| Next changeset 1:ba271365095e (2017-10-11) |
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Commit message:
planemo upload for repository https://github.com/phac-nml/bio_hansel commit c35a4502f95471e22dea2d77f36b92c47424cecc |
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added:
bio_hansel.xml test-data/SRR1002850_SMALL.fasta |
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| diff -r 000000000000 -r 9407a9eaad22 bio_hansel.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/bio_hansel.xml Wed Sep 27 11:50:41 2017 -0400 |
| [ |
| b'@@ -0,0 +1,252 @@\n+<tool id="bio_hansel" name="Hansel - Salmonella Subtyping" version="0.1.0">\n+ <requirements>\n+ <requirement type="package" version="0.1.0">bio_hansel</requirement>\n+ <requirement type="package" version="17.2.0">attrs</requirement>\n+ </requirements>\n+ <command detect_errors="exit_code"><![CDATA[\n+ ## Variables to be set.\n+ #set outputResultsName = \'results.tab\'\n+ #set outputMatchResultsName = \'match_results.tab\'\n+ #set verboseDebuggingLevel = \'-vvv\'\n+\n+ ## Preparing file input.\n+ #if $single_or_paired.type == "paired":\n+\n+ ln -s \'$single_or_paired.fastq_input1\' fast_q_paired_1.fastq &&\n+ ln -s \'$single_or_paired.fastq_input2\' fast_q_paired_2.fastq &&\n+\n+ #elif $single_or_paired.type == "collection":\n+\n+ ln -s \'$single_or_paired.fastq_input1.forward\' fast_q_paired_1.fastq &&\n+ ln -s \'$single_or_paired.fastq_input1.reverse\' fast_q_paired_2.fastq &&\n+\n+ #elif $single_or_paired.type == "single":\n+\n+ #if $single_or_paired.fastq_input1.is_of_type(\'fastqsanger\') or $single_or_paired.fastq_input1.is_of_type(\'fastq\'):\n+ ln -s \'$single_or_paired.fastq_input1\' fast_q_single.fastq &&\n+ #end if\n+\n+ #if $single_or_paired.fastq_input1.is_of_type(\'fasta\'):\n+ ln -s \'$single_or_paired.fastq_input1\' fast_a_single.fasta &&\n+ #end if\n+\n+ #end if\n+\n+\n+ ## Checking for custom scheme.\n+ #if $type_of_scheme.scheme_type == "custom":\n+ #if $type_of_scheme.scheme_input.is_of_type(\'fasta\'):\n+ ln -s \'$type_of_scheme.scheme_input\' custom_scheme.fasta &&\n+ #end if\n+ #end if\n+\n+\n+ ## Start the actual command here\n+ hansel \n+\n+\n+ ## Select the scheme\n+ -s\n+\n+ #if $type_of_scheme.scheme_type == "heidelberg":\n+ heidelberg\n+ #elif $type_of_scheme.scheme_type == "enteritidis":\n+ enteritidis\n+ #elif $type_of_scheme.scheme_type == "custom":\n+ custom_scheme.fasta\n+ #end if\n+\n+\n+ #if $kmer_min\n+ --min-kmer-freq $kmer_min\n+ #end if\n+\n+ #if $kmer_max\n+ --max-kmer-freq $kmer_max\n+ #end if\n+\n+ ## Adding more parameters to the command.