Repository 'ivar_consensus'
hg clone https://toolshed.g2.bx.psu.edu/repos/iuc/ivar_consensus

Changeset 4:78bbd17d0703 (2020-06-22)
Previous changeset 3:7f781286b55f (2020-06-05) Next changeset 5:5e668dc9f379 (2021-05-19)
Commit message:
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ivar/ commit 693df287d23b0fd9dfd134b41d401a438c3f5ad6"
added:
completemask.py
sanitize_bed.py
test-data/covid19/ARTIC-V1-bad.bed
b
diff -r 7f781286b55f -r 78bbd17d0703 completemask.py
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/completemask.py Mon Jun 22 07:31:22 2020 -0400
[
@@ -0,0 +1,28 @@
+#!/usr/bin/env python
+
+import sys
+
+
+if __name__ == '__main__':
+    with open(sys.argv[1]) as i:
+        getmasked_output = i.readline().strip()
+
+    if not getmasked_output:
+        print()
+        print('No affected primer binding sites found!')
+    else:
+        masked_primers = getmasked_output.split('\t')
+        with open(sys.argv[2]) as i:
+            amplicon_data = [line.strip().split('\t') for line in i]
+
+        masked_complete = []
+        for primer in masked_primers:
+            for amplicon in amplicon_data:
+                if primer in amplicon:
+                    masked_complete += amplicon
+        result = '\t'.join(sorted(set(masked_complete)))
+        print()
+        print('Removing reads primed with any of:')
+        print(result)
+        with open(sys.argv[1], 'w') as o:
+            o.write(result + '\n')
b
diff -r 7f781286b55f -r 78bbd17d0703 sanitize_bed.py
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/sanitize_bed.py Mon Jun 22 07:31:22 2020 -0400
[
@@ -0,0 +1,20 @@
+#!/usr/bin/env python
+
+import sys
+
+
+with open(sys.argv[1]) as i:
+    bed_data = i.readlines()
+
+sanitized_data = []
+try:
+    for record in bed_data:
+        fields = record.split('\t')
+        sanitized_data.append(
+            '\t'.join(fields[:4] + ['60'] + fields[5:])
+        )
+except IndexError:
+    pass  # leave column number issue to getmasked
+else:
+    with open(sys.argv[1], 'w') as o:
+        o.writelines(sanitized_data)
b
diff -r 7f781286b55f -r 78bbd17d0703 test-data/covid19/ARTIC-V1-bad.bed
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/covid19/ARTIC-V1-bad.bed Mon Jun 22 07:31:22 2020 -0400
b
