Next changeset 1:46507a10106c (2019-06-24) |
Commit message:
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/gsc_cpm_tpm_rpk commit 09dcd74dbc01f448518cf3db3e646afb0675a6fe |
added:
cpm_tpm_rpk.R cpm_tpm_rpk.xml test-data/counts.tab test-data/cpm.tab test-data/gene_length.tab test-data/logcpm.tab test-data/logrpk.tab test-data/logtpm.tab test-data/none.tab test-data/rpk.tab test-data/tpm.tab |
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diff -r 000000000000 -r ce3d027ec26b cpm_tpm_rpk.R --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/cpm_tpm_rpk.R Mon Jun 24 13:37:16 2019 -0400 |
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@@ -0,0 +1,149 @@ +if (length(commandArgs(TRUE)) == 0) { + system("Rscript cpm_tpm_rpk.R -h", intern = F) + q("no") +} + + +# load packages that are provided in the conda env +options( show.error.messages=F, + error = function () { cat( geterrmessage(), file=stderr() ); q( "no", 1, F ) } ) +loc <- Sys.setlocale("LC_MESSAGES", "en_US.UTF-8") +warnings() +library(optparse) + +#Arguments +option_list = list( + make_option( + c("-d", "--data"), + default = NA, + type = 'character', + help = "Input file that contains count values to transform" + ), + make_option( + c("-t", "--type"), + default = 'cpm', + type = 'character', + help = "Transformation type, either cpm, tpm, rpk or none[default : '%default' ]" + ), + make_option( + c("-s", "--sep"), + default = '\t', + type = 'character', + help = "File separator [default : '%default' ]" + ), + make_option( + c("-c", "--colnames"), + default = TRUE, + type = 'logical', + help = "Consider first line as header ? [default : '%default' ]" + ), + make_option( + c("-f", "--gene"), + default = NA, + type = 'character', + help = "Two column of gene length file" + ), + make_option( + c("-a", "--gene_sep"), + default = '\t', + type = 'character', + help = "Gene length file separator [default : '%default' ]" + ), + make_option( + c("-b", "--gene_header"), + default = TRUE, + type = 'logical', + help = "Consider first line of gene length as header ? [default : '%default' ]" + ), + make_option( + c("-l", "--log"), + default = FALSE, + type = 'logical', + help = "Should be log transformed as well ? (log2(data +1)) [default : '%default' ]" + ), + make_option( + c("-o", "--out"), + default = "res.tab", + type = 'character', + help = "Output name [default : '%default' ]" + ) +) + +opt = parse_args(OptionParser(option_list = option_list), + args = commandArgs(trailingOnly = TRUE)) + +if (opt$sep == "tab") {opt$sep = "\t"} +if (opt$gene_sep == "tab") {opt$gene_sep = "\t"} + +cpm <- function(count) { + t(t(count) / colSums(count)) * 1000000 +} + + +rpk <- function(count, length) { + count / (length / 1000) +} + +tpm <- function(count, length) { + RPK = rpk(count, length) + perMillion_factor = colSums(RPK) / 1000000 + TPM = RPK / perMillion_factor + return(TPM) +} + +data = read.table( + opt$data, + check.names = FALSE, + header = opt$colnames, + row.names = 1, + sep = opt$sep +) + +if (opt$type == "tpm" | opt$type == "rpk") { + gene_length = as.data.frame( + read.table( + opt$gene, + h = opt$gene_header, + row.names = 1, + sep = opt$gene_sep + ) + ) + gene_length = as.data.frame(gene_length[match(rownames(data), rownames(gene_length)), ], rownames(data)) +} + + +if (opt$type == "cpm") + res = cpm(data) +if (opt$type == "tpm") + res = as.data.frame(apply(data, 2, tpm, length = gene_length), row.names = rownames(data)) +if (opt$type == "rpk") + res = as.data.frame(apply(data, 2, rpk, length = gene_length), row.names = rownames(data)) +if (opt$type == "none") + res = data +colnames(res) = colnames(data) + + +if (opt$log == TRUE) { + res = log2(res + 1) +} + +write.table( + cbind(Genes = rownames(res), res), + opt$out, + col.names = opt$colnames, + row.names = F, + quote = F, + sep = "\t" +) + + + + + + + + + + + + |
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diff -r 000000000000 -r ce3d027ec26b cpm_tpm_rpk.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/cpm_tpm_rpk.xml Mon Jun 24 13:37:16 2019 -0400 |
