Repository 'deeptools_compute_matrix'
hg clone https://toolshed.g2.bx.psu.edu/repos/bgruening/deeptools_compute_matrix

Changeset 1:275ed3e83de0 (2016-02-15)
Previous changeset 0:14cb57237e46 (2016-01-25) Next changeset 2:df1ee352b23b (2016-02-18)
Commit message:
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit fef8b344925620444d93d8159c0b2731a5777920
modified:
computeMatrix.xml
deepTools_macros.xml
readme.rst
test-data/bamCompare_result2.bw
test-data/bamCoverage_result1.bw
test-data/bamCoverage_result2.bw
test-data/bamCoverage_result4.bw
test-data/bamPEFragmentSize_result1.txt
test-data/bigwigCompare_result1.bw
test-data/computeMatrix2.bed
test-data/computeMatrix2.bw
test-data/correctGCBias_result1.bam
test-data/plotCoverage_result1.png
test-data/plotCoverage_result1.tabular
test-data/sequence.2bit
test-data/test.bw
tool-data/deepTools_seqs.loc.sample
tool_dependencies.xml
added:
deepTools_macros.xml.orig
static/images/GC_bias_simulated_reads_2L.png
static/images/QC_plotCoverage.png
static/images/bamCompare_output.png
static/images/bamCoverage_output.png
static/images/bamPEFragmentSize_output.png
static/images/bigwigCompare_output.png
static/images/computeGCBias_output.png
static/images/computeMatrix_advancedOutput.png
static/images/computeMatrix_output.png
static/images/computeMatrix_overview.png
static/images/computeMatrix_selectRegions.png
static/images/computeMatrix_selectScores.png
static/images/multiBamSummary_output.png
static/images/multiBigwigSummary_output.png
static/images/plotCorrelate_RNAseq.png
static/images/plotCorrelation_output.png
static/images/plotCorrelation_scatterplot_PearsonCorr_bigwigScores.png
static/images/plotCoverage_annotated.png
static/images/plotCoverage_output.png
static/images/plotFingerprint_output.png
static/images/plotHeatmap_example.png
static/images/plotHeatmap_example02.png
static/images/plotPCA_annotated.png
static/images/plotProfiler_examples.png
tool_dependencies.xml.orig
removed:
static/images/flowChart_computeMatrixetc.png
b
diff -r 14cb57237e46 -r 275ed3e83de0 computeMatrix.xml
--- a/computeMatrix.xml Mon Jan 25 20:26:32 2016 -0500
+++ b/computeMatrix.xml Mon Feb 15 10:34:00 2016 -0500
[
b'@@ -8,18 +8,25 @@\n     <command>\n <![CDATA[\n         #import tempfile\n+        #set bw_files=[]\n+        #for $counter, $bigwig in enumerate($scoreFileName):\n+            ln -s "${bigwig}" "file_${counter}.bw" &&\n+            #silent $bw_files.append(\'file_%s.bw\' % $counter)\n+        #end for\n+\n+        #set bed_files=[]\n+        #for $counter, $rf in enumerate($regionsFiles):\n+            ln -s "${rf.regionsFile}" "group_${counter}.bed" &&\n+            #silent $bed_files.append(\'group_%s.bed\' % $counter)\n+        #end for\n \n         @BINARY@\n \n             $mode.mode_select\n-            --regionsFileName\n-            #for $rf in $regionsFiles:\n-                \'$rf.regionsFile\' \n-            #end for\n-            --scoreFileName\n-            #for $bw in $scoreFileName:\n-                \'$bw\' \n-            #end for\n+            --regionsFileName \'#echo "\' \'".join($bed_files)#\'\n+\n+            --scoreFileName \'#echo "\' \'".join($bw_files)#\'\n+\n             --outFileName \'$outFileName\'\n \n             @THREADS@\n@@ -51,8 +58,8 @@\n                 --sortRegions \'$advancedOpt.sortRegions\'\n                 --sortUsing \'$advancedOpt.sortUsing\'\n                 --averageTypeBins \'$advancedOpt.averageTypeBins\'\n-                $advancedOpt.skipNAs\n                 $advancedOpt.skipZeros\n+                $advancedOpt.missingDataAsZero\n                 --binSize $advancedOpt.binSize\n \n                 #if $advancedOpt.minThreshold is not None and str($advancedOpt.minThreshold) != \'\':\n@@ -77,17 +84,16 @@\n \n         <param name="scoreFileName" format="bigwig" type="data"\n             label="Score file" multiple="True"\n-            help="You can generate a bigWig file from either a\n-            bedGraph or WIG file using UCSC tools or from a BAM file using the\n+            help="You can generate a bigWig file from a BAM file using the\n             bamCoverage tool. (--scoreFileName)"/>\n \n         <conditional name="mode" >\n             <param name="mode_select" type="select"\n                 label="computeMatrix has two main output options"\n                 help="In the scale-regions mode, all regions in the BED file are\n-                stretched or shrunk to the same length (in bases) that is indicated\n+                stretched or shrunken to the same length (in bases) that is indicated\n                 by the user. Reference-point refers to a position within the BED\n-                regions (e.g start of region). In the reference-point mode only\n+                regions (start or end of each region). In the reference-point mode only\n                 those genomic positions before (upstream) and/or after (downstream)\n                 the reference point will be considered.">\n                 <option value="scale-regions" selected="true">scale-regions</option>\n@@ -164,19 +170,21 @@\n                 </param>\n \n                 <param name="missingDataAsZero" type="boolean" truevalue="--missingDataAsZero" falsevalue="" checked="False"\n-                    label="Convert missing values to 0?."\n-                    help="If set to \'yes\', missing values (NAs) are converted to 0.\n-                          The default is to ignore such cases, which will be\n-                          depicted as black areas once a heatmap is created." />\n+                    label="Convert missing values to 0?"\n+                    help="If set to \'yes\', missing values (NAs) are converted\n+                     to 0. If you want to use clustering with plotHeatmap\n+                     or plotProfile, set this to \'yes\'.\n+                          The default is to ignore missing values, which will be\n+                          depicted as black areas once a heatmap is created.\n+                           (--missingDataAsZero)" />\n \n                 <expand macro="skipZeros" />\n-                <expand macro="skipNAs" />\n \n                 <param name="minThreshold" type="float" optional="True"\n                     label="Minimum threshold"\n                     help="'..b'\n-Genomic regions can really be anything - genes, parts of genes, ChIP-seq\n-peaks, favorite genome regions... as long as you provide a proper file\n-in BED or INTERVAL format. If you would like to compare different groups of regions\n-(i.e. genes from chromosome 2 and 3), you can supply more than 1 BED file, one for each group.\n+The supplied genomic regions can really be anything - genes, parts of genes, ChIP-seq peaks, favorite genome regions... as long as you provide a proper file\n+in BED or INTERVAL format. If you would like to compare different groups of regions (e.g., genes from chromosome 2 and 3), you can supply more than 1 regions file, one for each group by selecting "Insert Select regions".\n+\n+.. image:: $PATH_TO_IMAGES/computeMatrix_selectRegions.png\n+   :width: 600\n+   :height: 150\n+\n+You can select as many score (bigWig) files as you like. Simply use the Shift and/or Command key while clicking on the files of interest.\n \n-computeMatrix can also be used to filter and sort\n-regions according to their score by making use of its advanced output options.\n+.. image:: $PATH_TO_IMAGES/computeMatrix_selectScores.png\n+   :width: 600\n+   :height: 136\n+\n+The multitude of parameters can seem daunting at first - here are the options that we tend to tune most often:\n+\n+* ``bin Size`` -- The default value works well most of the time, but if you want to have a more finely grained image, decrease the default value (but not smaller than your bigWig file(s)\' bin size). If you want to reduce the computation time, increase it.\n+* ``Skip zeros`` -- useful to avoid completely blank lines in the heatmap.\n+* ``Convert missing values to 0?`` -- If you want to identify clusters of similar regions in an unsupervised fashion using ``plotHeatmap`` and/or ``plotProfile``, you should definitely set this to \'yes\'.\n \n \n-.. image:: $PATH_TO_IMAGES/flowChart_computeMatrixetc.png\n-   :alt: Relationship between computeMatrix, heatmapper and profiler\n+Output files\n+---------------\n+\n+The default output is a **gzipped table of values** that is used by both ``plotHeatmap`` and ``plotProfile``.