| Previous changeset 2:f355085dd2aa (2024-01-11) |
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Commit message:
planemo upload for repository https://github.com/mesocentre-clermont-auvergne/galaxy-tools/tree/master/tools/polypolish commit f3b5a8f978923c5bbf663b0a99025245458e6ee0 |
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modified:
macro.xml polypolish.xml test-data/polished.fasta |
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| diff -r f355085dd2aa -r 915637bd1d1a macro.xml --- a/macro.xml Thu Jan 11 16:44:56 2024 +0000 +++ b/macro.xml Tue Nov 12 09:56:40 2024 +0000 |
| [ |
| @@ -1,48 +1,43 @@ - <macros> - <token name="@TOOL_VERSION@">0.5.0</token> - <token name="@VERSION_SUFFIX@">2</token> - <token name="@PROFILE@">21.05</token> - - <token name="@THREADS@"><![CDATA[ + <token name="@TOOL_VERSION@">0.6.0</token> + <token name="@VERSION_SUFFIX@">0</token> + <token name="@PROFILE@">23.02</token> + <token name="@THREADS@"><![CDATA[ ##compute the number of ADDITIONAL threads to be used by samtools (-@) addthreads=\${GALAXY_SLOTS:-1} && (( addthreads-- )) && ]]></token> - - <xml name="version_command"> - <version_command><![CDATA[polypolish -V]]></version_command> - </xml> - <xml name="xrefs"> - <xrefs> - <xref type='bio.tools'>Polypolish</xref> - </xrefs> - </xml> - <xml name="requirements"> - <requirements> - <requirement type="package" version="@TOOL_VERSION@">polypolish</requirement> - <requirement type="package" version="1.19">samtools</requirement> - </requirements> - </xml> - <xml name="citations"> - <citations> - <citation type="doi">10.1371/journal.pcbi.1009802</citation> - </citations> - </xml> - <!-- Filter option of polypolish --> - <xml name="filter_option"> - <conditional name="insert_filter"> - <param name="filter_select" type="select" label="Filter by insert size ? [recommanded]" - help="Exclude some alignments based on their insert size. It reduce the number of excessive alignments, - particularly near the edges of repeat sequences"> - <option value="filter" selected="true">Filter by insert size</option> - <option value="non_filter"> No filtering step</option> - </param> - <when value="filter"> - <param argument="--low" type="float" min="0" value="0.1" label="Low percentile threshold" help="Select the lower value to remove [default: 0.1]"/> - <param argument="--high" type="float" min="0" value="99.9" label="High percentile threshold" help="Select the lower value to remove [default: 99.9]"/> + <xml name="version_command"> + <version_command><![CDATA[polypolish -V]]></version_command> + </xml> + <xml name="xrefs"> + <xrefs> + <xref