Repository 'data_manager_primer_scheme_bedfiles'
hg clone https://toolshed.g2.bx.psu.edu/repos/iuc/data_manager_primer_scheme_bedfiles

Changeset 0:ae730ecdc7e7 (2020-04-19)
Next changeset 1:cce5d9327cd6 (2020-04-24)
Commit message:
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/data_managers/data_manager_primer_scheme_bedfiles commit f78536f45aa5ce801251a9725e468a47de1be44a"
added:
data_manager/install_primer_scheme_bedfiles.py
data_manager/install_primer_scheme_bedfiles.xml
data_manager/test-data/sample1.bed
data_manager_conf.xml
tool-data/primer_scheme_bedfiles.loc.sample
tool_data_table_conf.xml.sample
b
diff -r 000000000000 -r ae730ecdc7e7 data_manager/install_primer_scheme_bedfiles.py
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/data_manager/install_primer_scheme_bedfiles.py Sun Apr 19 09:22:19 2020 -0400
[
@@ -0,0 +1,141 @@
+#!/usr/bin/env python
+
+from __future__ import print_function, division
+
+import argparse
+import json
+import os
+import os.path
+import re
+import sys
+import shutil
+
+import requests
+
+DATA_TABLE_NAME = "primer_scheme_bedfiles"
+
+
+def fetch_artic_primers(output_directory, primers):
+    primer_sets = {
+        "ARTICv1": "https://raw.githubusercontent.com/artic-network/artic-ncov2019/master/primer_schemes/nCoV-2019/V1/nCoV-2019.bed",
+        "ARTICv2": "https://raw.githubusercontent.com/artic-network/artic-ncov2019/master/primer_schemes/nCoV-2019/V2/nCoV-2019.bed",
+        "ARTICv3": "https://raw.githubusercontent.com/artic-network/artic-ncov2019/master/primer_schemes/nCoV-2019/V3/nCoV-2019.bed",
+    }
+
+    data = []
+    for name, url in primer_sets.items():
+        if name not in primers:
+            continue
+        response = requests.get(url)
+        if response.status_code != 200:
+            print(
+                "Error: download of",
+                url,
+                "failed with code",
+                response.status_code,
+                file=sys.stderr,
+            )
+            exit(response.status_code)
+        bed_output_filename = os.path.join(output_directory, name + ".bed")
+        open(bed_output_filename, "w").write(response.text)
+        description = name[:-2] + " " + name[-2:] + " primer set"
+        data.append(dict(value=name, path=bed_output_filename, description=description))
+    return data
+
+
+def install_primer_file(
+    output_directory, input_filename, primer_name, primer_description
+):
+    name = re.sub(r"\W", "", str(primer_name).replace(" ", "_"))
+    output_filename = os.path.join(output_directory, name + ".bed")
+    shutil.copyfile(input_filename, output_filename)
+    data = [dict(value=name, description=primer_description, path=output_filename)]
+    return data
+
+
+class SplitArgs(argparse.Action):
+    def __call__(self, parser, namespace, values, option_string=None):
+        setattr(namespace, self.dest, values.split(","))
+
+
+if __name__ == "__main__":
+    parser = argparse.ArgumentParser(
+        description="Fetch ARTIC SARS-CoV-2 primer files for Galaxy/IRIDA use"
+    )
+    parser.add_argument(
+        "--output_directory", default="tmp", help="Directory to write output to"
+    )
+    primer_file = parser.add_argument_group()
+    primer_file.add_argument(
+        "--primer_file", help="BED format file containing primer scheme"
+    )
+    primer_file.add_argument(
+        "--primer_name",
+        help="Name of primer scheme (one word). Required if --primer_file is used",
+    )
+    primer_file.add_argument(
+        "--primer_description",
+        help="Description of primer scheme. Required if --primer_file is used",
+    )
+    artic = parser.add_argument_group()
+    artic.add_argument(
+        "--artic_primers",
+        action=SplitArgs,
+        help="Comma separated list of primers to fetch",
+    )
+    parser.add_argument(
+        "galaxy_datamanager_filename",
+        help="Galaxy JSON format file describing data manager inputs",
+    )
+    args = parser.parse_args()
+
+    if args.artic_primers is None and args.primer_file is None:
+        print(