\n+ $verboseDebuggingLevel -t "\\${GALAXY_SLOTS:-1}" -o $outputResultsName -O $outputMatchResultsName \n+\n+\n+ ## Entering the file inputs.\n+ #if $single_or_paired.type == "single":\n+\n+ #if $single_or_paired.fastq_input1.is_of_type(\'fastqsanger\') or $single_or_paired.fastq_input1.is_of_type(\'fastq\'):\n+ fast_q_single.fastq\n+ #end if\n+\n+ #if $single_or_paired.fastq_input1.is_of_type(\'fasta\'):\n+ fast_a_single.fasta\n+ #end if\n+\n+ #else\n+\n+ ##use -p to declare using two files.\n+ -p fast_q_paired_1.fastq fast_q_paired_2.fastq\n+\n+ #end if\n+ ]]></command>\n+ <inputs>\n+ <conditional name="single_or_paired">\n+ <param name="type" type="select" label="Specify the read type.">\n+ <option value="single">Single-end Data</option>\n+ <option value="paired">Paired-end Data</option>\n+ <option value="collection">Collection Paired-end Data</option>\n+ </param>\n+ <when value="single">\n+ <param name="fastq_input1" type="data" format="fastqsanger, fastq, fasta" label="Single end read file(s)"/>\n+ </when>\n+ <when value="paired">\n+ <param name="fastq_input1" type="data" format="fastqsanger, fastq" label="Forward paired-end read file"/>\n+ <param name="fastq_input2" type="data" format="fastqsanger, fastq" label="Reverse paired-end read file"/>\n+ </when>\n+ <when value="collection">\n+ <param name="fastq_input1" type="data_collection" label='..b'tch gapopen qstart qend sstart send evalue bitscore qlen slen seq coverage is_trunc refposition subtype is_pos_tile sample file_path scheme\n+\n+ 775920-2.2.2.2 NODE_2_length_512016_cov_46.4737_ID_3 100.0 33 0 0 1 33 474875 474907 2.0000000000000002e-11 62.1 33 512016 GTTCAGGTGCTACCGAGGATCGTTTTTGGTGCG 1.0 False 775920 2.2.2.2 \n+\n+ True SRR1002850 SRR1002850.fasta heidelberg\n+\n+ negative3305400-2.1.1.1 NODE_3_length_427905_cov_48.1477_ID_5 100.0 33 0 0 1 33 276235 276267 2.0000000000000002e-11 62.1 33 427905 CATCGTGAAGCAGAACAGACGCGCATTCTTGCT 1.0 False \n+\n+ negative3305400 2.1.1.1 False SRR1002850 SRR1002850.fasta