\n+\n+The optional output files include a) the **regions after sorting and filtering (if selected)** as they were used to calculate the values for the plotting, and b) the uncompressed table that **underlies the heatmap**.\n+\n+**TIP:** ``computeMatrix`` can also be used to filter and sort regions according to their score by making use of the "advanced output settings".\n+\n+.. image:: $PATH_TO_IMAGES/computeMatrix_advancedOutput.png\n+   :width: 600\n+   :height: 189\n \n+.. image:: $PATH_TO_IMAGES/computeMatrix_output.png\n+   :width: 600\n+   :height: 297\n \n-You can find more details on the computeMatrix doc page: https://deeptools.readthedocs.org/en/master/content/tools/computeMatrix.html\n+Note that these advanced output options are available for ``plotHeatmap`` and ``plotProfile``, too.\n+\n+See the following table for the optional output options:\n \n++-----------------------------------+--------------------+-----------------+-----------------+\n+|  **optional output type**         |  **computeMatrix** | **plotHeatmap** | **plotProfile** |\n++-----------------------------------+--------------------+-----------------+-----------------+\n+| values underlying the heatmap     |  yes               | yes             | no              |\n++-----------------------------------+--------------------+-----------------+-----------------+\n+| values underlying the profile     | no                 | no              | yes             |\n++-----------------------------------+--------------------+-----------------+-----------------+\n+| sorted and/or filtered regions    | yes                | yes             | yes             |\n++-----------------------------------+--------------------+-----------------+-----------------+\n+\n+**More examples** can be found in our `Gallery <http://deeptools.readthedocs.org/en/latest/content/example_gallery.html#normalized-chip-seq-signals-and-peak-regions>`_.\n \n -----\n \n'
b
diff -r 14cb57237e46 -r 275ed3e83de0 deepTools_macros.xml
--- a/deepTools_macros.xml Mon Jan 25 20:26:32 2016 -0500
+++ b/deepTools_macros.xml Mon Feb 15 10:34:00 2016 -0500
[
@@ -72,12 +72,12 @@
     </xml>
 
     <token name="@THREADS@">--numberOfProcessors "\${GALAXY_SLOTS:-4}"</token>
-    <token name="@WRAPPER_VERSION@">2.0.1</token>
+    <token name="@WRAPPER_VERSION@">2.1.0</token>
     <xml name="requirements">
         <requirements>
             <requirement type="package" version="2.7.10">python</requirement>
             <requirement type="binary">@BINARY@</requirement>
-            <requirement type="package" version="2.0.1">deepTools</requirement>
+            <requirement type="package" version="2.1.0">deepTools</requirement>
             <yield />
         </requirements>
         <expand macro="stdio" />
@@ -104,6 +104,7 @@
                     <param name="clustering_options" type="select" label="Clustering algorithm">
                         <option value="none">No clustering</option>
                         <option value="kmeans">Kmeans clustering</option>
+                        <option value="hclust">Hierarchical clustering</option>
                     </param>
                     <when value="kmeans">
                         <param name="k_kmeans" type="integer" value="0" label="Number of clusters to compute"
@@ -112,6 +113,15 @@
                             If more specific clustering methods are required it is advisable to save the underlying matrix and
                             run the clustering using other software."/>
                     </when>
+                    <when value="hclust">
+                        <param name="n_hclust" type="integer" value="0" label="number of clusters to compute."
+                            help="WARNING: This option causes the tool to run for a very long time! When this option is
+                            set, the matrix is split into clusters using the hierarchical clustering algorithm, using ward linkage.
+                            This only works for data that is not grouped, otherwise only the first group will be clustered.
+                            Note that you must have used the '--missingDataAsZero' option within computeMatrix!
