This tool uses the SAMtools toolkit to remove potential PCR duplicates: if multiple read pairs have identical external coordinates, only retain the pair with highest mapping quality. In the paired-end mode, this command ONLY works with FR orientation and requires ISIZE is correctly set. It does not work for unpaired reads (e.g. two ends mapped to different chromosomes or orphan reads). |
hg clone https://toolshed.g2.bx.psu.edu/repos/devteam/samtools_rmdup
Repository package_ncurses_5_9 revision 335ebf512407 owned by iuc (prior install required) |
Repository package_zlib_1_2_8 revision 411985b46ae8 owned by iuc (prior install required) |
Name | Version | Type | |
---|---|---|---|
samtools | 1.2 | package |
Name | Description | Version | Minimum Galaxy Version |
---|---|---|---|
remove PCR duplicates | 2.0 | any |