| Next changeset 1:b820ef95ca20 (2024-05-14) |
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Commit message:
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastk commit 57fa4dcee2aaa115df4a5b7173a3a8fb889bc5a3 |
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added:
fastk.xml macros.xml test-data/input01.fasta.gz test-data/test02.tabex.txt test-data/test03.tabex.txt |
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| diff -r 000000000000 -r ff827f983bbb fastk.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/fastk.xml Fri May 03 20:23:04 2024 +0000 |
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| @@ -0,0 +1,114 @@ +<tool id="fastk_fastk" name="FastK" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="23.2"> + <description>A k-mer counter for high-quality assembly datasets</description> + <macros> + <import>macros.xml</import> + </macros> + <expand macro="requirements" /> + <command detect_errors="exit_code"><![CDATA[ + mkdir -p outfiles/tmpfiles && + #if $infile.is_of_type("fastq"): + #set INPUTFILE="input.fastq" + #elif $infile.is_of_type("fastq.gz"): + #set INPUTFILE="input.fastq.gz" + #else + #set INPUTFILE="input."+$infile.ext + #end if + ln -s '$infile' $INPUTFILE && + FastK $INPUTFILE + -k$kmer_size + #if $sorted_table.sorted_table_option == 'yes_with_default': + -t + #elif $sorted_table.sorted_table_option == 'yes_with_custom': + -t$sorted_table_cutoff + #end if + -T\${GALAXY_SLOTS:-8} -Noutfiles/output -Poutfiles/tmpfiles + #if $sorted_table.sorted_table_option == 'yes_with_default': + && Tabex outfiles/output.ktab -t${sorted_table.tabex_threshold_for_default} LIST > '$tabex_hist' + #elif $sorted_table.sorted_table_option == 'yes_with_custom': + && Tabex outfiles/output.ktab -t${sorted_table.tabex_threshold_for_custom} LIST > '$tabex_hist' + #end if + && tar -c -f fastk.tar ./outfiles/ + ]]></command> + <inputs> + <param name="infile" type="data" format="fasta,fasta.gz,fastq,fastq.gz,cram,unsorted.bam,sam" label="Input file"/> + <param name="kmer_size" argument="-k" type="integer" min="5" max="50" value="40" label="Enter desired k-mer size" help="Default: 40" /> + <conditional name="sorted_table"> + <param name="sorted_table_option" type="select" label="Sort table" help="Do you want a sorted table of all canonical k-mers and their counts? The sorted table is sorted lexicographically on the k-mer where a < c < g < t."