heidelberg\n+\n+ negative3200083-2.1 NODE_3_length_427905_cov_48.1477_ID_5 100.0 33 0 0 1 33 170918 170950 2.0000000000000002e-11 62.1 33 427905 ACCCGGTCTACCGCAAAATGGAAAGCGATATGC 1.0 False \n+\n+ negative3200083 2.1 False SRR1002850 SRR1002850.fasta heidelberg\n+\n+ negative3204925-2.2.3.1.5 NODE_3_length_427905_cov_48.1477_ID_5 100.0 33 0 0 1 33 175760 175792 2.0000000000000002e-11 62.1 33 427905 CTCGCTGGCAAGCAGTGCGGGTACTATCGGCGG 1.0 False \n+\n+ negative3204925 2.2.3.1.5 False SRR1002850 SRR1002850.fasta heidelberg\n+\n+ negative3230678-2.2.2.1.1.1 NODE_3_length_427905_cov_48.1477_ID_5 100.0 33 0 0 1 33 201513 201545 2.0000000000000002e-11 62.1 33 427905 AGCGGTGCGCCAAACCACCCGGAATGATGAGTG 1.0 False \n+\n+ negative3230678 2.2.2.1.1.1 False SRR1002850 SRR1002850.fasta heidelberg\n+\n+ negative3233869-2.1.1.1.1 NODE_3_length_427905_cov_48.1477_ID_5 100.0 33 0 0 1 33 204704 204736 2.0000000000000002e-11 62.1 33 427905 CAGCGCTGGTATGTGGCTGCACCATCGTCATTA 1.0 False \n+\n+ [Next 196 lines omitted.]\n+\n+\n+Analysis of a single FASTQ readset\n+----------------------------------\n+\n+Contents of ``results.tab``:\n+\n+ sample scheme subtype all_subtypes tiles_matching_subtype are_subtypes_consistent inconsistent_subtypes n_tiles_matching_all n_tiles_matching_all_total n_tiles_matching_positive n_tiles_matching_positive_total n_tiles_matching_subtype n_tiles_matching_subtype_total file_path\n+\n+ SRR5646583 heidelberg 2.2.1.1.1.1 2; 2.2; 2.2.1; 2.2.1.1; 2.2.1.1.1; 2.2.1.1.1.1 1983064-2.2.1.1.1.1; 4211912-2.2.1.1.1.1 True 202 202 20 20 2 2 SRR5646583_forward.fastqsanger; SRR5646583_reverse.fastqsanger\n+\n+\n+Contents of ``match_results.tab``:\n+\n+ seq freq sample file_path tilename is_pos_tile subtype refposition is_kmer_freq_okay scheme\n+\n+ ACGGTAAAAGAGGACTTGACTGGCGCGATTTGC 68 SRR5646583 SRR5646583_forward.fastqsanger; SRR5646583_reverse.fastqsanger 21097-2.2.1.1.1 True 2.2.1.1.1 21097 True heidelberg\n+\n+ AACCGGCGGTATTGGCTGCGGTAAAAGTACCGT 77 SRR5646583 SRR5646583_forward.fastqsanger; SRR5646583_reverse.fastqsanger 157792-2.2.1.1.1 True 2.2.1.1.1 157792 True heidelberg\n+\n+ CCGCTGCTTTCTGAAATCGCGCGTCGTTTCAAC 67 SRR5646583 SRR5646583_forward.fastqsanger; SRR5646583_reverse.fastqsanger 293728-2.2.1.1 True 2.2.1.1 293728 True heidelberg\n+\n+ GAATAACAGCAAAGTGATCATGATGCCGCTGGA 