+                            If more specific clustering methods are required it is advisable to save the underlying matrix and
+                            run the clustering using other software."/>
+                    </when>
                     <when value="none" />
                 </conditional>
             </when>
@@ -126,6 +136,11 @@
                     --kmeans $advancedOpt.used_multiple_regions.clustering.k_kmeans
                 #end if
             #end if
+            #if $advancedOpt.used_multiple_regions.clustering.clustering_options == 'hclust':
+                #if int($advancedOpt.used_multiple_regions.clustering.n_hclust) > 0:
+                    --hclust $advancedOpt.used_multiple_regions.clustering.n_hclust
+                #end if
+            #end if
         #end if
     </token>
 
@@ -293,6 +308,12 @@
     </xml>
 
     <xml name="multiple_input_bams">
+        <param argument="--bamfiles" type="data" format="bam" min="1"
+            label="Bam file" multiple="true"
+            help="The BAM file must be sorted."/>
+    </xml>
+
+    <xml name="multiple_input_bams_min2">
         <param argument="--bamfiles" type="data" format="bam" min="2"
             label="Bam file" multiple="true"
             help="The BAM file must be sorted."/>
@@ -310,6 +331,7 @@
             help="Title of the plot, to be printed on top of the generated image." />
     </xml>
 
+
     <token name="@multiple_input_bams@">
 <![CDATA[
         #set files=[]
b
diff -r 14cb57237e46 -r 275ed3e83de0 deepTools_macros.xml.orig
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/deepTools_macros.xml.orig Mon Feb 15 10:34:00 2016 -0500
b
b'@@ -0,0 +1,639 @@\n+<macros>\n+\n+    <xml name="advancedOpt_scaffold">\n+        <conditional name="advancedOpt">\n+            <param name="showAdvancedOpt" type="select" label="Show advanced options" >\n+                <option value="no" selected="true">no</option>\n+                <option value="yes">yes</option>\n+            </param>\n+            <when value="no" />\n+            <when value="yes">\n+                <yield/>\n+            </when>\n+        </conditional>\n+    </xml>\n+\n+    <token name="@ADVANCED_OPTS_READ_PROCESSING@">\n+        #if $advancedOpt.doExtendCustom.doExtend == \'custom\':\n+            --extendReads $advancedOpt.doExtendCustom.extendReadsValue\n+        #else if $advancedOpt.doExtendCustom.doExtend == \'yes\':\n+            --extendReads\n+        #end if\n+        $advancedOpt.ignoreDuplicates\n+        $advancedOpt.centerReads\n+        #if $advancedOpt.minMappingQuality:\n+            --minMappingQuality \'$advancedOpt.minMappingQuality\'\n+        #end if\n+        #if $advancedOpt.samFlagInclude:\n+            --samFlagInclude $advancedOpt.samFlagInclude\n+        #end if\n+        #if $advancedOpt.samFlagExclude:\n+            --samFlagExclude $advancedOpt.samFlagExclude\n+        #end if\n+    </token>\n+\n+    <xml name="heatmap_options">\n+        <expand macro="zMin_zMax" />\n+        <expand macro="colorMap" />\n+        <expand macro="plotTitle" />\n+        <expand macro="plotNumbers" />\n+    </xml>\n+\n+    <token name="@HEATMAP_OPTIONS@">\n+        #if str($plotting_type.zMin) != "":\n+            --zMin $plotting_type.zMin\n+        #end if\n+        #if str($plotting_type.zMax) != "":\n+            --zMax $plotting_type.zMax\n+        #end if\n+        --colorMap \'$plotting_type.colorMap\'\n+        $plotting_type.plotNumbers\n+        --plotTitle \'$plotting_type.plotTitle\'\n+    </token>\n+\n+\n+    <xml name="includeZeros">\n+        <param argument="--includeZeros" type="boolean" truevalue="--includeZeros" falsevalue=""\n+            label="Include zeros"\n+            help="If set, then regions with zero counts for *all* BAM files are included. The default behavior is to ignore such regions." />\n+    </xml>\n+\n+    <xml name="zMin_zMax">\n+        <param argument="--zMin" type="float" value="" optional="true" label="Minimum value for the heatmap intensities"\n+            help="If not specified the value is set automatically."