Next changeset 1:5e2a28bee02d (2018-02-17) |
Commit message:
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mageck commit 71cef018eec5ee7ff7f3853599c027e80e2637fe |
added:
mageck_macros.xml mageck_test.xml test-data/.Rapp.history test-data/demo/demo1/sample.txt test-data/demo/demo2/library.txt test-data/demo/demo2/test1.fastq test-data/demo/demo2/test2.fastq test-data/in.mageckQC.gmt test-data/in.mle.design_matrix.txt test-data/out.count.bam.txt test-data/out.count.fastq.txt test-data/out.count.log.txt test-data/out.count.unmapped.txt test-data/out.countsummary.pdf test-data/out.countsummary.txt test-data/out.mageckGSEA.txt test-data/out.mle.gene_summary.txt test-data/out.mle.log.txt test-data/out.mle.sgrna_summary.txt test-data/out.pathway.log.txt test-data/out.pathway.pathway_summary.txt test-data/out.test.R test-data/out.test.gene_summary.txt test-data/out.test.log.txt test-data/out.test.normalized.txt test-data/out.test.pdf test-data/out.test.sgrna_summary.txt test-data/test1.bam test-data/test1.fastq.gz |
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diff -r 000000000000 -r b8da4d41aa1d mageck_macros.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/mageck_macros.xml Wed Feb 14 06:42:55 2018 -0500 |
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@@ -0,0 +1,27 @@ +<?xml version="1.0"?> +<macros> + <token name="@VERSION@">0.5.7</token> + <xml name="requirements"> + <requirements> + <requirement type="package" version="@VERSION@">mageck</requirement> + <!-- needed for pdf report outputs --> + <requirement type="package" version="3.0.1">r-gplots</requirement> + <requirement type="package" version="1.8_2">r-xtable</requirement> + <yield/> + </requirements> + </xml> + <xml name="version"> + <version_command>mageck -v </version_command> + </xml> + <xml name="citations"> + <citations> + <citation type="doi">10.1186/s13059-014-0554-4</citation> + </citations> + </xml> + <xml name="sort_criteria"> + <param name="sort_criteria" argument="--sort-criteria" type="select" label="Sorting criteria"> + <option value="neg" selected="True">Negative selection</option> + <option value="pos">positive selection</option> + </param> + </xml> +</macros> |
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diff -r 000000000000 -r b8da4d41aa1d mageck_test.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/mageck_test.xml Wed Feb 14 06:42:55 2018 -0500 |
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b'@@ -0,0 +1,281 @@\n+<?xml version="1.0"?>\n+<tool id="mageck_test" name="MAGeCKs test" version="@VERSION@" >\n+ <description>- given a table of read counts, perform the sgRNA and gene ranking</description>\n+ <macros>\n+ <import>mageck_macros.xml</import>\n+ </macros>\n+ <expand macro="requirements" />\n+ <expand macro="version" />\n+ <command detect_errors="exit_code"><![CDATA[\n+\n+mageck test\n+-k \'$count_table\'\n+\n+#if $sampleids.sample_select == "treated":\n+ -t \'$sampleids.treatment_id\'\n+#elif $sampleids.sample_select == "control":\n+ --day0-label \'$sampleids.day0_label\'\n+#end if\n+\n+#if \'$control_id\':\n+ -c \'$control_id\'\n+#end if\n+\n+-n output\n+\n+$out.normcounts\n+$out.pdfreport\n+\n+--norm-method $adv.norm_method\n+--gene-test-fdr-threshold $adv.fdr_threshold\n+--adjust-method $adv.adjust_method\n+$adv.var_samples\n+--sort-criteria $adv.sort_criteria\n+--remove-zero $adv.remove_zero\n+--gene-lfc-method $adv.lfc_method\n+#if $adv.control_sgrna:\n+ --control-sgrna $adv.control_sgrna\n+#end if\n+#if $adv.cnv_norm:\n+ --cnv-norm $adv.cnv_norm\n+#end if\n+#if $adv.cell_line:\n+ --cell-line $adv.cell_line\n+#end if\n+\n+ ]]></command>\n+ <inputs>\n+ <param name="count_table" argument="--count-table" type="data" format="tabular" label="Counts file" help="A tab-separated file of read counts. See Help below for format" />\n+ <conditional name="sampleids">\n+ <param name="sample_select" type="select" label="Specify Treated samples or Control"\n+ help="You can choose to either specify the treated samples or the control">\n+ <option value="treated">Treated samples</option>\n+ <option value="control">Control sample</option>\n+ </param>\n+ <when value="treated">\n+ <param name="treatment_id" argument="--treatment-id" type="text" label="Treated Sample Labels (or Indexes)" help="If sample label is provided, the labels must match the labels in the first line of the count table, separated by comma (,); for example, HL60.final,KBM7.final. For sample index, 0,2 means the 1st and 3rd samples are treatment experiments. See Help below for a detailed description." />\n+ </when>\n+ <when value="control">\n+ <param name="day0_label" argument="--day0-label" type="text" optional="true" value="" label="Control Sample Label" help="Specify the label for the control sample. For every other sample label, the module will treat it as a treatment condition and compare with control sample (usually day 0 or plasmid)" />\n+ </when>\n+ </conditional>\n+ <param name="control_id" argument="--control-id" type="text" optional="true" label="Control Sample Labels (or Indexes)" help="If sample label is provided, the labels must match the labels in the first line of the count table, separated by comma (,). Default is all the samples not specified in treatment experiments. See Help below for a detailed description." />\n+\n+ <section name="out" title="Output Options">\n+ <param name="normcounts" argument="--normcounts-to-file" type="boolean" truevalue="--normcounts-to-file" falsevalue="" checked="false" optional="true" label="Output normalized counts file" help="Default: No" />\n+ <param name="pdfreport" argument="--pdf-report" type="boolean" truevalue="--pdf-report" falsevalue="" checked="false" optional="true" label="Output PDF report" help="Generate pdf report of the input file. Default: No" />\n+ <param name="rscriptOpt" type="boolean" truevalue="True" falsevalue="" checked="false" optional="true" label="Output R script" help="Output the R script used to generate the plots in the pdf report. Default: No" />\n+ <param name="out_log" type="boolean" truevalue="True" falsevalue="" checked="false" label="Output logfile" help="This file includes the logging information during the execution. Default: No" />\n+ </section>\n+\n+ <section name="adv" title="Ad'..b'---- ----------- ------------ ----------------- ----------- ------------- --------------- ----------- ------------ ----------------- -----------\n+ESPL1 12 6.4327e-10 7.558e-06 7.9e-05 1 -2.35 11 0.99725 0.99981 0.999992 615 0 -0.07\n+RPL18 12 6.4671e-10 7.558e-06 7.9e-05 2 -2.12 11 0.99799 0.99989 0.999992 620 0 -0.32\n+CDK1 12 2.6439e-09 7.558e-06 7.9e-05 3 -1.93 12 1.0 0.99999 0.999992 655 0 -0.12\n+======= ======= ============= =============== =========== ============ ================= =========== ============= =============== =========== ============ ================= ===========\n+\n+The contents of each column is as follows:\n+\n+* **id** Gene ID\n+* **num** The number of targeting sgRNAs for each gene\n+* **neg|score** The RRA lo value of this gene in negative selection\n+* **neg|p-value** The raw p-value (using permutation) of this gene in negative selection\n+* **neg|fdr** The false discovery rate of this gene in negative selection\n+* **neg|rank** The ranking of this gene in negative selection\n+* **neg|goodsgrna** The number of "good" sgRNAs, i.e., sgRNAs whose ranking is below the alpha cutoff (determined by the --gene-test-fdr-threshold option), in negative selection.\n+* **neg|lfc** The log fold change of this gene in negative selection\n+* **pos|score** The number of targeting sgRNAs for each gene in positive selection (usually the same as num.neg)\n+* **pos|score** The RRA lo value of this gene in negative selection\n+* **pos|p-value** The raw p-value of this gene in positive selection\n+* **pos|fdr** The false discovery rate of this gene in positive selection\n+* **pos|rank** The ranking of this gene in positive selection\n+* **pos|goodsgrna** The number of "good" sgRNAs, i.e., sgRNAs whose ranking is below the alpha cutoff (determined by the --gene-test-fdr-threshold option), in positive selection.\n+* **pos|lfc** The log fold change of this gene in positive selection\n+\n+Genes are ranked by the p.neg field (by default). If you need a ranking by the p.pos, you can use the --sort-criteria option.\n+\n+-----\n+\n+**More Information**\n+\n+**Overview of the MAGeCK algorithm**\n+\n+Briefly, read counts from different samples are first median-normalized to adjust for the effect of library sizes and read count distributions. Then the variance of read counts is estimated by sharing information across features, and a negative binomial (NB) model is used to test whether sgRNA abundance differs significantly between treatments and controls. This approach is similar to those used for differential RNA-Seq analysis. We rank sgRNAs based on P-values calculated from the NB model, and use a modified robust ranking aggregation (RRA) algorithm named \xce\xb1-RRA to identify positively or negatively selected genes. More specifically, \xce\xb1-RRA assumes that if a gene has no effect on selection, then sgRNAs targeting this gene should be uniformly distributed across the ranked list of all the sgRNAs. \xce\xb1-RRA ranks genes by comparing the skew in rankings to the uniform null model, and prioritizes genes whose sgRNA rankings are consistently higher than expected. \xce\xb1-RRA calculates the statistical significance of the skew by permutation, and a detailed description of the algorithm is presented in the Materials and methods section of the `MAGeCK paper`_. Finally, MAGeCK reports positively and negatively selected pathways by applying \xce\xb1-RRA to the rankings of genes in a pathway.\n+\n+For more information on using MAGeCK, see the `MAGeCK website here`_.\n+\n+.. _`MAGeCK paper`: https://genomebiology.biomedcentral.com/articles/10.1186/s13059-014-0554-4\n+.. _`MAGeCK website here`: https://sourceforge.net/p/mageck/wiki/QA/#using-mageck\n+\n+ ]]></help>\n+ <expand macro="citations" />\n+</tool>\n' |
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diff -r 000000000000 -r b8da4d41aa1d test-data/demo/demo1/sample.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/demo/demo1/sample.txt Wed Feb 14 06:42:55 2018 -0500 |
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|
b |
diff -r 000000000000 -r b8da4d41aa1d test-data/demo/demo2/library.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/demo/demo2/library.txt Wed Feb 14 06:42:55 2018 -0500 |
b |
b'@@ -0,0 +1,2550 @@\n+s_10007\tTGTTCACAGTATAGTTTGCC\tCCNA1\n+s_10008\tTTCTCCCTAATTGCTTGCTG\tCCNA1\n+s_10027\tACATGTTGCTTCCCCTTGCA\tCCNC\n+s_10035\tAGAGACCAGCCCGCTGACCG\tCCND2\n+s_10164\tGCAGGCGGTACTCAAGGGCA\tCCS\n+s_10200\tTTAGAGAAGATCCATCATTC\tCCT7\n+s_10232\tAACACGACAGACTTCTGTTC\tCD164\n+s_10264\tGAGTCACAGGACGCCCTGAT\tCD1D\n+s_10340\tCACGGCTCTGTCACCATCAC\tCD276\n+s_1035\tACACTTGTCATCCGCCTTCA\tADAMTS14\n+s_10381\tTCTTTCAGCTCAACAGGCCA\tCD300LG\n+s_10387\tACCACAGCCGAGCTCGTCGC\tCD320\n+s_10401\tCCTCGAGTAGGCCGACGCCC\tCD37\n+s_10402\tCCTGGGCGTCGGCCTACTCG\tCD37\n+s_10405\tTCAGACCGTACCTTGCAACA\tCD38\n+s_10408\tCCGACACACAAGCTCTGTTG\tCD3D\n+s_10417\tCAGAGACTGACATGGAACAG\tCD3G\n+s_10425\tCTGCACCGCTCATGCTCGCC\tCD40\n+s_10434\tTGAAGCAATATGTGTCATAC\tCD44\n+s_10437\tGAGAAACATGTCCATATATA\tCD46\n+s_10448\tCATACCAGCTGAGCCGTCCG\tCD5\n+s_10461\tAATGGTCAGATTGATGTACC\tCD55\n+s_10481\tTTGAGCTGGTCAGGTGGGGC\tCD6\n+s_10518\tCATCTACATCCTCATCGCTG\tCD81\n+s_10541\tGGCGCGCCACGATGCGGCCG\tCD8B\n+s_10563\tTTTCTTTCACTGCATCCTCC\tCD99L2\n+s_10582\tTAAAGCTTCTACGCAGATTT\tCDC14B\n+s_10594\tTCTCTTCTGCTTCAAGTCTT\tCDC20B\n+s_10615\tTTGCTTTACTCTTTCATTGT\tCDC27\n+s_10616\tGCGCTCACCTCGTAGATGTT\tCDC34\n+s_1064\tACATGGCGAGAGGTGCTCAA\tADAMTS4\n+s_10657\tAGCTGTATGCACAAAGTTTG\tCDC42SE2\n+s_10658\tCAATGTCCAGATGCAGCTCG\tCDC42SE2\n+s_10660\tTCGTATCCACGAGCTGCATC\tCDC42SE2\n+s_10675\tCCAAGTAGATCTTTTCGATC\tCDC73\n+s_10731\tACTCACCACTGTCAATTGCC\tCDH13\n+s_10739\tGCTTTGTGTCTCCGTCCCCC\tCDH16\n+s_10770\tTGGACTACGAGGAGGTGCAC\tCDH23\n+s_10785\tTCTACAGCGAAGACACCCTC\tCDH3\n+s_1085\tGACGTAGCAGCATTAAACAG\tADAMTS9\n+s_10862\tGGTGAGAGTGACCAAGCTTC\tCDK17\n+s_10943\tGCATTTAAGTACAACTCCAT\tCDKL5\n+s_10946\tTTCATGAAGATTCCTAACAT\tCDKL5\n+s_1101\tTCCTGGCCGCTCGAATCTCC\tADAMTSL4\n+s_11025\tGGGCCCATCGTCTCGCACTC\tCDSN\n+s_11035\tCAACCCCACAGGTGAAGATC\tCDX1\n+s_11054\tCAAATTGTAGGATATGCAAT\tCEACAM1\n+s_11055\tCATGCCATTCAATGTTGCAG\tCEACAM1\n+s_11095\tCGAGTACGGCTACGTGAGCC\tCEBPB\n+s_1113\tGCTTGAGTGTATGCACAAAT\tADAR\n+s_11153\tAGCCTGTCCGTTTGCTAACG\tCELF4\n+s_1116\tAGCTGACGCTGTCTCACGCC\tADARB1\n+s_11211\tGAAGACGTTCTCATAACATT\tCENPK\n+s_11218\tTTCTTTCGCAATACATCCAG\tCENPL\n+s_11222\tCCAAGATGGTGGCCGTGTTC\tCENPM\n+s_1123\tCCTCAGCATGGCTATCATTG\tADAT1\n+s_11251\tTTTGTAGTCGTCAGCTCATC\tCEP104\n+s_1127\tGTCTACAACAATGAAGTTGT\tADAT2\n+s_11294\tAGTTACAACTTTTGCCCAGC\tCEP41\n+s_11296\tCCAGTCTTGATTTGATATGC\tCEP41\n+s_11317\tACAGCTGAGCCAAGAACTAA\tCEP63\n+s_1136\tTCCCATACACGCCCTCATCA\tADC\n+s_11386\tTGCCGGCAATGAAGGCAATC\tCERS2\n+s_11438\tTTTTTTTACATCAAACCCCA\tCETN3\n+s_11456\tCATCCTGGAGATACTCCTTT\tCFH\n+s_11513\tACGTGCCTGAGGGCTACTCC\tCGREF1\n+s_11527\tAGCCGCGCACGAAGCCCACA\tCHAC1\n+s_11585\tAGCCAGTCGGATGATGATTC\tCHD1\n+s_11598\tTTACACGCTTCTTGCGTTTT\tCHD3\n+s_1161\tACCTTAACTGCTCTCTGATC\tADCY10\n+s_11692\tTGAGATATGTGCCCCTCGTT\tCHL1\n+s_11714\tCTCATGGTGGCCTGAATCTC\tCHMP3\n+s_11744\tTTTCAGGTGCATACATTCAG\tCHN1\n+s_1177\tTGAGCAGCTTCACAAGCTCC\tADCY3\n+s_11788\tCGTGAACTGCATCTGCTCAG\tCHRDL1\n+s_1179\tCTCGTAGAAGAGGTCTTCGC\tADCY4\n+s_118\tTTACAAGTTGGTTTTGCAGT\tABCA12\n+s_1183\tCTGCACCCACTATCCGGCTG\tADCY5\n+s_11837\tTTCTAATGCTCTTCGTCCAG\tCHRNA9\n+s_11862\tTGGGGTCGTAGTTTTGCATC\tCHRNG\n+s_11877\tCCTGCAGAAGCTCTATGACC\tCHST13\n+s_11928\tACGTCTGTCTGTACCAGCAT\tCHUK\n+s_11984\tATGTCTTACCAACCCATCCA\tCINP\n+s_11988\tTACCTGTGATGGATCATTTG\tCIR1\n+s_12015\tTACCCTGGGGTAGCCTCAAA\tCITED1\n+s_12019\tCTGGGCAGGGCGGTAAATCT\tCIZ1\n+s_12021\tTCCTACAGGTGTGGACTTCC\tCIZ1\n+s_12049\tCCTTTCAGTAGTTGCCATCT\tCKLF-CMTM1\n+s_12057\tCCGGCAGAAAGTGTGTGCCG\tCKMT2\n+s_12069\tCTGTATCTGCTTGAAGCTAC\tCLCA1\n+s_12082\tGTCAAAGCAATCCTGTTTTT\tCLCC1\n+s_12089\tTCCCCTGTGCGATTGAGAGC\tCLCF1\n+s_1212\tGACCAGAGTGAACTCCGAGC\tADD1\n+s_12120\tGCTCCTGTCCCTCAAGTATG\tCLCN7\n+s_12121\tGGACGCCCACCTCATACTTG\tCLCN7\n+s_12138\tACATGTCCAGGGCGCAGATC\tCLDN10\n+s_12140\tTTAGGGCTGTGCTCAATGAC\tCLDN10\n+s_12160\tTCAGAACGAAGATGCCCGTC\tCLDN17\n+s_12177\tACGGGACTTCTACTCACCAC\tCLDN2\n+s_12187\tGCATCCCTGACATCATACCT\tCLDN25\n+s_12197\tGTCTCCAGGTCTTGCCGCCT\tCLDN7\n+s_12203\tCACACTAACTGAGCAGTTCA\tCLDND1\n+s_12209\tCGTGGTTGCATTCCTGCCAC\tCLDND2\n+s_1224\tTGAAGTCCGCGATCTGTCGC\tADD2\n+s_12262\tTGACAGGACCGCGCAGTTCT\tCLEC3B\n+s_12310\tGCACAGCTTCTGCACTGTCT\tCLIC1\n+s_1238\tCTTCAACCTCTTCAATGCAA\tADH4\n+s_12420\tGAACAGCACGATCTTGTGTA\tCLPTM1\n+s_12425\tCGCATTCATATGTCAGTTTT\tCLPX\n+s_12433\tCATCTGCCTATGGAATGTCC\tCLRN2\n+s_12463\tTCTCTTTCCAGCTGGTTCTG'..b's_7053\tTTCTCACCGGCAAGACCTAG\tC1QTNF7\n+s_7059\tAGAGCTCACGTAAGCATCTC\tC1QTNF9B\n+s_707\tCGTTTCAGTCCACCTGATCC\tACPL2\n+s_7088\tCTGAACACTCACCAGACTGC\tC1orf106\n+s_7105\tAGTACCAAAGCATCATTGTC\tC1orf111\n+s_7128\tCTGCCGTAACATCTCCTCGC\tC1orf122\n+s_714\tCGCTTCTCACCGTGGTCAGC\tACPT\n+s_7144\tATTTGACCTTACTCTTATAC\tC1orf130\n+s_7162\tCCACACTCACCTGTCGAGGA\tC1orf144\n+s_7217\tTACCCATGTGCCTGCTGAGC\tC1orf186\n+s_7234\tCCAACTTACCTTTGTTTCCC\tC1orf198\n+s_7252\tCACATTCCCTGTTGCCTTCG\tC1orf212\n+s_730\tTTGACTCAGCATCACGCCCT\tACSBG1\n+s_7306\tGTCCTGTGTCACTCCTGCTT\tC1orf49\n+s_7336\tCTTGTGCTGCCTGGAGGACG\tC1orf61\n+s_7341\tTCAGCCAAAGAAACAAGCCG\tC1orf63\n+s_7465\tCATTCCAGTTGGGGTAGTCC\tC20orf26\n+s_747\tTCTTCCGACCAACACGCTTA\tACSL1\n+s_7498\tCTCCTGGGTCTTCTCAATCT\tC20orf96\n+s_7539\tCACTCGCCTTACCTCGCCCT\tC22orf25\n+s_7562\tTCATGGCAGGGTCCCACATC\tC22orf42\n+s_76\tCGTGGATCCACGAAGCGAGA\tAARS2\n+s_7626\tGTAGCTAATGCTGCCTACCT\tC2orf43\n+s_7629\tCAAGCAGTGCATCCGATCCA\tC2orf44\n+s_7674\tGGTGTGGTCCGAGACCACCA\tC2orf57\n+s_7681\tGGCCCACAGCATGTCCTCCA\tC2orf62\n+s_7692\tTAGATCACATCAAGCAGTGT\tC2orf63\n+s_77\tACCCACCTTGATATCCTCCT\tAARSD1\n+s_7715\tCCTTACCCGGGCATGAGTCC\tC2orf70\n+s_7793\tTACTTCTGTGCAGGCCATGC\tC3orf18\n+s_7794\tTCTTGCAGGTTTTGTACATC\tC3orf18\n+s_78\tAGGAAAAGGTGGCCTGGCCG\tAARSD1\n+s_7803\tCCAGACGGTGATGTAAAATC\tC3orf22\n+s_781\tCCGAGAGCGTTGCTTTGATC\tACSS1\n+s_782\tCGGGCATGTAGATGGCAACA\tACSS1\n+s_7822\tACTCAGCGTCTCCCGCCTCC\tC3orf27\n+s_7838\tCGTGAGAAGAGACACCTGTG\tC3orf35\n+s_786\tCACATGTTGACTCCCCTTCC\tACSS2\n+s_791\tGTTGCTTTAGTGTTTGTAAC\tACSS3\n+s_7927\tACCCATGGCTCCGATGGAGA\tC4orf19\n+s_7940\tTGTGCAAATTAATATGCTGC\tC4orf26\n+s_7970\tTCTGAAACATTATAGTGTCC\tC4orf37\n+s_8000\tCTGCGCACCGCGCTCATTTT\tC4orf52\n+s_804\tCGTACAGGGATAGCACAGCC\tACTB\n+s_8073\tTCTTCTAGAACTTTACACAC\tC5orf51\n+s_8141\tTGAGCCTCCAACATTCAAAA\tC6orf162\n+s_8148\tTCCATGTTAAAGCCATTACT\tC6orf165\n+s_82\tCTATGCCAAAGTGAACTCCA\tAARSD1\n+s_8227\tTATGAACAATATAAAGCCAT\tC7orf10\n+s_825\tTGCCAAGACCTCGTAACCTG\tACTL6A\n+s_8281\tCTGCACCTTCACTCAGTACC\tC7orf59\n+s_8318\tTTCTCCTGTGCAGTTACCCC\tC8A\n+s_8403\tAGGTTACCTGCAGGGTGGTC\tC9orf100\n+s_8419\tACCGGGAGGCGCAGTGATAT\tC9orf123\n+s_8470\tCCGCGGATCACGCCCCGATG\tC9orf24\n+s_8476\tGACCATAGTGCTTTTCCTCG\tC9orf3\n+s_8498\tGCTCCAGCAGACACCTATTC\tC9orf50\n+s_8514\tGGGCTCCATATTCGACTGTC\tC9orf71\n+s_8592\tGGTGCGTCCTCGGAGATCAG\tCA6\n+s_8699\tGTTTGAGCGCATCAGCATGT\tCACNA1G\n+s_8705\tCCGGGGTCCGGGCTGCTCCG\tCACNA1I\n+s_871\tCGAACCTGTTCAGGGGACAC\tACTR8\n+s_8738\tCCCGTCCGCGGTCCCGTTCT\tCACNB4\n+s_874\tTTGCTTGATCCACCATTTTA\tACTR8\n+s_8757\tCCAGCTGACATCCGAGTCCA\tCACNG5\n+s_8795\tCGCTGTGCAGAGTTACTATG\tCADPS\n+s_8797\tGCTGTTCCGCCAACGACTCC\tCADPS\n+s_8848\tGGCGCAGTACCGCGCCAATG\tCALHM2\n+s_8879\tGCCGCACAATCAGTGTGTAC\tCALR\n+s_8880\tTCTTCCGGTTTTGAAGCATC\tCALR\n+s_8907\tCTGCTGAAGCACCCCAACAT\tCAMK2A\n+s_8910\tACCTCTGGCTGACAGCTTCT\tCAMK2B\n+s_8917\tTTTTGCTGCGTACTCCTGCG\tCAMK2G\n+s_8918\tACCAACAACTTCTTCGGCGC\tCAMK2N1\n+s_893\tTGCCCACGATCTCCATGTGC\tACVR1B\n+s_8930\tTCTCACTCTGCAGCTTGTAC\tCAMKK1\n+s_8939\tCCTTGCGGCCGTCCCGCTTC\tCAMKV\n+s_8954\tCCCTTTACCTTTTGATGAGC\tCAMSAP1\n+s_9038\tTTTCGATGAAACTTTGATCA\tCAPN3\n+s_906\tTGCCACTTATGTTAATTGCG\tACVR2A\n+s_9064\tGGATGCCGTTTCTAAGTACC\tCAPRIN1\n+s_9077\tAAGACTCCGACTGCAGTGCC\tCAPSL\n+s_9109\tTCAAATAGTACTTCCTTCCT\tCARD17\n+s_9124\tTCCTTGTAGTCTAATGAGTT\tCARD8\n+s_9126\tTTACGTATCATTTGTCACAC\tCARD8\n+s_913\tCTCACTATAAAAGACAGTGA\tACY1\n+s_9161\tTGTTTCAATTTCTCTGCTTC\tCASC1\n+s_9171\tCAAGACTGCATCAGTTAAAG\tCASD1\n+s_9196\tGCACCTCAACTGTACCAAAG\tCASP10\n+s_9221\tTCCTGCAGCTCATCCGAATA\tCASP4\n+s_9223\tCGTCAACCACAGTGTAGCCC\tCASP5\n+s_9257\tCACTTGCAACACCGTTTCTA\tCASR\n+s_9285\tCGATAAGCTTGAGGATGCGC\tCATSPER3\n+s_9506\tTCTCCTACCTTCAATGCCCC\tCCDC120\n+s_9507\tCAGTTCAGGGCTGGACCAGC\tCCDC121\n+s_9517\tCACTGCCTGCGTATGACTTG\tCCDC125\n+s_952\tTTGATTCAGGGGTGAGCTTA\tADAM12\n+s_9584\tCATCACTTCCTCCTGAAAAA\tCCDC149\n+s_964\tTCATTTGACTTAATCTTCCG\tADAM18\n+s_9646\tTTCAGCTTTCAGTTCTTGAC\tCCDC170\n+s_9678\tCTCATAGACATCAGTCACCT\tCCDC28B\n+s_9710\tACACTTTTTGCGCTCCTCGT\tCCDC40\n+s_9725\tCTCCAACCGTCCGTCCAGCC\tCCDC43\n+s_9732\tCCTGCACCGCGTGCGAGAGC\tCCDC48\n+s_976\tTCCATTGAGAGAAATCGTTC\tADAM21\n+s_9763\tTACGACAATGATAAAGCTAA\tCCDC62\n+s_9791\tACTCACCTGTGTCCCCATTG\tCCDC69\n+s_9868\tCCTTCTCGCGCGCCTGTGCC\tCCDC89\n+s_991\tATCGCCCCTCTGAGCAGTCC\tADAM30\n+s_9925\tGCCTGCGCCTTGGACACAGT\tCCL1\n+s_9973\tATACTCACATCACAGCCCGC\tCCL26\n' |
b |
diff -r 000000000000 -r b8da4d41aa1d test-data/demo/demo2/test1.fastq --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/demo/demo2/test1.fastq Wed Feb 14 06:42:55 2018 -0500 |
b |
b'@@ -0,0 +1,10000 @@\n+@MISEQ:152:000000000-A8HE7:1:1101:14429:1925 1:N:0:ANATCG\n+CAGAAATACAGTGCGACCTGTTTTAGAGCT\n++\n+AAA?11DFF11@1FEGGC0FDFHFF0B1D1\n+@MISEQ:152:000000000-A8HE7:1:1101:12740:1966 1:N:0:ANATCA\n+CTCCACTTCACTGTCAGTTGGTTTTAGAGC\n++\n+1>AAACB1B@133BA33AB1BEHHAFFHHG\n+@MISEQ:152:000000000-A8HE7:1:1101:16950:1984 1:N:0:ANATCG\n+CCTACACGCACCTGGCGTCCGTTTTAGAGC\n++\n+>1>1>CF1?1ADGGGF0EEEGGHHGCEHFG\n+@MISEQ:152:000000000-A8HE7:1:1101:17242:1990 1:N:0:ANATCG\n+CGATCCACAATTTTAAAAGAAAAGGGGGGA\n++\n+>11AAAFFFAFFGF3FEGBFF110000AE#\n+@MISEQ:152:000000000-A8HE7:1:1101:14179:1995 1:N:0:ANATCG\n+ATCACATCTGTTTTAGAGCTAGAAATAGCA\n++\n+A3AABDFFFBDFGGFFFBGBGG4FEGHGBF\n+@MISEQ:152:000000000-A8HE7:1:1101:12622:2006 1:N:0:ANATCG\n+TGTTGCAAGGCTAGTCCGTTATCAACTTGA\n++\n+AAAA?FF5@3AFGAGGGGGGGGHEB4AAF4\n+@MISEQ:152:000000000-A8HE7:1:1101:16776:2010 1:N:0:ANATCG\n+GCAGACGTTGGCTAAGGCTAGTCCGATATC\n++\n+BBBBBF@A3AFFGGGF4GGBGGBBE22A2D\n+@MISEQ:152:000000000-A8HE7:1:1101:11913:2010 1:N:0:ANATCG\n+TATCATATCCCCATGTTTGCGTTTTAGAGC\n++\n+AA>>3@5BFDF@G4BEGGCGGGGGGEGFFH\n+@MISEQ:152:000000000-A8HE7:1:1101:13624:2011 1:N:0:ANATCG\n+CCAGAACGACCACGTCTGTTTAGAGCTAGC\n++\n+AA?1AFFA>ADDGGGGG0AB3331B1AGG1\n+@MISEQ:152:000000000-A8HE7:1:1101:17657:2029 1:N:0:ANATCG\n+TACCTTGGATCACTTACATCGTTTTAGAGC\n++\n+AA1AADF111B>111BGG3GA1BFFA0A11\n+@MISEQ:152:000000000-A8HE7:1:1101:16812:2029 1:N:0:ANATCG\n+CTTCAAGCTGTAATTCCGTAGTTTTAGAGC\n++\n+AAAAA13@1@1DG3FGGGGAEGGGBDBBDF\n+@MISEQ:152:000000000-A8HE7:1:1101:13595:2033 1:N:0:ANATCG\n+TCAGCATCTCCAGAGCTGCCGTTTTAGAGC\n++\n+1A1AAFFF11@1B1FG111BEFGHGHCABG\n+@MISEQ:152:000000000-A8HE7:1:1101:13444:2034 1:N:0:ANATCG\n+ACAGTTCTGTATGACATCATGTTTTAGAGC\n++\n+?A11AFF3D@@DBGG13B1BGGHHHGGGHH\n+@MISEQ:152:000000000-A8HE7:1:1101:12520:2041 1:N:0:ANCTCG\n+TGAAAGTACTAGAAAACGTGGCACCGAGTC\n++\n+A>1>AF@3BD3DFAGB11AAF00B00AA0A\n+@MISEQ:152:000000000-A8HE7:1:1101:13860:2049 1:N:0:ANATCG\n+CTCCGGCTGTGGAGGCTGTGGTTTTAGAGC\n++\n+BBBBB@BBBFFFGGGGGGFGGGGHGGHHHH\n+@MISEQ:152:000000000-A8HE7:1:1101:12559:2049 1:N:0:ANATCG\n+TGACATCGTAGACTCGCCCAGTTTTAGAGC\n++\n+A1AAA3F1>>AAF3F1ECE?CGHHHGFGFG\n+@MISEQ:152:000000000-A8HE7:1:1101:18030:2053 1:N:0:ANATCG\n+TCTCACCGTGCTGTCACATTTAGAGCTAGA\n++\n+AAAA?FFAC1AAFFFAFGGGGF311AFDG1\n+@MISEQ:152:000000000-A8HE7:1:1101:14400:2054 1:N:0:ANATCG\n+GGGCGATGCACCGTTAATCGTTTTAGAGCT\n++\n+A33AA?@AAFFFGGCEGGGGGGGGHCGBGG\n+@MISEQ:152:000000000-A8HE7:1:1101:17476:2054 1:N:0:ANATCG\n+CTGACGGGACCATGAAAATCGTTTTAGAGC\n++\n+1>>AA11A>>1AFGBGGFGGCFCFCHHFBA\n+@MISEQ:152:000000000-A8HE7:1:1101:11326:2062 1:N:0:ANATCG\n+CAGCTACTTCTTCCTTGCGCTTTTAGGGCT\n++\n+>>>>>@1FFFFFGFCG1E1E0EFH3010A0\n+@MISEQ:152:000000000-A8HE7:1:1101:15694:2064 1:N:0:ANATAG\n+TCCCCTGACGCCCGAGCAGCGTTTTAGAGC\n++\n+3>AAAFAFFAABGGGCGG4CAEEEFEBBEF\n+@MISEQ:152:000000000-A8HE7:1:1101:17231:2064 1:N:0:ANATCG\n+GTCCTCCCGATGACTGCCATGTTTTAGAGC\n++\n+A>>AAFFAA?D1GGEABGF1GGHGHBFE1D\n+@MISEQ:152:000000000-A8HE7:1:1101:11454:2072 1:N:0:ANATCG\n+CCTCTGACCTCAAGTTAAAATAAGGCTAGT\n++\n+1A1A11DDFFFFE1AFD3GD31BBGBCGEE\n+@MISEQ:152:000000000-A8HE7:1:1101:11841:2085 1:N:0:ANATCG\n+CGTCCCTCCTGACGCTCAGAGTTTTAGAGC\n++\n+AAAB@BFFBFFBF2AEFGGCGGGHHHHHHH\n+@MISEQ:152:000000000-A8HE7:1:1101:15365:2092 1:N:0:ANATCG\n+CCTACCAGGGCTGTTGATTAGTTTTAGAGC\n++\n+AAAAA>C1?1AFGFGFF3FFGGHH3FFF1A\n+@MISEQ:152:000000000-A8HE7:1:1101:18500:2095 1:N:0:ANATCG\n+CCCATGTGCCCAGATGCACTGTTTTAGAGC\n++\n+AAA??1BFF1F11A1F1F1FA3AF0A0BAA\n+@MISEQ:152:000000000-A8HE7:1:1101:11881:2096 1:N:0:ANATCG\n+GTACCTCTTAGTTGACATTTGTTTTAGAGC\n++\n+AA1>>FF1DDDBFGGFGCGGGGHHHHGHHH\n+@MISEQ:152:000000000-A8HE7:1:1101:14801:2113 1:N:0:ANATCG\n+GGCAGAGCGCACATTGTTCTGTTTTAGAGC\n++\n+>>AA33ACDADBGGGBFGGFGGHGHHHHHG\n+@MISEQ:152:000000000-A8HE7:1:1101:14238:2115 1:N:0:ANATCG\n+TCTCCAAAATAAGGCTAGTCCGTTATCAAC\n++\n+BBBBBFBF>FFFGGGFGCBGFEHHGAGFEF\n+@MISEQ:152:000000000-A8HE7:1:1101:15070:2115 1:N:0:ANATCG\n+TCCGCCGGGCGATCCGCCTGGTTTTAGAGC\n++\n+>1111111A>0>0FEFAE?A/FFCCEFC1F\n+@MISEQ:152:000000000-A8HE7:1:1101:17465:2116 1:N:0:ANATCG\n+CCATCCTGCCTTCAAGTACCGTTTTAGAGC\n++\n+AA3>AFFFFFFFGGCFFGGEEFGHGHHBGD\n+@MISEQ:152:000000000-A8HE7:1:1101:11476:2123 1:N:0:ANATCG\n+CGTGAAGACAGGT'..b'01:19674:3663 1:N:0:ANATAG\n+TGTACCCTGCCCACACTCCCGTTTTAGAGC\n++\n+A>A113@1BAFA1FE1B1FFGGHHG3A111\n+@MISEQ:152:000000000-A8HE7:1:1101:11709:3663 1:N:0:ANATCG\n+TGGCTCACCAGTACCCCTCTGTTTTAGAGC\n++\n+AAAA111111C@AEDAFFE1CFGHHHBFHH\n+@MISEQ:152:000000000-A8HE7:1:1101:14789:3664 1:N:0:ANATCG\n+AGTAGCAAAACATGTTACGTGTTTTAGAGC\n++\n+?AAAAFFFFFFAGB3BGGGAGFHHHBGFHG\n+@MISEQ:152:000000000-A8HE7:1:1101:10966:3664 1:N:0:ANATCG\n+GGGCTCCATATTCGACTGTCGTTTTAGAGC\n++\n+CCCCCCBFFFFFGGGGGGGGGFEFFHHGHH\n+@MISEQ:152:000000000-A8HE7:1:1101:20525:3665 1:N:0:ANATCG\n+CTCCTCGAAGGGCCGACACGTTTTAGAGCT\n++\n+>AA3AB33CA22AEE2EA2EBFGGHFBDFG\n+@MISEQ:152:000000000-A8HE7:1:1101:17351:3665 1:N:0:ANATCG\n+GAGCCCTACCCGCTCGTCTAGTTTTAGAGC\n++\n+BBBBBBFFCCFAE2EEEC4FFGFGFHHFHH\n+@MISEQ:152:000000000-A8HE7:1:1101:10622:3665 1:N:0:ANATCG\n+CGTATCGCCACCCTGGCATCGTTTTAGAGC\n++\n+AB>AAFBAABBFGCFCFFGGGFGGGHFFHH\n+@MISEQ:152:000000000-A8HE7:1:1101:11162:3666 1:N:0:ANATCG\n+TGTGTACAGCTCTCCTCCGTTTTAGAGCTA\n++\n+ABAAAFFFF4CFGGGBGCCGGGGGFEGHFH\n+@MISEQ:152:000000000-A8HE7:1:1101:19098:3666 1:N:0:ANATCG\n+ATCCACCCCTGCTTTCCGTTGTTTTAGAGC\n++\n+11AA@F1>DAAACDGFG11AEFGG0301A1\n+@MISEQ:152:000000000-A8HE7:1:1101:11814:3668 1:N:0:ANATCG\n+ACCCTCGTTCAGCGGCTTGAGTTTTAGAGC\n++\n+ABBABBBAAFF@F2EEG2EFEFGGHCFFFF\n+@MISEQ:152:000000000-A8HE7:1:1101:14609:3668 1:N:0:ANATCG\n+TTCTTACCTTGGGGGTAGTCCGTTATCGAT\n++\n+>3>A?5@CB5D44AAA2CFEFEGHFA5222\n+@MISEQ:152:000000000-A8HE7:1:1101:11790:3668 1:N:0:ANATCG\n+CTGCTGAAGCACCCCAACATGTTTTAGAGC\n++\n+>11>>1D1DFB1CAF1E1FGFEGDFFHFFH\n+@MISEQ:152:000000000-A8HE7:1:1101:13710:3668 1:N:0:ANATCG\n+TTACCCTGCCCACACTCCTGTTTTAGAGCT\n++\n+>>A3>CF4AFA4AEEGC44GGGFHGHHHHH\n+@MISEQ:152:000000000-A8HE7:1:1101:19043:3669 1:N:0:ANACCG\n+CTTATAGTCAGCCTCAAAAGTTTTAGAGCT\n++\n+BBABAFF5FFF44BGGGGGGB5FFFFFHHH\n+@MISEQ:152:000000000-A8HE7:1:1101:13832:3671 1:N:0:ANATCG\n+AAGAAGATGATGCAGAGTCCGTTATCAACT\n++\n+>>A11B1113BDGGE1FFGGFGHGG3F3FD\n+@MISEQ:152:000000000-A8HE7:1:1101:18171:3671 1:N:0:ANATAG\n+CATACCAGCTGAGCCGTCCGGCTTTAGAGC\n++\n+B@BBBFFFBFFFAGGGFGGGEGEGHHGHFH\n+@MISEQ:152:000000000-A8HE7:1:1101:13736:3671 1:N:0:ANATCG\n+CCCGGTCGGATGCGGCCCCCGTTTTAGAGC\n++\n+1>1AA>A1@1A0C0EE0EGGGGHHGHHHGH\n+@MISEQ:152:000000000-A8HE7:1:1101:17567:3671 1:N:0:ANATCG\n+GTCCAACGGAAAGTGGCCACGTTTTAGAGC\n++\n+?AAABFFABAADF55FFGGCGFGBAGHHFG\n+@MISEQ:152:000000000-A8HE7:1:1101:14881:3671 1:N:0:ANATCG\n+TCCGCAGCCCTCCACACGTAGTTTTAGAGC\n++\n+AAAA111>>1B>11B1A0B0EFHHGGCFHF\n+@MISEQ:152:000000000-A8HE7:1:1101:16313:3672 1:N:0:ANATCA\n+ACTGACCCTTTGCCGCAGCAGTTTAGAGCT\n++\n+1>1>AF1C1>@11FEGCAEAGFGBGBFHD1\n+@MISEQ:152:000000000-A8HE7:1:1101:15652:3672 1:N:0:ANATCT\n+CCCGATGATCTCGATGCCCGTTTTAGAGCT\n++\n+>A>A?AAD3DFFGF13A1CAEGGFFGGHGH\n+@MISEQ:152:000000000-A8HE7:1:1101:18193:3673 1:N:0:ANATCG\n+TTCGGAGCTGAGGAACATGCGTTTTAGAGC\n++\n+AA?AA3A@FCBF2C4AEDGBEEGGGHHHHH\n+@MISEQ:152:000000000-A8HE7:1:1101:17624:3673 1:N:0:ANATCG\n+CCATCATGCCACACAAGATCGTTTTAGAGC\n++\n+ABBBBF55DFFFGGCC4B4FCFGFFGFCFH\n+@MISEQ:152:000000000-A8HE7:1:1101:21520:3674 1:N:0:ANATCG\n+TTATCAACTTGAAAAAGTGGCACCGAGTCG\n++\n+AAAAABF1B@D1BEAFA11D111AEEC0B0\n+@MISEQ:152:000000000-A8HE7:1:1101:17841:3674 1:N:0:ANATAG\n+TCCATCTCGTTTGTTTTTCTGTTTTAGAGC\n++\n+BABBB5554AAAEGEGGGFBEGGHHGGBGG\n+@MISEQ:152:000000000-A8HE7:1:1101:21445:3674 1:N:0:ANATCG\n+TCTCCTATCAAGGCCATTCTGTTTTAGAGC\n++\n+B?ABBF45D55BEAGAFGFFGGHHHGH4GG\n+@MISEQ:152:000000000-A8HE7:1:1101:13569:3674 1:N:0:ANATCG\n+AGGATCCTGAGACGTTCCTCGTTTTAGAGC\n++\n+>AA1>CBFDF1D1E11BCFGGGGGGHHHHH\n+@MISEQ:152:000000000-A8HE7:1:1101:15838:3674 1:N:0:ANATCG\n+TTCCCTATCAGTAGAAATAGCAAGTCAAAA\n++\n+AA11111@B3BD333B31A11A11F13F3A\n+@MISEQ:152:000000000-A8HE7:1:1101:16429:3675 1:N:0:ANATCG\n+CTGGTCTCGCCGGTAGCTATGTTTTAGAGC\n++\n+AAA1AF3@1A111A001333GGHHGHHFHH\n+@MISEQ:152:000000000-A8HE7:1:1101:8184:3675 1:N:0:ANATCG\n+GACTCCTATCATCATGTCGGTTATCAACTT\n++\n+BABB@FFFFFFFGBGGDGGGEEDFFFGHHG\n+@MISEQ:152:000000000-A8HE7:1:1101:16716:3675 1:N:0:ANATCG\n+GCCCACATGCAACTTTGTTGGTTAGAGCTA\n++\n+>AA>1AAF11DFC113FB1BFAGH011FAF\n+@MISEQ:152:000000000-A8HE7:1:1101:10571:3675 1:N:0:ANATCG\n+TCTTCAATTGTGTAGCGAAGGTTTTAGGGC\n++\n+>1AAAFFFFF3BFGGGGGEECB11AFGGHG\n' |
b |
diff -r 000000000000 -r b8da4d41aa1d test-data/demo/demo2/test2.fastq --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/demo/demo2/test2.fastq Wed Feb 14 06:42:55 2018 -0500 |
b |
b'@@ -0,0 +1,10000 @@\n+@MISEQ:152:000000000-A8HE7:1:1101:12014:1936 1:N:0:GNCTAA\n+CACTACCTTAGAAATAGCACGTTAAAATAA\n++\n+>>11>FCFFFD1FDFGDFF1ABGHCF1AAB\n+@MISEQ:152:000000000-A8HE7:1:1101:13315:1937 1:N:0:GNCTAG\n+CCCATCGAGATCTCTCTGACGTTTTAGAGC\n++\n+1>11AFA11>>FFG31A3BB?FFHE3BA1A\n+@MISEQ:152:000000000-A8HE7:1:1101:17562:1953 1:N:0:GNCTAA\n+CACCAGCCACTTACTGTGCGTTTTAGAGCT\n++\n+AAAA11>A1CFFGGF3BA1AAEEE3011DF\n+@MISEQ:152:000000000-A8HE7:1:1101:14418:1961 1:N:0:GNCTAT\n+CGCTCAGCTGCCCAAACCCAGTTTTAGAGC\n++\n+AAA1A1>C11B1FFEGGF1FCGHHHGGFHG\n+@MISEQ:152:000000000-A8HE7:1:1101:19853:1969 1:N:0:GNCTAA\n+CCACGCAGCCGATCCAGTCCGTTTTAGAGC\n++\n+1AAA@ADD?A1>E0A1131BEGGFFFFFFG\n+@MISEQ:152:000000000-A8HE7:1:1101:13114:1978 1:N:0:GNCTAA\n+CGGGGGAGAACCCGACGCGTTTTAGAGCTA\n++\n+A@A?AD@?0CFAGG?EAEEAAEFGCGGHFH\n+@MISEQ:152:000000000-A8HE7:1:1101:19171:1980 1:N:0:GNCTAA\n+GAGTCCCTGAAGATGATCTTGTTTTAGAGC\n++\n+11>>AF1BFFFFFGGFFGGCGGHC13B1FF\n+@MISEQ:152:000000000-A8HE7:1:1101:13552:1987 1:N:0:GNCTAA\n+TCCTTATCATTTGCACCACCGTTTTAGAGC\n++\n+AAAAA3D1FBDBA3EDC11AEGGHGABAFF\n+@MISEQ:152:000000000-A8HE7:1:1101:14030:1991 1:N:0:GNCTAA\n+CCCAGTGCATCCTCGTCCCTGTTTTAGAGC\n++\n+AAAA1FFFF3DBBFAGFGGDFGGHGHHHHG\n+@MISEQ:152:000000000-A8HE7:1:1101:18336:1993 1:N:0:GNCTAA\n+CTAACATCACTTCTCTGTAGGTTTTAGAGC\n++\n+>>>>AF3B@D1BFGGGDAFGBGHG13ABFA\n+@MISEQ:152:000000000-A8HE7:1:1101:12130:2005 1:N:0:GNCTAA\n+GCCTGGTTTTAGAGCTAGAAATAGCAAGTT\n++\n+?AAAA?CDFAB>1F1FFF1B3G31BD1ABA\n+@MISEQ:152:000000000-A8HE7:1:1101:13467:2010 1:N:0:GNCTAA\n+CAACGTCCCCCACCTCCTCCGTTTTAGAGC\n++\n+A@AAAAAAABBBGGGGGFGGGGHHGGBG3F\n+@MISEQ:152:000000000-A8HE7:1:1101:14166:2013 1:N:0:GNCTAA\n+TCACCGTCCGCTGCGACTGGTTTTAGAGCT\n++\n+AA1AA1FAADDAGGGCEGGF1BEEFHHHGF\n+@MISEQ:152:000000000-A8HE7:1:1101:13401:2014 1:N:0:GNCTAA\n+TTTAGCAAGTTAAAATAAGGCTAGTCCGTT\n++\n+AAAA@DFF1BD33BGFGCGGGGFEE3D00F\n+@MISEQ:152:000000000-A8HE7:1:1101:13793:2029 1:N:0:GNCTAA\n+CTGCCAACATGATGAAGAAGGTTTTAGAGC\n++\n+A>AAAC@AA1CDGDEFGGGFGGHHGHHHHH\n+@MISEQ:152:000000000-A8HE7:1:1101:19424:2030 1:N:0:GNCTAA\n+TTACGTAGGACCTCTCAGCAGTTTTAGAGC\n++\n+1>AAACBF@1FAGFEGG3FFGGHGHGCEFF\n+@MISEQ:152:000000000-A8HE7:1:1101:13415:2031 1:N:0:GNCTAA\n+ACTCACCAAGACGCCCATCTGTTTTAGAGC\n++\n+1>AAAFFB1BFFEEG1E0FFGGHHGHGGGH\n+@MISEQ:152:000000000-A8HE7:1:1101:18009:2034 1:N:0:GNCTAA\n+ACTCTCTTACCTCTTCTCTAGTTTTAGAGC\n++\n+1AAA1B13@DFFGBFGGGGBFGHHFEGCFH\n+@MISEQ:152:000000000-A8HE7:1:1101:11975:2035 1:N:0:GNCTAA\n+ACCAGGCAGACAAACACTTGGTTTTAGAGC\n++\n+BBBBBB@BABF4GGGGGFGGGGHHEGGFGH\n+@MISEQ:152:000000000-A8HE7:1:1101:12052:2040 1:N:0:GNCTAA\n+CTTGATATCAATCCCACTCCGTTATCAACT\n++\n+1>A111BDFDFFFGBAG1F1EFGCHF33C3\n+@MISEQ:152:000000000-A8HE7:1:1101:17121:2041 1:N:0:GNCTAA\n+ACCTGCATTAGACTTGAATGGTTTTAGAGC\n++\n+1>A?A1B@D1@3G3BF1F3BGGHHCHB11A\n+@MISEQ:152:000000000-A8HE7:1:1101:14480:2041 1:N:0:GNCTAA\n+TTTCGCGATGCCTCAGTTCTGTTTTAGAGC\n++\n+AA1A?A?1A1AC3AC1FF3AEFGFHHHGHH\n+@MISEQ:152:000000000-A8HE7:1:1101:13535:2043 1:N:0:GNCTAA\n+CTTGATATCCATCCCACTCCGTTATCAACT\n++\n+AA>11BB3133@11A1B1BEGGHACD3FA3\n+@MISEQ:152:000000000-A8HE7:1:1101:12360:2045 1:N:0:GNCTAA\n+AACGCCAGCCCTCGAACCATGTTTTAGAGC\n++\n+AAAAADA?