Repository 'rgrnastar'
hg clone https://toolshed.g2.bx.psu.edu/repos/iuc/rgrnastar

Changeset 23:a2b0feda6933 (2023-02-17)
Previous changeset 22:980d2a2e1180 (2022-11-01) Next changeset 24:4df95e2d7f61 (2023-02-22)
Commit message:
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar commit ae6b59a8e52fd34e2347d1fd8d34129c36779266
modified:
macros.xml
rg_rnaStar.xml
test-data/filtered3.bam
added:
test-data/Signal.Unique.str1.out.bg
test-data/Signal.Unique.str2.out.bg
test-data/Signal.UniqueMultiple.str1.out.bg
test-data/Signal.UniqueMultiple.str2.out.bg
test-data/rnastar_test_mapped_reads_PE.bam
test-data/rnastar_test_splicejunctions_PE.bed
test-data/tophat_Signal.Unique.both.read2.out.wig
test-data/tophat_rev_Signal.Unique.str1.out.bg
test-data/tophat_rev_Signal.Unique.str2.out.bg
test-data/tophat_revlib_R1.fastqsanger
test-data/tophat_revlib_R2.fastqsanger
test-data/tophat_test.fa.gz
test-data/tophat_test_reads_per_gene_PE.txt
test-data/tophat_test_reads_per_gene_PE_rev.txt
b
diff -r 980d2a2e1180 -r a2b0feda6933 macros.xml
--- a/macros.xml Tue Nov 01 16:56:55 2022 +0000
+++ b/macros.xml Fri Feb 17 20:03:27 2023 +0000
[
b'@@ -1,11 +1,12 @@\n <macros>\n-    <!-- REMEMBER to bump the version of rna_star_index_builder_data_manager\n-    whenever you make changes to the following two version tokens!\n+    <!-- REMEMBER to bump the version of @IDX_VERSION_SUFFIX@\n+    whenever you make changes to the @TOOL_VERSION@ token!\n     The data manager uses a symlink to this macro file to keep the STAR and\n-    the index versions in sync, but you should manually adjust the +galaxy\n-    version number. -->\n+    the index versions in sync, but you should manually update @IDX_VERSION_SUFFIX@ -->\n     <!-- STAR version to be used -->\n-    <token name="@VERSION@">2.7.8a</token>\n+    <token name="@TOOL_VERSION@">2.7.10b</token>\n+    <token name="@VERSION_SUFFIX@">0</token>\n+    <token name="@PROFILE@">21.01</token>\n     <!-- STAR index version compatible with this version of STAR\n     This is the STAR version that introduced the index structure expected\n     by the current version.\n@@ -14,12 +15,14 @@\n     or by looking for the versionGenome parameter in source/parametersDefault\n     of STAR\'s source code -->\n     <token name="@IDX_VERSION@">2.7.4a</token>\n+    <token name="@IDX_VERSION_SUFFIX@">1</token>\n     <token name="@IDX_DATA_TABLE@">rnastar_index2x_versioned</token>\n \n     <xml name="requirements">\n         <requirements>\n-            <requirement type="package" version="@VERSION@">star</requirement>\n-            <requirement type="package" version="1.9">samtools</requirement>\n+            <requirement type="package" version="@TOOL_VERSION@">star</requirement>\n+            <requirement type="package" version="1.16.1">samtools</requirement>\n+            <requirement type="package" version="1.12">gzip</requirement>\n             <yield />\n         </requirements>\n     </xml>\n@@ -35,7 +38,7 @@\n     </xml>\n \n     <xml name="index_selection" token_with_gene_model="0">\n-        <param argument="--genomeDir" name="genomeDir" type="select"\n+        <param argument="--genomeDir" type="select"\n         label="Select reference genome"\n         help="If your genome of interest is not listed, contact the Galaxy team">\n             <options from_data_table="@IDX_DATA_TABLE@">\n@@ -55,8 +58,8 @@\n             <citation type="doi">10.1093/bioinformatics/bts635</citation>\n         </citations>\n     </xml>\n-    <xml name="@SJDBOPTIONS@" token_optional="true">\n-         <param argument="--sjdbGTFfile" type="data" format="gff3,gtf" label="Gene model (gff3,gtf) file for splice junctions" optional="@OPTIONAL@" help="Exon junction information for mapping splices"/>\n+    <xml name="SJDBOPTIONS">\n+         <param argument="--sjdbGTFfile" type="data" format="gff3,gtf" label="Gene model (gff3,gtf) file for splice junctions" optional="false" help="Exon junction information for mapping splices"/>\n          <param argument="--sjdbOverhang" type="integer" min="1" value="100" label="Length of the genomic sequence around annotated junctions" help="Used in constructing the splice junctions database. Ideal value is ReadLength-1"/>\n     </xml>\n     <xml name="dbKeyActions">\n@@ -81,11 +84,16 @@\n     <token name="@TEMPINDEX@"><![CDATA[\n     ## Create temporary index for custom reference\n     #if str($refGenomeSource.geneSource) == \'history\':\n+        #if $refGenomeSource.genomeFastaFiles.ext == "fasta"\n+            ln -s \'$refGenomeSource.genomeFastaFiles\' refgenome.fa &&\n+        #else\n+            gunzip -c \'$refGenomeSource.genomeFastaFiles\' > refgenome.fa &&\n+        #end if\n         mkdir -p tempstargenomedir &&\n         STAR\n             --runMode genomeGenerate\n             --genomeDir \'tempstargenomedir\'\n-            --genomeFastaFiles \'${refGenomeSource.genomeFastaFiles}\'\n+            --genomeFastaFiles refgenome.fa\n             ## Handle difference between indices with/without annotations\n             #if \'GTFconditional\' in $refGenomeSource:\n                 ## GTFconditional exists only in STAR, but not STARsolo\n@@ -109,6 +117,8 @@\n                 --genomeSAindexNbases ${refGenomeSource'..b'        <expand macro="dbKeyActions" />\n+            <change_format>\n+                <when input="outWig.outWigType" value="wiggle" format="wig" />\n+            </change_format>\n+        </data>\n+        <data format="bedgraph" name="signal_unique_str2" label="${tool.name} on ${on_string}: Coverage Uniquely mapped strand 2" from_work_dir="Signal.Unique.str2.out">\n+            <filter>outWig[\'outWigType\'] != "None" and outWig[\'outWigStrand\']</filter>\n+            <expand macro="dbKeyActions" />\n+            <change_format>\n+                <when input="outWig.outWigType" value="wiggle" format="wig" />\n+            </change_format>\n+        </data>\n+        <data format="bedgraph" name="signal_uniquemultiple_str2" label="${tool.name} on ${on_string}: Coverage Uniquely + Multiple mapped strand 2" from_work_dir="Signal.UniqueMultiple.str2.out">\n+            <filter>outWig[\'outWigType\'] != "None" and outWig[\'outWigStrand\']</filter>\n+            <expand macro="dbKeyActions" />\n+            <change_format>\n+                <when input="outWig.outWigType" value="wiggle" format="wig" />\n+            </change_format>\n+        </data>\n+    </xml>\n+    <xml name="quantMode">\n+        <conditional name="quantmode_output">\n+            <param argument="--quantMode" type="select"\n+            label="Per gene/transcript output"\n+            help="STAR can provide analysis results not only with respect to the reference genome, but also with respect to genes and transcripts described by a gene model. Note: This functionality requires either the selection above of a cached index with a gene model, or a gene model provided alongside the index/reference genome in GTF or GFF3 format!">\n+                <option value="-">No per gene or transcript output</option>\n+                <option value="GeneCounts">Per gene read counts (GeneCounts)</option>\n+                <option value="TranscriptomeSAM">Transcript-based BAM output (TranscriptomeSAM)</option>\n+                <option value="TranscriptomeSAM GeneCounts">Both per gene read counts and transcript-based BAM output (TranscriptomeSAM GeneCounts)</option>\n+            </param>\n+            <when value="-" />\n+            <when value="GeneCounts" />\n+            <when value="TranscriptomeSAM">\n+                <param argument="--quantTranscriptomeBan" type="boolean" truevalue="IndelSoftclipSingleend" falsevalue="Singleend"\n+                label="Exclude alignments with indels or soft clipping from the transcriptome BAM output?"\n+                help="You will need to exclude alignments with indels and soft-clipped bases from the transcriptome BAM output for compatibility with certain transcript quantification tools, most notably RSEM. If you are using a tool, like eXpress, that can deal with indels and soft-clipped bases, you can achieve better results by leaving this option disabled." />\n+            </when>\n+            <when value="TranscriptomeSAM GeneCounts">\n+                <param argument="--quantTranscriptomeBan" type="boolean" truevalue="IndelSoftclipSingleend" falsevalue="Singleend"\n+                label="Exclude alignments with indels or soft clipping from the transcriptome BAM output?"