Repository 'samtools_mpileup'
hg clone https://toolshed.g2.bx.psu.edu/repos/devteam/samtools_mpileup

Changeset 8:583abf29fc8e (2017-05-09)
Previous changeset 7:bfc4517aa037 (2015-11-11) Next changeset 9:fa7ad9b89f4a (2018-09-03)
Commit message:
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools/samtools_mpileup commit 411130b45dc30f7f24f41cdeec5e148c5d8faf40
modified:
macros.xml
samtools_mpileup.xml
test-data/samtools_mpileup_out_1.pileup
test-data/samtools_mpileup_out_2.vcf
added:
test-data/samtools_mpileup_out_3.pileup
removed:
test-data/samtools/mpileup/samtools_mpileup_out_1.log
test-data/samtools/mpileup/samtools_mpileup_out_1.pileup
test-data/samtools/mpileup/samtools_mpileup_out_2.bcf
test-data/samtools_mpileup_out_1.log
test-data/samtools_mpileup_out_2.log
test-data/samtools_mpileup_out_3.log
test-data/samtools_mpileup_out_4.log
tool_dependencies.xml
b
diff -r bfc4517aa037 -r 583abf29fc8e macros.xml
--- a/macros.xml Wed Nov 11 12:53:32 2015 -0500
+++ b/macros.xml Tue May 09 11:17:20 2017 -0400
[
@@ -1,16 +1,17 @@
 <macros>
     <xml name="requirements">
         <requirements>
-            <requirement type="package" version="1.2">samtools</requirement>
+            <requirement type="package" version="1.3.1">samtools</requirement>
             <yield/>
         </requirements>
     </xml>
+    <token name="@TOOL_VERSION@">1.3.1</token>
     <xml name="citations">
         <citations>
             <citation type="bibtex">
                 @misc{SAM_def,
                 title={Definition of SAM/BAM format},
-                url = {https://samtools.github.io/hts-specs/SAMv1.pdf},}
+                url = {https://samtools.github.io/hts-specs/},}
             </citation>
             <citation type="doi">10.1093/bioinformatics/btp352</citation>
             <citation type="doi">10.1093/bioinformatics/btr076</citation>
@@ -41,7 +42,7 @@
         </citations>
     </xml>
     <xml name="version_command">
-        <version_command>samtools --version | head -n 1 | awk '{ print $2 }'</version_command>
+        <version_command><![CDATA[samtools 2>&1 | grep Version]]></version_command>
     </xml>
     <xml name="stdio">
         <stdio>
@@ -64,7 +65,5 @@
 5. Click **Save**
 
 The medatada will be re-detected and you will be able to see the list of reference sequences in the &quot;**Select references (chromosomes and contigs) you would like to restrict bam to**&quot; drop-down.