type="bio.tools">Polypolish</xref> + </xrefs> + </xml> + <xml name="requirements"> + <requirements> + <requirement type="package" version="@TOOL_VERSION@">polypolish</requirement> + <requirement type="package" version="1.19.2">samtools</requirement> + </requirements> + </xml> + <xml name="citations"> + <citations> + <citation type="doi">10.1371/journal.pcbi.1009802</citation> + </citations> + </xml> + <!-- Filter option of polypolish --> + <xml name="filter_option"> + <conditional name="insert_filter"> + <param name="filter_select" type="select" label="Filter by insert size ? [recommanded]" help="Exclude some alignments based on their insert size. It reduce the number of excessive alignments, particularly near the edges of repeat sequences"> + <option value="filter" selected="true">Filter by insert size</option> + <option value="non_filter"> No filtering step</option> + </param> + <when value="filter"> + <param argument="--low" type="float" min="0" value="0.1" label="Low percentile threshold" help="Select the lower value to remove [default: 0.1]"/> + <param argument="--high" type="float" min="0" value="99.9" label="High percentile threshold" help="Select the lower value to remove [default: 99.9]"/> + </when> + <when value="non_filter"> </when> - <when value="non_filter"> - </when> - </conditional> - </xml> + </conditional> + </xml> </macros> |
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| diff -r f355085dd2aa -r 915637bd1d1a polypolish.xml --- a/polypolish.xml Thu Jan 11 16:44:56 2024 +0000 +++ b/polypolish.xml Tue Nov 12 09:56:40 2024 +0000 |
| [ |
| b'@@ -5,9 +5,9 @@\n <macros>\n <import>macro.xml</import>\n </macros>\n- <expand macro=\'xrefs\'/>\n- <expand macro="requirements" />\n- <expand macro="version_command" />\n+ <expand macro="xrefs"/>\n+ <expand macro="requirements"/>\n+ <expand macro="version_command"/>\n <command detect_errors="aggressive"><![CDATA[\n ln -s \'$input.fasta_file\' input_data &&\n #*======================================\n@@ -21,7 +21,7 @@\n #else\n echo "${input.sam_data_type.single_sam} not a sam/bam file"\n #end if\n- polypolish input_data input_sam > \'$polished_fasta\'\n+ polypolish polish input_data input_sam > \'$polished_fasta\'\n #*======================================\n For paired SAM\n ======================================*#\n@@ -41,15 +41,15 @@\n echo "${input.sam_data_type.single_sam} not a sam/bam file"\n #end if\n #if $input.sam_data_type.insert_filter.filter_select == \'filter\'\n- polypolish_insert_filter.py --low \'$input.sam_data_type.insert_filter.low\'\n+ polypolish filter --low \'$input.sam_data_type.insert_filter.low\'\n --high \'$input.sam_data_type.insert_filter.high\'\n --in1 sample_R1.sam\n --in2 sample_R2.sam\n --out1 \'filtered_1.sam\'\n --out2 \'filtered_2.sam\' &&\n- polypolish input_data \'filtered_1.sam\' \'filtered_2.sam\' > $polished_fasta\n+ polypolish polish input_data \'filtered_1.sam\' \'filtered_2.sam\' > $polished_fasta\n #else\n- polypolish input_data sample_R1.sam sample_R2.sam > $polished_fasta\n+ polypolish polish input_data sample_R1.sam sample_R2.sam > $polished_fasta\n #end if\n #*======================================\n For multiple single-end SAM\n@@ -65,7 +65,7 @@\n echo "${single_sam} is not a sam/bam file"\n #end if\n #end for\n- polypolish input_data single_collection/*.sam > \'$polished_fasta\'\n+ polypolish polish input_data single_collection/*.sam > \'$polished_fasta\'\n #*======================================\n For multiple paired-end SAM\n ======================================*#\n@@ -83,7 +83,7 @@\n ln -s \'$paired_sam.reverse\' \'paired_collection/reverse_input$(value)$(paired_sam.reverse.element_identifier).sam\' &&\n #end if\n #if $input.sam_data_type.insert_filter.filter_select == \'filter\'\n- polypolish_insert_filter.py --low \'$input.sam_data_type.insert_filter.low\'\n+ polypolish filter --low \'$input.sam_data_type.insert_filter.low\'\n --high \'$input.sam_data_type.insert_filter.high\'\n --in1 \'paired_collection/forward_input$(value)$(paired_sam.forward.element_identifier).sam\'\n --in2 \'paired_collection/reverse_input$(value)$(paired_sam.reverse.element_identifier).sam\'\n@@ -95,9 +95,9 @@\n Filtering option\n ======================================*#\n #if $input.sam_data_type.insert_filter.filter_select == \'filter\'\n- polypolish input_data paired_collection/*_filtered.sam > \'$polished_fasta\'\n+ polypolish polish input_data paired_collection/*_filtered.sam > \'$polished_fasta\'\n #else\n- polypolish input_data paired_collection/*.sam > \'$polished_fasta\'\n+ polypolish polish input_data paired_collection/*.sam > \'$polished_fasta\'\n #end if\n #end if\n #*===================================='..b'an" truevalue="true" falsevalue="false" label="Keep per base information file"/>\n </section>\n@@ -369,29 +364,29 @@\n <section name="input">\n <param name="fasta_file" value="contigs.fa"/>\n <conditional name="sam_data_type">\n- <param name="sam_selector" value="multiple_paired"/>\n- <param name="paired_collection">\n- <collection type="list:paired">\n- <element name="paired_1">\n- <collection type="paired">\n- <element name="forward" value="aligned_test_file/alignement_R1.bam" ftype="unsorted.bam"/>\n- <element