Repository 'scanpy_cluster_reduce_dimension'
hg clone https://toolshed.g2.bx.psu.edu/repos/iuc/scanpy_cluster_reduce_dimension

Changeset 3:ac8e59e87ce3 (2019-12-12)
Previous changeset 2:dc2de14881ba (2019-12-05) Next changeset 4:766be978777a (2019-12-18)
Commit message:
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 3b41d687ff30583540d055f6995de00530cca81d"
modified:
cluster_reduce_dimension.xml
macros.xml
test-data/pp.highly_variable_genes.krumsiek11-cell_ranger.h5ad
added:
test-data/pl.rank_genes_groups_dotplot.rank_genes_groups.krumsiek11.png
test-data/pl.rank_genes_groups_heatmap.rank_genes_groups.krumsiek11.png
test-data/pl.rank_genes_groups_matrixplot.rank_genes_groups.krumsiek11.png
test-data/pl.rank_genes_groups_stacked_violin.rank_genes_groups.krumsiek11.png
b
diff -r dc2de14881ba -r ac8e59e87ce3 cluster_reduce_dimension.xml
--- a/cluster_reduce_dimension.xml Thu Dec 05 07:11:19 2019 -0500
+++ b/cluster_reduce_dimension.xml Thu Dec 12 09:25:42 2019 -0500
[
b'@@ -7,7 +7,7 @@\n             <param argument="dtype" type="text" value="float32" label="Numpy data type string to which to convert the result" help=""/>\n             <conditional name="pca">\n                 <param argument="chunked" type="select" label="Type of PCA?">\n-                    <option value="True">Incremental PCA on segments (incremental PCA automatically zero centers and ignores settings of `random_seed` and `svd_solver`)</option>\n+                    <option value="True">Incremental PCA on segments (incremental PCA automatically zero centers and ignores settings of \'random_seed\' and \'svd_solver\')</option>\n                     <option value="False" selected="true">Full PCA</option>\n                 </param>\n                 <when value="True">\n@@ -74,7 +74,7 @@\n sc.tl.louvain(\n     adata=adata,\n     flavor = \'$method.flavor.flavor\',\n-    #if $method.flavor.flavor == \'vtraag\' and $method.flavor.resolution\n+    #if $method.flavor.flavor == \'vtraag\'\n     resolution=$method.flavor.resolution,\n     #end if\n     random_state=$method.random_state,\n@@ -185,16 +185,16 @@\n         <expand macro="inputs_anndata"/>\n         <conditional name="method">\n             <param argument="method" type="select" label="Method used for plotting">\n-                <option value="tl.louvain">Cluster cells into subgroups, using `tl.louvain`</option>\n-                <option value="tl.leiden">Cluster cells into subgroups, using `tl.leiden`</option>\n-                <option value="pp.pca">Computes PCA (principal component analysis) coordinates, loadings and variance decomposition, using `pp.pca`</option>\n-                <option value="tl.pca">Computes PCA (principal component analysis) coordinates, loadings and variance decomposition, using `tl.pca`</option>\n-                <option value="tl.diffmap">Diffusion Maps, using `tl.diffmap`</option>\n-                <option value="tl.tsne">t-distributed stochastic neighborhood embedding (tSNE), using `tl.tsne`</option>\n-                <option value="tl.umap">Embed the neighborhood graph using UMAP, using `tl.umap`</option>\n-                <option value="tl.draw_graph">Force-directed graph drawing, using `tl.draw_graph`</option>\n-                <option value="tl.dpt">Infer progression of cells through geodesic distance along the graph, using `tl.dpt`</option>\n-                <option value="tl.paga">Generate cellular maps of differentiation manifolds with complex topologies, using `tl.paga`</option>\n+                <option value="tl.louvain">Cluster cells into subgroups, using \'tl.louvain\'</option>\n+                <option value="tl.leiden">Cluster cells into subgroups, using \'tl.leiden\'</option>\n+                <option value="pp.pca">Computes PCA (principal component analysis) coordinates, loadings and variance decomposition, using \'pp.pca\'</option>\n+                <option value="tl.pca">Computes PCA (principal component analysis) coordinates, loadings and variance decomposition, using \'tl.pca\'</option>\n+                <option value="tl.diffmap">Diffusion Maps, using \'tl.diffmap\'</option>\n+                <option value="tl.tsne">t-distributed stochastic neighborhood embedding (tSNE), using \'tl.tsne\'</option>\n+                <option value="tl.umap">Embed the neighborhood graph using UMAP, using \'tl.umap\'</option>\n+                <option