Repository 'bigwig_outlier_bed'
hg clone https://toolshed.g2.bx.psu.edu/repos/iuc/bigwig_outlier_bed

Changeset 1:8377a6abb4da (2024-07-21)
Previous changeset 0:ebcd48f183b3 (2024-07-05) Next changeset 2:61946b8bd43b (2024-07-25)
Commit message:
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bigwig_outlier_bed commit 92ffe42a6ad6e81f3f157bbc9b942c000d450416
modified:
bigwig_outlier_bed.py
bigwig_outlier_bed.xml
test-data/bedouthi2_sample
test-data/bedouthi_sample
test-data/bedouthilo2_sample
test-data/bedouthilo_sample
test-data/bedoutlo2_sample
test-data/bedoutlo_sample
test-data/table2_sample
test-data/table_sample
added:
test-data/bedouthi_qlo_notset_sample
test-data/bedouthilo_qlo_notset_sample
test-data/table3_sample
test-data/table_qlo_notset_sample
b
diff -r ebcd48f183b3 -r 8377a6abb4da bigwig_outlier_bed.py
--- a/bigwig_outlier_bed.py Fri Jul 05 06:00:15 2024 +0000
+++ b/bigwig_outlier_bed.py Sun Jul 21 11:03:36 2024 +0000
[
@@ -28,8 +28,6 @@
         self.bwnames = args.bigwig
         self.bwlabels = args.bigwiglabels
         self.bedwin = args.minwin
-        self.qlo = args.qlo
-        self.qhi = args.qhi
         self.outbeds = args.outbeds
         self.bedouthi = args.bedouthi
         self.bedoutlo = args.bedoutlo
@@ -37,7 +35,13 @@
         self.tableoutfile = args.tableoutfile
         self.bedwin = args.minwin
         self.qhi = args.qhi
-        self.qlo = args.qlo
+        self.qlo = None
+        try:
+            f = float(args.qlo)
+            self.qlo = f
+        except Exception as e:
+            s = str(e)
+            print(s, ' qlo=', args.qlo)
         nbw = len(args.bigwig)
         nlab = len(args.bigwiglabels)
         if nlab < nbw:
@@ -90,13 +94,13 @@
             bwmax,
         )
         if self.qhi is not None:
-            row += "\t%f" % self.bwtop
+            row += "\t%.2f" % self.bwtop
         else:
-            row += "\t"
+            row += "\tnoqhi"
         if self.qlo is not None:
-            row += "\t%f" % self.bwbot
+            row += "\t%.2f" % self.bwbot
         else:
-            row += "\t"
+            row += "\tnoqlo"
         return row
 
     def makeBed(self):
@@ -125,13 +129,31 @@
                     bwhi = self.processVals(bw, isTop=True)
                     for j, seg in enumerate(bwhi):
                         if seg[1] - seg[0] >= self.bedwin:
-                            bedhi.append((chr, seg[0], seg[1], "%s_hi" % (bwlabel), 1))
+                            score = np.sum(bw[seg[0]:seg[1]])
+                            bedhi.append(
+                                (
+                                    chr,
+                                    seg[0],
+                                    seg[1],
+                                    "%s_%d" % (bwlabel, score),
+                                    score,
+                                )
+                            )
                 if self.qlo is not None:
                     self.bwbot = np.quantile(bw, self.qlo)
                     bwlo = self.processVals(bw, isTop=False)
                     for j, seg in enumerate(bwlo):
                         if seg[1] - seg[0] >= self.bedwin:
-                            bedlo.append((chr, seg[0], seg[1], "%s_lo" % (bwlabel), -1))
+                            score = -1 * np.sum(bw[seg[0]:seg[1]])