/>\n+        <param argument="--zMax" type="float" value="" optional="true" label="Maximum value for the heatmap intensities"\n+            help="If not specified the value is set automatically."/>\n+    </xml>\n+\n+    <xml name="region_limit_operation">\n+        <param argument="--region" type="text" value=""\n+            label="Region of the genome to limit the operation to"\n+            help="This is useful when testing parameters to reduce the time required. The format is chr:start:end, for example &quot;chr10&quot; or &quot;chr10:456700:891000&quot;." />\n+    </xml>\n+\n+    <token name="@THREADS@">--numberOfProcessors "\\${GALAXY_SLOTS:-4}"</token>\n+<<<<<<< HEAD\n+    <token name="@WRAPPER_VERSION@">2.0.1.0</token>\n+=======\n+    <token name="@WRAPPER_VERSION@">2.1.0</token>\n+>>>>>>> d56067f86089e75354a2fad266002db3730766d9\n+    <xml name="requirements">\n+        <requirements>\n+            <requirement type="package" version="2.7.10">python</requirement>\n+            <requirement type="binary">@BINARY@</requirement>\n+<<<<<<< HEAD\n+            <requirement type="package" version="2.0.1">deepTools</requirement>\n+=======\n+            <requirement type="package" version="2.1.0">deepTools</requirement>\n+>>>>>>> d56067f86089e75354a2fad266002db3730766d9\n+            <yield />\n+        </requirements>\n+        <expand macro="stdio" />\n+        <version_command>@BINARY@ --version</version_command>\n+    </xml>\n+\n+    <xml name="smoothLength">\n+        <param argument="--smoothLength" type="integer" value="" optional="True" min="1"\n+            label="Smooth values using the following length (in bases)"\n+            help '..b'k2 reversed</option>\n+            <option value="Reds_r">Reds reversed</option>\n+            <option value="Oranges_r">Oranges reversed</option>\n+            <option value="Greens_r">Greens reversed</option>\n+            <option value="Blues_r">Blues reversed</option>\n+            <option value="Greys_r">Greys reversed</option>\n+            <option value="Purples_r">Purples reversed</option>\n+            <option value="Paired_r">Paired reversed</option>\n+            <option value="Pastel1_r">Pastel1 reversed</option>\n+            <option value="Pastel2_r">Pastel2 reversed</option>\n+            <option value="spring_r">spring reversed</option>\n+            <option value="summer_r">summer reversed</option>\n+            <option value="autumn_r">autumn reversed</option>\n+            <option value="winter_r">winter reversed</option>\n+            <option value="hot_r">hot reversed</option>\n+            <option value="coolwarm_r">coolwarm reversed</option>\n+            <option value="cool_r">cool reversed</option>\n+            <option value="seismic_r">seismic reversed</option>\n+            <option value="terrain_r">terrain reversed</option>\n+            <option value="ocean_r">ocean reversed</option>\n+            <option value="rainbow_r">rainbow reversed</option>\n+            <option value="bone_r">bone reversed</option>\n+            <option value="flag_r">flag reversed</option>\n+            <option value="prism_r">prism reversed</option>\n+            <option value="cubehelix_r">cubehelix reversed</option>\n+            <option value="binary_r">binary reversed</option>\n+            <option value="pink_r">pink reversed</option>\n+            <option value="gray_r">gray reversed</option>\n+            <option value="copper_r">copper reversed</option>\n+            <option value="BrBG_r">BrBG reversed</option>\n+            <option value="BuGn_r">BuGn reversed</option>\n+            <option value="BuPu_r">BuPu reversed</option>\n+            <option value="GnBu_r">GnBu reversed</option>\n+            <option value="OrRd_r">OrRd reversed</option>\n+            <option value="PiYG_r">PiYG reversed</option>\n+            <option value="PRGn_r">PRGn reversed</option>\n+            <option value="PuOr_r">PuOr reversed</option>\n+            <option value="PuRd_r">PuRd reversed</option>\n+            <option value="PuBu_r">PuBu reversed</option>\n+            <option value="RdBu_r">RdBu reversed</option>\n+            <option value="RdGy_r">RdGy reversed</option>\n+            <option value="RdPu_r">RdPu reversed</option>\n+            <option