| Next changeset 1:a2c318b04f49 (2019-05-16) |
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Commit message:
planemo upload |
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added:
nanofilt.xml test-data/input.fastq test-data/output.fastq |
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| diff -r 000000000000 -r ff1cc05e789a nanofilt.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/nanofilt.xml Tue May 14 05:57:15 2019 -0400 |
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| @@ -0,0 +1,107 @@ +<tool id="nanofilt" name="NanoFilt" version="0.1.0"> + <description>Filtering and trimming of long read sequencing data</description> + <requirements> + <requirement type="package" version="2.3.0">nanofilt</requirement> + </requirements> + <command detect_errors="exit_code"><![CDATA[ + NanoFilt + #if str($filter.quality) != "" + -q ${filter.quality} + #end if + #if str($filter.minlength) != "" + --length ${filter.minlength} + #end if + #if str($filter.maxlength) != "" + --maxlength ${filter.maxlength} + #end if + #if str($crop.head) != "" + --headcrop ${crop.head} + #end if + #if str($crop.tail) != "" + --tailcrop ${crop.tail} + #end if + #if $summary.selector + --readtype "${summary.readtype}" + --summary "${summary.summary_file}" + #end if + --logfile $logfile + "$input1" > "$output1" + ]]></command> + <inputs> + <param type="data" name="input1" format="fastqsanger" label="uncompressed fastq file" /> + <section name="filter" title="Options for filtering reads based on length or quality"> + <param name="quality" type="integer" label="Filter on a minimum average read quality score" value="30" min="0" max="255" help="(-q)" optional="true" /> + <param name="maxlength" type="integer" label="Filter on a maximum read length" help="(--maxlength)" optional="true" /> + <param name="minlength" type="integer" label="Filter on a minimum read length" help="(--length)" optional="true" /> + </section> + <section name="crop" title="Options for trimming reads"> + <param name="head" type="integer" label="Trim n nucleotides from start of read" help="(--headcrop)" optional="true" /> + <param name="tail" type="integer" label="Trim n nucleotides from end of read" help="(--tailcrop)" optional="true" /> + </section> + <conditional name="summary"> + <param name="selector" type="boolean" checked="false" label="Use albacore or guppy summary file for quality scores"/> + <when value="true"> + <param type="data" name="summary_file" format="tabular" label="Summary file generated by albacore or guppy."