value="tl.draw_graph">Force-directed graph drawing, using \'tl.draw_graph\'</option>\n+                <option value="tl.dpt">Infer progression of cells through geodesic distance along the graph, using \'tl.dpt\'</option>\n+                <option value="tl.paga">Generate cellular maps of differentiation manifolds with complex topologies, using \'tl.paga\'</option>\n             </param>\n             <when value="tl.louvain">\n                 <conditional name="flavor">\n@@ -203,7 +203,7 @@\n                         <option value="igraph">igraph</option>\n                     </param>\n                     <when value="vtraag">\n-                        <param argume'..b'nt="init_pos" type="select" label="How to initialize the low dimensional embedding" help="Called \'init\' in the original UMAP">\n                     <option value="paga">Position from paga</option>\n                     <option value="spectral" selected="true">Spectral embedding of the graph</option>\n                     <option value="random">Initial embedding positions at random</option>\n@@ -259,17 +259,17 @@\n                 <expand macro="param_random_state"/>\n                 <param argument="init_pos" type="text" optional="true" value="" label="Precomputed coordinates for initialization" help="It should be a valid 2d observation (e.g. paga)"/>\n                 <param argument="adjacency" type="data" format="mtx" optional="true" label="Sparse adjacency matrix of the graph" help="If not set, it uses the unstructured annotation (uns) / neighbors / connectivities"/>\n-                <param argument="key_ext" type="text" optional="true" value="" label="External key" help="If not set, it appends `layout`"/>\n+                <param argument="key_ext" type="text" optional="true" value="" label="External key" help="If not set, it appends \'layout\'"/>\n             </when>\n             <when value="tl.dpt">\n                 <param argument="n_dcs" type="integer" min="0" value="10" label="Number of diffusion components to use" help=""/>\n                 <param argument="n_branchings" type="integer" min="0" value="0" label="Number of branchings to detect" help=""/>\n-                <param argument="min_group_size" type="float" min="0" value="0.01" label="Min group size" help="During recursive splitting of branches (\'dpt groups\') for `n_branchings` &gt; 1, do not consider groups that contain less than `min_group_size` data points. If a float, `min_group_size` refers to a fraction of the total number of data points."/>\n+                <param argument="min_group_size" type="float" min="0" value="0.01" label="Min group size" help="During recursive splitting of branches (\'dpt groups\') for \'n_branchings\' &gt; 1, do not consider groups that contain less than \'min_group_size\' data points. If a float, \'min_group_size\' refers to a fraction of the total number of data points."/>\n                 <param argument="allow_kendall_tau_shift" type="boolean" truevalue="True" falsevalue="False" checked="true" label="Allow Kendal tau shift?" help="If a very small branch is detected upon splitting, shift away from maximum correlation in Kendall tau criterion of Haghverdi et al (2016) to stabilize the splitting."/>\n             </when>\n             <when value="tl.paga">\n-                <param argument="groups" type="text" value="louvain" label="Key for categorical in the input" help="You can pass your predefined groups by choosing any categorical annotation of observations (`adata.obs`)."/>\n-                <param argument="use_rna_velocity" type="boolean" truevalue="False" falsevalue="False" checked="false" label="Use RNA velocity to orient edges in the abstracted graph and estimate transitions?" help="Requires that `adata.uns` contains a directed single-cell graph with key `[\'velocyto_transitions\']`. This feature might be subject to change in the future."/>\n+                <param argument="groups" type="text" value="louvain" label="Key for categorical in the input" help="You can pass your predefined groups by choosing any categorical annotation of observations (\'adata.obs\')."/>\n+                <param argument="use_rna_velocity" type="boolean" truevalue="False" falsevalue="False" checked="false" label="Use RNA velocity to orient edges in the abstracted graph and estimate transitions?" help="Requires that \'adata.uns\' contains a directed single-cell graph with key \'[\'velocyto_transitions\']\'. This feature might be subject to change in the future."/>\n                 <param argument="model" type="select" label="PAGA connectivity model" help="">\n                     <option value="v1.2">v1.2</option>\n                     <option value="v1.0">v1.0</option>\n'