+                            bedlo.append(
+                                (
+                                    chr,
+                                    seg[0],
+                                    seg[1],
+                                    "%s_%d" % (bwlabel, score),
+                                    score,
+                                )
+                            )
                 if self.tableoutfile:
                     row = self.makeTableRow(bw, bwlabel, chr)
                     restab.append(copy.copy(row))
@@ -164,7 +186,7 @@
     parser = argparse.ArgumentParser()
     a = parser.add_argument
     a("-m", "--minwin", default=10, type=int)
-    a("-l", "--qlo", default=None, type=float)
+    a("-l", "--qlo", default=None)
     a("-i", "--qhi", default=None, type=float)
     a("--bedouthi", default=None)
     a("--bedoutlo", default=None)
b
diff -r ebcd48f183b3 -r 8377a6abb4da bigwig_outlier_bed.xml
--- a/bigwig_outlier_bed.xml Fri Jul 05 06:00:15 2024 +0000
+++ b/bigwig_outlier_bed.xml Sun Jul 21 11:03:36 2024 +0000
b
@@ -1,5 +1,10 @@
-<tool name="Bigwig extremes to bed features" id="bigwig_outlier_bed" version="0.1.4" profile="22.05">
+<tool name="Bigwig extremes to bed features" id="bigwig_outlier_bed" version="@TOOL_VERSION@" profile="22.05">
   <description>Writes high and low bigwig runs as features in a bed file</description>
+  <macros>
+  <token name="@TOOL_VERSION@">0.2.0</token>
+  <token name="@NUMPY_VERSION@">2.0.0</token>
+  <token name="@PYTHON_VERSION@">3.12.3</token>
+  </macros>
   <edam_topics>
       <edam_topic>topic_0157</edam_topic>
       <edam_topic>topic_0092</edam_topic>
@@ -11,9 +16,9 @@
     <xref type="bio.tools">bigtools</xref>
   </xrefs>
   <requirements>
-    <requirement type="package" version="3.12.3">python</requirement>
-    <requirement type="package" version="2.0.0">numpy</requirement>
-    <requirement type="package" version="0.1.4">pybigtools</requirement>
+    <requirement type="package" version="@PYTHON_VERSION@">python</requirement>
+    <requirement type="package" version="@NUMPY_VERSION@">numpy</requirement>
+    <requirement type="package" version="@TOOL_VERSION@">pybigtools</requirement>
   </requirements>
   <required_files>
       <include path="bigwig_outlier_bed.py"/>
@@ -102,15 +107,25 @@
       <param name="qlo" value="0.01"/>
       <param name="tableout" value="create"/>
     </test>
+    <test expect_num_outputs="2">
+      <output name="bedouthi" value="bedouthi_qlo_notset_sample" compare="diff" lines_diff="0"/>
+      <output name="tableoutfile" value="table_qlo_notset_sample" compare="diff" lines_diff="0"/>
+      <param name="outbeds" value="outhi"/>
+      <param name="bigwig" value="bigwig_sample"/>
+      <param name="minwin" value="10"/>
+      <param name="qhi" value="0.99"/>
+      <param name="qlo" value=""/>
+      <param name="tableout" value="create"/>
+    </test>
     <test expect_num_outputs="3">
       <output name="bedouthi" value="bedouthi_sample" compare="diff" lines_diff="0"/>
       <output name="bedoutlo" value="bedoutlo_sample" compare="diff" lines_diff="0"/>
-      <output name="tableoutfile" value="table_sample" compare="diff" lines_diff="0"/>
+      <output name="tableoutfile" value="table3_sample" compare="diff" lines_diff="0"/>
       <param name="outbeds" value="outlohi"/>