>AABGE0BA00AGGHHCGHHGG\n+@MISEQ:152:000000000-A8HE7:1:1101:18203:2050 1:N:0:GNCTAA\n+TCCTATACAGAAACTCTTTGGTTTTAGAGC\n++\n+A@AAA5DFF5FFCG5GFGFGCEGFDHHGHF\n+@MISEQ:152:000000000-A8HE7:1:1101:17008:2056 1:N:0:GNCTAA\n+TCGGACGCCTCACAAGGCTAGTCCGATATC\n++\n+1AAA>D?DD?AFAGF00F1FGF3D000A0D\n+@MISEQ:152:000000000-A8HE7:1:1101:16217:2059 1:N:0:GNCTAA\n+CCGACGTTCTCTTGCGGCATGTTTTAGAGC\n++\n+AAA>ADDAF@FFCGBE2EECEGFGHHGF3D\n+@MISEQ:152:000000000-A8HE7:1:1101:12782:2059 1:N:0:GNCTAA\n+TCCAAGGCCTCCCTCCACGTTTTAGAGCTA\n++\n+AAABAFAAACFBGGGGGGGGFGF5F4EGHC\n+@MISEQ:152:000000000-A8HE7:1:1101:13133:2059 1:N:0:GNCTAA\n+CCACGCCCCAACTGGAACAGTTTTAGAGCT\n++\n+AAAAAAADDAAABG1G11FBDGHHB01AB1\n+@MISEQ:152:000000000-A8HE7:1:1101:13454:2059 1:N:0:GNCTAA\n+CTGGGGAACTACCGGCTCAGTTTTAGAGCC\n++\n+B@?AADA@AFFFGCGEEEG4BGHHB433F2\n+@MISEQ:152:000000000-A8HE7:1:1101:12641:2062 1:N:0:GNCTAA\n+ATTGTGCATCTATAAGCTGCGTTTTAGAGC\n++\n+AAAAAFBFFFB@FFF3FF1FEGGGGHHHHH\n+@MISEQ:152:000000000-A8HE7:1:1101:17410:2064 1:N:0:GNCTAA\n+CTTACCCGTCGGA'..b'1101:16742:3186 1:N:0:GNCTAA\n+TTATATCTGCGTGATTTTCGGGTTTAGAGC\n++\n+ABBBBFFFFB3A??4BGGG?EE4AAFGGHG\n+@MISEQ:152:000000000-A8HE7:1:1101:12979:3187 1:N:0:GNCTAA\n+CTGGGTGGAGCGCTCTGTCCGTTTTAGAGC\n++\n+AABB3BA2AAFBGGGCFGGGGAFGFHGHHH\n+@MISEQ:152:000000000-A8HE7:1:1101:19431:3188 1:N:0:GNCTAA\n+GATAGCAGTTAACATAAGGCTAGTCCGTTA\n++\n+CC@BBFFFFFFFGFGGGGFGGGFF55?GFE\n+@MISEQ:152:000000000-A8HE7:1:1101:9502:3188 1:N:0:GNCTAA\n+TCGTCAACTGACTTGATGTGGTTTTAGAGC\n++\n+BBBBBBBFFFFFGGGGCGGGGBFGGGCGGF\n+@MISEQ:152:000000000-A8HE7:1:1101:11652:3189 1:N:0:GNCTAA\n+CAATCTACATCTTCTGCATTGTTTTAGAGC\n++\n+ABBBBFF5B5DFGGDBGDGFFGHHHGGHFF\n+@MISEQ:152:000000000-A8HE7:1:1101:16707:3189 1:N:0:ANCTAA\n+AGGCCAAGCGAGCAGTACAGGTTTTAGAGC\n++\n+AA?ABABBBBBBEECGEGGF4BBGFFFFGG\n+@MISEQ:152:000000000-A8HE7:1:1101:10583:3189 1:N:0:GNCCAA\n+ACAATGGCCAACCCTATTATGTTTTAGAGC\n++\n+AAA?AFBFFCBBECGFG5DFFGHHHHBGGH\n+@MISEQ:152:000000000-A8HE7:1:1101:19130:3189 1:N:0:GNCTAA\n+GTTTTACCGTTGCATCGTGGTTTAGAGCTA\n++\n+>>>AAFFFBAAA?GFGFAFEAFHHHAFGFG\n+@MISEQ:152:000000000-A8HE7:1:1101:18578:3189 1:N:0:GNCTAA\n+AGCTGTATGCACAAAGTTTGGTTTTAGAGC\n++\n+BABBBFFFBBFDGFGGGFFGCFHH2FCFFH\n+@MISEQ:152:000000000-A8HE7:1:1101:12107:3190 1:N:0:GNCTAA\n+TCACATTTATGTCACCCAGTGTTTTAGAGC\n++\n+BBBBBFFFFFFFGGGBG2AFGGHHC5AAGF\n+@MISEQ:152:000000000-A8HE7:1:1101:10057:3190 1:N:0:GNCTAA\n+GACCAGAACACGGGCTGGATGTTTTAGAGC\n++\n+B3AAAFFF4F42EEE?CE4DGBGGBGHFHG\n+@MISEQ:152:000000000-A8HE7:1:1101:19721:3190 1:N:0:GNCTAA\n+TGACAGGGAGGAGGAAACTTGTTTTAGAGC\n++\n+AAABBFAAAB@B2EEGGGGFGGHFHHHHHH\n+@MISEQ:152:000000000-A8HE7:1:1101:17999:3190 1:N:0:GNCTAA\n+TGTCCTGCAGTTAACTCAGTTTTAGAGCTA\n++\n+333AAFFFFBFBGDGGGGGGGGHHB4BGHF\n+@MISEQ:152:000000000-A8HE7:1:1101:9109:3191 1:N:0:GNCTAA\n+CTCAAGATCCCATCTGAGAGGTTTTAGAGC\n++\n+A3AA34DBFDFFFGGCBGGFAFFBFGF4GH\n+@MISEQ:152:000000000-A8HE7:1:1101:13192:3191 1:N:0:GNCTAA\n+TAGCACTTGCTGTAGACCCGTTTTAGAGCT\n++\n+CBCCCFFFFFFFGGFGGGGGGGHGGHHHHG\n+@MISEQ:152:000000000-A8HE7:1:1101:15114:3191 1:N:0:GNCTAA\n+GCTGTGACACTTGAACTTGTGTTTTAGAGC\n++\n+>AA?3BD5DCFFGFGFGGF5FGHFHGFFGG\n+@MISEQ:152:000000000-A8HE7:1:1101:18874:3191 1:N:0:GNCTAA\n+TCTGAAACATTATAGTGTCCGTTATCAACT\n++\n+1AA11@1DBDFFG3AG3GGG1AFFHGFHFE\n+@MISEQ:152:000000000-A8HE7:1:1101:16968:3191 1:N:0:GNCTAA\n+GATGGAGAAGACAGCGTACTGTTTTAGAGC\n++\n+AA@?AFFFFF11BA1EAE00BBFGHHHGGG\n+@MISEQ:152:000000000-A8HE7:1:1101:15434:3192 1:N:0:GNCCAA\n+TGTGGTTCCGGTCCGTCTTGGTTTTAGAGC\n++\n+1A1AA1>BB1AD?EE00FAFGFGHFHHBGH\n+@MISEQ:152:000000000-A8HE7:1:1101:17671:3192 1:N:0:GNATAA\n+TCAGCTCCTGCAGTTTCTTGGTTTTAGAGC\n++\n+A1AA111B1@CBABF33BFDFEA1A3B1B1\n+@MISEQ:152:000000000-A8HE7:1:1101:17035:3193 1:N:0:GNCTAA\n+CCTTTACCTAGAAGATCCATGTTTTAGAGC\n++\n+AAAAAFFFDFFFGGGGGBGCGGHHHGHGHH\n+@MISEQ:152:000000000-A8HE7:1:1101:18144:3193 1:N:0:GNCTAA\n+CCCGTTGTACCAGCCACGAGTTAGAGCTAT\n++\n+>AA>1CAAF3DB11A1F1AEEFHBDEA113\n+@MISEQ:152:000000000-A8HE7:1:1101:12031:3193 1:N:0:GNCTAA\n+CTCACAGCATTCCCGTTAGCGTTTTAGAGC\n++\n+BABBAFDAFFFFGGGGA2FGGGFGFCGGHF\n+@MISEQ:152:000000000-A8HE7:1:1101:19648:3194 1:N:0:GNCTAA\n+CCTGCACCGCGTGCGAGAGCGTTTTAGAGC\n++\n+AAAA@CFFD?D1EE0A0EE00AEEFFFGHH\n+@MISEQ:152:000000000-A8HE7:1:1101:18823:3194 1:N:0:GNCTAA\n+CCTGTTGCCCGCCGCCTGCAGTTTTAGAGC\n++\n+AAA?A11F1B>@EEAA0FGGFGHHDF0CGH\n+@MISEQ:152:000000000-A8HE7:1:1101:10505:3195 1:N:0:GNCTAA\n+TCAACCGCATCAGCCGTATCGTTTTAGAGC\n++\n+BB@BBFBDBBBFGGFGGGGFFGGFDFF3EB\n+@MISEQ:152:000000000-A8HE7:1:1101:16489:3195 1:N:0:GNCTAA\n+TCTTCGATTCTCAAAGTGTTTTAGAGCTAG\n++\n+ABABBBBFFFFFGBEG5BAAGGHHHDGGBE\n+@MISEQ:152:000000000-A8HE7:1:1101:19940:3195 1:N:0:GNATAA\n+ACGTCTGTCTGTACCAGCATGTTTTAGAGC\n++\n+BBBAAFBFFBFFGGBGGGGBGGHHB54445\n+@MISEQ:152:000000000-A8HE7:1:1101:20955:3195 1:N:0:GNCTAA\n+GTCCTTTGAAGAATTCTTTCGTTTTAGAGC\n++\n+A1>>A3@FD1111131A33GCBAAAFBBGF\n+@MISEQ:152:000000000-A8HE7:1:1101:9576:3196 1:N:0:GNCTAA\n+ACCTTTGAATCTCCCCAGCTGGTTTTAGAG\n++\n+BBBBBFBFFFFFDGFFG?FFFBBFGAGF44\n+@MISEQ:152:000000000-A8HE7:1:1101:11146:3196 1:N:0:GNCTAA\n+CGTCCTGTAGTTGTCCCCCCGTTTTAGAGC\n++\n+AA3>AAFFDDFFGFGGCGEG?AAABDG3AG\n+@MISEQ:152:000000000-A8HE7:1:1101:10918:3197 1:N:0:GNCTAA\n+ACCATTGGTTTACCATAAAAGTTTTAGAGC\n++\n+A1AAA11CDAFFFGG31F1FDFGGHB0FDF\n' |
b |
diff -r 000000000000 -r b8da4d41aa1d test-data/in.mageckQC.gmt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/in.mageckQC.gmt Wed Feb 14 06:42:55 2018 -0500 |
b |
@@ -0,0 +1,1 @@ +KEGG_RIBOSOME http://www.broadinstitute.org/gsea/msigdb/cards/KEGG_RIBOSOME RPL35 RPL23 RPL3 RPL3L RPL31 RPL4 RPL5 RPL32 RPS10 RPL34 RPS11 RPS12 RPL36AL RPS13 RPL35A RPL37A RPS15 RPL37 RPL38 RPL14 RPS15A FAU RPSA RPS16 RPL27 RPS17 RPL26 RPL24 RPL29 RPL28 RPL27A UBA52 RPL30 RPL36 RPS20 RPS19 RPS18 RPS4X RSL24D1P11 RPS4Y1 RPS5 RPL22L1 RPL26L1 RPS6 RPLP2 RPL22 RPL23A RPL18 RPL18A RPL19 RPL21 RPS3A RPL10A RPS27L RPS3 RPS2 MRPL13 RPL17 RPL15 RPL36A RPL12 RPL13 RPL41 RPL10 RPL39 RPL11 RPL8 RPL9 RPL13A RPL7A RPS26 RPS27 RPS25 RPS29 RPLP1 RPS27A RPLP0 RPS28 RPS24 RPS9 RSL24D1 RPS23 RPS8 RPS21 RPS7 RPL10L RPL7 RPL6 |
b |
diff -r 000000000000 -r b8da4d41aa1d test-data/in.mle.design_matrix.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/in.mle.design_matrix.txt Wed Feb 14 06:42:55 2018 -0500 |
b |
@@ -0,0 +1,5 @@ +Samples baseline HL60 KBM7 +HL60.initial 1 0 0 +KBM7.initial 1 0 0 +HL60.final 1 1 0 +KBM7.final 1 0 1 \ No newline at end of file |
b |
diff -r 000000000000 -r b8da4d41aa1d test-data/out.count.bam.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/out.count.bam.txt Wed Feb 14 06:42:55 2018 -0500 |
b |
b'@@ -0,0 +1,2551 @@\n+sgRNA\tGene\tsample1\n+s_10007\tCCNA1\t0\n+s_10008\tCCNA1\t0\n+s_10027\tCCNC\t0\n+s_10035\tCCND2\t0\n+s_10164\tCCS\t0\n+s_10200\tCCT7\t0\n+s_10232\tCD164\t0\n+s_10264\tCD1D\t0\n+s_10340\tCD276\t0\n+s_1035\tADAMTS14\t0\n+s_10381\tCD300LG\t0\n+s_10387\tCD320\t0\n+s_10401\tCD37\t0\n+s_10402\tCD37\t0\n+s_10405\tCD38\t0\n+s_10408\tCD3D\t0\n+s_10417\tCD3G\t0\n+s_10425\tCD40\t0\n+s_10434\tCD44\t0\n+s_10437\tCD46\t0\n+s_10448\tCD5\t0\n+s_10461\tCD55\t0\n+s_10481\tCD6\t0\n+s_10518\tCD81\t0\n+s_10541\tCD8B\t0\n+s_10563\tCD99L2\t0\n+s_10582\tCDC14B\t0\n+s_10594\tCDC20B\t0\n+s_10615\tCDC27\t0\n+s_10616\tCDC34\t0\n+s_1064\tADAMTS4\t0\n+s_10657\tCDC42SE2\t0\n+s_10658\tCDC42SE2\t0\n+s_10660\tCDC42SE2\t0\n+s_10675\tCDC73\t0\n+s_10731\tCDH13\t0\n+s_10739\tCDH16\t0\n+s_10770\tCDH23\t0\n+s_10785\tCDH3\t0\n+s_1085\tADAMTS9\t0\n+s_10862\tCDK17\t0\n+s_10943\tCDKL5\t0\n+s_10946\tCDKL5\t0\n+s_1101\tADAMTSL4\t0\n+s_11025\tCDSN\t0\n+s_11035\tCDX1\t0\n+s_11054\tCEACAM1\t0\n+s_11055\tCEACAM1\t0\n+s_11095\tCEBPB\t0\n+s_1113\tADAR\t0\n+s_11153\tCELF4\t0\n+s_1116\tADARB1\t0\n+s_11211\tCENPK\t0\n+s_11218\tCENPL\t0\n+s_11222\tCENPM\t0\n+s_1123\tADAT1\t0\n+s_11251\tCEP104\t0\n+s_1127\tADAT2\t0\n+s_11294\tCEP41\t0\n+s_11296\tCEP41\t0\n+s_11317\tCEP63\t0\n+s_1136\tADC\t0\n+s_11386\tCERS2\t0\n+s_11438\tCETN3\t0\n+s_11456\tCFH\t0\n+s_11513\tCGREF1\t0\n+s_11527\tCHAC1\t0\n+s_11585\tCHD1\t0\n+s_11598\tCHD3\t0\n+s_1161\tADCY10\t0\n+s_11692\tCHL1\t0\n+s_11714\tCHMP3\t0\n+s_11744\tCHN1\t0\n+s_1177\tADCY3\t0\n+s_11788\tCHRDL1\t0\n+s_1179\tADCY4\t0\n+s_118\tABCA12\t0\n+s_1183\tADCY5\t0\n+s_11837\tCHRNA9\t0\n+s_11862\tCHRNG\t0\n+s_11877\tCHST13\t0\n+s_11928\tCHUK\t0\n+s_11984\tCINP\t0\n+s_11988\tCIR1\t0\n+s_12015\tCITED1\t0\n+s_12019\tCIZ1\t0\n+s_12021\tCIZ1\t0\n+s_12049\tCKLF-CMTM1\t0\n+s_12057\tCKMT2\t0\n+s_12069\tCLCA1\t0\n+s_12082\tCLCC1\t0\n+s_12089\tCLCF1\t0\n+s_1212\tADD1\t0\n+s_12120\tCLCN7\t0\n+s_12121\tCLCN7\t0\n+s_12138\tCLDN10\t0\n+s_12140\tCLDN10\t0\n+s_12160\tCLDN17\t0\n+s_12177\tCLDN2\t0\n+s_12187\tCLDN25\t0\n+s_12197\tCLDN7\t0\n+s_12203\tCLDND1\t0\n+s_12209\tCLDND2\t0\n+s_1224\tADD2\t0\n+s_12262\tCLEC3B\t0\n+s_12310\tCLIC1\t0\n+s_1238\tADH4\t0\n+s_12420\tCLPTM1\t0\n+s_12425\tCLPX\t0\n+s_12433\tCLRN2\t0\n+s_12463\tCLTB\t0\n+s_12481\tCLUL1\t0\n+s_12522\tCMPK2\t0\n+s_12532\tCMTM1\t0\n+s_12576\tCNBP\t0\n+s_12578\tCNBP\t0\n+s_12589\tCNEP1R1\t0\n+s_12611\tCNGB1\t0\n+s_12624\tCNIH2\t0\n+s_12662\tCNNM3\t0\n+s_12666\tCNNM3\t0\n+s_12691\tCNOT4\t0\n+s_12695\tCNOT6\t0\n+s_12701\tCNOT7\t0\n+s_12703\tCNOT7\t0\n+s_12708\tCNP\t0\n+s_12738\tCNRIP1\t0\n+s_12764\tCNTN2\t0\n+s_12812\tCOASY\t0\n+s_12850\tCOG6\t0\n+s_12856\tCOG8\t0\n+s_12875\tCOL14A1\t0\n+s_12886\tCOL17A1\t0\n+s_12906\tCOL22A1\t0\n+s_12923\tCOL27A1\t0\n+s_1296\tADORA1\t0\n+s_12983\tCOL6A3\t0\n+s_12987\tCOL6A6\t0\n+s_13026\tCOLEC12\t0\n+s_13035\tCOMMD1\t0\n+s_13046\tCOMMD4\t0\n+s_13067\tCOMT\t0\n+s_13073\tCOPA\t0\n+s_13091\tCOPG1\t0\n+s_13123\tCOQ2\t0\n+s_13167\tCORO2A\t0\n+s_13190\tCOTL1\t0\n+s_13197\tCOX11\t0\n+s_13198\tCOX11\t0\n+s_13220\tCOX4I2\t0\n+s_13242\tCOX6C\t0\n+s_13244\tCOX6C\t0\n+s_13253\tCOX7A2L\t0\n+s_13287\tCPA5\t0\n+s_13290\tCPA6\t0\n+s_13309\tCPE\t0\n+s_13322\tCPEB3\t0\n+s_13328\tCPED1\t0\n+s_13338\tCPLX3\t0\n+s_13387\tCPO\t0\n+s_13389\tCPO\t0\n+s_13393\tCPOX\t0\n+s_13401\tCPPED1\t0\n+s_13405\tCPS1\t0\n+s_13427\tCPSF3L\t0\n+s_13428\tCPSF3L\t0\n+s_13486\tCR2\t0\n+s_1351\tADRA2A\t0\n+s_13512\tCRB1\t0\n+s_13554\tCREB3L3\t0\n+s_13557\tCREB3L4\t0\n+s_1357\tADRB1\t0\n+s_13572\tCREBL2\t0\n+s_13575\tCREBRF\t0\n+s_13579\tCREBZF\t0\n+s_13595\tCREM\t0\n+s_13602\tCRH\t0\n+s_1366\tADRBK2\t0\n+s_13662\tCRK\t0\n+s_13683\tCRMP1\t0\n+s_13686\tCRMP1\t0\n+s_13702\tCROT\t0\n+s_13718\tCRTAM\t0\n+s_13730\tCRTC2\t0\n+s_13755\tCRYBA1\t0\n+s_1376\tADSS\t0\n+s_13856\tCSF3\t0\n+s_13857\tCSF3\t0\n+s_13863\tCSF3R\t0\n+s_13874\tCSH2\t0\n+s_13879\tCSHL1\t0\n+s_1393\tAEN\t0\n+s_13930\tCSNK1G3\t0\n+s_13937\tCSNK2A1\t0\n+s_1394\tAEN\t0\n+s_13958\tCSPP1\t0\n+s_1396\tAES\t0\n+s_13985\tCSRP3\t0\n+s_13990\tCST11\t0\n+s_14021\tCSTA\t0\n+s_14052\tCT62\t0\n+s_14099\tCTCFL\t0\n+s_14107\tCTD-2616J11.4\t0\n+s_14153\tCTIF\t0\n+s_14157\tCTLA4\t0\n+s_14161\tCTLA4\t0\n+s_14163\tCTNNA1\t0\n+s_14187\tCTNND2\t0\n+s_14189\tCTNND2\t0\n+s_14209\tCTRL\t0\n+s_14226\tCTSC\t0\n+s_14264\tCTSS\t0\n+s_14266\tCTSW\t0\n+s_14274\tCTTN\t0\n+s_14282\tCTU1\t0\n+s_14286\tCTU2\t0\n+s_14338\tCUTA\t0\n+s_14364\tCWF19L1\t0\n+s_14369\tCWH43\t0\n+s_14370\tCWH43\t0\n+s_14418\tCXCL3\t0\n+s_14425\tCXCL6\t0\n+s_14439\tCXCR4\t0\n+s_14454\tCXXC1\t0\n+s_14463\tCXXC4\t0\n+s_14467\tCXXC5\t0\n+s_14485\tCXorf27\t0\n+s_14497\tCXorf40A\t0\n+s_1450\tAGA\t0\n+s_14520\tCXorf61\t0\n+s_14555\tCYB5B\t0\n+s_14576\tCYB5R4\t0\n+s_14586\tCYBA\t0\n+s_14670\tC'..b'\tZFC3H1\t0\n+s_62985\tZFHX3\t0\n+s_63034\tZFP64\t0\n+s_63079\tZFYVE1\t0\n+s_63097\tZFYVE21\t0\n+s_63104\tZFYVE27\t0\n+s_63107\tZFYVE27\t0\n+s_63114\tZFYVE28\t0\n+s_63149\tZIC2\t0\n+s_63192\tZMAT1\t0\n+s_63195\tZMAT2\t0\n+s_63208\tZMAT5\t0\n+s_63217\tZMIZ2\t0\n+s_63228\tZMYM3\t0\n+s_63232\tZMYM3\t0\n+s_63234\tZMYM3\t0\n+s_6326\tC12orf49\t0\n+s_63302\tZNF132\t0\n+s_63316\tZNF138\t0\n+s_63362\tZNF167\t0\n+s_63368\tZNF169\t0\n+s_6339\tC12orf53\t0\n+s_63396\tZNF182\t0\n+s_63398\tZNF184\t0\n+s_63435\tZNF200\t0\n+s_63487\tZNF223\t0\n+s_63489\tZNF224\t0\n+s_63517\tZNF233\t0\n+s_63594\tZNF276\t0\n+s_636\tACO1\t0\n+s_63600\tZNF28\t0\n+s_63612\tZNF280D\t0\n+s_63701\tZNF330\t0\n+s_63746\tZNF354B\t0\n+s_63755\tZNF362\t0\n+s_6376\tC12orf74\t0\n+s_63774\tZNF37A\t0\n+s_63785\tZNF384\t0\n+s_63798\tZNF385D\t0\n+s_63818\tZNF397\t0\n+s_63835\tZNF410\t0\n+s_63893\tZNF433\t0\n+s_63903\tZNF436\t0\n+s_63905\tZNF438\t0\n+s_63923\tZNF442\t0\n+s_63934\tZNF445\t0\n+s_63935\tZNF446\t0\n+s_63964\tZNF469\t0\n+s_63983\tZNF480\t0\n+s_64012\tZNF496\t0\n+s_64039\tZNF506\t0\n+s_6409\tC14orf105\t0\n+s_64098\tZNF534\t0\n+s_64137\tZNF554\t0\n+s_64147\tZNF558\t0\n+s_64149\tZNF558\t0\n+s_64166\tZNF563\t0\n+s_64241\tZNF586\t0\n+s_6427\tC14orf133\t0\n+s_64297\tZNF611\t0\n+s_64356\tZNF639\t0\n+s_6438\tC14orf159\t0\n+s_64380\tZNF646\t0\n+s_64393\tZNF655\t0\n+s_64395\tZNF655\t0\n+s_64396\tZNF655\t0\n+s_64419\tZNF668\t0\n+s_64424\tZNF669\t0\n+s_64432\tZNF671\t0\n+s_64459\tZNF682\t0\n+s_64465\tZNF684\t0\n+s_64479\tZNF688\t0\n+s_64491\tZNF692\t0\n+s_6450\tC14orf176\t0\n+s_64581\tZNF746\t0\n+s_64601\tZNF75D\t0\n+s_64627\tZNF772\t0\n+s_64628\tZNF772\t0\n+s_64633\tZNF774\t0\n+s_64637\tZNF775\t0\n+s_64638\tZNF776\t0\n+s_64652\tZNF780A\t0\n+s_64662\tZNF783\t0\n+s_64791\tZNF85\t0\n+s_64839\tZNHIT6\t0\n+s_64851\tZNRF3\t0\n+s_64862\tZP2\t0\n+s_64868\tZP4\t0\n+s_64869\tZPBP\t0\n+s_64871\tZPBP\t0\n+s_64878\tZPLD1\t0\n+s_64898\tZSCAN10\t0\n+s_64923\tZSCAN22\t0\n+s_64930\tZSCAN30\t0\n+s_64995\tZYG11A\t0\n+s_64997\tZYG11A\t0\n+s_6525\tC15orf39\t0\n+s_6592\tC16orf13\t0\n+s_6618\tC16orf53\t0\n+s_6639\tC16orf62\t0\n+s_6674\tC16orf86\t0\n+s_6707\tC17orf102\t0\n+s_6710\tC17orf104\t0\n+s_6728\tC17orf112\t0\n+s_6736\tC17orf39\t0\n+s_6794\tC17orf72\t0\n+s_6814\tC17orf80\t0\n+s_6827\tC17orf90\t0\n+s_6844\tC18orf1\t0\n+s_6849\tC18orf21\t0\n+s_6859\tC18orf32\t0\n+s_6862\tC18orf34\t0\n+s_6867\tC18orf54\t0\n+s_6906\tC19orf38\t0\n+s_691\tACP1\t0\n+s_6922\tC19orf44\t0\n+s_7045\tC1QTNF6\t0\n+s_7053\tC1QTNF7\t0\n+s_7059\tC1QTNF9B\t0\n+s_707\tACPL2\t0\n+s_7088\tC1orf106\t0\n+s_7105\tC1orf111\t0\n+s_7128\tC1orf122\t0\n+s_714\tACPT\t0\n+s_7144\tC1orf130\t0\n+s_7162\tC1orf144\t0\n+s_7217\tC1orf186\t0\n+s_7234\tC1orf198\t0\n+s_7252\tC1orf212\t0\n+s_730\tACSBG1\t0\n+s_7306\tC1orf49\t0\n+s_7336\tC1orf61\t0\n+s_7341\tC1orf63\t0\n+s_7465\tC20orf26\t0\n+s_747\tACSL1\t0\n+s_7498\tC20orf96\t0\n+s_7539\tC22orf25\t0\n+s_7562\tC22orf42\t0\n+s_76\tAARS2\t0\n+s_7626\tC2orf43\t0\n+s_7629\tC2orf44\t0\n+s_7674\tC2orf57\t0\n+s_7681\tC2orf62\t0\n+s_7692\tC2orf63\t0\n+s_77\tAARSD1\t0\n+s_7715\tC2orf70\t0\n+s_7793\tC3orf18\t0\n+s_7794\tC3orf18\t0\n+s_78\tAARSD1\t0\n+s_7803\tC3orf22\t0\n+s_781\tACSS1\t0\n+s_782\tACSS1\t0\n+s_7822\tC3orf27\t0\n+s_7838\tC3orf35\t0\n+s_786\tACSS2\t0\n+s_791\tACSS3\t0\n+s_7927\tC4orf19\t0\n+s_7940\tC4orf26\t0\n+s_7970\tC4orf37\t0\n+s_8000\tC4orf52\t0\n+s_804\tACTB\t0\n+s_8073\tC5orf51\t0\n+s_8141\tC6orf162\t0\n+s_8148\tC6orf165\t0\n+s_82\tAARSD1\t0\n+s_8227\tC7orf10\t0\n+s_825\tACTL6A\t0\n+s_8281\tC7orf59\t0\n+s_8318\tC8A\t0\n+s_8403\tC9orf100\t0\n+s_8419\tC9orf123\t0\n+s_8470\tC9orf24\t0\n+s_8476\tC9orf3\t0\n+s_8498\tC9orf50\t0\n+s_8514\tC9orf71\t0\n+s_8592\tCA6\t0\n+s_8699\tCACNA1G\t0\n+s_8705\tCACNA1I\t0\n+s_871\tACTR8\t0\n+s_8738\tCACNB4\t0\n+s_874\tACTR8\t0\n+s_8757\tCACNG5\t0\n+s_8795\tCADPS\t0\n+s_8797\tCADPS\t0\n+s_8848\tCALHM2\t0\n+s_8879\tCALR\t0\n+s_8880\tCALR\t0\n+s_8907\tCAMK2A\t0\n+s_8910\tCAMK2B\t0\n+s_8917\tCAMK2G\t0\n+s_8918\tCAMK2N1\t0\n+s_893\tACVR1B\t0\n+s_8930\tCAMKK1\t0\n+s_8939\tCAMKV\t0\n+s_8954\tCAMSAP1\t0\n+s_9038\tCAPN3\t0\n+s_906\tACVR2A\t0\n+s_9064\tCAPRIN1\t0\n+s_9077\tCAPSL\t0\n+s_9109\tCARD17\t0\n+s_9124\tCARD8\t0\n+s_9126\tCARD8\t0\n+s_913\tACY1\t0\n+s_9161\tCASC1\t0\n+s_9171\tCASD1\t0\n+s_9196\tCASP10\t0\n+s_9221\tCASP4\t0\n+s_9223\tCASP5\t0\n+s_9257\tCASR\t0\n+s_9285\tCATSPER3\t0\n+s_9506\tCCDC120\t0\n+s_9507\tCCDC121\t0\n+s_9517\tCCDC125\t0\n+s_952\tADAM12\t0\n+s_9584\tCCDC149\t0\n+s_964\tADAM18\t0\n+s_9646\tCCDC170\t0\n+s_9678\tCCDC28B\t0\n+s_9710\tCCDC40\t0\n+s_9725\tCCDC43\t0\n+s_9732\tCCDC48\t0\n+s_976\tADAM21\t0\n+s_9763\tCCDC62\t0\n+s_9791\tCCDC69\t0\n+s_9868\tCCDC89\t0\n+s_991\tADAM30\t0\n+s_9925\tCCL1\t0\n+s_9973\tCCL26\t0\n' |
b |
diff -r 000000000000 -r b8da4d41aa1d test-data/out.count.fastq.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/out.count.fastq.txt Wed Feb 14 06:42:55 2018 -0500 |
b |