\n+                help="You will need to exclude alignments with indels and soft-clipped bases from the transcriptome BAM output for compatibility with certain transcript quantification tools, most notably RSEM. If you are using a tool, like eXpress, that can deal with indels and soft-clipped bases, you can achieve better results by leaving this option disabled." />\n+            </when>\n+        </conditional>\n+    </xml>\n+    <xml name="quantModeNoGTF">\n+        <conditional name="quantmode_output">\n+            <param argument="--quantMode" type="select"\n+            label="Per gene/transcript output">\n+                <option value="-">No per gene or transcript output as no GTF was provided</option>\n+            </param>\n+            <when value="-" />\n+        </conditional>\n+    </xml>\n </macros>\n'
b
diff -r 980d2a2e1180 -r a2b0feda6933 rg_rnaStar.xml
--- a/rg_rnaStar.xml Tue Nov 01 16:56:55 2022 +0000
+++ b/rg_rnaStar.xml Fri Feb 17 20:03:27 2023 +0000
[
b'@@ -1,4 +1,4 @@\n-<tool id="rna_star" name="RNA STAR" version="@VERSION@+galaxy1" profile="20.01" license="MIT">\n+<tool id="rna_star" name="RNA STAR" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@" license="MIT">\n     <description>Gapped-read mapper for RNA-seq data</description>\n     <macros>\n         <import>macros.xml</import>\n@@ -65,9 +65,9 @@\n             #end if\n         #end if\n \n-        --quantMode ${quantmode_output.quantMode}\n-        #if \'TranscriptomeSAM\' in str($quantmode_output.quantMode):\n-            --quantTranscriptomeBan ${quantmode_output.quantTranscriptomeBan}\n+        --quantMode ${refGenomeSource.GTFconditional.quantmode_output.quantMode}\n+        #if \'TranscriptomeSAM\' in str($refGenomeSource.GTFconditional.quantmode_output.quantMode):\n+            --quantTranscriptomeBan ${refGenomeSource.GTFconditional.quantmode_output.quantTranscriptomeBan}\n         #end if\n \n         ## Output format parameters\n@@ -206,9 +206,7 @@\n             #end if\n \n             ## Limits\n-            --limitOutSJoneRead $algo.params.limits.limitOutSJoneRead\n-            --limitOutSJcollapsed $algo.params.limits.limitOutSJcollapsed\n-            --limitSjdbInsertNsj $algo.params.limits.limitSjdbInsertNsj\n+                @LIMITS@\n         #else:\n             ## Go with STAR\'s default algorithmic settings,\n             ## but we need to provide a reasonable default\n@@ -238,14 +236,19 @@\n                 --chimOutJunctionFormat 1\n             #end if\n         #end if\n+\n+        ##outWig:\n+        @OUTWIG@\n         &&\n         ## recompress BAM output for smaller file size\n         samtools view -b -o \'$mapped_reads\' Aligned.sortedByCoord.out.bam\n-        #if \'TranscriptomeSAM\' in str($quantmode_output.quantMode):\n+        #if \'TranscriptomeSAM\' in str($refGenomeSource.GTFconditional.quantmode_output.quantMode):\n             ## same recompression for optional transcriptome BM\n             &&\n             samtools view -b -o \'$transcriptome_mapped_reads\' Aligned.toTranscriptome.out.bam\n         #end if\n+        ##outWig:\n+        @OUTWIGOUTPUTS@\n     ]]></command>\n \n     <inputs>\n@@ -279,15 +282,22 @@\n                     <param name="GTFselect" type="select"\n                            label="Reference genome with or without an annotation"\n                            help="Select the \'... with builtin gene-model\' option to select from the list of available indexes that were built with splice junction information. Select the \'... without builtin gene-model\' option to select from the list of available indexes without annotated splice junctions, and, optionally, provide your own splice-junction annonations.">\n-                        <option value="without-gtf" selected=\'true\'>use genome reference without builtin gene-model</option>\n+                        <option value="without-gtf-with-gtf" selected=\'true\'>use genome reference without builtin gene-model but provide a gtf</option>\n+                        <option value="without-gtf">use genome reference without builtin gene-model and do not provide a gtf</option>\n                         <option value="with-gtf">use genome reference with builtin gene-model</option>\n                     </param>\n                     <when value="with-gtf">\n                         <expand