       <param name="bigwig" value="bigwig_sample"/>
-      <param name="minwin" value="10"/>
-      <param name="qhi" value="0.99"/>
-      <param name="qlo" value="0.01"/>
+      <param name="minwin" value="1"/>
+      <param name="qhi" value="0.9"/>
+      <param name="qlo" value="0.1"/>
       <param name="tableout" value="create"/>
     </test>
     <test expect_num_outputs="4">
@@ -120,9 +135,9 @@
       <output name="tableoutfile" value="table2_sample" compare="diff" lines_diff="0"/>
       <param name="outbeds" value="outall"/>
       <param name="bigwig" value="bigwig_sample,1.bigwig"/>
-      <param name="minwin" value="10"/>
-      <param name="qhi" value="0.99"/>
-      <param name="qlo" value="0.01"/>
+      <param name="minwin" value="1"/>
+      <param name="qhi" value="0.9"/>
+      <param name="qlo" value="0.1"/>
       <param name="tableout" value="create"/>
     </test>
   </tests>
b
diff -r ebcd48f183b3 -r 8377a6abb4da test-data/bedouthi2_sample
--- a/test-data/bedouthi2_sample Fri Jul 05 06:00:15 2024 +0000
+++ b/test-data/bedouthi2_sample Sun Jul 21 11:03:36 2024 +0000
b
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b
diff -r ebcd48f183b3 -r 8377a6abb4da test-data/bedouthi_qlo_notset_sample
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/bedouthi_qlo_notset_sample Sun Jul 21 11:03:36 2024 +0000
b
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b
diff -r ebcd48f183b3 -r 8377a6abb4da test-data/bedouthi_sample
--- a/test-data/bedouthi_sample Fri Jul 05 06:00:15 2024 +0000
+++ b/test-data/bedouthi_sample Sun Jul 21 11:03:36 2024 +0000
b
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b
diff -r ebcd48f183b3 -r 8377a6abb4da test-data/bedouthilo2_sample
--- a/test-data/bedouthilo2_sample Fri Jul 05 06:00:15 2024 +0000
+++ b/test-data/bedouthilo2_sample Sun Jul 21 11:03:36 2024 +0000
b
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b
diff -r ebcd48f183b3 -r 8377a6abb4da test-data/bedouthilo_qlo_notset_sample
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/bedouthilo_qlo_notset_sample Sun Jul 21 11:03:36 2024 +0000
b
@@ -0,0 +1,70 @@
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b
diff -r ebcd48f183b3 -r 8377a6abb4da test-data/bedouthilo_sample
--- a/test-data/bedouthilo_sample Fri Jul 05 06:00:15 2024 +0000
+++ b/test-data/bedouthilo_sample Sun Jul 21 11:03:36 2024 +0000
b
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b
diff -r ebcd48f183b3 -r 8377a6abb4da test-data/bedoutlo2_sample
--- a/test-data/bedoutlo2_sample Fri Jul 05 06:00:15 2024 +0000
+++ b/test-data/bedoutlo2_sample Sun Jul 21 11:03:36 2024 +0000
b
@@ -1,91 +1,110 @@
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+chr21 9415270 9415275 1.bigwig_0 0
+chr21 9415320 9415325 1.bigwig_0 0
+chr21 9415385 9415390 1.bigwig_0 0
+chr21 9415470 9415475 1.bigwig_0 0
+chr21 9415535 9415540 1.bigwig_0 0
+chr21 9415595 9415600 1.bigwig_0 0
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+chr21 9415710 9415715 1.bigwig_0 0
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+chr21 9415880 9415885 1.bigwig_0 0
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+chr21 9416095 9416100 1.bigwig_0 0
+chr21 9416130 9416135 1.bigwig_0 0
+chr21 9416150 9416155 1.bigwig_0 0
+chr21 9416165 9416175 1.bigwig_0 0
+chr21 9416185 48129895 1.bigwig_0 0
b
diff -r ebcd48f183b3 -r 8377a6abb4da test-data/bedoutlo_sample