+            "One of --artic_primers or --primer_file + --primer_name + --primer_description is required.",
+            file=sys.stderr,
+        )
+        exit(1)
+    elif args.primer_file is not None and (
+        args.primer_name is None or args.primer_description is None
+    ):
+        print(
+            "If --primer_file is used --primer_name and --primer_description is also required",
+            file=sys.stderr,
+        )
+        exit(1)
+    elif args.primer_file is not None and args.artic_primers is not None:
+        print(
+            "Only one of --artic_primers or --primer_file + --primer_name + --primer_description can be chosen"
+        )
+        exit(1)
+
+    config = json.load(open(args.galaxy_datamanager_filename))
+    output_directory = config.get("output_data", [{}])[0].get("extra_files_path", None)
+    if output_directory is None:
+        output_directory = args.output_directory
+
+    if not os.path.isdir(output_directory):
+        os.makedirs(output_directory)
+
+    data_manager_dict = {}
+    data_manager_dict["data_tables"] = json.load(
+        open(args.galaxy_datamanager_filename)
+    ).get("data_tables", {})
+    data_manager_dict["data_tables"] = data_manager_dict.get("data_tables", {})
+    data_manager_dict["data_tables"][DATA_TABLE_NAME] = data_manager_dict[
+        "data_tables"
+    ].get(DATA_TABLE_NAME, [])
+
+    if args.artic_primers:
+        data = fetch_artic_primers(output_directory, args.artic_primers)
+    else:
+        data = install_primer_file(
+            output_directory,
+            args.primer_file,
+            args.primer_name,
+            args.primer_description,
+        )
+
+    data_manager_dict["data_tables"][DATA_TABLE_NAME].extend(data)
+    print(data_manager_dict)
+    json.dump(data_manager_dict, open(args.galaxy_datamanager_filename, "w"))
b
diff -r 000000000000 -r ae730ecdc7e7 data_manager/install_primer_scheme_bedfiles.xml
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/data_manager/install_primer_scheme_bedfiles.xml Sun Apr 19 09:22:19 2020 -0400
b
@@ -0,0 +1,64 @@
+<tool id="data_manager_primer_scheme_bedfiles" name="BED-format primer scheme data manager" version="0.0.8" tool_type="manage_data" profile="19.05">
+    <requirements>
+        <requirement type="package">python</requirement>
+        <requirement type="package" version="2.22.0">requests</requirement>
+    </requirements>
+    <!-- fetch all the primers in one go -->
+    <command detect_errors="exit_code">
+    python '$__tool_directory__/install_primer_scheme_bedfiles.py'
+        '${output_file}'
+        #if $input.input_type == "ARTIC"
+            --artic_primers '${input.primers}'
+        #else 
+            --primer_file '${input.primer_input}'
+            --primer_name '${input.primer_name}'
+            --primer_description '${input.primer_description}'
+        #end if
+    </command>
+    <inputs>
+        <conditional name="input">
+            <param name="input_type" label="Choose the source for primer schemes" type="select">
+                <option value="ARTIC" selected="true">ARTIC SARS-CoV-2 Github page</option>
+                <option value="history">History</option>
+            </param>
+            <when value="ARTIC">                    
+                <param name="primers" type="select" multiple="true" label="SARS-CoV-2 Primers to fetch">
+                    <option value="ARTICv1" selected="true">ARTIC v1</option>
+                    <option value="ARTICv2" selected="true">ARTIC v2</option>
+                    <option value="ARTICv3" selected="true">ARTIC v3</option>
+                </param>
+            </when>
+            <when value="history">
+                <param name="primer_input" label="Select history item" type="data" format="bed" />
+                <param name="primer_name" label="Name for this primer scheme" type="text" 
+                       help="Non-word characters will be removed from primer scheme name and spaces replaced with underscore (_)" />
+                <param name="primer_description" label="Description for primer scheme" type="text" />
