Repository 'erga_ear'
hg clone https://toolshed.g2.bx.psu.edu/repos/bgruening/erga_ear

Changeset 2:a34826ae0a73 (2024-08-30)
Previous changeset 1:b61022e1b807 (2024-07-09) Next changeset 3:3dd6be0cd8dd (2024-10-15)
Commit message:
planemo upload for repository https://github.com/ERGA-consortium/EARs/tree/main commit e293d14e82a903a4cab64dd72dfa3f3798466176
modified:
macros.xml
make_EAR.py
make_EAR.xml
test-data/EAR.pdf
test-data/EAR_2.pdf
b
diff -r b61022e1b807 -r a34826ae0a73 macros.xml
--- a/macros.xml Tue Jul 09 07:48:46 2024 +0000
+++ b/macros.xml Fri Aug 30 09:27:31 2024 +0000
b
@@ -1,6 +1,6 @@
 <macros>
-    <token name="@TOOL_VERSION@">1.0.0</token>
-    <token name="@VERSION_SUFFIX@">1</token>
+    <token name="@TOOL_VERSION@">24.08.26</token>
+    <token name="@VERSION_SUFFIX@">0</token>
     <token name="@PROFILE@">23.2</token>
     <xml name="creator">
         <creator>
@@ -21,4 +21,26 @@
             </citation>
         </citations>
     </xml>
+    <xml name="methods_tests">
+        <section name="method_data">
+                <repeat name="assembly_method_info">
+                    <param name="assembly_tools_info" value="Hifiasm: 0.19.4/HiC/l0"/>
+                </repeat>
+                <repeat name="assembly_method_info">
+                    <param name="assembly_tools_info" value="purge_dups: 1.2.6/"/>
+                </repeat>
+                <repeat name="assembly_method_info">
+                    <param name="assembly_tools_info" value="Bionano_solve: Galaxy_3.7.0"/>
+                </repeat>
+                <repeat name="assembly_method_info">
+                    <param name="assembly_tools_info" value="YaHS: 1.1"/>
+                </repeat>
+                <repeat name="curation_method_info">
+                    <param name="curation_tools_info" value="GRIT_Rapid: 2.0"/>
+                </repeat>
+                <repeat name="curation_method_info">
+                    <param name="curation_tools_info" value="HiGlass: 1.0"/>
+                </repeat>
+            </section>
+    </xml>
 </macros>
\ No newline at end of file
b
diff -r b61022e1b807 -r a34826ae0a73 make_EAR.py
--- a/make_EAR.py Tue Jul 09 07:48:46 2024 +0000
+++ b/make_EAR.py Fri Aug 30 09:27:31 2024 +0000
[
b'@@ -1,6 +1,5 @@\n \n import argparse\n-import glob\n import logging\n import math\n import os\n@@ -22,7 +21,7 @@\n # CAUTION: This is for the Galaxy version!\n # by Diego De Panis\n # ERGA Sequencing and Assembly Committee\n-EAR_version = "v24.05.20_glxy_beta"\n+EAR_version = "v24.08.26"\n \n \n def make_report(yaml_file):\n@@ -120,19 +119,9 @@\n                     fifth_column_value = target_line.split(\'\\t\')[4].strip()\n                     return fifth_column_value\n         except Exception as e:\n-            logging.warning(f"Error reading {file_path}: {str(e)}")\n+            logging.error(f"Error reading {file_path} for tool {tool} and haplotype {haplotype}: {str(e)}")\n             return \'\'\n \n-    # Getting kmer plots for curated asm\n-    def get_png_files(dir_path):\n-        png_files = glob.glob(f"{dir_path}/*.ln.png")\n-        if len(png_files) < 4:\n-            logging.warning(f"Warning: Less than 4 png files found in {dir_path}. If this is diploid, some images may be missing.")\n-            # fill missing with None\n-            while len(png_files) < 4:\n-                png_files.append(None)\n-        return png_files[:4]\n-\n     # get unique part in file names\n     def find_unique_parts(file1, file2):\n         # Split filenames into parts\n@@ -141,7 +130,6 @@\n         # Find unique parts\n         unique_parts1 = [part for part in parts1 if part not in parts2]\n         unique_parts2 = [part for part in parts2 if part not in parts1]\n-\n         return \' \'.join(unique_parts1), \' \'.join(unique_parts2)\n \n     # extract BUSCO values\n@@ -274,33 +262,34 @@\n     # Parse pipeline and generate "tree"\n     def generate_pipeline_tree(pipeline_data):\n         tree_lines = []\n-        indent = "&nbsp;" * 2  # Adjust indent spacing as needed\n-\n-        for tool_version_param in pipeline_data:\n-            parts = tool_version_param.split(\'|\')\n-            tool_version = parts[0]\n-            tool, version = tool_version.split(\'_v\') if \'_v\' in tool_version else (tool_version, "NA")\n+        indent = "&nbsp;" * 2  # Adjust indent spacing\n \n-            # Handle parameters: join all but the first (which is tool_version) with \', \'\n-            param_text = \', \'.join(parts[1:]) if len(parts) > 1 else "NA"\n+        if isinstance(pipeline_data, dict):\n+            for tool, version_param in pipeline_data.items():\n+                # Tool line\n+                tool_line = f"- <b>{tool}</b>"\n+                tree_lines.append(tool_line)\n \n-            # Tool line\n-            tool_line = f"- <b>{tool}</b>"\n-            tree_lines.append(tool_line)\n+                # Convert version_param to string and split\n+                version_param_str = str(version_param)\n+                parts = version_param_str.split(\'/\')\n+                version = parts[0]\n+                params = [p for p in parts[1:] if p]  # This will remove empty strings\n \n-            # Version line\n-            version_line = f"{indent*2}|_ <i>ver:</i> {version}"\n-            tree_lines.append(version_line)\n+                # Version line\n+                version_line = f"{indent * 2}|_ <i>ver:</i> {version}"\n+                tree_lines.append(version_line)\n \n-            # Param line(s)\n-            if param_text != "NA":\n-                for param in param_text.split(\',\'):\n-                    param = param.strip()\n-                    param_line = f"{indent*2}|_ <i>key param:</i> {param if param else \'NA\'}"\n+                # Param line(s)\n+                if params:\n+                    for param in params:\n+                        param_line = f"{indent * 2}|_ <i>key param:</i> {param}"\n+                        tree_lines.append(param_line)\n+                else:\n+                    param_line = f"{indent * 2}|_ <i>key param:</i> NA"\n                     tree_lines.append(param_line)\n-            else:\n-                param_line = f"{indent*2}|_ <i>key param:</i> NA"\n-                tree_lines.append(param_line)\n+        else:\n+            tree_lines.append("Invalid pipeline data form'..b"l.', 'BUSCO frag.', 'BUSCO miss.']