/> + <param name="readtype" argument="--readtype" type="select" label="Which read type to extract information about from summary"> + <option value="1D" selected="true">1D</option> + <option value="2D">2D</option> + <option value="1D2">1D2</option> + </param> + </when> + <when value="false"> + <param name="minGC" type="float" label="Sequences must have GC content higher than this" min="0.0" max="1.0" help="(--minGC)" optional="true" /> + <param name="maxGC" type="float" label="Sequences must have GC content lower than this" min="0.0" max="1.0" help="(--maxGC)" optional="true" /> + </when> + </conditional> + </inputs> + <outputs> + <data name="output1" format="fastq" /> + <data name="logfile" format="tabular" label="Log file" from_work_dir="logfile.txt" /> + </outputs> + <tests> + <test> + <param name="input1" value="input.fastq"/> + <output name="output1" file="output.fastq"/> + </test> + </tests> + <help><![CDATA[ + usage: NanoFilt [-h] [-v] [--logfile LOGFILE] [-l LENGTH] [--maxlength MAXLENGTH] + [-q QUALITY] [--minGC MINGC] [--maxGC MAXGC] [--headcrop HEADCROP] [--tailcrop TAILCROP] + [-s SUMMARY] [--readtype {1D,2D,1D2}] + + Perform quality and/or length and/or GC filtering of (long read) fastq data. Reads on stdin. + + EXAMPLES: + + gunzip -c reads.fastq.gz | NanoFilt -q 10 -l 500 --headcrop 50 | minimap2 genome.fa - | samtools sort -O BAM -@24 -o alignment.bam - + + gunzip -c reads.fastq.gz | NanoFilt -q 12 --headcrop 75 | gzip > trimmed-reads.fastq.gz + + + ]]></help> + <citations> + <citation type="bibtex"> + @misc{githubnanofilt, + title = {nanofilt}, + publisher = {GitHub}, + journal = {GitHub repository}, + url = {https://github.com/wdecoster/nanofilt} + } + @article{10.1093/bioinformatics/bty149, + author = {De Coster, Wouter and D’Hert, Svenn and Schultz, Darrin T and Cruts, Marc and Van Broeckhoven, Christine}, + title = "{NanoPack: visualizing and processing long-read sequencing data}", + journal = {Bioinformatics}, + volume = {34}, + number = {15}, + pages = {2666-2669}, + year = {2018}, + month = {03}, + abstract = "{Here we describe NanoPack, a set of tools developed for visualization and processing of long-read sequencing data from Oxford Nanopore Technologies and Pacific Biosciences.The NanoPack tools are written in Python3 and released under the GNU GPL3.0 License. The source code can be found at https://github.com/wdecoster/nanopack, together with links to separate scripts and their documentation. The scripts are compatible with Linux, Mac OS and the MS Windows 10 subsystem for Linux and are available as a graphical user interface, a web service at http://nanoplot.bioinf.be and command line tools.Supplementary data are available at Bioinformatics online.}", + issn = {1367-4803}, + doi = {10.1093/bioinformatics/bty149}, + url = {https://doi.org/10.1093/bioinformatics/bty149}, + eprint = {http://oup.prod.sis.lan/bioinformatics/article-pdf/34/15/2666/25230836/bty149.pdf}, + } + </citation> + </citations> +</tool> |