b'@@ -0,0 +1,196 @@\n+MN908947.3\t30\t54\tnCoV-2019_1_LEFT\tNOINT\t+\n+MN908947.3\t385\t410\tnCoV-2019_1_RIGHT\tNOINT\t-\n+MN908947.3\t320\t342\tnCoV-2019_2_LEFT\tNOINT\t+\n+MN908947.3\t704\t726\tnCoV-2019_2_RIGHT\tNOINT\t-\n+MN908947.3\t642\t664\tnCoV-2019_3_LEFT\tNOINT\t+\n+MN908947.3\t1004\t1028\tnCoV-2019_3_RIGHT\tNOINT\t-\n+MN908947.3\t943\t965\tnCoV-2019_4_LEFT\tNOINT\t+\n+MN908947.3\t1312\t1337\tnCoV-2019_4_RIGHT\tNOINT\t-\n+MN908947.3\t1242\t1264\tnCoV-2019_5_LEFT\tNOINT\t+\n+MN908947.3\t1623\t1651\tnCoV-2019_5_RIGHT\tNOINT\t-\n+MN908947.3\t1573\t1595\tnCoV-2019_6_LEFT\tNOINT\t+\n+MN908947.3\t1942\t1964\tnCoV-2019_6_RIGHT\tNOINT\t-\n+MN908947.3\t1875\t1897\tnCoV-2019_7_LEFT\tNOINT\t+\n+MN908947.3\t2247\t2269\tnCoV-2019_7_RIGHT\tNOINT\t-\n+MN908947.3\t2181\t2205\tnCoV-2019_8_LEFT\tNOINT\t+\n+MN908947.3\t2568\t2592\tnCoV-2019_8_RIGHT\tNOINT\t-\n+MN908947.3\t2505\t2529\tnCoV-2019_9_LEFT\tNOINT\t+\n+MN908947.3\t2882\t2904\tnCoV-2019_9_RIGHT\tNOINT\t-\n+MN908947.3\t2826\t2850\tnCoV-2019_10_LEFT\tNOINT\t+\n+MN908947.3\t3183\t3210\tnCoV-2019_10_RIGHT\tNOINT\t-\n+MN908947.3\t3144\t3166\tnCoV-2019_11_LEFT\tNOINT\t+\n+MN908947.3\t3507\t3531\tnCoV-2019_11_RIGHT\tNOINT\t-\n+MN908947.3\t3460\t3482\tnCoV-2019_12_LEFT\tNOINT\t+\n+MN908947.3\t3826\t3853\tnCoV-2019_12_RIGHT\tNOINT\t-\n+MN908947.3\t3771\t3795\tnCoV-2019_13_LEFT\tNOINT\t+\n+MN908947.3\t4142\t4164\tnCoV-2019_13_RIGHT\tNOINT\t-\n+MN908947.3\t4054\t4077\tnCoV-2019_14_LEFT\tNOINT\t+\n+MN908947.3\t4428\t4450\tnCoV-2019_14_RIGHT\tNOINT\t-\n+MN908947.3\t4294\t4321\tnCoV-2019_15_LEFT\tNOINT\t+\n+MN908947.3\t4674\t4696\tnCoV-2019_15_RIGHT\tNOINT\t-\n+MN908947.3\t4636\t4658\tnCoV-2019_16_LEFT\tNOINT\t+\n+MN908947.3\t4995\t5017\tnCoV-2019_16_RIGHT\tNOINT\t-\n+MN908947.3\t4939\t4966\tnCoV-2019_17_LEFT\tNOINT\t+\n+MN908947.3\t5296\t5321\tnCoV-2019_17_RIGHT\tNOINT\t-\n+MN908947.3\t5230\t5259\tnCoV-2019_18_LEFT\tNOINT\t+\n+MN908947.3\t5620\t5644\tnCoV-2019_18_RIGHT\tNOINT\t-\n+MN908947.3\t5563\t5586\tnCoV-2019_19_LEFT\tNOINT\t+\n+MN908947.3\t5932\t5957\tnCoV-2019_19_RIGHT\tNOINT\t-\n+MN908947.3\t5867\t5894\tnCoV-2019_20_LEFT\tNOINT\t+\n+MN908947.3\t6247\t6272\tnCoV-2019_20_RIGHT\tNOINT\t-\n+MN908947.3\t6167\t6196\tnCoV-2019_21_LEFT\tNOINT\t+\n+MN908947.3\t6528\t6550\tnCoV-2019_21_RIGHT\tNOINT\t-\n+MN908947.3\t6466\t6495\tnCoV-2019_22_LEFT\tNOINT\t+\n+MN908947.3\t6846\t6873\tnCoV-2019_22_RIGHT\tNOINT\t-\n+MN908947.3\t6718\t6745\tnCoV-2019_23_LEFT\tNOINT\t+\n+MN908947.3\t7092\t7117\tnCoV-2019_23_RIGHT\tNOINT\t-\n+MN908947.3\t7035\t7058\tnCoV-2019_24_LEFT\tNOINT\t+\n+MN908947.3\t7389\t7415\tnCoV-2019_24_RIGHT\tNOINT\t-\n+MN908