value="YlGn_r">YlGn reversed</option>\n+            <option value="PuBuGn_r">PuBuGn reversed</option>\n+            <option value="RdYlBu_r">RdYlBu reversed</option>\n+            <option value="RdYlGn_r">RdYlGn reversed</option>\n+            <option value="YlGnBu_r">YlGnBu reversed</option>\n+            <option value="YlOrBr_r">YlOrBr reversed</option>\n+            <option value="YlOrRd_r">YlOrRd reversed</option>\n+            <option value="gist_gray_r">gist_gray reversed</option>\n+            <option value="gist_stern_r">gist_stern reversed</option>\n+            <option value="gist_earth_r">gist_earth reversed</option>\n+            <option value="gist_yarg_r">gist_yarg reversed</option>\n+            <option value="gist_ncar_r">gist_ncar reversed</option>\n+            <option value="gist_rainbow_r">gist_rainbow reversed</option>\n+            <option value="gist_heat_r">gist_heat reversed</option>\n+            <option value="gnuplot_r">gnuplot reversed</option>\n+            <option value="gnuplot2_r">gnuplot2 reversed</option>\n+            <option value="CMRmap_r">CMRmap reversed</option>\n+            <option value="bwr_r">bwr reversed</option>\n+            <option value="hsv_r">hsv reversed</option>\n+            <option value="brg_r">brg reversed</option>\n+            <option value="jet_r">jet reversed</option>\n+            <option value="afmhot_r">afmhot reversed</option>\n+        </param>\n+\n+    </xml>\n+\n+</macros>\n'
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diff -r 14cb57237e46 -r 275ed3e83de0 test-data/bamPEFragmentSize_result1.txt
--- a/test-data/bamPEFragmentSize_result1.txt Mon Jan 25 20:26:32 2016 -0500
+++ b/test-data/bamPEFragmentSize_result1.txt Mon Feb 15 10:34:00 2016 -0500
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@@ -2,19 +2,19 @@
 
 
 Fragment lengths:
-Min.: 241
+Min.: 241.0
 1st Qu.: 241.5
 Mean: 244.666666667
 Median: 242.0
 3rd Qu.: 246.5
-Max.: 251
+Max.: 251.0
 Std: 4.49691252108
 
 Read lengths:
-Min.: 251
+Min.: 251.0
 1st Qu.: 251.0
 Mean: 251.0
 Median: 251.0
 3rd Qu.: 251.0
-Max.: 251
+Max.: 251.0
 Std: 0.0
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diff -r 14cb57237e46 -r 275ed3e83de0 test-data/plotCoverage_result1.tabular
--- a/test-data/plotCoverage_result1.tabular Mon Jan 25 20:26:32 2016 -0500
+++ b/test-data/plotCoverage_result1.tabular Mon Feb 15 10:34:00 2016 -0500
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0\n+chrM\t16564\t16565\t0.0\t0.0\n+chrM\t16565\t16566\t0.0\t0.0\n+chrM\t16566\t16567\t0.0\t0.0\n+chrM\t16567\t16568\t0.0\t0.0\n+chrM\t16568\t16569\t0.0\t0.0\n'
b
diff -r 14cb57237e46 -r 275ed3e83de0 tool_dependencies.xml
--- a/tool_dependencies.xml Mon Jan 25 20:26:32 2016 -0500
+++ b/tool_dependencies.xml Mon Feb 15 10:34:00 2016 -0500
b
@@ -1,9 +1,9 @@
 <?xml version="1.0"?>
 <tool_dependency>
     <package name="python" version="2.7.10">
-        <repository changeset_revision="0339c4a9b87b" name="package_python_2_7_10" owner="iuc" toolshed="https://toolshed.g2.bx.psu.edu" />
+        <repository changeset_revision="0339c4a9b87b" name="package_python_2_7_10" owner="iuc" prior_installation_required="True" toolshed="https://toolshed.g2.bx.psu.edu" />
     </package>
-    <package name="deepTools" version="2.0.1">
-        <repository changeset_revision="1fb61fa32733" name="package_python_2_7_deeptools_2_0_1" owner="iuc" toolshed="https://toolshed.g2.bx.psu.edu" />
+    <package name="deepTools" version="2.1.0">
+        <repository changeset_revision="2c54c7c38299" name="package_python_2_7_deeptools_2_1_0" owner="iuc" toolshed="https://toolshed.g2.bx.psu.edu" />
     </package>
 </tool_dependency>
b
diff -r 14cb57237e46 -r 275ed3e83de0 tool_dependencies.xml.orig
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/tool_dependencies.xml.orig Mon Feb 15 10:34:00 2016 -0500
[
@@ -0,0 +1,76 @@
+<?xml version='1.0' encoding='utf-8'?>
+<tool_dependency>
+    <package name="numpy" version="1.9">
+        <repository name="package_python_2_7_numpy_1_9" owner="iuc" prior_installation_required="True" />
+    </package>
+    <package name="pybigwig" version="0.2.4">
+        <repository name="package_python_2_7_10_pybigwig_0_2_4" owner="iuc" prior_installation_required="True"/>