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| diff -r 000000000000 -r ff1cc05e789a test-data/input.fastq --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/input.fastq Tue May 14 05:57:15 2019 -0400 |
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| b'@@ -0,0 +1,73000 @@\n+@NB501061:3:HF573AFXX:1:11101:3103:1113 1:N:0:CGTACTAG+NTAGAGAG\n+TTCCTATAACTGGTTGACTTGAACCAACAGTTGCTATTAAATTTGCTCGACCATCTGTAACTGTATCAATA\n++\n+AAAAAEEEEEAEEEEE6EEEEAAEEEEEEAEAEEEEEEEEAEEEEEEEEEEEEEEEAEEEEEEEEEEEAEE\n+@NB501061:3:HF573AFXX:1:11101:2518:1121 1:N:0:CGTACTAG+NTAGAGAG\n+GATTTACTTATGGACGATATGGATGGTGTAGAAGCAACTACTGAAATAAAAAAAGATTTACCTCAAATTAAAGTAGTCATGTTAACAAGCTTTATAGAGGATAAAGAAGTTTATCGTGCACTTGATTCTGGAGTAGATAGTTATATTTTAA\n++\n+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEAA6EEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEAEEAEEEEEAEEEEEEE/EEAEEEEEEEA<EEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEAAAE\n+@NB501061:3:HF573AFXX:1:11101:22477:1123 1:N:0:CGTACTAG+NTAGAGAG\n+GGTCTATTACGTAAAGGTGAAGGTGACATGGTCATGGAGCAATTCGGTGAAGGTTTTGATATGAATATTATTCGTGTTAATGCTCAAGAACGCTTCATGAGTAAACTAAAAGGTGTGTCTGATCCAGAACGTAAACGTAAAATCATTGGTA\n++\n+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEE/EEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEAEEEEEEAEEEEEEE/EE/EEEE/EEEEEEE/AEEEEEEEEEEEEEEAEEEEEEEEEEEEEEAEEEEE<AEEEAEEEEEEEEEEAE\n+@NB501061:3:HF573AFXX:1:11101:24857:1142 1:N:0:CGTACTAG+NTAGAGAG\n+GTGCTGTATATCTTGTAAATCTATTCTCTAATATTAAAACTCCAGAGAATCTTAAACATATCAAAGAGCCATACGATGAGCACACAGATATCCACTTGATGAAAGCGATAAGCGAAAGTGACACAGTCATTCTAGCTTATGGCGCCTATG\n++\n+AAAAAEEEEEEEEEEEEEEEEEEEEAEEEEEAEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEE/E<AAEEEEEEA/<<AAAAEEAEEEEEAEAAEEAE/EEEAA/EAEEEE/EEAEA/E<A<A<EA<<EA<E<EE/EEE<EEAE//E\n+@NB501061:3:HF573AFXX:1:11101:12352:1144 1:N:0:CGTACTAG+ATAGAGAG\n+TAGCTGGCTTAAACCAAAACGATCAGCCTCTTCTATGATTAAAGTATTAGCATTTGGTACATCTACACCTGTTTCAATAATTGTAGTCGTTACTAAAATATCGTACTCGTGATTAATAAAGCTTAACATTGTTTCCTCTAAATCACGTTCA\n++\n+AAAAAEEEEEEA6EEEE6EEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEAEEEEEEEEEEEEEEEEEEEEEE6EEEEEAEEEEEEEEEEEEEAEEEE/EEEEEEEEEEEEEEEEEEEEEEEEEEAEEEA\n+@NB501061:3:HF573AFXX:1:11101:15400:1147 1:N:0:CGTACTAG+ATAGAGAG\n+TATGTACAGTATGACAGTATTTAGTTTAATGAACTTTTGTCTATTGTCATTTCCATTAGATTTGATTGAAGAGCGAAATCAAGGTTGGTATAAGCGGTTAATGGTGACCCCATTATCATCATTTCAATATTATTTAGTTAAAATTAGTAAG\n++\n+AAAAAEEEEEEEEEEEEEEEEEE6EEEE<EAEAEEEEEEEEEEEEEEEEEEEE/EEEAEEEEEEEAEEEEAEE66EEEEEEEEEEEE/EEEEEAA<EEEEEEEEEEA/<AEEE/EE<E<<E/EEEEE/EEEE<EAEEEEEEEEEEAEEAE/\n+@NB501061:3:HF573AFXX:1:11101:2858:1149 