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diff -r dc2de14881ba -r ac8e59e87ce3 macros.xml
--- a/macros.xml Thu Dec 05 07:11:19 2019 -0500
+++ b/macros.xml Thu Dec 12 09:25:42 2019 -0500
[
b'@@ -1,7 +1,7 @@\n <macros>\n     <token name="@version@">1.4.4.post1</token>\n     <token name="@profile@">19.01</token>\n-    <token name="@galaxy_version@"><![CDATA[@version@+galaxy0]]></token>\n+    <token name="@galaxy_version@"><![CDATA[@version@+galaxy1]]></token>\n     <xml name="requirements">\n         <requirements>\n             <requirement type="package" version="@version@">scanpy</requirement>\n@@ -351,10 +351,10 @@\n         <option value="gist_ncar">gist_ncar (Miscellaneous)</option>\n     </xml>\n     <xml name="param_groupby">\n-        <param argument="groupby" type="text" value="" optional="true" label="The key of the observation grouping to consider" help="If it is given, the heatmap is ordered by the respective group. It is expected that to be a categorical. If it is not a categorical observation, it would be subdivided into `num_categories`."/>\n+        <param argument="groupby" type="text" value="" optional="true" label="The key of the observation grouping to consider" help="If it is given, the plot is ordered by the respective group. It is expected that to be a categorical. If it is not a categorical observation, it would be subdivided into \'num_categories\'."/>\n     </xml>\n     <xml name="param_use_raw">\n-        <param argument="use_raw" type="boolean" truevalue="True" falsevalue="False" checked="false" label="Use `raw` attribute of input if present" help=""/>\n+        <param argument="use_raw" type="boolean" truevalue="True" falsevalue="False" checked="false" label="Use \'raw\' attribute of input if present" help=""/>\n     </xml>\n     <xml name="param_log">\n         <param argument="log" type="boolean" truevalue="True" falsevalue="False" checked="false" label="Use the log of the values?"/>\n@@ -378,12 +378,12 @@\n     <xml name="pl_var_names">\n         <conditional name="var_names">\n             <param name="type" type="select" label="Variables to plot (columns of the heatmaps)" >\n-                <option value="all">All variables in `adata.var_names`</option>\n-                <option value="custom">Subset of variables in `adata.var_names`</option>\n+                <option value="all">All variables in \'adata.var_names\'</option>\n+                <option value="custom">Subset of variables in \'adata.var_names\'</option>\n             </param>\n             <when value="all"/>\n             <when value="custom">\n-                <param argument="var_names" type="text" value="" label="List of variables to plot" help="They should be a valid subset of `adata.var_names`, and separated by comma"/>\n+                <param argument="var_names" type="text" value="" label="List of variables to plot" help="They should be a valid subset of \'adata.var_names\', and separated by comma"/>\n             </when>\n         </conditional>\n     </xml>\n@@ -404,27 +404,20 @@\n         <param argument="var_group_rotation" type="float" value="" optional="true" label="Label rotation degrees" help="By default, labels larger than 4 characters are rotated 90 degrees"/>\n     </xml>\n     <xml name="param_layer">\n-        <param argument="layer" type="text" value="" label="Name of the AnnData object layer that wants to be plotted" help="By default `adata.raw.X` is plotted. If `use_raw=False` is set, then `adata.X` is plotted. If layer is set to a valid layer name, then the layer is plotted. layer takes precedence over `use_raw`."/>\n+        <param argument="layer" type="text" value="" label="Name of the AnnData object layer that wants to be plotted" help="By default \'adata.raw.X\' is plotted. If \'use_raw=False\' is set, then \'adata.X\' is plotted. If layer is set to a valid layer name, then the layer is plotted. layer takes precedence over \'use_raw\'."/>\n     </xml>\n     <token name="@CMD_param_plot_inputs@"><![CDATA[\n     adata,\n     save=\'.