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@@ -0,0 +1,160 @@ +<tool id="cpm_tpm_rpk" name="Generate CPM, TPM, RPK" version="0.9.0"> + <description>from raw counts expression values</description> + <requirements> + <requirement type="package" version="1.6.0">r-optparse</requirement> + </requirements> + <stdio> + <exit_code range="1:" level="fatal" description="Tool exception" /> + </stdio> + <command detect_errors="exit_code"><![CDATA[ + Rscript $__tool_directory__/cpm_tpm_rpk.R + --data '$input' + --type '$option.type_transfo' + --sep '$input_sep' + --colnames '$input_header' + #if $option.type_transfo == "tpm": + -f '$option.gene_file' + --gene_sep $option.gene_sep + --gene_header $option.gene_header + #end if + #if $option.type_transfo == "rpk": + -f '$option.gene_file' + --gene_sep '$option.gene_sep' + --gene_header '$option.gene_header' + #end if + --log '$log' + #if $log == "FALSE": + -o ${output} + #else if $log == "TRUE": + -o ${output_log} + #end if +]]></command> + <inputs> + <param name="input" type="data" format="txt,tabular" label="Raw counts of expression data"/> + <param name="input_sep" type="select" label="Input column separator"> + <option value="tab" selected="true">Tabulation</option> + <option value=",">Comma</option> + </param> + <param name="input_header" type="select" label="Consider first line of input file as header?"> + <option value="TRUE" selected="true">Yes</option> + <option value="FALSE">No</option> + </param> + <conditional name="option"> + <param name="type_transfo" type="select" label="Type of transformation"> + <option value="cpm" selected="true">CPM</option> + <option value="tpm">TPM</option> + <option value="rpk">RPK</option> + </param> + <when value="tpm"> + <param name="gene_file" type="data" format="txt,tabular" label="Gene length file"/> + <param name="gene_sep" type="select" label="Gene length column separator"> + <option value="tab" selected="true">Tabulation</option> + <option value=",">Commas</option> + </param> + <param name="gene_header" type="select" label="Consider first line of gene length file as header ?"> + <option value="TRUE" selected="true">Yes</option> + <option value="FALSE">No</option> + </param> + </when> + <when value="rpk"> + <param name="gene_file" type="data" format="txt,tabular" label="Gene length file"/> + <param name="gene_sep" type="select" label="Gene length column separator"> + <option value="tab" selected="true">Tabs</option> + <option value=",">Commas</option> + </param> + <param name="gene_header" type="select" label="Consider first line of gene length file as header ?"> + <option value="TRUE" selected="true">Yes</option> + <option value="FALSE">No</option> + </param> + </when> + <when value="cpm"> + </when> + </conditional> + <param name="log" type="select" label="Data should be log transformed ?"> + <option value="FALSE" selected="true">No</option> + <option value="TRUE">Yes</option> + </param> + </inputs> + <outputs> + <data name="output" format="tabular" label="${option.type_transfo} from ${on_string}"> + <filter>log == "FALSE"</filter> + </data> + <data name="output_log" format="tabular" label="log2(${option.type_transfo} +1) from ${on_string}"> + <filter>log == "TRUE"</filter> + </data> + </outputs> + <tests> + <test> + <param name="input" value="counts.tab" ftype="tabular"/> + <param name="type_transfo" value="cpm"/> + <param name="log" value="TRUE"/> + <output name="output" file="logcpm.tab" ftype="tabular"/> + </test> + <test> + <param name="input" value="counts.tab" ftype="tabular"/> + <param name="type_transfo" value="tpm"/> + <param name="gene_file" value="gene_length.tab" ftype="tabular"/> + <param name="log" value="TRUE"/> + <output name="output" file="logtpm.tab" ftype="tabular"/> + </test> + <!-- test without t-SNE --> + <test> + <param name="input" value="counts.tab" ftype="tabular"/> + <param name="type_transfo" value="cpm"/> + <output name="output" file="cpm.tab" ftype="tabular"/> + </test> + <test> + <param name="input" value="counts.tab" ftype="tabular"/> + <param name="type_transfo" value="tpm"/> + <param name="gene_file" value="gene_length.tab" ftype="tabular"/> + <param name="gene_header" value="TRUE"/> + <output name="output" file="tpm.tab" ftype="tabular"/> + </test> + <test> + <param name="input" value="counts.tab" ftype="tabular"/> + <param name="type_transfo" value="rpk"/> + <param name="gene_file" value="gene_length.tab" ftype="tabular"/> + <param name="gene_header" value="TRUE"/> + <output name="output" file="rpk.tab" ftype="tabular"/> + </test> + <test> + <param