1:N:0:CGTACTAG+ATAGAGAG\n+ACTAAAGATGAGCGTTTAGATGCAGTGATGAGTGCAGGAGGACTGCAAGAATGTGGTAATTCTCAAAATAGTGTGAATGCATGTCCTAAAGGTATACCATTGACAACGTCAAATGCCGCAATGAATAGAGAAACTTCTTTCCATATGTTAA\n++\n+/AAA66E//EE6EE/AEEEEE<///EA//EEE/E/EEEAAE//EEAA/A6E/6A//E/EEE<EAEEEEE//EE/E/EEEEA</EEE/EAEE/AE/EEEEE/EAEE//<AEE///</<<E<E/EEE/AA/<AAAA/EA/E/A<EEAAEE///\n+@NB501061:3:HF573AFXX:1:11101:4491:1150 1:N:0:CGTACTAG+ATAGAGAG\n+ATGCATTGTAATCGAAACATACCCCTTGTATAGACTTCATCGGATCGATTAACACCTATCACTTGAGTCACAGCACTTGGAATAGCATTCATAGATAAAATAACCCCTAGTGCTACGACAGGATATATTTGTTGATAAGCATATAAACCGT\n++\n+<AAAAEEEEEE6AE6EE6EEE/EEEEEEEEEEE6EEEE/EEEEEEEEEE6EEE6EEEEAEE6EEE/EEE6E6E6EAEEEE6EA<EEAEAEE/AAEEAEAEEEEAEEEE/AEEAAEEEEEEEEEEEEEEEEAEE/A<EEEEEEE</E/AEE/\n+@NB501061:3:HF573AFXX:1:11101:12967:1151 1:N:0:CGTACTAG+ATAGAGAG\n+TAGTAAGACTATACGGTTTGTCTTTTTAGAAAATTCTGATAGAATAAAGTTATATCCGATAGAAAAACTAAGTATTGATAAATTGTAAAACTAAAAGGAGTGTTTTGGATGGCACAAAAACCTGTAGATTATGTTACACAAATTATTGGGA\n++\n+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEE<AEEAEEEEEEEEAEEEEEEEEEEEEEEEEEEEEAEEEEEEE<EEEEEEEEEEEEEEEEEEAEEEAEEEEEE\n+@NB501061:3:HF573AFXX:1:11101:24302:1152 1:N:0:CGTACTAG+ATAGAGAG\n+ATTGTATAATTATAAAGAAATAAGAACGCCAATTTTTGAAAGTACTGAACTTTTTGCAAGAGGCGTGGGGGATTCTACTGAGGTTGATCAAAAGGAAATGTATACATTTAAAGATAAAGGGGATCGTAGTTTAACATTACGTCCTGAAGGA\n++\n+/AA/A///EA/AEEE6A//6EEE6E6A/////E/EEEEEEEE/EE/EE6/EA/A</E/E/E//E////AEE//<6A///////EEA///E//E//E/EA//A//AEE/E//E6EEAA///E/AAA</E///A/E/EE//</E666<//EE/\n+@NB501061:3:HF573AFXX:1:11101:6728:1157 1:N:0:CGTACTAG+ATAGAGAG\n+TACTAAAAGGTAGCGATATTTTAGGAACGAATAACGCGCTTAGTCACGCTTTTAGTGGTTTTTCAACCTCAGCCGTAGCACTTGTAGCTGCAGCATTATTTTTAGCAGTAGCTATGCAGGAAACCAATTTACATAAACGACTTGCATTA\n++\n+AAAAAEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEE/EEEEEEEEEEEEEEEEEEE/EEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEE<EEEEEEEEEEE/A6AEEEEEE\n+@NB501061:3:HF573AFXX:1:'..b'NB501061:3:HF573AFXX:1:11106:25404:2014 1:N:0:CGTACTAG+ATAGAGAG\n+TCCGACGATATCTTCTTCTGATAAACCTTCCAAATACTTAATTAATGCGCGAATGGAATTTCCGTGGGCCGAAACAAGTACAGTTTTATCATCTAATAGGTGTTGTGAAATATGATCAGTCCAGAACGGAACAACTCGTTCTAATGTAT\n++\n+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEAEEEEEEAEEEAEEEE/A/EEEEEEEEEEEEEAAE\n+@NB501061:3:HF573AFXX:1:11106:20809:2016 1:N:0:CGTACTAG+ATAGAGAG\n+CTTTATTAATATGTAAAAATAAGTGTTTTATTTGCACGCTAACTTGGCTTTTCAACATAAATACTTTTTGAGTAAAAAGGAGGAACTTTTATGGATTATGCACATTTAAATCTCGAGCACTTTTTTGCAAGAAATGATGATTTGGATATTA\n++\n+AAAAAEEEEEEEEEEEEEEEE6EEEAAEEEEEEAEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE6EEEEEEEEEEEEEEEEEEEEEEEE/EEEEEEEEEEEEEEEAEEE6EEEEEEAEEEEEEEE<EEEE/\n+@NB501061:3:HF573AFXX:1:11106:2456:2024 1:N:0:CCTACTAG+ATAGAGAG\n+GAATATCGAGAGAGTGAATATTGCTTCGCACTTATCAAAGTAGTAACAGTCATCATATTAATAGCAAATGGAATCTTAACTATTTTACGCATTATGGGTGGAGAATTTGAAGCAAATGATACCTTTAGAAAAGGAGAAGGCCCAATAGAAG\n++\n+A6/AAA/EEEE6EE6E6E/AEAE/EE/EEE///EEEA//E/6EE/EE66E/A/EAE6/E6EEEA/EE///E/E/EAEE///EEE//E/E/E//A///EAE<E/EE//EE//A/E///A///</E//A//6<EAEEE///E/6///////<A\n+@NB501061:3:HF573AFXX:1:11106:15329:2025 