Repository 'sfold'
hg clone https://toolshed.g2.bx.psu.edu/repos/iuc/sfold

Changeset 0:6cce721f568c (2024-11-27)
Commit message:
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sfold commit 41456127d154f251438945210c2513601bd21ceb
added:
macros.xml
sfold.xml
test-data/cons.ct
test-data/mfe.ct
test-data/seq.fasta
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diff -r 000000000000 -r 6cce721f568c macros.xml
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/macros.xml Wed Nov 27 13:22:29 2024 +0000
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@@ -0,0 +1,13 @@
+<macros>
+    <token name="@TOOL_VERSION@">2.2.0</token>
+    <token name="@VERSION_SUFFIX@">0</token>
+    <token name="@PROFILE@">23.0</token>
+    <xml name="requirements">
+        <requirement type="package" version="2.2">sfold</requirement>
+    </xml>
+    <xml name="citations">
+    <citations>        
+        <citation type="doi">10.1093/nar/gkh449</citation>
+    </citations>
+    </xml>
+</macros>
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diff -r 000000000000 -r 6cce721f568c sfold.xml
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/sfold.xml Wed Nov 27 13:22:29 2024 +0000
[
b'@@ -0,0 +1,335 @@\n+<tool id="sfold" name="Sfold" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@">\n+    <description>Prediction of  RNA secondary structures through structure ensemble sampling</description>\n+    <macros>\n+        <import>macros.xml</import>\n+    </macros>\n+    <requirements>\n+        <expand macro="requirements"/>\n+    </requirements>\n+    <command detect_errors="exit_code"><![CDATA[\n+        #if not $non_commercial_use\n+            >&2 echo "this tool is only available for non commercial use";\n+            exit 1;\n+        #end if\n+        mkdir -p \'output\' &&\n+        sfold \'$fasta\'\n+            -a $a\n+            #if $f: \n+                -f \'$f\'\n+            #end if\n+            #if str($l) != \'\':\n+                -l $l\n+            #end if\n+            #if $m: \n+                -m \'$m\'\n+            #end if\n+            -w $w\n+            -i $i\n+    ]]></command>\n+    <inputs>\n+        <param name="non_commercial_use" label="I certify that I am not using this tool for commercial purposes." type="boolean" truevalue="NON_COMMERCIAL_USE" falsevalue="COMMERCIAL_USE" checked="False">\n+            <validator type="expression" message="This tool is only available for non-commercial use.">value == True</validator>\n+        </param>\n+        <param argument="fasta" type="data" format="fasta" label="FASTA input"/>\n+        <param argument="-a" type="boolean" truevalue="1" falsevalue="0" checked="true" label="Run clustering on the sampled ensemble?"/>\n+        <param argument="-f" type="data" format="ct" optional="true" label="Constrain data" help="Constraint syntax follows what is used in mfold 3.1"/>\n+        <param argument="-l" type="integer" min="0" optional="true" label="Maximum distance between paired bases"/>\n+        <param argument="-m" type="data" format="ct" optional="true" label="MFE structure" help="If provided, Sfold clustering module will determine the cluster to which this structure belongs."/>\n+        <param argument="-w" type="integer" value="20" label="Length of antisense oligos"/>\n+        <param argument="-i" type="select" label="Module">\n+            <option value="0">None</option>\n+            <option value="1">Sirna</option>\n+            <option value="2">Soligo</option>\n+            <option value="3">Both</option>\n+        </param>\n+        <param name="output_selector" type="select" multiple="true" optional="true" display="checkboxes" label="Output options">\n+            <option value="ct" selected="true">Output ct files</option>\n+            <option value="bp" selected="true">Output bp files</option>\n+            <option value="clusters">Output cluster files</option>\n+        </param>\n+    </inputs>\n+    <outputs>\n+        <collection name="sfold_out" type="list" label="${tool.name} on ${on_string}: sfold log files">\n+            <discover_datasets pattern="(?P&lt;name&gt;.+)\\.out$" format="txt" directory="output/"/>\n+        </collection>\n+        <collection name="sfold_bp" type="list" label="${tool.name} on ${on_string}: sfold bp files">\n+            <discover_datasets pattern="(?P&lt;name&gt;.