> + <option value="no">No</option> + <option value="yes_with_default">Yes, Default sorted </option> + <option value="yes_with_custom">Yes, Custom sorted </option> + </param> + <when value="no"/> + <when value="yes_with_default"> + <param name="tabex_threshold_for_default" argument="-t" type="integer" value="5" min="1" label="Tabex count threshold" help="Trim all k-mers with counts less than threshold"/> + </when> + <when value="yes_with_custom"> + <param name="sorted_table_cutoff" type="integer" min="2" value="10" label="Enter sorted table cutoff value"/> + <param name="tabex_threshold_for_custom" argument="-t" type="integer" value="5" min="1" label="Tabex count threshold" help="Trim all k-mers with counts less than threshold"/> + </when> + </conditional> + </inputs> + <outputs> + <data name="fastk_out" format="tar" from_work_dir="fastk.tar" label="${tool.name} on ${on_string}: FastK files"/> + <data name="fastk_hist_out" format="fastk_hist" from_work_dir="outfiles/output.hist" label="${tool.name} on ${on_string}: FastK hist" /> + <data name="tabex_hist" format="txt" label="${tool.name} on ${on_string}: Tabex output"> + <filter> sorted_table['sorted_table_option'] != 'no' </filter> + </data> + </outputs> + <tests> + <!-- TEST 1 --> + <test expect_num_outputs="2"> + <param name="infile" value="input01.fasta.gz"/> + <output name="fastk_out" ftype="tar"> + <assert_contents> + <has_archive_member path="./outfiles/output.hist"/> + </assert_contents> + </output> + </test> + <!-- TEST 2 --> + <test expect_num_outputs="3"> + <param name="infile" value="input01.fasta.gz"/> + <conditional name="sorted_table"> + <param name="sorted_table_option" value="yes_with_default"/> + </conditional> + <output name="fastk_out" ftype="tar"> + <assert_contents> + <has_archive_member path="./outfiles/output.hist"/> + <has_archive_member path="./outfiles/output.ktab"/> + </assert_contents> + </output> + <output name="tabex_hist" value="test02.tabex.txt"/> + </test> + <!-- TEST 3 --> + <test expect_num_outputs="3"> + <param name="infile" value="input01.fasta.gz"/> + <conditional name="sorted_table"> + <param name="sorted_table_option" value="yes_with_custom"/> + <param name="sorted_table_cutoff" value="5"/> + </conditional> + <output name="fastk_out" ftype="tar"> + <assert_contents> + <has_archive_member path="./outfiles/output.hist"/> + <has_archive_member path="./outfiles/output.ktab"/> + </assert_contents> + </output> + <output name="tabex_hist" value="test03.tabex.txt"/> + </test> + </tests> + <help><![CDATA[ + FastK is a k‑mer counter that is optimized for processing high quality DNA assembly data sets such as those produced with an Illumina instrument or a PacBio run in HiFi mode. + + The input data can be in CRAM, BAM, SAM, fasta, or fastq files. + + FastK produces the following outputs: + + 1. FastK hist: file in binary format containing histogram information detailing the frequency of occurrence for each k‑mer within the dataset. + + 2. A Tabex txt file comprising a table of k‑mer/count pairs, sorted lexicographically on the k‑mer sequence, followingthe order a < c < g < t + + 3. A tar file comprising of hidden .ktab files that can be used for downstream FASTK tools. + + + ]]></help> + <expand macro="citations"/> +</tool> \ No newline at end of file |