1:N:0:CGTACTAG+ATAGAGAG\n+CCTTATACAACTTCACAAACTCTTCTTCATTCATGGCAAGCTTTGGAAATAACATATTATATTTTTTTTCGATATCTTTCTGTAACTTCACTACTTTAGGCAACTCTATATGCCACTTTAATAATTGCGTTTTTTGATTAAATAAATTT\n++\n+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE6E/EEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEE<EEEEEEEEEEEAAEE/EEEEA\n+@NB501061:3:HF573AFXX:1:11106:20334:2026 1:N:0:CGTACTAG+ATAGAGAG\n+CTATAATGCCAAAGATCCACAAAAACATGCACTGTTTCCTAAATATGATTTCTTTAGAGCAGATACTGACTATGCTGACATTGCAAAATTTTTAGGACTCAAAGGTAATACAACTGAAGAATTAGTGGATGCTCTAGCTAATGCGGTGTAT\n++\n+AAAAAEEEEAEEEEEEEEEEEEEE6EEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE/AEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEAEEEEEEAEEEEEEEAEEEEE/EEEEEE<EEEAE/EEEE<AA<EEEE\n+@NB501061:3:HF573AFXX:1:11106:2427:2031 1:N:0:CGTACTAG+ATAGAGAG\n+ACATCCGATAGTGCATCTACATCAAAGAGTGTAAGTGAGTCAAACAGCGCCAGTACATCGGTAAGTGGCTCACCAAGTACAAGCATTTCAGATTCATCAAGCACGTCCACATCAATGAGTACATCTGAAACTTTCACTTCTCAATCTCCTA\n++\n+6AAAAEEAEEEAEEEEEEEE6EEEEEEEEEEEEAEEEEAAEEEEA/EEAEEEEE/EEEEEEEEEEEEEEEEE/<E/AEEAAEEAEEAEEEE/EAEEEA/<<EEE<EEEEAEAAA/E<EA</AEEEEA/EE///AE//E<EEE///<E6AA/\n+@NB501061:3:HF573AFXX:1:11106:10496:2032 1:N:0:CGTACTAG+ATAGAGAG\n+CCATTAGGTCGACTAGGAACACCGGATGAAGTCGGGAAACTTGTAGCATTTTTAGCTTCCGATGATAGTTCATTTATAACTGGTGAAACTATTCGTATAGATGGTGGCGTTATGGCTTATACATGGCCTGGAGAAATGTTAAGTGATGAAA\n++\n+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEAEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEE6EEEEE/EEEEEEEE/\n+@NB501061:3:HF573AFXX:1:11106:15681:2048 1:N:0:CGTACTAG+ATAGAGAG\n+ATATATGACTGCTGAAGAAACAAAACAATTTAATGAAGGTGTATCAGGTGATTTCGTTGGCATAGGTGCTGAAATGCAAAAGAAAAATGAACAGATAAGTGTTACTAGTCCAATGAAGGATTCACCAGCAGAAAAAGCTGGTATTCAAC\n++\n+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEE6EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEAEEEEEE<EEEEEEEEEEEEAEEEEEAEEEE/<E\n+@NB501061:3:HF573AFXX:1:11106:11089:2054 1:N:0:CGTACTAG+ATAGAGAG\n+CTAAAGGACTAAATGCAGCTCCAAAACCAGCCCAAGCATTACCAACTAAATTTAGTATTGTATCATTTGGATGCCAAGCAATCGTAATAGCAACTATCGCAACAAGTAAAACTGATAAGCGTCCAATCAAAACAAACTCTTTTTGGTGTGA\n++\n+AAAAAEEEEEEEEEEEEEEEEEEEE6EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEA6EAEEEE/EAEEEEEEEEEEEEEE/AEEE/EEEEEE/EE//EEAE//EEE6EEE<AEAEEE<EEE/\n+@NB501061:3:HF573AFXX:1:11106:18404:2057 1:N:0:CGTACTAG+ATAGAGAG\n+TTATAAGACACATACAATTTTTAATATATCGATTAACTAAAGGTGAGTATTTAGAAGCACAGGAGAATTTTATATCGATGATAAAAACTATGTATC\n++\n+AAAAAEEEEEEEEAEEEEEEAEEEEEEEAA<EEEEEEE/EEEAEEEEEEEEEEEEEA<E<EEAEEEEEEEEEEEE//EEAEEEEEEEEAEEEEEE<\n+@NB501061:3:HF573AFXX:1:11106:22800:2060 1:N:0:CGTACTAG+ATAGAGAG\n+CAAGTATATCATGCTAAAGTAAAACGTATTGAAAAGTATGGTGCTTTCGTTGAATTGTTCCCTGGTAAAGACGCGTTATTACACATTTCTCAAATTTCACAAGAAAGAATTAATAAAGTAGAAGATGTTCTTAAAATTTGAGATACAATTG\n++\n+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEE/E/<EAEEEEEAEEEEEAEEEEEEEEEAEEEEEEAEE/EEEAEEEEEEEE/EE/EE/EEEAEEEEEEEE\n' |