b'@@ -0,0 +1,2551 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PD52\t0\n+s_58533\tTPI1\t0\n+s_5857\tBSPRY\t0\n+s_58612\tTPSG1\t0\n+s_58633\tTRA2B\t0\n+s_58655\tTRAF3\t0\n+s_58668\tTRAF3IP2\t0\n+s_58690\tTRAK1\t0\n+s_58809\tTRIB2\t0\n+s_58962\tTRIM50\t0\n+s_58968\tTRIM52\t0\n+s_59050\tTRIO\t0\n+s_59107\tTRMT1L\t0\n+s_59133\tTRMT61B\t0\n+s_59160\tTROVE2\t0\n+s_59173\tTRPC4\t0\n+s_59196\tTRPM1\t0\n+s_59204\tTRPM3\t0\n+s_59311\tTSEN54\t0\n+s_59332\tTSHB\t0\n+s_59340\tTSHZ2\t0\n+s_59360\tTSNARE1\t0\n+s_5952\tBTG4\t0\n+s_59539\tTTC21A\t0\n+s_59602\tTTC39A\t0\n+s_59654\tTTC9C\t0\n+s_59717\tTTLL6\t0\n+s_5974\tBTN3A1\t0\n+s_59748\tTTYH1\t0\n+s_59807\tTUBB2B\t0\n+s_59859\tTULP1\t0\n+s_59870\tTULP3\t0\n+s_59955\tTXNDC8\t0\n+s_59983\tTXNRD2\t0\n+s_600\tACE\t0\n+s_60169\tUBE2H\t0\n+s_60209\tUBE2Q2\t0\n+s_60237\tUBE2V2\t0\n+s_60248\tUBE3A\t0\n+s_60250\tUBE3B\t0\n+s_60373\tUBXN6\t0\n+s_60396\tUCKL1\t0\n+s_60423\tUEVLD\t0\n+s_60438\tUFSP1\t0\n+s_60449\tUGDH\t0\n+s_60517\tUGT2A1\t0\n+s_60542\tUGT3A1\t0\n+s_60603\tUMODL1\t0\n+s_60614\tUNC119\t0\n+s_60649\tUNC5B\t0\n+s_6068\tC10orf125\t0\n+s_6071\tC10orf128\t0\n+s_60753\tUQCRC2\t0\n+s_60780\tURM1\t0\n+s_60839\tUSP15\t0\n+s_60851\tUSP19\t0\n+s_60925\tUSP4\t0\n+s_6100\tC10orf53\t0\n+s_6106\tC10orf54\t0\n+s_61149\tVAV2\t0\n+s_61173\tVCAM1\t0\n+s_61178\tVCAN\t0\n+s_61221\tVEPH1\t0\n+s_61263\tVIL1\t0\n+s_61341\tVPS13C\t0\n+s_61344\tVPS13D\t0\n+s_61367\tVPS29\t0\n+s_61529\tVWA5A\t0\n+s_61531\tVWA5A\t0\n+s_61587\tWBP1\t0\n+s_61595\tWBP2\t0\n+s_61623\tWDFY1\t0\n+s_61640\tWDHD1\t0\n+s_61662\tWDR16\t0\n+s_61695\tWDR26\t0\n+s_61739\tWDR44\t0\n+s_6200\tC11orf49\t0\n+s_62019\tWISP1\t0\n+s_62098\tWNT5B\t0\n+s_62114\tWNT8A\t0\n+s_62171\tWTAP\t0\n+s_62249\tXKR3\t0\n+s_62257\tXKR6\t0\n+s_62275\tXPC\t0\n+s_62320\tXRCC4\t0\n+s_62361\tYAE1D1\t0\n+s_62550\tZBBX\t0\n+s_62559\tZBED6\t0\n+s_62567\tZBTB1\t0\n+s_62624\tZBTB37\t0\n+s_62657\tZBTB47\t0\n+s_62759\tZC3H7A\t0\n+s_62845\tZDHHC11\t0\n+s_62862\tZDHHC16\t0\n+s_62881\tZDHHC2\t0\n+s_6292\tC12orf23\t0\n+s_62975\tZFC3H1\t0\n+s_63034\tZFP64\t0\n+s_63104\tZFYVE27\t0\n+s_63107\tZFYVE27\t0\n+s_63114\tZFYVE28\t0\n+s_63217\tZMIZ2\t0\n+s_63228\tZMYM3\t0\n+s_63234\tZMYM3\t0\n+s_6326\tC12orf49\t0\n+s_63302\tZNF132\t0\n+s_63362\tZNF167\t0\n+s_63435\tZNF200\t0\n+s_63487\tZNF223\t0\n+s_63594\tZNF276\t0\n+s_636\tACO1\t0\n+s_63746\tZNF354B\t0\n+s_63755\tZNF362\t0\n+s_6376\tC12orf74\t0\n+s_63903\tZNF436\t0\n+s_63905\tZNF438\t0\n+s_63923\tZNF442\t0\n+s_63934\tZNF445\t0\n+s_63935\tZNF446\t0\n+s_63964\tZNF469\t0\n+s_63983\tZNF480\t0\n+s_6409\tC14orf105\t0\n+s_64137\tZNF554\t0\n+s_64241\tZNF586\t0\n+s_6427\tC14orf133\t0\n+s_64356\tZNF639\t0\n+s_64393\tZNF655\t0\n+s_64396\tZNF655\t0\n+s_64419\tZNF668\t0\n+s_64424\tZNF669\t0\n+s_64459\tZNF682\t0\n+s_64479\tZNF688\t0\n+s_64581\tZNF746\t0\n+s_64627\tZNF772\t0\n+s_64638\tZNF776\t0\n+s_64652\tZNF780A\t0\n+s_64791\tZNF85\t0\n+s_64851\tZNRF3\t0\n+s_64871\tZPBP\t0\n+s_64878\tZPLD1\t0\n+s_64898\tZSCAN10\t0\n+s_64930\tZSCAN30\t0\n+s_64997\tZYG11A\t0\n+s_6525\tC15orf39\t0\n+s_6592\tC16orf13\t0\n+s_6639\tC16orf62\t0\n+s_6707\tC17orf102\t0\n+s_6710\tC17orf104\t0\n+s_6728\tC17orf112\t0\n+s_6736\tC17orf39\t0\n+s_6794\tC17orf72\t0\n+s_6814\tC17orf80\t0\n+s_6849\tC18orf21\t0\n+s_6859\tC18orf32\t0\n+s_6862\tC18orf34\t0\n+s_6906\tC19orf38\t0\n+s_7053\tC1QTNF7\t0\n+s_7128\tC1orf122\t0\n+s_7144\tC1orf130\t0\n+s_7162\tC1orf144\t0\n+s_7234\tC1orf198\t0\n+s_7341\tC1orf63\t0\n+s_747\tACSL1\t0\n+s_76\tAARS2\t0\n+s_7674\tC2orf57\t0\n+s_7681\tC2orf62\t0\n+s_7692\tC2orf63\t0\n+s_77\tAARSD1\t0\n+s_78\tAARSD1\t0\n+s_781\tACSS1\t0\n+s_786\tACSS2\t0\n+s_7940\tC4orf26\t0\n+s_7970\tC4orf37\t0\n+s_8000\tC4orf52\t0\n+s_804\tACTB\t0\n+s_8073\tC5orf51\t0\n+s_8141\tC6orf162\t0\n+s_8227\tC7orf10\t0\n+s_8281\tC7orf59\t0\n+s_8318\tC8A\t0\n+s_8403\tC9orf100\t0\n+s_8470\tC9orf24\t0\n+s_8699\tCACNA1G\t0\n+s_8705\tCACNA1I\t0\n+s_871\tACTR8\t0\n+s_874\tACTR8\t0\n+s_8757\tCACNG5\t0\n+s_8797\tCADPS\t0\n+s_8879\tCALR\t0\n+s_8910\tCAMK2B\t0\n+s_893\tACVR1B\t0\n+s_8930\tCAMKK1\t0\n+s_8954\tCAMSAP1\t0\n+s_9064\tCAPRIN1\t0\n+s_9077\tCAPSL\t0\n+s_9109\tCARD17\t0\n+s_913\tACY1\t0\n+s_9171\tCASD1\t0\n+s_9196\tCASP10\t0\n+s_9285\tCATSPER3\t0\n+s_9506\tCCDC120\t0\n+s_9507\tCCDC121\t0\n+s_952\tADAM12\t0\n+s_9584\tCCDC149\t0\n+s_964\tADAM18\t0\n+s_9646\tCCDC170\t0\n+s_9710\tCCDC40\t0\n+s_9732\tCCDC48\t0\n+s_976\tADAM21\t0\n+s_9763\tCCDC62\t0\n+s_9868\tCCDC89\t0\n+s_991\tADAM30\t0\n+s_9925\tCCL1\t0\n+s_9973\tCCL26\t0\n' |
b |
diff -r 000000000000 -r b8da4d41aa1d test-data/out.count.log.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/out.count.log.txt Wed Feb 14 06:42:55 2018 -0500 |
b |
@@ -0,0 +1,46 @@ +INFO @ Wed, 14 Feb 2018 01:52:58: Parameters: /home/maria/miniconda3/envs/mulled-v1-0142cfe25b04f0c1d6899e250fb2f311b2d84778259938a0f6bd1d2ee743fa71/bin/mageck count -l /tmp/tmpqZ84Xc/files/000/dataset_7.dat --fastq input.gz -n output --pdf-report --keep-tmp --unmapped-to-file --norm-method median --sgrna-len 20 +INFO @ Wed, 14 Feb 2018 01:52:58: Welcome to MAGeCK v0.5.7. Command: count +INFO @ Wed, 14 Feb 2018 01:52:58: Loading 2550 predefined sgRNAs. +WARNING @ Wed, 14 Feb 2018 01:52:58: There are 0 sgRNAs with duplicated sequences. +INFO @ Wed, 14 Feb 2018 01:52:58: Parsing FASTQ file input.gz... +INFO @ Wed, 14 Feb 2018 01:52:58: Determining the trim-5 length of FASTQ file input.gz... +INFO @ Wed, 14 Feb 2018 01:52:58: Possible gRNA lengths:20 +INFO @ Wed, 14 Feb 2018 01:52:58: Processing 0M reads ... +INFO @ Wed, 14 Feb 2018 01:52:58: Read length:30 +INFO @ Wed, 14 Feb 2018 01:52:58: Total tested reads: 2500, mapped: 1453(0.5812) +INFO @ Wed, 14 Feb 2018 01:52:58: --trim-5 test data: (trim_length reads fraction) +INFO @ Wed, 14 Feb 2018 01:52:58: 0 1453 1.0 +INFO @ Wed, 14 Feb 2018 01:52:58: Auto determination of trim5 results: 0 +INFO @ Wed, 14 Feb 2018 01:52:58: Possible gRNA lengths:20 +INFO @ Wed, 14 Feb 2018 01:52:58: Processing 0M reads .. +INFO @ Wed, 14 Feb 2018 01:52:58: Total: 2500. +INFO @ Wed, 14 Feb 2018 01:52:58: Mapped: 1453. +DEBUG @ Wed, 14 Feb 2018 01:52:58: Initial (total) size factor: 1.0 +DEBUG @ Wed, 14 Feb 2018 01:52:58: Median factor: 2.0 +INFO @ Wed, 14 Feb 2018 01:52:58: Final size factor: 2.0 +INFO @ Wed, 14 Feb 2018 01:52:58: Summary of file input.gz: +INFO @ Wed, 14 Feb 2018 01:52:58: label sample1 +INFO @ Wed, 14 Feb 2018 01:52:58: reads 2500 +INFO @ Wed, 14 Feb 2018 01:52:58: mappedreads 1453 +INFO @ Wed, 14 Feb 2018 01:52:58: totalsgrnas 2550 +INFO @ Wed, 14 Feb 2018 01:52:58: zerosgrnas 1276 +INFO @ Wed, 14 Feb 2018 01:52:58: giniindex 0.5266899931488773 +INFO @ Wed, 14 Feb 2018 01:52:58: Loading Rnw template file: /home/maria/miniconda3/envs/mulled-v1-0142cfe25b04f0c1d6899e250fb2f311b2d84778259938a0f6bd1d2ee743fa71/lib/python3.6/site-packages/mageck/fastq_template.Rnw. +DEBUG @ Wed, 14 Feb 2018 01:52:58: Setting up the visualization module... +INFO @ Wed, 14 Feb 2018 01:52:58: Running command: cd ./; Rscript output_countsummary.R +INFO @ Wed, 14 Feb 2018 01:52:58: Command message: +INFO @ Wed, 14 Feb 2018 01:52:58: Writing to file output_countsummary.tex +INFO @ Wed, 14 Feb 2018 01:52:58: Processing code chunks with options ... +INFO @ Wed, 14 Feb 2018 01:52:58: 1 : keep.source term verbatim (label = funcdef, output_countsummary.Rnw:28) +INFO @ Wed, 14 Feb 2018 01:52:58: 2 : keep.source term tex (label = tab1, output_countsummary.Rnw:156) +INFO @ Wed, 14 Feb 2018 01:52:58: 3 : keep.source term tex (label = tab2, output_countsummary.Rnw:174) +INFO @ Wed, 14 Feb 2018 01:52:58: 4 : keep.source term tex (label = tab3, output_countsummary.Rnw:188) +INFO @ Wed, 14 Feb 2018 01:52:58: 5 : keep.source term verbatim pdf (output_countsummary.Rnw:221) +INFO @ Wed, 14 Feb 2018 01:52:58: 6 : keep.source term verbatim pdf (output_countsummary.Rnw:228) +INFO @ Wed, 14 Feb 2018 01:52:58: +INFO @ Wed, 14 Feb 2018 01:52:58: You can now run (pdf)latex on ‘output_countsummary.tex’ +INFO @ Wed, 14 Feb 2018 01:52:58: Error in texi2dvi("output_countsummary.tex", pdf = TRUE) : +INFO @ Wed, 14 Feb 2018 01:52:58: pdflatex is not available +INFO @ Wed, 14 Feb 2018 01:52:58: Execution halted +INFO @ Wed, 14 Feb 2018 01:52:58: +INFO @ Wed, 14 Feb 2018 01:52:58: End command message. |
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diff -r 000000000000 -r b8da4d41aa1d test-data/out.count.unmapped.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/out.count.unmapped.txt Wed Feb 14 06:42:55 2018 -0500 |
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b'@@ -0,0 +1,935 @@\n+CAGAAATACAGTGCGACCTG\tCAGAAATACAGTGCGACCTG\t1\n+CGATCCACAATTTTAAAAGA\tCGATCCACAATTTTAAAAGA\t1\n+ATCACATCTGTTTTAGAGCT\tATCACATCTGTTTTAGAGCT\t1\n+TGTTGCAAGGCTAGTCCGTT\tTGTTGCAAGGCTAGTCCGTT\t1\n+CCAGAACGACCACGTCTGTT\tCCAGAACGACCACGTCTGTT\t1\n+TACCTTGGATCACTTACATC\tTACCTTGGATCACTTACATC\t1\n+CTTCAAGCTGTAATTCCGTA\tCTTCAAGCTGTAATTCCGTA\t1\n+TGAAAGTACTAGAAAACGTG\tTGAAAGTACTAGAAAACGTG\t1\n+TCTCACCGTGCTGTCACATT\tTCTCACCGTGCTGTCACATT\t1\n+GGGCGATGCACCGTTAATCG\tGGGCGATGCACCGTTAATCG\t1\n+CAGCTACTTCTTCCTTGCGC\tCAGCTACTTCTTCCTTGCGC\t1\n+CCTCTGACCTCAAGTTAAAA\tCCTCTGACCTCAAGTTAAAA\t1\n+TCTCCAAAATAAGGCTAGTC\tTCTCCAAAATAAGGCTAGTC\t5\n+TCCGCCGGGCGATCCGCCTG\tTCCGCCGGGCGATCCGCCTG\t1\n+CCCATCTCTTGTCTCTGTTT\tCCCATCTCTTGTCTCTGTTT\t1\n+CCCACCACTTATTTTTCCAC\tCCCACCACTTATTTTTCCAC\t1\n+TAAGCACGTTCGAGTCCATG\tTAAGCACGTTCGAGTCCATG\t1\n+ATTAGAGCTAGAAATAGCAA\tATTAGAGCTAGAAATAGCAA\t1\n+TACGCTGACCTCCGCAACCT\tTACGCTGACCTCCGCAACCT\t1\n+GACTCCTATCATCATGTCGG\tGACTCCTATCATCATGTCGG\t4\n+CTACACAGATCCTTCCGTGA\tCTACACAGATCCTTCCGTGA\t1\n+ATCTTTCATGTCGAGACACA\tATCTTTCATGTCGAGACACA\t1\n+CTTCACGATGCCAGCCGTTT\tCTTCACGATGCCAGCCGTTT\t1\n+ACCCGCGTCCTCGTCGTCGA\tACCCGCGTCCTCGTCGTCGA\t1\n+TCCGCGCTTCCAGCCGCTCC\tTCCGCGCTTCCAGCCGCTCC\t1\n+TGTTCTTACCTGTGTTCGCG\tTGTTCTTACCTGTGTTCGCG\t1\n+TAGCCAGAAAACTTTACTCT\tTAGCCAGAAAACTTTACTCT\t1\n+CTCAAAATAGCGAGTTAAAA\tCTCAAAATAGCGAGTTAAAA\t5\n+CTCCAACCGTCCGTTATCAA\tCTCCAACCGTCCGTTATCAA\t2\n+CCGCGCACCACCGAGTCGGT\tCCGCGCACCACCGAGTCGGT\t3\n+TTTTGGGAGTACCGTGGACT\tTTTTGGGAGTACCGTGGACT\t1\n+AGTCCTGTCCCATGCGATTG\tAGTCCTGTCCCATGCGATTG\t1\n+CGCTGCCATCAACTTGAAAA\tCGCTGCCATCAACTTGAAAA\t6\n+CCTGCGTCCCGTGCAGGTGA\tCCTGCGTCCCGTGCAGGTGA\t1\n+GGGTAGTGACCCGTCCGTTA\tGGGTAGTGACCCGTCCGTTA\t2\n+CTTCAGTCCAAATGTAGCCG\tCTTCAGTCCAAATGTAGCCG\t1\n+CATGCCTCTTCATAGTTAAG\tCATGCCTCTTCATAGTTAAG\t1\n+TTCAGCAAGTTAAAATAAGG\tTTCAGCAAGTTAAAATAAGG\t6\n+CACGCTGACCCAGCCTATCG\tCACGCTGACCCAGCCTATCG\t1\n+TGCCGTCTTGGCGGGCCCCC\tTGCCGTCTTGGCGGGCCCCC\t1\n+CACGGTAACGGAGCGATTTG\tCACGGTAACGGAGCGATTTG\t1\n+TCCTCTCATGTGAAGTTATT\tTCCTCTCATGTGAAGTTATT\t1\n+CCAGCTTGTTCCTCTCCCCC\tCCAGCTTGTTCCTCTCCCCC\t1\n+GCTGTACCTGGAAAATCATG\tGCTGTACCTGGAAAATCATG\t1\n+TGCTTGGGCCGCTCCTCACA\tTGCTTGGGCCGCTCCTCACA\t1\n+GGGCAAGTCCGTTATCAACT\tGGGCAAGTCCGTTATCAACT\t4\n+GATGACACACCTGACCAGCC\tGATGACACACCTGACCAGCC\t1\n+TCTACATCTCCGACCACGTT\tTCTACATCTCCGACCACGTT\t1\n+CTGTCCGTTCTCAACTTGAA\tCTGTCCGTTCTCAACTTGAA\t4\n+GCAGAAGCGAGTAACATCGG\tGCAGAAGCGAGTAACATCGG\t1\n+GTGTCAATGGTCCATCGTTT\tGTGTCAATGGTCCATCGTTT\t1\n+TTATCGGCAGGCTATCCGTT\tTTATCGGCAGGCTATCCGTT\t3\n+CTGGAGTCACCCTACGTTGG\tCTGGAGTCACCCTACGTTGG\t1\n+TGCGTCAGTCGCCTACCTGT\tTGCGTCAGTCGCCTACCTGT\t1\n+GCTCACTCTCGCTCCTGTTT\tGCTCACTCTCGCTCCTGTTT\t1\n+CCAGTATTCCCACAATTTTA\tCCAGTATTCCCACAATTTTA\t1\n+CACTGTCCTGCACCGCCGTT\tCACTGTCCTGCACCGCCGTT\t1\n+AGATCATTGCTTTAGAGCTA\tAGATCATTGCTTTAGAGCTA\t1\n+CGATGACGTTACCGCAGCCG\tCGATGACGTTACCGCAGCCG\t1\n+ACCTTAATATTTGCCTCATC\tACCTTAATATTTGCCTCATC\t1\n+GCTGCGAGCCCGTTATCAAC\tGCTGCGAGCCCGTTATCAAC\t4\n+TCCGGACGGTCTGCACGTGT\tTCCGGACGGTCTGCACGTGT\t2\n+CCCGTTTTAGTCTCAGCCCC\tCCCGTTTTAGTCTCAGCCCC\t1\n+GCTGTAGATCCGTCCGTCAG\tGCTGTAGATCCGTCCGTCAG\t1\n+TCGCTGTTCTCCTCCGTTTT\tTCGCTGTTCTCCTCCGTTTT\t1\n+TTGCCCTACAGATGCCTTCT\tTTGCCCTACAGATGCCTTCT\t1\n+CCACGGTGTTCATGCATGTG\tCCACGGTGTTCATGCATGTG\t1\n+GATGGCATCAATGAATCGCG\tGATGGCATCAATGAATCGCG\t1\n+CGGAGGCTCAAACATCGCGT\tCGGAGGCTCAAACATCGCGT\t2\n+ATTCCTTATCCCACCATCTC\tATTCCTTATCCCACCATCTC\t1\n+GGTTTACAACTTGGAAAAGT\tGGTTTACAACTTGGAAAAGT\t1\n+GTCAGACCTAGTACCGCTAG\tGTCAGACCTAGTACCGCTAG\t1\n+CGTACGACCTGACAGCCAAG\tCGTACGACCTGACAGCCAAG\t1\n+GGCACGTTATCAACTTGAAA\tGGCACGTTATCAACTTGAAA\t5\n+TCACGACGCCGCCCCACTGG\tTCACGACGCCGCCCCACTGG\t1\n+CTATAACCACCTAGTACGTT\tCTATAACCACCTAGTACGTT\t1\n+CAACAAGCCATCAACGCCAG\tCAACAAGCCATCAACGCCAG\t1\n+TGACCAGCCACGCCCCTACA\tTGACCAGCCACGCCCCTACA\t1\n+TTGCAGGTCATTTGATCTTG\tTTGCAGGTCATTTGATCTTG\t1\n+TCGAAACCACAGATGTCCCT\tTCGAAACCACAGATGTCCCT\t1\n+TCTGTACGCGTGTTCCTTTG\tTCTGTACGCGTGTTCCTTTG\t1\n+CAGACACAGCGAGTCCGTTA\tCAGACACAGCGAGTCCGTTA\t2\n+ACCTGCAACGCTGCGCGCCG\tACCTGCAACGCTGCGCGCCG\t1\n+GCTCGAGCAGCACACATCAT\tGCTCGAGCAGCACACATCAT\t1\n+ACGGGCTTGCCTTGACAAGT\tACGGGCTTGCCTTGACAAGT\t1\n+TTTTCCGATTCCCGAGACCG\tTTTTCCGATTCCCGAGACCG\t1\n+CCGAGCGCATATGCCCAAGT\tCCGAGCGCATATGCCCAAGT\t1\n+AACCTTCAAAGGCCATCGTT\tAACCTTCAAAGGCCATCGTT\t1\n+ATATCGCTCTATTCTCCATG'..b'GAATAAACGTTGATA\tTAAGCGAATAAACGTTGATA\t1\n+CGACTCTTGGCCAATCCCCT\tCGACTCTTGGCCAATCCCCT\t1\n+GCACTCAGTGCGAAGTCAAG\tGCACTCAGTGCGAAGTCAAG\t1\n+ACACTGCGTGTCTCCCAGTT\tACACTGCGTGTCTCCCAGTT\t1\n+CCGTCCCACCCGACTTATTG\tCCGTCCCACCCGACTTATTG\t1\n+TGTCACAGTTCTCGCAGCAC\tTGTCACAGTTCTCGCAGCAC\t1\n+CGCCAGCATCGAAAGGTACG\tCGCCAGCATCGAAAGGTACG\t1\n+GCAGCCGTATCAACTTGAAA\tGCAGCCGTATCAACTTGAAA\t1\n+CAATGGCGGCAGTCGCGCTC\tCAATGGCGGCAGTCGCGCTC\t1\n+TTTACATGACTTCCAAGCGT\tTTTACATGACTTCCAAGCGT\t1\n+GTGTCCGTTCTCAACTTGAA\tGTGTCCGTTCTCAACTTGAA\t1\n+CATTGGGCATTAACATTTGT\tCATTGGGCATTAACATTTGT\t1\n+ACCGAACATGCGATCCTCCG\tACCGAACATGCGATCCTCCG\t1\n+GTTGCACGTAAACTTGCGCG\tGTTGCACGTAAACTTGCGCG\t1\n+TGCAAACTTACCTGGGGCTG\tTGCAAACTTACCTGGGGCTG\t1\n+CGGCATTCATGTACCTCCAG\tCGGCATTCATGTACCTCCAG\t1\n+TCTGGCAGGCCACCGTTTAG\tTCTGGCAGGCCACCGTTTAG\t1\n+TCCGTTTTTTCGAGTTTAGA\tTCCGTTTTTTCGAGTTTAGA\t1\n+TGCGATGCTGACGTTCTCAG\tTGCGATGCTGACGTTCTCAG\t1\n+TCGCCAAGAGGGCGCAATCG\tTCGCCAAGAGGGCGCAATCG\t1\n+TAACAGAGTTTGCCATGCTC\tTAACAGAGTTTGCCATGCTC\t1\n+CAGCGCTAGCAGACCAGGTT\tCAGCGCTAGCAGACCAGGTT\t1\n+CGTCACATCATTCAACTCCT\tCGTCACATCATTCAACTCCT\t1\n+AACGAAACACCTTCCGATGT\tAACGAAACACCTTCCGATGT\t1\n+TCACCCGCTCATCAAATCCG\tTCACCCGCTCATCAAATCCG\t1\n+CTGACCGCCCTTAGCGTTAG\tCTGACCGCCCTTAGCGTTAG\t1\n+AACACCTGTCAGCCGACTGT\tAACACCTGTCAGCCGACTGT\t1\n+AATTCCTCTAGAGCTAGAAA\tAATTCCTCTAGAGCTAGAAA\t1\n+TCCGGCAGCAGGTCTCGTAC\tTCCGGCAGCAGGTCTCGTAC\t1\n+ACCGGCAGAAGCCCCTGACG\tACCGGCAGAAGCCCCTGACG\t1\n+GCGCCCTGCGCTCCTTCCGC\tGCGCCCTGCGCTCCTTCCGC\t1\n+AACTACTGACGTCGCACTAG\tAACTACTGACGTCGCACTAG\t1\n+GCCCTGAGAGACACCGCCTG\tGCCCTGAGAGACACCGCCTG\t1\n+TTCAAGCAAGTTAAAATAAG\tTTCAAGCAAGTTAAAATAAG\t1\n+TGGGCACTGACCGTGGGCAG\tTGGGCACTGACCGTGGGCAG\t1\n+TGAAGTCCTGCGCGATCTCA\tTGAAGTCCTGCGCGATCTCA\t1\n+TCGCGACTACAGAGCCCCGG\tTCGCGACTACAGAGCCCCGG\t1\n+CAGCGCCATCTCGTAGGTTA\tCAGCGCCATCTCGTAGGTTA\t1\n+ACCTCCAGGCTGGTCCGTTA\tACCTCCAGGCTGGTCCGTTA\t1\n+AGCGACTACGACTCTGTTGT\tAGCGACTACGACTCTGTTGT\t1\n+TGAAGATTGAAAGTTAAAAT\tTGAAGATTGAAAGTTAAAAT\t1\n+CTTGTCTCAAATCAGCTACT\tCTTGTCTCAAATCAGCTACT\t1\n+GCGTTTTCATCCTGTCGCGT\tGCGTTTTCATCCTGTCGCGT\t1\n+ACTTGAGACAAGCTCTCGCG\tACTTGAGACAAGCTCTCGCG\t1\n+TCCAAAACTCCTTTACCAAG\tTCCAAAACTCCTTTACCAAG\t1\n+TGACCTCGACTGCTCTGACG\tTGACCTCGACTGCTCTGACG\t1\n+TCTTGTTCCGATACACCGAT\tTCTTGTTCCGATACACCGAT\t1\n+ACGCTTGAGCGACGAGACCG\tACGCTTGAGCGACGAGACCG\t1\n+ACGCCCAGCGCTCGTACACT\tACGCCCAGCGCTCGTACACT\t1\n+TTGCGAATGTTACCATCTCG\tTTGCGAATGTTACCATCTCG\t1\n+ATAACGTGATCACAGACATG\tATAACGTGATCACAGACATG\t1\n+ACGAGATGTCCCGCGCCAGG\tACGAGATGTCCCGCGCCAGG\t1\n+AACTCGCGATGCCATCTCCG\tAACTCGCGATGCCATCTCCG\t1\n+TGTTCTCCTGCGCCTGCACG\tTGTTCTCCTGCGCCTGCACG\t1\n+ACTTACCAACTACCATATGT\tACTTACCAACTACCATATGT\t1\n+AACCACTGCGTCCTTATCGT\tAACCACTGCGTCCTTATCGT\t1\n+CTGTGACCCATGCCTCCAAG\tCTGTGACCCATGCCTCCAAG\t1\n+TAGCTTACTCGAAATTCACG\tTAGCTTACTCGAAATTCACG\t1\n+GTGACTCCAGCTATCCAGGT\tGTGACTCCAGCTATCCAGGT\t1\n+TACTCACTCGGCAAACTTGG\tTACTCACTCGGCAAACTTGG\t1\n+CTTCAAGTTAAAATACGGCT\tCTTCAAGTTAAAATACGGCT\t1\n+ACCCCATCCTTTCATTGAGG\tACCCCATCCTTTCATTGAGG\t1\n+ACACCATTTAGAGCGTGTCT\tACACCATTTAGAGCGTGTCT\t1\n+TAGGCTTACCTAGTTTGCAC\tTAGGCTTACCTAGTTTGCAC\t1\n+CTCCAGAACCTGCCCAAGAA\tCTCCAGAACCTGCCCAAGAA\t1\n+TTTTCCAGCACTCGAATGCG\tTTTTCCAGCACTCGAATGCG\t1\n+TCCCGCGAGACGAGCTGCGT\tTCCCGCGAGACGAGCTGCGT\t1\n+CTCGTCGCAGCCCACAAGCA\tCTCGTCGCAGCCCACAAGCA\t1\n+CCCCCCCACTCTCAACGGGT\tCCCCCCCACTCTCAACGGGT\t1\n+TCTCTCCGCGCAGCAACTCA\tTCTCTCCGCGCAGCAACTCA\t1\n+GCTTACATAGCAAATCTATG\tGCTTACATAGCAAATCTATG\t1\n+TCTGGTGATCTCTCCCGAGT\tTCTGGTGATCTCTCCCGAGT\t1\n+CCGTATCCCCACTCTCATCA\tCCGTATCCCCACTCTCATCA\t1\n+ACATTTGTTCTGCAAAGACA\tACATTTGTTCTGCAAAGACA\t1\n+ATCATGTTTCTAAAGCCGCG\tATCATGTTTCTAAAGCCGCG\t1\n+TTCCAAAATACGACTTTACC\tTTCCAAAATACGACTTTACC\t1\n+ATCAGTTTTAGAGCTAGAAA\tATCAGTTTTAGAGCTAGAAA\t1\n+CCGTTCTCAACTTGAAAAAG\tCCGTTCTCAACTTGAAAAAG\t1\n+CTGCATATCTTCGAGATGCT\tCTGCATATCTTCGAGATGCT\t1\n+ACTTCGACACGACCTCTACG\tACTTCGACACGACCTCTACG\t1\n+CTCCTCGAAGGGCCGACACG\tCTCCTCGAAGGGCCGACACG\t1\n+TGTGTACAGCTCTCCTCCGT\tTGTGTACAGCTCTCCTCCGT\t1\n+CTTATAGTCAGCCTCAAAAG\tCTTATAGTCAGCCTCAAAAG\t1\n+TCCGCAGCCCTCCACACGTA\tTCCGCAGCCCTCCACACGTA\t1\n+CCCGATGATCTCGATGCCCG\tCCCGATGATCTCGATGCCCG\t1\n+TCTCCTATCAAGGCCATTCT\tTCTCCTATCAAGGCCATTCT\t1\n+AGGATCCTGAGACGTTCCTC\tAGGATCCTGAGACGTTCCTC\t1\n+TTCCCTATCAGTAGAAATAG\tTTCCCTATCAGTAGAAATAG\t1\n+CTGGTCTCGCCGGTAGCTAT\tCTGGTCTCGCCGGTAGCTAT\t1\n' |
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diff -r 000000000000 -r b8da4d41aa1d test-data/out.countsummary.pdf |
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Binary file test-data/out.countsummary.pdf has changed |
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diff -r 000000000000 -r b8da4d41aa1d test-data/out.countsummary.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/out.countsummary.txt Wed Feb 14 06:42:55 2018 -0500 |
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@@ -0,0 +1,2 @@ +File Label Reads Mapped Percentage TotalsgRNAs Zerocounts GiniIndex NegSelQC NegSelQCPval NegSelQCPvalPermutation NegSelQCPvalPermutationFDR NegSelQCGene +input.gz sample1 2500 1453 0.5812 2550 1276 0.5267 0 1 1 1 0.0 |
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diff -r 000000000000 -r b8da4d41aa1d test-data/out.mageckGSEA.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/out.mageckGSEA.txt Wed Feb 14 06:42:55 2018 -0500 |
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@@ -0,0 +1,2 @@ +Pathway Size ES p p_permutation FDR Ranking Hits LFC +KEGG_RIBOSOME 88 1 0 0 0 1 0 0 |
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diff -r 000000000000 -r b8da4d41aa1d test-data/out.mle.gene_summary.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/out.mle.gene_summary.txt Wed Feb 14 06:42:55 2018 -0500 |