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| diff -r 000000000000 -r ff827f983bbb macros.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/macros.xml Fri May 03 20:23:04 2024 +0000 |
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| @@ -0,0 +1,23 @@ +<macros> + <token name="@TOOL_VERSION@">1.0.0</token> + <token name="@VERSION_SUFFIX@">0</token> + <xml name="requirements"> + <requirements> + <requirement type="package" version="@TOOL_VERSION@">fastk</requirement> + </requirements> + </xml> + <xml name="citations"> + <citations> + <citation type="bibtex"> + @misc{github, + author = {Gene Meyers}, + year = {2020}, + title = {FastK}, + publisher = {GitHub}, + journal = {GitHub repository}, + url = {https://github.com/thegenemyers/FASTK}, + } + </citation> + </citations> + </xml> +</macros> \ No newline at end of file |
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| diff -r 000000000000 -r ff827f983bbb test-data/input01.fasta.gz |
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| Binary file test-data/input01.fasta.gz has changed |
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| diff -r 000000000000 -r ff827f983bbb test-data/test02.tabex.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/test02.tabex.txt Fri May 03 20:23:04 2024 +0000 |
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| b'@@ -0,0 +1,311 @@\n+Opening 40-mer table with 502,597 entries\n+ 1348: aaaaacgaaaccgttgttgaaatccctggaaagctggttg = 5\n+ 3497: aaaacatcttctgcaattccacgaaggcgctctcccattc = 6\n+ 4142: aaaacgaaaccgttgttgaaatccctggaaagctggttgc = 5\n+ 8271: aaaattagccaccatgccgcgcatctcacacccgcgcatg = 5\n+ 9568: aaacaggagaaagactatccccagtaacatcatgcccgta = 5\n+ 9898: aaacatcttctgcaattccacgaaggcgctctcccattcg = 6\n+ 11316: aaaccgttgttgaaatccctggaaagctggttgcgtgaaa = 5\n+ 11609: aaacgaaaccgttgttgaaatccctggaaagctggttgcg = 5\n+ 14677: aaagactatccccagtaacatcatgcccgtattccctgcc = 5\n+ 23585: aaattagccaccatgccgcgcatctcacacccgcgcatgc = 5\n+ 25526: aacaacggtttcgtttttcgctgacgttcagcaagccgct = 5\n+ 27763: aacaggagaaagactatccccagtaacatcatgcccgtat = 5\n+ 28631: aacatcatgcccgtattccctgccagctttaacacgtccc = 6\n+ 30223: aaccagctttccagggatttcaacaacggtttcgtttttc = 5\n+ 30777: aacccaccacggatatccgtaaggacagatgcagcaagtg = 5\n+ 32167: aaccggaaagacacgacaaagcgccactggcagcagccat = 