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| diff -r 000000000000 -r ff1cc05e789a test-data/output.fastq --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/output.fastq Tue May 14 05:57:15 2019 -0400 |
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| b'@@ -0,0 +1,27872 @@\n+@NB501061:3:HF573AFXX:1:11101:3103:1113 1:N:0:CGTACTAG+NTAGAGAG\n+TTCCTATAACTGGTTGACTTGAACCAACAGTTGCTATTAAATTTGCTCGACCATCTGTAACTGTATCAATA\n++\n+AAAAAEEEEEAEEEEE6EEEEAAEEEEEEAEAEEEEEEEEAEEEEEEEEEEEEEEEAEEEEEEEEEEEAEE\n+@NB501061:3:HF573AFXX:1:11101:2518:1121 1:N:0:CGTACTAG+NTAGAGAG\n+GATTTACTTATGGACGATATGGATGGTGTAGAAGCAACTACTGAAATAAAAAAAGATTTACCTCAAATTAAAGTAGTCATGTTAACAAGCTTTATAGAGGATAAAGAAGTTTATCGTGCACTTGATTCTGGAGTAGATAGTTATATTTTAA\n++\n+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEAA6EEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEAEEAEEEEEAEEEEEEE/EEAEEEEEEEA<EEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEAAAE\n+@NB501061:3:HF573AFXX:1:11101:12352:1144 1:N:0:CGTACTAG+ATAGAGAG\n+TAGCTGGCTTAAACCAAAACGATCAGCCTCTTCTATGATTAAAGTATTAGCATTTGGTACATCTACACCTGTTTCAATAATTGTAGTCGTTACTAAAATATCGTACTCGTGATTAATAAAGCTTAACATTGTTTCCTCTAAATCACGTTCA\n++\n+AAAAAEEEEEEA6EEEE6EEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEAEEEEEEEEEEEEEEEEEEEEEE6EEEEEAEEEEEEEEEEEEEAEEEE/EEEEEEEEEEEEEEEEEEEEEEEEEEAEEEA\n+@NB501061:3:HF573AFXX:1:11101:12967:1151 1:N:0:CGTACTAG+ATAGAGAG\n+TAGTAAGACTATACGGTTTGTCTTTTTAGAAAATTCTGATAGAATAAAGTTATATCCGATAGAAAAACTAAGTATTGATAAATTGTAAAACTAAAAGGAGTGTTTTGGATGGCACAAAAACCTGTAGATTATGTTACACAAATTATTGGGA\n++\n+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEE<AEEAEEEEEEEEAEEEEEEEEEEEEEEEEEEEEAEEEEEEE<EEEEEEEEEEEEEEEEEEAEEEAEEEEEE\n+@NB501061:3:HF573AFXX:1:11101:9265:1161 1:N:0:CGTACTAG+ATAGAGAG\n+CAATTAAATGACCGCCGTCATCATCTGGTAATCGGTCTTCTCTTCCTACTGTTCGTTGCGCATATGAATTACGTCCGCCATCTAGAAGTGATAGGTCATCCGCATATACTTCTTTAATACGGCCTTTGTGATC\n++\n+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE/EEEEEEEEEEEEEE</EEEEEEEEEEEEEEEE<EEEAEEEEAEEEEEEEAAAEEE<EEEEEEEEE\n+@NB501061:3:HF573AFXX:1:11101:15298:1176 1:N:0:CGTACTAG+ATAGAGAG\n+GCTCATCATTGTACTGGATTTTTTAAAACTACATTGCCTGGCTTTTATAGTGTAGTTAAAGATATTTTTACACCGAACTATGAAAGGAAAGAGAGTTAGAATATGTTGCAACCTTTGATACATGGACTATTATTAGCTTTAGGTTTAATTT\n++\n+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEE<EEEEEEE/EEEEEEEEEEAEEEEEEEEEEEEEE<AEEEEEEEEAEEEE\n+@NB501061:3:HF573AFXX:1:11101:21083:1190 