+)\\.bp$" format="txt" directory="output/"/>\n+            <filter>\'bp\' in output_selector</filter>\n+        </collection>\n+        <collection name="sfold_ct" type="list" label="${tool.name} on ${on_string}: sfold ct files">\n+            <discover_datasets pattern="(?P&lt;name&gt;.+)\\.ct$" format="ct" directory="output/"/>\n+            <filter>\'ct\' in output_selector</filter>\n+        </collection>\n+        <collection name="sfold_cluster_out" type="list" label="${tool.name} on ${on_string}: sfold cluster log files">\n+            <discover_datasets pattern="(?P&lt;name&gt;.+)\\.out$" format="txt" directory="output/clusters"/>\n+            <discover_datasets pattern="(?P&lt;name&gt;.+)\\.list$" format="txt" directory="output/clusters"/>\n+            <filter>\'clusters\' in output_selector</filter>\n+        </collection>\n+        <collection name="sfold_cluster_bp" type="list" label='..b'\n+                <element name="c01.ccentroid">\n+                    <assert_contents>\n+                        <has_text text="1 72"/>\n+                    </assert_contents>\n+                </element>\n+                <element name="c02.ccentroid">\n+                    <assert_contents>\n+                        <has_text text="Structure 1"/>\n+                    </assert_contents>\n+                </element>\n+            </output_collection>\n+            <output_collection name="sfold_cluster_ct" type="list">\n+                    <element name="c01.ccentroid">\n+                        <assert_contents>\n+                            <has_n_lines n="78"/>\n+                            <has_text text="Length:  76"/>\n+                        </assert_contents>\n+                    </element>\n+                    <element name="c02.ccentroid">\n+                        <assert_contents>\n+                            <has_n_lines n="78"/>\n+                            <has_text text="Length:  76"/>\n+                        </assert_contents>\n+                    </element>\n+            </output_collection>\n+        </test>\n+        <test expect_num_outputs="4">\n+            <param name="non_commercial_use" value="true"/>\n+            <param name="fasta" value="seq.fasta"/>\n+            <param name="a" value="true"/>\n+            <param name="f" value="cons.ct"/>\n+            <param name="l" value="30"/>\n+            <param name="m" value="mfe.ct"/>\n+            <param name="w" value="20"/>\n+            <param name="i" value="1"/>\n+            <param name="output_selector" value="bp,clusters"/>\n+            <output_collection name="sfold_cluster_bp" type="list">\n+                <element name="c01.ccentroid">\n+                    <assert_contents>\n+                        <has_text text="1 72"/>\n+                    </assert_contents>\n+                </element>\n+                <element name="c02.ccentroid">\n+                    <assert_contents>\n+                        <has_text text="Structure 1"/>\n+                        </assert_contents>\n+                </element>\n+            </output_collection>\n+            <output_collection name="sfold_bp" type="list">\n+                <element name="ecentroid">\n+                    <assert_contents>\n+                        <has_text text="1 72"/>\n+                    </assert_contents>\n+                </element>\n+            </output_collection>    \n+        </test>\n+    </tests>\n+    <help><![CDATA[\n+Sfold is a software package for statistical folding and rational design of nucleic acids.\n+\n+It provides tools for predicting RNA secondary structures, designing antisense oligonucleotides, and analyzing RNA folding patterns. Sfold is widely used in academic research for studying\n+RNA structure and function.\n+\n+Input Format\n+\n+-----------\n+\n+The program requires one input file of RNA sequence in FASTA format.\n+\n+Sfold options\n+\n+------------\n+\n+The program can run based on the clustering on the sampled ensemble or not.\n+It can also predict the secondary structure based on the constraint and MFE structure files.