$format\',\n     show=False,\n     ]]></token>\n-    <xml name="params_plots">\n+    <xml name="params_inputs">\n         <expand macro="pl_var_names"/>\n         <expand macro="param_groupby"/>\n-        <expand macro="param_log"/>'..b'_pl_heatmap@"><![CDATA[\n+    swap_axes=$method.swap_axes,\n+    show_gene_labels=$method.show_gene_labels,\n+    cmap=\'$method.matplotlib_pyplot_imshow.cmap\',\n+    #if str($method.matplotlib_pyplot_imshow.interpolation) != \'None\'\n+    interpolation=\'$method.matplotlib_pyplot_imshow.interpolation\',\n+    #end if\n+    #if $method.matplotlib_pyplot_imshow.alpha\n+    alpha=$method.matplotlib_pyplot_imshow.alpha,\n+    #end if\n+    #if $method.matplotlib_pyplot_imshow.vmin\n+    vmin=$method.matplotlib_pyplot_imshow.vmin,\n+    #end if\n+    #if $method.matplotlib_pyplot_imshow.vmax\n+    vmax=$method.matplotlib_pyplot_imshow.vmax,\n+    #end if\n+    origin=\'$method.matplotlib_pyplot_imshow.origin\'\n+    ]]>\n+    </token>\n+    <xml name="pl_rank_genes_groups_ext">\n+        <expand macro="param_groups"/>\n+        <expand macro="param_n_genes"/>\n+        <expand macro="param_key"/>\n+    </xml>\n+    <token name="@CMD_pl_rank_genes_groups_ext@"><![CDATA[\n+    @CMD_params_groups@\n+    #if str($method.n_genes) != \'\'\n+    n_genes=$method.n_genes,\n+    #end if\n+    #if str($method.key) != \'\'\n+    key=\'$method.key\',\n+    #end if\n+    ]]>\n+    </token>\n+    <xml name="pl_matrixplot">\n+        <expand macro="param_swap_axes"/>\n+        <section name="matplotlib_pyplot_pcolor" title="Parameters for matplotlib.pyplot.pcolor">\n+            <param argument="cmap" type="select" label="Color palette">\n+                <expand macro="seaborn_color_palette_options"/>\n+            </param>\n+            <param argument="vmin" type="float" value="" optional="true" label="Minimum value to anchor the colormap" help=""/>\n+            <param argument="vmax" type="float" value="" optional="true" label="Maximum value to anchor the colormap" help=""/>\n+            <expand macro="param_matplotlib_pyplot_edgecolors"/>\n+            <expand macro="param_alpha"/>\n+            <param argument="snap" type="boolean" truevalue="True" falsevalue="False" checked="false" label="Snap the mesh to pixel boundaries?" help=""/>\n+        </section>\n+    </xml>\n+    <token name="@CMD_pl_matrixplot@"><![CDATA[\n+    swap_axes=$method.swap_axes,\n+    cmap=\'$method.matplotlib_pyplot_pcolor.cmap\',\n+    #if $method.matplotlib_pyplot_pcolor.vmin\n+    vmin=$method.matplotlib_pyplot_pcolor.vmin,\n+    #end if\n+    #if $method.matplotlib_pyplot_pcolor.vmax\n+    vmax=$method.matplotlib_pyplot_pcolor.vmax,\n+    #end if\n+    edgecolors=\'$method.matplotlib_pyplot_pcolor.edgecolors\',\n+    #if $method.matplotlib_pyplot_pcolor.alpha\n+    alpha=$method.matplotlib_pyplot_pcolor.alpha,\n+    #end if\n+    snap=$method.matplotlib_pyplot_pcolor.snap\n+    ]]>\n+    </token>\n+    <xml name="pl_stacked_violin">\n+        <expand macro="param_swap_axes"/>\n+        <section name="violin_plot" title="Violin plot attributes">\n+            <expand macro="conditional_stripplot"/>\n+            <expand macro="param_scale"/>\n+        </section>\n+        <param argument="row_palette" type="select" label="Colors to use in each of the stacked violin plots">\n+            <option value="muted">muted</option>\n+            <expand macro="seaborn_color_palette_options"/>\n+        </param>\n+        <param argument="standard_scale" type="select" label="Standardize a dimension between 0 and 1" help="Each variable or observation is subtracted by the minimum and divided each by its maximum.">\n+            <option value="None">No standardization</option>\n+            <option value="var">Standardization on variable</option>\n+            <option value="obs">Standardization on observation</option>\n+        </param>\n+        <expand macro="seaborn_violinplot"/>\n+    </xml>\n+    <token name="@CMD_pl_stacked_violin@"><![CDATA[\n+    swap_axes=$method.swap_axes,\n+    @CMD_conditional_stripplot@\n+    scale=\'$method.violin_plot.scale\',\n+    row_palette=\'$method.row_palette\',\n+    #if str($method.standard_scale) != \'None\'\n+    standard_scale=\'$method.standard_scale\',\n+    #end if\n+    @CMD_params_seaborn_violinplot@\n+    ]]>\n+    </token>\n </macros>\n'
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