1:N:0:CGTACTAG+ATAGAGAG\n+CTTTTACTTATTCTAATTTACATTGATTAAAACACCGATTTTATTCTATCTTTAATTTAGCCATGATGTATTACCTCTTAGCTATAGCTTTAATTAGATGGCATTTCATCCTCAAACTCATCTCAATTGATTATGTTTGTTGAATTAAAAA\n++\n+AAAAAEEEEEEEEEEEEEEEEAEEEEEEEEEAEEEAEAEEEEAEEEEEEEEEEEEEEEEEEEAEEEEEEAEEEEAEEEAEEAA<EEEEEEAEEEEAE/EEEEEEEEEEEE6AEEEEEEAAEEE<EEEE<<EEAEAAEEEEEEAAEE/AAE6\n+@NB501061:3:HF573AFXX:1:11101:21687:1270 1:N:0:CGTACTAG+ATAGAGAG\n+CAGTAAGCATGATGATAGGTGTATCTTTATGTTCTCTAAGTTTAGAGGCAACTTCAATTCCATCCATTTCAGGAAGCATTAAATCTAAGAGGATGCACGCATAGTTGTTTTCAAGAGCAAGCTCGTAGGCTTCTTTACCATCTCTTGCTT\n++\n+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEEEEEEEEEEEEEEE/EAEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEE\n+@NB501061:3:HF573AFXX:1:11101:6075:1283 1:N:0:CGTACTAG+ATAGAGAG\n+ACTTCCCACTTGCTTCAGGTATACTAGCTGGCAAGTACGATGAAAATCAAACATTTGAGGATCATCGTGCAGAACGTCCTGATTTTCAACCAGACGTATTCAAAGATAATGTGCGTCGTGTAAAAGCATTAACTGATATTGCAGAGGCACA\n++\n+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE<EEEEEE/<EEEAE<EEE<EEEEEEEEEEEEEEAEEE\n+@NB501061:3:HF573AFXX:1:11101:3339:1291 1:N:0:CGTACTAG+ATAGAGAG\n+ATTTCTGAATGATGATTTTATATTACCATCGAAAATAGATATTTGTGTTGAGCGCAAAAAGTTATTGGAAATTATCAGGGTGATTCCTGAAGAATTTACGATTCACTATTATGATAATCAGTTATATGAAAGATTAAGAGAATCTTTAT\n++\n+AAAAAEEAEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEAAEE<EEEEEEEEEEEEAEEEEAE<EEEEEEEAEEEEEEEEEEAEEEAEEEEE<EEEE/EA6EEEEE\n+@NB501061:3:HF573AFXX:1:11101:22451:1312 1:N:0:CGTACTAG+ATAGAGAG\n+AATGTGATGTTAGTAGATTTAAATGAAAATAATCAAATTCAAGCTGAAAATGTGATTTTTATAGGCTTAATATGCAAAAATGCTGTTTGGCATGCTGTTGTATTTGAAATCGGTAGAGGTTACACTCGTGAATTATCTATCCTGGATATTA\n++\n+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEE<EEEEEEEEEEEEEEAAEEEEEEEEAEEA66AE/EEEEE/\n+@NB501061:3:HF573AFXX:1:11101:16981:1322 1:N:0:CGTACTAG+ATAG'..b'EEEEEEEEEEEEEEEEEEEEEEAEEAEEEEEEEEAEEEEEEEEEEEEEEEE\n+@NB501061:3:HF573AFXX:1:11106:20291:1959 1:N:0:CGTACTAG+ATAGAGAG\n+ATTTAAAGAATTATGGTTACCAATCATAACAATTTGTTTCATCTTAGCAATTTCAAAAATCACAACATATGGTGGTTTAAGTAATGCTATGGGACAAGGCATCTCAAAAGCAGGAAGCGTATTCCCAATATTATCACCAATCCTTGGTTGG\n++\n+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEAEEEEEEAEEEEEEEEEEEEEEEEAEAAEEEEEEEEEEEEE\n+@NB501061:3:HF573AFXX:1:11106:20230:1964 1:N:0:CGTACTAG+ATAGAGAG\n+CAGCAGTAGGTGGTTGGAAAATCATCAAAACTGTTGGCGGAAATATTATGAAAATTAGACCTGCTAACGGTGCAGCCGCTGATATTTCTTCAGCATTAACGATTTTCGTAGCGTCATCATTACATTTCCCATTATCTACTACACACGTTGT\n++\n+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE6EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEE<EEEEEEEEEAAEEEAEEEEEEE\n+@NB501061:3:HF573AFXX:1:11106:18598:1965 1:N:0:CGTACTAG+ATAGAGAG\n+GCTTAAGTACGGTTGGTACAATTAAAGATGCTGACAACTGGACTTTAGATAGTGGATTCTACAAAACGCCTTCTAAATACAGCTTAGGTGATTATGTATGGTACGATTCTAATAAAGATGGTTACCAAGACAATGATGAAAAAGGTATTAA\n++\n+AAAAAEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEE6EEEEEEEEEEEEEEEEEEEEEEE/EEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEAEEEEEEEEEE6AAEEEAEEAEEEEEAEEEEEEEEEEE\n+@NB501061:3:HF573AFXX:1:11106:17916:1985 