name="input" value="counts.tab" ftype="tabular"/> + <param name="type_transfo" value="rpk"/> + <param name="gene_file" value="gene_length.tab" ftype="tabular"/> + <param name="gene_header" value="TRUE"/> + <param name="log" value="TRUE"/> + <output name="output" file="logrpk.tab" ftype="tabular"/> + </test> + </tests> + <help> + +**What it does** + +Takes a raw count expression matrix and returns a table of normalized expression values. + +Normalization can be: + +- CPM (Counts Per Million) are obtained by dividing counts by the library counts sum and multiplying the results by a million. +- RPK (Reads Per Kilobases) are obtained by dividing read counts by gene lengths (expressed in kilo-nucleotides). +- TPM (Transcripts Per Million) are obtained by dividing RPK values by the sum of all RPK values in a sample and multiplying the results by 1 million. + +RPK and TPM require a two-column correspondance table gene_name - gene length where the length is specified in nucleotide. Both these metrics are relevant only for sequencing of full length RNAs. + +Note: First header row must NOT start with a '#' comment character + +Computed values may be base-2 log-transformed (log2([CPM or RPK or TPM]+1)) + + </help> + <citations> + <citation type="bibtex"> + @Manual{, + title = {R: A Language and Environment for Statistical Computing}, + author = {{R Core Team}}, + organization = {R Foundation for Statistical Computing}, + address = {Vienna, Austria}, + year = {2014}, + url = {http://www.R-project.org/}, + } + </citation> + </citations> +</tool> |
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diff -r 000000000000 -r ce3d027ec26b test-data/counts.tab --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/counts.tab Mon Jun 24 13:37:16 2019 -0400 |
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No newline at end of file\n' |
b |
diff -r 000000000000 -r ce3d027ec26b test-data/cpm.tab --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/cpm.tab Mon Jun 24 13:37:16 2019 -0400 |
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b'@@ -0,0 +1,35194 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|
b |
diff -r 000000000000 -r ce3d027ec26b test-data/gene_length.tab --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/gene_length.tab Mon Jun 24 13:37:16 2019 -0400 |
b |
b'@@ -0,0 +1,53673 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No newline at end of file\n' |
b |
diff -r 000000000000 -r ce3d027ec26b test-data/logcpm.tab --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/logcpm.tab Mon Jun 24 13:37:16 2019 -0400 |
b |
b'@@ -0,0 +1,35194 @@\n+Genes\tWt1\tWt2\tWt3\tCA1\tCA2\tCA3\tKK1\tKK2\tKK3\n+0610005C13Rik\t2.23845505996379\t1.91731740892465\t1.93418850835685\t1.84234493437827\t1.83542397244854\t1.87237456532778\t2.33872429523358\t2.02545074537792\t2.04190046760359\n+0610006L08Rik\t0\t0\t0\t0\t0\t0.0669833753741182\t0\t0\t0\n+0610009B22Rik\t4.66628534001215\t4.67155988576351\t4.87385332364942\t4.50734252383895\t4.44671793939391\t4.47837500916089\t4.72202672270845\t4.69578628096291\t4.63234106633311\n+0610009E02Rik\t3.49591321893519\t3.2989075262378\t3.37926563323017\t3.3849531286591\t3.48847330148664\t3.57082827558175\t3.3371008104431\t3.18963392729766\t3.19660034761207\n+0610009L18Rik\t1.33988862970429\t1.33930514655114\t0.834942874768512\t1.03947477733719\t0.982677729481796\t0.96383003485809\t0.699900287394128\t0.842025675982355\t0.568568450921874\n+0610009O20Rik\t5.35293334422573\t5.43945085134167\t5.49675955325264\t5.52241406104713\t5.51886860255227\t5.42504773158453\t5.41190531870063\t5.37745128195265\t5.4947165577597\n+0610010F05Rik\t5.30583900661438\t5.11074397995404\t5.31744176593288\t4.97259162235934\t5.22988802440403\t5.07656228635046\t5.2944017269107\t5.17672509347099\t5.10326471575511\n+0610010K14Rik\t0.948397994612813\t0.539452168200853\t0.834942874768512\t1.0760481213778\t1.15898718804954\t1.24986860692394\t0.953517638957561\t1.02874025579343\t0.909408300282999\n+0610012D04Rik\t0.14975341489191\t0\t0.0734808373491687\t0\t0.0722776838678355\t0.130994257642744\t0.209263858889789\t0.199883995613306\t0\n+0610012G03Rik\t4.8138427729139\t4.940121789925\t5.02041157366506\t4.45275937478025\t4.6814561172749\t4.45980095301208\t4.64010298989544\t4.87207393901999\t4.66017013737629\n+0610030E20Rik\t4.51576089894109\t4.33321020917173\t4.30822060