b |
b'@@ -0,0 +1,101 @@\n+Gene\tsgRNA\tHL60|beta\tHL60|z\tHL60|p-value\tHL60|fdr\tHL60|wald-p-value\tHL60|wald-fdr\tKBM7|beta\tKBM7|z\tKBM7|p-value\tKBM7|fdr\tKBM7|wald-p-value\tKBM7|wald-fdr\n+A1CF\t10\t0.21726\t2.3501\t0.09\t0.098901\t0.018771\t0.045548\t0.22205\t2.4019\t0.11\t0.13258\t0.016311\t0.044445\n+AAAS\t10\t0.20562\t2.038\t0.108\t0.11368\t0.041547\t0.048311\t0.22245\t2.2047\t0.11\t0.13258\t0.027472\t0.044445\n+AAK1\t10\t0.25639\t2.1126\t0.054\t0.07\t0.034633\t0.045548\t0.31519\t2.5972\t0.026\t0.092857\t0.0093995\t0.044445\n+AATF\t10\t0.26019\t2.137\t0.048\t0.07\t0.032596\t0.045548\t0.17209\t1.4898\t0.202\t0.20612\t0.13627\t0.14195\n+AATK\t10\t0.37034\t2.5201\t0.012\t0.07\t0.011733\t0.045548\t0.32484\t2.2105\t0.024\t0.088889\t0.027074\t0.044445\n+ABCB8\t10\t0.25311\t2.4583\t0.054\t0.07\t0.013958\t0.045548\t0.2626\t2.5505\t0.058\t0.10345\t0.010756\t0.044445\n+ABCC1\t10\t0.20905\t2.0722\t0.106\t0.11277\t0.038249\t0.045548\t0.2381\t2.3602\t0.086\t0.10886\t0.018265\t0.044445\n+ABCF1\t10\t0.26857\t2.0808\t0.046\t0.07\t0.037453\t0.045548\t0.2407\t1.8649\t0.082\t0.10886\t0.062201\t0.069889\n+ABHD14B\t10\t0.25291\t2.1394\t0.054\t0.07\t0.032402\t0.045548\t0.24531\t2.0751\t0.076\t0.10857\t0.037979\t0.049559\n+ABI1\t10\t0.28136\t2.2952\t0.038\t0.07\t0.021724\t0.045548\t0.23619\t1.9267\t0.086\t0.10886\t0.054023\t0.062096\n+ABL1\t10\t0.35653\t2.8826\t0.014\t0.07\t0.0039444\t0.045548\t0.19033\t1.5389\t0.172\t0.17938\t0.12383\t0.13174\n+ABL2\t10\t0.31786\t2.537\t0.026\t0.07\t0.011181\t0.045548\t0.28849\t2.3026\t0.044\t0.096154\t0.0213\t0.044445\n+ABLIM2\t10\t0.33199\t2.1246\t0.024\t0.07\t0.033624\t0.045548\t0.35781\t2.2898\t0.016\t0.088889\t0.022035\t0.044445\n+ABT1\t10\t0.64492\t1.2807\t0\t0\t0.20031\t0.20031\t0.56048\t1.113\t0\t0\t0.26572\t0.26572\n+ABTB1\t10\t0.25881\t2.2156\t0.048\t0.07\t0.026717\t0.045548\t0.27981\t2.3954\t0.048\t0.096154\t0.016601\t0.044445\n+ACAD11\t10\t0.2457\t2.1273\t0.056\t0.07\t0.033397\t0.045548\t0.26753\t2.3163\t0.052\t0.098113\t0.020539\t0.044445\n+ACAD9\t10\t0.28033\t2.3396\t0.038\t0.07\t0.019306\t0.045548\t0.23942\t1.9982\t0.084\t0.10886\t0.045699\t0.055059\n+ACAT2\t10\t0.33017\t1.9472\t0.024\t0.07\t0.051509\t0.056401\t0.34801\t2.0524\t0.016\t0.088889\t0.040129\t0.049805\n+ACBD6\t10\t0.26005\t2.4829\t0.048\t0.07\t0.013031\t0.045548\t0.22982\t2.1942\t0.094\t0.1175\t0.028219\t0.044707\n+ACD\t10\t0.29956\t2.263\t0.028\t0.07\t0.023633\t0.045548\t0.30306\t2.2895\t0.036\t0.096154\t0.022052\t0.044445\n+ACHE\t10\t0.26039\t1.9394\t0.048\t0.07\t0.052453\t0.056401\t0.27459\t2.0451\t0.05\t0.096154\t0.04084\t0.049805\n+ACIN1\t10\t0.24992\t1.6773\t0.054\t0.07\t0.093484\t0.097379\t0.16332\t1.1553\t0.222\t0.22424\t0.24796\t0.25047\n+ACLY\t10\t0.64139\t1.3277\t0\t0\t0.18428\t0.18614\t0.56404\t1.1676\t0\t0\t0.24298\t0.24794\n+ACO2\t10\t0.26736\t2.3551\t0.048\t0.07\t0.018518\t0.045548\t0.24404\t2.1496\t0.078\t0.10886\t0.031586\t0.045123\n+ACP1\t10\t0.30066\t2.0818\t0.028\t0.07\t0.037363\t0.045548\t0.32268\t2.2343\t0.024\t0.088889\t0.025465\t0.044445\n+ACRC\t10\t0.316\t2.1738\t0.026\t0.07\t0.029722\t0.045548\t0.32721\t2.2508\t0.024\t0.088889\t0.024396\t0.044445\n+ACSL6\t10\t0.30715\t2.5085\t0.028\t0.07\t0.012125\t0.045548\t0.27154\t2.2176\t0.05\t0.096154\t0.026579\t0.044445\n+ACSS2\t10\t0.27125\t2.396\t0.044\t0.07\t0.016573\t0.045548\t0.31578\t2.7894\t0.024\t0.088889\t0.0052812\t0.044445\n+ACTL6A\t10\t0.23154\t2.2408\t0.072\t0.085714\t0.025038\t0.045548\t0.20097\t1.945\t0.138\t0.14681\t0.051771\t0.060199\n+ACTL6B\t10\t0.30195\t2.3158\t0.028\t0.07\t0.020569\t0.045548\t0.29791\t2.2849\t0.036\t0.096154\t0.02232\t0.044445\n+ACTL7A\t10\t0.22531\t2.1897\t0.078\t0.089655\t0.028544\t0.045548\t0.2423\t2.3549\t0.082\t0.10886\t0.018527\t0.044445\n+ACTN1\t10\t0.30451\t2.4532\t0.028\t0.07\t0.014161\t0.045548\t0.27462\t2.2124\t0.05\t0.096154\t0.026942\t0.044445\n+ACTN4\t10\t0.3039\t2.2464\t0.028\t0.07\t0.024679\t0.045548\t0.33156\t2.4509\t0.022\t0.088889\t0.01425\t0.044445\n+ACTR1A\t10\t0.28127\t2.1093\t0.038\t0.07\t0.034915\t0.045548\t0.30952\t2.3212\t0.028\t0.093333\t0.020274\t0.044445\n+ACTR3\t10\t0.28223\t2.1469\t0.038\t0.07\t0.031799\t0.045548\t0.25252\t1.9209\t0.068\t0.10462\t0.054741\t0.062206\n+ACTR5\t10\t0.2754\t2.7989\t0.04\t0.07\t0.0051271\t0.045548\t0.20514\t2.0849\t0.136\t0.14624\t0.037074\t0.049433\n+ACTR8\t10\t0.23674\t1.6117\t0.06\t0.074074\t0.10703\t0.10922\t0.21097\t1.4362\t0.122\t0.13407\t0.15095\t0.15562\n+ACTRT3\t10\t0.2132\t1.984\t0.096\t0.10435\t0.047252\t0.053696\t0.25182\t2.3435\t0.068\t0.10462\t0.019105\t0.044445\n+ACVR1B\t10\t0.28652\t2.2884\t0.034\t0.'..b'028\t0.07\t0.037665\t0.045548\t0.3371\t2.2368\t0.02\t0.088889\t0.025302\t0.044445\n+ADH7\t10\t0.23185\t2.1764\t0.072\t0.085714\t0.029529\t0.045548\t0.25313\t2.3761\t0.068\t0.10462\t0.017497\t0.044445\n+ADI1\t10\t0.26344\t2.2066\t0.048\t0.07\t0.027345\t0.045548\t0.2966\t2.4843\t0.038\t0.096154\t0.012981\t0.044445\n+ADIRF\t10\t0.23157\t2.1952\t0.072\t0.085714\t0.028148\t0.045548\t0.21758\t2.0626\t0.118\t0.13258\t0.039152\t0.049559\n+ADK\t10\t0.25823\t2.3541\t0.05\t0.07\t0.018569\t0.045548\t0.25466\t2.3215\t0.066\t0.10462\t0.020258\t0.044445\n+ADNP2\t10\t0.19944\t2.0931\t0.12\t0.12245\t0.036336\t0.045548\t0.25778\t2.7054\t0.06\t0.10345\t0.0068216\t0.044445\n+ADNP\t10\t0.33596\t1.9412\t0.024\t0.07\t0.052236\t0.056401\t0.44376\t2.5641\t0\t0\t0.010346\t0.044445\n+ADPRHL2\t10\t0.26332\t2.3044\t0.048\t0.07\t0.021202\t0.045548\t0.27219\t2.382\t0.05\t0.096154\t0.017218\t0.044445\n+ADRA1A\t10\t0.26321\t2.4075\t0.048\t0.07\t0.016062\t0.045548\t0.24245\t2.2176\t0.08\t0.10886\t0.026583\t0.044445\n+ADRA1B\t10\t0.26869\t2.4689\t0.046\t0.07\t0.013554\t0.045548\t0.24756\t2.2747\t0.076\t0.10857\t0.022923\t0.044445\n+ADRB1\t10\t0.26113\t2.1522\t0.048\t0.07\t0.031385\t0.045548\t0.25076\t2.0666\t0.068\t0.10462\t0.038768\t0.049559\n+ADRB2\t10\t0.3434\t2.0721\t0.018\t0.07\t0.038261\t0.045548\t0.32418\t1.9561\t0.024\t0.088889\t0.050457\t0.059361\n+ADRB3\t10\t0.27356\t2.3431\t0.042\t0.07\t0.019123\t0.045548\t0.26345\t2.1408\t0.056\t0.10345\t0.032287\t0.045408\n+ADRBK1\t10\t0.39621\t2.5298\t0.006\t0.07\t0.011414\t0.045548\t0.38361\t2.4493\t0.004\t0.057143\t0.014314\t0.044445\n+ADRBK2\t10\t0.311\t2.4196\t0.028\t0.07\t0.015537\t0.045548\t0.30224\t2.3515\t0.036\t0.096154\t0.018698\t0.044445\n+ADRM1\t10\t0.19795\t2.0633\t0.12\t0.12245\t0.039089\t0.045987\t0.21217\t2.2114\t0.122\t0.13407\t0.027005\t0.044445\n+AEBP1\t10\t0.30109\t2.094\t0.028\t0.07\t0.036256\t0.045548\t0.31995\t2.2252\t0.024\t0.088889\t0.026067\t0.044445\n+AEBP2\t10\t0.25078\t2.3902\t0.054\t0.07\t0.016839\t0.045548\t0.25977\t2.4758\t0.06\t0.10345\t0.013293\t0.044445\n+AEN\t10\t0.31767\t2.5501\t0.026\t0.07\t0.010771\t0.045548\t0.21703\t1.7422\t0.118\t0.13258\t0.081467\t0.089524\n+AES\t10\t0.27613\t2.5026\t0.04\t0.07\t0.01233\t0.045548\t0.28435\t2.5771\t0.048\t0.096154\t0.0099638\t0.044445\n+AFAP1L2\t10\t0.27787\t2.5825\t0.038\t0.07\t0.0098091\t0.045548\t0.25248\t2.3465\t0.068\t0.10462\t0.018949\t0.044445\n+AFF1\t10\t0.24186\t2.0236\t0.056\t0.07\t0.043007\t0.049434\t0.28577\t2.391\t0.044\t0.096154\t0.0168\t0.044445\n+AFF2\t10\t0.22079\t1.9621\t0.086\t0.095556\t0.049749\t0.055889\t0.28962\t2.5737\t0.044\t0.096154\t0.010061\t0.044445\n+AFF3\t10\t0.22476\t2.1578\t0.078\t0.089655\t0.030942\t0.045548\t0.21756\t2.0887\t0.118\t0.13258\t0.03673\t0.049433\n+AFF4\t10\t0.31149\t2.1142\t0.028\t0.07\t0.034496\t0.045548\t0.3627\t2.4618\t0.012\t0.088889\t0.013825\t0.044445\n+AFMID\t10\t0.22778\t2.1955\t0.078\t0.089655\t0.02813\t0.045548\t0.22159\t2.1358\t0.112\t0.13258\t0.032694\t0.045408\n+AFTPH\t10\t0.25053\t2.1997\t0.054\t0.07\t0.027828\t0.045548\t0.24929\t2.1888\t0.072\t0.10746\t0.028613\t0.044707\n+AGAP2\t10\t0.3135\t2.4258\t0.028\t0.07\t0.015274\t0.045548\t0.27805\t2.1516\t0.048\t0.096154\t0.031432\t0.045123\n+AGAP3\t10\t0.27682\t2.4409\t0.04\t0.07\t0.01465\t0.045548\t0.31626\t2.7887\t0.024\t0.088889\t0.0052921\t0.044445\n+AGBL5\t10\t0.18851\t1.7706\t0.146\t0.14747\t0.076634\t0.080668\t0.25487\t2.3938\t0.066\t0.10462\t0.016673\t0.044445\n+AGFG1\t10\t0.26133\t2.2912\t0.048\t0.07\t0.021954\t0.045548\t0.24813\t2.0639\t0.074\t0.10857\t0.039025\t0.049559\n+AGL\t10\t0.65242\t1.6625\t0\t0\t0.096417\t0.099399\t0.60099\t1.5314\t0\t0\t0.12566\t0.13228\n+AGPAT3\t10\t0.20246\t2.1377\t0.112\t0.11667\t0.03254\t0.045548\t0.18609\t1.9649\t0.174\t0.17938\t0.049424\t0.058838\n+AGPAT5\t10\t0.32784\t1.8786\t0.024\t0.07\t0.060303\t0.064152\t0.31917\t1.8289\t0.024\t0.088889\t0.067418\t0.074909\n+AGTPBP1\t10\t0.25153\t2.1735\t0.054\t0.07\t0.029741\t0.045548\t0.29805\t2.5755\t0.036\t0.096154\t0.01001\t0.044445\n+AHCTF1\t10\t0.24987\t2.2694\t0.054\t0.07\t0.023245\t0.045548\t0.18875\t1.7143\t0.174\t0.17938\t0.086471\t0.09399\n+AHCY\t10\t0.25511\t1.9574\t0.054\t0.07\t0.0503\t0.055889\t0.20591\t1.5799\t0.136\t0.14624\t0.11413\t0.12272\n+AHNAK2\t10\t0.27116\t2.5067\t0.044\t0.07\t0.012185\t0.045548\t0.27693\t2.5601\t0.048\t0.096154\t0.010466\t0.044445\n+AHNAK\t10\t0.22102\t2.4775\t0.084\t0.094382\t0.01323\t0.045548\t0.21459\t2.4055\t0.118\t0.13258\t0.016151\t0.044445\n+AHRR\t9\t0.19687\t2.5399\t0.15\t0.15\t0.011087\t0.045548\t0.18565\t2.2584\t0.226\t0.226\t0.023923\t0.044445\n' |
b |
diff -r 000000000000 -r b8da4d41aa1d test-data/out.mle.log.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/out.mle.log.txt Wed Feb 14 06:42:55 2018 -0500 |
[ |
b'@@ -0,0 +1,749 @@\n+INFO @ Mon, 12 Feb 2018 21:46:26: Parameters: /home/maria/miniconda3/envs/mulled-v1-b1e6893952d2d7e4d51a13d040adcd7bea052bfdc57a24d59056014db875e749/bin/mageck mle -k /tmp/tmpRFR3vr/files/000/dataset_1.dat -d /tmp/tmpRFR3vr/files/000/dataset_2.dat -n output --norm-method median --genes-varmodeling 1000 --permutation-round 10 --adjust-method fdr --threads 1 \n+INFO @ Mon, 12 Feb 2018 21:46:27: Cannot parse design matrix as a string; try to parse it as a file name ... \n+INFO @ Mon, 12 Feb 2018 21:46:27: Design matrix: \n+INFO @ Mon, 12 Feb 2018 21:46:27: [[1. 0. 0.] \n+INFO @ Mon, 12 Feb 2018 21:46:27: [1. 0. 0.] \n+INFO @ Mon, 12 Feb 2018 21:46:27: [1. 1. 0.] \n+INFO @ Mon, 12 Feb 2018 21:46:27: [1. 0. 1.]] \n+INFO @ Mon, 12 Feb 2018 21:46:27: Beta labels:baseline,HL60,KBM7 \n+INFO @ Mon, 12 Feb 2018 21:46:27: Included samples:HL60.initial,KBM7.initial,HL60.final,KBM7.final \n+INFO @ Mon, 12 Feb 2018 21:46:27: Loaded samples:HL60.initial;KBM7.initial;HL60.final;KBM7.final \n+INFO @ Mon, 12 Feb 2018 21:46:27: Sample index: 0;1;2;3 \n+INFO @ Mon, 12 Feb 2018 21:46:27: Loaded 100 genes. \n+DEBUG @ Mon, 12 Feb 2018 21:46:27: Initial (total) size factor: 1.6654412961322171 2.025116092944715 0.720025234995028 0.6592307725342162 \n+DEBUG @ Mon, 12 Feb 2018 21:46:27: Median factor: 1.4705915949061357 1.773663484525946 0.6311218804203026 0.5731039100868989 \n+INFO @ Mon, 12 Feb 2018 21:46:27: Final size factor: 1.4705915949061357 1.773663484525946 0.6311218804203026 0.5731039100868989 \n+INFO @ Mon, 12 Feb 2018 21:46:27: size factor: 0.6799984465189518,0.5638048078028025,1.5844800046134337,1.744884273862957 \n+INFO @ Mon, 12 Feb 2018 21:46:27: Thread 0 started. \n+INFO @ Mon, 12 Feb 2018 21:46:27: Thread 0: total 100 instances. \n+INFO @ Mon, 12 Feb 2018 21:46:27: Thread 0: Calculating AAAS (1) ... \n+INFO @ Mon, 12 Feb 2018 21:46:27: Thread 0 completed. \n+INFO @ Mon, 12 Feb 2018 21:46:27: All threads completed. \n+INFO @ Mon, 12 Feb 2018 21:46:27: Modeling the mean and variance ... \n+INFO @ Mon, 12 Feb 2018 21:46:27: Linear regression: y=-0.902175602829449x+14.539230037913281 \n+INFO @ Mon, 12 Feb 2018 21:46:27: Run the algorithm for the second time ... \n+WARNING @ Mon, 12 Feb 2018 21:46:27: A1CF: beta value does not converge. Try to increase the value of alpha .. \n+WARNING @ Mon, 12 Feb 2018 21:46:27: A1CF: alpha: 0.01 \n+WARNING @ Mon, 12 Feb 2018 21:46:28: A1CF: reaches the maximum number of iterations. \n+WARNING @ Mon, 12 Feb 2018 21:46:28: A1CF: alpha: 0.03 \n+WARNING @ Mon, 12 Feb 2018 21:46:29: A1CF: reaches the maximum number of iterations. \n+WARNING @ Mon, 12 Feb 2018 21:46:29: A1CF: alpha: 0.09 \n+INFO @ Mon, 12 Feb 2018 21:46:29: Calculating AAAS (1) ... \n+WARNING @ Mon, 12 Feb 2018 21:46:29: AAAS: beta value does not converge. Try to increase the value of alpha .. \n+WARNING @ Mon, 12 Feb 2018 21:46:29: AAAS: alpha: 0.01 \n+WARNING @ Mon, 12 Feb 2018 21:46:30: AAAS: reaches the maximum number of iterations. \n+WARNING @ Mon, 12 Feb 2018 21:46:30: AAAS: alpha: 0.03 \n+WARNING @ Mon, 12 Feb 2018 21:46:31: AAAS: reaches the maximum number of iterations. \n+WARNING @ Mon, 12 Feb 2018 21:46:31: AAAS: alpha: 0.09 \n+WARNING @ Mon, 12 Feb 2018 21:46:31: AAK1: beta value does not converge. Try to increase the value of alpha .. \n+WARNING @ Mon, 12 Feb 2018 21:46:31: AAK1: alpha: 0.01 \n+WARNING @ Mon, 12 Feb 2018 21:46:31: AAK1: reaches the maximum number of iterations. \n+WARNING @ Mon, 12 Feb 2018 21:46:31: AAK1: alpha: 0.03 \n+WARNING @ Mon, 12 Feb 2018 21:46:32: AAK1: reaches the maximum number of iterations. \n+WARNING @ Mon, 12 Feb 2018 21:46:32: AAK1: alpha: 0.09 \n+WARNING @ Mon, 12 Feb 2018 21:46:32: AATF: beta value does not converge. Try to increase the value of alpha .. \n+WARNING @ Mon, 12 Feb 2018 21:46:32: AATF: alpha: 0.01 \n+WARNING @ Mon, 12 Feb 2018 21:46:33: AATF: reaches the maximum number of iterations. \n+WARNING @ Mon, 12 Feb 2018 21:46:33: AATF: alpha: 0.03 \n+WARNING @ Mon, 12 Feb 2018 21:46:33: A'..b'1:49:12: Collecting 999 sgRNAs from 100 genes. \n+INFO @ Mon, 12 Feb 2018 21:49:12: Permuting round 0 ... \n+INFO @ Mon, 12 Feb 2018 21:49:12: Thread 0 started. \n+INFO @ Mon, 12 Feb 2018 21:49:12: Thread 0: total 100 instances. \n+INFO @ Mon, 12 Feb 2018 21:49:12: Thread 0: Calculating AAAS (1) ... \n+INFO @ Mon, 12 Feb 2018 21:49:13: Thread 0 completed. \n+INFO @ Mon, 12 Feb 2018 21:49:13: All threads completed. \n+INFO @ Mon, 12 Feb 2018 21:49:13: Permuting round 1 ... \n+INFO @ Mon, 12 Feb 2018 21:49:13: Thread 0 started. \n+INFO @ Mon, 12 Feb 2018 21:49:13: Thread 0: total 100 instances. \n+INFO @ Mon, 12 Feb 2018 21:49:13: Thread 0: Calculating AAAS (1) ... \n+INFO @ Mon, 12 Feb 2018 21:49:14: Thread 0 completed. \n+INFO @ Mon, 12 Feb 2018 21:49:14: All threads completed. \n+INFO @ Mon, 12 Feb 2018 21:49:14: Permuting round 2 ... \n+INFO @ Mon, 12 Feb 2018 21:49:14: Thread 0 started. \n+INFO @ Mon, 12 Feb 2018 21:49:14: Thread 0: total 100 instances. \n+INFO @ Mon, 12 Feb 2018 21:49:14: Thread 0: Calculating AAAS (1) ... \n+INFO @ Mon, 12 Feb 2018 21:49:15: Thread 0 completed. \n+INFO @ Mon, 12 Feb 2018 21:49:15: All threads completed. \n+INFO @ Mon, 12 Feb 2018 21:49:15: Permuting round 3 ... \n+INFO @ Mon, 12 Feb 2018 21:49:15: Thread 0 started. \n+INFO @ Mon, 12 Feb 2018 21:49:15: Thread 0: total 100 instances. \n+INFO @ Mon, 12 Feb 2018 21:49:15: Thread 0: Calculating AAAS (1) ... \n+INFO @ Mon, 12 Feb 2018 21:49:16: Thread 0 completed. \n+INFO @ Mon, 12 Feb 2018 21:49:16: All threads completed. \n+INFO @ Mon, 12 Feb 2018 21:49:16: Permuting round 4 ... \n+INFO @ Mon, 12 Feb 2018 21:49:16: Thread 0 started. \n+INFO @ Mon, 12 Feb 2018 21:49:16: Thread 0: total 100 instances. \n+INFO @ Mon, 12 Feb 2018 21:49:16: Thread 0: Calculating AAAS (1) ... \n+INFO @ Mon, 12 Feb 2018 21:49:16: Thread 0 completed. \n+INFO @ Mon, 12 Feb 2018 21:49:16: All threads completed. \n+INFO @ Mon, 12 Feb 2018 21:49:17: Permuting round 5 ... \n+INFO @ Mon, 12 Feb 2018 21:49:17: Thread 0 started. \n+INFO @ Mon, 12 Feb 2018 21:49:17: Thread 0: total 100 instances. \n+INFO @ Mon, 12 Feb 2018 21:49:17: Thread 0: Calculating AAAS (1) ... \n+INFO @ Mon, 12 Feb 2018 21:49:17: Thread 0 completed. \n+INFO @ Mon, 12 Feb 2018 21:49:17: All threads completed. \n+INFO @ Mon, 12 Feb 2018 21:49:18: Permuting round 6 ... \n+INFO @ Mon, 12 Feb 2018 21:49:18: Thread 0 started. \n+INFO @ Mon, 12 Feb 2018 21:49:18: Thread 0: total 100 instances. \n+INFO @ Mon, 12 Feb 2018 21:49:18: Thread 0: Calculating AAAS (1) ... \n+INFO @ Mon, 12 Feb 2018 21:49:19: Thread 0 completed. \n+INFO @ Mon, 12 Feb 2018 21:49:19: All threads completed. \n+INFO @ Mon, 12 Feb 2018 21:49:19: Permuting round 7 ... \n+INFO @ Mon, 12 Feb 2018 21:49:19: Thread 0 started. \n+INFO @ Mon, 12 Feb 2018 21:49:19: Thread 0: total 100 instances. \n+INFO @ Mon, 12 Feb 2018 21:49:19: Thread 0: Calculating AAAS (1) ... \n+INFO @ Mon, 12 Feb 2018 21:49:20: Thread 0 completed. \n+INFO @ Mon, 12 Feb 2018 21:49:20: All threads completed. \n+INFO @ Mon, 12 Feb 2018 21:49:20: Permuting round 8 ... \n+INFO @ Mon, 12 Feb 2018 21:49:20: Thread 0 started. \n+INFO @ Mon, 12 Feb 2018 21:49:20: Thread 0: total 100 instances. \n+INFO @ Mon, 12 Feb 2018 21:49:20: Thread 0: Calculating AAAS (1) ... \n+INFO @ Mon, 12 Feb 2018 21:49:21: Thread 0 completed. \n+INFO @ Mon, 12 Feb 2018 21:49:21: All threads completed. \n+INFO @ Mon, 12 Feb 2018 21:49:21: Permuting round 9 ... \n+INFO @ Mon, 12 Feb 2018 21:49:21: Thread 0 started. \n+INFO @ Mon, 12 Feb 2018 21:49:21: Thread 0: total 100 instances. \n+INFO @ Mon, 12 Feb 2018 21:49:21: Thread 0: Calculating AAAS (1) ... \n+INFO @ Mon, 12 Feb 2018 21:49:21: Thread 0 completed. \n+INFO @ Mon, 12 Feb 2018 21:49:21: All threads completed. \n+INFO @ Mon, 12 Feb 2018 21:49:22: Assigning p values... \n+INFO @ Mon, 12 Feb 2018 21:49:22: Writing gene results to output.gene_summary.txt \n+INFO @ Mon, 12 Feb 2018 21:49:22: Writing sgRNA results to output.sgrna_summary.txt \n' |