+    </package>
+    <package name="matplotlib" version="1.4">
+        <repository name="package_python_2_7_matplotlib_1_4" owner="iuc" prior_installation_required="True" />
+    </package>
+    <package name="scipy" version="0.14">
+        <repository name="package_python_2_7_scipy_0_14" owner="iuc" prior_installation_required="True" />
+    </package>
+<<<<<<< HEAD
+    <package name="deepTools" version="2.0.1">
+        <repository name="package_python_2_7_deeptools_2_0_1" owner="iuc"/>
+=======
+    <package name="pysam" version="0.8.3">
+        <repository name="package_python_2_7_pysam_0_8_3" owner="iuc" prior_installation_required="True" />
+    </package>
+    <package name="bx-python" version="0.7.2">
+        <repository name="package_python_2_7_bx_python_0_7" owner="iuc" prior_installation_required="True" />
+    </package>
+    <package name="python" version="2.7.10">
+        <repository name="package_python_2_7_10" owner="iuc" prior_installation_required="True" />
+>>>>>>> d56067f86089e75354a2fad266002db3730766d9
+    </package>
+    <package name="deepTools" version="2.1.0">
+        <install version="1.0">
+            <actions>
+                <action type="setup_python_environment">
+                    <repository name="package_python_2_7_10" owner="iuc">
+                        <package name="python" version="2.7.10" />
+                    </repository>
+                    <repository name="package_python_2_7_pysam_0_8_3" owner="iuc">
+                        <package name="pysam" version="0.8.3" />
+                    </repository>
+                    <repository name="package_python_2_7_numpy_1_9" owner="iuc">
+                        <package name="numpy" version="1.9" />
+                    </repository>
+                    <repository name="package_python_2_7_matplotlib_1_4" owner="iuc">
+                        <package name="matplotlib" version="1.4" />
+                    </repository>
+                    <repository name="package_python_2_7_scipy_0_14" owner="iuc">
+                        <package name="scipy" version="0.14" />
+                    </repository>
+                    <repository name="package_python_2_7_bx_python_0_7" owner="iuc">
+                        <package name="bx-python" version="0.7.2" />
+                    </repository>
+                    <repository name="package_python_2_7_10_pybigwig_0_2_4" owner="iuc">
+                        <package name="pybigwig" version="0.2.4" />
+                    </repository>
+                    <package sha256sum="3bf835afde02cd5247198db3e01f690899e1fdb86e1821a83ac940d807baa857">https://pypi.python.org/packages/source/d/deepTools/deepTools-2.1.0.tar.gz</package>
+                </action>
+
+                <action type="set_environment">
+                    <environment_variable name="PATH" action="prepend_to">$INSTALL_DIR/bin</environment_variable>
+                    <environment_variable name="PYTHONPATH" action="prepend_to">$INSTALL_DIR</environment_variable>
+                    <environment_variable name="DEEPTOOLS_ROOT_PATH" action="set_to">$INSTALL_DIR</environment_variable>
+                    <environment_variable name="DEEPTOOLS_PYTHONPATH" action="set_to">$INSTALL_DIR</environment_variable>
+                    <environment_variable name="DEEPTOOLS_PATH" action="set_to">$INSTALL_DIR/bin</environment_variable>
+                    <!-- libpng lib path -->
+                    <environment_variable action="set_to" name="LIBPNG_LIB_PATH">$ENV[LIBPNG_LIB_PATH]</environment_variable>
+                    <environment_variable action="prepend_to" name="LD_LIBRARY_PATH">$ENV[LIBPNG_LIB_PATH]</environment_variable>
+                    <!-- disable the config file of deepTools -->
+                    <environment_variable name="DEEP_TOOLS_NO_CONFIG" action="set_to">TRUE</environment_variable>
+                </action>
+             </actions>
+         </install>
+         <readme>
+            Installation of deepTools from Fidel Ramirez.
+            https://github.com/fidelram/deepTools
+         </readme>
+     </package>
+</tool_dependency>