1:N:0:CGTACTAG+ATAGAGAG\n+TCCCAAGCAGGCGTTAAAATACAACTCATCATTCGTGGTATATGTTGTCTTAAACCAGGCATTCCAGGTATTAGCGAAAATATAGAGGTTGTTAGTATAGTGGGTCGTTTGCTTGAACATTCACGTATTTACTACTTCCATAATAATAGTG\n++\n+AAAAAAEEEEEEEEEEEEEEEEE6EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEAEEAEEE<EEEEEAA<EE/EEEEEEE<EEEEEEEEEEEEEAEEEEEE\n+@NB501061:3:HF573AFXX:1:11106:25172:1992 1:N:0:CGTACTAG+ATAGAGAG\n+TTTTATTAATGAATTCATTGAGTGTTTGACTTTTATTAAAATAAGTTTCTAAATTTCTTATATAAAAC\n++\n+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE\n+@NB501061:3:HF573AFXX:1:11106:11380:2005 1:N:0:CGTACTAG+ATAGAGAG\n+GACCAGTTAAATATGCAACTAATGTTGCAGTAGAAATAACCGCAGCTAGTGTACCCCAGTCACGGAACAATGTCACCATAACCATGCTGATAATTGCATTAAATGCAATGGCAACACGTGGGATATTATACTTTTTATTAATTTTACCTAA\n++\n+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEA6EEE\n+@NB501061:3:HF573AFXX:1:11106:25404:2014 1:N:0:CGTACTAG+ATAGAGAG\n+TCCGACGATATCTTCTTCTGATAAACCTTCCAAATACTTAATTAATGCGCGAATGGAATTTCCGTGGGCCGAAACAAGTACAGTTTTATCATCTAATAGGTGTTGTGAAATATGATCAGTCCAGAACGGAACAACTCGTTCTAATGTAT\n++\n+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEAEEEEEEAEEEAEEEE/A/EEEEEEEEEEEEEAAE\n+@NB501061:3:HF573AFXX:1:11106:20809:2016 1:N:0:CGTACTAG+ATAGAGAG\n+CTTTATTAATATGTAAAAATAAGTGTTTTATTTGCACGCTAACTTGGCTTTTCAACATAAATACTTTTTGAGTAAAAAGGAGGAACTTTTATGGATTATGCACATTTAAATCTCGAGCACTTTTTTGCAAGAAATGATGATTTGGATATTA\n++\n+AAAAAEEEEEEEEEEEEEEEE6EEEAAEEEEEEAEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE6EEEEEEEEEEEEEEEEEEEEEEEE/EEEEEEEEEEEEEEEAEEE6EEEEEEAEEEEEEEE<EEEE/\n+@NB501061:3:HF573AFXX:1:11106:15329:2025 1:N:0:CGTACTAG+ATAGAGAG\n+CCTTATACAACTTCACAAACTCTTCTTCATTCATGGCAAGCTTTGGAAATAACATATTATATTTTTTTTCGATATCTTTCTGTAACTTCACTACTTTAGGCAACTCTATATGCCACTTTAATAATTGCGTTTTTTGATTAAATAAATTT\n++\n+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE6E/EEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEE<EEEEEEEEEEEAAEE/EEEEA\n+@NB501061:3:HF573AFXX:1:11106:10496:2032 1:N:0:CGTACTAG+ATAGAGAG\n+CCATTAGGTCGACTAGGAACACCGGATGAAGTCGGGAAACTTGTAGCATTTTTAGCTTCCGATGATAGTTCATTTATAACTGGTGAAACTATTCGTATAGATGGTGGCGTTATGGCTTATACATGGCCTGGAGAAATGTTAAGTGATGAAA\n++\n+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEAEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEE6EEEEE/EEEEEEEE/\n+@NB501061:3:HF573AFXX:1:11106:15681:2048 1:N:0:CGTACTAG+ATAGAGAG\n+ATATATGACTGCTGAAGAAACAAAACAATTTAATGAAGGTGTATCAGGTGATTTCGTTGGCATAGGTGCTGAAATGCAAAAGAAAAATGAACAGATAAGTGTTACTAGTCCAATGAAGGATTCACCAGCAGAAAAAGCTGGTATTCAAC\n++\n+AAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEE6EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEAEEEEEE<EEEEEEEEEEEEAEEEEEAEEEE/<E\n' |