--- a/test-data/bedoutlo_sample Fri Jul 05 06:00:15 2024 +0000
+++ b/test-data/bedoutlo_sample Sun Jul 21 11:03:36 2024 +0000
b
@@ -1,69 +1,2 @@
-Merlin 80 90 bigwig_sample_lo -1
-Merlin 430 440 bigwig_sample_lo -1
-Merlin 1230 1240 bigwig_sample_lo -1
-Merlin 2670 2680 bigwig_sample_lo -1
-Merlin 3740 3750 bigwig_sample_lo -1
-Merlin 4700 4710 bigwig_sample_lo -1
-Merlin 5870 5890 bigwig_sample_lo -1
-Merlin 6290 6300 bigwig_sample_lo -1
-Merlin 8860 8870 bigwig_sample_lo -1
-Merlin 10520 10530 bigwig_sample_lo -1
-Merlin 11190 11200 bigwig_sample_lo -1
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-Merlin 12660 12670 bigwig_sample_lo -1
-Merlin 14160 14170 bigwig_sample_lo -1
-Merlin 14750 14760 bigwig_sample_lo -1
-Merlin 16270 16280 bigwig_sample_lo -1
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-Merlin 18290 18300 bigwig_sample_lo -1
-Merlin 19080 19090 bigwig_sample_lo -1
-Merlin 19220 19230 bigwig_sample_lo -1
-Merlin 20240 20250 bigwig_sample_lo -1
-Merlin 21650 21660 bigwig_sample_lo -1
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-Merlin 29400 29410 bigwig_sample_lo -1
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-Merlin 53150 53160 bigwig_sample_lo -1
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-Merlin 56020 56030 bigwig_sample_lo -1
-Merlin 57220 57230 bigwig_sample_lo -1
-Merlin 61250 61260 bigwig_sample_lo -1
-Merlin 63190 63200 bigwig_sample_lo -1
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-Merlin 67150 67160 bigwig_sample_lo -1
-Merlin 68790 68800 bigwig_sample_lo -1
-Merlin 69660 69670 bigwig_sample_lo -1
+Merlin 0 9 bigwig_sample_0 0
+Merlin 70049 180929 bigwig_sample_0 0
b
diff -r ebcd48f183b3 -r 8377a6abb4da test-data/table2_sample
--- a/test-data/table2_sample Fri Jul 05 06:00:15 2024 +0000
+++ b/test-data/table2_sample Sun Jul 21 11:03:36 2024 +0000
b
@@ -1,3 +1,3 @@
 bigwig contig n mean std min max qtop qbot
-bigwig_sample Merlin 70040 16414.614934 9523.634628 13.000000 32767.000000 32387.000000 323.000000
-1.bigwig chr21 4995 33.203203 21.071159 0.000000 100.000000 80.000000 0.000000
+bigwig_sample Merlin 180929 6354.313736 9951.749936 0.000000 32767.000000 24359.00 0.00
+1.bigwig chr21 48129895 0.003446 0.400601 0.000000 100.000000 0.00 0.00
b
diff -r ebcd48f183b3 -r 8377a6abb4da test-data/table3_sample
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/table3_sample Sun Jul 21 11:03:36 2024 +0000
b
@@ -0,0 +1,2 @@
+bigwig contig n mean std min max qtop qbot
+bigwig_sample Merlin 180929 6354.313736 9951.749936 0.000000 32767.000000 24359.00 0.00
b
diff -r ebcd48f183b3 -r 8377a6abb4da test-data/table_qlo_notset_sample
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/table_qlo_notset_sample Sun Jul 21 11:03:36 2024 +0000
b
@@ -0,0 +1,2 @@
+bigwig contig n mean std min max qtop qbot
+bigwig_sample Merlin 180929 6354.313736 9951.749936 0.000000 32767.000000 31906.72 noqlo
b
diff -r ebcd48f183b3 -r 8377a6abb4da test-data/table_sample
--- a/test-data/table_sample Fri Jul 05 06:00:15 2024 +0000
+++ b/test-data/table_sample Sun Jul 21 11:03:36 2024 +0000
b
@@ -1,2 +1,2 @@
 bigwig contig n mean std min max qtop qbot
-bigwig_sample Merlin 70040 16414.614934 9523.634628 13.000000 32767.000000 32387.000000 323.000000
+bigwig_sample Merlin 180929 6354.313736 9951.749936 0.000000 32767.000000 31906.72 0.00