Sailfish is a tool for transcript quantification from RNA-seq data. It requires a set of target transcripts (either from a reference or de-novo assembly) to quantify. All you need to run sailfish is a fasta file containing your reference transcripts and a (set of) fasta/fastq file(s) containing your reads. Sailfish runs in two phases; indexing and quantification. The indexing step is independent of the reads, and only needs to be run once for a particular set of reference transcripts and choice of k (the k-mer size). The quantification step, obviously, is specific to the set of RNA-seq reads and is thus run more frequently Additional covariates may optionally be provided to further inform the peak-calling process. https://github.com/kingsfordgroup/sailfish |
hg clone https://toolshed.g2.bx.psu.edu/repos/bgruening/sailfish
Repository package_boost_1_57 revision a0dd39685fce owned by iuc (prior install required) |
Repository package_cmake_3_2_3 revision 1aea698a1c63 owned by iuc (prior install required) |
Repository package_bzlib_1_0 revision af4887e1f595 owned by iuc (prior install required) |
Name | Version | Type | |
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sailfish | 0.7.6 | package |
Name | Description | Version | Minimum Galaxy Version |
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transcript quantification from RNA-seq data | 0.7.6.0 | any |