602863\t4.29880360049849\t4.2465098524733\t4.34977677214734\t4.39455483821255\t4.25401920425035\t4.34808817232356\n+0610033M10Rik\t0\t0\t0\t0.144761560980538\t0\t0.0669833753741182\t0.142825659307661\t0.0697507340449269\t0.0620025184110997\n+0610037L13Rik\t4.5397046128383\t4.6423902070947\t4.40391098648157\t4.58235564578128\t4.44331507125803\t4.48144756851238\t4.46082215069385\t4.70129078645212\t4.37886539513063\n+0610038B21Rik\t1.62209492792856\t1.30662193574653\t1.60304809787323\t2.14740681132666\t1.91619522855864\t2.20533879015479\t1.78472900177206\t1.7347250406797\t1.6359215094862\n+0610039K10Rik\t0.948397994612813\t0.887652731058775\t0.876596572982451\t1.0760481213778\t1.37441792813978\t1.60574767766981\t1.46942347654025\t1.42449285123566\t1.26618085075248\n+0610040B10Rik\t0.62937919432059\t0.973441249292143\t0.834942874768512\t1.14652560001178\t0.64628045818068\t0.96383003485809\t0.914224717183301\t0.956927432020159\t0.525204435623763\n+0610040F04Rik\t1.30837709993252\t1.33930514655114\t1.17252479855995\t1.14652560001178\t1.43049048969242\t1.65011314265591\t1.32708290666077\t1.39762004526308\t1.23959737813155\n+0610040J01Rik\t1.13958622224797\t0.887652731058775\t1.10403694797339\t1.75481406782762\t1.81450425686275\t1.83442767571444\t1.84865217894046\t1.87770809095193\t1.71524103918457\n+0610043K17Rik\t0.467623226891532\t0.226548838989738\t0.655147270133281\t0.276313038638934\t0.141106424760476\t0.251078226268554\t0.391983513242642\t0.481591017194141\t0.233464757024114\n+1010001B22Rik\t0.409406711585629\t0.422303390608671\t0.449711369704472\t0.276313038638934\t0.496720064731575\t0.513709538332349\t0.27277670556514\t0.375414261523614\t0.286371086853762\n+1010001N08Rik\t0.14975341489191\t0.226548838989738\t0.273825829470238\t0.144761560980538\t0\t0\t0.27277670556514\t0.199883995613306\t0.233464757024114\n+1110002E22Rik\t0\t0\t0\t0.144761560980538\t0\t0\t0.073179561658965\t0\t0.0620025184110997\n+1110002J07Rik\t2.48386821453942\t2.3768848164447\t2.5378485077701\t3.59485087069609\t3.49506202582972\t3.74967399249095\t3.14680821302994\t3.01498943637089\t3.06652149505353\n+1110002L01Rik\t4.52264260519467\t4.44254467578508\t4.44602233419789\t4.23149574201992\t4.25040940347267\t4.35313545599306\t4.36935231432273\t4.38975529506319\t4.1374541261063\n+1110002O04Rik\t0\t0\t0\t0.0741957104952366\t0\t0.0669833753741182\t0\t0\t0\n+1110003F10Rik\t0.906920393532013\t0.35995719365727\t0.956460912948477\t0.660393416042979\t0.443210680541945\t0.414317839771467\t0.33361098477862\t'..b'0\t0\t0\n+n-R5s192\t0.285413679674889\t0.079535217295741\t0.0734808373491687\t0\t0\t0.0669833753741182\t0.073179561658965\t0\t0.121449801559577\n+n-R5s194\t0\t0.079535217295741\t0\t0\t0.0722776838678355\t0\t0\t0\t0\n+n-R5s196\t0.14975341489191\t0.35995719365727\t0.0734808373491687\t0\t0.329842633696389\t0.0669833753741182\t0.073179561658965\t0.136284144651557\t0.121449801559577\n+n-R5s197\t0.14975341489191\t0\t0\t0.212036213183989\t0.0722776838678355\t0.0669833753741182\t0\t0.0697507340449269\t0.0620025184110997\n+n-R5s200\t0.467623226891532\t0.226548838989738\t0.449711369704472\t0.212036213183989\t0.269632866353377\t0.192285293062172\t0.448085803139563\t0.0697507340449269\t0.178544151384231\n+n-R5s201\t0.219177519899899\t0.226548838989738\t0.210086180037385\t0.212036213183989\t0.141106424760476\t0.464869444812885\t0.073179561658965\t0.136284144651557\t0.0620025184110997\n+n-R5s202\t0.285413679674889\t0.154913842660267\t0.143399759186283\t0.144761560980538\t0.206800438784241\t0.307568742991556\t0.33361098477862\t0.199883995613306\t0.233464757024114\n+n-R5s205\t0\t0\t0.0734808373491687\t0.0741957104952366\t0\t0.130994257642744\t0\t0\t0.121449801559577\n+n-R5s207\t0\t0\t0\t0\t0\t0.0669833753741182\t0.33361098477862\t0\t0.0620025184110997\n+n-R5s21\t0.0768189079616122\t0\t0\t0\t0\t0\t0.073179561658965\t0\t0.121449801559577\n+n-R5s210\t0\t0\t0\t0\t0\t0\t0.073179561658965\t0\t0\n+n-R5s217\t0\t0\t0\t0.0741957104952366\t0\t0\t0\t0\t0\n+n-R5s24\t0.0768189079616122\t0.079535217295741\t0\t0\t0\t0\t0\t0\t0\n+n-R5s25\t0.0768189079616122\t0\t0\t0\t0\t0\t0\t0.0697507340449269\t0\n+n-R5s26\t0.0768189079616122\t0\t0.210086180037385\t0.144761560980538\t0.141106424760476\t0\t0.073179561658965\t0.0697507340449269\t0.0620025184110997\n+n-R5s29\t0.14975341489191\t0\t0\t0.276313038638934\t0\t0.251078