\n+The sfold has two different modules:\n+\n+* SiRNA : Target accessibility prediction and RNA duplex thermodynamics for rational siRNA design\n+* Solig: Target accessibility prediction and rational design of antisense oligonucleotides and nucleic acid probes\n+\n+The program can be run based on the above-mentioned modules, both of them or none of them.\n+\n+Output Files\n+\n+------------\n+\n+The Sfold program generates 4 different types of output files. sfold.out and sclass.out in your output log files, save the general messages from standard output/error from which you can know if your Sfold run was successful. Most of the other\n+files contain a header section that defines the formats and meanings of the data in the output.\n+\n+------------\n+\n+Important: The program usage is restricted to non-commercial and academic research.\n+    ]]>\n+    </help>\n+    <expand macro="citations" />\n+</tool>\n'
b
diff -r 000000000000 -r 6cce721f568c test-data/cons.ct
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/cons.ct Wed Nov 27 13:22:29 2024 +0000
[
@@ -0,0 +1,78 @@
+76 dG = -27.58 [Initially -28.90] test
+1 G 0 2 72 1
+2 G 1 3 71 2
+3 G 2 4 70 3
+4 G 3 5 69 4
+5 C 4 6 68 5
+6 U 5 7 67 6
+7 A 6 8 66 7
+8 U 7 9 0 8
+9 A 8 10 0 9
+10 G 9 11 25 10
+11 C 10 12 24 11
+12 U 11 13 23 12
+13 C 12 14 22 13
+14 A 13 15 0 14
+15 G 14 16 0 15
+16 C 15 17 0 16
+17 U 16 18 0 17
+18 G 17 19 0 18
+19 G 18 20 0 19
+20 G 19 21 0 20
+21 A 20 22 0 21
+22 G 21 23 13 22
+23 A 22 24 12 23
+24 G 23 25 11 24
+25 C 24 26 10 25
+26 G 25 27 0 26
+27 C 26 28 43 27
+28 U 27 29 42 28
+29 U 28 30 41 29
+30 G 29 31 40 30
+31 C 30 32 39 31
+32 A 31 33 38 32
+33 U 32 34 37 33
+34 G 33 35 0 34
+35 G 34 36 0 35
+36 C 35 37 0 36
+37 A 36 38 33 37
+38 U 37 39 32 38
+39 G 38 40 31 39
+40 C 39 41 30 40
+41 A 40 42 29 41
+42 A 41 43 28 42
+43 G 42 44 27 43
+44 A 43 45 0 44
+45 G 44 46 0 45
+46 G 45 47 0 46
+47 U 46 48 0 47
+48 C 47 49 0 48
+49 A 48 50 65 49
+50 G 49 51 64 50
+51 C 50 52 63 51
+52 G 51 53 62 52
+53 G 52 54 61 53
+54 U 53 55 0 54
+55 U 54 56 0 55
+56 C 55 57 0 56
+57 G 56 58 0 57
+58 A 57 59 0 58
+59 U 58 60 0 59
+60 C 59 61 0 60
+61 C 60 62 53 61
+62 C 61 63 52 62
+63 G 62 64 51 63
+64 C 63 65 50 64
+65 U 64 66 49 65
+66 U 65 67 7 66
+67 A 66 68 6 67
+68 G 67 69 5 68
+69 C 68 70 4 69
+70 U 69 71 3 70
+71 C 70 72 2 71
+72 C 71 73 1 72
+73 A 72 74 0 73
+74 C 73 75 0 74
+75 C 74 76 0 75
+76 A 75 0 0 76
+
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diff -r 000000000000 -r 6cce721f568c test-data/mfe.ct
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/mfe.ct Wed Nov 27 13:22:29 2024 +0000
[
@@ -0,0 +1,78 @@
+76 dG = -27.58 [Initially -28.90] test
+1 G 0 2 72 1
+2 G 1 3 71 2
+3 G 2 4 70 3
+4 G 3 5 69 4
+5 C 4 6 68 5
+6 U 5 7 67 6
+7 A 6 8 66 7
+8 U 7 9 0 8
+9 A 8 10 0 9
+10 G 9 11 25 10
+11 C 10 12 24 11
+12 U 11 13 23 12
+13 C 12 14 22 13
+14 A 13 15 0 14
+15 G 14 16 0 15
+16 C 15 17 0 16
+17 U 16 18 0 17
+18 G 17 19 0 18
+19 G 18 20 0 19
+20 G 19 21 0 20
+21 A 20 22 0 21
+22 G 21 23 13 22
+23 A 22 24 12 23
+24 G 23 25 11 24
+25 C 24 26 10 25
+26 G 25 27 0 26
+27 C 26 28 43 27
+28 U 27 29 42 28
+29 U 28 30 41 29
+30 G 29 31 40 30
+31 C 30 32 39 31
+32 A 31 33 38 32
+33 U 32 34 37 33
+34 G 33 35 0 34
+35 G 34 36 0 35
+36 C 35 37 0 36
+37 A 36 38 33 37
+38 U 37 39 32 38
+39 G 38 40 31 39
+40 C 39 41 30 40
+41 A 40 42 29 41
+42 A 41 43 28 42
+43 G 42 44 27 43
+44 A 43 45 0 44
+45 G 44 46 0 45
+46 G 45 47 0 46
+47 U 46 48 0 47
+48 C 47 49 0 48
+49 A 48 50 65 49
+50 G 49 51 64 50
+51 C 50 52 63 51
+52 G 51 53 62 52
+53 G 52 54 61 53
+54 U 53 55 0 54
+55 U 54 56 0 55
+56 C 55 57 0 56
+57 G 56 58 0 57
+58 A 57 59 0 58
+59 U 58 60 0 59
+60 C 59 61 0 60
+61 C 60 62 53 61
+62 C 61 63 52 62
+63 G 62 64 51 63
+64 C 63 65 50 64
+65 U 64 66 49 65
+66 U 65 67 7 66
+67 A 66 68 6 67
+68 G 67 69 5 68
+69 C 68 70 4 69
+70 U 69 71 3 70
+71 C 70 72 2 71
+72 C 71 73 1 72
+73 A 72 74 0 73
+74 C 73 75 0 74
+75 C 74 76 0 75
+76 A 75 0 0 76
+
b
diff -r 000000000000 -r 6cce721f568c test-data/seq.fasta
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/seq.fasta Wed Nov 27 13:22:29 2024 +0000
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@@ -0,0 +1,3 @@
+>gi|395400|emb|X66515|ECTRALA E.coli tRNA-Ala
+GGGGCTATAGCTCAGCTGGGAGAGCGCTTGCATGGCATGCAAGAGGTCAGCGGTTCGATC
+CCGCTTAGCTCCACCA   
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