-
     </token>
-
 </macros>
b
diff -r bfc4517aa037 -r 583abf29fc8e samtools_mpileup.xml
--- a/samtools_mpileup.xml Wed Nov 11 12:53:32 2015 -0500
+++ b/samtools_mpileup.xml Tue May 09 11:17:20 2017 -0400
[
b'@@ -1,25 +1,30 @@\n-<tool id="samtools_mpileup" name="MPileup" version="2.1.1">\n-    <description>call variants</description>\n+<tool id="samtools_mpileup" name="MPileup" version="2.1.3">\n+    <description>multi-way pileup of variants</description>\n     <macros>\n         <import>macros.xml</import>\n     </macros>\n     <expand macro="requirements" />\n     <expand macro="stdio" />\n     <expand macro="version_command" />\n-    <command>\n-    <![CDATA[\n+    <command><![CDATA[\n+    #for $bam_count, $input_bam in enumerate( $reference_source.input_bam ):\n+        ln -s \'${input_bam}\' \'localbam_${bam_count}.bam\' &&\n+        ln -s \'${input_bam.metadata.bam_index}\' \'localbam_${bam_count}.bam.bai\' &&\n+    #end for\n+\n     #if $reference_source.reference_source_selector == "history":\n-       ln -s "${reference_source.ref_file}" && samtools faidx `basename "${reference_source.ref_file}"` && samtools mpileup\n-    #else:\n-        samtools mpileup\n+        ln -s \'${reference_source.ref_file}\' &&\n+        samtools faidx `basename \'${reference_source.ref_file}\'` &&\n     #end if\n+\n+    samtools mpileup\n     #if $reference_source.reference_source_selector != "history":\n-        -f "${reference_source.ref_file.fields.path}"\n+        -f \'${reference_source.ref_file.fields.path}\'\n     #else:\n-        -f "${reference_source.ref_file}"\n+        -f \'${reference_source.ref_file}\'\n     #end if\n-    #for $bam in $reference_source.input_bam:\n-        "${bam}"\n+    #for $bam_count, $input_bam in enumerate( $reference_source.input_bam ):\n+        localbam_${bam_count}.bam\n     #end for\n     #if str( $advanced_options.advanced_options_selector ) == "advanced":\n         #if str( $advanced_options.filter_by_flags.filter_flags ) == "filter":\n@@ -31,95 +36,110 @@\n             #end if\n         #end if\n         #if str( $advanced_options.limit_by_region.limit_by_regions ) == "paste":\n-            -l "$pasted_regions"\n+            -l \'$pasted_regions\'\n         #elif str( $advanced_options.limit_by_region.limit_by_regions ) == "history"\n-            -l "$advanced_options.limit_by_region.bed_regions"\n+            -l \'$advanced_options.limit_by_region.bed_regions\'\n         #end if\n         #if str( $advanced_options.exclude_read_group.exclude_read_groups ) == "paste":\n-            -G "$excluded_read_groups"\n+            -G \'$excluded_read_groups\'\n         #elif str( $advanced_options.exclude_read_group.exclude_read_groups ) == "history"\n-            -G "$advanced_options.exclude_read_group.read_groups"\n+            -G \'$advanced_options.exclude_read_group.read_groups\'\n         #end if\n         ${advanced_options.skip_anomalous_read_pairs}\n         ${advanced_options.disable_probabilistic_realignment}\n-        -C "${advanced_options.coefficient_for_downgrading}"\n-        -d "${advanced_options.max_reads_per_bam}"\n+        -C ${advanced_options.coefficient_for_downgrading}\n+        -d ${advanced_options.max_reads_per_bam}\n         ${advanced_options.extended_BAQ_computation}\n-        -q "${advanced_options.minimum_mapping_quality}"\n-        -Q "${advanced_options.minimum_base_quality}"\n+        -q ${advanced_options.minimum_mapping_quality}\n+        -Q ${advanced_options.minimum_base_quality}\n         #if str( $advanced_options.region_string ):\n-            -r "${advanced_options.region_string}"\n+            -r \'${advanced_options.region_string}\'\n         #end if\n-\n     #end if\n     #if str( $genotype_likelihood_computation_type.genotype_likelihood_computation_type_selector ) == \'perform_genotype_likelihood_computation\':\n-        ##\n-\n         ${genotype_likelihood_computation_type.output_format}\n         ${genotype_likelihood_computation_type.compressed}\n \n         #if str( $genotype_likelihood_computation_type.output_tags ) != "None":\n-            --output-tags "${genotype_likelihood_computation_type.output_tags}"\n+            --output-tags \'${genotype_likelihood_computation_type.output_tags}\'\n         #end if\n \n         #if str( $genotype_likelihood_computation_type.per'..b's. Alignment records are grouped by sample identifiers in @RG header lines. If sample identifiers are absent, each input file is regarded as one sample.\n-\n-------\n-\n-**Input options**::\n-\n-  -6, --illumina1.3+      quality is in the Illumina-1.3+ encoding\n-  -A, --count-orphans     do not discard anomalous read pairs\n-  -b, --bam-list FILE     list of input BAM filenames, one per line\n-  -B, --no-BAQ            disable BAQ (per-Base Alignment Quality)\n-  -C, --adjust-MQ INT     adjust mapping quality; recommended:50, disable:0 [0]\n-  -d, --max-depth INT     max per-BAM depth; avoids excessive memory usage [250]\n-  -E, --redo-BAQ          recalculate BAQ on the fly, ignore existing BQs\n-  -f, --fasta-ref FILE    faidx indexed reference sequence file\n-  -G, --exclude-RG FILE   exclude read groups listed in FILE\n-  -l, --positions FILE    skip unlisted positions (chr pos) or regions (BED)\n-  -q, --min-MQ INT        skip alignments with mapQ smaller than INT [0]\n-  -Q, --min-BQ INT        skip bases with baseQ/BAQ smaller than INT [13]\n-  -r, --region REG        region in which pileup is generated\n-  -R, --ignore-RG         ignore RG tags (one BAM = one sample)\n-  --rf, --incl-flags STR|INT  required flags: skip reads with mask bits unset []\n-  --ff, --excl-flags STR|INT  filter flags: skip reads with mask bits set\n-                                            [UNMAP,SECONDARY,QCFAIL,DUP]\n-  -x, --ignore-overlaps   disable read-pair overlap detection\n-\n-**Output options**::\n-\n-  -o, --output FILE       write output to FILE [standard output]\n-  -g, --BCF               generate genotype likelihoods in BCF format\n-  -v, --VCF               generate genotype likelihoods in VCF format\n-\n-**Output options for mpileup format** (without -g/-v)::\n-\n-  -O, --output-BP         output base positions on reads\n-  -s, --output-MQ         output mapping quality\n-\n-**Output options for genotype likelihoods** (when -g/-v is used)::\n-\n-  -t, --output-tags LIST  optional tags to output: DP,DPR,DV,DP4,INFO/DPR,SP []\n-  -u, --uncompressed      generate uncompressed VCF/BCF output\n-\n-**SNP/INDEL genotype likelihoods options** (effective with -g/-v)::\n-\n-  -e, --ext-prob INT      Phred-scaled gap extension seq error probability [20]\n-  -F, --gap-frac FLOAT    minimum fraction of gapped reads [0.002]\n-  -h, --tandem-qual INT   coefficient for homopolymer errors [100]\n-  -I, --skip-indels       do not perform indel calling\n-  -L, --max-idepth INT    maximum per-sample depth for INDEL calling [250]\n-  -m, --min-ireads INT    minimum number gapped reads for indel candidates [1]\n-  -o, --open-prob INT     Phred-scaled gap open seq error probability [40]\n-  -p, --per-sample-mF     apply -m and -F per-sample for increased sensitivity\n-  -P, --platforms STR     comma separated list of platforms for indels [all]\n+Report variants for one or multiple BAM files. Alignment records are grouped by sample identifiers in @RG header lines.