b |
diff -r 000000000000 -r b8da4d41aa1d test-data/out.mle.sgrna_summary.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/out.mle.sgrna_summary.txt Wed Feb 14 06:42:55 2018 -0500 |
b |
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|
b |
diff -r 000000000000 -r b8da4d41aa1d test-data/out.pathway.log.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/out.pathway.log.txt Wed Feb 14 06:42:55 2018 -0500 |
b |
@@ -0,0 +1,30 @@ +INFO @ Mon, 12 Feb 2018 04:19:29: Parameters: /home/maria/miniconda3/envs/mulled-v1-b1e6893952d2d7e4d51a13d040adcd7bea052bfdc57a24d59056014db875e749/bin/mageck pathway --gene-ranking /tmp/tmpd9LSrs/files/000/dataset_19.dat --gmt-file /tmp/tmpd9LSrs/files/000/dataset_20.dat -n sample1 --method gsea --sort-criteria neg --ranking-column 2 --ranking-column-2 8 --pathway-alpha 0.25 --permutation 1000 +INFO @ Mon, 12 Feb 2018 04:19:29: Welcome to MAGeCK v0.5.7. Command: pathway +INFO @ Mon, 12 Feb 2018 04:19:29: GSEA command for negative selection: mageckGSEA -s -c 2 -p 1000 -g "/tmp/tmpd9LSrs/files/000/dataset_20.dat" -r "/tmp/tmpd9LSrs/files/000/dataset_19.dat" -o sample1.pathway.low.txt +INFO @ Mon, 12 Feb 2018 04:19:29: Running command: mageckGSEA -s -c 2 -p 1000 -g "/tmp/tmpd9LSrs/files/000/dataset_20.dat" -r "/tmp/tmpd9LSrs/files/000/dataset_19.dat" -o sample1.pathway.low.txt +INFO @ Mon, 12 Feb 2018 04:19:29: Command message: +INFO @ Mon, 12 Feb 2018 04:19:29: GMT file: /tmp/tmpd9LSrs/files/000/dataset_20.dat +INFO @ Mon, 12 Feb 2018 04:19:29: Rank file: /tmp/tmpd9LSrs/files/000/dataset_19.dat +INFO @ Mon, 12 Feb 2018 04:19:29: Output file: sample1.pathway.low.txt +INFO @ Mon, 12 Feb 2018 04:19:29: pathway size:1 +INFO @ Mon, 12 Feb 2018 04:19:29: rank size:101 +INFO @ Mon, 12 Feb 2018 04:19:29: Permutations:1000 +INFO @ Mon, 12 Feb 2018 04:19:29: . +INFO @ Mon, 12 Feb 2018 04:19:29: +INFO @ Mon, 12 Feb 2018 04:19:29: End command message. +INFO @ Mon, 12 Feb 2018 04:19:29: GSEA command for positive selection: mageckGSEA -s -c 8 -p 1000 -g "/tmp/tmpd9LSrs/files/000/dataset_20.dat" -r "/tmp/tmpd9LSrs/files/000/dataset_19.dat" -o sample1.pathway.high.txt +INFO @ Mon, 12 Feb 2018 04:19:29: Running command: mageckGSEA -s -c 8 -p 1000 -g "/tmp/tmpd9LSrs/files/000/dataset_20.dat" -r "/tmp/tmpd9LSrs/files/000/dataset_19.dat" -o sample1.pathway.high.txt +INFO @ Mon, 12 Feb 2018 04:19:29: Command message: +INFO @ Mon, 12 Feb 2018 04:19:29: GMT file: /tmp/tmpd9LSrs/files/000/dataset_20.dat +INFO @ Mon, 12 Feb 2018 04:19:29: Rank file: /tmp/tmpd9LSrs/files/000/dataset_19.dat +INFO @ Mon, 12 Feb 2018 04:19:29: Output file: sample1.pathway.high.txt +INFO @ Mon, 12 Feb 2018 04:19:29: pathway size:1 +INFO @ Mon, 12 Feb 2018 04:19:29: rank size:101 +INFO @ Mon, 12 Feb 2018 04:19:29: Permutations:1000 +INFO @ Mon, 12 Feb 2018 04:19:29: . +INFO @ Mon, 12 Feb 2018 04:19:29: +INFO @ Mon, 12 Feb 2018 04:19:29: End command message. +INFO @ Mon, 12 Feb 2018 04:19:29: Running command: rm sample1.pathway.low.tmp +INFO @ Mon, 12 Feb 2018 04:19:29: Running command: rm sample1.pathway.high.tmp +INFO @ Mon, 12 Feb 2018 04:19:29: Running command: rm sample1.pathway.low.txt +INFO @ Mon, 12 Feb 2018 04:19:29: Running command: rm sample1.pathway.high.txt |
b |
diff -r 000000000000 -r b8da4d41aa1d test-data/out.pathway.pathway_summary.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/out.pathway.pathway_summary.txt Wed Feb 14 06:42:55 2018 -0500 |
b |
@@ -0,0 +1,2 @@ +id num neg|score neg|rra neg|p-value neg|fdr neg|rank neg|goodgene neg|lfc pos|score pos|rra pos|p-value pos|fdr pos|rank pos|goodgene pos|lfc +KEGG_RIBOSOME 88 1 0 0 0 1 0 0 1 0 0 0 1 0 0 |
b |
diff -r 000000000000 -r b8da4d41aa1d test-data/out.test.R --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/out.test.R Wed Feb 14 06:42:55 2018 -0500 |
[ |
b'@@ -0,0 +1,930 @@\n+pdf(file=\'output.pdf\',width=4.5,height=4.5);\n+gstable=read.table(\'output.gene_summary.txt\',header=T)\n+# \n+#\n+# parameters\n+# Do not modify the variables beginning with "__"\n+\n+# gstablename=\'__GENE_SUMMARY_FILE__\'\n+startindex=3\n+# outputfile=\'__OUTPUT_FILE__\'\n+targetgenelist=c("ACIN1","ACTR8","AHCY","ACLY","AATF","AGBL5","AHCTF1","ABT1","ADIRF","ABCF1")\n+# samplelabel=sub(\'.\\\\w+.\\\\w+$\',\'\',colnames(gstable)[startindex]);\n+samplelabel=\'HL60.final,KBM7.final_vs_HL60.initial,KBM7.initial neg.\'\n+\n+\n+# You need to write some codes in front of this code:\n+# gstable=read.table(gstablename,header=T)\n+# pdf(file=outputfile,width=6,height=6)\n+\n+\n+# set up color using RColorBrewer\n+#library(RColorBrewer)\n+#colors <- brewer.pal(length(targetgenelist), "Set1")\n+\n+colors=c( "#E41A1C", "#377EB8", "#4DAF4A", "#984EA3", "#FF7F00", "#A65628", "#F781BF",\n+ "#999999", "#66C2A5", "#FC8D62", "#8DA0CB", "#E78AC3", "#A6D854", "#FFD92F", "#E5C494", "#B3B3B3", \n+ "#8DD3C7", "#FFFFB3", "#BEBADA", "#FB8072", "#80B1D3", "#FDB462", "#B3DE69", "#FCCDE5",\n+ "#D9D9D9", "#BC80BD", "#CCEBC5", "#FFED6F")\n+\n+######\n+# function definition\n+\n+plotrankedvalues<-function(val, tglist, ...){\n+ \n+ plot(val,log=\'y\',ylim=c(max(val),min(val)),type=\'l\',lwd=2, ...)\n+ if(length(tglist)>0){\n+ for(i in 1:length(tglist)){\n+ targetgene=tglist[i];\n+ tx=which(names(val)==targetgene);ty=val[targetgene];\n+ points(tx,ty,col=colors[(i %% length(colors)) ],cex=2,pch=20)\n+ # text(tx+50,ty,targetgene,col=colors[i])\n+ }\n+ legend(\'topright\',tglist,pch=20,pt.cex = 2,cex=1,col=colors)\n+ }\n+}\n+\n+\n+\n+plotrandvalues<-function(val,targetgenelist, ...){\n+ # choose the one with the best distance distribution\n+ \n+ mindiffvalue=0;\n+ randval=val;\n+ for(i in 1:20){\n+ randval0=sample(val)\n+ vindex=sort(which(names(randval0) %in% targetgenelist))\n+ if(max(vindex)>0.9*length(val)){\n+ # print(\'pass...\')\n+ next;\n+ }\n+ mindiffind=min(diff(vindex));\n+ if (mindiffind > mindiffvalue){\n+ mindiffvalue=mindiffind;\n+ randval=randval0;\n+ # print(paste(\'Diff: \',mindiffvalue))\n+ }\n+ }\n+ plot(randval,log=\'y\',ylim=c(max(randval),min(randval)),pch=20,col=\'grey\', ...)\n+ \n+ if(length(targetgenelist)>0){\n+ for(i in 1:length(targetgenelist)){\n+ targetgene=targetgenelist[i];\n+ tx=which(names(randval)==targetgene);ty=randval[targetgene];\n+ points(tx,ty,col=colors[(i %% length(colors)) ],cex=2,pch=20)\n+ text(tx+50,ty,targetgene,col=colors[i])\n+ }\n+ }\n+ \n+}\n+\n+\n+\n+\n+# set.seed(1235)\n+\n+\n+\n+pvec=gstable[,startindex]\n+names(pvec)=gstable[,\'id\']\n+pvec=sort(pvec);\n+\n+plotrankedvalues(pvec,targetgenelist,xlab=\'Genes\',ylab=\'RRA score\',main=paste(\'Distribution of RRA scores in \\\\n\',samplelabel))\n+\n+# plotrandvalues(pvec,targetgenelist,xlab=\'Genes\',ylab=\'RRA score\',main=paste(\'Distribution of RRA scores in \\\\n\',samplelabel))\n+\n+\n+pvec=gstable[,startindex+1]\n+names(pvec)=gstable[,\'id\']\n+pvec=sort(pvec);\n+\n+plotrankedvalues(pvec,targetgenelist,xlab=\'Genes\',ylab=\'p value\',main=paste(\'Distribution of p values in \\\\n\',samplelabel))\n+\n+# plotrandvalues(pvec,targetgenelist,xlab=\'Genes\',ylab=\'p value\',main=paste(\'Distribution of p values in \\\\n\',samplelabel))\n+\n+\n+\n+# you need to write after this code:\n+# dev.off()\n+\n+\n+\n+\n+\n+\n+# parameters\n+# Do not modify the variables beginning with "__"\n+targetmat=list(c(561.4907165816957,824.0396348113272,428.37415340969943,579.047491896501),c(3424.79939695118,3818.2871009576584,1992.3498917052,690.0506672205338),c(846.6456878299913,985.6508562937211,335.0024675413113,415.97581680707134),c(2432.636481525409,2122.257249136931,1067.465489792653,155.6333179800872),c(1308.1851773762019,2186.1913587343615,1482.5909580453515,997.3120339679854),c(405.68439208520414,268.16807081144486,170.34023773287015,109.85881269182627),c(640.8637498157573,559.4234589775174,711.6436598617687,632.2603542941043),c(946.5969148654764,470.6260845366416,663.0651476194316,45'..b'2554626,591.3905137762326,1061.787481868224,887.4532212761591),c(1655.0747300287676,943.0281165621008,1069.358159100796,2038.1098479598186),c(626.1650399575977,884.4218494311227,517.3296108924205,858.2719741548927),c(680.5502664327881,747.673892792174,533.1018551269456,1016.194017399393),c(662.9118146029966,777.8650001020718,864.9498738213518,787.3214909580882),c(880.4527205037583,621.5816210861304,671.8976043907657,1040.7978139918332),c(94.07174309222125,447.5387671820139,711.6436598617687,927.5059134033875),c(399.80490814194036,806.280159923152,1147.58849050404,1059.1076161071376),c(698.1887182625796,531.0082991564371,504.0809257354195,347.8862401907832))\n+targetgene="ACSS2"\n+collabel=c("HL60.initial","KBM7.initial","HL60.final","KBM7.final")\n+\n+# set up color using RColorBrewer\n+#library(RColorBrewer)\n+#colors <- brewer.pal(length(targetgenelist), "Set1")\n+\n+colors=c( "#E41A1C", "#377EB8", "#4DAF4A", "#984EA3", "#FF7F00", "#A65628", "#F781BF",\n+ "#999999", "#66C2A5", "#FC8D62", "#8DA0CB", "#E78AC3", "#A6D854", "#FFD92F", "#E5C494", "#B3B3B3", \n+ "#8DD3C7", "#FFFFB3", "#BEBADA", "#FB8072", "#80B1D3", "#FDB462", "#B3DE69", "#FCCDE5",\n+ "#D9D9D9", "#BC80BD", "#CCEBC5", "#FFED6F")\n+\n+\n+## code\n+\n+targetmatvec=unlist(targetmat)+1\n+yrange=range(targetmatvec[targetmatvec>0]);\n+# yrange[1]=1; # set the minimum value to 1\n+for(i in 1:length(targetmat)){\n+ vali=targetmat[[i]]+1;\n+ if(i==1){\n+ plot(1:length(vali),vali,type=\'b\',las=1,pch=20,main=paste(\'sgRNAs in\',targetgene),ylab=\'Read counts\',xlab=\'Samples\',xlim=c(0.7,length(vali)+0.3),ylim = yrange,col=colors[(i %% length(colors))],xaxt=\'n\',log=\'y\')\n+ axis(1,at=1:length(vali),labels=(collabel),las=2)\n+ # lines(0:100,rep(1,101),col=\'black\');\n+ }else{\n+ lines(1:length(vali),vali,type=\'b\',pch=20,col=colors[(i %% length(colors))])\n+ }\n+}\n+\n+\n+\n+\n+# parameters\n+# Do not modify the variables beginning with "__"\n+targetmat=list(c(408.62413405683606,523.9045092011671,483.89245311522745,701.494293542599),c(1805.0015705819953,1434.9655709645526,1712.2348341000356,2152.546111180471),c(3017.64513388016,2642.609863360463,1834.6274493599499,3573.2723190648703),c(1649.1952460855039,783.1928425685244,773.4708572611067,1332.0381038883936),c(959.82575373782,1397.6706736993847,1429.5962174173474,2811.126806015325),c(495.34652221997754,301.9110730989776,336.89513684945433,555.015876620164),c(1491.9190506031964,1331.9606166131366,2087.614246881731,1983.1804416139055),c(429.2023278582595,889.7496918975753,567.8007924429005,1132.9190058844583),c(427.7324568724435,573.6310388880576,655.4944703868597,899.4690289143276),c(690.8393633334998,767.2093151691668,1040.33722970927,993.3067647552625))\n+targetgene="ADNP"\n+collabel=c("HL60.initial","KBM7.initial","HL60.final","KBM7.final")\n+\n+# set up color using RColorBrewer\n+#library(RColorBrewer)\n+#colors <- brewer.pal(length(targetgenelist), "Set1")\n+\n+colors=c( "#E41A1C", "#377EB8", "#4DAF4A", "#984EA3", "#FF7F00", "#A65628", "#F781BF",\n+ "#999999", "#66C2A5", "#FC8D62", "#8DA0CB", "#E78AC3", "#A6D854", "#FFD92F", "#E5C494", "#B3B3B3", \n+ "#8DD3C7", "#FFFFB3", "#BEBADA", "#FB8072", "#80B1D3", "#FDB462", "#B3DE69", "#FCCDE5",\n+ "#D9D9D9", "#BC80BD", "#CCEBC5", "#FFED6F")\n+\n+\n+## code\n+\n+targetmatvec=unlist(targetmat)+1\n+yrange=range(targetmatvec[targetmatvec>0]);\n+# yrange[1]=1; # set the minimum value to 1\n+for(i in 1:length(targetmat)){\n+ vali=targetmat[[i]]+1;\n+ if(i==1){\n+ plot(1:length(vali),vali,type=\'b\',las=1,pch=20,main=paste(\'sgRNAs in\',targetgene),ylab=\'Read counts\',xlab=\'Samples\',xlim=c(0.7,length(vali)+0.3),ylim = yrange,col=colors[(i %% length(colors))],xaxt=\'n\',log=\'y\')\n+ axis(1,at=1:length(vali),labels=(collabel),las=2)\n+ # lines(0:100,rep(1,101),col=\'black\');\n+ }else{\n+ lines(1:length(vali),vali,type=\'b\',pch=20,col=colors[(i %% length(colors))])\n+ }\n+}\n+\n+\n+\n+dev.off()\n+Sweave("output_summary.Rnw");\n+library(tools);\n+\n+texi2dvi("output_summary.tex",pdf=TRUE);\n+\n' |
b |
diff -r 000000000000 -r b8da4d41aa1d test-data/out.test.gene_summary.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/out.test.gene_summary.txt Wed Feb 14 06:42:55 2018 -0500 |
b |
b'@@ -0,0 +1,101 @@\n+id\tnum\tneg|score\tneg|p-value\tneg|fdr\tneg|rank\tneg|goodsgrna\tneg|lfc\tpos|score\tpos|p-value\tpos|fdr\tpos|rank\tpos|goodsgrna\tpos|lfc\n+ACIN1\t10\t5.4294e-05\t0.00039421\t0.03518\t1\t9\t-0.8802\t0.98898\t0.98938\t0.994616\t98\t0\t-0.8802\n+ACTR8\t10\t0.00010064\t0.00070359\t0.03518\t2\t8\t-0.8638\t0.99479\t0.99462\t0.994616\t100\t0\t-0.8638\n+AHCY\t10\t0.00022009\t0.0015619\t0.051023\t3\t6\t-0.56069\t0.12721\t0.33167\t0.925081\t32\t2\t-0.56069\n+ACLY\t10\t0.00028907\t0.0020409\t0.051023\t4\t7\t-0.80032\t0.99461\t0.99442\t0.994616\t99\t0\t-0.80032\n+AATF\t10\t0.0015332\t0.0091467\t0.161427\t5\t8\t-0.70265\t0.9788\t0.98038\t0.994616\t96\t1\t-0.70265\n+AGBL5\t10\t0.0016215\t0.0096856\t0.161427\t6\t8\t-0.35789\t0.95384\t0.96824\t0.994616\t94\t1\t-0.35789\n+AHCTF1\t10\t0.0024367\t0.013897\t0.161843\t7\t5\t-0.23527\t0.73207\t0.88328\t0.994616\t85\t2\t-0.23527\n+ABT1\t10\t0.002511\t0.014316\t0.161843\t8\t7\t-0.58957\t0.81143\t0.91158\t0.994616\t87\t1\t-0.58957\n+ADIRF\t10\t0.0025672\t0.014566\t0.161843\t9\t7\t-0.38313\t0.51897\t0.75574\t0.994616\t71\t2\t-0.38313\n+ABCF1\t10\t0.0056079\t0.028159\t0.281587\t10\t5\t-0.082059\t0.2082\t0.46003\t0.925081\t49\t4\t-0.082059\n+ACTL6A\t10\t0.0081355\t0.038738\t0.352159\t11\t6\t-0.34362\t0.62569\t0.81868\t0.994616\t77\t2\t-0.34362\n+ACHE\t10\t0.011853\t0.053428\t0.433786\t12\t5\t-0.12062\t0.84881\t0.92691\t0.994616\t89\t2\t-0.12062\n+ADRB1\t10\t0.012576\t0.056392\t0.433786\t13\t3\t0.11433\t0.21006\t0.46255\t0.925081\t50\t5\t0.11433\n+ACTR3\t10\t0.014694\t0.064815\t0.462967\t14\t5\t-0.33481\t0.7562\t0.89168\t0.994616\t86\t1\t-0.33481\n+ACAD9\t10\t0.017938\t0.07741\t0.516068\t15\t5\t-0.31684\t0.014914\t0.066023\t0.551771\t11\t3\t-0.31684\n+ACTR1A\t10\t0.034608\t0.12629\t0.761624\t16\t5\t-0.022985\t0.10323\t0.29124\t0.925081\t29\t4\t-0.022985\n+ACP1\t10\t0.035849\t0.12948\t0.761624\t17\t6\t-0.18317\t0.98651\t0.98676\t0.994616\t97\t0\t-0.18317\n+ACTRT3\t10\t0.048507\t0.16474\t0.915197\t18\t5\t-0.42641\t0.17889\t0.41551\t0.925081\t41\t3\t-0.42641\n+AGPAT5\t10\t0.067777\t0.21474\t0.999686\t19\t5\t-0.18272\t0.83413\t0.92091\t0.994616\t88\t2\t-0.18272\n+AGPAT3\t10\t0.076532\t0.23376\t0.999686\t20\t5\t-0.26457\t0.48565\t0.73713\t0.994616\t68\t3\t-0.26457\n+ACO2\t10\t0.078341\t0.23737\t0.999686\t21\t3\t0.16994\t0.4585\t0.7169\t0.994616\t64\t4\t0.16994\n+AGL\t10\t0.10162\t0.28017\t0.999686\t22\t5\t-0.089501\t0.17049\t0.4023\t0.925081\t40\t4\t-0.089501\n+ABL1\t10\t0.10476\t0.28603\t0.999686\t23\t5\t-0.21329\t0.69153\t0.85923\t0.994616\t81\t2\t-0.21329\n+ACTL7A\t10\t0.10642\t0.28885\t0.999686\t24\t3\t-0.049861\t0.37001\t0.63545\t0.994616\t60\t4\t-0.049861\n+ADRM1\t10\t0.11044\t0.29611\t0.999686\t25\t5\t-0.21536\t0.92156\t0.95934\t0.994616\t93\t1\t-0.21536\n+AAAS\t10\t0.13331\t0.33635\t0.999686\t26\t5\t-0.29594\t0.8856\t0.94423\t0.994616\t92\t2\t-0.29594\n+ACVR1B\t10\t0.15016\t0.36584\t0.999686\t27\t5\t-0.13523\t0.46763\t0.72484\t0.994616\t66\t3\t-0.13523\n+ACBD6\t10\t0.15512\t0.37399\t0.999686\t28\t5\t-0.10807\t0.10027\t0.28586\t0.925081\t28\t4\t-0.10807\n+AFF3\t10\t0.15611\t0.37585\t0.999686\t29\t5\t-0.23592\t0.48266\t0.73528\t0.994616\t67\t3\t-0.23592\n+AAK1\t10\t0.16107\t0.38447\t0.