226268554\t0.142825659307661\t0\t0.121449801559577\n+n-R5s31\t0.14975341489191\t0.079535217295741\t0.0734808373491687\t0.0741957104952366\t0\t0.0669833753741182\t0.142825659307661\t0.0697507340449269\t0\n+n-R5s32\t0.0768189079616122\t0\t0\t0\t0\t0\t0\t0\t0\n+n-R5s39\t0.0768189079616122\t0\t0.143399759186283\t0.276313038638934\t0\t0\t0\t0.136284144651557\t0.0620025184110997\n+n-R5s40\t0.219177519899899\t0.226548838989738\t0.273825829470238\t0.611398662966371\t0.206800438784241\t0.192285293062172\t0.142825659307661\t0.199883995613306\t0.121449801559577\n+n-R5s41\t0\t0.079535217295741\t0\t0.0741957104952366\t0\t0\t0.073179561658965\t0\t0\n+n-R5s46\t0\t0.079535217295741\t0\t0\t0\t0\t0\t0.136284144651557\t0\n+n-R5s47\t0\t0\t0\t0\t0\t0\t0.073179561658965\t0\t0\n+n-R5s48\t0\t0.154913842660267\t0\t0.276313038638934\t0.0722776838678355\t0.0669833753741182\t0\t0\t0\n+n-R5s52\t0\t0.079535217295741\t0.0734808373491687\t0.0741957104952366\t0\t0.130994257642744\t0.27277670556514\t0.0697507340449269\t0\n+n-R5s54\t0\t0\t0\t0\t0.0722776838678355\t0.0669833753741182\t0\t0\t0\n+n-R5s67\t0\t0\t0\t0\t0\t0\t0.073179561658965\t0.0697507340449269\t0\n+n-R5s69\t0\t0\t0\t0\t0\t0\t0\t0\t0.0620025184110997\n+n-R5s73\t0\t0\t0\t0\t0.141106424760476\t0.130994257642744\t0.073179561658965\t0.0697507340449269\t0.0620025184110997\n+n-R5s74\t0\t0\t0\t0\t0.0722776838678355\t0.0669833753741182\t0\t0\t0\n+n-R5s76\t0.219177519899899\t0\t0\t0.276313038638934\t0\t0.130994257642744\t0.27277670556514\t0.429479193638114\t0.0620025184110997\n+n-R5s80\t0.0768189079616122\t0\t0\t0.0741957104952366\t0.0722776838678355\t0\t0\t0\t0.0620025184110997\n+n-R5s84\t0.0768189079616122\t0.079535217295741\t0.0734808373491687\t0\t0\t0\t0\t0.136284144651557\t0.0620025184110997\n+n-R5s85\t0.285413679674889\t0\t0\t0\t0.206800438784241\t0.130994257642744\t0\t0.199883995613306\t0.0620025184110997\n+n-R5s87\t0.0768189079616122\t0\t0\t0.144761560980538\t0\t0.130994257642744\t0\t0\t0.0620025184110997\n+n-R5s89\t0\t0\t0.0734808373491687\t0\t0\t0\t0\t0\t0\n+n-R5s96\t0\t0\t0\t0\t0\t0\t0\t0.0697507340449269\t0\n+n-R5s97\t0.219177519899899\t0.294794528129398\t0\t0.0741957104952366\t0.141106424760476\t0.130994257642744\t0.073179561658965\t0\t0.0620025184110997\n+n-TSaga9\t0.285413679674889\t0.226548838989738\t0.0734808373491687\t0.212036213183989\t0.0722776838678355\t0.130994257642744\t0.142825659307661\t0.136284144651557\t0.0620025184110997\n+n-TStga1\t0.409406711585629\t0.422303390608671\t0.334868150513639\t0.396865055178037\t0.329842633696389\t0.414317839771467\t0.33361098477862\t0.429479193638114\t0.386696461951873\n' |
b |
diff -r 000000000000 -r ce3d027ec26b test-data/logrpk.tab --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/logrpk.tab Mon Jun 24 13:37:16 2019 -0400 |
b |
b'@@ -0,0 +1,35194 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|
b |
diff -r 000000000000 -r ce3d027ec26b test-data/logtpm.tab --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/logtpm.tab Mon Jun 24 13:37:16 2019 -0400 |
b |
b'@@ -0,0 +1,35194 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|
b |
diff -r 000000000000 -r ce3d027ec26b test-data/none.tab --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/none.tab Mon Jun 24 13:37:16 2019 -0400 |
b |
b'@@ -0,0 +1,216 @@\n+Features\t20nM_1\t20sM_4\t32nM_7\t32sM_10\t40nM_13\t40sM_16\t20n1d_a\t20n1d_b\t30n5h_c1\t30n5h_c2\t30s5h_c3\t30s5h_c4\t40n5h_c5\t40n5h_c6\t40s5h_c7\t40s5h_c8\t20n1d_c\t20s1d_d\t20s1d_e\t20s1d_f\t30n1d_g\t30n1d_h\t30n1d_i\t30s1d_j\t30s1d_k\t30s1d_l\t40n1d_m\t40n1d_n\t40n1d_o\t40s1d_p\t40s1d_q\t40s1d_r\n+5S_DM\t0\t0\t0\t0\t2.52271103749722\t0\t0\t1.46876851779219\t1.10607886502204\t0\t0\t0\t0\t0\t0\t0\t0\t0\t0\t0\t0\t0\t0\t0\t0\t1.51278181441303\t2.52260597944523\t0\t0\t0\t2.05794942496048\t0.646139123391797\n+ACCORD2_I-int\t13.4694287027486\t19.7873189245625\t17.5674602110498\t50.6988197972739\t17.6589772624805\t19.313391508794\t18.1589910793351\t13.2189166601297\t12.1668675152425\t18.2343843151734\t26.0100186444685\t27.0989940048899\t23.1050300244153\t31.4819885379018\t24.4065408703064\t27.0644930469596\t9.83737686997288\t16.0386739801143\t14.5565923551731\t13.0633061628418\t25.016895793462\t24.992288899494\t17.9393955029792\t28.3792668127381\t19.8019745551662\t35.5503726387062\t17.6582418561166\t22.6167312355617\t26.939353284346\t26.4137500118