\n+If sample identifiers are absent, each input file is regarded as one sample.\n \n **Notes**: Assuming diploid individuals.\n-]]>\n-    </help>\n-    <configfiles>\n-        <configfile name="excluded_read_groups">\n-<![CDATA[\n-#set pasted_data = \'\'\n-#if str( $advanced_options.advanced_options_selector ) == "advanced":\n-    #if str( $advanced_options.exclude_read_group.exclude_read_groups ) == "paste":\n-        #set pasted_data = \'\\t\'.join( str( $advanced_options.exclude_read_group[\'read_groups\'] ).split() )\n-    #end if\n-#end if\n-${pasted_data}\n-]]>\n-        </configfile>\n-        <configfile name="pasted_regions">\n-<![CDATA[\n-#set pasted_data = \'\'\n-#if str( $advanced_options.advanced_options_selector ) == "advanced":\n-    #if str( $advanced_options.limit_by_region.limit_by_regions ) == "paste":\n-        #set pasted_data = \'\\t\'.join( str( $advanced_options.limit_by_region[\'region_paste\'] ).split() )\n-    #end if\n-#end if\n-${pasted_data}\n-]]>\n-        </configfile>\n-    </configfiles>\n+    ]]></help>\n     <expand macro="citations" />\n </tool>\n'
b
diff -r bfc4517aa037 -r 583abf29fc8e test-data/samtools/mpileup/samtools_mpileup_out_1.log
--- a/test-data/samtools/mpileup/samtools_mpileup_out_1.log Wed Nov 11 12:53:32 2015 -0500
+++ /dev/null Thu Jan 01 00:00:00 1970 +0000
[
@@ -1,2 +0,0 @@
-[mpileup] 1 samples in 1 input files
-<mpileup> Set max per-file depth to 8000
b
diff -r bfc4517aa037 -r 583abf29fc8e test-data/samtools/mpileup/samtools_mpileup_out_1.pileup
--- a/test-data/samtools/mpileup/samtools_mpileup_out_1.pileup Wed Nov 11 12:53:32 2015 -0500
+++ /dev/null Thu Jan 01 00:00:00 1970 +0000
b
@@ -1,43 +0,0 @@
-phiX174 1411 A 1 ^P. $
-phiX174 1412 G 3 .^D.^F. "$$
-phiX174 1413 C 5 ...^D.^F. """$$
-phiX174 1414 G 6 .....^F. #####$
-phiX174 1415 C 7 ......^F. %%%%%%&
-phiX174 1416 C 8 .......^F. $$$$$$$$
-phiX174 1417 G 9 ........^F. "#######$
-phiX174 1418 T 10 .........^F. """""""""$
-phiX174 1419 G 10 .......... """""'&'%$
-phiX174 1420 G 10 .......... """"""""""
-phiX174 1421 A 10 .......... """"""""""
-phiX174 1422 T 10 .......... """"""""""
-phiX174 1423 G 10 .......... """""""""#
-phiX174 1424 C 10 ..A.AAAAAA %"""""""""
-phiX174 1425 C 10 .......... $$$"""""""
-phiX174 1426 T 10 .......... #####"""""
-phiX174 1427 G 10 .......... ######""""
-phiX174 1428 A 10 .......... """"""""""
-phiX174 1429 C 10 .......... ((((((&(""
-phiX174 1430 C 10 .......... $$$$$$$$$"
-phiX174 1431 G 10 .......... ##########
-phiX174 1432 T 10 .......... """"""""""
-phiX174 1433 A 10 .......... ##########
-phiX174 1434 C 10 .......... ((((((&(%$
-phiX174 1435 C 10 .......... $$$$$$$$$$
-phiX174 1436 G 10 .......... ##########
-phiX174 1437 A 10 .......... """""""""!
-phiX174 1438 G 10 .......... """""####!
-phiX174 1439 G 10 .......... """""""""!
-phiX174 1440 C 10 .......... """""""""!
-phiX174 1441 T 10 .......... """"""""#!
-phiX174 1442 A 10 .......... $$$%%%&&%!
-phiX174 1443 A 10 .-1C.-1C..-1C...... """""""""!
-phiX174 1444 C 10 **.*...... &%"!"""""!
-phiX174 1445 C 10 .......... &%&!%%%&%!
-phiX174 1446 C 10 .......... """!"""""!
-phiX174 1447 T 10 .$..$....... #"#!"""""!
-phiX174 1448 A 8 .$..$..... #!#%%$$!
-phiX174 1449 A 6 .$.$.... !""""!
-phiX174 1450 T 4 .$... """!
-phiX174 1451 G 3 .$.. #"!
-phiX174 1452 A 2 .$. "!
