Repository 'getmlst'
hg clone https://toolshed.g2.bx.psu.edu/repos/nml/getmlst

Changeset 0:c2e910327131 (2017-11-17)
Commit message:
planemo upload commit 132092ff7fe1c4810d1221054419389180b81657
added:
getmlst.pl
getmlst.xml
test-data/mlst_database_test.fasta
test-data/mlst_definitions_test.tabular
b
diff -r 000000000000 -r c2e910327131 getmlst.pl
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/getmlst.pl Fri Nov 17 11:49:10 2017 -0500
b
@@ -0,0 +1,34 @@
+#!/usr/bin/env perl
+
+use strict;
+use warnings;
+use Cwd;
+use File::Copy;
+
+my ($mlst_db, $mlst_defs, $species) = @ARGV;
+
+$species =~ s/__pd__/#/ig;
+
+my $command = "getmlst.py --species '$species'";
+
+my $rv = system($command);
+
+if ($rv == 0)
+{
+ #need to find output files in the dir
+ my $cur_dir = getcwd();
+
+ foreach my $file (<$cur_dir/*>)
+ {
+ if ($file =~ /\.fasta$/)
+ {
+ move($file, $mlst_db);
+ }
+ elsif ($file =~/\.txt$/)
+ {
+ move($file, $mlst_defs);
+ }
+ }
+}
+$rv = $rv >> 8;
+exit $rv;
b
diff -r 000000000000 -r c2e910327131 getmlst.xml
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/getmlst.xml Fri Nov 17 11:49:10 2017 -0500
[
@@ -0,0 +1,101 @@
+<tool id="getmlst" name="getmlst" version="0.1.4.1">
+    <description>Download MLST datasets by species from pubmlst.org</description>
+    <requirements>
+        <requirement type="package" version="0.1.4.6">srst2</requirement>
+    </requirements>
+    <stdio>
+        <exit_code range="2"  level="fatal"   description="Please be more specific."/>
+        <exit_code range="1"  level="fatal"   description="No species matched your query."/>
+    </stdio>
+    <command><![CDATA[
+        $__tool_directory__/getmlst.pl $mlst_db $mlst_defs '$species'
+    ]]></command>
+    <inputs>
+        <param name="species" type="text" label="The name of the genus, or genus and species that you want to download." help="Please note you will receive an error if multiple results are returned."/>
+    </inputs>
+    <outputs>
+        <data format="fasta" name="mlst_db" label="MLST Database"/>
+        <data format="tabular" name="mlst_defs" label="MLST Definitions"/>
+    </outputs>
+    <tests>
+        <test>
+            <param name="species" value="Listeria monocytogenes"/>
+            <output name="mlst_db" file="mlst_database_test.fasta" ftype="fasta"/>
+            <output name="mlst_defs" file="mlst_definitions_test.tabular" ftype="tabular"/>
+        </test>
+    </tests>
+    <help>
+
+**GetMLST**
+
+GetMLST_ downloads MLST datasets from pubmlst.org.
+
+Data is downloaded for a species determined by the users
+
+  - profiles (maps STs to allele numbers)
+  - numbered sequences for each locus in the scheme
+
+In addition, the alleles are concatenated together for use with SRST2.
+
+.. _`GetMLST`: https://github.com/katholt/srst2
+
+-----
+
+**What it does**
+
+Searches a database for a given organism and returns the MLST Database(fasta) and MLST Definitions(tabular).
+
+**Usage**
+
+Enter the genus and species of the organism to search and press Execute
+
+**Tips**
+
+1. To produce a list of all species avaialble for a specific genus, enter the genus name and press "Execute".
+
+  - *Helicobacter* will not generate MLST Database or MLST Definition files and an error will occur however the following output will be generated:
+
+  - *The following 3 species match your query, please be more specific:*
+  - Helicobacter cinaedi
+  - Helicobacter pylori
+  - Helicobacter suis
+
+  -
+
+2. To return a list of all species available from a database leave the name blank and press "Execute".
