| Previous changeset 29:b3d2d0a771e1 (2019-06-07) |
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README.md |
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| diff -r b3d2d0a771e1 -r 6669fd407dc9 README.md --- a/README.md Fri Jun 07 11:06:29 2019 -0400 +++ b/README.md Fri Jun 07 11:07:05 2019 -0400 |
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| @@ -1,7 +1,6 @@ This is the repository associated with the VAPPER Galaxy Tool available in the Galaxy Toolshed VAPP accurately quantifies the variant antigen diversity in Trypanosoma congolense isolates or the variable Cog presence in T.vivax isolates - The Trypanosoma congolense variant antigen repertoire is divided into 15 clades or phylotypes. These phylotypes are present in any T. congolense isolate, but their relative abundance varies between strains. The purpose of the VAPPER is to accurately quantify antigen diversity in any T. congolense isolate by calculating the relative frequency of each phylotype. The Galaxy VAPPER Tool has four modes. @@ -15,10 +14,11 @@ 3) T.vivax clusters of orthologs The approach for T. vivax relies on the presence/absence of clusters of orthologs (COGs). It requires velvet for the genome assembly and blast. it recieves paired sequencing reads in fastq format (or a contig file if already assembled) and the output is a binary matrix of the presence/absence of each COG/gene for a given sample. Within the tool there is a database of 28 isolates that are used as a comparison producing a heatmap and dendogram. -4) T.vivax Transcriptomic pathway -This method takes two NGS paired reads, maps the transcripts against a user supplied reference file and then estimates the trancript aboundance (using samtools and cufflinks). The output is then binned into 175 predefined phylotypes based on their greatest blastx similarity (this table is available in the results directory). A pident of less than 70% is considered unidentified. The results provide a tabular and graphical phylotype profile of the sample(s) +4) T.vivax Transcriptomic VAP +This method takes two NGS paired reads, maps the transcripts against a user-supplied reference file and +then estimates the trancript aboundance (using samtools and cufflinks). The output is then binned into +175 predefined phylotypes based on their greatest blastx similarity (this table is available in the results). A pident of less than 70% is considered unidentified. +The results are shown as tabular and graphical phylotype profiles. The user-supplied reference file should contain assembled transcripts. We advise it to be created using Trinity (Grabherr et al. 2013). - - |