999686\t30\t3\t0.067339\t0.020241\t0.085684\t0.648311\t13\t3\t0.067339\n+ABI1\t10\t0.16609\t0.39238\t0.999686\t31\t4\t-0.23736\t0.51898\t0.75574\t0.994616\t72\t2\t-0.23736\n+ADPRHL2\t10\t0.17117\t0.40059\t0.999686\t32\t3\t-0.0055285\t0.44948\t0.70813\t0.994616\t63\t3\t-0.0055285\n+AEN\t10\t0.1767\t0.40971\t0.999686\t33\t4\t-0.16395\t0.57302\t0.78776\t0.994616\t75\t1\t-0.16395\n+ACRC\t10\t0.17897\t0.4133\t0.999686\t34\t2\t0.11912\t0.00017229\t0.0012026\t0.120259\t1\t4\t0.11912\n+ADAM10\t10\t0.18631\t0.42512\t0.999686\t35\t5\t-0.1958\t0.68077\t0.85283\t0.994616\t80\t2\t-0.1958\n+ADH5\t10\t0.18633\t0.42515\t0.999686\t36\t4\t0.031022\t0.1918\t0.43499\t0.925081\t44\t4\t0.031022\n+AFMID\t10\t0.19212\t0.434\t0.999686\t37\t5\t-0.26972\t0.87179\t0.93752\t0.994616\t90\t1\t-0.26972\n+ABCC1\t10\t0.21094\t0.45995\t0.999686\t38\t4\t-0.15482\t0.034488\t0.12876\t0.757397\t17\t2\t-0.15482\n+ADAP1\t10\t0.21183\t0.46089\t0.999686\t39\t2\t0.096871\t0.25461\t0.51972\t0.944937\t55\t2\t0.096871\n+ACSL6\t10\t0.22008\t0.4703\t0.999686\t40\t3\t0.051392\t0.20601\t0.45666\t0.925081\t48\t5\t0.051392\n+ABTB1\t10\t0.22588\t0.47636\t0.999686\t41\t4\t-0.083354\t0.25131\t0.51632\t0.944937\t54\t3\t-0.083354\n+ACVR2B\t10\t0.23534\t0.48652\t0.999686\t42\t4\t0.055993\t0.19801\t0.4447\t0.925081\t46\t5\t0.055993\n+ACTR5\t10\t0.23574\t0.48692\t0.999686\t43\t1\t0.25177\t0.13602\t0.34662\t0.925081\t35\t5\t0.25177\n+ACTL6B\t10\t0.24356\t0.4953\t0.999'..b'842\t0.65928\t0.999686\t55\t2\t0.17169\t0.12854\t0.33421\t0.925081\t33\t5\t0.17169\n+ACAT2\t10\t0.40932\t0.6698\t0.999686\t56\t4\t0.14699\t0.15687\t0.38081\t0.925081\t39\t5\t0.14699\n+ADK\t10\t0.40951\t0.67\t0.999686\t57\t2\t0.26565\t0.0059541\t0.030474\t0.507901\t6\t5\t0.26565\n+ACAD11\t10\t0.42024\t0.68005\t0.999686\t58\t4\t-0.14423\t0.95975\t0.97042\t0.994616\t95\t1\t-0.14423\n+AGAP2\t10\t0.46176\t0.70849\t0.999686\t59\t2\t-0.056853\t0.29633\t0.56235\t0.986579\t57\t3\t-0.056853\n+AFF1\t10\t0.47065\t0.71388\t0.999686\t60\t3\t0.053306\t0.57765\t0.79016\t0.994616\t76\t2\t0.053306\n+AEBP2\t10\t0.48698\t0.72413\t0.999686\t61\t2\t0.012265\t0.52118\t0.75696\t0.994616\t73\t3\t0.012265\n+ABCB8\t10\t0.50647\t0.73644\t0.999686\t62\t3\t0.33882\t0.046462\t0.16162\t0.808109\t20\t6\t0.33882\n+ADRB3\t10\t0.51898\t0.7441\t0.999686\t63\t2\t0.19518\t0.038271\t0.13897\t0.772039\t18\t4\t0.19518\n+ADAR\t10\t0.52118\t0.74547\t0.999686\t64\t3\t-0.16099\t0.48698\t0.7378\t0.994616\t69\t3\t-0.16099\n+ACD\t10\t0.52413\t0.74766\t0.999686\t65\t3\t-0.029641\t0.19552\t0.44071\t0.925081\t45\t2\t-0.029641\n+AHNAK\t10\t0.5343\t0.75419\t0.999686\t66\t1\t0.37532\t0.061763\t0.20069\t0.850611\t23\t6\t0.37532\n+AFTPH\t10\t0.54301\t0.75984\t0.999686\t67\t2\t-0.077488\t0.37895\t0.64371\t0.994616\t61\t4\t-0.077488\n+AHNAK2\t10\t0.54917\t0.76409\t0.999686\t68\t2\t0.33608\t0.025649\t0.10417\t0.651042\t16\t6\t0.33608\n+ADRA1B\t10\t0.56639\t0.77483\t0.999686\t69\t2\t0.01884\t0.11831\t0.31693\t0.925081\t30\t3\t0.01884\n+ADCK4\t10\t0.57302\t0.77943\t0.999686\t70\t1\t0.33858\t0.0026444\t0.014995\t0.499834\t3\t8\t0.33858\n+ADCK5\t10\t0.58588\t0.78762\t0.999686\t71\t2\t0.046549\t0.025048\t0.10262\t0.651042\t15\t4\t0.046549\n+ADH7\t10\t0.58676\t0.78818\t0.999686\t72\t3\t-0.090919\t0.87513\t0.93916\t0.994616\t91\t2\t-0.090919\n+ADRBK1\t10\t0.59228\t0.7922\t0.999686\t73\t3\t0.36204\t0.0048237\t0.025634\t0.507901\t5\t7\t0.36204\n+ADRA1A\t10\t0.59776\t0.79555\t0.999686\t74\t3\t-0.066085\t0.051892\t0.17533\t0.832539\t21\t3\t-0.066085\n+AHRR\t9\t0.60697\t0.79866\t0.999686\t75\t2\t-0.049891\t0.0044955\t0.023119\t0.507901\t4\t3\t-0.049891\n+AFF2\t10\t0.62328\t0.81222\t0.999686\t76\t2\t0.2294\t0.24493\t0.5098\t0.944937\t53\t4\t0.2294\n+AATK\t10\t0.63527\t0.82015\t0.999686\t77\t2\t0.27488\t0.054954\t0.18316\t0.832539\t22\t6\t0.27488\n+AFF4\t10\t0.6797\t0.85053\t0.999686\t78\t2\t0.30669\t0.071064\t0.22367\t0.894671\t25\t6\t0.30669\n+A1CF\t10\t0.69378\t0.85821\t0.999686\t79\t2\t0.0064333\t0.14158\t0.35569\t0.925081\t37\t4\t0.0064333\n+ADD1\t10\t0.74472\t0.87754\t0.999686\t80\t2\t0.11567\t0.34577\t0.61089\t0.994616\t58\t4\t0.11567\n+ADAD1\t10\t0.75052\t0.87973\t0.999686\t81\t2\t-0.0043567\t0.50142\t0.74596\t0.994616\t70\t2\t-0.0043567\n+ACTN4\t10\t0.80918\t0.90438\t0.999686\t82\t2\t0.2223\t0.021612\t0.090763\t0.648311\t14\t7\t0.2223\n+ADARB1\t10\t0.81584\t0.90757\t0.999686\t83\t1\t0.11123\t0.13975\t0.3527\t0.925081\t36\t4\t0.11123\n+ACSS2\t10\t0.82229\t0.91034\t0.999686\t84\t1\t0.19323\t0.013868\t0.061861\t0.551771\t9\t4\t0.19323\n+ADRB2\t10\t0.83749\t0.91809\t0.999686\t85\t2\t-0.009076\t0.70203\t0.86569\t0.994616\t82\t3\t-0.009076\n+AFAP1L2\t10\t0.85952\t0.92884\t0.999686\t86\t2\t0.23074\t0.21621\t0.47179\t0.925081\t51\t5\t0.23074\n+ACTN1\t10\t0.86536\t0.93211\t0.999686\t87\t2\t0.12145\t0.28536\t0.55189\t0.98552\t56\t3\t0.12145\n+ADARB2\t10\t0.87336\t0.93654\t0.999686\t88\t1\t0.36865\t0.0098559\t0.046302\t0.551771\t8\t6\t0.36865\n+ADCK2\t10\t0.88046\t0.94066\t0.999686\t89\t2\t-0.0071793\t0.3792\t0.64399\t0.994616\t62\t4\t-0.0071793\n+ABL2\t10\t0.92156\t0.95311\t0.999686\t90\t1\t0.15642\t0.53574\t0.76569\t0.994616\t74\t3\t0.15642\n+ADRBK2\t10\t0.9506\t0.96302\t0.999686\t91\t1\t0.2293\t0.2\t0.44756\t0.925081\t47\t5\t0.2293\n+ADCK1\t10\t0.96245\t0.96866\t0.999686\t92\t1\t0.31253\t0.0081355\t0.039596\t0.551771\t7\t6\t0.31253\n+ADNP2\t10\t0.96448\t0.96988\t0.999686\t93\t1\t0.11441\t0.14636\t0.36385\t0.925081\t38\t5\t0.11441\n+AGTPBP1\t10\t0.97247\t0.97465\t0.999686\t94\t1\t0.018825\t0.17891\t0.41555\t0.925081\t42\t4\t0.018825\n+ACVR1\t10\t0.97475\t0.97633\t0.999686\t95\t1\t0.22596\t0.09861\t0.28311\t0.925081\t27\t5\t0.22596\n+ADCK3\t10\t0.9877\t0.98797\t0.999686\t96\t0\t0.34147\t0.014963\t0.066213\t0.551771\t12\t6\t0.34147\n+AGAP3\t10\t0.99117\t0.99131\t0.999686\t97\t0\t0.36057\t0.0012992\t0.0079691\t0.398453\t2\t8\t0.36057\n+ADNP\t10\t0.99212\t0.99227\t0.999686\t98\t0\t0.35587\t0.014351\t0.063787\t0.551771\t10\t7\t0.35587\n+AES\t10\t0.9959\t0.99575\t0.999686\t99\t0\t0.21432\t0.24171\t0.50676\t0.944937\t52\t4\t0.21432\n+ACVR1C\t10\t0.99964\t0.99969\t0.999686\t100\t0\t0.29763\t0.042729\t0.15142\t0.796959\t19\t6\t0.29763\n' |
b |
diff -r 000000000000 -r b8da4d41aa1d test-data/out.test.log.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/out.test.log.txt Wed Feb 14 06:42:55 2018 -0500 |
b |
b'@@ -0,0 +1,112 @@\n+INFO @ Mon, 12 Feb 2018 03:40:01: Parameters: /home/maria/miniconda3/envs/mulled-v1-b1e6893952d2d7e4d51a13d040adcd7bea052bfdc57a24d59056014db875e749/bin/mageck test -k /tmp/tmp0xS0Jl/files/000/dataset_21.dat -t HL60.final,KBM7.final -c HL60.initial,KBM7.initial -n output --normcounts-to-file --pdf-report --norm-method median --gene-test-fdr-threshold 0.25 --adjust-method fdr --sort-criteria neg --remove-zero none --gene-lfc-method median \n+INFO @ Mon, 12 Feb 2018 03:40:01: Welcome to MAGeCK v0.5.7. Command: test \n+INFO @ Mon, 12 Feb 2018 03:40:01: Loading count table from /tmp/tmp0xS0Jl/files/000/dataset_21.dat \n+INFO @ Mon, 12 Feb 2018 03:40:01: Processing 1 lines.. \n+DEBUG @ Mon, 12 Feb 2018 03:40:01: Parsing error in line 1 (usually the header line). Skip this line. \n+INFO @ Mon, 12 Feb 2018 03:40:01: Loaded 999 records. \n+INFO @ Mon, 12 Feb 2018 03:40:01: Loading R template file: /home/maria/miniconda3/envs/mulled-v1-b1e6893952d2d7e4d51a13d040adcd7bea052bfdc57a24d59056014db875e749/lib/python3.6/site-packages/mageck/plot_template.RTemplate. \n+INFO @ Mon, 12 Feb 2018 03:40:01: Loading R template file: /home/maria/miniconda3/envs/mulled-v1-b1e6893952d2d7e4d51a13d040adcd7bea052bfdc57a24d59056014db875e749/lib/python3.6/site-packages/mageck/plot_template_indvgene.RTemplate. \n+INFO @ Mon, 12 Feb 2018 03:40:01: Loading Rnw template file: /home/maria/miniconda3/envs/mulled-v1-b1e6893952d2d7e4d51a13d040adcd7bea052bfdc57a24d59056014db875e749/lib/python3.6/site-packages/mageck/plot_template.Rnw. \n+DEBUG @ Mon, 12 Feb 2018 03:40:01: Setting up the visualization module... \n+DEBUG @ Mon, 12 Feb 2018 03:40:01: Given sample labels: HL60.final,KBM7.final \n+DEBUG @ Mon, 12 Feb 2018 03:40:01: Converted index: 2 3 \n+INFO @ Mon, 12 Feb 2018 03:40:01: Treatment samples:HL60.final,KBM7.final \n+INFO @ Mon, 12 Feb 2018 03:40:01: Treatment sample index:2,3 \n+DEBUG @ Mon, 12 Feb 2018 03:40:01: Given sample labels: HL60.initial,KBM7.initial \n+DEBUG @ Mon, 12 Feb 2018 03:40:01: Converted index: 0 1 \n+INFO @ Mon, 12 Feb 2018 03:40:01: Control samples:HL60.initial,KBM7.initial \n+INFO @ Mon, 12 Feb 2018 03:40:01: Control sample index:0,1 \n+DEBUG @ Mon, 12 Feb 2018 03:40:01: Initial (total) size factor: 1.6666455325878438 2.027372749328462 0.7198064117880387 0.6589869375844738 \n+DEBUG @ Mon, 12 Feb 2018 03:40:01: Median factor: 1.469870985815957 1.7759474888175155 0.6308897693810006 0.5721813161032618 \n+INFO @ Mon, 12 Feb 2018 03:40:01: Final size factor: 1.469870985815957 1.7759474888175155 0.6308897693810006 0.5721813161032618 \n+INFO @ Mon, 12 Feb 2018 03:40:01: Writing normalized read counts to output.normalized.txt \n+DEBUG @ Mon, 12 Feb 2018 03:40:01: Adjusted model: 1.1175084644498339\t3.4299551007579927 \n+INFO @ Mon, 12 Feb 2018 03:40:01: Raw variance calculation: 0.5 for control, 0.5 for treatment \n+INFO @ Mon, 12 Feb 2018 03:40:01: Adjusted variance calculation: 0.3333333333333333 for raw variance, 0.6666666666666667 for modeling \n+INFO @ Mon, 12 Feb 2018 03:40:01: Use qnorm to reversely calculate sgRNA scores ... \n+DEBUG @ Mon, 12 Feb 2018 03:40:01: lower test FDR cutoff: 0.3283283283283283 \n+DEBUG @ Mon, 12 Feb 2018 03:40:01: higher test FDR cutoff: 0.34534534534534533 \n+INFO @ Mon, 12 Feb 2018 03:40:01: Running command: RRA -i output.plow.txt -o output.gene.low.txt -p 0.3283283283283283 --skip-gene NA --skip-gene na \n+INFO @ Mon, 12 Feb 2018 03:40:02: Command message: \n+INFO @ Mon, 12 Feb 2018 03:40:02: Welcome to RRA v 0.5.7. \n+INFO @ Mon, 12 Feb 2018 03:40:02: Skipping gene NA for permutation ... \n+INFO @ Mon, 12 Feb 2018 03:40:02: Skipping gene na for permutation ... \n+INFO @ Mon, 12 Feb 2018 03:40:02: Reading input file... \n+INFO @ Mon, 12 Feb 2018 03:40:02: Summary: 999 sgRNAs, 100 genes, 1 lists; skipped sgRNAs:0 \n+INFO @ Mon, 12 Feb 2018 03:40:02: Computing lo-values for each group... \n+INFO @ Mon, 12 Feb 2018 03:40:02: Computing false discovery rate... \n+INFO @ Mon, 12 Feb 201'..b'nt: 100 \n+INFO @ Mon, 12 Feb 2018 03:40:03: Saving to output file... \n+INFO @ Mon, 12 Feb 2018 03:40:03: RRA completed. \n+INFO @ Mon, 12 Feb 2018 03:40:03: \n+INFO @ Mon, 12 Feb 2018 03:40:03: End command message. \n+DEBUG @ Mon, 12 Feb 2018 03:40:03: Sorting the merged items by negative selection... \n+INFO @ Mon, 12 Feb 2018 03:40:03: Loading top 10 genes from output.gene.low.txt: ACIN1,ACTR8,AHCY,ACLY,AATF,AGBL5,AHCTF1,ABT1,ADIRF,ABCF1 \n+DEBUG @ Mon, 12 Feb 2018 03:40:03: Column index:3 \n+INFO @ Mon, 12 Feb 2018 03:40:03: Loading top 10 genes from output.gene.high.txt: ACRC,AGAP3,ADCK4,AHRR,ADRBK1,ADK,ADCK1,ADARB2,ACSS2,ADNP \n+DEBUG @ Mon, 12 Feb 2018 03:40:03: Column index:9 \n+INFO @ Mon, 12 Feb 2018 03:40:03: Running command: rm output.plow.txt \n+INFO @ Mon, 12 Feb 2018 03:40:03: Running command: rm output.phigh.txt \n+INFO @ Mon, 12 Feb 2018 03:40:03: Running command: rm output.gene.low.txt \n+INFO @ Mon, 12 Feb 2018 03:40:03: Running command: rm output.gene.high.txt \n+INFO @ Mon, 12 Feb 2018 03:40:03: Running command: cd ./; Rscript output.R \n+INFO @ Mon, 12 Feb 2018 03:40:04: Command message: \n+INFO @ Mon, 12 Feb 2018 03:40:04: null device \n+INFO @ Mon, 12 Feb 2018 03:40:04: 1 \n+INFO @ Mon, 12 Feb 2018 03:40:04: Writing to file output_summary.tex \n+INFO @ Mon, 12 Feb 2018 03:40:04: Processing code chunks with options ... \n+INFO @ Mon, 12 Feb 2018 03:40:04: 1 : keep.source term verbatim (label = funcdef, output_summary.Rnw:27) \n+INFO @ Mon, 12 Feb 2018 03:40:04: 2 : keep.source term tex (label = tab1, output_summary.Rnw:37) \n+INFO @ Mon, 12 Feb 2018 03:40:04: 3 : keep.source term verbatim (output_summary.Rnw:77) \n+INFO @ Mon, 12 Feb 2018 03:40:04: 4 : keep.source term verbatim pdf (output_summary.Rnw:83) \n+INFO @ Mon, 12 Feb 2018 03:40:04: 5 : keep.source term verbatim pdf (output_summary.Rnw:201) \n+INFO @ Mon, 12 Feb 2018 03:40:04: 6 : keep.source term verbatim pdf (output_summary.Rnw:345) \n+INFO @ Mon, 12 Feb 2018 03:40:04: 7 : keep.source term verbatim pdf (output_summary.Rnw:489) \n+INFO @ Mon, 12 Feb 2018 03:40:04: 8 : keep.source term verbatim (output_summary.Rnw:567) \n+INFO @ Mon, 12 Feb 2018 03:40:04: 9 : keep.source term verbatim pdf (output_summary.Rnw:573) \n+INFO @ Mon, 12 Feb 2018 03:40:04: 10 : keep.source term verbatim pdf (output_summary.Rnw:691) \n+INFO @ Mon, 12 Feb 2018 03:40:04: 11 : keep.source term verbatim pdf (output_summary.Rnw:835) \n+INFO @ Mon, 12 Feb 2018 03:40:04: 12 : keep.source term verbatim pdf (output_summary.Rnw:979) \n+INFO @ Mon, 12 Feb 2018 03:40:04: \n+INFO @ Mon, 12 Feb 2018 03:40:04: You can now run (pdf)latex on \xe2\x80\x98output_summary.tex\xe2\x80\x99 \n+INFO @ Mon, 12 Feb 2018 03:40:04: Error in texi2dvi("output_summary.tex", pdf = TRUE) : \n+INFO @ Mon, 12 Feb 2018 03:40:04: pdflatex is not available \n+INFO @ Mon, 12 Feb 2018 03:40:04: Execution halted \n+INFO @ Mon, 12 Feb 2018 03:40:04: \n+INFO @ Mon, 12 Feb 2018 03:40:04: End command message. \n+INFO @ Mon, 12 Feb 2018 03:40:04: Running command: cd ./; rm -rf output_summary-*.pdf \n+INFO @ Mon, 12 Feb 2018 03:40:04: Command message: \n+INFO @ Mon, 12 Feb 2018 03:40:04: \n+INFO @ Mon, 12 Feb 2018 03:40:04: End command message. \n+INFO @ Mon, 12 Feb 2018 03:40:04: Running command: cd ./; rm -rf output_summary.aux \n+INFO @ Mon, 12 Feb 2018 03:40:04: Command message: \n+INFO @ Mon, 12 Feb 2018 03:40:04: \n+INFO @ Mon, 12 Feb 2018 03:40:04: End command message. \n+INFO @ Mon, 12 Feb 2018 03:40:04: Running command: cd ./; rm -rf output_summary.tex \n+INFO @ Mon, 12 Feb 2018 03:40:04: Command message: \n+INFO @ Mon, 12 Feb 2018 03:40:04: \n+INFO @ Mon, 12 Feb 2018 03:40:04: End command message. \n+INFO @ Mon, 12 Feb 2018 03:40:04: Running command: cd ./; rm -rf output_summary.toc \n+INFO @ Mon, 12 Feb 2018 03:40:04: Command message: \n+INFO @ Mon, 12 Feb 2018 03:40:04: \n+INFO @ Mon, 12 Feb 2018 03:40:04: End command message. \n' |
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diff -r 000000000000 -r b8da4d41aa1d test-data/out.test.normalized.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/out.test.normalized.txt Wed Feb 14 06:42:55 2018 -0500 |
