Repository 'trinity_abundance_estimates_to_matrix'
hg clone https://toolshed.g2.bx.psu.edu/repos/iuc/trinity_abundance_estimates_to_matrix

Changeset 6:74217fbfba4d (2017-08-28)
Previous changeset 5:8b5039bdf1f6 (2017-03-31) Next changeset 7:34cefa17a764 (2017-12-14)
Commit message:
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit bc4ea54f0deec4ddec8e6cf79fd547491e165686
modified:
abundance_estimates_to_matrix.xml
added:
test-data/count/kallisto/abundance.tsv
test-data/count/kallisto/abundance.tsv.genes
test-data/count/kallisto/abundance_B.tsv
test-data/count/kallisto/abundance_B.tsv.genes
b
diff -r 8b5039bdf1f6 -r 74217fbfba4d abundance_estimates_to_matrix.xml
--- a/abundance_estimates_to_matrix.xml Fri Mar 31 11:37:24 2017 -0400
+++ b/abundance_estimates_to_matrix.xml Mon Aug 28 16:55:13 2017 -0400
[
@@ -1,4 +1,4 @@
-<tool id="trinity_abundance_estimates_to_matrix" name="Build expression matrix" version="@WRAPPER_VERSION@.0">
+<tool id="trinity_abundance_estimates_to_matrix" name="Build expression matrix" version="@WRAPPER_VERSION@.1">
     <description>for a de novo assembly of RNA-Seq data by Trinity</description>
     <macros>
         <import>macros.xml</import>
@@ -7,6 +7,7 @@
         <requirement type="package" version="3.16.5">bioconductor-edger</requirement>
         <!-- Cannot update to salmon 0.8.2 because in Bioconda it requires icu 58, while r-base 3.3.1 requires icu 54 -->
         <requirement type="package" version="0.8.1">salmon</requirement>
+        <requirement type="package" version="0.43.1">kallisto</requirement>
     </expand>
     <command detect_errors="aggressive"><![CDATA[
         #import re
@@ -30,6 +31,7 @@
             <option value="RSEM">RSEM</option>
             <option value="eXpress">eXpress</option>
             <option value="salmon">Salmon</option>
+            <option value="kallisto">Kallisto</option>
         </param>
 
         <section name="additional_params" title="Additional Options" expanded="False">
@@ -176,6 +178,29 @@
                 </assert_contents>
             </output>
         </test>
+        <test>
+            <param name="samples" ftype="tabular" value="count/kallisto/abundance.tsv.genes,count/kallisto/abundance_B.tsv.genes"/>
+            <param name="est_method" value="kallisto"/>
+            <param name="cross_sample_norm" value="TMM"/>
+            <output name="trans_counts">
+                <assert_contents>
+                    <has_line_matching expression="TRINITY_DN3_c0_g1&#009;.*" />
+                    <has_n_columns n="3" />
+                </assert_contents>
+            </output>
+            <output name="TPM_no_norm">
+                <assert_contents>
+                    <has_line_matching expression="TRINITY_DN3_c0_g1&#009;.*" />
+                    <has_n_columns n="3" />
+                </assert_contents>
+            </output>
+            <output name="norm">
+                <assert_contents>
+                    <has_line_matching expression="TRINITY_DN3_c0_g1&#009;.*" />
+                    <has_n_columns n="3" />
+                </assert_contents>
+            </output>
+        </test>
     </tests>
     <help>
 <![CDATA[
b
diff -r 8b5039bdf1f6 -r 74217fbfba4d test-data/count/kallisto/abundance.tsv
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/count/kallisto/abundance.tsv Mon Aug 28 16:55:13 2017 -0400
b
@@ -0,0 +1,8 @@
+target_id length eff_length est_counts tpm
+TRINITY_DN1_c0_g1_i1 380 133.091 13 114025
+TRINITY_DN0_c0_g1_i1 229 31.75 7 257370
+TRINITY_DN2_c0_g1_i1 279 55.7143 1 20952.6
+TRINITY_DN2_c1_g1_i1 541 279.667 15 62611.6
+TRINITY_DN2_c2_g1_i1 240 42.75 4 109227
+TRINITY_DN2_c3_g1_i1 202 19 0 0
+TRINITY_DN3_c0_g1_i1 216 18.75 7 435814
b
diff -r 8b5039bdf1f6 -r 74217fbfba4d test-data/count/kallisto/abundance.tsv.genes
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/count/kallisto/abundance.tsv.genes Mon Aug 28 16:55:13 2017 -0400
b
@@ -0,0 +1,8 @@
+target_id length eff_length est_counts tpm
+TRINITY_DN3_c0_g1 216.00 18.75 7.00 435814.00
+TRINITY_DN2_c2_g1 240.00 42.75 4.00 109227.00
+TRINITY_DN2_c3_g1 202.00 19.00 0.00 0.00
+TRINITY_DN0_c0_g1 229.00 31.75 7.00 257370.00
+TRINITY_DN2_c0_g1 279.00 55.71 1.00 20952.60
+TRINITY_DN1_c0_g1 380.00 133.09 13.00 114025.00
+TRINITY_DN2_c1_g1 541.00 279.67 15.00 62611.60
b
diff -r 8b5039bdf1f6 -r 74217fbfba4d test-data/count/kallisto/abundance_B.tsv
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/count/kallisto/abundance_B.tsv Mon Aug 28 16:55:13 2017 -0400
b
@@ -0,0 +1,8 @@
+target_id length eff_length est_counts tpm
+TRINITY_DN1_c0_g1_i1 229 31.75 7 257370
+TRINITY_DN0_c0_g1_i1 380 133.091 13 114025
+TRINITY_DN2_c0_g1_i1 279 55.7143 1 20952.6
+TRINITY_DN2_c2_g1_i1 541 279.667 15 62611.6
+TRINITY_DN2_c1_g1_i1 240 42.75 4 109227
+TRINITY_DN2_c3_g1_i1 202 19 0 0
+TRINITY_DN3_c0_g1_i1 216 18.75 7 435814
b
diff -r 8b5039bdf1f6 -r 74217fbfba4d test-data/count/kallisto/abundance_B.tsv.genes
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/count/kallisto/abundance_B.tsv.genes Mon Aug 28 16:55:13 2017 -0400
b
@@ -0,0 +1,8 @@
+target_id length eff_length est_counts tpm
+TRINITY_DN3_c0_g1 216.00 18.75 7.00 435814.00
+TRINITY_DN2_c1_g1 240.00 42.75 4.00 109227.00
+TRINITY_DN2_c3_g1 202.00 19.00 0.00 0.00
+TRINITY_DN0_c0_g1 380.00 133.09 13.00 114025.00
+TRINITY_DN2_c0_g1 279.00 55.71 1.00 20952.60
+TRINITY_DN1_c0_g1 229.00 31.75 7.00 257370.00
+TRINITY_DN2_c2_g1 541.00 279.67 15.00 62611.60