-phiX174 1453 G 1 .$ !
b
diff -r bfc4517aa037 -r 583abf29fc8e test-data/samtools/mpileup/samtools_mpileup_out_2.bcf
b
Binary file test-data/samtools/mpileup/samtools_mpileup_out_2.bcf has changed
b
diff -r bfc4517aa037 -r 583abf29fc8e test-data/samtools_mpileup_out_1.log
--- a/test-data/samtools_mpileup_out_1.log Wed Nov 11 12:53:32 2015 -0500
+++ /dev/null Thu Jan 01 00:00:00 1970 +0000
[
@@ -1,3 +0,0 @@
-[fai_load] build FASTA index.
-[mpileup] 1 samples in 1 input files
-<mpileup> Set max per-file depth to 8000
b
diff -r bfc4517aa037 -r 583abf29fc8e test-data/samtools_mpileup_out_1.pileup
--- a/test-data/samtools_mpileup_out_1.pileup Wed Nov 11 12:53:32 2015 -0500
+++ b/test-data/samtools_mpileup_out_1.pileup Tue May 09 11:17:20 2017 -0400
b
@@ -1,43 +1,43 @@
-phiX174 1411 A 0 1
-phiX174 1412 G 0 2,1,1
-phiX174 1413 C 0 3,2,2,1,1
-phiX174 1414 G 0 4,3,3,2,2,1
-phiX174 1415 C 0 5,4,4,3,3,2,1
-phiX174 1416 C 0 6,5,5,4,4,3,2,1
-phiX174 1417 G 0 7,6,6,5,5,4,3,2,1
-phiX174 1418 T 0 8,7,7,6,6,5,4,3,2,1
-phiX174 1419 G 0 9,8,8,7,7,6,5,4,3,2
-phiX174 1420 G 0 10,9,9,8,8,7,6,5,4,3
-phiX174 1421 A 0 11,10,10,9,9,8,7,6,5,4
-phiX174 1422 T 0 12,11,11,10,10,9,8,7,6,5
-phiX174 1423 G 0 13,12,12,11,11,10,9,8,7,6
-phiX174 1424 C 0 14,13,13,12,12,11,10,9,8,7
-phiX174 1425 C 0 15,14,14,13,13,12,11,10,9,8
-phiX174 1426 T 0 16,15,15,14,14,13,12,11,10,9
-phiX174 1427 G 0 17,16,16,15,15,14,13,12,11,10
-phiX174 1428 A 0 18,17,17,16,16,15,14,13,12,11
-phiX174 1429 C 0 19,18,18,17,17,16,15,14,13,12
-phiX174 1430 C 0 20,19,19,18,18,17,16,15,14,13
-phiX174 1431 G 0 21,20,20,19,19,18,17,16,15,14
-phiX174 1432 T 0 22,21,21,20,20,19,18,17,16,15
-phiX174 1433 A 0 23,22,22,21,21,20,19,18,17,16
-phiX174 1434 C 0 24,23,23,22,22,21,20,19,18,17
-phiX174 1435 C 0 25,24,24,23,23,22,21,20,19,18
-phiX174 1436 G 0 26,25,25,24,24,23,22,21,20,19
-phiX174 1437 A 0 27,26,26,25,25,24,23,22,21,20
-phiX174 1438 G 0 28,27,27,26,26,25,24,23,22,21
-phiX174 1439 G 0 29,28,28,27,27,26,25,24,23,22
-phiX174 1440 C 0 30,29,29,28,28,27,26,25,24,23
-phiX174 1441 T 0 31,30,30,29,29,28,27,26,25,24
-phiX174 1442 A 0 32,31,31,30,30,29,28,27,26,25
-phiX174 1443 A 0 33,32,32,31,31,30,29,28,27,26
-phiX174 1444 C 0 34,33,33,32,32,31,30,29,28,27
-phiX174 1445 C 0 34,33,34,32,33,32,31,30,29,28
-phiX174 1446 C 0 35,34,35,33,34,33,32,31,30,29
-phiX174 1447 T 0 36,35,36,34,35,34,33,32,31,30
