| Previous changeset 2:791a47d9a777 (2018-12-09) Next changeset 4:39401213c7a2 (2018-12-09) |
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Commit message:
Deleted selected files |
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removed:
Project_RM/codon_usage.py Project_RM/codon_usage.xml |
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| diff -r 791a47d9a777 -r fb16cdb5f2e2 Project_RM/codon_usage.py --- a/Project_RM/codon_usage.py Sun Dec 09 06:08:47 2018 -0500 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 |
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| @@ -1,75 +0,0 @@ -#!/home/gianmarco/galaxy-python/python - -import Bio -from Bio import SeqIO -from Bio.Data import CodonTable -import re -import sys -import os -import pandas as pd - -def read_input(data = "example.fna"): - - seqs = "" - with open(data, "rU") as handle: - for record in SeqIO.parse(handle, "fasta"): - seqs = seqs + str(record.seq) - - return seqs - -def codon_usage(seqs, codonTable): - - codon_usage = {} - tmp = [x for x in re.split(r'(\w{3})', seqs) if x != ""] - - b_cod_table = CodonTable.unambiguous_dna_by_name[codonTable].forward_table - - - for cod in CodonTable.unambiguous_dna_by_name[codonTable].stop_codons: - b_cod_table[cod] = "_Stop" - - for cod in CodonTable.unambiguous_dna_by_name[codonTable].start_codons: - b_cod_table[cod + " Start"] = b_cod_table[cod] - b_cod_table.pop(cod) - - aas = set(b_cod_table.values()) - - - for aa in aas: - codon_usage[aa] = {} - for codon in b_cod_table.keys(): - if b_cod_table[codon] == aa: - codon_usage[aa][codon] = tmp.count(codon.split(" ")[0]) - - - tups = {(outerKey, innerKey): values for outerKey, innerDict in codon_usage.iteritems() for innerKey, values in innerDict.iteritems()} - - #aas_ = set(tups.keys()) - - #stops_ = {el for el in aas_ if el[0] == "Stop"} - #aas_ = list(aas_.difference(stops_)) - #stops_ = list(stops_) - #aas_.sort() - #stops_.sort() - - codon_usage_ = pd.DataFrame(pd.Series(tups), columns = ["Count"]) - codon_usage_.index = codon_usage_.index.set_names(["AA", "Codon"]) - #codon_usage_.index.reindex(pd.MultiIndex.from_tuples([aas_, stops_], names=('AA', 'Codon')), level=[0,1]) - - - codon_usage_['Proportion'] = codon_usage_.groupby(level=0).transform(lambda x: (x / x.sum()).round(2)) - - return {"Dictionary": codon_usage, "Tuples": tups, "Table": codon_usage_} - - - -if __name__ == '__main__': - - - seqs = read_input(data=sys.argv[1]) - out = codon_usage(seqs,"Bacterial") - - - with open(sys.argv[2], "w") as outf: - out["Table"].to_csv(outf, sep="\t") - #sys.stdout.write(out['Table']) \ No newline at end of file |
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| diff -r 791a47d9a777 -r fb16cdb5f2e2 Project_RM/codon_usage.xml --- a/Project_RM/codon_usage.xml Sun Dec 09 06:08:47 2018 -0500 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 |
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| @@ -1,44 +0,0 @@ -<tool id="codon_usage" name="Codon Usage" version="0.1.0"> - <description>for each sequence in a file</description> - <requirements> - <requirement type="python module">biopython</requirement> - <requirement type="python module">pandas</requirement> - </requirements> - - <command interpreter="/home/gianmarco/galaxy-python/python">codon_usage2.py -i $input -t $input_type -o $output -c $codon_table</command> - <inputs> - <param name="input" format="fasta" type="data" label="Source file"/> - - <param name="input_type" type="select" format="text"> - <label>Indicate the input file format</label> - <option value="fasta">Fasta</option> - <option value="gbk">gbk</option> - </param> - - <param name="codon_table" type="select" format="text"> - <label>Choose the proper codon table for your organism)</label> - <option value="Archaeal"> Archaeal</option> - <option value="Bacterial">Bacterial</option> - <option value="Standard">Standard</option> - - </param> - - </inputs> - - - - <outputs> - <data format="tabular" name="output" /> - </outputs> - - <tests> - <test> - <param name="input" value="fa_gc_content_input.fa"/> - <output name="out_file1" file="fa_gc_content_output.txt"/> - </test> - </tests> - - <help> -This tool compute codon usage of an annotated genome [preferably Prokaryotes]. - </help> -</tool> |