+            </when>
+        </conditional>
+    </inputs>
+    <outputs>
+        <data name="output_file" format="data_manager_json"/>
+    </outputs>
+    <tests>
+        <test>
+            <param name="input_type" value="ARTIC" />
+            <param name="primers" value="ARTICv1,ARTICv2,ARTICv3"/>
+            <output name="output_file">
+                <assert_contents>
+                    <has_text text="ARTIC"/>
+                </assert_contents>
+            </output>        
+        </test>
+        <test>
+            <param name="input_type" value="history" />
+            <param name="primer_input" ftype="bed" value="sample1.bed" />
+            <param name="primer_name" value="sample_primer" />
+            <param name="primer_description" value="sample primer scheme" />
+            <output name="output_file">
+                <assert_contents>
+                    <has_text text="sample_primer"/>
+                </assert_contents>
+            </output>        
+        </test>
+    </tests>
+</tool>
\ No newline at end of file
b
diff -r 000000000000 -r ae730ecdc7e7 data_manager/test-data/sample1.bed
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/data_manager/test-data/sample1.bed Sun Apr 19 09:22:19 2020 -0400
b
@@ -0,0 +1,7 @@
+MN908947.3      30      54      nCoV-2019_1_LEFT        60      +
+MN908947.3      385     410     nCoV-2019_1_RIGHT       60      -
+MN908947.3      320     342     nCoV-2019_2_LEFT        60      +
+MN908947.3      704     726     nCoV-2019_2_RIGHT       60      -
+MN908947.3      642     664     nCoV-2019_3_LEFT        60      +
+MN908947.3      1004    1028    nCoV-2019_3_RIGHT       60      -
+MN908947.3      943     965     nCoV-2019_4_LEFT        60      +
b
diff -r 000000000000 -r ae730ecdc7e7 data_manager_conf.xml
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/data_manager_conf.xml Sun Apr 19 09:22:19 2020 -0400
b
@@ -0,0 +1,20 @@
+<?xml version="1.0"?>
+<data_managers>    
+    <data_manager tool_file="data_manager/install_primer_scheme_bedfiles.xml" id="data_manager_primer_scheme_bedfiles">
+        <data_table name="primer_scheme_bedfiles">
+            <output>
+                <column name="value" />
+                <column name="description" />
+                <column name="path" output_ref="output_file" >
+                    <!-- note: the Python script sanitises the possibly user-supplied scheme name ('value') -->
+                    <move type="file">
+                        <source>${path}</source>
+                        <target base="${GALAXY_DATA_MANAGER_DATA_PATH}">primer_scheme_bedfiles/#echo str($value)#.bed</target>
+                    </move>
+                    <value_translation>${GALAXY_DATA_MANAGER_DATA_PATH}/primer_scheme_bedfiles/#echo str($value)#.bed</value_translation>
+                    <value_translation type="function">abspath</value_translation>
+                </column>
+            </output>
+        </data_table>
+    </data_manager>    
+</data_managers>
b
diff -r 000000000000 -r ae730ecdc7e7 tool-data/primer_scheme_bedfiles.loc.sample
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/tool-data/primer_scheme_bedfiles.loc.sample Sun Apr 19 09:22:19 2020 -0400
b
@@ -0,0 +1,8 @@
+# this is a tab separated file describing the location of primer schemes used for amplicon
+# sequencing (e.g. using the ARTIC SARS-CoV-2 protocol)
+#
+# the columns are:
+# value  description path
+#
+# for example
+# ARTICv1   ARTIC v1 primers    /data/galaxy/tool_data/artic_primers/ARTICv1.bed
\ No newline at end of file
b
diff -r 000000000000 -r ae730ecdc7e7 tool_data_table_conf.xml.sample
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/tool_data_table_conf.xml.sample Sun Apr 19 09:22:19 2020 -0400
b
@@ -0,0 +1,7 @@
+<tables>
+    <!-- Locations of primer scheme BED files -->
+    <table name="primer_scheme_bedfiles" comment_char="#">
+        <columns>value, description, path</columns>
+        <file path="tool-data/primer_scheme_bedfiles.loc" />
+    </table>
+</tables>