name="reverse" value="aligned_test_file/alignement_R2.bam" ftype="unsorted.bam"/>\n- </collection>\n- </element>\n- <element name="paired_2">\n- <collection type="paired">\n- <element name="forward" value="aligned_test_file/alignement_R1_bis.bam" ftype="unsorted.bam"/>\n- <element name="reverse" value="aligned_test_file/alignement_R2_bis.bam" ftype="unsorted.bam"/>\n- </collection>\n- </element>\n- <element name="paired_3">\n- <collection type="paired">\n- <element name="forward" value="aligned_test_file/alignement_R1_ter.bam" ftype="unsorted.bam"/>\n- <element name="reverse" value="aligned_test_file/alignement_R2_ter.bam" ftype="unsorted.bam"/>\n- </collection>\n- </element>\n- </collection>\n- </param>\n+ <param name="sam_selector" value="multiple_paired"/>\n+ <param name="paired_collection">\n+ <collection type="list:paired">\n+ <element name="paired_1">\n+ <collection type="paired">\n+ <element name="forward" value="aligned_test_file/alignement_R1.bam" ftype="unsorted.bam"/>\n+ <element name="reverse" value="aligned_test_file/alignement_R2.bam" ftype="unsorted.bam"/>\n+ </collection>\n+ </element>\n+ <element name="paired_2">\n+ <collection type="paired">\n+ <element name="forward" value="aligned_test_file/alignement_R1_bis.bam" ftype="unsorted.bam"/>\n+ <element name="reverse" value="aligned_test_file/alignement_R2_bis.bam" ftype="unsorted.bam"/>\n+ </collection>\n+ </element>\n+ <element name="paired_3">\n+ <collection type="paired">\n+ <element name="forward" value="aligned_test_file/alignement_R1_ter.bam" ftype="unsorted.bam"/>\n+ <element name="reverse" value="aligned_test_file/alignement_R2_ter.bam" ftype="unsorted.bam"/>\n+ </collection>\n+ </element>\n+ </collection>\n+ </param>\n </conditional>\n </section>\n <section name="options">\n@@ -429,7 +424,7 @@\n <output name="debug_file" value="debug_file_test_2.tsv"/>\n </test>\n </tests>\n- <help><![CDATA[\n+ <help><![CDATA[\n **What it does**\n Polypolish is a tool for polishing genome assemblies with short reads.\n Polypolish uses SAM/BAM files where each read has been aligned to all possible locations (not just a single best location).\n' |
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| diff -r f355085dd2aa -r 915637bd1d1a test-data/polished.fasta --- a/test-data/polished.fasta Thu Jan 11 16:44:56 2024 +0000 +++ b/test-data/polished.fasta Tue Nov 12 09:56:40 2024 +0000 |