5\n+ 32813: aaccgttgttgaaatccctggaaagctggttgcgtgaaaa = 5\n+ 33721: aacgaaaccgttgttgaaatccctggaaagctggttgcgt = 5\n+ 34290: aacgagcgggtcatgcagttgagtcagcaggtgcagcact = 5\n+ 35527: aacgcgatgctgaacgagcgggtcatgcagttgagtcagc = 7\n+ 36570: aacggcatctttagtttgcacgataaccatatcttcaatt = 5\n+ 36923: aacgggacaacgcgatgctgaacgagcgggtcatgcagtt = 7\n+ 37484: aacggtttcgtttttcgctgacgttcagcaagccgctgct = 5\n+ 37751: aacgtcagcgaaaaacgaaaccgttgttgaaatccctgga = 5\n+ 39094: aactcaggttttactgagttacttaatcgcgtagaagcct = 5\n+ 42359: aagaacggcatctttagtttgcacgataaccatatcttca = 5\n+ 44118: aagaggcgatgatgcagcacaggagggacgtgttaaagct = 5\n+ 45106: aagatgttttcgcttacgcaacgggacaacgcgatgctga = 6\n+ 46997: aagccgctgctctgccggcattccccttatatccgcctct = 7\n+ 47380: aagcgaaaacatcttctgcaattccacgaaggcgctctcc = 6\n+ 50897: aaggcttctacgcgattaagtaactcagtaaaacctgagt = 5\n+ 51296: aaggggaatgccggcagagcagcggcttgctgaacgtcag = 6\n+ 52678: aagtaccgttgcaccttcccggttacgctccctccccaga = 5\n+ 53910: aagtgctgcacctgctgactcaactgcatgacccgctcgt = 5\n+ 57240: aatacgggcatgatgttactggggatagtctttctcctgt = 5\n+ 65459: aatgccggcagagcagcggcttgctgaacgtcagcgaaaa = 6\n+ 66807: aatggctgctgccagtggcgctttgtcgtgtctttccggt = 5\n+ 66876: aatgggagagcgccttcgtggaattgcagaagatgttttc = 6\n+ 68705: aattagccaccatgccgcgcatctcacacccgcgcatgca = 5\n+ 70117: aattgcagaagatgttttcgcttacgcaacgggacaacgc = 6\n+ 73840: acaacgcgatgctgaacgagcgggtcatgcagttgagtca = 7\n+ 73929: acaacggtttcgtttttcgctgacgttcagcaagccgctg = 5\n+ 79713: acaggagggacgtgttaaagctggcagggaatacgggcat = 7\n+ 81836: acatcatgcccgtattccctgccagctttaacacgtccct = 6\n+ 84684: accaatggctgctgccagtggcgctttgtcgtgtctttcc = 5\n+ 86754: accagctttccagggatttcaacaacggtttcgtttttcg = 5\n+ 87548: accatcaccgcccccggtatcccgctcatgctggcgaacc = 6\n+ 88541: acccaccacggatatccgtaaggacagatgcagcaagtgc = 5\n+ 89933: acccgctcgttcagcatcgcgttgtcccgttgcgtaagcg = 7\n+ 92340: accgcccccggtatcccgctcatgctggcgaacccagaca = 6\n+ 95583: accgttgcaccttcccggttacgctccctccccagaaggt = 5\n+ 95641: accgttgttgaaatccctggaaagctggttgcgtgaaaag = 5\n+ 97042: acctgctgactcaactgcatgacccgctcgttcagcatcg = 7\n+ 98459: acgaaaccgttgttgaaatccctggaaagctggttgcgtg = 5\n+ 101251: acgcaacgggacaacgcgatgctgaacgagcgggtcatgc = 7\n+ 102467: acgccatagaggcggatataaggggaatgccggcagagca = 7\n+ 103655: acgcgatgctgaacgagcgggtcatgcagttgagtcagca = 7\n+ 106812: acggcatctttagtttgcacgataaccatatcttcaattc = 5\n+ 107733: acgggacaacgcgatgctgaacgagcgggtcatgcagttg = 7\n+ 107912: acgggcatgatgttactggggatagtctttctcctgttta = 5\n+ 109184: acggttcaccatgaaccataatatgatttctaagaatacc = 5\n+ 109323: acggtttcgtttttcgctgacgttcagcaagccgctgctc = 5\n+ 109991: acgtcagcgaaaaacgaaaccgttgttgaaatccctggaa = 5\n+ 111273: acgttcagcaagccgctgc'..b'agtcagcaggtgcagcacttga = 5\n+ 