Previous changeset 16:81e4014fa809 (2022-08-19) Next changeset 18:768d04526638 (2022-09-26) |
Commit message:
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit db275932cbb485cb44ae91e0b421d6f57698db49 |
modified:
bcftools_isec.xml macros.xml test-data/summary.pdf |
added:
test-data/mpileup.AD.vcf |
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diff -r 81e4014fa809 -r 5f27e814a119 bcftools_isec.xml --- a/bcftools_isec.xml Fri Aug 19 19:53:22 2022 +0000 +++ b/bcftools_isec.xml Tue Sep 20 12:52:08 2022 +0000 |
[ |
@@ -1,5 +1,5 @@ <?xml version='1.0' encoding='utf-8'?> -<tool name="bcftools @EXECUTABLE@" id="bcftools_@EXECUTABLE@" version="@TOOL_VERSION@+galaxy1"> +<tool name="bcftools @EXECUTABLE@" id="bcftools_@EXECUTABLE@" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@"> <description>Create intersections, unions and complements of VCF files</description> <macros> <token name="@EXECUTABLE@">isec</token> @@ -83,7 +83,25 @@ </assert_contents> </output> </test> - + <!-- Test region overlap option --> + <test> + <param name="input_files" ftype="vcf" value="isec.b.vcf,isec.a.vcf" /> + <param name="nfiles" value="=2" /> + <param name="output_type" value="v" /> + <section name="sec_restrict"> + <param name="regions_overlap" value="1"/> + </section> + <output name="output_file"> + <assert_contents> + <has_text text="3157410" /> + <has_text text="3162006" /> + <not_has_text text="3062915" /> + </assert_contents> + </output> + <assert_command> + <has_text text="--regions-overlap" /> + </assert_command> + </test> </tests> <help><![CDATA[ ===================================== |
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diff -r 81e4014fa809 -r 5f27e814a119 macros.xml --- a/macros.xml Fri Aug 19 19:53:22 2022 +0000 +++ b/macros.xml Tue Sep 20 12:52:08 2022 +0000 |
[ |
@@ -1,5 +1,7 @@ <macros> - <token name="@TOOL_VERSION@">1.10</token> + <token name="@TOOL_VERSION@">1.15.1</token> + <token name="@VERSION_SUFFIX@">0</token> + <token name="@PROFILE@">20.01</token> <xml name="bio_tools"> <xrefs> <xref type="bio.tools">bcftools</xref> @@ -8,15 +10,15 @@ <xml name="requirements"> <requirements> <requirement type="package" version="@TOOL_VERSION@">bcftools</requirement> - <requirement type="package" version="1.10">htslib</requirement> + <requirement type="package" version="1.15.1">htslib</requirement> <yield /> </requirements> </xml> <xml name="samtools_requirement"> - <requirement type="package" version="1.10">samtools</requirement> + <requirement type="package" version="1.15.1">samtools</requirement> </xml> <xml name="matplotlib_requirement"> - <requirement type="package" version="3.4.3">matplotlib</requirement> + <requirement type="package" version="3.5.3">matplotlib</requirement> </xml> <xml name="version_command"> <version_command>bcftools 2>&1 | grep 'Version:'</version_command> @@ -39,6 +41,10 @@ ]]> </token> <xml name="macro_input"> + <!