}\n     for asm_stage, stage_properties in asm_data.items():\n-        asm_stage_elements = [element for element in stage_properties.keys() if element != 'pipeline']\n+        asm_stage_elements = list(stage_properties.keys())\n         for i, haplotypes in enumerate(asm_stage_elements):\n             haplotype_properties = stage_properties[haplotypes]\n             if isinstance(haplotype_properties, dict):\n@@ -580,7 +574,7 @@\n     styles.add(ParagraphStyle(name='subTitleStyle', fontName='Courier', fontSize=16))\n     styles.add(ParagraphStyle(name='normalStyle', fontName='Courier', fontSize=12))\n     styles.add(ParagraphStyle(name='midiStyle', fontName='Courier', fontSize=10))\n-    styles.add(ParagraphStyle(name='LinkStyle', fontName='Courier', fontSize=10, textColor='blue', underline=True))\n+    # styles.add(ParagraphStyle(name='LinkStyle', fontName='Courier', fontSize=10, textColor='blue', underline=True))\n     styles.add(ParagraphStyle(name='treeStyle', fontName='Courier', fontSize=10, leftIndent=12))\n     styles.add(ParagraphStyle(name='miniStyle', fontName='Courier', fontSize=8))\n     styles.add(ParagraphStyle(name='FileNameStyle', fontName='Courier', fontSize=6))\n@@ -659,7 +653,7 @@\n \n     # Iterate over haplotypes in the Curated category to get data for EBP metrics\n     curated_assemblies = yaml_data.get('ASSEMBLIES', {}).get('Curated', {})\n-    haplotype_names = [key for key in curated_assemblies.keys() if key != 'pipeline']\n+    haplotype_names = list(curated_assemblies.keys())\n \n     for haplotype in haplotype_names:\n         properties = curated_assemblies[haplotype]\n@@ -756,7 +750,7 @@\n     # Store BUSCO version and lineage information from each file in list\n     busco_info_list = []\n     for asm_stages, stage_properties in asm_data.items():\n-        for haplotype_keys, haplotype_properties in stage_properties.items():\n+        for i, haplotype_properties in stage_properties.items():\n             if isinstance(haplotype_properties, dict):\n                 if 'busco_short_summary_txt' in haplotype_properties:\n                     busco_version, lineage_info = extract_busco_info(haplotype_properties['busco_short_summary_txt'])\n@@ -787,9 +781,9 @@\n     tool_count = 0\n \n     # Add title and images for each step\n-    for idx, (asm_stages, stage_properties) in enumerate(asm_data.items(), 1):\n+    for asm_stages, stage_properties in asm_data.items():\n         if asm_stages == 'Curated':\n-            tool_elements = [element for element in stage_properties.keys() if element != 'pipeline']\n+            tool_elements = list(stage_properties.keys())\n \n             images_with_names = []\n \n@@ -825,7 +819,7 @@\n \n             # Add images and names to the elements in pairs\n             for i in range(0, len(images_with_names), 4):  # Process two images (and their names) at a time\n-                elements_to_add = images_with_names[i:i + 4]\n+                elements_to_add = images_with_names[i: i + 4]\n \n                 # Create table for the images and names\n                 table = Table(elements_to_add)\n@@ -856,7 +850,6 @@\n \n     # Iterate over haplotypes in the Curated category to get K-mer spectra images\n     curated_assemblies = yaml_data.get('ASSEMBLIES', {}).get('Curated', {})\n-    haplotype_names = [key for key in curated_assemblies.keys() if key != 'pipeline']\n \n     # Get paths for spectra files\n     spectra_files = {\n@@ -974,9 +967,9 @@\n     tool_count = 0\n \n     # Add title and images for each step\n-    for idx, (asm_stages, stage_properties) in enumerate(asm_data.items(), 1):\n+    for asm_stages, stage_properties in asm_data.items():\n         if asm_stages == 'Curated':  # Check if the current stage is 'Curated'\n-            tool_elements = [element for element in stage_properties.keys() if element != 'pipeline']\n+            tool_elements = list(stage_properties.keys())\n \n             for haplotype in tool_elements:\n                 haplotype_properties = stage_properties[haplotype]\n"
b
diff -r b61022e1b807 -r a34826ae0a73 make_EAR.xml
--- a/make_EAR.xml Tue Jul 09 07:48:46 2024 +0000
+++ b/make_EAR.xml Fri Aug 30 09:27:31 2024 +0000
[
@@ -39,7 +39,6 @@
 # ASSEMBLY DATA
 ASSEMBLIES:
     Pre-curation:
-        pipeline: [Hifiasm_v0.19.4|HiC|l0, Purge_Dups_v1.2.6|, Bionano_vGalaxy_3.7.0, YaHS_v1.1]
         '${pre_curation_assembly_data.haplotype_selection}': 
             gfastats--nstar-report_txt: '${pre_curation_assembly_data.gfstats_nstar_report_precuration}'
             busco_short_summary_txt: '${pre_curation_assembly_data.busco_short_summary_precuration}'
@@ -52,7 +51,6 @@
             merqury_completeness_stats: '${pre_curation_assembly_data.hap2_precuration_data.merqury_completeness_stats_hap2_precuration}'
 