019\t25.7243678120059\t41.9990430204668\n+ACCORD2_LTR\t0\t0.860318214111411\t0\t2.25328087987884\t3.78406655624583\t2.75905592982771\t1.13493694245845\t0\t2.21215773004408\t0\t4.0640654131982\t3.69531736430316\t1.84840240195322\t4.49742693398597\t1.10938822137756\t2.00477726273775\t0\t1.33655616500952\t0.970439490344876\t2.61266123256835\t4.46730282026107\t3.22481147090245\t2.4462812049517\t2.74638065929724\t2.47524681939578\t1.51278181441303\t0\t10.3660018162991\t2.78682965010476\t0\t1.02897471248024\t5.16911298713437\n+ACCORD_I-int\t16.1633144432983\t28.3905010656766\t29.6450891061465\t33.7992131981826\t18.9203327812291\t43.2252095673008\t30.643297446378\t19.0939907312984\t11.0607886502204\t53.8743172948305\t18.6947009007117\t23.4036766405867\t36.0438468380879\t25.1855908303214\t25.5159290916839\t48.114654305706\t16.3956281166215\t17.3752301451238\t16.4974713358629\t24.8202817093994\t36.6318831261408\t29.8295061058477\t34.2479368693238\t26.5483463732066\t24.7524681939578\t21.9353363089889\t31.5325747430654\t21.6743674340799\t22.2946372008381\t40.0760345006649\t40.1300137867293\t58.7986602286535\n+ACCORD_LTR\t0.897961913516572\t2.58095464233423\t0\t0\t0\t0\t0\t0\t0\t3.3153426027588\t0.812813082639639\t2.46354490953544\t1.84840240195322\t0\t1.10938822137756\t6.01433178821325\t0\t0\t0\t1.30633061628418\t0\t1.61240573545123\t1.63085413663447\t0.915460219765745\t0.825082273131927\t0\t0\t1.88472760296347\t0\t0\t5.14487356240119\t1.93841737017539\n+ALA_DM\t1.79592382703314\t0\t0\t0\t0\t0\t1.13493694245845\t0\t0\t0\t0\t0\t0\t0\t1.10938822137756\t0\t0\t0\t0\t0\t0\t0\t0\t1.83092043953149\t0\t0\t0\t0\t0\t0\t0\t0.646139123391797\n+ARS406_DM\t4.48980956758286\t8.60318214111411\t10.9796626319061\t14.6463257192125\t16.3976217437319\t8.27716778948314\t13.6192433095013\t2.93753703558437\t12.1668675152425\t14.0902060617249\t16.2562616527928\t8.62240718337405\t16.635621617579\t7.19588309437755\t15.5314350992859\t17.0406067332709\t21.8608374888286\t12.0290054850857\t14.5565923551731\t15.6759673954101\t12.508447896731\t18.5426659576891\t14.6776872297102\t11.9009828569547\t8.25082273131927\t18.1533817729563\t18.9195448458392\t14.1354570222261\t13.0052050338222\t6.37573276146942\t7.20282298736166\t14.8611998380113\n+BARI1\t16.1633144432983\t8.60318214111411\t17.5674602110498\t9.01312351951536\t16.3976217437319\t17.4740208889088\t10.214432482126\t10.2813796245453\t4.42431546008817\t11.6036991096558\t7.31531774375675\t17.2448143667481\t6.46940840683628\t8.99485386797193\t4.43755288551025\t14.0334408391642\t14.2095443677386\t13.3655616500952\t17.4679108262078\t23.5139510931152\t5.36076338431329\t11.2868401481586\t8.96969775148958\t15.5628237360177\t11.551151823847\t10.5894727008912\t6.30651494861307\t1.88472760296347\t10.2183753837175\t9.10818965924203\t9.26077241232214\t10.3382259742687\n+BARI_DM\t27.8368193190137\t18.0666824963396\t14.2735614214779\t15.7729661591519\t22.7043993374749\t14.7149649590811\t19.2939280217936\t36.7192129448047\t23.2276561654629\t18.2343843151734\t17.0690747354324\t13.5494970024449\t17.5598228185556\t17.0902223491467\t12.2032704351532\t25.0597157842219\t15.30258624218\t13.3655616500952\t17.4679108262078\t9.14431431398924\t18.7626718450965\t17.7364630899635\t17.9393955029792\t18.'..b'2454821\t46.5943052978576\t54.1289860939192\t45.9077587265401\t68.1643644154857\t70.8420827951759\t16.9822980116943\t50.9272521509762\t67.7210408889515\t48.9256240990341\t52.1812325266475\t48.6798541147837\t37.0631544531192\t34.0551807225106\t55.5994642874225\t50.1629337018857\t50.0950431258312\t23.6664183870455\t59.4447993520453\n+TRANSPAC_LTR\t8.08165722164915\t15.4857278540054\t9.8816963687155\t32.6725727582432\t10.0908441499889\t6.437797169598\t17.0240541368767\t5.87507407116875\t9.95470978519838\t21.5497269179322\t14.6306354875135\t17.2448143667481\t23.1050300244153\t9.89433925476913\t16.6408233206634\t32.076436203804\t10.9304187444143\t22.7214548051619\t22.3201082779321\t1.30633061628418\t12.508447896731\t23.3798831640428\t24.462812049517\t9.15460219765745\t18.9768922820343\t15.8842090513368\t12.6130298972261\t17.904912228153\t25.0814668509429\t20.0380172503325\t10.2897471248024\t27.1378431824555\n+Transib-N1_DM\t14.3673906162651\t12.9047732116712\t7.68576384233428\t4.50656175975768\t10.0908441499889\t12.875594339196\t10.214432482126\t11.7501481423375\t17