 genomeChrBinNbits 18
 genomeSAsparseD 1
+genomeTransformType None
+genomeTransformVCF -
 sjdbOverhang 0
 sjdbFileChrStartEnd - 
 sjdbGTFfile -
b
diff -r b9e04854e2bb -r 5ec75f5dae3c tool-data/rnastar_index2_versioned.loc.sample
--- a/tool-data/rnastar_index2_versioned.loc.sample Thu Nov 28 15:56:07 2019 -0500
+++ /dev/null Thu Jan 01 00:00:00 1970 +0000
b
@@ -1,26 +0,0 @@
-#This is a sample file distributed with Galaxy that enables tools
-#to use a directory of rna-star indexed sequences data files. You will
-#need to create these data files and then create a rnastar_index2.loc
-#file similar to this one (store it in this directory) that points to
-#the directories in which those files are stored. The rnastar_index2.loc
-#file has this format (longer white space characters are TAB characters):
-#
-#<unique_build_id>   <dbkey>   <display_name>   <file_base_path> <with_gene_model> <version>
-#
-#The <with_gene_model> column should be 1 or 0, indicating whether the index
-#was built with annotations (i.e., --sjdbGTFfile and --sjdbOverhang were used)
-#or not.
-#
-#The <version> column indicates the STAR version that introduced the format of
-#the index, i.e., the oldest STAR version that could make use of the index.
-#
-#Note that STAR indices can become quite large. Consequently, it is only
-#advisable to create indices with annotations if it's known ahead of time that
-#(A) the annotations won't be frequently updated and (B) the read lengths used
-#will also rarely vary. If either of these is not the case, it's advisable to
-#create indices without annotations and then specify an annotation file and
-#maximum read length (minus 1) when running STAR.
-#
-#hg19   hg19    hg19 full   /mnt/galaxyIndices/genomes/hg19/rnastar 0 2.7.1a
-#hg19Ensembl   hg19Ensembl    hg19 full with Ensembl annotation   /mnt/galaxyIndices/genomes/hg19Ensembl/rnastar 1 2.7.1a
-
b
diff -r b9e04854e2bb -r 5ec75f5dae3c tool-data/rnastar_index2x_versioned.loc.sample
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/tool-data/rnastar_index2x_versioned.loc.sample Wed Aug 05 09:40:33 2020 -0400
b
@@ -0,0 +1,27 @@
+#This is a sample file distributed with Galaxy that enables tools
+#to use a directory of rna-star indexed sequences data files.
+#You will need to create these data files and then create a
+#rnastar_index2x_versioned.loc file similar to this one (store it in this
+directory) that points to the directories in which those files are stored.
+#The rnastar_index2x_versioned.loc file has this format (longer white space
+#characters are TAB characters):
+#
+#<unique_build_id>   <dbkey>   <display_name>   <file_base_path> <with_gene_model> <version>
+#
+#The <with_gene_model> column should be 1 or 0, indicating whether the index
+#was built with annotations (i.e., --sjdbGTFfile and --sjdbOverhang were used)
+#or not.
+#
+#The <version> column indicates the STAR version that introduced the format of
+#the index, i.e., the oldest STAR version that could make use of the index.
+#
+#Note that STAR indices can become quite large. Consequently, it is only
+#advisable to create indices with annotations if it's known ahead of time that
+#(A) the annotations won't be frequently updated and (B) the read lengths used
+#will also rarely vary. If either of these is not the case, it's advisable to
+#create indices without annotations and then specify an annotation file and
+#maximum read length (minus 1) when running STAR.
+#
+#hg19   hg19    hg19 full   /mnt/galaxyIndices/genomes/hg19/rnastar 0 2.7.1a
+#hg19Ensembl   hg19Ensembl    hg19 full with Ensembl annotation   /mnt/galaxyIndices/genomes/hg19Ensembl/rnastar 1 2.7.1a
+
b
diff -r b9e04854e2bb -r 5ec75f5dae3c tool_data_table_conf.xml.sample
--- a/tool_data_table_conf.xml.sample Thu Nov 28 15:56:07 2019 -0500
+++ b/tool_data_table_conf.xml.sample Wed Aug 05 09:40:33 2020 -0400
b
@@ -1,6 +1,6 @@
 <tables>
-    <table name="rnastar_index2_versioned" comment_char="#" allow_duplicate_entries="False">
+    <table name="rnastar_index2x_versioned" comment_char="#" allow_duplicate_entries="False">
         <columns>value, dbkey, name, path, with_gene_model, version</columns>
-        <file path="tool-data/rnastar_index2_versioned.loc" />
+        <file path="tool-data/rnastar_index2x_versioned.loc" />
     </table>
 </tables>
b
diff -r b9e04854e2bb -r 5ec75f5dae3c tool_data_table_conf.xml.test
--- a/tool_data_table_conf.xml.test Thu Nov 28 15:56:07 2019 -0500
+++ b/tool_data_table_conf.xml.test Wed Aug 05 09:40:33 2020 -0400
b
@@ -1,7 +1,7 @@
 <tables>
     <!-- Location of rnastar indexes for testing -->
-    <table name="rnastar_index2_versioned" comment_char="#" allow_duplicate_entries="False">
+    <table name="rnastar_index2x_versioned" comment_char="#" allow_duplicate_entries="False">
         <columns>value, dbkey, name, path, with_gene_model, version</columns>
-        <file path="${__HERE__}/test-data/rnastar_index2_versioned.loc" />
+        <file path="${__HERE__}/test-data/rnastar_index2x_versioned.loc" />
     </table>
 </tables>