-- + REQUIRES https://github.com/galaxyproject/galaxy/pull/14605/files + <param name="input_files" type="data" format="vcf,vcf.gz,vcf_bgzip,bcf" label="Other VCF/BCF Datasets" multiple="True" /> + --> <param name="input_file" type="data" format="vcf,vcf_bgzip,bcf" label="VCF/BCF Data" /> </xml> <token name="@PREPARE_INPUT_FILE@"> @@ -48,6 +54,7 @@ #if $input_file.is_of_type('vcf') bgzip -c '$input_file' > $input_vcf && bcftools index $input_vcf && +##elif $input_file.is_of_type('vcf_bgzip') or $input_file.is_of_type('vcf.gz') #elif $input_file.is_of_type('vcf_bgzip') ln -s '$input_file' $input_vcf && #if $input_file.metadata.tabix_index: @@ -71,6 +78,10 @@ </token> <xml name="macro_inputs"> + <!-- + REQUIRES https://github.com/galaxyproject/galaxy/pull/14605/files + <param name="input_files" type="data" format="vcf,vcf.gz,vcf_bgzip,bcf" label="Other VCF/BCF Datasets" multiple="True" /> + --> <param name="input_files" type="data" format="vcf,vcf_bgzip,bcf" label="Other VCF/BCF Datasets" multiple="True" /> </xml> <token name="@PREPARE_INPUT_FILES@"> @@ -83,7 +94,8 @@ #if $input_file.is_of_type('vcf') bgzip -c '$input_file' > $input_vcf && bcftools index $input_vcf && - #elif $input_file.is_of_type('vcf_bgzip') + ##elif $input_file.is_of_type('vcf_bgzip') or $input_file.is_of_type('vcf.gz') -> REQUIRES https://github.com/galaxyproject/galaxy/pull/14605 + #elif $input_file.is_of_type('vcf_bgzip') or $input_file.is_of_type('vcf.gz') ln -s '$input_file' $input_vcf && #if $input_file.metadata.tabix_index: ln -s '${input_file.metadata.tabix_index}' ${input_vcf}.tbi && @@ -318,6 +330,11 @@ <yield /> </when> </conditional> + <param argument="--@TYPE@s-overlap" type="select" optional="true" label="@LABEL_TYPE@ overlap" help="Include if POS in the region (0), record overlaps (1), variant overlaps (2)"> + <option value="0">0: POS in the region</option> + <option value="1">1: Record overlaps</option> + <option value="2">2: Variant overlaps</option> + </param> </xml> <token name="@PARSE_INTERVALS@"> @@ -337,6 +354,28 @@ ]]> </token> + <token name="@MASK@"> +<![CDATA[ +#if $section.conditional_soft_filter.selector == 'enabled' and $section.conditional_soft_filter.soft_filter + #if $section.conditional_soft_filter.masks.masks_src == 'regions': + #set $intervals = $section.conditional_soft_filter.masks.masks + @PARSE_INTERVALS@ + --mask '$intervals_spec' + #elif $section.conditional_soft_filter.masks.masks_src == 'masks_file' and $section.conditional_soft_filter.masks.masks_file: + #if $masks_path is not None: + --mask-file '$masks_path' + #else: + --mask-file '$section.conditional_soft_filter.masks.masks_file' + #end if + #end if + #if $section.conditional_soft_filter.masks_overlap + --mask-overlap $section.conditional_soft_filter.masks_overlap + #end if +#end if + +]]> + </token> + <token name="@REGIONS@"> <![CDATA[ #if $section.regions.regions_src == 'regions': @@ -350,6 +389,10 @@ --regions-file '$section.regions.regions_file' #end if #end if +#if $section.regions_overlap + --regions-overlap $section.regions_overlap +#end if + ]]> </token> @@ -364,6 +407,9 @@ #elif $section.targets.targets_src == 'targets_file' and $section.targets.targets_file: --targets-file "${section.targets.invert_targets_file}${section.targets.targets_file}" #end if +#if $section.targets_overlap + --targets-overlap $section.targets_overlap +#end if ]]> </token> @@ -410,8 +456,8 @@ <xml name="macro_samples"> <param argument="--samples" type="text" value="" optional="true" label="Samples" - help="Comma separated list of samples to annotate (or exclude)"> - <validator type="regex" message="">^(\w+(,\w+)*)?