-phiX174 1448 A 0 36,35,36,35,34,33,32,31
-phiX174 1449 A 0 36,36,35,34,33,32
-phiX174 1450 T 0 36,35,34,33
-phiX174 1451 G 0 36,35,34
-phiX174 1452 A 0 36,35
-phiX174 1453 G 0 36
+phiX174 1411 A 0
+phiX174 1412 G 0
+phiX174 1413 C 0
+phiX174 1414 G 0
+phiX174 1415 C 0
+phiX174 1416 C 0
+phiX174 1417 G 0
+phiX174 1418 T 0
+phiX174 1419 G 0
+phiX174 1420 G 0
+phiX174 1421 A 0
+phiX174 1422 T 0
+phiX174 1423 G 0
+phiX174 1424 C 0
+phiX174 1425 C 0
+phiX174 1426 T 0
+phiX174 1427 G 0
+phiX174 1428 A 0
+phiX174 1429 C 0
+phiX174 1430 C 0
+phiX174 1431 G 0
+phiX174 1432 T 0
+phiX174 1433 A 0
+phiX174 1434 C 0
+phiX174 1435 C 0
+phiX174 1436 G 0
+phiX174 1437 A 0
+phiX174 1438 G 0
+phiX174 1439 G 0
+phiX174 1440 C 0
+phiX174 1441 T 0
+phiX174 1442 A 0
+phiX174 1443 A 0
+phiX174 1444 C 0
+phiX174 1445 C 0
+phiX174 1446 C 0
+phiX174 1447 T 0
+phiX174 1448 A 0
+phiX174 1449 A 0
+phiX174 1450 T 0
+phiX174 1451 G 0
+phiX174 1452 A 0
+phiX174 1453 G 0
b
diff -r bfc4517aa037 -r 583abf29fc8e test-data/samtools_mpileup_out_2.log
--- a/test-data/samtools_mpileup_out_2.log Wed Nov 11 12:53:32 2015 -0500
+++ /dev/null Thu Jan 01 00:00:00 1970 +0000
[
@@ -1,3 +0,0 @@
-[fai_load] build FASTA index.
-[mpileup] 1 samples in 1 input files
-<mpileup> Set max per-file depth to 8000
b
diff -r bfc4517aa037 -r 583abf29fc8e test-data/samtools_mpileup_out_2.vcf
--- a/test-data/samtools_mpileup_out_2.vcf Wed Nov 11 12:53:32 2015 -0500
+++ b/test-data/samtools_mpileup_out_2.vcf Tue May 09 11:17:20 2017 -0400
b
@@ -4,7 +4,7 @@
 ##samtoolsCommand=samtools mpileup --VCF --uncompressed -f /tmp/tmpId8vOP/tmp3bubIE/database/files/000/dataset_735.dat -g -e 20 -h 100 -L 250 -m 1 --open-prob 40 -F 0.002 -e 40 --output /tmp/tmpId8vOP/tmp3bubIE/database/files/000/dataset_736.dat /tmp/tmpId8vOP/tmp3bubIE/database/files/000/dataset_734.dat
 ##reference=file:///tmp/tmpId8vOP/tmp3bubIE/database/files/000/dataset_735.dat
 ##contig=<ID=phiX174,length=5386>
-##ALT=<ID=X,Description="Represents allele(s) other than observed.">
+##ALT=<ID=*,Description="Represents allele(s) other than observed.">
 ##INFO=<ID=INDEL,Number=0,Type=Flag,Description="Indicates that the variant is an INDEL.">
 ##INFO=<ID=IDV,Number=1,Type=Integer,Description="Maximum number of reads supporting an indel">
 ##INFO=<ID=IMF,Number=1,Type=Float,Description="Maximum fraction of reads supporting an indel">
@@ -19,4 +19,4 @@