macro="index_selection" with_gene_model="1" />\n+                        <expand macro="quantMode" />\n+                    </when>\n+                    <when value="without-gtf-with-gtf">\n+                        <expand macro="index_selection" with_gene_model="0" />\n+                        <expand macro="SJDBOPTIONS"/>\n+                        <expand macro="quantMode" />\n                     </when>\n                     <when value="without-gtf">\n                         <expand macro="index_selection" with_gene_model="0" />\n-                        <expand macro="@SJDBOPTIONS@" />\n+                        <expand macro="quantModeNoGTF" />\n                     </when>\n                 </conditi'..b'ompare="sim_size" delta="634" />\n+            <output name="reads_per_gene" file="tophat_test_reads_per_gene_PE.txt" />\n+            <output name="signal_unique_str1" file="tophat_Signal.Unique.both.read2.out.wig" ftype="wig"/>\n+            <output name="signal_uniquemultiple_str1" file="tophat_Signal.Unique.both.read2.out.wig" ftype="wig" />\n+        </test>\n+        <!-- test paired-end input as collection and outWig stranded -->\n+        <test expect_num_outputs="8">\n+            <conditional name="singlePaired">\n+                <param name="sPaired" value="paired_collection" />\n+                <param name="input" >\n+                    <collection type="paired">\n+                        <element name="forward" value="tophat_revlib_R1.fastqsanger" ftype="fastq"/>\n+                        <element name="reverse" value="tophat_revlib_R2.fastqsanger" ftype="fastq"/>\n+                    </collection>\n+                </param>\n+            </conditional>\n+            <conditional name="refGenomeSource">\n+                <param name="geneSource" value="history" />\n+                <param name="genomeFastaFiles" value="tophat_test.fa" />\n+                <param name="genomeSAindexNbases" value="5" />\n+                <conditional name="GTFconditional">\n+                    <param name="GTFselect" value="with-gtf" />\n+                    <param name="sjdbOverhang" value="75"/>\n+                    <param name="sjdbGTFfile" value="test1.gtf" ftype="gtf"/>\n+                    <conditional name="quantmode_output">\n+                        <param name="quantMode" value="GeneCounts"/>\n+                    </conditional>\n+                </conditional>\n+            </conditional>\n+            <section name="oformat">\n+                <param name="outSAMattributes" value="NH,HI,AS,nM,NM,MD,jM,jI,MC,ch" />\n+            </section>\n+            <section name="algo">\n+                <conditional name="params">\n+                    <param name="settingsType" value="default" />\n+                </conditional>\n+            </section>\n+            <conditional name="outWig">\n+                <param name="outWigType" value="bedGraph" />\n+            </conditional>\n+\n+            <output name="output_log" file="rnastar_test.log" compare="re_match_multiline" />\n+            <output name="splice_junctions">\n+                <assert_contents>\n+                    <has_n_lines n="2"/>\n+                    <has_line_matching expression="test_chromosome\\s+251\\s+350\\s+1\\s+1\\s+0\\s+24\\s+0\\s+33"/>\n+                    <has_line_matching expression="test_chromosome\\s+401\\s+500\\s+1\\s+1\\s+0\\s+25\\s+0\\s+36"/>\n+                </assert_contents>\n+            </output>\n+            <output name="mapped_reads" >\n+                <assert_contents>\n+                    <has_size value="4711" delta="800"/>\n+                </assert_contents>\n+            </output>\n+            <output name="reads_per_gene" file="tophat_test_reads_per_gene_PE_rev.txt" />\n+            <output name="signal_unique_str1" file="tophat_rev_Signal.Unique.str1.out.bg" ftype="bedgraph"/>\n+            <output name="signal_uniquemultiple_str1" file="tophat_rev_Signal.Unique.str1.out.bg" ftype="bedgraph"/>\n+            <output name="signal_unique_str2" file="tophat_rev_Signal.Unique.str2.out.bg" ftype="bedgraph" />\n+            <output name="signal_uniquemultiple_str2" file="tophat_rev_Signal.Unique.str2.out.bg" ftype="bedgraph" />\n+        </test>\n       </tests>\n     <help><![CDATA[\n **What it does**\n@@ -1024,7 +1128,7 @@\n In addition, the following parameters_ related to chimeric alignment are recommended for improved sensitivity\n \n - under *Output filter criteria*,\n-  **Would you like to set additional output filters?**: select `Yes\' to set \n+  **Would you like to set additional output filters?**: select `Yes` to set \n   **Maximum number of alignments to output a read\'s alignment results, plus 1** to 50 \n \n - under *Algorithmic settings*, **Configure seed, alignment and limits options**:\n'