$</validator> + help="Comma-separated list of samples to annotate (or exclude) or - to include all samples"> + <validator type="regex" message="Comma-separated list of samples or - to include all samples">^(-|\w+(,\w+)*)?$</validator> </param> <param name="invert_samples" type="boolean" truevalue="^" falsevalue="" checked="false" label="Invert Samples" help="Inverts the query/filtering applied by Samples (adds "^" prefix to exclude)" /> @@ -476,7 +522,7 @@ <xml name="macro_columns"> <param name="columns" type="text" value="" optional="true" label="Columns" help="List of columns in the annotation file, e.g. CHROM,POS,REF,ALT,-,INFO/TAG. See man page for details"> - <validator type="regex" message="COLUMN names separated by commas">^([^,]+(,[^,]+)*)?$</validator> + <validator type="regex" message="COLUMN names separated by commas">^([^,]+(,[^,]+)*)?$</validator> </param> </xml> <token name="@COLUMNS@"> @@ -496,7 +542,18 @@ ${section.vcf_ids} </token> +<xml name="macro_output_tags"> + <param name="output_tags" argument="--annotate" type="select" optional="true" multiple="True" display="checkboxes" label="Optional tags to output" help="--annotate"> + <yield /> + </param> +</xml> + +<xml name="macro_overlap" token_argument="" token_label=""> +</xml> + + <token name="@OUTPUT_HELP@"> + <![CDATA[ Output Type ----------- |
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diff -r 81e4014fa809 -r 5f27e814a119 test-data/mpileup.AD.vcf --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/mpileup.AD.vcf Tue Sep 20 12:52:08 2022 +0000 |
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b'@@ -0,0 +1,4131 @@\n+##fileformat=VCFv4.2\n+##FILTER=<ID=PASS,Description="All filters passed">\n+##bcftoolsVersion=1.11-21-g0987715+htslib-1.11-3-g450c912\n+##bcftoolsCommand=mpileup -O v -a AD,DP,INFO/ADR,INFO/ADF,DP4,INFO/DPR -f xxx//mpileup.ref.fa -b xxx//bamlist.txt\n+##reference=file://mpileup.ref.fa\n+##contig=<ID=17,length=4200>\n+##ALT=<ID=*,Description="Represents allele(s) other than observed.">\n+##INFO=<ID=INDEL,Number=0,Type=Flag,Description="Indicates that the variant is an INDEL.">\n+##INFO=<ID=IDV,Number=1,Type=Integer,Description="Maximum number of raw reads supporting an indel">\n+##INFO=<ID=IMF,Number=1,Type=Float,Description="Maximum fraction of raw reads supporting an indel">\n+##INFO=<ID=DP,Number=1,Type=Integer,Description="Raw read depth">\n+##INFO=<ID=VDB,Number=1,Type=Float,Description="Variant Distance Bias for filtering splice-site artefacts in RNA-seq data (bigger is better)",Version="3">\n+##INFO=<ID=RPB,Number=1,Type=Float,Description="Mann-Whitney U test of Read Position Bias (bigger is better)">\n+##INFO=<ID=MQB,Number=1,Type=Float,Description="Mann-Whitney U test of Mapping Quality Bias (bigger is