 ##INFO=<ID=I16,Number=16,Type=Float,Description="Auxiliary tag used for calling, see description of bcf_callret1_t in bam2bcf.h">
 ##INFO=<ID=QS,Number=R,Type=Float,Description="Auxiliary tag used for calling">
 ##FORMAT=<ID=PL,Number=G,Type=Integer,Description="List of Phred-scaled genotype likelihoods">
-#CHROM POS ID REF ALT QUAL FILTER INFO FORMAT /tmp/tmpId8vOP/tmp3bubIE/database/files/000/dataset_734.dat
+#CHROM POS ID REF ALT QUAL FILTER INFO FORMAT localbam_0.bam
b
diff -r bfc4517aa037 -r 583abf29fc8e test-data/samtools_mpileup_out_3.log
--- a/test-data/samtools_mpileup_out_3.log Wed Nov 11 12:53:32 2015 -0500
+++ /dev/null Thu Jan 01 00:00:00 1970 +0000
[
@@ -1,2 +0,0 @@
-[mpileup] 1 samples in 1 input files
-<mpileup> Set max per-file depth to 8000
b
diff -r bfc4517aa037 -r 583abf29fc8e test-data/samtools_mpileup_out_3.pileup
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/samtools_mpileup_out_3.pileup Tue May 09 11:17:20 2017 -0400
b
@@ -0,0 +1,43 @@
+phiX174 1411 A 1 ^P. $ P 1
+phiX174 1412 G 3 .^D.^F. "$$ PDF 2,1,1
+phiX174 1413 C 5 ...^D.^F. """$$ PDFDF 3,2,2,1,1
+phiX174 1414 G 6 .....^F. #####$ PDFDFF 4,3,3,2,2,1
+phiX174 1415 C 7 ......^F. %%%%%%& PDFDFFF 5,4,4,3,3,2,1
+phiX174 1416 C 8 .......^F. $$$$$$$$ PDFDFFFF 6,5,5,4,4,3,2,1
+phiX174 1417 G 9 ........^F. "#######$ PDFDFFFFF 7,6,6,5,5,4,3,2,1
+phiX174 1418 T 10 .........^F. """""""""$ PDFDFFFFFF 8,7,7,6,6,5,4,3,2,1
+phiX174 1419 G 10 .......... """""'&'%$ PDFDFFFFFF 9,8,8,7,7,6,5,4,3,2
+phiX174 1420 G 10 .......... """""""""" PDFDFFFFFF 10,9,9,8,8,7,6,5,4,3
+phiX174 1421 A 10 .......... """""""""" PDFDFFFFFF 11,10,10,9,9,8,7,6,5,4
+phiX174 1422 T 10 .......... """""""""" PDFDFFFFFF 12,11,11,10,10,9,8,7,6,5
+phiX174 1423 G 10 .......... """""""""# PDFDFFFFFF 13,12,12,11,11,10,9,8,7,6
+phiX174 1424 C 10 ..A.AAAAAA %""""""""" PDFDFFFFFF 14,13,13,12,12,11,10,9,8,7
+phiX174 1425 C 10 .......... $$$""""""" PDFDFFFFFF 15,14,14,13,13,12,11,10,9,8
+phiX174 1426 T 10 .......... #####""""" PDFDFFFFFF 16,15,15,14,14,13,12,11,10,9
+phiX174 1427 G 10 .......... ######"""" PDFDFFFFFF 17,16,16,15,15,14,13,12,11,10
+phiX174 1428 A 10 .......... """""""""" PDFDFFFFFF 18,17,17,16,16,15,14,13,12,11
+phiX174 1429 C 10 .......... ((((((&("" PDFDFFFFFF 19,18,18,17,17,16,15,14,13,12
+phiX174 1430 C 10 .......... $$$$$$$$$" PDFDFFFFFF 20,19,19,18,18,17,16,15,14,13