.6972618403527\t3.3153426027588\t12.1921962395946\t4.92708981907089\t11.0904144117193\t3.59794154718877\t4.43755288551025\t11.0262749450576\t3.27912562332429\t13.3655616500952\t7.76351592275901\t6.53165308142088\t10.7215267686266\t4.83721720635368\t8.15427068317234\t15.5628237360177\t4.95049363879156\t13.6150363297173\t20.1808478355618\t4.71181900740868\t10.2183753837175\t4.55409482962101\t9.26077241232214\t9.04594772748515\n+Transib5\t1.79592382703314\t6.88254571289129\t0\t5.6332021996971\t0\t4.59842654971285\t2.26987388491689\t5.87507407116875\t4.42431546008817\t4.1441782534485\t0\t1.23177245476772\t3.69680480390645\t0.899485386797193\t0\t7.01672041958212\t0\t8.01933699005714\t8.73395541310388\t1.30633061628418\t5.36076338431329\t4.03101433862806\t4.07713534158617\t3.66184087906298\t4.12541136565963\t0.756390907206514\t0\t3.76945520592695\t2.78682965010476\t4.55409482962101\t1.02897471248024\t1.93841737017539\n+XDMR\t9.87758104868229\t12.9047732116712\t6.58779757914366\t1.12664043993942\t6.30677759374304\t6.437797169598\t11.3493694245845\t11.7501481423375\t14.3790252452865\t4.1441782534485\t10.5665700743153\t0\t2.77260360292984\t8.99485386797193\t7.76571754964293\t3.00716589410662\t9.83737686997288\t10.6924493200762\t5.82263694206925\t15.6759673954101\t8.93460564052215\t4.03101433862806\t8.15427068317234\t17.3937441755492\t11.551151823847\t12.8586454225107\t5.04521195889046\t8.48127421333563\t5.57365930020952\t2.73245689777261\t2.05794942496048\t5.16911298713437\n+XDMR_DM\t7.18369530813257\t5.16190928466847\t8.78373010552489\t0\t13.8749107062347\t3.67874123977028\t9.07949553966757\t8.81261110675312\t1.10607886502204\t3.3153426027588\t4.87687849583784\t9.85417963814177\t2.77260360292984\t8.99485386797193\t22.1877644275512\t5.01194315684437\t12.0234606188557\t10.6924493200762\t4.85219745172438\t9.14431431398924\t6.2542239483655\t4.83721720635368\t7.33884361485511\t5.49276131859447\t4.95049363879156\t7.56390907206514\t0\t9.42363801481737\t4.64471608350794\t8.19737069331783\t6.17384827488143\t3.87683474035078\n+ZAM_I-int\t35.0205146271463\t32.6920921362336\t35.1349204220995\t40.5590558378191\t22.7043993374749\t33.1086711579326\t35.1830452162118\t11.7501481423375\t32.0762870856392\t34.8110973289674\t33.3253363882252\t51.7344431002443\t44.3616576468774\t26.0850762171186\t32.1722584199493\t39.0931566233861\t31.6982143588015\t41.4332411152952\t43.6697770655194\t54.8658858839354\t28.5907380496709\t29.0233032381221\t37.5096451425928\t25.6328861534409\t39.6039491103325\t33.2811999170866\t23.9647568047297\t34.8674606548243\t37.1577286680635\t48.2734051939827\t33.9561655118478\t35.5376517865488\n+ZAM_LTR\t1.79592382703314\t1.72063642822282\t2.19593252638122\t3.37992131981826\t3.78406655624583\t1.83937061988514\t2.26987388491689\t0\t7.74255205515429\t17.4055486644837\t4.0640654131982\t1.23177245476772\t12.9388168136726\t4.49742693398597\t7.76571754964293\t3.00716589410662\t5.46520937220715\t2.67311233001905\t2.91131847103463\t6.53165308142088\t5.36076338431329\t0\t0.815427068317234\t0\t3.30032909252771\t4.53834544323909\t10.0904239177809\t7.53891041185389\t7.4315457336127\t7.28655172739362\t3.08692413744071\t3.87683474035078\n' |
b |
diff -r 000000000000 -r ce3d027ec26b test-data/rpk.tab --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/rpk.tab Mon Jun 24 13:37:16 2019 -0400 |
b |
b'@@ -0,0 +1,35194 @@\n+Genes\tWt1\tWt2\tWt3\tCA1\tCA2\tCA3\tKK1\tKK2\tKK3\n+0610005C13Rik\t18.9785096288027\t13.6756907619313\t15.071169411108\t13.6756907619313\t13.9547864917667\t15.6293608707787\t21.769466927156\t17.3039352497907\t19.8157968183087\n+0610006L08Rik\t0\t0\t0\t0\t0\t0.469924812030075\t0\t0\t0\n+0610009B22Rik\t446.89378757515\t432.865731462926\t543.086172344689\t412.825651302605\t405.811623246493\t448.897795591182\t488.977955911824\t504.008016032064\t543.086172344689\n+0610009E02Rik\t104.270660011093\t86.522462562396\t99.8336106489185\t99.2789794786467\t110.371602884082\t127.010537992235\t97.0604547975596\t90.9595119245702\t103.161397670549\n+0610009L18Rik\t45.2342487883683\t43.6187399030695\t24.232633279483\t32.3101777059774\t30.6946688206785\t32.3101777059774\t19.3861066235864\t25.8481421647819\t17.7705977382876\n+0610009O20Rik\t303.24459234609\t311.148086522463\t351.497504159734\t354.409317803661\t363.144758735441\t367.304492512479\t332.362728785358\t340