better)">\n+##INFO=<ID=BQB,Number=1,Type=Float,Description="Mann-Whitney U test of Base Quality Bias (bigger is better)">\n+##INFO=<ID=MQSB,Number=1,Type=Float,Description="Mann-Whitney U test of Mapping Quality vs Strand Bias (bigger is better)">\n+##INFO=<ID=SGB,Number=1,Type=Float,Description="Segregation based metric.">\n+##INFO=<ID=MQ0F,Number=1,Type=Float,Description="Fraction of MQ0 reads (smaller is better)">\n+##INFO=<ID=I16,Number=16,Type=Float,Description="Auxiliary tag used for calling, see description of bcf_callret1_t in bam2bcf.h">\n+##INFO=<ID=QS,Number=R,Type=Float,Description="Auxiliary tag used for calling">\n+##FORMAT=<ID=PL,Number=G,Type=Integer,Description="List of Phred-scaled genotype likelihoods">\n+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Number of high-quality bases">\n+##INFO=<ID=DPR,Number=R,Type=Integer,Description="Number of high-quality bases observed for each allele">\n+##FORMAT=<ID=DP4,Number=4,Type=Integer,Description="Number of high-quality ref-fwd, ref-reverse, alt-fwd and alt-reverse bases">\n+##FORMAT=<ID=AD,Number=R,Type=Integer,Description="Allelic depths (high-quality bases)">\n+##INFO=<ID=ADF,Number=R,Type=Integer,Description="Total allelic depths on the forward strand (high-quality bases)">\n+##INFO=<ID=ADR,Number=R,Type=Integer,Description="Total allelic depths on the reverse strand (high-quality bases)">\n+#CHROM\tPOS\tID\tREF\tALT\tQUAL\tFILTER\tINFO\tFORMAT\tHG00100\tHG00101\tHG00102\tHG99999\n+17\t1\t.\tA\t<*>\t0\t.\tDP=14;ADF=0,0;ADR=0,0;DPR=0,0;I16=0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0;QS=0,0;MQ0F=0\tPL:DP:DP4:AD\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\n+17\t2\t.\tA\t<*>\t0\t.\tDP=14;ADF=0,0;ADR=0,0;DPR=0,0;I16=0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0;QS=0,0;MQ0F=0\tPL:DP:DP4:AD\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\n+17\t3\t.\tG\t<*>\t0\t.\tDP=14;ADF=0,0;ADR=0,0;DPR=0,0;I16=0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0;QS=0,0;MQ0F=0\tPL:DP:DP4:AD\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\n+17\t4\t.\tC\t<*>\t0\t.\tDP=14;ADF=0,0;ADR=0,0;DPR=0,0;I16=0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0;QS=0,0;MQ0F=0\tPL:DP:DP4:AD\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\n+17\t5\t.\tT\t<*>\t0\t.\tDP=14;ADF=0,0;ADR=0,0;DPR=0,0;I16=0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0;QS=0,0;MQ0F=0\tPL:DP:DP4:AD\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\n+17\t6\t.\tT\t<*>\t0\t.\tDP=14;ADF=0,0;ADR=0,0;DPR=0,0;I16=0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0;QS=0,0;MQ0F=0\tPL:DP:DP4:AD\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\n+17\t7\t.\tC\t<*>\t0\t.\tDP=14;ADF=0,0;ADR=0,0;DPR=0,0;I16=0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0;QS=0,0;MQ0F=0\tPL:DP:DP4:AD\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\n+17\t8\t.\tT\t<*>\t0\t.\tDP=14;ADF=0,0;ADR=0,0;DPR=0,0;I16=0,0,0,0,0,0,0,0,0,0,0,0,0,'..b'L:DP:DP4:AD\t0,9,103:3:2,1,0,0:3,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\n+17\t4083\t.\tC\t<*>\t0\t.