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b'@@ -0,0 +1,1000 @@\n+sgRNA\tGene\tHL60.initial\tKBM7.initial\tHL60.final\tKBM7.final\n+A1CF_m52595977\tA1CF\t313.08251997879887\t486.6096119359992\t557.0756663634236\t100.13173031807082\n+A1CF_m52596017\tA1CF\t432.1420698298914\t731.6903653928164\t980.402701618075\t1081.994868751268\n+A1CF_m52596056\tA1CF\t618.8156850285179\t653.5486758848457\t357.0836094696463\t434.28561892237576\n+A1CF_m52603842\tA1CF\t402.7446501135722\t431.55523978265626\t198.0993875856342\t489.21502526828885\n+A1CF_m52603847\tA1CF\t0.0\t88.79737444087577\t91.47901656024509\t152.20023008346766\n+A1CF_p52595870\tA1CF\t915.7296241633413\t1035.3773859806115\t948.227323379644\t639.1265300873434\n+A1CF_p52595881\tA1CF\t714.3572991065552\t671.3081507730209\t1119.829340651276\t906.90738602367\n+A1CF_p52596023\tA1CF\t286.6248422341116\t761.8814727027142\t493.9866894253235\t442.86833866392465\n+A1CF_p52601638\tA1CF\t1093.584013447072\t815.1598973672396\t777.8870856467737\t1377.2404278605513\n+A1CF_p52603829\tA1CF\t111.71019492201273\t252.1845434120872\t209.4554034344922\t269.4973998846363\n+AAAS_m53714382\tAAAS\t1034.7891740144337\t1191.6607649965529\t504.0809257354195\t815.9305567632514\n+AAAS_m53715169\tAAAS\t956.8860117661881\t1113.5190754885823\t502.81914619665747\t966.9864242145125\n+AAAS_m53715176\tAAAS\t801.0796872696966\t158.05932650475887\t247.30878959735224\t379.92839389256585\n+AAAS_m53715212\tAAAS\t373.3472303972531\t603.8221461979552\t270.6517110644493\t424.5585365486203\n+AAAS_m53715238\tAAAS\t47.03587154611063\t239.7529109903646\t265.6045929094013\t44.05796133995116\n+AAAS_p53714367\tAAAS\t474.7683284185541\t582.510776332145\t411.34012963641237\t268.35303725242983\n+AAAS_p53714374\tAAAS\t430.6721988440754\t463.52229458137157\t540.0416425901366\t553.8715139879574\n+AAAS_p53714391\tAAAS\t415.9734889859159\t708.6030480381887\t601.8688399894746\t537.8504371370661\n+AAAS_p53714405\tAAAS\t1644.785633128056\t1520.2110504277932\t1395.5281698707734\t1553.4722732203559\n+AAAS_p53714441\tAAAS\t401.2747791277563\t580.7348288433276\t689.5625179334337\t474.33831104960404\n+AAK1_m69870049\tAAK1\t535.0330388370083\t825.8155823001447\t437.2066101810334\t1147.7957201031431\n+AAK1_m69870056\tAAK1\t933.3680759931327\t1255.5948745939834\t1409.4077447971554\t2011.2173261029654\n+AAK1_m69870103\tAAK1\t712.8874281207392\t802.728264945517\t576.6332492142345\t359.9020478289517\n+AAK1_m69870119\tAAK1\t595.2977492554626\t488.3855594248168\t508.4971541210865\t478.91576157843014\n+AAK1_m69870125\tAAK1\t402.7446501135722\t621.5816210861304\t1149.481159812183\t1202.1529451329532\n+AAK1_m69870131\tAAK1\t1108.2827233052317\t1355.0479339677643\t890.1854645965918\t1508.8421305643014\n+AAK1_m69870137\tAAK1\t1330.2332421634412\t522.1285617123496\t323.0155619230723\t456.6006902504029\n+AAK1_p69870063\tAAK1\t485.05742531926586\t296.5832306325251\t229.64387605468423\t651.7145190416153\n+AAK1_p69870070\tAAK1\t1456.6421469436134\t1275.1302969709761\t1129.292687191991\t962.4089736856864\n+AAK1_p69870105\tAAK1\t279.27548730503185\t289.479440677255\t581.6803673692825\t639.6987114034467\n+AATF_m35306444\tAATF\t659.9720726313648\t809.832054900787\t880.7221180558769\t802.1982051767731\n+AATF_m35306475\tAATF\t724.6463960072668\t1086.8798631563195\t695.2405258578626\t307.26136674745163\n+AATF_m35306482\tAATF\t836.3565909292796\t1289.3378768815162\t468.75109865008346\t177.94838930811443\n+AATF_m35306509\tAATF\t367.46774645398926\t571.85509139924\t300.30353022535627\t116.72498848506541\n+AATF_m35307516\tAATF\t518.8644579930328\t632.2373060190355\t627.7353205340956\t308.9779106957614\n+AATF_m35307525\tAATF\t405.68439208520414\t259.28833336735727\t324.27734146183434\t166.5047629860492\n+AATF_p35306410\tAATF\t2096.0360257735547\t1960.6460276545372\t1573.4390848362154\t629.9716290296913\n+AATF_p35306466\tAATF\t277.8056163192159\t435.1071347602913\t182.32714335110919\t0.0\n+AATF_p35307505\tAATF\t995.1026573974029\t477.7298744919117\t728.0467938656747\t275.21921304566894\n+AATF_p35307527\tAATF\t2185.6981559083283\t1482.9161531626255\t1741.8866532609427\t1862.4501839161173\n+AATK_m79102275\tAATK\t912.7898821917094\t772.5371576356192\t2881.904466532411\t634.5490795585174\n+AATK_m79102281\tAATK\t683.49000840442\t816.9358448560571\t1095.224639645417\t1516.85266898'..b'+AHCTF1_m247094617\tAHCTF1\t845.1758168441753\t660.6524658401157\t541.3034221288985\t640.8430740356532\n+AHCTF1_p247076572\tAHCTF1\t551.2016196809839\t740.570102836904\t1103.42620664737\t622.5332719203489\n+AHCTF1_p247079429\tAHCTF1\t601.1772331987264\t900.4053768304803\t735.6174710982467\t754.1349746240991\n+AHCY_m32883210\tAHCY\t301.3235520922712\t657.1005708624807\t228.38209651592223\t137.32351586478285\n+AHCY_m32883247\tAHCY\t1142.0897559789987\t1099.311495578042\t112.92926871919911\t100.70391163417409\n+AHCY_m32883267\tAHCY\t789.3207193831689\t671.3081507730209\t723.6305654800077\t588.7745742702564\n+AHCY_m32883301\tAHCY\t392.45555321286054\t412.0198174056636\t334.37157777193033\t213.99581222261992\n+AHCY_m32883330\tAHCY\t2009.3136376104133\t2235.917888421252\t2437.1271791188055\t1937.9781176417478\n+AHCY_p32883227\tAHCY\t1071.5359486598327\t406.69197493921104\t645.4002340767636\t349.602784139093\n+AHCY_p32883238\tAHCY\t61.7345814042702\t218.44154112455442\t614.4866353770946\t452.5954210376801\n+AHCY_p32883253\tAHCY\t651.152846716469\t879.0940069646701\t237.21455328725622\t18.88198343140764\n+AHCY_p32883304\tAHCY\t1625.6773103124485\t1410.1023061211074\t2146.286995434164\t1986.613529510525\n+AHCY_p32883309\tAHCY\t1053.8974968300413\t882.6459019423052\t106.6203710253891\t105.85354347910344\n+AHNAK2_m105423801\tAHNAK2\t1215.5833052697965\t1655.1830595779245\t1491.4234148166854\t1543.7451908466005\n+AHNAK2_m105423809\tAHNAK2\t404.2145210993882\t470.6260845366416\t489.5704610396565\t643.1317993000663\n+AHNAK2_m105423822\tAHNAK2\t316.0222619504308\t735.2422603704514\t709.7509905536257\t664.8746893119902\n+AHNAK2_m105423829\tAHNAK2\t737.8752348796105\t879.0940069646701\t561.4918947490905\t402.81564653669636\n+AHNAK2_m105423836\tAHNAK2\t293.97419716319143\t816.9358448560571\t1369.0307995567714\t1079.1339621707518\n+AHNAK2_m105444536\tAHNAK2\t382.16645631214885\t454.642557137284\t707.8583212454827\t608.8009203338706\n+AHNAK2_m105444541\tAHNAK2\t761.3931706526657\t664.2043608177509\t846.6540705093029\t909.1961112880831\n+AHNAK2_m105444555\tAHNAK2\t504.1657481348733\t277.0478082555324\t162.13867073091717\t738.1138977732078\n+AHNAK2_p105423957\tAHNAK2\t676.1406534753403\t552.3196690222474\t313.5522153823573\t654.0032443060283\n+AHNAK2_p105423983\tAHNAK2\t363.0581334965414\t388.9325000510359\t977.24825277117\t556.1602392523705\n+AHNAK_m62303488\tAHNAK\t399.80490814194036\t504.3690868241744\t781.0415344936788\t643.1317993000663\n+AHNAK_m62303507\tAHNAK\t116.11980787946061\t236.20101601272955\t292.7328529927843\t19.4541647475109\n+AHNAK_m62303523\tAHNAK\t179.32426026954676\t298.3591781213426\t150.15176511267813\t305.5448227991418\n+AHNAK_m62303528\tAHNAK\t266.04664843268824\t797.4004224790644\t677.5756123151947\t839.3899907234851\n+AHNAK_m62303548\tAHNAK\t868.6937526172306\t980.3230138272686\t721.1070064024836\t327.859894127169\n+AHNAK_m62303560\tAHNAK\t704.0682022058435\t959.0116439614584\t1027.71943432165\t1504.8368613515786\n+AHNAK_p62303470\tAHNAK\t379.22671434051693\t731.6903653928164\t881.3530078252578\t997.3120339679854\n+AHNAK_p62303477\tAHNAK\t655.5624596739169\t500.8171918465394\t554.5521072858995\t475.4826736818106\n+AHNAK_p62303510\tAHNAK\t473.2984574327382\t451.09066215964896\t598.0835013731886\t493.2202944810117\n+AHNAK_p62303551\tAHNAK\t51.4454845035585\t40.846792242802856\t117.34549710486611\t31.4699723856794\n+AHRR_m344006\tAHRR\t345.4196816667499\t163.38716897121142\t474.42910657451245\t481.2044868428432\n+AHRR_m344069\tAHRR\t415.9734889859159\t372.9489726516783\t212.6098522813972\t349.03060282298975\n+AHRR_m353878\tAHRR\t1.469870985815957\t83.46953197442323\t0.0\t62.9399447713588\n+AHRR_m353892\tAHRR\t351.29916561001374\t150.9555365494888\t288.9475143764983\t416.54799812317464\n+AHRR_m353898\tAHRR\t561.4907165816957\t170.49095892648148\t199.3611671243962\t411.97054759434855\n+AHRR_p344008\tAHRR\t251.34793857452865\t221.99343610218943\t1564.6066280648815\t1502.5481360871656\n+AHRR_p344030\tAHRR\t736.4053638937945\t893.3015868752103\t1114.782222496228\t459.46159683091923\n+AHRR_p344036\tAHRR\t338.07032673767014\t607.3740411755903\t378.5338616286004\t65.22867003577186\n+AHRR_p344042\tAHRR\t1230.2820151279561\t525.6804566899846\t837.1907239685878\t945.2435342025885\n' |
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diff -r 000000000000 -r b8da4d41aa1d test-data/out.test.pdf |
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Binary file test-data/out.test.pdf has changed |
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diff -r 000000000000 -r b8da4d41aa1d test-data/out.test.sgrna_summary.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/out.test.sgrna_summary.txt Wed Feb 14 06:42:55 2018 -0500 |
b |
b'@@ -0,0 +1,1000 @@\n+sgrna\tGene\tcontrol_count\ttreatment_count\tcontrol_mean\ttreat_mean\tLFC\tcontrol_var\tadj_var\tscore\tp.low\tp.high\tp.twosided\tFDR\thigh_in_treatment\n+AHRR_p344008\tAHRR\t251.35/221.99\t1564.6/1502.5\t236.67\t1533.6\t2.6908\t1178.2\t5085.6\t18.186\t1\t3.3319e-74\t6.6638e-74\t6.6571e-71\tTrue\n+ACAD9_m128598565\tACAD9\t739.35/925.27\t2528/2050.7\t832.31\t2289.3\t1.4586\t65590\t35597\t7.7226\t1\t5.7002e-15\t1.14e-14\t5.6945e-12\tTrue\n+ACTR8_m53916081\tACTR8\t1925.5/2054.8\t194.94/235.17\t1990.2\t215.06\t-3.2041\t4580.2\t54357\t7.6137\t1.3311e-14\t1\t2.6622e-14\t7.8608e-12\tFalse\n+ACRC_m70814198\tACRC\t76.433/90.573\t447.3/357.04\t83.503\t402.17\t2.2543\t2086.7\t1760.4\t7.5952\t1\t1.5737e-14\t3.1475e-14\t7.8608e-12\tTrue\n+ABCC1_p16101710\tABCC1\t52.915/26.639\t203.78/224.3\t39.777\t214.04\t2.3987\t277.85\t700.66\t6.5833\t1\t2.465e-11\t4.93e-11\t9.8502e-09\tTrue\n+ACTR8_m53916067\tACTR8\t1267/1156.1\t251.09/42.341\t1211.6\t146.72\t-3.0372\t13968\t31286\t6.021\t8.6646e-10\t1\t1.7329e-09\t2.3419e-07\tFalse\n+AAK1_m69870125\tAAK1\t402.74/621.58\t1149.5/1202.2\t512.16\t1175.8\t1.1974\t12666\t12223\t6.0027\t1\t9.7009e-10\t1.9402e-09\t2.3419e-07\tTrue\n+ADCK1_p78285331\tADCK1\t798.14/768.99\t1478.8/1756\t783.56\t1617.4\t1.0446\t19425\t19317\t5.9996\t1\t9.8882e-10\t1.9776e-09\t2.3419e-07\tTrue\n+AHCY_m32883247\tAHCY\t1142.1/1099.3\t112.93/100.7\t1120.7\t106.82\t-3.379\t494.86\t28685\t5.9891\t1.0549e-09\t1\t2.1098e-09\t2.3419e-07\tFalse\n+AHCTF1_m247070995\tAHCTF1\t1437.5/1095.8\t320.49/161.36\t1266.6\t240.92\t-2.3895\t35534\t33759\t5.5826\t1.1847e-08\t1\t2.3695e-08\t2.3671e-06\tFalse\n+AHCY_p32883309\tAHCY\t1053.9/882.65\t106.62/105.85\t968.27\t106.24\t-3.1761\t7331.9\t24378\t5.524\t1.6565e-08\t1\t3.3129e-08\t3.0087e-06\tFalse\n+ADCY1_p45614315\tADCY1\t2.9397/72.814\t210.72/187.1\t37.877\t198.91\t2.3624\t1360\t895.72\t5.3806\t1\t4.1374e-08\t8.2747e-08\t6.6138e-06\tTrue\n+ACTN4_p39138476\tACTN4\t449.78/475.95\t1056.7/977.86\t462.87\t1017.3\t1.1344\t1726.9\t10724\t5.3539\t1\t4.3033e-08\t8.6065e-08\t6.6138e-06\tTrue\n+ADAM12_m128076658\tADAM12\t768.74/767.21\t1432.1/1562.6\t767.98\t1497.4\t0.96239\t4258.6\t18836\t5.3145\t1\t5.3464e-08\t1.0693e-07\t7.63e-06\tTrue\n+ABT1_m26597388\tABT1\t1743.3/1980.2\t837.19/281.51\t1861.7\t559.35\t-1.733\t91226\t64053\t5.1459\t1.3309e-07\t1\t2.6618e-07\t1.7727e-05\tFalse\n+ACRC_p70814182\tACRC\t682.02/822.26\t1572.2/1333.8\t752.14\t1453\t0.949\t19128\t18646\t5.1324\t1\t1.4307e-07\t2.8614e-07\t1.7866e-05\tTrue\n+ACBD6_p180471256\tACBD6\t107.3/285.93\t553.29/687.76\t196.61\t620.53\t1.6531\t12498\t6924.5\t5.0942\t1\t1.7666e-07\t3.5333e-07\t2.0382e-05\tTrue\n+ACRC_p70811990\tACRC\t495.35/605.6\t1159.6/1617.6\t550.47\t1388.6\t1.3333\t55476\t27162\t5.0853\t1\t1.8362e-07\t3.6724e-07\t2.0382e-05\tTrue\n+ADRA1B_m159344001\tADRA1B\t329.25/309.01\t881.98/676.32\t319.13\t779.15\t1.2851\t10677\t8286.5\t5.0535\t1\t2.1699e-07\t4.3398e-07\t2.2818e-05\tTrue\n+AHCY_p32883238\tAHCY\t61.735/218.44\t614.49/452.6\t140.09\t533.54\t1.9217\t12691\t6123\t5.0282\t1\t2.5699e-07\t5.1398e-07\t2.5673e-05\tTrue\n+ACTR5_m37377141\tACTR5\t865.75/935.92\t1595.5/1695.4\t900.84\t1645.4\t0.86841\t3723.6\t22496\t4.9645\t1\t3.4447e-07\t6.8893e-07\t3.2774e-05\tTrue\n+ADARB2_m1779330\tADARB2\t135.23/118.99\t351.41/399.95\t127.11\t375.68\t1.556\t655.19\t2547.9\t4.9245\t1\t4.2536e-07\t8.5071e-07\t3.863e-05\tTrue\n+ADRB1_m115804012\tADRB1\t2166.6/1942.9\t1093.3/705.5\t2054.7\t899.42\t-1.191\t50114\t56324\t4.8681\t5.6346e-07\t1\t1.1269e-06\t4.8948e-05\tFalse\n+AHCTF1_m247070906\tAHCTF1\t1078.9/1609\t348.88/193.97\t1343.9\t271.43\t-2.3036\t76257\t48827\t4.8539\t6.0526e-07\t1\t1.2105e-06\t5.0388e-05\tFalse\n+ACIN1_m23538803\tACIN1\t2432.6/2122.3\t1067.5/155.63\t2277.4\t611.55\t-1.8952\t2.3194e+05\t1.1942e+05\t4.8207\t7.1537e-07\t1\t1.4307e-06\t5.7173e-05\tFalse\n+ACIN1_m23538698\tACIN1\t3424.8/3818.3\t1992.3/690.05\t3621.5\t1341.2\t-1.4324\t4.627e+05\t2.2481e+05\t4.8094\t7.5677e-07\t1\t1.5135e-06\t5.8155e-05\tFalse\n+ABCF1_p30545878\tABCF1\t2012.3/1989.1\t1064.3/432\t2000.7\t748.15\t-1.4179\t1.0009e+05\t69814\t4.7403\t1.0669e-06\t1\t2.1338e-06\t7.8951e-05\tFalse\n+ABCF1_p30539251\tABCF1\t1127.4/1198.8\t371.59/370.2\t1163.1\t370.9\t-1.6462\t1274\t29895\t4.5817\t2.3062e-06\t1\t4.6124e-06\t0.00016456\tFalse\n+ACTR1A_m104250365\tACTR1A\t402.74/161.61\t711.64/716.37\t282.18\t714.01\t1.3362\t14542\t8970.1\t4.5595\t1\t2.5679e-06\t5.1359e-06\t0.00017692\tTrue\n+ADRBK1_m6703'..b'PH_m64778724\tAFTPH\t49.976/127.87\t105.99/66.945\t88.922\t86.467\t-0.039928\t1897.9\t1774.5\t0.081538\t0.46751\t0.53249\t0.93501\t0.96456\tFalse\n+ADCK3_m227149144\tADCK3\t263.11/168.72\t189.27/231.73\t215.91\t210.5\t-0.036444\t2678.3\t4592\t0.080811\t0.4678\t0.5322\t0.93559\t0.96456\tFalse\n+ABCF1_p30545888\tABCF1\t264.58/353.41\t442.25/191.68\t309\t316.97\t0.036632\t17670\t10451\t0.077983\t0.53049\t0.46951\t0.93902\t0.9661\tTrue\n+ADD3_p111860411\tADD3\t593.83/834.7\t523.01/932.66\t714.26\t727.83\t0.027114\t56457\t30403\t0.077825\t0.53101\t0.46899\t0.93799\t0.96603\tTrue\n+ADARB2_m1779272\tADARB2\t1018.6/513.25\t679.47/824.51\t765.93\t751.99\t-0.026472\t69110\t35557\t0.07398\t0.47051\t0.52949\t0.94103\t0.96717\tFalse\n+ADRA1B_m159343921\tADRA1B\t67.614/245.08\t196.21/110.43\t156.35\t153.32\t-0.028039\t9713\t5375.9\t0.063038\t0.47487\t0.52513\t0.94974\t0.97432\tFalse\n+ACTR3_p114688931\tACTR3\t721.71/768.99\t584.83/888.6\t745.35\t736.72\t-0.016779\t23627\t20022\t0.060988\t0.47568\t0.52432\t0.95137\t0.97432\tFalse\n+AAAS_p53714391\tAAAS\t415.97/708.6\t601.87/537.85\t562.29\t569.86\t0.019263\t22433\t16355\t0.059205\t0.5236\t0.4764\t0.95279\t0.97432\tTrue\n+ADARB1_m46595715\tADARB1\t749.63/378.28\t472.54/673.46\t563.96\t573\t0.022906\t44569\t23763\t0.058653\t0.52333\t0.47667\t0.95335\t0.97432\tTrue\n+ADI1_p3523171\tADI1\t636.45/156.28\t564.02/250.04\t396.37\t407.03\t0.038194\t82286\t33445\t0.058293\t0.51593\t0.48407\t0.96814\t0.97895\tTrue\n+AGPAT3_m45379570\tAGPAT3\t127.88/230.87\t191.16/160.78\t179.38\t175.97\t-0.027492\t2882.6\t3736.7\t0.057892\t0.47692\t0.52308\t0.95383\t0.97432\tFalse\n+AHRR_p344042\tAHRR\t1230.3/525.68\t837.19/945.24\t877.98\t891.22\t0.021562\t1.2703e+05\t56920\t0.055478\t0.52207\t0.47793\t0.95587\t0.9754\tTrue\n+AEN_m89169500\tAEN\t679.08/568.3\t741.3/492.65\t623.69\t616.97\t-0.015604\t18524\t16137\t0.052901\t0.47891\t0.52109\t0.95781\t0.976\tFalse\n+ADCK4_m41220523\tADCK4\t565.9/776.09\t878.2/445.73\t670.99\t661.96\t-0.01952\t57802\t30074\t0.052147\t0.47921\t0.52079\t0.95841\t0.976\tFalse\n+AEBP2_p19615544\tAEBP2\t745.22/751.23\t736.25/773.59\t748.23\t754.92\t0.012832\t357.59\t18298\t0.049483\t0.51973\t0.48027\t0.96053\t0.97716\tTrue\n+AEBP1_p44144359\tAEBP1\t770.21/880.87\t577.9/1056.8\t825.54\t817.36\t-0.014356\t60403\t33744\t0.044558\t0.48223\t0.51777\t0.96446\t0.97895\tFalse\n+AHCTF1_p247079429\tAHCTF1\t601.18/900.41\t735.62/754.13\t750.79\t744.88\t-0.011396\t22470\t19735\t0.042105\t0.48321\t0.51679\t0.96641\t0.97895\tFalse\n+ADNP2_p77875499\tADNP2\t745.22/614.48\t453.61/920.64\t679.85\t687.12\t0.015331\t58803\t30566\t0.041603\t0.51657\t0.48343\t0.96686\t0.97895\tTrue\n+ACTR5_m37377171\tACTR5\t438.02/443.99\t482/391.94\t441\t436.97\t-0.013222\t2036.4\t10162\t0.040007\t0.48404\t0.51596\t0.96809\t0.97895\tFalse\n+ABTB1_m127395860\tABTB1\t945.13/1102.9\t885.77/1174.7\t1024\t1030.2\t0.0087472\t27089\t26326\t0.038419\t0.51532\t0.48468\t0.96935\t0.97895\tTrue\n+ACTL6B_m100253168\tACTL6B\t751.1/939.48\t471.91/1238.2\t845.29\t855.05\t0.016548\t1.5567e+05\t65863\t0.038041\t0.51493\t0.48507\t0.97013\t0.97895\tTrue\n+AEBP2_m19615572\tAEBP2\t1023/468.85\t729.31/774.73\t745.94\t752.02\t0.011697\t77295\t37922\t0.031226\t0.51242\t0.48758\t0.97515\t0.98302\tTrue\n+AFF1_p87967890\tAFF1\t824.6/1007\t526.79/1316.6\t915.78\t921.69\t0.0092723\t1.6426e+05\t70028\t0.022338\t0.50878\t0.49122\t0.98244\t0.98938\tTrue\n+ACHE_p100491656\tACHE\t2626.7/1706.7\t1749.5/2570.2\t2166.7\t2159.8\t-0.0045493\t3.8001e+05\t1.665e+05\t0.016725\t0.49333\t0.50667\t0.98666\t0.9925\tFalse\n+ACHE_m100491816\tACHE\t295.44/420.9\t380.43/338.73\t358.17\t359.58\t0.005641\t4369.4\t8062.6\t0.015671\t0.50624\t0.49376\t0.98753\t0.9925\tTrue\n+ACHE_m100491779\tACHE\t280.75/525.68\t298.41/505.24\t403.21\t401.82\t-0.0049676\t25692\t14696\t0.012018\t0.49521\t0.50479\t0.99041\t0.99439\tFalse\n+AGAP2_m58128444\tAGAP2\t2459.1/1609\t1964.6/2109.6\t2034.1\t2037.1\t0.0021679\t1.8592e+05\t99102\t0.0097214\t0.50388\t0.49612\t0.99224\t0.99523\tTrue\n+ADRB2_p148206450\tADRB2\t608.53/877.32\t955.17/533.27\t742.92\t744.22\t0.0025145\t62561\t32956\t0.0071485\t0.50284\t0.49716\t0.99432\t0.99631\tTrue\n+ACVRL1_m52306881\tACVRL1\t1665.4/1102.9\t1593/1178.1\t1384.1\t1385.6\t0.0015047\t1.2213e+05\t64899\t0.0056737\t0.50226\t0.49774\t0.99547\t0.99647\tTrue\n+ACAD11_m132378564\tACAD11\t989.22/1545.1\t1045.4/1487.1\t1267.1\t1266.2\t-0.0010322\t1.2602e+05\t63931\t0.0035873\t0.49857\t0.50143\t0.99714\t0.99714\tFalse\n' 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diff -r 000000000000 -r b8da4d41aa1d test-data/test1.bam |
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diff -r 000000000000 -r b8da4d41aa1d test-data/test1.fastq.gz |
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