.682196339434\t417.637271214642\n+0610010F05Rik\t134.902411021814\t113.2797550708\t142.365097588978\t110.218140068886\t136.050516647532\t131.8407960199\t140.642939150402\t135.859165709912\t145.426712590892\n+0610010K14Rik\t8.9662447257384\t4.21940928270042\t7.91139240506329\t11.0759493670886\t12.6582278481013\t15.295358649789\t9.49367088607595\t11.0759493670886\t10.5485232067511\n+0610012D04Rik\t3.76647834274953\t0\t1.88323917137476\t0\t1.88323917137476\t3.76647834274953\t5.64971751412429\t5.64971751412429\t0\n+0610012G03Rik\t343.252595155709\t362.629757785467\t416.608996539792\t274.048442906574\t332.179930795848\t305.882352941176\t318.339100346021\t395.155709342561\t382.698961937716\n+0610030E20Rik\t68.5871056241427\t57.9561042524006\t61.7283950617284\t60.6995884773663\t60.0137174211248\t69.9588477366255\t66.0150891632373\t62.5857338820302\t75.6172839506173\n+0610033M10Rik\t0\t0\t0\t1.81488203266788\t0\t0.907441016333938\t1.81488203266788\t0.907441016333938\t0.907441016333938\n+0610037L13Rik\t72.2527960234333\t75.0932007811113\t68.524764778981\t77.2235043493698\t71.7202201313687\t79.708858512338\t71.7202201313687\t89.6502751642109\t80.0639091070477\n+0610038B21Rik\t12.3738196027353\t8.46629762292413\t12.6994464343862\t21.1657440573103\t17.5838489091501\t24.7476392054705\t15.3044610875936\t15.3044610875936\t15.6300879192445\n+0610039K10Rik\t17.8571428571429\t15.7563025210084\t16.8067226890756\t22.0588235294118\t32.563025210084\t45.1680672268908\t35.7142857142857\t35.7142857142857\t33.6134453781513\n+0610040B10Rik\t4.76644423260248\t8.10295519542421\t7.14966634890372\t10.9628217349857\t5.24308865586273\t9.53288846520496\t8.10295519542421\t9.05624404194471\t4.76644423260248\n+0610040F04Rik\t12.5581395348837\t12.5581395348837\t11.1627906976744\t10.6976744186047\t15.3488372093023\t20.9302325581395\t13.4883720930233\t15.3488372093023\t14.4186046511628\n+0610040J01Rik\t9.15522263836871\t6.24219725343321\t9.15522263836871\t18.7265917602996\t20.3911776945485\t22.4719101123595\t20.8073241781107\t22.4719101123595\t21.6396171452351\n+0610043K17Rik\t9.09090909090909\t3.8961038961039\t14.2857142857143\t5.19480519480519\t2.5974025974026\t5.19480519480519\t7.79220779220779\t10.3896103896104\t5.19480519480519\n+1010001B22Rik\t10.1867572156197\t10.1867572156197\t11.8845500848896\t6.7911714770798\t13.5823429541596\t15.2801358234295\t6.7911714770798\t10.1867572156197\t8.48896434634975\n+1010001N08Rik\t0.970873786407767\t1.45631067961165\t1.94174757281553\t0.970873786407767\t0\t0\t1.94174757281553\t1.45631067961165\t1.94174757281553\n+1110002E22Rik\t0\t0\t0\t0.2000400080016\t0\t0\t0.1000200040008\t0\t0.1000200040008\n+1110002J07Rik\t33.4261838440111\t29.4468762435336\t36.6096299243932\t83.5654596100279\t79.5861520095503\t104.257859132511\t60.0875447672105\t56.9040986868285\t66.8523676880223\n+1110002L01Rik\t97.3129992737836\t88.5984023238925\t96.3447107237957\t81.5783103364803\t84.9673202614379\t99.0075042362624\t91.5032679738562\t97.5550714112806\t91.5032679738562\n+1110002O04Rik\t0\t0\t0\t1.76678445229682\t0\t1.76678445229682\t0\t0\t0\n+1110003F10Rik\t20.8604954367666\t6.51890482398957\t23.4680573663625\t14.3415906127771\t9.1264667535854\t9.1264667535854\t6.51890482398957\t10.4302477183833\t10.4302477183833\n+1110004E09Rik\t277.941176470588\t321.'..b'5217391304\t43.4782608695652\t26.0869565217391\t26.0869565217391\n+n-R5s188\t47.6190476190476\t9.52380952380952\t28.5714285714286\t95.2380952380952\t47.6190476190476\t28.5714285714286\t28.5714285714286\t19.047619047619\t19.047619047619\n+n-R5s19\t0\t0\t0\t0\t0\t0\t8.33333333333333\t0\t0\n+n-R5s191\t8.62068965517241\t0\t0\t0\t0\t0\t0\t0\t0\n+n-R5s192\t33.6134453781513\t8.40336134453782\t8.40336134453782\t0\t0\t8.40336134453782\t8.40336134453782\t0\t16.8067226890756\n+n-R5s194\t0\t8.40336134453782\t0\t0\t8.40336134453782\t0\t0\t0\t0\n+n-R5s196\t17.0940170940171\t42.7350427350427\t8.54700854700855\t0\t42.7350427350427\t8.54700854700855\t8.54700854700855\t17.0940170940171\t17.0940170940171\n+n-R5s197\t16.8067226890756\t0\t0\t25.210084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|
b |
diff -r 000000000000 -r ce3d027ec26b test-data/tpm.tab --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/tpm.tab Mon Jun 24 13:37:16 2019 -0400 |
b |
b'@@ -0,0 +1,35194 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|