\tDP=3;ADF=2,0;ADR=1,0;DPR=3,0;I16=2,1,0,0,104,3648,0,0,134,6338,0,0,22,340,0,0;QS=1,0;MQSB=1;MQ0F=0\tPL:DP:DP4:AD\t0,9,98:3:2,1,0,0:3,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\n+17\t4084\t.\tA\t<*>\t0\t.\tDP=2;ADF=1,0;ADR=1,0;DPR=2,0;I16=1,1,0,0,78,3050,0,0,97,4969,0,0,20,298,0,0;QS=1,0;MQSB=1;MQ0F=0\tPL:DP:DP4:AD\t0,6,74:2:1,1,0,0:2,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\n+17\t4085\t.\tC\t<*>\t0\t.\tDP=2;ADF=1,0;ADR=1,0;DPR=2,0;I16=1,1,0,0,62,1940,0,0,97,4969,0,0,18,260,0,0;QS=1,0;MQSB=1;MQ0F=0\tPL:DP:DP4:AD\t0,6,62:2:1,1,0,0:2,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\n+17\t4086\t.\tG\t<*>\t0\t.\tDP=2;ADF=1,0;ADR=1,0;DPR=2,0;I16=1,1,0,0,56,1640,0,0,97,4969,0,0,16,226,0,0;QS=1,0;MQSB=1;MQ0F=0\tPL:DP:DP4:AD\t0,6,56:2:1,1,0,0:2,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\n+17\t4087\t.\tC\t<*>\t0\t.\tDP=2;ADF=1,0;ADR=1,0;DPR=2,0;I16=1,1,0,0,69,2405,0,0,97,4969,0,0,14,196,0,0;QS=1,0;MQSB=1;MQ0F=0\tPL:DP:DP4:AD\t0,6,68:2:1,1,0,0:2,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\n+17\t4088\t.\tA\t<*>\t0\t.\tDP=1;ADF=1,0;ADR=0,0;DPR=1,0;I16=1,0,0,0,39,1521,0,0,37,1369,0,0,13,169,0,0;QS=1,0;MQ0F=0\tPL:DP:DP4:AD\t0,3,37:1:1,0,0,0:1,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\n+17\t4089\t.\tC\t<*>\t0\t.\tDP=1;ADF=1,0;ADR=0,0;DPR=1,0;I16=1,0,0,0,36,1296,0,0,37,1369,0,0,12,144,0,0;QS=1,0;MQ0F=0\tPL:DP:DP4:AD\t0,3,36:1:1,0,0,0:1,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\n+17\t4090\t.\tC\t<*>\t0\t.\tDP=1;ADF=1,0;ADR=0,0;DPR=1,0;I16=1,0,0,0,33,1089,0,0,37,1369,0,0,11,121,0,0;QS=1,0;MQ0F=0\tPL:DP:DP4:AD\t0,3,33:1:1,0,0,0:1,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\n+17\t4091\t.\tT\t<*>\t0\t.\tDP=1;ADF=1,0;ADR=0,0;DPR=1,0;I16=1,0,0,0,36,1296,0,0,37,1369,0,0,10,100,0,0;QS=1,0;MQ0F=0\tPL:DP:DP4:AD\t0,3,36:1:1,0,0,0:1,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\n+17\t4092\t.\tG\t<*>\t0\t.\tDP=1;ADF=1,0;ADR=0,0;DPR=1,0;I16=1,0,0,0,37,1369,0,0,37,1369,0,0,9,81,0,0;QS=1,0;MQ0F=0\tPL:DP:DP4:AD\t0,3,37:1:1,0,0,0:1,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\n+17\t4093\t.\tC\t<*>\t0\t.\tDP=1;ADF=1,0;ADR=0,0;DPR=1,0;I16=1,0,0,0,35,1225,0,0,37,1369,0,0,8,64,0,0;QS=1,0;MQ0F=0\tPL:DP:DP4:AD\t0,3,35:1:1,0,0,0:1,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\n+17\t4094\t.\tT\t<*>\t0\t.\tDP=1;ADF=1,0;ADR=0,0;DPR=1,0;I16=1,0,0,0,40,1600,0,0,37,1369,0,0,7,49,0,0;QS=1,0;MQ0F=0\tPL:DP:DP4:AD\t0,3,37:1:1,0,0,0:1,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\n+17\t4095\t.\tA\t<*>\t0\t.\tDP=1;ADF=1,0;ADR=0,0;DPR=1,0;I16=1,0,0,0,35,1225,0,0,37,1369,0,0,6,36,0,0;QS=1,0;MQ0F=0\tPL:DP:DP4:AD\t0,3,35:1:1,0,0,0:1,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\n+17\t4096\t.\tC\t<*>\t0\t.\tDP=1;ADF=1,0;ADR=0,0;DPR=1,0;I16=1,0,0,0,32,1024,0,0,37,1369,0,0,5,25,0,0;QS=1,0;MQ0F=0\tPL:DP:DP4:AD\t0,3,32:1:1,0,0,0:1,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\n+17\t4097\t.\tA\t<*>\t0\t.\tDP=1;ADF=1,0;ADR=0,0;DPR=1,0;I16=1,0,0,0,35,1225,0,0,37,1369,0,0,4,16,0,0;QS=1,0;MQ0F=0\tPL:DP:DP4:AD\t0,3,35:1:1,0,0,0:1,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0:0,0,0,0:0,0\t0,0,0:0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