+phiX174 1431 G 10 .......... ########## PDFDFFFFFF 21,20,20,19,19,18,17,16,15,14
+phiX174 1432 T 10 .......... """""""""" PDFDFFFFFF 22,21,21,20,20,19,18,17,16,15
+phiX174 1433 A 10 .......... ########## PDFDFFFFFF 23,22,22,21,21,20,19,18,17,16
+phiX174 1434 C 10 .......... ((((((&(%$ PDFDFFFFFF 24,23,23,22,22,21,20,19,18,17
+phiX174 1435 C 10 .......... $$$$$$$$$$ PDFDFFFFFF 25,24,24,23,23,22,21,20,19,18
+phiX174 1436 G 10 .......... ########## PDFDFFFFFF 26,25,25,24,24,23,22,21,20,19
+phiX174 1437 A 10 .......... """""""""! PDFDFFFFFF 27,26,26,25,25,24,23,22,21,20
+phiX174 1438 G 10 .......... """""####! PDFDFFFFFF 28,27,27,26,26,25,24,23,22,21
+phiX174 1439 G 10 .......... """""""""! PDFDFFFFFF 29,28,28,27,27,26,25,24,23,22
+phiX174 1440 C 10 .......... """""""""! PDFDFFFFFF 30,29,29,28,28,27,26,25,24,23
+phiX174 1441 T 10 .......... """"""""#! PDFDFFFFFF 31,30,30,29,29,28,27,26,25,24
+phiX174 1442 A 10 .......... $$$%%%&&%! PDFDFFFFFF 32,31,31,30,30,29,28,27,26,25
+phiX174 1443 A 10 .-1C.-1C..-1C...... """""""""! PDFDFFFFFF 33,32,32,31,31,30,29,28,27,26
+phiX174 1444 C 10 **.*...... !!"!"""""! PDFDFFFFFF 34,33,33,32,32,31,30,29,28,27
+phiX174 1445 C 10 .......... !!&!%%%&%! PDFDFFFFFF 34,33,34,32,33,32,31,30,29,28
+phiX174 1446 C 10 .......... !!"!"""""! PDFDFFFFFF 35,34,35,33,34,33,32,31,30,29
+phiX174 1447 T 10 .$..$....... !!#!"""""! PDFDFFFFFF 36,35,36,34,35,34,33,32,31,30
+phiX174 1448 A 8 .$..$..... !!#%%$$! DDFFFFFF 36,35,36,35,34,33,32,31
+phiX174 1449 A 6 .$.$.... !""""! DFFFFF 36,36,35,34,33,32
+phiX174 1450 T 4 .$... """! FFFF 36,35,34,33
+phiX174 1451 G 3 .$.. #"! FFF 36,35,34
+phiX174 1452 A 2 .$. "! FF 36,35
+phiX174 1453 G 1 .$ ! F 36
b
diff -r bfc4517aa037 -r 583abf29fc8e test-data/samtools_mpileup_out_4.log
--- a/test-data/samtools_mpileup_out_4.log Wed Nov 11 12:53:32 2015 -0500
+++ /dev/null Thu Jan 01 00:00:00 1970 +0000
[
@@ -1,2 +0,0 @@
-[mpileup] 1 samples in 1 input files
-<mpileup> Set max per-file depth to 8000
b
diff -r bfc4517aa037 -r 583abf29fc8e tool_dependencies.xml
--- a/tool_dependencies.xml Wed Nov 11 12:53:32 2015 -0500
+++ /dev/null Thu Jan 01 00:00:00 1970 +0000
b
@@ -1,6 +0,0 @@
-<?xml version="1.0"?>
-<tool_dependency>
-    <package name="samtools" version="1.2">
-        <repository changeset_revision="f6ae3ba3f3c1" name="package_samtools_1_2" owner="iuc" toolshed="https://toolshed.g2.bx.psu.edu" />
-    </package>
-</tool_dependency>