     Curated:
-        pipeline: [GRIT_rapid_v2.0, HiGlass_v1.0]
         '${pre_curation_assembly_data.haplotype_selection}':
             gfastats--nstar-report_txt: '${curated_assembly_data.gfstats_nstar_report_curated}'
             busco_short_summary_txt: '${curated_assembly_data.busco_short_summary_curated}'
@@ -80,7 +78,6 @@
 # ASSEMBLY DATA
 ASSEMBLIES:
     Pre-curation:
-        pipeline: [Hifiasm_v0.19.4|HiC|l0, Purge_Dups_v1.2.6|, Bionano_vGalaxy_3.7.0, YaHS_v1.1]
         '${pre_curation_assembly_data.haplotype_selection}': 
             gfastats--nstar-report_txt: '${pre_curation_assembly_data.gfstats_nstar_report_precuration}'
             busco_short_summary_txt: '${pre_curation_assembly_data.busco_short_summary_precuration}'
@@ -88,7 +85,6 @@
             merqury_completeness_stats: '${pre_curation_assembly_data.merqury_completeness_stats_precuration}'
 
     Curated:
-        pipeline: [GRIT_rapid_v2.0, HiGlass_v1.0]
         '${pre_curation_assembly_data.haplotype_selection}':
             gfastats--nstar-report_txt: '${curated_assembly_data.gfstats_nstar_report_curated}'
             busco_short_summary_txt: '${curated_assembly_data.busco_short_summary_curated}'
@@ -102,6 +98,18 @@
             blobplot_cont_png: '${curated_assembly_data.blobplot_cont_curated}'
 #end if
 
+# METHODS DATA
+PIPELINES:
+  Assembly:
+    #for $repeat in $method_data.assembly_method_info:
+    ${repeat.assembly_tools_info}
+    #end for
+
+  Curation:
+    #for $repeat in $method_data.curation_method_info:
+    ${repeat.curation_tools_info}
+    #end for
+
 # CURATION NOTES
 NOTES:
     Obs_Haploid_num: '${curation_notes.obs_haploid_num}'
@@ -131,7 +139,7 @@
             <param name="tags" type="select" label="Select a valid tag"> 
                 <option value="ERGA-BGE" selected="true">ERGA-BGE</option>
                 <option value="ERGA-Pilot">ERGA-Pilot</option>
-                <option value="ERGA-Satellite">ERGA-Satellite</option>
+                <option value="ERGA-Community">ERGA-Community</option>
             </param>
         </section>
 