b
diff -r 980d2a2e1180 -r a2b0feda6933 test-data/Signal.Unique.str1.out.bg
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/Signal.Unique.str1.out.bg Fri Feb 17 20:03:27 2023 +0000
b
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b
diff -r 980d2a2e1180 -r a2b0feda6933 test-data/Signal.Unique.str2.out.bg
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/Signal.Unique.str2.out.bg Fri Feb 17 20:03:27 2023 +0000
b
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b
diff -r 980d2a2e1180 -r a2b0feda6933 test-data/Signal.UniqueMultiple.str1.out.bg
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/Signal.UniqueMultiple.str1.out.bg Fri Feb 17 20:03:27 2023 +0000
b
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b
diff -r 980d2a2e1180 -r a2b0feda6933 test-data/Signal.UniqueMultiple.str2.out.bg
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/Signal.UniqueMultiple.str2.out.bg Fri Feb 17 20:03:27 2023 +0000
b
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diff -r 980d2a2e1180 -r a2b0feda6933 test-data/filtered3.bam
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Binary file test-data/filtered3.bam has changed
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diff -r 980d2a2e1180 -r a2b0feda6933 test-data/rnastar_test_mapped_reads_PE.bam
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Binary file test-data/rnastar_test_mapped_reads_PE.bam has changed
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diff -r 980d2a2e1180 -r a2b0feda6933 test-data/rnastar_test_splicejunctions_PE.bed
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/rnastar_test_splicejunctions_PE.bed Fri Feb 17 20:03:27 2023 +0000
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@@ -0,0 +1,2 @@
+test_chromosome 251 350 1 1 0 51 0 37
+test_chromosome 401 500 1 1 0 41 0 36
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diff -r 980d2a2e1180 -r a2b0feda6933 test-data/tophat_Signal.Unique.both.read2.out.wig
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/tophat_Signal.Unique.both.read2.out.wig Fri Feb 17 20:03:27 2023 +0000
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@@ -0,0 +1,290 @@
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b
diff -r 980d2a2e1180 -r a2b0feda6933 test-data/tophat_rev_Signal.Unique.str2.out.bg
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/tophat_rev_Signal.Unique.str2.out.bg Fri Feb 17 20:03:27 2023 +0000
b
@@ -0,0 +1,124 @@
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b
diff -r 980d2a2e1180 -r a2b0feda6933 test-data/tophat_revlib_R1.fastqsanger
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/tophat_revlib_R1.fastqsanger Fri Feb 17 20:03:27 2023 +0000
b
b'@@ -0,0 +1,192 @@\n+@test_mRNA_150_290_0/1\n+TCCTAAAAAGTCCGCCTCGGTCTCAGTCTCAAGTAGAAAAAGTCCCGTTGGCGATCCGTCTACGTCCGAGTAAGA\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_82_255_2/1\n+GAAAAAGTCCCGTTGCCGATCCGTCTACGTCCGAGTAATATAGTAAAGTAATAGTGGCGTATCGCAAGCTCGACG\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_72_258_4/1\n+GTAGAAAAAGTCCCGTTGCCCATCCGTCTACGTCCGAGTAAGATAATAAAGTAATAGTGGCGTATCGCAAGCTCG\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_107_286_5/1\n+AAAAAGTCCGCCTCGATCCCAGTCTCAAGTAGAAAAAGTCCCGTTGCCGATCCGTCTACGTCCGAGTAAGATAAT\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_122_299_6/1\n+CAAGTCCCGTCCTAAAAAGTCCGCCTCGATCCCAGTCTCAAGTAGAAAAAGTCCCGTTGCCGCTCCGTCTACGTC\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_58_234_7/1\n+AGTCTACGTCCGAGTCAGATAATAAACTAATAGTGGCGTATCGCAAGCTCGACGCTCAGCCGTAGGGCCGCGCGC\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_8_155_9/1\n+TACGTAGCGTCCTACTGCCCTCCTCAGTCCGATCGTCCTAAATAGTACAGTCGCTGCATCTGACGCTCGAAGTCC\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_89_230_b/1\n+TACGTCCGAGTGAGTTAATAAAGTAATAGTGGCGTATCGCAAGCTCGACGCTCAGCCGTAGGGCCGCGCGCCAGA\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_105_276_c/1\n+CCTCGATCCCAGTCTCAAGTAGAAAAAGCCCCGTTGCCGATCCGTCTACGTCCGAGTAAGATAATAAAGTAATAG\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_111_268_d/1\n+CCAGTCTCAAGTAGAAAAAGTCCCGTTGCCGATCCGTCTACGTCCGAGTAAGATAATAAAATAATAGTGGCGTAT\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_118_297_f/1\n+AGTCCCGTCCTAAAAAGTCCGCCTCGATCCCAGTCTCAAGTAGAAAAAGTCCCGTTGCCGATCCGTCTACGTCCG\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_16_194_10/1\n+TCGCAAGCTCGACGCTCAGCCGTAGGGCCGCGCGCCAAATACGTAGCGTCCTACTGCCCTCCTCAGTCCGATCGT\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_105_266_13/1\n+AGTCTCAAGTAGAAAAAGTCCCGTTGCCGATCCGTCTACGTCCGAGTAAGATAATAAAGTAATAGTGGCGGATCG\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_58_218_16/1\n+AGATAATAAAGTAATAGTGGCGTATCGCAAGCTCGACGCTCAGCCGTAGGGCCGCGCGCCAAATACGTAGCGTCC\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_46_195_17/1\n+ATCCCAAGCTCGACGCTCAGCCGTAGGGCCGCGCGACAAATATGTAGCGTCCTACTGCCCTCCTCAGTCCAATCG\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_126_282_18/1\n+AGTCCGCCTCGATCCCAGTCTCAAGTAGAAAAAGTCCCGTTGCCGATCCGTCTACGTCCGAGTAAGATAATAAAG\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_104_274_1c/1\n+TCGATCCCAGTCTCAAGTAGAAAAAGTCCCGTTGCCGATCCGTCTACGTCCGAGTAAGATAATAAAGTAATAGTG\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_44_225_1e/1\n+CCGAGTAAGATAATAAAGTAATAGTGGCGTATCGCAAGCTCGACGCTCAGCCGTAGGGCCGCGCGCCATATACGT\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_48_249_20/1\n+GTCCCGTTGCCGATCCGTCTCCGTCCGAGTAAGATAGTAAAGTAATAGTGGCGTATCGCAAGCTCGACGCTCAGC\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_110_267_22/1\n+CAGTCTCAAGTAGAAAAAGTCCCGTTGCCGATCCGTCTACGTCCGAGTAAGATAATAAAGTAATAGTGGCGTATC\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_69_229_23/1\n+ACGTCCGAGTAAGATAATAAAGTAATAGTGGCGTATCGCAAGCTAGACGCTCAGCCGTAGGGCCGCGCGCCAAAG\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_21_208_24/1\n+GTAATAGTGGCGTATCGCAAGCTCGACGCTCAGGCGTAGGGCCGCGCGCCAAATACGTAGCGTCCTACTGCCCTC\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+'..b'IIIIIIIIII\n+@test_mRNA_26_189_30/1\n+AGCTCGACGCTCAGCCGTAGGGCCGCGCGCCAAATACGTACCGTCCTACTGCCCTCCTCAGTCCGATCGTCCTAA\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_79_256_31/1\n+AGAAAAAGTCCCGTTGCCGATCCGGCTACGTCCGAGTAAGATAATAAAGTAATAGTGGCGTATGGCAAGCTCGAC\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_89_267_32/1\n+CAGTCTCAAGTAGAAAAAGTCCCGTTGCCGATCCGTCTACGTCCGAGAAAGATAATAAAGTAATAGTGCCGTATC\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_145_300_37/1\n+GCAAGTCCCGTCCTAAAAAGTCCGCCTCGATCCCAGTCTCAAGTAGAAAAAGTCCCGTTTCCGATCCGTCTACGT\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_81_228_3a/1\n+CCTACGAGTAAGATAATAAAGTAATAGTGGCGTATCGCAAGCTCGACGCTCAGCCATAGGGCCGCGCGCCAAATA\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_30_231_3c/1\n+CTACGTGCGAGTAAGATATTAAAGTAATAGTGGCGTATCGCAAGCTCGACGCTTAGCCGTAGGGCCGCGCGCCAA\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_58_220_3d/1\n+GAAGATAATAAAGTAATAGTGGCGTATCGCAACCTCGACGCTCAGCCGTAGGGCCGCGCGCCAAATACGTAGCGT\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_104_278_3e/1\n+CGCCTCGATTCCAGTCTCAAGTAGAAAAAGTCCCGTTGCCGATCCGTCTACGTCCGAGTAAGATAATAAAGTAAT\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_23_186_42/1\n+TCGACGCTCAGTCGTAGGGCCGCGCGCCAAATACGTAGCGTCCTTCTGCCCTCCTCCGTCCGATCGTCCTAAATA\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_106_253_45/1\n+AAAAGTCCCGTTGCCGATCCGTTTACGTCCGAGTAAGATAATAAAGTAATAGTGGCGTATCGCAAGCTCGACGCT\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_51_194_47/1\n+TCGCAAGCTCGACGCTCAGCCGTAGGGCCGCGCGCCAAATACGTAGCGTCCTACTGCCCTCCTCAGTCCGATCGT\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_63_229_4c/1\n+ACGTCCGAGTAAGATAATAAAGTAATAGTGGCGTATCGCAAGCTCGACACTCAGCCGTAGGGCCGCGCGCCAAAT\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_81_245_4d/1\n+CGTTGCCGATCCGTCTACGTCCGAGTAAGATTATAAAGTAATAGTGGCGTATCGCAACCTCGACGCTCAGCCGTA\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_88_257_50/1\n+TAGAAAAAGTCCCGTTGCCGATCCGTCTACGTCCGAGTAAGATAATAAAGTAATAGTGGCGTATCGCAAGCTCGA\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_9_179_52/1\n+TCAGCCGTAGGGCCGCGCGCCAAATACGTAGCGTCCTACTGCCCTCCTCAGTCCGATCGTCCTAAATGGTCCAGT\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