91 SRR5646583 SRR5646583_forward.fastqsanger; SRR5646583_reverse.fastqsanger 607438-2.2.1 True 2.2.1 607438 True heidelberg\n+\n+ CAGTTTTACATCCTGCGAAATGCGCAGCGTCAA 87 SRR5646583 SRR5646583_forward.fastqsanger; SRR5646583_reverse.fastqsanger 691203-2.2.1.1 True 2.2.1.1 691203 True heidelberg\n+\n+ CAGGAGAAAGGATGCCAGGGTCAACACGTAAAC 33 SRR5646583 SRR5646583_forward.fastqsanger; SRR5646583_reverse.fastqsanger 944885-2.2.1.1.1 True 2.2.1.1.1 944885 True heidelberg\n+\n+ [Next 200 lines omitted.]\n+ \n+\n+Wrapper written by Matthew Gopez at the Public Health Agency of Canada, National Microbiology Laboratory.\n+ ]]></help>\n+ <citations>\n+ </citations>\n+</tool>\n\\ No newline at end of file\n' |
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| diff -r 000000000000 -r 9407a9eaad22 test-data/SRR1002850_SMALL.fasta --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/SRR1002850_SMALL.fasta Wed Sep 27 11:50:41 2017 -0400 |
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| b'@@ -0,0 +1,766 @@\n+>NODE_384_length_56_cov_34125_ID_767\n+CTAGCCTGATTCAGTTTTATCTTTCGTCGGTGAGCGACGGTACTTTCTGACCTCGG\n+>NODE_383_length_56_cov_335_ID_765\n+CAATTGTGATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGCCCCGAAAA\n+>NODE_382_length_56_cov_201_ID_763\n+CCGTCGCTCACCGACGAAAGATAAAACGGAATCAGGCTAGCGGGGTGTCGGGGTGC\n+>NODE_377_length_57_cov_34097.5_ID_753\n+AAACCGAGGTCAGAAAGTACCGTCGCTCACCGACGAAAGATAAAACTGAATCAGGCT\n+>NODE_376_length_57_cov_200_ID_751\n+GAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAGTGCCGCCAGGCAAATTCTTT\n+>NODE_375_length_58_cov_19568.3_ID_749\n+TAATACCGTCACACGCGACGCTATCACAATTGCCATCTGGTCAGGGGTGCACCCCGAC\n+>NODE_374_length_58_cov_29262.3_ID_747\n+GCACCCCGACACCCCGCTAGCCTGATTCAGTTTTATCTTTCGTCGGTGAGCGACGGTA\n+>NODE_372_length_59_cov_291.25_ID_743\n+TAGCCTGATTCAGTTTTATCTTTCGTCGGTGAGCGACGGGACTTTCTGACCTCGGTTTT\n+>NODE_369_length_60_cov_34761.2_ID_737\n+GCGGTGGCCGAAACCCTGAAATATTCCACGAAGCCGGCTGACATGGTGGCCGATCCTGAA\n+>NODE_367_length_60_cov_167.2_ID_733\n+CCGAGGGCGACGATGACGGTTCACGAATCGCGTTCGAGTGGAAACCCGAGGTCAGAAAGT\n+>NODE_365_length_61_cov_228.5_ID_729\n+TCGCGTTCGAGTGGAAAACCGAGGTCAGAAAGTCCCGTCGCTCACCGACGAAAGATAAAAC\n+>NODE_360_length_62_cov_66.7143_ID_719\n+CAGGGGTGCACCCCGACACCCCGCTAGCCTGATTCAGTTTTATCTTTCGTCGGTGGGCGACG\n+>NODE_357_length_63_cov_202.375_ID_713\n+GGGGTGTGGGGGTGCACCCCTGACCAGATGGCAATTGTGATAGCGTCGCGTGTGACGGTATTA\n+>NODE_356_length_63_cov_142.75_ID_711\n+TGTAATTGTAATAACGTCACACGCGACGCTATCACAATTGCCATCTGGTCAGGGGGGCACCCC\n+>NODE_355_length_64_cov_127.667_ID_709\n+CGTCGCTCCCCGACGAAAGATAAAACTGAATCAGGCTAGCGGGGTGTCGGGGTGCCCCCCTGAC\n+>NODE_352_length_64_cov_30.6667_ID_703\n+CGTCGCTCCCCGACGAAAGATAAAACTGAATCAGGCTAGGGGGGTGTCGGGGTGCACCCCTGAC\n+>NODE_351_length_64_cov_104.444_ID_701\n+ATGATATTTCCGAGGGCGACGATGACGGTTCACGAATCGCGTTCGAGTGGAAACCCGAGGTCAG\n+>NODE_349_length_64_cov_29.3333_ID_697\n+GTCAGGGGGGCACCCCGACACCCCCCTAGCCTGATTCAGTTTTATCTTTCGTCGGGGAGCGACG\n+>NODE_347_length_64_cov_32_ID_693\n+GTCAGGGGGGCACCCCGACACCCCGCTAGCCTGATTCAGTTTTTTCTTTCGTCGGGGAGCGACG\n+>NODE_346_length_65_cov_38729.2_ID_691\n+CTGACCTCGGTTTTCCACTCGAACGCGATTCGTGAACCGTCATCGTCGCCCTCGGAAATATCATC\n+>NODE_342_length_66_cov_28.1818_ID_683\n+CTGACCTCGGGTTTCCACTCGAACGCGATTCGTGAACCGTCATCGTCGCCCTCGGGAATATCATCA\n+>NODE_340_length_67_cov_71.3333_ID_679\n+CCGACGAAAGATAAAACTGAATCAGGCTAGGGGGGTGTCGGGGTGCCCCCCTGACCAGATGGCAATT\n+>NODE_336_length_67_cov_56_ID_671\n+CTTGAATATGGCGGTGAGGGGGGGATTGACTCGCTTTGCTCGCCCCTTCGGGGCAGCCCGTTCGCTG\n+>NODE_335_length_68_cov_32300.2_ID_669\n+CCCCGACACCCCGCTAGCCTGATTCAGTTTTATCTTTCGTCGGTGAGCGACGGTACTTTCTGACCTCG\n+>NODE_333_length_69_cov_126.429_ID_665\n+GGTGTCGGGGTGCCCCCCTGACCAGAGGGCAATTGTGATAGCGTCGCGTGTGACGGTATTACAATTACA\n+>NODE_332_length_69_cov_101.286_ID_663\n+GGTGTCGGGGTGCCCCCCTGACCAGATGGCATTTGTGATAGCGTCGCGTGTGACGGTATTACAATTACA\n+>NODE_327_length_71_cov_31.375_ID_653\n+GCACCCCGACACCCCCCTAGCCTGATTCAGTTTTATCTTTCGTCGGGGAGCGACGGTACTTTCTGACCTCG\n+>NODE_326_length_71_cov_108.125_ID_651\n+CCGAGGTCAGAAAGTCCCGTCGCTCCCCGACGAAAGATAAAACTGAATCAGGCTAGCGGGGTGTCGGGGTG\n+>NODE_324_length_71_cov_35.75_ID_647\n+TGTAATTGTAATACCCTCACACGCGACGCTATCACAATTGCCATCTGGTCAGGGGGGCACCCCGACACCCC\n+>NODE_318_length_74_cov_355.895_ID_635\n+GTAGGCGTTATTGGCGCAGCCAGTTTGGACACGGACAGCGCGCAAAAACCGGAGCGTACACGTAGTACGTGAGG\n+>NODE_316_length_76_cov_59.5714_ID_631\n+CGTCGCTCACCGACGAAAGAAAAAACTGAATCAGGCTAGGGGGGTGTCGGGGTGCACCCCTGACCAGATGGCAATT\n+>NODE_315_length_76_cov_76.8571_ID_629\n+CGTCGCTCACCGACGAAAGATAAAACTGAATCAGGCTAGCGGGGTGTGGGGGTGCACCCCTGACCAGATGGCAATT\n+>NODE_314_length_76_cov_172.333_ID_627\n+AATTGCCATCTGGTCAGGGGTGCACCCCGACACCCCGCTAGCCTGATTCCGTTTTATCTTTCGTCGGTGAGCGACG\n+>NODE_313_length_76_cov_35231.5_ID_625\n+AAACGGCAAAATGATTTACTCGCCGTTTGTGTGGGTTGTTTTGTGGGGTGTCAATGGGGTTGTGGTCTTTTTTGTG\n+>NODE_312_length_76_cov_28.9048_ID_623\n+CGTCGCTCACCGACGAAAGAAAAAACTGAATCAGGCTAGGGGGGTGTCGGGGTGCCCCCCTGACCAGATGGCAATT\n+>NODE_310_length_79_cov_105.5_ID_619\n+CTCAAAAAACCGAGAAACGGCGGGGATTTTCGGGACAGGATGTGTAATTGTAATAACGTCACACGCGACGCTATCACAA\n+>NODE_306_length_82_cov_64.2963_ID_611\n+TATCACAATTGCCATCTGGTCAGGGGGGCACCCCGACACCCCGCTAGCCTGATTCCGTTTTATCTTTCGTCGGTGAGCGACG\n+>NODE_294_length'..b'GGATGGCCGCGTCTTTGAAGCGCTACTGGTTGGCTCCGATTCGCTTACCGATCTGGCGGTGCTGAAGATCAACGCCACTGGCGGGCTGCCTACCATCCCGATTAATACAAAGCGTACACCGCATATTGGCGACGTCGTACTGGCTATCGGCAACCCATATAATCTGGGACAGACCATTACCCAGGGGATCATCAGCGCAACGGGTCGTATCGGCCTGAACCCGACGGGGCGACAGAATTTTCTCCAGACCGACGCCTCGATTAACCACGGTAATTCCGGCGGCGCGCTGGTCAACTCGTTAGGCGAACTGATGGGGATCAACACCCTCTCTTTTGATAAGAGTAACGATGGTGAAACGCCGGAAGGCCTTGGTTTTGCGATTCCCTTCCAGCTAGCCACGAAAATTATGGATAAGCTTATCCGCGACGGTCGTGTGATTCGCGGCTATATCGGTATTGGCGGACGAGAAATCGCGCCGCTGCACGCGCAGCAGGGTAGCGGCATGGACCCGATTCAGGGCATTGTCGTTAATGAAGTGACGCCAAACGGCCCCGCCGCGCTTGCCGGTATTCAGGTTAATGATTTGATTATTTCGGTCAATAATAAACCCGCCGTGTCCGCGCTGGAGACAATGGATCAGGTGGCGGAAATCCGCCCGGGCTCCGTCATTCCGGTCGTGGTAATGCGGGATGATAAGCAACTCACGTTCCAGGTGACGGTGCAGGAATACCCGGCGTCGAACTAAAACGACGCGGCTTTTGCCTGCGTCGTTCGACGGCTAGTCAATAAAGACTGGCCCTGCCGGAAAATGCGCCAGGAAACTCGTGAAGATCAGCAGAGCGGGGAGACTTTTCTCCCCGACGGCTTCTTCACGCAGCGCCTTATCCAGTGCAAGCAGGCTGTGCTCCCCCCCTTCCCCCAAAATGCCGCCCATCCAGAGCAGTCGTTGCGCCTGTTGACGCGCATACTGCGATGCGCTTTTCCAGGCGACCAGATGCAGCAACGTTTGTAACAGCAGGACCCGAGACGCGTGACCATCGCGTTGCAATCGCCTGAATACGGGTAATGCAATACTACGTACCGTCGAGAAACCGCTCGCCGCCTCCCCCTCAATCGGCGATAAACCAACAGCCGCCTGTTTGACGAAATCGCATAACTGATTGTGTGTTAATGGCCGCTCGCCTGCGCTCATATACCCTGCGGCAAAACAGAGTAACCCGATACCCTGAGCCCAATCGGTCGATGGCAGAATCCCCTGCGGAACATTCGGTGCCGACGTCTGTGCGGCACAAAGCCCCTCGTCGTAACACTGTGCAAAACGTTGCTCATATATTGCTGAGTTGATGCCAGACCACGGCAGTAAATGCAGCGCTGGCTCAAGCTCAGTTATCTGATTCACCGTCTCAACAACATAACGCAAGATGGTTTCTCTGGATGGCATGGATGTACCTTGCCGAAACGCAACCATTTCACTCATTACTGTGTCCTCATTTTGCGGGAGCAGAATCACTGCTCCCGATTAGATTGGATTACATTGCCAGCACGTACTTCAGCATCACGCCCGCCGCAATGGCCGAACCGATAACCCCCGCCACGTTCGGGCCCATCGCGTGCATCAGCAGGAAGTTCTGCGGGTCCGACTCCAGGCCCACCTTGTTCGATACGCGGGCCGCCATCGGCACCGCCGACACCCCCGCCGAACCGATAAGCGGGTTGATTTTGTTCTTACTGCACAGGTTCAGCAGCTTCGCCATCAGCACCCCGGCGGCAGTCCCGATACCAAAGGCAATCACACCCAGCAGCAGAATGCCCAGCGTCTGCGGCTGCAGGAACTTGTCCGCCACCAGCTTCGCCCCTACCGACAGCCCGAGGAAAATGGTGACGATGTTGATCAGCCCGTTCTGCACTGTGTCGCTCAGGCGCTCCACCACGCCGCTTTCACGCATCAGATTGCCGAAGCAGAACATCCCCAGCAGCGGCGCGGCGTCCGGCAGCAGCAGCGCCACCAGTAGTAACAGCACTACCGGGAAGAGGATTTTTTCCCGCTTACTCACCGTGCGCAGCTGCACCATGCGGATTTTCCGCTCCGTCTCCGTGGTCAGCGCGCGCATAATCGGCGGCTGGATTAACGGCACCAGCGCCATATACGAGTACGCCGCCACCGCGATGGCCCCCAGCAGCTCCGGCGCCAGTTTGCCCGACAGATAAATGGCCGTCGGACCGTCCGCGCCGCCGATGATGCCTATCGCCGCCGCCTGCGGCAGGGTGAAGGAAATCAGGCCGAAGTAATTCAGCGTCAGCGCCCCCAGCACCGTGGCGAAGATGCCGAACTGCGCCGCCGCGCCGAGCAGCAGGGTACGCGGGTTGGCCAGCAGCGGGCCGAAGTCGGTCATCGCGCCGACGCCCATAAAGATGACCAGCGGCGCGACGCCGGAGCCAATCGCCACTTTATAGAACAGCGCCAGCACCCCCGGCGTGTAGCCCATGTCCACCGCCAGGTTCTCCATCTGGCTCTGAACGGAGGGCAGCGCCAGCGCTAACGCCTCCTTGATGGCGTGCACGTCCGGCGCGCAGTTAAGCTTCGCGGCAATCACCGCCAGCTGCCCGGCGTCGTGGTGCGCCAGCAGGCTTTCCAGCGCGGTCAGCGCCATGCCCGCTTCCGGGATGTTGGAGAGCAGGCCGCCGAAGCCAATCGGCAACAGCAACAACGGCTCGAACTTTTTCGCAATCGCCAGCCACAGCAGCAGCAGGCTGACCAGCAGCATGATGGCCTGGCCTGCGCCAAGGTGCATCAGCCCCATGCCCTGAAGCAGGGCGTTCAGACTTTCCATTCGTCCTCTCCGTTACGCAAGCTGCATCAGGGTGTCGCCCACCGCCACCGCATCCCCGGCTTTCACCGCGATGCCGCGTACCGTCCCGGCCTGCGCGGCGCGGATTTCGGTTTCCATTTTCATGGCTTCCAGAATCAGCAGCACATCGCCTTCGGCCACCGTCTGGCCTTCCGTCGCCACCACTTTCCAGATATTGCCCGCCAGCGGCGCGCTGACCGGGGTGCCCGCGCCCGCCGGGGCGGCGGCAGGCGCCGCGGTCTGAACCGGCGCAGAACTGGCCGTGGCCAGCTGGCTGATATCGCCGCCGTCGCTGACTTTCACCACAAAGGCTTTGCCTTCCACTTCGACGGTGTAGATACCGGAAGCGGCGGGTTTTGCGGCTTTTTCCTCTTTCACGGCAGGTTTCGCGGCTTCCGCCTGCGGCAGTGGCTCAAACGCCGCCGGGTTATGGCGGTTTTCGAGGAATTTCAGCCCGACCTGCGGGAACAGTGCCACGGTGAGGACGTCATCAATGGCGTTCTCTGCAAGCCGGATGCCTTTTTCTGCCGCCTGGCGTTTAATGTCCGCTTCCAGTTGCGCCAGTTCCGGTTTCAGTAAATCCGCCGGGCGGCAGGTGACGGCCTCTTCGCCGTCCAGCACTCTGGCCTGTAACGCCGCGTTGACCGGCGCTGGCGTGTGGCCGTATTCGCCCTTCAGTATCCCGGCGGTTTCTTTGGCGATGGTTT\n+>NODE_216_length_189_cov_23403.1_ID_431\n+CCTGATTCAGTTTTATCTTTCGTCGGTGAGCGACGGTACTTTCTGACCTCGGTTTTTCACTCGAACGCGATTCGTGAACCGTCATCGTCGCCCTCGGAAATATCATCACCGATCACCATGTCGGCGTCAGTTTCCTGATCGAGTTTCAGCACGTCTTTGAGTGCGCCGCCGGTCGCCACAAAGCGGCGC\n+>NODE_222_length_167_cov_18131.6_ID_443\n+CACTCAAAGACGTGCTGAAACTCGATCAGGAAACTGACGCCGACATGGTGATCGGTGATGATATTTCCGAGGGCGACGATGACGGTTCACGAATCGCGTTCGAGTGAAAAACCGAGGTCAGAAAGTACCGTCGCTCCCCGACGAAAGATAAAACTGAATCAGGCTAG\n' 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