@@ -150,7 +158,6 @@
 
         <!-- Input parameters for Assembly data precurated -->
         <section name="pre_curation_assembly_data" title="Pre-Curation Assembly Information">
-            <param name="pipeline_tools_precuration" type="text" label="Input tool names along with version and paramters used" help="[Insert ToolA_v1.2.3|ParamX|ParamY, Insert ToolB_v2.3.4] valid input is empty or between brackets ToolName followed by _v followed by versionNumber followed by | followed by keyToolParameter"/>
             <param name="haplotype_selection" type="select"  label="Select a valid haplotype"> 
                 <option value="hap1">hap1</option>
                 <option value="pri">pri</option>
@@ -177,7 +184,6 @@
         
         <!-- Input parameters for Assembly data Curated -->    
         <section name="curated_assembly_data" title="Curated Assembly Data Information">    
-            <param name="pipeline_tools_curation" type="text" label="Input tool names along with version and paramters used" help="[Insert ToolA_v1.2.3|ParamX|ParamY, Insert ToolB_v2.3.4] valid input is empty or between brackets ToolName followed by _v followed by versionNumber followed by | followed by keyToolParameter"/>
             <param name="gfstats_nstar_report_curated" type="data" format="txt" label="Select curated gfastats--nstar report file"/>
             <param name="busco_short_summary_curated" type="data" format="txt" label="Select curated busco_short_summary.txt file"/>
             <param name="mercury_qv_curated" type="data" format="txt" label="Select curated Merqury results .qv file"/>
@@ -210,6 +216,16 @@
             </conditional>
         </section>
         
+        <!-- Input parameters for Methods data -->
+        <section name="method_data" title="Method Information">
+            <repeat name='assembly_method_info' title="Specify the method used for Assembly" min="1" default="2">
+                <param name="assembly_tools_info" type="text" label="Input tool names along with version and paramters used for assembly" optional="False" help="Specify ToolName:Tool_Version/Tool_parameters"/>
+            </repeat>
+            <repeat name='curation_method_info' title="Specify the method used for Curation" min="1" default="2">
+                <param name="curation_tools_info" type="text" label="Input tool names along with version and paramters used for curation" optional="False" help="Specify ToolName:Tool_Version/Tool_parameters"/>
+            </repeat>
+        </section>
+
         <!-- Input parameters for Curation notes --> 
         <section name="curation_notes" title="Curation Notes">
             <param name="obs_haploid_num" type="text" optional="False" label="Insert observed haploid number"/>
@@ -282,6 +298,7 @@
                     <param name="hap2_exists_curated" value="no"/>
                 </conditional>
             </section>
+            <expand macro="methods_tests"></expand>
             <section name="curation_notes">
                 <param name="obs_haploid_num" value="28"/>
                 <param name="obs_sex" value="XX"/>
@@ -356,6 +373,7 @@
                     <param name="blobplot_cont_hap2_curated" value="blob2.png"/>
                 </conditional>
             </section>
+            <expand macro="methods_tests"></expand>
             <section name="curation_notes">
                 <param name="obs_haploid_num" value="28"/>
                 <param name="obs_sex" value="XX"/>
b
diff -r b61022e1b807 -r a34826ae0a73 test-data/EAR.pdf
--- a/test-data/EAR.pdf Tue Jul 09 07:48:46 2024 +0000
+++ b/test-data/EAR.pdf Fri Aug 30 09:27:31 2024 +0000
[
@@ -153,7 +153,7 @@
 endobj
 20 0 obj
 <<
-/Author (\(anonymous\)) /CreationDate (D:20240704122313+00'00') /Creator (\(unspecified\)) /Keywords () /ModDate (D:20240704122313+00'00') /Producer (ReportLab PDF Library - www.reportlab.com) 
+/Author (\(anonymous\)) /CreationDate (D:20240829084627+00'00') /Creator (\(unspecified\)) /Keywords () /ModDate (D:20240829084627+00'00') /Producer (ReportLab PDF Library - www.reportlab.com) 
   /Subject (\(unspecified\)) /Title (\(anonymous\)) /Trapped /False
 >>
 endobj
@@ -164,10 +164,10 @@
 endobj
 22 0 obj
 <<
-/Filter [ /ASCII85Decode /FlateDecode ] /Length 1481
+/Filter [ /ASCII85Decode /FlateDecode ] /Length 1471
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diff -r b61022e1b807 -r a34826ae0a73 test-data/EAR_2.pdf
--- a/test-data/EAR_2.pdf Tue Jul 09 07:48:46 2024 +0000
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