+
+  - Leaving the text field blank will not generate MLST Database or MLST Definition files and an error will occur however the following output will be generated:
+
+  - *The following 114 species match your query, please be more specific:*
+  - Achromobacter spp.
+  - Acinetobacter baumannii#1
+  - Acinetobacter baumannii#2
+  - Aeromonas spp.
+  - Anaplasma phagocytophilum
+  - Arcobacter spp.
+  - Aspergillus fumigatus
+  - Bacillus cereus
+  - Bacillus licheniformis
+  - Bacillus subtilis
+  - Bifidobacterium
+  - Bordetella spp.
+  - Borrelia spp.
+  - Brachyspira hampsonii
+  - Brachyspira hyodysenteriae
+  - Brachyspira intermedia
+  - Brachyspira pilosicoli
+  - Brachyspira spp.
+  - Burkholderia cepacia complex
+  - Burkholderia pseudomallei
+  - Campylobacter concisus/curvus
+  - Campylobacter fetus
+  - Campylobacter helveticus
+  - Campylobacter hyointestinalis
+  - ...
+
+    </help>
+
+    <citations>
+        <citation type="doi">10.1186/s13073-014-0090-6</citation>
+    </citations>
+</tool>
b
diff -r 000000000000 -r c2e910327131 test-data/mlst_database_test.fasta
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/mlst_database_test.fasta Fri Nov 17 11:49:10 2017 -0500
b
b'@@ -0,0 +1,15049 @@\n+>abcZ_1\n+AAATCGACGAACAGAATGCGTATAGGACTTTTCCGCAAGATGGAAAAACTATCAATCCGT\n+TTCTTCGATAGCCGCAATGATGGCGAAATGCTTAGTCGTTTCACTAGTGACTTAGATAAT\n+ATTTCCAACACACTAAACCAAGCATTGATCCAAGTACTATCCAACGTCGCGCTAATGATT\n+GGTGTTATCATCATGATGTTCCAACAAAACGTGGAACTAGCCTTCGTTACTCTAATATCT\n+GCTCCATTTGCGATTATTATTGCGACAGTGATTATTCGAAAAGCCCGCAAATTCGTTGAT\n+ATTCAACAAGATGAACTAGGCGTACTTAACGGCTACATTGACGAAAAAATCTCTGGTCAA\n+AAAATTATTATCACAAATGGCTTAGAAGAAGAAACAATTGACGGCTTTGTTAAACAAAAC\n+AATATCGTTAAAAACGCCACTTACAAAGGTCAAGTTTACTCCGGTTTACTTTTCCCAATG\n+ATGCAAGGTATTTCCCTATTAAATACAGCTATCGTTATCTTCTTCGGTGGATGGTTA\n+>abcZ_2\n+AAATCGACGAACAGAATGCGTATAGGGCTTTTCCGCAAGATGGAAAAACTATCAATCCGT\n+TTCTTCGATAGCCGCAATGATGGCGAAATGCTTAGTCGTTTCACTAGTGACTTAGATAAT\n+ATTTCCAACACACTAAACCAAGCATTGATCCAAGTACTATCCAACGTCGCGCTAATGATT\n+GGTGTTATCATCATGATGTTCCAACAAAACGTGGAACTAGCCTTCGTTACTCTAATATCT\n+GCTCCATTTGCGATTATTATTGCGACAGTGATTATTCGAAAAGCCCGCAAATTCGTTGAT\n+ATTCAACAAGATGAACTAGGCGTACTTAACGGCTACATTGACGAAAAAATCTCTGGTCAA\n+AAAATTATTATCACAAATGGCTTAGAAGAAGAAACAATTGACGGCTTTGTTAAACAAAAC\n+AATATCGTTAAAAACGCCACTTACAAAGGTCAAGTTTACTCCGGTTTACTTTTCCCAATG\n+ATGCAAGGTATTTCCCTATTAAATACAGCTATCGTTATCTTCTTCGGTGGATGGTTA\n+>abcZ_3\n+AAATCGACGAACAGAATGCGTATAGGACTTTTCCGCAAGATGGAAAAACTATCAATCCGT\n+TTCTTCGATAGTCGCAATGATGGCGAAATGCTTAGTCGTTTCACTAGTGACTTAGATAAT\n+ATTTCCAACACACTAAACCAAGCATTGATCCAAGTACTATCCAACGTCGCGCTAATGATT\n+GGTGTTATCATCATGATGTTCCAACAAAACGTGGAACTAGCCTTCGTTACTCTAATATCT\n+GCTCCATTTGCGATTATTATTGCGACAGTGATTATTCGAAAAGCCCGCAAATTCGTTGAT\n+ATTCAACAAGATGAACTAGGCGTACTTAACGGCTACATTGACGAAAAAATCTCTGGTCAA\n+AAAATTATTATCACAAATGGCTTAGAAGAAGAAACAATTGACGGCTTTGTTAAACAAAAC\n+AATATCGTTAAAAACGCAACTTACAAAGGTCAAGTTTACTCCGGTTTACTTTTCCCAATG\n+ATGCAAGGTATTTCCCTATTAAATACAGCTATTGTTATCTTCTTCGGTGGATGGTTA\n+>abcZ_4\n+AAATCGACGAACAGAATGCGTATAGGGCTTTTCCGCAAGATGGAAAAACTATCAATCCGT\n+TTCTTCGATAGCCGCAATGATGGCGAAATGCTTAGCCGCTTCACTAGTGACTTGGATAAT\n+ATTTCCAACACACTAAACCAAGCATTGATCCAAGTACTATCCAACGTCGCGCTAATGATT\n+GGTGTTATCATCATGATGTTCCAACAAAACGTGGAACTAGCCTTCGTTACTCTAATATCT\n+GCTCCATTTGCGATTATTATTGCGACAGTGATTATTCGAAAAGCCCGCAAATTCGTTGAT\n+ATTCAACAAGATGAACTAGGCGTACTTAACGGCTACATTGACGAAAAAATCTCTGGTCAA\n+AAAATTATTATCACAAATGGCTTAGAAGAAGAAACAATTGACGGCTTTGTTAAACAAAAC\n+AATATCGTTAAAAACGCCACTTACAAAGGTCAAGTTTACTCCGGTTTACTTTTCCCAATG\n+ATGCAAGGTATTTCCCTATTAAATACAGCTATCGTTATCTTCTTCGGTGGATGGCTA\n+>abcZ_5\n+AAATCAACGAACAGAATGCGTATAGGGCTTTTCCGCAAGATGGAAAAACTATCGATTCGT\n+TTCTTCGATAGCCGCAATGATGGCGAAATGCTTAGCCGCTTCACTAGCGACTTAGATAAT\n+ATTTCCAATACACTAAACCAAGCATTGATCCAAGTACTATCCAACGTCGCGCTAATGATT\n+GGTGTTATCATCATGATGTTCCAACAAAACGTGGAACTAGCCTTCGTTACTCTAATATCT\n+GCTCCATTTGCAATTATTATTGCGACAGTGATTATTCGAAAAGCACGTAAATTCGTTGAT\n+GTTCAACAAGATGAACTAGGCGTACTTAACGGCTACATTGACGAAAAAATCTCTGGACAA\n+AAAATCATTATCACAAATGGTTTAGAAGAAGAAACAATTGACGGCTTTGTTAAACAAAAC\n+AATATCGTTAAAAACGCCACTTATAAAGGGCAAGTTTACTCCGGTTTACTTTTCCCAATG\n+ATGCAAGGTATTTCCCTATTAAATACAGCTATCGTTATCTTCTTCGGTGGATGGTTA\n+>abcZ_6\n+AAATCGACGAACAGAATGCGTATAGGGCTTTTCCGCAAGATGGAAAAACTATCGATTCGT\n+TTCTTCGATAGCCGCAATGATGGCGAAATGCTTAGTCGTTTTACTAGTGACTTAGATAAT\n+ATTTCCAATACACTAAACCAAGCATTGATCCAGGTGCTATCCAACGTCGCGCTAATGATT\n+GGTGTTATCATCATGATGTTCCAACAAAACGTGGAACTAGCCTTCGTTACTTTAATATCT\n+GCTCCATTTGCGATTATTATTGCAACAGTGATTATTCGAAAAGCACGTAAATTCGTTGAT\n+GTTCAACAAGATGAACTAGGCGTACTTAACGGCTACATTGACGAAAAAATCTCTGGACAA\n+AAAATCATTATCACAAATGGTTTAGAAGAAGAAACAATTGACGGCTTTGTTAAACAAAAC\n+AATATCGTTAAAAACGCCACTTATAAAGGGCAAGTTTACTCCGGTTTACTTTTCCCAATG\n+ATGCAAGGTATTTCACTATTAAATACAGCTATCGTTATCTTCTTCGGTGGATGGTTA\n+>abcZ_7\n+AAATCGACGAACAGAATGCGTATAGGGCTTTTCCGCAAGATGGAAAAACTATCGATTCGT\n+TTCTTCGATAGCCGCAATGATGGCGAAATGCTTAGCCGCTTCACTAGTGACTTAGATAAT\n+ATTTCCAATACACTAAACCAAGCATTGATCCAAGTACTATCCAACGTCGCGCTAATGATT\n+GGTGTTATCATCATGATGTTCCAACAAAACGTGGAACTAGCCTTCGTTACTCTAATATCT\n+GCTCCATTTGCAATTATTATTGCGACAGTGATTATTCGAAAAGCACGTAAATTCGTTGAT\n+GTTCAACAAGATGAACTAGGCGTACTTAACGGCTACATTGACGAAAAAATCTCTGGACAA\n+AAAATCATTATCACAAATGGTTTAGAAGAAGAAACAATTGACGGCTTTGTTAAACAAAAC\n+AATATCGTTAAAAACGCCACTTATAAAGGGCAAGTTTACTCCGGTTTACTTTTCCCAATG\n+ATGCAAGGTATTTCCCTATTAAATACAGCTATCGTTATCTTCTTCGGTGGATGGTTA\n+>abcZ_8\n+AAATCGACGAACAGAATGCGT'..b'TCTTCGGAAGATAAG\n+AAAAAACTGGAAAGCGGCGAAACGGTTAGCAAGAAAATAGATAATCGATTCAATAGAGAA\n+ATGACGATTGTTTATGTGCCGATTATGGATGGTGATAAATTTGTCGGTTCTATCGTGCTG\n+AATTCACCTATTAGCGGGACCGAGCAAGTAATTGGTACTATTAATCGTTACATGTTTTAT\n+ACTATTTTACTTTCCATAACCGTAGCACTTATTCTTAGTGCGATTTTGTCCAAACTACAA\n+GTGAATAGAATCAACAAACTACGAGCAGCGACGAAAGATGTTATTCAAGGCAACTACAAA\n+GCCAGACTAAAAGAAAATAACTTTGATGAAATTGGCGCGCTTGCGATTGATTTCAATAAG\n+ATGACTCAAACACTGGAAATTTCTCAAGAAGAAATTGAAAGACAAGAAAAACGGCGCCGT\n+>lhkA_180\n+TATCCAACACAAATGGACCAGCCTCTACCTAAAGACTTTTCGATTTCTTCGGAAGATAAG\n+AAAAAACTGGAAAGCGGCGAAACGGTTAGCAAGAAAATAGATAATCGATTCAATAGAGAA\n+ATGACGATTGTTTATGTGCCGATTATGGATGGTGATAAATTTGTCGGTTCTATCGTGCTG\n+AATTCACCTATTAGCGGGACCGAGCAAGTAATTGGTACGATTAATCGTTATATGTTCTAT\n+ACTATTTTACTCTCCATAACAGTAGCACTTATTCTTAGTGCGATATTGTCCAAACTACAA\n+GTGAATCGAATCAACAAACTACGAGCAGCGACGAAAGATGTTATTCAAGGCAACTACAAA\n+GCCAGACTAAAAGAAAATAACTTTGATGAAATTGGTGCGCTTGCGATTGATTTCAATAAG\n+ATGACTCAAACACTGGAAATTTCTCAAGAAGAAATTGAAAGACAAGAAAAACGGCGCCGT\n+>lhkA_181\n+TACCCAACCCAGCTAAATCAGCCATTACCCAAGGACTTCTCTATTTCATCTGATGATAAG\n+AAAAAATTAGAAAGTGGCGAAACGGTAAGTAAAAAGATTGATAATCGTTTTAATAGAGAA\n+ATGACGATTGTGTATGTTCCGATTATGGATGGCAGCAAATTTGTTGGTTCAATCGTGCTG\n+AATTCGCCGATAAGCGGCACCCAGCAAGTCATAGGTACAATTAATCGTTATATGTTCTAC\n+ACTATTTTGCTTTCTATCACTGTGGCACTTATCCTTAGCGCGATTTTATCCAAGCTGCAA\n+GTCAATCGGATTAATAAACTGCGTGCTGCCACCAAAGATGTTATTCAAGGTAATTACAAA\n+GCCAGACTAAAAGAAAATAACTTCGATGAGATCGGCGCGCTTGCTATTGATTTCAATAAA\n+ATGACGCAAACACTCGAAACGTCCCAAGAAGAAATTGAACGTCAAGAAAAACGGCGTCGT\n+>lhkA_182\n+TATCCAACCCAGCTAAATCAGCCATTACCCAAGGACTTCTCTATTTCATCTGATGATAAG\n+AAAAAATTAGAAAGTGGCGAAACGGTAAGTAAAAAGATTGATAATCGTTTTAATAGAGAA\n+ATGACGATTGTGTATGTTCCGATTATGGATGGCAGCAAATTTGTTGGTTCAATCGTGCTG\n+AATTCGCCGATAAGCGGCACCCAGCAAGTCATAGGTACAATTAATCGTTATATGTTCTAC\n+ACTATTTTGCTTTCTATCACTGTGGCACTTATCCTTAGCGCGATTTTATCCAAGCTGCAA\n+GTCAATCGGATTAATAAACTGCGTGCTGCCACCAAAGATGTTATTCAAGGTAATTACAAA\n+GCCAGACTAAAAGAAAATAACTTCGATGAGATCGGCGCGCTTGCTATTGATTTCAATAAA\n+ATGACGCAAACACTCGAAACGTCCCAAGAAGAAATTGAACGTCAAGAAAAACGGCGTCGT\n+>lhkA_183\n+ATTTATCCGAGTTCAAGTCGTCCACTCCCACCTGATTTTAAAATTTCAGACGCGGATATG\n+AAAAAATTAAAAAAAGGTGAAACCGTGACAATTAAAGTGGACAGTCGTTTTAACCAGGGA\n+ATGTCTGTCGTTTATGTGCCTATTATGAACGGGGAAAGCTATTTGGGTGCGGTTATTTTA\n+AATGCTCCGATTAGTGGGACGGAAAAAGTGGTTGGGACGATTAACCAATATATGTTCTAT\n+GCGATTCTGATTTCCATTCTGGTAGCTTTGATTTTAAGTTTCCTAATGTCGAAGCTTCAA\n+GTCAATCGAATTAATAAACTGCGGGACGCAACCAAGCGTGTGACGCGCGGGGATTATGAG\n+ACGCGGTTAAAAGAAAATAAGGTGGATGAAATTGGCGAACTGGCGCACGATTTTAACCGT\n+ATGACGGAAAATTTAGCGGAATCCCGCGAAGAAATTGAACGTCAAGAGCGCCGCAGAAGA\n+>lhkA_184\n+TATCCAACACAGATGAATCAGCCGTTACCAAAGGATTTCTCTATTTCTTCGGATGATAAG\n+AAAAAGCTTGAAAGTGGCGAAACAGTTAGTAAGAAAATAGATAATCGCTTTAATAAAGAA\n+ATGACAATTGTGTACCTCCCAATAATGAATGGCGACAAATTTGTCGGTTCTATCGTGCTC\n+AATTCACCTATTAGCGGTACGAAGCAAGTAATTGGTACGATTAACCGCTATATGTTCTAC\n+ACTATTTTACTTTCTATAACGGTAGCACTTATTCTTAGCGCAATCTTGTCCAAACTACAA\n+GTAAATCGAATCAACAAACTACGAGCAGCGACAAAAGACGTTATTCAAGGCAATTACAAA\n+GCTCGATTGAAGGAAAATAATTTTGATGAAATTGGTGCACTTGCCATTGATTTCAATAAA\n+ATGACACAAACCCTTGAAACATCTCAAGAAGAAATAGAACGACAAGAGAAGCGGAGACGC\n+>lhkA_185\n+TATCCAACACAGATGAATCAGCCGTTACCAAAGGATTTCTCTATTTCTTCGGATGATAAG\n+AAAAAACTTGAAAGTGGCGAAACAGTTAGTAAGAAAATAGATAATCGCTTTAATAAAGAA\n+ATGACAATTGTGTACGTCCCAATAATGAATGGCGACAAATTTGTCGGTTCTATCGTGCTC\n+AATTCACCTATTAGCGGTACGGAGCAAGTAATTGGTACGATTAACCGCTATATGTTCTAC\n+ACTATTTTACTTTCTATAACGGTAGCACTTATTCTTAGCGCAATCTTGTCCAAACTACAA\n+GTAAATCGAATCAACAAACTACGAGCAGCGACAAAAGACGTTATTCAAGACAATTACAAA\n+GCTCGATTGAAGGAAAATAATTTTGATGAAATTGGTGCACTTGCCATTGATTTCAATAAA\n+ATGACACAAACCCTTGAAACATCTCAAGAAGAAATAGAACGACAAGAGAAGCGGAGACGC\n+>lhkA_186\n+TATCCAACACAGATGAATCAGCCTTTACCTAAGGATTTCTCTATTTCTGCGGATGATAAG\n+AAAAAGCTTGAAAGTGGTGAAACGGTTAGTAAAAAAATAGATAATCGCTTTAACAAAGAA\n+ATGACAATTGTGTACGTCCCAATAATGAATGGCGATAAATTTGTCGGTTCTATCGTGCTG\n+AATTCACCCATTAGCGGTACGGAGCAAGTAATTGGCACGATTAACCGCTATATGTTCTAC\n+ACTATTTTACTTTCTATAACGGTAGCACTTATTCTTAGCGCAATCTTGTCCAAACTACAA\n+GTAAATCGAATCAACAAACTACGAGCAGCGACAAAAGACGTTATTCAAGGCAATTACAAC\n+GCTCGCTTGAAGGAAAATAATTTTGATGAAATTGGTGCACTCGCCATTGATTTCAATAAA\n+ATGACACAAACCCTTGAAACATCTCAAGAAGAAATTGAACGACAAGAGAAACGGAGACGC\n'
b
diff -r 000000000000 -r c2e910327131 test-data/mlst_definitions_test.tabular
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/mlst_definitions_test.tabular Fri Nov 17 11:49:10 2017 -0500
b
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