397948: gatataaggggaatgccggcagagcagcggcttgctgaac = 7\n+ 402619: gatgttttcgcttacgcaacgggacaacgcgatgctgaac = 6\n+ 402865: gattaccttctggggagggagcgtaaccgggaaggtgcaa = 5\n+ 404021: gatttcaacaacggtttcgtttttcgctgacgttcagcaa = 5\n+ 407354: gcacaggagggacgtgttaaagctggcagggaatacgggc = 7\n+ 409109: gcagagcagcggcttgctgaacgtcagcgaaaaacgaaac = 5\n+ 412106: gcatctttagtttgcacgataaccatatcttcaattccaa = 5\n+ 412209: gcatgatgttactggggatagtctttctcctgtttaccgc = 5\n+ 413124: gccaaaattagccaccatgccgcgcatctcacacccgcgc = 5\n+ 413965: gccactcgcggtaaacaggagaaagactatccccagtaac = 5\n+ 415288: gccatagaggcggatataaggggaatgccggcagagcagc = 7\n+ 416467: gcccccggtatcccgctcatgctggcgaacccagacacgc = 6\n+ 421338: gccttcgtggaattgcagaagatgttttcgcttacgcaac = 6\n+ 421606: gcgaaaacatcttctgcaattccacgaaggcgctctccca = 6\n+ 425114: gcgccttcgtggaattgcagaagatgttttcgcttacgca = 6\n+ 428102: gcgggtcatgcagttgagtcagcaggtgcagcacttgagc = 5\n+ 432431: gctcgttcagcatcgcgttgtcccgttgcgtaagcgaaaa = 7\n+ 432544: gctctgccggcattccccttatatccgcctctatggcgta = 7\n+ 432796: gctgaacgtcagcgaaaaacgaaaccgttgttgaaatccc = 5\n+ 435138: gctgttacttcccgctctaaagtagaagtaacatgaatca = 5\n+ 435931: gcttctacgcgattaagtaactcagtaaaacctgagttaa = 5\n+ 436168: gcttgctgaacgtcagcgaaaaacgaaaccgttgttgaaa = 5\n+ 436476: gctttccagggatttcaacaacggtttcgtttttcgctga = 5\n+ 438332: ggaatgccggcagagcagcggcttgctgaacgtcagcgaa = 6\n+ 438435: ggaattgcagaagatgttttcgcttacgcaacgggacaac = 6\n+ 439017: ggacgtgttaaagctggcagggaatacgggcatgatgtta = 5\n+ 439212: ggagaaagactatccccagtaacatcatgcccgtattccc = 5\n+ 440307: ggatataaggggaatgccggcagagcagcggcttgctgaa = 7\n+ 441337: ggatttcaacaacggtttcgtttttcgctgacgttcagca = 5\n+ 442536: ggcagagcagcggcttgctgaacgtcagcgaaaaacgaaa = 6\n+ 443224: ggcatctttagtttgcacgataaccatatcttcaattcca = 5\n+ 444964: ggcgatgatgcagcacaggagggacgtgttaaagctggca = 5\n+ 447754: ggcttctacgcgattaagtaactcagtaaaacctgagtta = 5\n+ 447808: ggcttgctgaacgtcagcgaaaaacgaaaccgttgttgaa = 5\n+ 448268: gggaatgccggcagagcagcggcttgctgaacgtcagcga = 6\n+ 449217: gggcatgatgttactggggatagtctttctcctgtttacc = 5\n+ 451194: gggtcatgcagttgagtcagcaggtgcagcacttgagcga = 5\n+ 455477: ggttcaccatgaaccataatatgatttctaagaataccaa = 5\n+ 459621: gtattccctgccagctttaacacgtccctcctgtgctgca = 6\n+ 459664: gtattcttagaaatcatattatggttcatggtgaaccgta = 5\n+ 464117: gtgagagcgctgttacttcccgctctaaagtagaagtaac = 5\n+ 465705: gtggaattgcagaagatgttttcgcttacgcaacgggaca = 6\n+ 470168: gttgtacgccatagaggcggatataaggggaatgccggca = 5\n+ 471622: gtttttcgctgacgttcagcaagccgctgctctgccggca = 6\n+ 474188: taaggggaatgccggcagagcagcggcttgctgaacgtca = 6\n+ 477666: tactttagagcgggaagtaacagcgctctcacttacaaaa = 5\n+ 481177: tattcttagaaatcatattatggttcatggtgaaccgtaa = 5\n+ 482917: tcaccgcccccggtatcccgctcatgctggcgaacccaga = 6\n+ 484763: tcatcttttcacgcaaccagctttccagggatttcaacaa = 5\n+ 486368: tcccgttgcgtaagcgaaaacatcttctgcaattccacga = 6\n+ 488979: tcgcgttgtcccgttgcgtaagcgaaaacatcttctgcaa = 6\n+ 490132: tcgttcagcatcgcgttgtcccgttgcgtaagcgaaaaca = 7\n+ 490368: tctactttagagcgggaagtaacagcgctctcacttacaa = 5\n+ 491019: tctgccggcattccccttatatccgcctctatggcgtaca = 7\n+ 491604: tcttcatcttttcacgcaaccagctttccagggatttcaa = 5\n+ 492432: tgaacgagcgggtcatgcagttgagtcagcaggtgcagca = 5\n+ 492674: tgaagtaccgttgcaccttcccggttacgctccctcccca = 5\n+ 493518: tgagagcgctgttacttcccgctctaaagtagaagtaaca = 5\n+ 494230: tgatgcagcacaggagggacgtgttaaagctggcagggaa = 5\n+ 494813: tgcacctgctgactcaactgcatgacccgctcgttcagca = 7\n+ 495175: tgcatttacggttcaccatgaaccataatatgatttctaa = 5\n+ 497093: tggaattgcagaagatgttttcgcttacgcaacgggacaa = 6\n+ 498688: tgtaagtgagagcgctgttacttcccgctctaaagtagaa = 5\n+ 499514: tgttgaaatccctggaaagctggttgcgtgaaaagatgaa = 5\n' |
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| diff -r 000000000000 -r ff827f983bbb test-data/test03.tabex.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/test03.tabex.txt Fri May 03 20:23:04 2024 +0000 |
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| b'@@ -0,0 +1,311 @@\n+Opening 40-mer table with 310 entries occuring 5-or-more times\n+ 0: aaaaacgaaaccgttgttgaaatccctggaaagctggttg = 5\n+ 1: aaaacatcttctgcaattccacgaaggcgctctcccattc = 6\n+ 2: aaaacgaaaccgttgttgaaatccctggaaagctggttgc = 5\n+ 3: aaaattagccaccatgccgcgcatctcacacccgcgcatg = 5\n+ 4: aaacaggagaaagactatccccagtaacatcatgcccgta = 5\n+ 5: aaacatcttctgcaattccacgaaggcgctctcccattcg = 6\n+ 6: aaaccgttgttgaaatccctggaaagctggttgcgtgaaa = 5\n+ 7: aaacgaaaccgttgttgaaatccctggaaagctggttgcg = 5\n+ 8: aaagactatccccagtaacatcatgcccgtattccctgcc = 5\n+ 9: aaattagccaccatgccgcgcatctcacacccgcgcatgc = 5\n+ 10: aacaacggtttcgtttttcgctgacgttcagcaagccgct = 5\n+ 11: aacaggagaaagactatccccagtaacatcatgcccgtat = 5\n+ 12: aacatcatgcccgtattccctgccagctttaacacgtccc = 6\n+ 13: aaccagctttccagggatttcaacaacggtttcgtttttc = 5\n+ 14: aacccaccacggatatccgtaaggacagatgcagcaagtg = 5\n+ 15: aaccggaaagacacgacaaagcgccactggcagcagccat = 5\n+ 16: aaccgttgttgaaatccctggaaagctggttgcgtgaaaa = 5\n+ 17: aacgaaaccgttgttgaaatccctggaaagctggttgcgt = 5\n+ 18: aacgagcgggtcatgcagttgagtcagcaggtgcagcact = 5\n+ 19: aacgcgatgctgaacgagcgggtcatgcagttgagtcagc = 7\n+ 20: aacggcatctttagtttgcacgataaccatatcttcaatt = 5\n+ 21: aacgggacaacgcgatgctgaacgagcgggtcatgcagtt = 7\n+ 22: aacggtttcgtttttcgctgacgttcagcaagccgctgct = 5\n+ 23: aacgtcagcgaaaaacgaaaccgttgttgaaatccctgga = 5\n+ 24: aactcaggttttactgagttacttaatcgcgtagaagcct = 5\n+ 25: aagaacggcatctttagtttgcacgataaccatatcttca = 5\n+ 26: aagaggcgatgatgcagcacaggagggacgtgttaaagct = 5\n+ 27: aagatgttttcgcttacgcaacgggacaacgcgatgctga = 6\n+ 28: aagccgctgctctgccggcattccccttatatccgcctct = 7\n+ 29: aagcgaaaacatcttctgcaattccacgaaggcgctctcc = 6\n+ 30: aaggcttctacgcgattaagtaactcagtaaaacctgagt = 5\n+ 31: aaggggaatgccggcagagcagcggcttgctgaacgtcag = 6\n+ 32: aagtaccgttgcaccttcccggttacgctccctccccaga = 5\n+ 33: aagtgctgcacctgctgactcaactgcatgacccgctcgt = 5\n+ 34: aatacgggcatgatgttactggggatagtctttctcctgt = 5\n+ 35: aatgccggcagagcagcggcttgctgaacgtcagcgaaaa = 6\n+ 36: aatggctgctgccagtggcgctttgtcgtgtctttccggt = 5\n+ 37: aatgggagagcgccttcgtggaattgcagaagatgttttc = 6\n+ 38: aattagccaccatgccgcgcatctcacacccgcgcatgca = 5\n+ 39: aattgcagaagatgttttcgcttacgcaacgggacaacgc = 6\n+ 40: acaacgcgatgctgaacgagcgggtcatgcagttgagtca = 7\n+ 41: acaacggtttcgtttttcgctgacgttcagcaagccgctg = 5\n+ 42: acaggagggacgtgttaaagctggcagggaatacgggcat = 7\n+ 43: acatcatgcccgtattccctgccagctttaacacgtccct = 6\n+ 44: accaatggctgctgccagtggcgctttgtcgtgtctttcc = 5\n+ 45: accagctttccagggatttcaacaacggtttcgtttttcg = 5\n+ 46: accatcaccgcccccggtatcccgctcatgctggcgaacc = 6\n+ 47: acccaccacggatatccgtaaggacagatgcagcaagtgc = 5\n+ 48: acccgctcgttcagcatcgcgttgtcccgttgcgtaagcg = 7\n+ 49: accgcccccggtatcccgctcatgctggcgaacccagaca = 6\n+ 50: accgttgcaccttcccggttacgctccctccccagaaggt = 5\n+ 51: accgttgttgaaatccctggaaagctggttgcgtgaaaag = 5\n+ 52: acctgctgactcaactgcatgacccgctcgttcagcatcg = 7\n+ 53: acgaaaccgttgttgaaatccctggaaagctggttgcgtg = 5\n+ 54: acgcaacgggacaacgcgatgctgaacgagcgggtcatgc = 7\n+ 55: acgccatagaggcggatataaggggaatgccggcagagca = 7\n+ 56: acgcgatgctgaacgagcgggtcatgcagttgagtcagca = 7\n+ 57: acggcatctttagtttgcacgataaccatatcttcaattc = 5\n+ 58: acgggacaacgcgatgctgaacgagcgggtcatgcagttg = 7\n+ 59: acgggcatgatgttactggggatagtctttctcctgttta = 5\n+ 60: acggttcaccatgaaccataatatgatttctaagaatacc = 5\n+ 61: acggtttcgtttttcgctgacgttcagcaagccgctgctc = 5\n+ 62: acgtcagcgaaaaacgaaaccgttgttgaaatccctggaa = 5\n+ 63'..b'agtcagcaggtgcagcacttga = 5\n+ 246: gatataaggggaatgccggcagagcagcggcttgctgaac = 7\n+ 247: gatgttttcgcttacgcaacgggacaacgcgatgctgaac = 6\n+ 248: gattaccttctggggagggagcgtaaccgggaaggtgcaa = 5\n+ 249: gatttcaacaacggtttcgtttttcgctgacgttcagcaa = 5\n+ 250: gcacaggagggacgtgttaaagctggcagggaatacgggc = 7\n+ 251: gcagagcagcggcttgctgaacgtcagcgaaaaacgaaac = 5\n+ 252: gcatctttagtttgcacgataaccatatcttcaattccaa = 5\n+ 253: gcatgatgttactggggatagtctttctcctgtttaccgc = 5\n+ 254: gccaaaattagccaccatgccgcgcatctcacacccgcgc = 5\n+ 255: gccactcgcggtaaacaggagaaagactatccccagtaac = 5\n+ 256: gccatagaggcggatataaggggaatgccggcagagcagc = 7\n+ 257: gcccccggtatcccgctcatgctggcgaacccagacacgc = 6\n+ 258: gccttcgtggaattgcagaagatgttttcgcttacgcaac = 6\n+ 259: gcgaaaacatcttctgcaattccacgaaggcgctctccca = 6\n+ 260: gcgccttcgtggaattgcagaagatgttttcgcttacgca = 6\n+ 261: gcgggtcatgcagttgagtcagcaggtgcagcacttgagc = 5\n+ 262: gctcgttcagcatcgcgttgtcccgttgcgtaagcgaaaa = 7\n+ 263: gctctgccggcattccccttatatccgcctctatggcgta = 7\n+ 264: gctgaacgtcagcgaaaaacgaaaccgttgttgaaatccc = 5\n+ 265: gctgttacttcccgctctaaagtagaagtaacatgaatca = 5\n+ 266: gcttctacgcgattaagtaactcagtaaaacctgagttaa = 5\n+ 267: gcttgctgaacgtcagcgaaaaacgaaaccgttgttgaaa = 5\n+ 268: gctttccagggatttcaacaacggtttcgtttttcgctga = 5\n+ 269: ggaatgccggcagagcagcggcttgctgaacgtcagcgaa = 6\n+ 270: ggaattgcagaagatgttttcgcttacgcaacgggacaac = 6\n+ 271: ggacgtgttaaagctggcagggaatacgggcatgatgtta = 5\n+ 272: ggagaaagactatccccagtaacatcatgcccgtattccc = 5\n+ 273: ggatataaggggaatgccggcagagcagcggcttgctgaa = 7\n+ 274: ggatttcaacaacggtttcgtttttcgctgacgttcagca = 5\n+ 275: ggcagagcagcggcttgctgaacgtcagcgaaaaacgaaa = 6\n+ 276: ggcatctttagtttgcacgataaccatatcttcaattcca = 5\n+ 277: ggcgatgatgcagcacaggagggacgtgttaaagctggca = 5\n+ 278: ggcttctacgcgattaagtaactcagtaaaacctgagtta = 5\n+ 279: ggcttgctgaacgtcagcgaaaaacgaaaccgttgttgaa = 5\n+ 280: gggaatgccggcagagcagcggcttgctgaacgtcagcga = 6\n+ 281: gggcatgatgttactggggatagtctttctcctgtttacc = 5\n+ 282: gggtcatgcagttgagtcagcaggtgcagcacttgagcga = 5\n+ 283: ggttcaccatgaaccataatatgatttctaagaataccaa = 5\n+ 284: gtattccctgccagctttaacacgtccctcctgtgctgca = 6\n+ 285: gtattcttagaaatcatattatggttcatggtgaaccgta = 5\n+ 286: gtgagagcgctgttacttcccgctctaaagtagaagtaac = 5\n+ 287: gtggaattgcagaagatgttttcgcttacgcaacgggaca = 6\n+ 288: gttgtacgccatagaggcggatataaggggaatgccggca = 5\n+ 289: gtttttcgctgacgttcagcaagccgctgctctgccggca = 6\n+ 290: taaggggaatgccggcagagcagcggcttgctgaacgtca = 6\n+ 291: tactttagagcgggaagtaacagcgctctcacttacaaaa = 5\n+ 292: tattcttagaaatcatattatggttcatggtgaaccgtaa = 5\n+ 293: tcaccgcccccggtatcccgctcatgctggcgaacccaga = 6\n+ 294: tcatcttttcacgcaaccagctttccagggatttcaacaa = 5\n+ 295: tcccgttgcgtaagcgaaaacatcttctgcaattccacga = 6\n+ 296: tcgcgttgtcccgttgcgtaagcgaaaacatcttctgcaa = 6\n+ 297: tcgttcagcatcgcgttgtcccgttgcgtaagcgaaaaca = 7\n+ 298: tctactttagagcgggaagtaacagcgctctcacttacaa = 5\n+ 299: tctgccggcattccccttatatccgcctctatggcgtaca = 7\n+ 300: tcttcatcttttcacgcaaccagctttccagggatttcaa = 5\n+ 301: tgaacgagcgggtcatgcagttgagtcagcaggtgcagca = 5\n+ 302: tgaagtaccgttgcaccttcccggttacgctccctcccca = 5\n+ 303: tgagagcgctgttacttcccgctctaaagtagaagtaaca = 5\n+ 304: tgatgcagcacaggagggacgtgttaaagctggcagggaa = 5\n+ 305: tgcacctgctgactcaactgcatgacccgctcgttcagca = 7\n+ 306: tgcatttacggttcaccatgaaccataatatgatttctaa = 5\n+ 307: tggaattgcagaagatgttttcgcttacgcaacgggacaa = 6\n+ 308: tgtaagtgagagcgctgttacttcccgctctaaagtagaa = 5\n+ 309: tgttgaaatccctggaaagctggttgcgtgaaaagatgaa = 5\n' |