_87_250_57/1\n+AGTCCCGTTGCCGATCCGTCTACGTCCGAGTAAGATAATAAAGTAATAGTGGCGTATCGCAAGCTCGACGCTCAT\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_53_272_5a/1\n+GATCCCAGTGTCAAGTAGAAAAAGTCCCGTTGCCGATCCGTCTACGTCCGAGTAAGATAATAAAGTAATAGTGGC\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_114_277_5b/1\n+GCCTCGATCCCAGTCTCAAGCAGAAAAAGTCCCGTTGCCGTTCCGTCTACCTCCGAGTAAGATAATAAAGTAATA\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_87_279_5f/1\n+CCGCCTCGATCCCAGTCTCAAGTAGAAAAAGTCCCGTTGCCGATCCGTCTACGTCCGAGTAAGATAATAAAGTAA\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_125_293_60/1\n+CCGTCCTAAAAAGTCCGCCTCGATCCCAGTCTCAAGTAGAAAAAGTCCCGTTGCCGATCCGTCTACGTCCGAGTA\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_111_297_61/1\n+AGTCCCGTCCTAAAAAGTCCGCCTCGATCCCAGTCTCAAGTAGAAAAAGTCCCGTTGCGGATCCGTCTACGTCCG\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_116_295_63/1\n+TCCCGTCCTAAAAAGTCCGCCTCGATCCCAGTCTCAAGTAGAAAAAGTCCCGTTGCCGATCCGTCTACGTCCGAG\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n'
b
diff -r 980d2a2e1180 -r a2b0feda6933 test-data/tophat_revlib_R2.fastqsanger
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/tophat_revlib_R2.fastqsanger Fri Feb 17 20:03:27 2023 +0000
b
b'@@ -0,0 +1,192 @@\n+@test_mRNA_150_290_0/2\n+TACGTATTTGTCGCGCGGCCCTACGGCTGAGCGTCGAGCTTGCGATCCGCCACTATTACTTTATTATCTTACTCG\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_82_255_2/2\n+GACTTAGAGCGTCAGATGCAGCGACTGGACTTTTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTAT\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_72_258_4/2\n+ACTACGGACGGACTTAGAGCGTCAGATGCAGCAACTGGACTATTTAGGACGATCGGACTGAGGAGGGCAGTAGGA\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_107_286_5/2\n+TGGACTATTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGCATTTGGCGCGCGGCCCTACGGCTGAGC\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_122_299_6/2\n+GATCGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGGCTGAGCGTCGAGCTTGCGATA\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_58_234_7/2\n+GGATGACGCCTAGGACTACGGACGGACTTAGAGCGTCAGATGCAGCGACTGGACTATTTAGGACGATCGGACTGA\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_8_155_9/2\n+TGTGACTAGACTGGAGGCGCTTGCGACTGAGCTAGGACGTGCCACTACGGGGATGACGACTAGGACTACGGACGG\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_89_230_b/2\n+AGCGTCAGGTGCAGCGACTGGACTATTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGC\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_105_276_c/2\n+ACTGGACTATTTAGGACGATCGGACTGAGGAAGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGGCTGA\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_111_268_d/2\n+CTATTTAAGACGTTCCGCCTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGGCTGAGCGTCG\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_118_297_f/2\n+GGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGGCTGAGCGTCGAGCTTGC\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_16_194_10/2\n+GACTGGATGCGCTTGCGACTGAGCTAGGACGTGCCACTACGGGGATGACGACTCGGACTACGGACGGACTTAAAG\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_105_266_13/2\n+ACTGGACTATTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGGCTGA\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_58_218_16/2\n+GGATGACGACTAGGACTACGGACGGACTTAGAACGTCAGATGCAGCGACTGGACTATTTAGGACGATCGGACTGA\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_46_195_17/2\n+GTGCCACTACGGGGATGACGACTAGGACTACGGACGGACTTAGAGCGTCAGATGCAGCGACTGGACTATTTAGGA\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_126_282_18/2\n+GGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGGCTGAGCGTCGAGCTTGCGATACGCC\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_104_274_1c/2\n+GAGTGTACTATTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTATGTGCCGCGCGGCCCTACGGCTG\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_44_225_1e/2\n+ACGTGCCACTACGGGGATGACGACTAGGACTACGGACGGACTTAGAGCGTCGGGTGCAGCGACTGGACTATTTAG\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_48_249_20/2\n+GCCACTACGGGGATGACGACTAGGACGACGGACGGACTTAGAGCGTCAGATGCAGCGACTGGACTATTTAGGACG\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_110_267_22/2\n+ACTAGTTAGGGCGATCGGACTGAGGAGGGCAGTAGGACGCTACGTAGTTGGCGCGCGGCCCTACGACTGAGCGTC\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_69_229_23/2\n+AGGACTACGGACGGACTTATAGGGTCAGATGCAGCGACTGGACTATTTAGGACGATCGGACTGAGGAGGGCAGTA\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_21_208_24/2\n+GAGGCGCTTGCGACTGAGCTAGGACGTGCCACTACGGGGATGACGACTAGGACTACGGACGGACTTAGAGCGTCA\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+'..b'IIIIIIIIII\n+@test_mRNA_26_189_30/2\n+GCTTGCGACTGAGCTAGGACGTGCCACTACGGGGATGACGACTAGGACTACGGACGGACTTAGAGCGTCAGATGC\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_79_256_31/2\n+ACGGACTTAGAGCGTCAGATGCAGCGACTGGACTATTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACG\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_89_267_32/2\n+AGCGTCAGATGCAGCGACTGGACTATTTAGGACGATCGGAGTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGG\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_145_300_37/2\n+GACGCTACGTATTTGGCGCGGGGCCCTATGGCTGAGCGTCGAGCTTGCGATACGCCACTATTACTTTAGTATATT\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_81_228_3a/2\n+GGACTGAGAGCGTCAGATGCAGCGACTGGACTATTTAGGACGATCGGACTGAGGAGGGTAGTAGGACGCTACGTA\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_30_231_3c/2\n+GCGACTGAGCTAGGACGTGCCACTACGGGGATGACGACTAGGACTACGGACGGACTTAGAGCGTCAGATGCAGCG\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_58_220_3d/2\n+GGATGACGACTAGGACTACGGACGGACTTAGAGCGTCAGATGCAGCGACTGGACTATTTAGGACGATCGGACTGA\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_104_278_3e/2\n+GACTGGACTATTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTTTTTGGCGCGCGGCCCTACGGCTG\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_23_186_42/2\n+GGCGCTTGTGACTGAGCTAGGACGTGCCACTACGGGGATGAAGACTAGGACTACGGACGGACTTAGAGCGTCAGA\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_106_253_45/2\n+CTGGACTATTTAGGTCGATCGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGGCTGAG\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_51_194_47/2\n+ACTACGGGGATGACGACTAGGACTACGGACGGACTTAGAGCGTCAGATGCAGCGACTGGACTATTTAGGACGATC\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_63_229_4c/2\n+ACGACTAGGACTACGGACGGACTTAGAGCGTCAGATGCAGGGACTGGACTATTTAGGACGATCGGACTGAGGAGG\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_81_245_4d/2\n+GGACTTAGAGCGTCAGATGCAGCGACTGGACTATTTAGGACGATCGGACTGATGAGGGCAGTAGGACGCTACGTA\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_88_257_50/2\n+GAGCGTCAGATGCAGCGACTGGACTATTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCG\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_9_179_52/2\n+CTGACTAGACTGGAGGCGCTCGCGACTGAGCTAGGACGTGCCACTACGGGGATGACGACTAGGACTACGGACGGA\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_87_250_57/2\n+AGAGCGTCAGATGCAGAGACTGGACTATTTAGGACGATCGGACTGAGGAGTGCAGTAGGACGCTACGTATTTGGC\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_53_272_5a/2\n+TACGGGGATGACGACTAGGACTACGGACGGACTTAGAGCGTCAGATGCAGCGACTGGACTATTTAGGACGATCGG\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_114_277_5b/2\n+TTTAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGCCTGAGCGTCGAGC\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_87_279_5f/2\n+AGAGCGTCAGATGCAGCGACTGGACTATTTAGGACGATCGGACCGAGGAGGGCAGTAGGACGCTACGTATTTGGC\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_125_293_60/2\n+CGGACTGAGGAGGGCAGTAGGACGCTATGTATTTGGCGCGCGGCCCTACGGCTGAGCTTCGAGGTTGCGATACGC\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_111_297_61/2\n+CTATTTAGGACGATCGGACTGGGGAGGGCAGTAGGACGCTACGGATTTGGCGCGCGGCCCTACGGCTGAGCGTCG\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n+@test_mRNA_116_295_63/2\n+TAGGACGATCGGACTGAGGAGGGCAGTAGGACGCTACGTATTTGGCGCGCGGCCCTACGGCTGAGCGTCGAGCTT\n++\n+IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII\n'
b
diff -r 980d2a2e1180 -r a2b0feda6933 test-data/tophat_test.fa.gz
b
Binary file test-data/tophat_test.fa.gz has changed
b
diff -r 980d2a2e1180 -r a2b0feda6933 test-data/tophat_test_reads_per_gene_PE.txt
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/tophat_test_reads_per_gene_PE.txt Fri Feb 17 20:03:27 2023 +0000
b
@@ -0,0 +1,5 @@
+N_unmapped 5 5 5
+N_multimapping 0 0 0
+N_noFeature 0 48 47
+N_ambiguous 0 0 0
+GENE1 95 47 48
b
diff -r 980d2a2e1180 -r a2b0feda6933 test-data/tophat_test_reads_per_gene_PE_rev.txt
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/tophat_test_reads_per_gene_PE_rev.txt Fri Feb 17 20:03:27 2023 +0000
b
@@ -0,0 +1,5 @@
+N_unmapped 0 0 0
+N_multimapping 0 0 0
+N_noFeature 0 48 0
+N_ambiguous 0 0 0
+GENE1 48 0 48