| Next changeset 1:8c3472790020 (2015-04-21) |
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added:
macros.xml samtools_bedcov.xml test-data/eboVir3.1.bed test-data/eboVir3.2.bam test-data/eboVir3.2.bed test-data/eboVir3.bam test-data/samtools_bedcov_out1.tab test-data/samtools_bedcov_out2.tab tool_dependencies.xml |
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| diff -r 000000000000 -r c04bee391baa macros.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/macros.xml Wed Mar 18 16:43:50 2015 -0400 |
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| @@ -0,0 +1,21 @@ +<macros> + <xml name="requirements"> + <requirements> + <requirement type="package" version="1.2">samtools</requirement> + <yield/> + </requirements> + </xml> + <xml name="citations"> + <citations> + <citation type="doi">10.1093/bioinformatics/btp352</citation> + </citations> + </xml> + <xml name="version_command"> + <version_command>samtools --version | head -n 1 | awk '{ print $2 }'</version_command> + </xml> + <xml name="stdio"> + <stdio> + <exit_code range="1:" level="fatal" description="Error" /> + </stdio> + </xml> +</macros> |
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| diff -r 000000000000 -r c04bee391baa samtools_bedcov.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/samtools_bedcov.xml Wed Mar 18 16:43:50 2015 -0400 |
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| @@ -0,0 +1,57 @@ +<tool id="samtools_bedcov" name="Calculate read depth" version="1.0.1"> + <description>on BAM files</description> + <macros> + <import>macros.xml</import> + </macros> + <expand macro="requirements"></expand> + <expand macro="version_command"></expand> + <expand macro="stdio"></expand> + <command><![CDATA[ + for bamfile in + #for dataset in $input_bams: + "${dataset}" + #end for + ; do + if [ ! -f \$bamfile.bai ] ; then + ln -s \$bamfile && samtools index `basename \$bamfile` ; + else + ln -s \$bamfile && ln -s \$bamfile.bai ; + fi ; + done ; + samtools bedcov "${input_bed}" + #for dataset in $input_bams: + `basename "${dataset}"` + #end for + > "${output}" + ]]> + </command> + <inputs> + <param name="input_bed" type="data" format="bed" label="BED file" /> + <param name="input_bams" type="data" format="bam" label="BAM file" multiple="true" /> + </inputs> + <outputs> + <data format="tabular" name="output" label="${tool.name} on ${on_string}" /> + </outputs> + <tests> + <test> + <param name="input_bed" value="eboVir3.1.bed" ftype="bed" /> + <param name="input_bams" value="eboVir3.bam" ftype="bam" /> + <output name="output" file="samtools_bedcov_out1.tab" /> + </test> + <test> + <param name="input_bed" value="eboVir3.1.bed" ftype="bed" /> + <param name="input_bams" value="eboVir3.bam,eboVir3.2.bam" ftype="bam" /> + <output name="output" file="samtools_bedcov_out2.tab" /> + </test> + </tests> + <help> +**What it does** + +This tool runs the ``samtools bedcov`` command in the SAMtools toolkit. + +Show read depth per BED region. + + </help> + <expand macro="citations"></expand> +</tool> + |
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| diff -r 000000000000 -r c04bee391baa test-data/eboVir3.1.bed --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/eboVir3.1.bed Wed Mar 18 16:43:50 2015 -0400 |
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| @@ -0,0 +1,3 @@ +eboVir3 500 1500 +eboVir3 1500 2000 +eboVir3 1500 3000 |
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| diff -r 000000000000 -r c04bee391baa test-data/eboVir3.2.bam |
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| Binary file test-data/eboVir3.2.bam has changed |
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| diff -r 000000000000 -r c04bee391baa test-data/eboVir3.2.bed --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/eboVir3.2.bed Wed Mar 18 16:43:50 2015 -0400 |
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| @@ -0,0 +1,3 @@ +eboVir3 500 1500 +eboVir3 1500 2000 +eboVir3 1500 3000 |
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| diff -r 000000000000 -r c04bee391baa test-data/eboVir3.bam |
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| Binary file test-data/eboVir3.bam has changed |
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| diff -r 000000000000 -r c04bee391baa test-data/samtools_bedcov_out1.tab --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/samtools_bedcov_out1.tab Wed Mar 18 16:43:50 2015 -0400 |
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| @@ -0,0 +1,3 @@ +eboVir3 500 1500 7919 +eboVir3 1500 2000 3009 +eboVir3 1500 3000 9986 |
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| diff -r 000000000000 -r c04bee391baa test-data/samtools_bedcov_out2.tab --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/samtools_bedcov_out2.tab Wed Mar 18 16:43:50 2015 -0400 |
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| @@ -0,0 +1,3 @@ +eboVir3 500 1500 7919 2094 +eboVir3 1500 2000 3009 1064 +eboVir3 1500 3000 9986 3455 |
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| diff -r 000000000000 -r c04bee391baa tool_dependencies.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool_dependencies.xml Wed Mar 18 16:43:50 2015 -0400 |
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| @@ -0,0 +1,6 @@ +<?xml version="1.0"?> +<tool_dependency> + <package name="samtools" version="1.2"> + <repository changeset_revision="6eea04363026" name="package_samtools_1_2" owner="iuc" toolshed="https://toolshed.g2.bx.psu.edu" /> + </package> +</tool_dependency> |