947.3\t7305\t7332\tnCoV-2019_25_LEFT\tNOINT\t+\n+MN908947.3\t7671\t7694\tnCoV-2019_25_RIGHT\tNOINT\t-\n+MN908947.3\t7626\t7651\tnCoV-2019_26_LEFT\tNOINT\t+\n+MN908947.3\t7997\t8019\tnCoV-2019_26_RIGHT\tNOINT\t-\n+MN908947.3\t7943\t7968\tnCoV-2019_27_LEFT\tNOINT\t+\n+MN908947.3\t8319\t8341\tnCoV-2019_27_RIGHT\tNOINT\t-\n+MN908947.3\t8249\t8275\tnCoV-2019_28_LEFT\tNOINT\t+\n+MN908947.3\t8635\t8661\tnCoV-2019_28_RIGHT\tNOINT\t-\n+MN908947.3\t8595\t8619\tnCoV-2019_29_LEFT\tNOINT\t+\n+MN908947.3\t8954\t8983\tnCoV-2019_29_RIGHT\tNOINT\t-\n+MN908947.3\t8888\t8913\tnCoV-2019_30_LEFT\tNOINT\t+\n+MN908947.3\t9245\t9271\tnCoV-2019_30_RIGHT\tNOINT\t-\n+MN908947.3\t9204\t9226\tnCoV-2019_31_LEFT\tNOINT\t+\n+MN908947.3\t9557\t9585\tnCoV-2019_31_RIGHT\tNOINT\t-\n+MN908947.3\t9477\t9502\tnCoV-2019_32_LEFT\tNOINT\t+\n+MN908947.3\t9834\t9858\tnCoV-2019_32_RIGHT\tNOINT\t-\n+MN908947.3\t9784\t9806\tnCoV-2019_33_LEFT\tNOINT\t+\n+MN908947.3\t10146\t10171\tnCoV-2019_33_RIGHT\tNOINT\t-\n+MN908947.3\t10076\t10099\tnCoV-2019_34_LEFT\tNOINT\t+\n+MN908947.3\t10437\t10459\tnCoV-2019_34_RIGHT\tNOINT\t-\n+MN908947.3\t10362\t10384\tnCoV-2019_35_LEFT\tNOINT\t+\n+MN908947.3\t10737\t10763\tnCoV-2019_35_RIGHT\tNOINT\t-\n+MN908947.3\t10666\t10688\tnCoV-2019_36_LEFT\tNOINT\t+\n+MN908947.3\t11048\t11074\tnCoV-2019_36_RIGHT\tNOINT\t-\n+MN908947.3\t10999\t11022\tnCoV-2019_37_LEFT\tNOINT\t+\n+MN908947.3\t11372\t11394\tnCoV-2019_37_RIGHT\tNOINT\t-\n+MN908947.3\t11306\t11331\tnCoV-2019_38_LEFT\tNOINT\t+\n+MN908947.3\t11668\t11693\tnCoV-2019_38_RIGHT\tNOINT\t-\n+MN908947.3\t11555\t11584\tnCoV-2019_39_LEFT\tNOINT\t+\n+MN908947.3\t11927\t11949\tnCoV-2019_39_RIGHT\tNOINT\t-\n+MN908947.3\t11863\t11889\tnCoV-2019_40_LEFT\tNOINT\t+\n+MN908947.3\t12234\t12256\tnCoV-2019_40_RIGHT\tNOINT\t-\n+MN908947.3\t12110\t12133\tnCoV-2019_41_LEFT\tNOINT\t+\n+MN908947.3\t12465\t12490\tnCoV-2019_41_RIGHT\tNOINT\t-\n+M'..b'\tNOINT\t+\n+MN908947.3\t18036\t18062\tnCoV-2019_59_RIGHT\tNOINT\t-\n+MN908947.3\t17966\t17993\tnCoV-2019_60_LEFT\tNOINT\t+\n+MN908947.3\t18324\t18348\tnCoV-2019_60_RIGHT\tNOINT\t-\n+MN908947.3\t18253\t18275\tnCoV-2019_61_LEFT\tNOINT\t+\n+MN908947.3\t18650\t18672\tnCoV-2019_61_RIGHT\tNOINT\t-\n+MN908947.3\t18596\t18618\tnCoV-2019_62_LEFT\tNOINT\t+\n+MN908947.3\t18957\t18979\tnCoV-2019_62_RIGHT\tNOINT\t-\n+MN908947.3\t18896\t18918\tnCoV-2019_63_LEFT\tNOINT\t+\n+MN908947.3\t19275\t19297\tnCoV-2019_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