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| b'@@ -1,8 +1,8 @@\n->contig00001_polypolish\n+>contig00001 len=5513 cov=11.7 corr=0 origname=NODE_1_length_5513_cov_11.741181_pilon sw=shovill-spades/1.1.0 date=20220302 polypolish\n CAGCATCGGACTCAGATAGTAATCCACGCTCTTTTAAAATGTCAACAAGAGAATCTCTACCATGAACAAAATGTGACTCATATCTAAACCAGTCCTTGACGAACGTGCCAAGCATATTAAGCCACTTCTCCTCATCCAACGCGTCAGTTTTTGACAGAATCGTTAGTTGATGGCGAAAGGTCGCAAAGTAAGAGCTTCTCGAGCTGCGCAAGGATAGGTCGAATTTTCTCATTTTCCGCCAGCAGTCCACTTCGATTTAATTCGTAAACAAGCAGTAGTAATTCCTGCTTTATCAAGATAATTTTTCGACTCATCAGAAATATCCGAAAGTGTTAACTTCTGCGTCATGGAAGCGATAAAACTCTGCAGGTTGGATACGCCAATCATTTTTATCGAAGCGCGCATAAATTTGAGCAGATTTGTCGTCACAGGTTGCGCCGCCAAAACGTCGGCTACAGTAACTTTTCCCAGCCTCAATCTCATCTCTCTTTTTGCGTTCTGCTTCAATATCTGGTTGAACGGCGTCGCGTCGTAACCCAGCTTGGTAAGTTGGATTAAGCACTCCGTGGACAGATTTGTCATTGTGAGCATTTTCATCCCGAAGTTGCGGCTCATTCTGATTCTGAACAGCTTCTTGGGAAGTAGCGACAGCTTGGTTTTTAGTGAGTTGTTCCATTCTTTAGCTCCTAGACCTTTAGCAGCAAGGTCCATATCTGACTTTTTGTTAACGTATTTAGCCACATAGAAACCAACAGCCATATAACTGGTAGCTTTAAGCGGCTCACCTTTAGCATCAACAGGCCACAACCAACCAGAACGTGAAAAAGCGTCCTGCGTGTAGCGAACTGCGATGGGCATACTGTAACCATAAGGCCACGTATTTTGCAAGCTATTTAACTGGCGGCGATTGCGTACCCGACGACCAAAATTAGGGTCAACGCTACCTGTAGGAAGTGTCCGCATAAAGTGCACCGCATGGAAATGAAGACGGCCATTAGCTGTACCATACTCAGGCACACAAAAATACTGATAGCAGTCGGCGTGTGAATCATTAGCCTTGCGACCCTCGGCAGCAAGAACCATACGACCAATATCACGAAAATAGTCACGCAAAGCATTGGGATTATCATAAAACGCCTCTAATCGGTCGTCAGCCAACGTGAGAGTGTCAAAAACGATAAACCAACCATCAGCATGAGCCTGTCGCATTGCATTCATCAAACGCTGAATAGCAAAGCCTCTACGCGATTTCATAGTGGAGGCCTCCAGCAATCTTGAACACTCATCCTTAATACCTTTCTTTTTGGGGTAATTATACTCATCGCGAATATCCTTAAGAGGGCGTTCAGCAGCCAGCTTGCGGCAAAACTGCGTAACCGTCTTCTCGTTCTCTAAAAACCATTTTTCGTCCCCTTCGGGGCGGTGGTCTATAGTGTTATTAATATCAAGTTGGGGGAGCACATTGTAGCATTGTGCCAATTCATCCATTAACTTCTCAGTAACAGATACAAACTCATCACGAACGTCAGAAGCAGCCTTATGGCCGTCAACATACATATCACCATTATCGAACTCAACGCCCTGCATACGAAAAGACAGAATCTCTTCCAAGAGCTTGATGCGGTTATCCATCTGCTTATGGAAGCCAAGCATTGGGGATTGAGAAAGAGTAGAAATGCCACAAGCCTCAATAGCAGGTTTAAGAGCCTCGATACGCTCAAAGTCAAAATAATCAGCGTGACATTCAGAAGGGTAATAAGAACGAACCATAAAAAAGCCTCCAAGATTTGGAGGCATGAAAACATACAATTGGGAGGGTGTCAATCCTGACGGTTATTTCCTAGACAAATTAGAGCCAATACCATCAGCTTTACCGTCTTTCCAGAAATTGTTCCAAGTATCGGCAACAGCTTTATCAATACCATGAAAAATATCAACCACACCAGAAGCAGCATCAGTGACGACATTAGAAATATCCTTTGCAGTAGCGCCAATATGAGAAGAGCCATACCGCTGATTCTGCGTTTGCTGATGAACTAAGTCAACCTCAGCACTAACCTTGCGAGTCATTTCTTTGATTTGGTCATTGGTAAAATACTGACCAGCCGTTTGAGCTTGAGTAAGCATTTGGCGCATAATCTCGGAAACCTGCTGTTGCTTGGAAAGATTGGTGTTTTCCATAATAGACGCAACGCGAGCAGTAGACTCCTTCTGTTGATAAGCAAGCATCTCATTTTGTGCATATACCTGGTCTTTCGTATTCTGGCGTGAAGTCGCCGACTGAATGCCAGCAATCTCTTTTTGAGTCTCATTTTGCATCTCGGCAATCTCTTTCTGATTGTCCAGTTGCATTTTAGTAAGCTCTTTTTGATTCTCAAATCCGGCGTCAACCATACCAGCAGAGGAAGCATCAGCACCAGCACGCTCCCAAGCATTAAGCTCAGGAAATGCAGCAGCAAGATAATCACGAGTATCCTTTCCTTTATCAGCGGCAGACTTGCCACCAAGTCCAACCAAATCAAGCAACTTATCAGAAACGGCAGAAGTGCCAGCCTGCAACGTACCTTCAAGAAGTCCTTTACCAGCTTTAGCCATAGCACCAGAAACAAAACTAGGGACGGCCTCATCAGGGTTAGGAACATTAGAGCCTTGAATGGCAGATTTAATACCAGCATCACCCATGCCTACAGTATTGTTATCGGTAGCAAGCACATCACCTTGAATGCCACCGGAGGCGGCTTTTTGACCGCCTCCAAACAATTTAGACATGGCGCCACCAGCAAGAGCAGAAGCAATACCGCCAGCAATAGCACCAAACATAAATCACCTCACTTAAGTGGCTGGAGACAAATAATCTCTTTAATAACCTGATTCAGCGAAACCAATCCGCGGCATTTAGTAGCGGTAAAGTTAGACCAAACCATGAAACCAACATAAACATTATTGCCCGGCGTACGGGGAAGGACGTCAATAGTCACACAGTCCTTGACGGTATAATAACCACCATCATGGCGACCATCCAAAGGATAAACATCATAGGCAGTCGGGAGGGTAGTCGGAACCGAAGAAGACTCAAAGCGAACCAAACAGGCAAAAAATTTAGGGTCGGCATCAAAAGCAATATCAGCACCAACAGAAACAACCTGATTAGCGGCGTTGACAGATGTATCCATCTGAATGCAATGAAGAAAACCACCATTACCAGCATTAACCGTCAAACTATCAAAATATAACGTTGACGATGTAGCTTTAGGTGTCTGTAAAACAGGTGCCGAAGAAGCTGGAGTAACAGAAGTGAGAACCAGCTTATCAGAAAAAAAGTTTGAATTATGGCGAGAAATAAAAGTCTGAAACATGATTAAACTCCTAAGCAGAAAACCTACCGCGCTTCGCTTGGTCAACCCCTCAGCGGCAAAAATTAAAATTTTTACCGCTTCGGCGTTATAACCTCACACTCAATCTTTTATCACGAAGTCATGATTGAATCGCGAGTGGTCGGCAGATTGCGATAAACGGTCACATTAAATTTAACCTGACTATTCCACTGCAACAACTGAACGGACTGGAAACACTGGTCATAATCATGGTGGCGAATAAGTACGCGTTCTTGCAAATCACCAGAAGGCGGTTCCTGAATGAATGGGAAGCCTTCAAGAAGGTGATAAGCAGGAGAAACATACGAAGGCGCATAACGATACCACTGACCCTCAGCAATCTTAAACTTCTTAGACGAATCACCAGAACGGAAAACATCCTTCATAGAAATTTCACGCGGCGGCAAGTTGCCATACAAAACAGGGTCGCCAGCAATATCGGTATAA'..b'TGATGCAGCTACTTACTATGGAAATGGTCTTTATTGTAACAAAGAAAAATGTTGGGTAGATTGGAATCAAGCTAAAGGAGAAATTGGAAAAATTATTGTTAATGGTTGGGTTAATCATGGTCCATGGGCACCTAGAAGGTAGTGGTAGGGAGGTTATTATAATGGATTTTACTAAAGAAGAAAAACTTTTAAATGCAATTAGTAAAGTATACAATGAAGCAACTATAGATGACTATCCTGACTTAAAAGAAAAGCTCTTTCTTTATTCTAAAGAAATCAGTGAGGGAAAAAGTGTTGGTGAAGTTAGTATGAAATTAAGTAGTTTTCTTGGAAGATATATTTTAAAACATAAATTTGGATTACCTAAATCTTTAATAGAATTACAAGAAATTGTTAGTAAGGAATCTCAAGTATATAGAGGATGGGCTTCTATTGGTATTTGGAGTTAATTCTTTGGAATAAAATGAAAATGGGACTGAAGAATCAATTCTGATTCATCAGTCCCATAAATTATAGAGCCGAAAAATTATCATATTTATAGATTTATTAATAGTAAATGGCATAAGAATCTTTGAACAACGAAAAAAATGTAGCTCTACCTAATTATTGATTTGCAACAATTTCACCGAAGGTGAAACAAGCGAAACTTTACAAAAAGTGGCTAGCATTTGCTAGTCTTTTTTGTATTGTGGAGAGTGG\n->contig00004_polypolish\n+>contig00004 len=3138 cov=2.3 corr=1 origname=NODE_4_length_3138_cov_2.261375_pilon sw=shovill-spades/1.1.0 date=20220302 polypolish\n TTTTTTTCCGATATAATTTGCTGGTAAATCTATCACAGCCAATGAGAGTCCGGGATTGCTTTCTTCCTGATAATCTTTCCGTTGGTATAAAATCTTTTCGCCTAGATATACAGTAATCCATTGATGGTTTCCTTTGATTAGTAAATTTGCTTCGTCCAACGGTTTTTCAAGTGTCGTTTCCATAATGAATGTTTCGTCTTTATCCACGGTAGGAATCGTACGAGTGTACTTGCTGTCAAACTTAAAATCAGGATCATCTTTTGTATAGAAAACCCACGAATCTTTTATTTCTTTCATTTGATTGCTGTCCGCAGGAGAAAAAATAAAATAAAAGAAATAAGCAATTCCTGTTAAAAATAACAATGATAATATAATGATTATGCTCCACTTTATACGTAAATAAAAAGATTCTTTTTTATTATATATGTTATTAAAAAAAGACATAAAGGTATTCCTTTGAGAGTATAAAATATTTTGTGTAAATGAAAAAATCCATACAAAAAAGGAAGTCCCTTCTGTAGAATAAAGTTAACGACAACCAATTCACAGAAAAGAGGACTTCCCTATGAATGATTTTACTACAGAAATTGTGCAAACTCTAGTCACTAAAGGCGATTTAAATGAATTATTCCGTTCGCACTTAGAAAAAGCGATAAACACACTCCTACGGACTGAATTAACGGCTTTTTTAGATTACGAAAAATATGATCGCACTGGTTTTAATTCAGGTAATTCGAGAAACGGTTCTTACTTTCGATCAATCAAAACCGAATATGGTGAATTAACATTGGAAATACCTAGAGATCGTAATGGTGAGTTTAAACAACAAACTTTACCAGCCTACAAAAGAACAAACGATACATTGGAAACCACTATTATCCATTTATTCGAAAAAGGTGTTACGATGTCTGAAATTGCTGATTTGATCGAAAAAATGTACGGTCATCACTATACTCCACAAACCATGTCCAACATGACTAAAGTTCTGACTGAAGAAGTAAATGCCTTTAAATCCAGAGCCTTAAATGATAAGTATGTCGCTATTTTTATGGACGTTACTTACATTCCACTAAAACGTCAAACTGTATCCAAAGAAGCGATTTATATTGCCATTGGTATACGAGAAGACGGCACTAAAGAAGTACTGAGTTATGCGATTGCTCCAACTGAATCAACATACGTTTGGAATGAGCTGCTACAGGATATTAACTCCAGAGGAGTTCAAGAAGTCTTGCTTTTTATTACGGACGGCTTAAAAGGCATGAAAGATACTATCCATCAAATTTATCCTAAAGCAAAATATCAGCATTGTTGTATCCATGTATCTCGTAACATCGCTCATAAAGTACGTGTCAAAGACCGAAAAGAAATCTGTGATGACTTTAAGGCTGTTTATCAAGCTAACTCAAAAGAAGAAGCGAATACCTTCTTATCCGGCATGATTGAGAAATGGAAGAAAAACTATCCTAAAGTGACGCAGTCACTCATAGAAAACCAAGACTTATTAACTTTTTATGATTTTCCACCTAGCATTCGTAGAACCATTTACTCAACCAATCTAATCGAGTCTTTCAATAAGCAAATTAAAAGATACAGCCGTAGAAAAGAGCAGTTTCAAAATGAAGAATCACTAGAACGCTTTCTAGTCAGCATTTTTGATACATACAATCAAAAATTTCTAAACAGAAGCCATAAAGGTTTTCAACAGGTAACCGATACATTAGTTTCAATGTTTACTGAGTAACTAATTATTTTGCAGGAGGACAATTTATTTACACAAAATTATTGACGCTCCCTAACAAAATCCCACCTACTTTATCTTATAACTTGTTTTTTTGCATAAAAACTAAATAAAGAGTGCGGCATTTCGCACTTTTTTATTATATATTATTTGTCAAATTAAGCGAGACAAAACTTTCCATTTACGTAACTCAAAAAAACTTCTAATGGTGTTTGGTAATTTAGTGATTTTCTAGGGATATGGTTTCTTTTGGACGCAACGGATGAGATGAATCCTTGATTGACTTGGTGGAAGTCCATTTCTTTTGGTAGTCCATCTTTTCGGAGGAGTCCGTTTGAATGTTCATTTAATCCCCGTTGGGAAGGCGTTCCTGGGTCTGCGAAATAAATATCAATATCATTCGTATTACTAATACTTTTCCAATTAGAGAATTCTTTTCCACAATCAAAGGTAATTGATTTGAAAAGATTTTTGGGTACGGATTGAAGCCATTCATTAATCGAATTTTCAATATCAACTGCCTTACGGCCTTCTGGTTTCAAGGCGATAATGGCTTTTGAAAGTCGCTCAACGAGTGTGATGACGGCACTTTTATGGTGGATGCCGACAATCGTATCACCCTCTAAATGTCCAAATTCTTCTTCGAAAACGACATAATCTTTTTTACGTTCAGAAATATTTCTTTTGAATGCCTGTTTTCCACGTTTTTCCTTATAACCATTTGGTTTTCGTTTTCCTTGCATCGGTAAATGTAAAACATTGAATTCTCCCGTCTTAAACCGGCGATAGAGGGTACTTACTGAACAAGAGAAGGTTCGCTCTGCACGGCCAATAATGACGTCTGGGGTCCAACCTTCAGTTGATCTTTTTGCAATGTATTCTTTTTCCTCAGCAGGAAAAATGATTTCGGTTCTCCCACAACGTCGCTTATTTTCTTTGTATTGTTCAAAGTATTCAAGTGCTGTTCCACCACATTTGAGAAAGTTATAGACATTGTAAATTGTTTGGCGTCCACGTTTAAGCTGCTTCGCAACGATAGCAACCGGAGTTTCTTGATGAAAATATGCTTCTATCATTACAAGCTCGTTTGGTGTAAGATGGGTATAAGTCATTTATGTTCACTCTCCTTGTATGCTTTAGCGGGTATTACAATTGGAGTGTAACATAAATGGCTTTTTTATTTGTCTCGCTTAATTATACAAACGGCGTTGTTAAATAAATAGGAACAAAAAAATTAAATTATTTAATACACACATTAGGGATCATTCATTGAAGGATTAGAAGACAACGAGGATGGCGAAAAATATGATTACAATAGAAAAGTCGTTCTAAACCAATCAGTTCCTAGTATCACACAACGCAAGCCAGTCGATTTGGTGAGATTAGAGGAGTGAAAG\n' 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